雪峰乌骨鸡蛋氨酸需要量的研究
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摘要
选用1日龄雪峰乌骨鸡490羽，随机分成五组，每组五个重复，其中两个重复进行网上平养，每个重复40羽，另三个重复进行笼养，每个重复6羽。在满足其它营养素的条件下，基础饲粮中分别添加合成蛋氨酸0.00％、0.06％、0.12％、0.18％、0.24％，配合成五组饲粮，分别饲养雪峰乌骨鸡，采取自由采食和饮水。在试验期的4周龄、8周龄分别对试鸡生产性能、肌肉沉积率、血清尿素氮含量、血清氨基酸含量测定，同时8周龄进行屠宰测定，其结果：
(1) 0—4周龄，累计生长：添加蛋氨酸0.12％组和0.24％组分别显著高于对照组(p＜0.05)，0.18％组极显著高于对照组(p＜0.01)。相对生长：0.12％组、0.18％组分别极显著高于对照组(p＜0.01)，0.24％组与对照组差异显著(p＜0.05)。日增重：0.12％组、0.24％组分别显著高于对照组(p＜0.05)，0.18％组极显著高于对照组(p＜0.01)。饲料效率：0.12％组和0.18％组分别与对照组差异显著(p＜0.05)，0.18％组与0.06％组差异显著(p＜0.05)。胸肌沉积率：0.18％组分别显著高于对照组及0.06％组(p＜0.05)。腿肌沉积率：0.12％组、0.18％组、0.24％组分别显著高于对照组(p＜0.05)，0.06％组极显著高于对照组(p＜0.01)。血清尿素氮含量以0.24％组最低，血清氨基酸：血清蛋氨酸含量、天冬氨酸含量、谷氨酸含量均以0.06％组最低。
(2) 5—8周龄，累计生长：0.12％组显著高于对照组(p＜0.05)，0.18％组极显著高于对照组(p＜0.01)。相对生长率：0.12％组和0.18％组分别显著高于对照组(p＜0.05)。日增重：0.06％组、0.12％组分别显著高于对照组(p＜0.05)，0.18％组极显著高于对照组(p＜0.01)。饲料效率：0.12％组与对照组差异显著(p＜0.05)。屠宰产量：0.12％组显著高于对照组(p＜0.05)。胸肌沉积率和腿肌沉积率：0.12％组、0.18％组分别显著高于对照组(p＜0.05)。腹脂沉积率：0.12％组显著低于对照组(p＜0.05)。胸肌系水力：各组之间差异不显著(p＞0.05)。血清尿素氮含量以0.18％组最低，并与对照组差异显著(p＜0.05)。血清氨基酸水平：血清蛋氨酸含量以0.18％最低，血清天冬氨酸含量以0.12％组为最低，血清谷氨酸含量以0.06％组为最低。
(3) 对各个时期所测指标进行相关和回归分析，0—4周龄：饲粮蛋氨酸的水平与生
长性能、饲料效率及血清天冬氨酸含量之间存在相关性，并有显著的二次曲线关系。5一8
周龄:饲粮蛋氨酸水平与生长性能及肌肉沉积率存在相关性，并有显著的二次曲线关系。
因此，通过综合考虑各项指标，0一4周龄在代谢能为12.32MJ/Kg、粗蛋白质为21%
的营养水平下，建议雪峰乌骨鸡蛋氨酸需要量为0.48%。5一8周龄在代谢能为
11.92MJ/Kg、粗蛋白质为19，4%的营养水平，建议雪峰乌骨鸡蛋氨酸需要量为0.42%。
A total of 490 Xue-Feng black-bone chickens were assigned to 5 treatments at random with 5 replicates of each treatment ,among them 2 replicates were fed on net, each replicate has 40 chickens, 3 replicates were fed in cage, each cage fed 6 chickens. Meeting the other nutrients requirement other than methionine ,the diets supplemented with diffetent level of methionine (0.00%, 0.06%, 0.12%, 0.18%,0.24%) were assigned to birds . It was access to feed and water, performance, deposition of muscles, serum urea nitrogen ,serum amino acid were examined at the 4th and 8th weeks respectively and dressing traits were examined at the 8th week. The main results were as follow:
(1) During 0~4 weeks, accumulative growth: 0.12% group and 0.24% group were significantly higher than control group (p<0.05). 0.18% group was very significantly higher than control group (p<0.01). Relative growth ratio: 0.12% group and 0.18% group were very significantly higher than control group (p<0.01), there was significantly difference between 0.24% group and control group (p<0.05).Daily gain: 0.12% group and 0.24% group were significantly higher than control group(p<0.05),0.18% group was very siginificantly higher than control group(p<0.01). Feed efficiency: there were significantly different between 012% group or 0.18% group and control group, between 0.18% group and 0.06% group (p<0.05). Deposition ratio of chest muscles: 0.18% group was significantly higher than control group and 0.06% group(p<0.05). Deposition ratio of leg muscles: 0.12% group and 0.18% group and 0.24% group were significantly higher than control group(p<0.05), 0.06% group was very significantly higher than control group (p<0.01). 0.24%group was the lowerst in serum urea nitrogen. 0.06% group was the lowst in serum urea nitrogen and serum methionine and serum aspartic acid and serum glutamic acid.
