吉林白鹅IGF-I基因的SNP与产绒性状的相关性研究
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摘要
类胰岛素生长因子-Ⅰ(Insulin-Like Growth factor-Ⅰ，IGF-Ⅰ)是一种对动物生长、发育、繁殖、营养代谢以及组织细胞的增殖、分化、凋亡和癌变有直接影响的多功能生物活性肽。本研究设计了5对引物，对吉林白鹅IGF-Ⅰ基因进行PCR扩增，并采用SSCP和测序的方法检测了吉林白鹅类胰岛素样生长因子-Ⅰ不同的基因片段的多态性，对单核苷酸多态性进行了群体遗传学分析，探索不同基因型与鹅产绒性能之间的关系。
利用PCR-SSCP方法，在吉林白鹅IGF-Ⅰ基因引物303的PCR扩增产物结果中检测到从、BB、AB三种基因型，结果表明，A等位基因频率为0.5219，B等位基因频率为0.4781。群体基因频率和基因型频率分布之间差异显著(P＜0.01)，处于非Hardy-Weinberg平衡状态。
测序结果表明，引物303在产物第93bp碱基处存在一个点突变C→T。
相关性统计分析结果表明，引物303的AA基因型与吉林白鹅的背部伞形羽羽毛长度和胸部成年绒羽羽毛长度呈正相关，AB基因型与吉林白鹅的背部伞形羽羽毛长度和胸部成年绒羽羽毛长度呈负相关。
本试验通过对吉林白鹅羽毛生长规律的观察，并且通过对IGF-Ⅰ基因在雏绒羽、伞形羽和成年绒羽的背部、腹部和胸部的直径、羽毛长度和羽丝长度中的相关性分析研究，在IGF-Ⅰ基因的外显子1内找到了1处SNP，并发现该突变与背部伞形羽长度和胸部成年绒羽长度相关，为进一步了解羽毛生长发育调控的分子机制提供了一些基础。
IGF-I is a kind of bioactive peptide and it has direct influence on the growth, development, reproduction and nutritious metabolism of the organism, and it is also influences the proliferation, differentiation, apoptosis and carcinomatous change of the cells directly. 5 pairs of primers were designed in this experiment to amplify the IGF-I gene of Jilin White Goose, and SSCP technique was used to detect the polymorphism existed in the different fragments of IGF-1 gene. The SNPs in the test population was analyzed to investigate the relationship between the different genetypes and down traits.
The results of PCR-SSCP showed that three genotypes (AA,BB,AB) were detected in primer303 .The gene frequencies for allele A and B were 0.5219 and 0.4781 in primer303.The chi-sequare test (X~2) showed the distribution of genotype of the primer 303 was significant different (P＜0.01) among population,and this SNP loci was not in Hardy-Weinberg Equilibrium status (HWE).
Sequencing results indicated that a mutation were detected in primer303.There were 93bp nucleotide site (C-T) in sequencing results.
Statistical analyses of correlation showed that the genotype AA of the primer 303 was positive correlation to the length of the unfledged quill coverts on back and the length of the the grown quill-coverts on brisket,while genotype AB was negatively related.The result showed there are no difference for other characters (P＜0.01).
利用PCR-SSCP方法，在吉林白鹅IGF-Ⅰ基因引物303的PCR扩增产物结果中检测到从、BB、AB三种基因型，结果表明，A等位基因频率为0.5219，B等位基因频率为0.4781。群体基因频率和基因型频率分布之间差异显著(P＜0.01)，处于非Hardy-Weinberg平衡状态。
测序结果表明，引物303在产物第93bp碱基处存在一个点突变C→T。
相关性统计分析结果表明，引物303的AA基因型与吉林白鹅的背部伞形羽羽毛长度和胸部成年绒羽羽毛长度呈正相关，AB基因型与吉林白鹅的背部伞形羽羽毛长度和胸部成年绒羽羽毛长度呈负相关。
本试验通过对吉林白鹅羽毛生长规律的观察，并且通过对IGF-Ⅰ基因在雏绒羽、伞形羽和成年绒羽的背部、腹部和胸部的直径、羽毛长度和羽丝长度中的相关性分析研究，在IGF-Ⅰ基因的外显子1内找到了1处SNP，并发现该突变与背部伞形羽长度和胸部成年绒羽长度相关，为进一步了解羽毛生长发育调控的分子机制提供了一些基础。
IGF-I is a kind of bioactive peptide and it has direct influence on the growth, development, reproduction and nutritious metabolism of the organism, and it is also influences the proliferation, differentiation, apoptosis and carcinomatous change of the cells directly. 5 pairs of primers were designed in this experiment to amplify the IGF-I gene of Jilin White Goose, and SSCP technique was used to detect the polymorphism existed in the different fragments of IGF-1 gene. The SNPs in the test population was analyzed to investigate the relationship between the different genetypes and down traits.
The results of PCR-SSCP showed that three genotypes (AA,BB,AB) were detected in primer303 .The gene frequencies for allele A and B were 0.5219 and 0.4781 in primer303.The chi-sequare test (X~2) showed the distribution of genotype of the primer 303 was significant different (P＜0.01) among population,and this SNP loci was not in Hardy-Weinberg Equilibrium status (HWE).
Sequencing results indicated that a mutation were detected in primer303.There were 93bp nucleotide site (C-T) in sequencing results.
Statistical analyses of correlation showed that the genotype AA of the primer 303 was positive correlation to the length of the unfledged quill coverts on back and the length of the the grown quill-coverts on brisket,while genotype AB was negatively related.The result showed there are no difference for other characters (P＜0.01).
引文
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