cDNA-AFLP改良法分离草菇低温诱导表达基因和草菇ras启动子的克隆
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摘要
本研究利用cDNA-AFLP改良法分离了13个草菇菌丝低温诱导表达基因v1，v2，v3，v4，v5，v6，v7，v8，v9，v10，v11，v12，v13。DNA序列测定结果表明，V1为488bp，V2为551bp，V3为557bp，V4为285bp，V5为173bp，V6为604bp，V7为743bp，V8为836bp，V9为709bp，V10大约为720bp(未测完)，V11和V12是同一基因片段为1077bp，V13为617bp。BLASTn搜索结果表明V1，V2，V3，V4，V5，V6，V7，V9，V10，V11，V12，V13为未知新基因，V8与ATP酶同源率较高，在一段长312bp的区域，与ATP酶的同源率达84-93％。BLASTx搜索结果表明：V1，V2，V3，V4，V6，V8，V11(V12)，V13的mRNA翻译产物能够找到同源率较高的蛋白，其中六个基因片段，V1，V2，V3，V4，V8，V11的mRNA翻译产物与其他真菌蛋白的同源率较高。V3的mRNA翻译产物与灰盖鬼伞细胞色素P450的同源率为33％，V8的mRNA翻译产物与粗糙脉胞菌的ca~(2+)-ATP酶同源性为68％。
本研究以草菇基因组DNA为模板，设计了一对引物(rasR1，rasF1)，利用PCR技术克隆了草菇的一段启动子片段r5a。DNA序列测定结果及DNA序列对比结果显示：该启动子序列(r5a)与已知的食用菌ras启动子同源率低，采用Plant Prediction和Plant Care软件对克隆的草菇启动子片段进行分析，结果表明该启动子序列的243-293bp及539-589bp区域为预测的基础启动子区，有两个转录起始位点(283bp及579bp处)，有多种重要的顺式作用元件，如TATA-box，CAAT-box，G-box，ABRE元件，WUN-motif，HSE元件，P-box，TATC-Vbox，I-box等。
Using a single restriction enzyme based on cONA-AFLP to isolate the differential gene, we got 13 differentially expressed gene fragments, we named them, Vl(488bp), V2(551bp), V3(557bp), V4(285bp), V5(173bp), V6(604bp), V7(743bp), V8(836bp), V9(709bp), V10(about720bp), V11(1077bp), V12(l077bp), V13(617bp). Vll and V12 are the same gene fragments. Using BLASTn to analyse these sequences, we got the following results: VI, V2, V3, V4, V5, V6, V7, V9, V10, 11(V12), V13 are the unknown gene. V8 is 84-93% the same as ATPase gene in a region of 312bp. Using BLASTx, we know that: V1, V2, V3, V4, V11(V12), V13 are more than 30% the same as the protein of other fungus. V3 is about 33% the same as cytochrome P450(from Coprinopsis cinerea ), V8 is about 68% the same as ca2+-ATPase (from Neurospora crassa).
Using PCR technology to isolate ras promoter, we got a DMA sequence that is likely to be a promoter sequence. This DNA sequence is not the same as other ras promoter sequences in edible mushroom. Two possible foundational promoter sequences locate on the region of 243-293bp and 539-589bp.There are two transcription start points. One locate at the site of 283bp,another locate at the site of 579bp. There are also many important cis-acting regulatory elements, such as TATA-box, CMT-box, G-box, ABRE, WUN-motif, HSE, P-box, TATC-box, I-box et al.
本研究以草菇基因组DNA为模板，设计了一对引物(rasR1，rasF1)，利用PCR技术克隆了草菇的一段启动子片段r5a。DNA序列测定结果及DNA序列对比结果显示：该启动子序列(r5a)与已知的食用菌ras启动子同源率低，采用Plant Prediction和Plant Care软件对克隆的草菇启动子片段进行分析，结果表明该启动子序列的243-293bp及539-589bp区域为预测的基础启动子区，有两个转录起始位点(283bp及579bp处)，有多种重要的顺式作用元件，如TATA-box，CAAT-box，G-box，ABRE元件，WUN-motif，HSE元件，P-box，TATC-Vbox，I-box等。
Using a single restriction enzyme based on cONA-AFLP to isolate the differential gene, we got 13 differentially expressed gene fragments, we named them, Vl(488bp), V2(551bp), V3(557bp), V4(285bp), V5(173bp), V6(604bp), V7(743bp), V8(836bp), V9(709bp), V10(about720bp), V11(1077bp), V12(l077bp), V13(617bp). Vll and V12 are the same gene fragments. Using BLASTn to analyse these sequences, we got the following results: VI, V2, V3, V4, V5, V6, V7, V9, V10, 11(V12), V13 are the unknown gene. V8 is 84-93% the same as ATPase gene in a region of 312bp. Using BLASTx, we know that: V1, V2, V3, V4, V11(V12), V13 are more than 30% the same as the protein of other fungus. V3 is about 33% the same as cytochrome P450(from Coprinopsis cinerea ), V8 is about 68% the same as ca2+-ATPase (from Neurospora crassa).
Using PCR technology to isolate ras promoter, we got a DMA sequence that is likely to be a promoter sequence. This DNA sequence is not the same as other ras promoter sequences in edible mushroom. Two possible foundational promoter sequences locate on the region of 243-293bp and 539-589bp.There are two transcription start points. One locate at the site of 283bp,another locate at the site of 579bp. There are also many important cis-acting regulatory elements, such as TATA-box, CMT-box, G-box, ABRE, WUN-motif, HSE, P-box, TATC-box, I-box et al.
引文
1．张树庭。草菇。香港中文大学出版社，1975：27。
2. Toyama, N. & Ogawa, K. Comparative studies on cellulolytic and oxidizing enzyme activities of edible and inedible wood rotters. Mushroom science, 1974,9:745-746.
3. Chang, S.T. and Yau, C.K. Volvariella volvacea and its life history. American Journal of Botary, 1971,58:552-556.
4. Chiu, S.W. Evidence for a haploid life-cycle in Volvariella volvacea from microspectrophotometric measurements and observations of nuclear behaviour. Mycological Research, 1993,97:1481-1485.