(2) During 5~8 weeks, accumulative growth: 0.12% group was significantly higher than control group(p<0.05),0.18% group was very significantly higher than control group (p<0.01). Reletive growth ratio: 0.12% group and 0.18% group were significantly higher than control group
(p<0.05). Daily gain: 0.06% group and 0.12% group were significantly higher than control group (p<0.05), 0.18% group was very significantly higher than control group(p<0.01). Feed efficiency: There was significantly different between 0.12% group and control group(p<0.05). Butcher the output: 0.12% group was significantly higher than control group (p<0.05). Deposition ratio of chest muscles and leg muscles: 0.12% group and 0.18% group were significantly higher than control group(p<0.05). The fat depositing ratio of belly: 0.12% group was significantly lower than control group. Water conservancy ratio in chest muscles: It was not significantly different amang each groups. 0.18% group was the lowest in serum urea nitrogen, it was significantly different between 0.18% group and control group(p<0.05). Serum amino acid: 0.18% group was lowest in serum methionine, 0.12% group was lowest in serum aspartic acid . 0.06% was lowest in serum glutamic acid.
(3) Regression analysis was conducted between investigated parameters and dietary methionine levels. 0~4 weeks, there was significantly quadric relation between the different level of methionine of the diets and performance or feed efficiency or serum aspartic acid. 5~8 weeks, it was relativity and significantly conic relation between the different level of methionine of the diets and performance or deposition ratio of muscles.
Based on the above results and ME: 12.32MJ/Kg and CP: 21%, it is suggested that optimum methionine level in Xue-Feng black-bone chickens is 0.48% for 0~ 4 weeks birds. Based on the above results and ME: 11.92MJ/Kg and CP: 19.4% , it is suggested that optimum methionine level in Xue-Feng black-bone chickens is 0.42% for 5~8 weeks birds.
(1) 0—4周龄，累计生长：添加蛋氨酸0.12％组和0.24％组分别显著高于对照组(p＜0.05)，0.18％组极显著高于对照组(p＜0.01)。相对生长：0.12％组、0.18％组分别极显著高于对照组(p＜0.01)，0.24％组与对照组差异显著(p＜0.05)。日增重：0.12％组、0.24％组分别显著高于对照组(p＜0.05)，0.18％组极显著高于对照组(p＜0.01)。饲料效率：0.12％组和0.18％组分别与对照组差异显著(p＜0.05)，0.18％组与0.06％组差异显著(p＜0.05)。胸肌沉积率：0.18％组分别显著高于对照组及0.06％组(p＜0.05)。腿肌沉积率：0.12％组、0.18％组、0.24％组分别显著高于对照组(p＜0.05)，0.06％组极显著高于对照组(p＜0.01)。血清尿素氮含量以0.24％组最低，血清氨基酸：血清蛋氨酸含量、天冬氨酸含量、谷氨酸含量均以0.06％组最低。
(2) 5—8周龄，累计生长：0.12％组显著高于对照组(p＜0.05)，0.18％组极显著高于对照组(p＜0.01)。相对生长率：0.12％组和0.18％组分别显著高于对照组(p＜0.05)。日增重：0.06％组、0.12％组分别显著高于对照组(p＜0.05)，0.18％组极显著高于对照组(p＜0.01)。饲料效率：0.12％组与对照组差异显著(p＜0.05)。屠宰产量：0.12％组显著高于对照组(p＜0.05)。胸肌沉积率和腿肌沉积率：0.12％组、0.18％组分别显著高于对照组(p＜0.05)。腹脂沉积率：0.12％组显著低于对照组(p＜0.05)。胸肌系水力：各组之间差异不显著(p＞0.05)。血清尿素氮含量以0.18％组最低，并与对照组差异显著(p＜0.05)。血清氨基酸水平：血清蛋氨酸含量以0.18％最低，血清天冬氨酸含量以0.12％组为最低，血清谷氨酸含量以0.06％组为最低。
(3) 对各个时期所测指标进行相关和回归分析，0—4周龄：饲粮蛋氨酸的水平与生
长性能、饲料效率及血清天冬氨酸含量之间存在相关性，并有显著的二次曲线关系。5一8
周龄:饲粮蛋氨酸水平与生长性能及肌肉沉积率存在相关性，并有显著的二次曲线关系。
因此，通过综合考虑各项指标，0一4周龄在代谢能为12.32MJ/Kg、粗蛋白质为21%
的营养水平下，建议雪峰乌骨鸡蛋氨酸需要量为0.48%。5一8周龄在代谢能为
11.92MJ/Kg、粗蛋白质为19，4%的营养水平，建议雪峰乌骨鸡蛋氨酸需要量为0.42%。
A total of 490 Xue-Feng black-bone chickens were assigned to 5 treatments at random with 5 replicates of each treatment ,among them 2 replicates were fed on net, each replicate has 40 chickens, 3 replicates were fed in cage, each cage fed 6 chickens. Meeting the other nutrients requirement other than methionine ,the diets supplemented with diffetent level of methionine (0.00%, 0.06%, 0.12%, 0.18%,0.24%) were assigned to birds . It was access to feed and water, performance, deposition of muscles, serum urea nitrogen ,serum amino acid were examined at the 4th and 8th weeks respectively and dressing traits were examined at the 8th week. The main results were as follow:
(1) During 0~4 weeks, accumulative growth: 0.12% group and 0.24% group were significantly higher than control group (p<0.05). 0.18% group was very significantly higher than control group (p<0.01). Relative growth ratio: 0.12% group and 0.18% group were very significantly higher than control group (p<0.01), there was significantly difference between 0.24% group and control group (p<0.05).Daily gain: 0.12% group and 0.24% group were significantly higher than control group(p<0.05),0.18% group was very siginificantly higher than control group(p<0.01). Feed efficiency: there were significantly different between 012% group or 0.18% group and control group, between 0.18% group and 0.06% group (p<0.05). Deposition ratio of chest muscles: 0.18% group was significantly higher than control group and 0.06% group(p<0.05). Deposition ratio of leg muscles: 0.12% group and 0.18% group and 0.24% group were significantly higher than control group(p<0.05), 0.06% group was very significantly higher than control group (p<0.01). 0.24%group was the lowerst in serum urea nitrogen. 0.06% group was the lowst in serum urea nitrogen and serum methionine and serum aspartic acid and serum glutamic acid.
(2) During 5~8 weeks, accumulative growth: 0.12% group was significantly higher than control group(p<0.05),0.18% group was very significantly higher than control group (p<0.01). Reletive growth ratio: 0.12% group and 0.18% group were significantly higher than control group
(p<0.05). Daily gain: 0.06% group and 0.12% group were significantly higher than control group (p<0.05), 0.18% group was very significantly higher than control group(p<0.01). Feed efficiency: There was significantly different between 0.12% group and control group(p<0.05). Butcher the output: 0.12% group was significantly higher than control group (p<0.05). Deposition ratio of chest muscles and leg muscles: 0.12% group and 0.18% group were significantly higher than control group(p<0.05). The fat depositing ratio of belly: 0.12% group was significantly lower than control group. Water conservancy ratio in chest muscles: It was not significantly different amang each groups. 0.18% group was the lowest in serum urea nitrogen, it was significantly different between 0.18% group and control group(p<0.05). Serum amino acid: 0.18% group was lowest in serum methionine, 0.12% group was lowest in serum aspartic acid . 0.06% was lowest in serum glutamic acid.
(3) Regression analysis was conducted between investigated parameters and dietary methionine levels. 0~4 weeks, there was significantly quadric relation between the different level of methionine of the diets and performance or feed efficiency or serum aspartic acid. 5~8 weeks, it was relativity and significantly conic relation between the different level of methionine of the diets and performance or deposition ratio of muscles.
Based on the above results and ME: 12.32MJ/Kg and CP: 21%, it is suggested that optimum methionine level in Xue-Feng black-bone chickens is 0.48% for 0~ 4 weeks birds. Based on the above results and ME: 11.92MJ/Kg and CP: 19.4% , it is suggested that optimum methionine level in Xue-Feng black-bone chickens is 0.42% for 5~8 weeks birds.
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2、赵英复．开发乌鸡的建议[J]．吉林畜牧兽医，1990，(3)：20-21．
3、郭万红，程序芳，曾庆玲．泰和乌骨鸡适宜营养水平初步研究[J]．中国家禽，1996，(7)：38．
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