5. Chang S.T. and Li Shuxian. Genetical studies on the sexuality pattern of Volvariella volvacea .Science and Cultivation of edible fungi. Proceedings of the 13th International Congress on the Science and Cultivation of edible fungi. 1991,(1):119-122.
6．林梦藻。草菇厚垣孢子细胞学观察。食用菌。1991，(1)：43。
7. Chang, S.T. and Chu, S.S. Nuclear behavior in the basidium of Volvariella volvacea cytologia, 1969. 34:293-299.
8．陈明杰，赵绍惠，张树庭。草菇染色体核型的鉴定。食用菌学报，1995，2(1)：1-6．
9．徐天惠，刘强。食用菌概论。中国展望出版社，1987：233-238。
10. Chang, S.T. and Miles, P.G. Edible mushrooms and their cultivation. CRC Press, Florida, 1989:120-150.
11．薛卫东。果蔬贮藏与保鲜。成都电子科技大学出版社。1996，283-284。
12. Chang S.T., Hayes W.A., Volvariella volvacea in the biology and cultivation of edible fungi. Academic press .New York. 1978:573-605.
13．张树庭。草菇。香港中文大学出版社，1975：143-147。
14．段学武等。草菇低温贮藏及有关生理变化研究。热带作物学报，2000，21(4)：75-79。
15．王富民等。草菇保鲜生理研究。食用菌，1990，12(6)：37-38。
16．王富民等。草菇保鲜及有关生理变化。上海农业学报，1992，8(3)：60-66。
17. Chang, S.T., Quimil, T.H.. Tropical mushrooms biological nature and cultivation
methods. Hong kong: The Chinese University Press, 1982:69-80.
18．李志超。金针菇、草菇栽培技术。山西科学技术出版社，1993：166。
19．杨新美。中国食用菌栽培学。农业出版社，1988：412-413。
20．谢宝贵等。草菇厚垣孢子的研究。食用菌学报，1996，3(4)：25-29。
21．蒲丽珍等。草菇保鲜试验。食用菌，1991，(5)：38。
22．周崇俊等。草菇辐射储藏保鲜研究。食用菌，1988，(1)：39。
23. Tyshenko MG, Doucet D, Daviest PL and Walker VK. The antifreeze potential of the spruce budworm thermal hysteresis protein. Nature Biotechnology, 1997,15:887-890.
24. Graham LA, Liou YC, Walker CK, Davies PL. Hyperactive antifreeze protein from beetles. Nature, 1997,388:727-728.
25. Choy Hew. Insect antifreezes come in from the cold. Nature Biotechnology, 1997,15 (9): 844.
26．陈明杰等。低温影响草菇蛋白质组份变化的研究。食用菌学报，1995，2(4)：28-31。
27．陈明杰等。低温引起草菇菌丝体RNA变化的研究。食用菌学报，1997，4(2)：16-18。
28．陈明杰等。草菇菌丝体mRNA在低温条件下的研究。农业生物技术学报，1997，5(2)：185-187。
29．陈明杰等。草菇低温诱导基因的分离。菌物系统，1998，17(4)：327-330。
30．张蒙等。草菇低温诱导蛋白的初步研究。食用菌学报，2000，7(1)：56-58。
31．吴乃虎，《基因工程原理》，科学出版社，2000年1月第二版。
32. Ouvrard, cellier F et al. Identification and expression of water stress and abscisic acid regulated genes in a drought tolerant sunflower genotype. Plant Molecular Biology, 1996,31(4): 819-829
33. Atanassov I, Russinova E, et al. Expression of an anther specific chalcone synthase -like gene is correlated with uninucleate microspore development in Nicotiana sylvestris. Plant Molecular Biology, 1998,38(6);1169-1178
34. Bouillet P. Oulad Abdelghani M, et al. Efficient cloning of cDNAs of retinoic acid-responsive genes in P19 embryonal carcinoma cells and characterization of a novel mouse gene. Developmental Biology, 1995, 170(2):420-433
35. Sato S, Toya T, et al. Isolation of a carrot gene expressed specifically during
early-stage somatic embryogenesis. Plant Molecular Biology,1995,28(1): 39-46.
36. Lisitsyn N, Wiglwer M. Science, 1992,259-946.
37. Hubark M, Schatz D G. Nuxleic Acids Rerearch, 1994,22: 5560
38. Melia M J, Bofill N, et al. Identification of androgen regulated genes in mouse kidney by representational difference analyses and random arbitrarily primed polymerase chain reaction. Endocrinology Philadelphia, 1998,139(2): 688-695.
39. Butler E, Gallagher T F. Gene expression during adventitious root formation in apple. Symposia of the Society for Experimental Biology ,1998,51:79-84
40. Zeng J et al. 1994. Nucleic Acid Res. 1994,22:4381-4385
41. Yang M et al. Anal Biochem, 1996,237:109-114
42. Diachenko L B, et al. Combining the technique of RNA fingerprinting and differential display to obtain differentially expressed mRNA. Biochem Biophys Res Commun, 1996,219:824-828
43. Diatchenko K J et al. Pro Natl Acad Sci, 1996, 93: 6025-6030
44. Oliver Dvon Stein, wolf Gerolf Thies, et al. A high throughput screening for rarely transcribed differentially expressed genes. Nucleic Acids Research, 1997,25(13):2598-2602
45. Birch PRJ, Avrova AO, et al. Isolation of potato genes that are induced during an early stage of the hypersensitive response to phytophthora infestans. Molecular Plant Microbe Interactions, 1999,12(4):356-361
46. Wu Wenxuan, Qi Zhang,et al. Suppression subtractive hybridization identified a marked increase in thrombospondin-1 associated with parturition in pregnant sheep myometrium. Endocrinology Philadelphia, 1999, 140(5): 2364-2371
47. Liang P and Pardee AB. Differential display of eukaryoti messenger RNA by means of the polymerase chain reaction. Science, 1992, 257:967-971
48. Welsh J, Chada K et al. Arbitrarily primed PCR fingerprinting. Nucleic Acids Research ,1992,20: 4865-4970
49．李子银等。水稻盐胁应答基因的克隆、表达及染色体定位。中国科学。1999，29(6)：561-571。
50．赵大中等．科学通报．1998，43(9)：965-968
51．张驰等．利用DDRT-PCR技术分析盐胁迫下水稻耐盐突变体中特异表达的基因。中国科学．1995，25(B)：840-847
52. Jack Q, et al. Plant Molecular Biology, 1995,27:1097-1108
53. MeClelland M, et al. RNA fingerprinting and differential display using arbitrarily primed PCR. Trends Genet, 1995,11:242-246
54. Bachem CWB et al. Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: analysis of gene expression during potato tuber development .The Plant J, 1996,9(5):745-753
55. Bauer D, et al. PCR methods and applications manual supplement, ppS805-809. Cold Spring Harbor University Press, 1994.
56. Zhao S, et al. Nucl Acids Res, 1992,20:4965-4970
57. Ivanova NB and Belyavsky AV. Identification of differentially expressed genes by restriction endonuclease-based gene expression fingerprinting. Nucleic Acids Research, 1995, 23:2954-2958
58. Kato K. RNA fingerprinting by molecular indexing. Nucleic Acids Research, 1996, 24: 394-395
59. Money T, Reader S et al. AFLP-based mRNA fingerprinting. Nucleic Acids Research, 1996, 24: 2616-2617
60. Pieter Vos, et al. AFLP: anew technique for DNA fingerprinting. Nucleic Acids Research, 1995, vol. 23 No. 21 4407-4414
61. Christian W.B et al. Plant Molecular Biology Reporter, 1998,16:157-173
62. Hubu Y et al. Amplified restriction fragment length polymorphism-based mRNA fingerprinting using a single restriction enzyme that recognizes a 4-bp sequence. Biochem Biophys Res Comn, 1997,234:516-521
63. Bachem CWB, Oomen RJFJ, Visser RGF. Transcript imaging with cDNA-AFLP: a step by step protocol. Plant Molecular Biology Reporter, 16:157-173.
64. Suarez M C, et al. Developing expressed sequence tags from polymorphic trascript-derived fragments in cassava. GENOME, 43: 62-67.
65．吴敏生等。利用cDNA-AFLP技术研究玉米基因的差异表达。作物学报，2001，27(3)：39-42。
66. Bachem CWB et al. Plant J, 2001,25(6):595-604
67. Campalans A, Pages M, Messeguer R. Tree Physiol 2001 Jul: 21(10): 633-43
68. Vanderbiezen E A, Juwana H, Parker J E, Jones J D G. cDNA-AFLP display for the isolation of Peronospora Parasitiea genes expressed during infection in Arabidopsis thaliana. Molecular Plant Merobe Interactions, 2000, 13(8): 895-899.
69. Qin L, et al. An efficient eDNA-AFLP-based strategy for the identification of putative pathogenicity factors from the potato eystnematode Globodera Rostoehiensis. Molecular Plant Merobe Interactions, 2000,13(8):830-836.
70. Dellagi A, et al. eDNA-AFLP analysis of differential gene expression in the prokaryotie plant pathogen Erwinia Carotovora. Merobiology, 2000, 146: 165-171.
71. Kojima T, Habu Y, et al. Direct isolation of differentially expressed genes from a specific chromosome region of common wheat _Application of the amplified fragment length polymorphism-based messenger RNA fingerprinting method in combination with a deletion line of wheat. Molecular and Ceneties, 263(4): 635-641.
72. Fukuda T, et al. Cloning of differentially expressed genes in highly and low metastatic rat Osteosarcomas by modified cDNA-AFLP method. Biochemical and Biophysical Research Communications, 1999, 261(1): 35-40.
73. Sambrook J, FritsehE F, Maniatis T. Moleeular Cloning:A Laboratory Manual. 2nd ed New York: Cold Spring Harbor Labortory press, 1989.]
74. 韩志勇等。一种改良的质粒DNA小量提取法(A Modified Method for Miniprep of Plasmid DNA)。生特技术通报(Biotechnology Information),2000,4: 45-47。
75. Asperger and H-Pkleber. Distribution and Diversity of Bacterial eytoehromes P-450|A|.In: Ruckpaal, K. et al eds. Mierobial and plant eytoehrome|c|. Lodon: Taylor and Francis, 1991, 1-53.
76. Dursl F. Bioehemistry and physiology of plant eytoehrome[c] London: Taylor and Francis, 1991,193-233.
77. Chapple CC.A cDNA encoding a novel eytoehrome P450-dependent monooxygenase from
Arabidopsis thaliana. Plant Physiology, 1995,108(2): 875-876.
78. Nooden LD, Genetic control of senescence and aging in plants. In: Handbook of the Biology of Aging (Edition 4). Edited by Schneider EL, Rowe JW. Orland: Academic Press, 1996: 94-118.
79. Bush DS. Regulation of eytosolic calcium in plants. Plant Physiol, 1993,103:7-13.
80. Minorsky PLAn heuristic hypothesis of chilling injury on plants. A role for calcium as the primary physiological transducer of injury. Plant Cell Environ, 8:75-82.
81. Askerlund P, Sommarin M. Caleium efflux transporters in higher plants. In:Smallwood M, Knox JP, Bowles DJ(eds).Membranes:Specialized Function in Plants. Oxford, UK: BIOS Scientific Publishers Ltd, 281-299.
82. Poovaiah BW, Reddy ASN. Calcium and signal transcuction in plants. Crit Rev Plant Sci, 12:185-211.
83. Minorsky PV.A heuristic hypothesis of chilling injury in plants: a role for caleiumas the primary physiological transducer of injury. Plant Cell Environ, 1985,8:75-94.
84. Wang H(王红), Jian LC(简令成)。Changes of the level of Ca~(2+). in the cells of rice seedlings under low temperature stress. Acta Bot Sin(植物学报), 36:587-591.
85. Bush DS. Calcium regulation in plants, cells and its role in signaling. Annu Rev Plant Physiol Plant Mol Biol, 46:95-122.
86. Jian LC, Li JH, Chen WP, Li PH, Ahlstrand GG. Cytochemical localization of calcium and Ca~(2+)-ATPase activity under chilling stress:a eompative study between the chilling sensitive maize and the chilling insensitive winter wheat. Plant Cell Physio1,1999,40:1061-1071.
87. Jian LC(简令成)。The brief review about the topic "The cytological study on the mechanism of chilling resistant in plant" in past forty years. Chin bull Bot(植物学通报), 16(Suppl): 15-19.
88. Perez Prat E, Narasimhan ML, Binzel ML, Botella MA, Chen Z, Valpusta V, Bressan RA, Hasegawa PM. Induction of a putative Ca~(2+)-ATPase mRNA in NaCl-adapted
cells. Plant Physiol, 1992, 100: 1471-1478.
89. Zeng SX(曾韶西), LI MR. (李美如). Changes of Ca~(2+)-ATPase activities in cell of rice seedling during the enhancement of chilling resistance induced by cold and salt pretreatment. Acta Bot Sin(植物学报), 1999,41:156-160.
90. Li MR (李美如), et al. Effect of calcium on the cold-resistance of rice seedlings. Acta Phytophysiol Sin (植物生理学报), 1996, 22: 379-384.
91．林善枝等．毛白杨幼苗低温锻炼过程中Ca~(2+)的作用及细胞Ca~(2+)-ATP酶活性的变化．植物生理与分子生物学学报．2002，28(6)：449-456．
92. Li MR (李美如), et al. Antifreezing substances in plant cell and relation to cold resistance. Plant Physiol Commun, 1995, 31(5):328-334.
93. Tawfik AA, Palta J. Evidence for the effect of in vivo calcium concentration on plasma membrane ATPase activity. Plant Physiol, 1993, 102(1): 108-120.
94. Monroy A, Sarhan F, Dhindsa R. Cold-induced changes in freezing tolerance, protein phosphorylation and gene expresion. Evidence for a rode of calcium. Plant Physiol, 1993,102:1227-1234.
95. Rachael LN, Robert WW, Raymond LR. Eukaryotic DNA fragments which act as Promoters for a plasmid gene [J]. Nature, 979, 77: 24-325.
96．徐国洲等。酵母 K.cicerisporus基因组中有启动子功能的DNA片段的克隆。生物化学与生物物理学报，1998，30(1)：35-40。
97．梁明山等。海甘蓝基因启动子的分离和鉴定。中国油料作物学报，1998，20：1-6。
98．徐恒等。大肠杆菌-分枝杆菌启动子探针型穿梭载体pEQ3的构建。四川大学学报，1997，34(3)：349-353。
99．A azaz Abderranhmane等。一种具有高活性的酪氨酸酶基因启动子片段的分离。武汉大学学报，1999，45(2)：233-237。
100．张宪银等。水稻胚乳特异性启动子Gt1的克隆及其功能验证。作物学报，2002，28(1)：110-114。
101．马志刚等。马铃薯prp1-1启动子的克隆及转基因研究。西南农业学报，2002，15(1)：12-14。
102．罗克明等。棉花Lea蛋白D-113基因启动子的克隆及序列分析。遗传学报，29(2)：161-165。
103．陆海等。银杏木质部特异定位表达基因启动子克隆。北京林业大学学报。
104．李永春等。蔗糖合酶基因启动子克隆及其转基因水稻植物中的特异表达。作物学报，2002，28(5)：586-590。
105．刘复权等。烟草NFL1基因启动子区的克隆和初步的功能分析。北京大学学报(自然科学版)，2000，36(2)：263-267。
106．王新力等。香蕉果实成熟相关基因ACO1启动子区的克隆及其功能初探。生物工程学报，2001，17(4)：428-431。
107. Siebert P D. Chenchik A, Kellogg D E et al. An improved PeR metherod for walking in uncloned genomic
108．邓晓东。华南热带农业大学博士学位论文，2002。
109. Kajiwara S, Shishido K. Characterization of thepromoter region of the basidiomycete Lentunes edodes Le. ras gene. FEMS Microbiology Letters, 1992,92 (2): 147-150.
110. Hirano T, Sato T, Okawa K, et al. Isolation and characterization of the glyceraldehydes-3-phosphate dehydrogenase gene of Lentinus edodes. Bioscience biotechnology and Biochemistry, 1999, 63(7): 1223-1227.
111. Kingsnorth CS, Eastwood, DC, Burton KS. Cloning and postharvest expression of serine proteinase transcripts in the cultivated mushroom Agaricus bisporus. Fungal Genet. Biol, 2001,32(3):135-144.
112. Stark MJ, Milner JS. Cloning and analysis of the Kluyveromyces lactis TRP1 gene: a chromosomal locus flanded by genes encoding inorganic pyrophosphatase and histone H3. Yeast, 1989m5(1): 35-50.
113. Skrzynia C, Binninger DM, Pukkila PJ, et al. Molecular characterization of TRP1, a gene coding for tryptophan synthetase in the basidiomycete Coprinus cinereus. Gene, 1989, 81(1): 73-82.
114. Raguz S, Yag E, Wood, DA, et al. Isolation and characterization of a cellulose-growth-specific gene from Agaricus bisporus. Gene, 1992, 119(2): 183-190.
115. Yague E, ZunicMM, Morgan L, et al. Expression of eEL 2 and CEL4, two proteins from Agaricus bisporus with similarity to fungal cellobiohydrolase I and
beta-mannanase, respectively, is regulated by the carbon source. Microbiology, 1997, 143: 239-244.
116. Susumu Kajiwara, Kazuyoshi Yamaka, et al. Isolation and sepuence of a developmentally regulated putative novel gene, priA, from the basidiomycete Lentinus edodes. Gene, 1992, 114: 173-178.
117. Zhao J, Chen YH, Kwan HS. Molecular cloning, characterization and differential expression of a glucoamylase gene from the basidiomycetous fungus Lentunus edodes. Applied and Environmental Microbiology, 2000: 2531-2535.
118. Barbacid, M. Annu. Rev. Biochem, 1987(6): 779-827.
119. Schuncn MJ, Peter JP, Maria AP, et al. Function elements in the promoter region of the Aspergillus nidulans gpdA gene encoding plyceraldehydes-3-phosphate dehydrogenase. Gene, 1990,93:101-109.
120. Chen X, Stone M, Schlagnhaufer, et al. A fruiting body tissue method for efficient Agrobacterium-mediated transformation of Agaricus bisporus. Applied and Environmental Microbiology, 2000:4510-4513.
121. Hori, K, Kajiwara S, Saito T, et al. Cloning, sequence analysis and transcriptional expression of aras gene of the edible basidiomycete Lentinus edodes. Gene, 1991, 105(1): 91-96.
122. Osamu Ishibashi, Kazuo Shishido. Nucleaotide sequence of aras gene from the basidiomycete coprinus cinereus. Gene, 1993, 125(2): 233-234.
123. Nagase, T, Ueno, Y, and Ishii, S. Gene, 1990, 94: 249-253.
124. Yamanoto, F, and Perucho, M. Oncogene Res, 1988,3:125-138.
125. Hoffman, E. K, Trusko, S.P, Freeman, N, and George, D.L. Mol, Cell, Biol, 1987(7): 2592-2596.
126. Hall, A. and Brown, R. Nucleic Acids Res, 1985, 13: 5255-5268.
127. Kadonaga, J.T., Carner, K.R., Masiarz, F.R. and Tjian, R. Cell, 1987, 51: 1079-1090.
128. Beeathnach, R. and Chambon, P. Annu. Res. Biochem. 1981,50:249-383.
129. Myers, R.M.,Tilly, k. and Maniatis, T. Science, 1986,232:613-618.
130. Bishop, J.G and Corces, V.G. Gene Der, 1988,2:567-577.
131. Sato T, Yaegashi K, Ishll S, et al. Transformation of the edible basidiomycete Lentinus edodes by restriction enzyme-mediated DNA integration of plasmid DNA. Bioteehnol. Biochem, 1998, 62(12): 2346-2358.
132. Yanai K, Yonekura K, Usami H, et al. The integrative transformationof Pleurotus ostreatus using Biolaphos resistance as a dominant selectable marder. Bioscience Biotechnology Biochemistry, 1996, 60(3): 472-475.
133. Granado JD, Kertesz-Chaloupkova, Aebi M, et al. Restriction enzyme-mediate DNA integration in Coprinus cinereus. Mol. Gen. Genet, 1997, 256: 28-36.
2. Toyama, N. & Ogawa, K. Comparative studies on cellulolytic and oxidizing enzyme activities of edible and inedible wood rotters. Mushroom science, 1974,9:745-746.
3. Chang, S.T. and Yau, C.K. Volvariella volvacea and its life history. American Journal of Botary, 1971,58:552-556.
4. Chiu, S.W. Evidence for a haploid life-cycle in Volvariella volvacea from microspectrophotometric measurements and observations of nuclear behaviour. Mycological Research, 1993,97:1481-1485.
5. Chang S.T. and Li Shuxian. Genetical studies on the sexuality pattern of Volvariella volvacea .Science and Cultivation of edible fungi. Proceedings of the 13th International Congress on the Science and Cultivation of edible fungi. 1991,(1):119-122.
6．林梦藻。草菇厚垣孢子细胞学观察。食用菌。1991，(1)：43。
7. Chang, S.T. and Chu, S.S. Nuclear behavior in the basidium of Volvariella volvacea cytologia, 1969. 34:293-299.
8．陈明杰，赵绍惠，张树庭。草菇染色体核型的鉴定。食用菌学报，1995，2(1)：1-6．
9．徐天惠，刘强。食用菌概论。中国展望出版社，1987：233-238。
10. Chang, S.T. and Miles, P.G. Edible mushrooms and their cultivation. CRC Press, Florida, 1989:120-150.
11．薛卫东。果蔬贮藏与保鲜。成都电子科技大学出版社。1996，283-284。
12. Chang S.T., Hayes W.A., Volvariella volvacea in the biology and cultivation of edible fungi. Academic press .New York. 1978:573-605.
13．张树庭。草菇。香港中文大学出版社，1975：143-147。
14．段学武等。草菇低温贮藏及有关生理变化研究。热带作物学报，2000，21(4)：75-79。
15．王富民等。草菇保鲜生理研究。食用菌，1990，12(6)：37-38。
16．王富民等。草菇保鲜及有关生理变化。上海农业学报，1992，8(3)：60-66。
17. Chang, S.T., Quimil, T.H.. Tropical mushrooms biological nature and cultivation
methods. Hong kong: The Chinese University Press, 1982:69-80.
18．李志超。金针菇、草菇栽培技术。山西科学技术出版社，1993：166。
19．杨新美。中国食用菌栽培学。农业出版社，1988：412-413。
20．谢宝贵等。草菇厚垣孢子的研究。食用菌学报，1996，3(4)：25-29。
21．蒲丽珍等。草菇保鲜试验。食用菌，1991，(5)：38。
22．周崇俊等。草菇辐射储藏保鲜研究。食用菌，1988，(1)：39。
23. Tyshenko MG, Doucet D, Daviest PL and Walker VK. The antifreeze potential of the spruce budworm thermal hysteresis protein. Nature Biotechnology, 1997,15:887-890.
24. Graham LA, Liou YC, Walker CK, Davies PL. Hyperactive antifreeze protein from beetles. Nature, 1997,388:727-728.
25. Choy Hew. Insect antifreezes come in from the cold. Nature Biotechnology, 1997,15 (9): 844.
26．陈明杰等。低温影响草菇蛋白质组份变化的研究。食用菌学报，1995，2(4)：28-31。
27．陈明杰等。低温引起草菇菌丝体RNA变化的研究。食用菌学报，1997，4(2)：16-18。
28．陈明杰等。草菇菌丝体mRNA在低温条件下的研究。农业生物技术学报，1997，5(2)：185-187。
29．陈明杰等。草菇低温诱导基因的分离。菌物系统，1998，17(4)：327-330。
30．张蒙等。草菇低温诱导蛋白的初步研究。食用菌学报，2000，7(1)：56-58。
31．吴乃虎，《基因工程原理》，科学出版社，2000年1月第二版。
32. Ouvrard, cellier F et al. Identification and expression of water stress and abscisic acid regulated genes in a drought tolerant sunflower genotype. Plant Molecular Biology, 1996,31(4): 819-829
33. Atanassov I, Russinova E, et al. Expression of an anther specific chalcone synthase -like gene is correlated with uninucleate microspore development in Nicotiana sylvestris. Plant Molecular Biology, 1998,38(6);1169-1178
34. Bouillet P. Oulad Abdelghani M, et al. Efficient cloning of cDNAs of retinoic acid-responsive genes in P19 embryonal carcinoma cells and characterization of a novel mouse gene. Developmental Biology, 1995, 170(2):420-433
35. Sato S, Toya T, et al. Isolation of a carrot gene expressed specifically during
early-stage somatic embryogenesis. Plant Molecular Biology,1995,28(1): 39-46.
36. Lisitsyn N, Wiglwer M. Science, 1992,259-946.
37. Hubark M, Schatz D G. Nuxleic Acids Rerearch, 1994,22: 5560
38. Melia M J, Bofill N, et al. Identification of androgen regulated genes in mouse kidney by representational difference analyses and random arbitrarily primed polymerase chain reaction. Endocrinology Philadelphia, 1998,139(2): 688-695.
39. Butler E, Gallagher T F. Gene expression during adventitious root formation in apple. Symposia of the Society for Experimental Biology ,1998,51:79-84
40. Zeng J et al. 1994. Nucleic Acid Res. 1994,22:4381-4385
41. Yang M et al. Anal Biochem, 1996,237:109-114
42. Diachenko L B, et al. Combining the technique of RNA fingerprinting and differential display to obtain differentially expressed mRNA. Biochem Biophys Res Commun, 1996,219:824-828
43. Diatchenko K J et al. Pro Natl Acad Sci, 1996, 93: 6025-6030
44. Oliver Dvon Stein, wolf Gerolf Thies, et al. A high throughput screening for rarely transcribed differentially expressed genes. Nucleic Acids Research, 1997,25(13):2598-2602
45. Birch PRJ, Avrova AO, et al. Isolation of potato genes that are induced during an early stage of the hypersensitive response to phytophthora infestans. Molecular Plant Microbe Interactions, 1999,12(4):356-361
46. Wu Wenxuan, Qi Zhang,et al. Suppression subtractive hybridization identified a marked increase in thrombospondin-1 associated with parturition in pregnant sheep myometrium. Endocrinology Philadelphia, 1999, 140(5): 2364-2371
47. Liang P and Pardee AB. Differential display of eukaryoti messenger RNA by means of the polymerase chain reaction. Science, 1992, 257:967-971
48. Welsh J, Chada K et al. Arbitrarily primed PCR fingerprinting. Nucleic Acids Research ,1992,20: 4865-4970
49．李子银等。水稻盐胁应答基因的克隆、表达及染色体定位。中国科学。1999，29(6)：561-571。
50．赵大中等．科学通报．1998，43(9)：965-968
51．张驰等．利用DDRT-PCR技术分析盐胁迫下水稻耐盐突变体中特异表达的基因。中国科学．1995，25(B)：840-847
52. Jack Q, et al. Plant Molecular Biology, 1995,27:1097-1108
53. MeClelland M, et al. RNA fingerprinting and differential display using arbitrarily primed PCR. Trends Genet, 1995,11:242-246
54. Bachem CWB et al. Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: analysis of gene expression during potato tuber development .The Plant J, 1996,9(5):745-753
55. Bauer D, et al. PCR methods and applications manual supplement, ppS805-809. Cold Spring Harbor University Press, 1994.
56. Zhao S, et al. Nucl Acids Res, 1992,20:4965-4970
57. Ivanova NB and Belyavsky AV. Identification of differentially expressed genes by restriction endonuclease-based gene expression fingerprinting. Nucleic Acids Research, 1995, 23:2954-2958
58. Kato K. RNA fingerprinting by molecular indexing. Nucleic Acids Research, 1996, 24: 394-395
59. Money T, Reader S et al. AFLP-based mRNA fingerprinting. Nucleic Acids Research, 1996, 24: 2616-2617
60. Pieter Vos, et al. AFLP: anew technique for DNA fingerprinting. Nucleic Acids Research, 1995, vol. 23 No. 21 4407-4414
61. Christian W.B et al. Plant Molecular Biology Reporter, 1998,16:157-173
62. Hubu Y et al. Amplified restriction fragment length polymorphism-based mRNA fingerprinting using a single restriction enzyme that recognizes a 4-bp sequence. Biochem Biophys Res Comn, 1997,234:516-521
63. Bachem CWB, Oomen RJFJ, Visser RGF. Transcript imaging with cDNA-AFLP: a step by step protocol. Plant Molecular Biology Reporter, 16:157-173.
64. Suarez M C, et al. Developing expressed sequence tags from polymorphic trascript-derived fragments in cassava. GENOME, 43: 62-67.
65．吴敏生等。利用cDNA-AFLP技术研究玉米基因的差异表达。作物学报，2001，27(3)：39-42。
66. Bachem CWB et al. Plant J, 2001,25(6):595-604
67. Campalans A, Pages M, Messeguer R. Tree Physiol 2001 Jul: 21(10): 633-43
68. Vanderbiezen E A, Juwana H, Parker J E, Jones J D G. cDNA-AFLP display for the isolation of Peronospora Parasitiea genes expressed during infection in Arabidopsis thaliana. Molecular Plant Merobe Interactions, 2000, 13(8): 895-899.
69. Qin L, et al. An efficient eDNA-AFLP-based strategy for the identification of putative pathogenicity factors from the potato eystnematode Globodera Rostoehiensis. Molecular Plant Merobe Interactions, 2000,13(8):830-836.
70. Dellagi A, et al. eDNA-AFLP analysis of differential gene expression in the prokaryotie plant pathogen Erwinia Carotovora. Merobiology, 2000, 146: 165-171.
71. Kojima T, Habu Y, et al. Direct isolation of differentially expressed genes from a specific chromosome region of common wheat _Application of the amplified fragment length polymorphism-based messenger RNA fingerprinting method in combination with a deletion line of wheat. Molecular and Ceneties, 263(4): 635-641.
72. Fukuda T, et al. Cloning of differentially expressed genes in highly and low metastatic rat Osteosarcomas by modified cDNA-AFLP method. Biochemical and Biophysical Research Communications, 1999, 261(1): 35-40.
73. Sambrook J, FritsehE F, Maniatis T. Moleeular Cloning:A Laboratory Manual. 2nd ed New York: Cold Spring Harbor Labortory press, 1989.]
74. 韩志勇等。一种改良的质粒DNA小量提取法(A Modified Method for Miniprep of Plasmid DNA)。生特技术通报(Biotechnology Information),2000,4: 45-47。
75. Asperger and H-Pkleber. Distribution and Diversity of Bacterial eytoehromes P-450|A|.In: Ruckpaal, K. et al eds. Mierobial and plant eytoehrome|c|. Lodon: Taylor and Francis, 1991, 1-53.
76. Dursl F. Bioehemistry and physiology of plant eytoehrome[c] London: Taylor and Francis, 1991,193-233.
77. Chapple CC.A cDNA encoding a novel eytoehrome P450-dependent monooxygenase from
Arabidopsis thaliana. Plant Physiology, 1995,108(2): 875-876.
78. Nooden LD, Genetic control of senescence and aging in plants. In: Handbook of the Biology of Aging (Edition 4). Edited by Schneider EL, Rowe JW. Orland: Academic Press, 1996: 94-118.
79. Bush DS. Regulation of eytosolic calcium in plants. Plant Physiol, 1993,103:7-13.
80. Minorsky PLAn heuristic hypothesis of chilling injury on plants. A role for calcium as the primary physiological transducer of injury. Plant Cell Environ, 8:75-82.
81. Askerlund P, Sommarin M. Caleium efflux transporters in higher plants. In:Smallwood M, Knox JP, Bowles DJ(eds).Membranes:Specialized Function in Plants. Oxford, UK: BIOS Scientific Publishers Ltd, 281-299.
82. Poovaiah BW, Reddy ASN. Calcium and signal transcuction in plants. Crit Rev Plant Sci, 12:185-211.
83. Minorsky PV.A heuristic hypothesis of chilling injury in plants: a role for caleiumas the primary physiological transducer of injury. Plant Cell Environ, 1985,8:75-94.
84. Wang H(王红), Jian LC(简令成)。Changes of the level of Ca~(2+). in the cells of rice seedlings under low temperature stress. Acta Bot Sin(植物学报), 36:587-591.
85. Bush DS. Calcium regulation in plants, cells and its role in signaling. Annu Rev Plant Physiol Plant Mol Biol, 46:95-122.
86. Jian LC, Li JH, Chen WP, Li PH, Ahlstrand GG. Cytochemical localization of calcium and Ca~(2+)-ATPase activity under chilling stress:a eompative study between the chilling sensitive maize and the chilling insensitive winter wheat. Plant Cell Physio1,1999,40:1061-1071.
87. Jian LC(简令成)。The brief review about the topic "The cytological study on the mechanism of chilling resistant in plant" in past forty years. Chin bull Bot(植物学通报), 16(Suppl): 15-19.
88. Perez Prat E, Narasimhan ML, Binzel ML, Botella MA, Chen Z, Valpusta V, Bressan RA, Hasegawa PM. Induction of a putative Ca~(2+)-ATPase mRNA in NaCl-adapted
cells. Plant Physiol, 1992, 100: 1471-1478.
89. Zeng SX(曾韶西), LI MR. (李美如). Changes of Ca~(2+)-ATPase activities in cell of rice seedling during the enhancement of chilling resistance induced by cold and salt pretreatment. Acta Bot Sin(植物学报), 1999,41:156-160.
90. Li MR (李美如), et al. Effect of calcium on the cold-resistance of rice seedlings. Acta Phytophysiol Sin (植物生理学报), 1996, 22: 379-384.
91．林善枝等．毛白杨幼苗低温锻炼过程中Ca~(2+)的作用及细胞Ca~(2+)-ATP酶活性的变化．植物生理与分子生物学学报．2002，28(6)：449-456．
92. Li MR (李美如), et al. Antifreezing substances in plant cell and relation to cold resistance. Plant Physiol Commun, 1995, 31(5):328-334.
93. Tawfik AA, Palta J. Evidence for the effect of in vivo calcium concentration on plasma membrane ATPase activity. Plant Physiol, 1993, 102(1): 108-120.
94. Monroy A, Sarhan F, Dhindsa R. Cold-induced changes in freezing tolerance, protein phosphorylation and gene expresion. Evidence for a rode of calcium. Plant Physiol, 1993,102:1227-1234.
95. Rachael LN, Robert WW, Raymond LR. Eukaryotic DNA fragments which act as Promoters for a plasmid gene [J]. Nature, 979, 77: 24-325.
96．徐国洲等。酵母 K.cicerisporus基因组中有启动子功能的DNA片段的克隆。生物化学与生物物理学报，1998，30(1)：35-40。
97．梁明山等。海甘蓝基因启动子的分离和鉴定。中国油料作物学报，1998，20：1-6。
98．徐恒等。大肠杆菌-分枝杆菌启动子探针型穿梭载体pEQ3的构建。四川大学学报，1997，34(3)：349-353。
99．A azaz Abderranhmane等。一种具有高活性的酪氨酸酶基因启动子片段的分离。武汉大学学报，1999，45(2)：233-237。
100．张宪银等。水稻胚乳特异性启动子Gt1的克隆及其功能验证。作物学报，2002，28(1)：110-114。
101．马志刚等。马铃薯prp1-1启动子的克隆及转基因研究。西南农业学报，2002，15(1)：12-14。
102．罗克明等。棉花Lea蛋白D-113基因启动子的克隆及序列分析。遗传学报，29(2)：161-165。
103．陆海等。银杏木质部特异定位表达基因启动子克隆。北京林业大学学报。
104．李永春等。蔗糖合酶基因启动子克隆及其转基因水稻植物中的特异表达。作物学报，2002，28(5)：586-590。
105．刘复权等。烟草NFL1基因启动子区的克隆和初步的功能分析。北京大学学报(自然科学版)，2000，36(2)：263-267。
106．王新力等。香蕉果实成熟相关基因ACO1启动子区的克隆及其功能初探。生物工程学报，2001，17(4)：428-431。
107. Siebert P D. Chenchik A, Kellogg D E et al. An improved PeR metherod for walking in uncloned genomic
108．邓晓东。华南热带农业大学博士学位论文，2002。
109. Kajiwara S, Shishido K. Characterization of thepromoter region of the basidiomycete Lentunes edodes Le. ras gene. FEMS Microbiology Letters, 1992,92 (2): 147-150.
110. Hirano T, Sato T, Okawa K, et al. Isolation and characterization of the glyceraldehydes-3-phosphate dehydrogenase gene of Lentinus edodes. Bioscience biotechnology and Biochemistry, 1999, 63(7): 1223-1227.
111. Kingsnorth CS, Eastwood, DC, Burton KS. Cloning and postharvest expression of serine proteinase transcripts in the cultivated mushroom Agaricus bisporus. Fungal Genet. Biol, 2001,32(3):135-144.
112. Stark MJ, Milner JS. Cloning and analysis of the Kluyveromyces lactis TRP1 gene: a chromosomal locus flanded by genes encoding inorganic pyrophosphatase and histone H3. Yeast, 1989m5(1): 35-50.
113. Skrzynia C, Binninger DM, Pukkila PJ, et al. Molecular characterization of TRP1, a gene coding for tryptophan synthetase in the basidiomycete Coprinus cinereus. Gene, 1989, 81(1): 73-82.
114. Raguz S, Yag E, Wood, DA, et al. Isolation and characterization of a cellulose-growth-specific gene from Agaricus bisporus. Gene, 1992, 119(2): 183-190.
115. Yague E, ZunicMM, Morgan L, et al. Expression of eEL 2 and CEL4, two proteins from Agaricus bisporus with similarity to fungal cellobiohydrolase I and
beta-mannanase, respectively, is regulated by the carbon source. Microbiology, 1997, 143: 239-244.
116. Susumu Kajiwara, Kazuyoshi Yamaka, et al. Isolation and sepuence of a developmentally regulated putative novel gene, priA, from the basidiomycete Lentinus edodes. Gene, 1992, 114: 173-178.
117. Zhao J, Chen YH, Kwan HS. Molecular cloning, characterization and differential expression of a glucoamylase gene from the basidiomycetous fungus Lentunus edodes. Applied and Environmental Microbiology, 2000: 2531-2535.
118. Barbacid, M. Annu. Rev. Biochem, 1987(6): 779-827.
119. Schuncn MJ, Peter JP, Maria AP, et al. Function elements in the promoter region of the Aspergillus nidulans gpdA gene encoding plyceraldehydes-3-phosphate dehydrogenase. Gene, 1990,93:101-109.
120. Chen X, Stone M, Schlagnhaufer, et al. A fruiting body tissue method for efficient Agrobacterium-mediated transformation of Agaricus bisporus. Applied and Environmental Microbiology, 2000:4510-4513.
121. Hori, K, Kajiwara S, Saito T, et al. Cloning, sequence analysis and transcriptional expression of aras gene of the edible basidiomycete Lentinus edodes. Gene, 1991, 105(1): 91-96.
122. Osamu Ishibashi, Kazuo Shishido. Nucleaotide sequence of aras gene from the basidiomycete coprinus cinereus. Gene, 1993, 125(2): 233-234.
123. Nagase, T, Ueno, Y, and Ishii, S. Gene, 1990, 94: 249-253.
124. Yamanoto, F, and Perucho, M. Oncogene Res, 1988,3:125-138.
125. Hoffman, E. K, Trusko, S.P, Freeman, N, and George, D.L. Mol, Cell, Biol, 1987(7): 2592-2596.
126. Hall, A. and Brown, R. Nucleic Acids Res, 1985, 13: 5255-5268.
127. Kadonaga, J.T., Carner, K.R., Masiarz, F.R. and Tjian, R. Cell, 1987, 51: 1079-1090.
128. Beeathnach, R. and Chambon, P. Annu. Res. Biochem. 1981,50:249-383.
129. Myers, R.M.,Tilly, k. and Maniatis, T. Science, 1986,232:613-618.
130. Bishop, J.G and Corces, V.G. Gene Der, 1988,2:567-577.
131. Sato T, Yaegashi K, Ishll S, et al. Transformation of the edible basidiomycete Lentinus edodes by restriction enzyme-mediated DNA integration of plasmid DNA. Bioteehnol. Biochem, 1998, 62(12): 2346-2358.
132. Yanai K, Yonekura K, Usami H, et al. The integrative transformationof Pleurotus ostreatus using Biolaphos resistance as a dominant selectable marder. Bioscience Biotechnology Biochemistry, 1996, 60(3): 472-475.
133. Granado JD, Kertesz-Chaloupkova, Aebi M, et al. Restriction enzyme-mediate DNA integration in Coprinus cinereus. Mol. Gen. Genet, 1997, 256: 28-36.