运动对肥胖大鼠脂类代谢调节因素的影响
摘要
目的:研究高脂饮食对大鼠、一次性运动和长期运动对肥胖大鼠脂肪组织HSL活性、蛋白及基因表达,perilipin蛋白和基因表达,PPAR-γ基因表达以及血脂、体重等的影响。
方法:将116只3周龄SD雄性大鼠随机分成高脂组(n=110)和普食组(C,n=6),10周后,形成66只肥胖鼠(OB组)和44只肥胖抵抗鼠(OR组)。将OB鼠随机分为:对照组(EC)、一次性运动即刻(E0)、1h(E1)、3h(E3)、12h(E12)、24h(E24);长期对照(LC)和长期运动组(LE)。一次性运动,跑台速度为20m/min,坡度50;LE组每天进行40 min同样强度运动8周。用化学法测试大鼠血脂、HSL活性等;用Western Blotting法测试脂肪组织HSL和perilipin蛋白表达;用Real-Time PCR法测试脂肪组织HSL、perilipin和PPAR-γ基因表达。
结果:
1、10周后,OB组大鼠体重、血脂等明显高于C组;而OB组脂肪组织HSL活性、蛋白和基因表达显著低于C组;OB组perilipin蛋白和基因表达显著高于C组,但显著低于OR组;OB组PPAR-γ基因表达显著高于C组。
2、与EC组相比,一次性运动后大鼠血清FFA和甘油、脂肪组织HSL活性、蛋白和基因表达出现不同程度的升高;而perilipin蛋白表达除即刻出现显著升高外,其它各时间点均显著降低;perilipin和PPAR-γ基因表达均出现不同程度的降低。
3、与LC组相比,LE组大鼠体重、血清FFA和甘油均显著下降;HSL活性显著升高;HSL蛋白表达有降低趋势;perilipin蛋白表达及HSL、perilipin和PPAR-γ基因表达显著减少。
结论:
1、高脂饮食导致大鼠肥胖,可能与其脂肪组织PPAR-γ升高,进而上调perilipin基因和蛋白表达,导致大鼠体内脂肪储存和肥胖;还可能与其HSL活性、蛋白和基因表达下调有关。
2、一次性运动导致肥胖大鼠脂解增强,可能与其脂肪组织HSL活性、蛋白和基因表达上调及PPAR-γ基因表达下调有关。
3、长期运动可减轻肥胖大鼠体重,可能与其脂肪组织HSL活性增加、perilipin蛋白和基因表达以及perilipin调节因子PPAR-γ基因表达下调有关。
Aims:
To research the effects of 10-week high-fat diet on rats as well as a single bout of exercise and long-term exercise on obese rats adipose tissue hormone sensitive lipase(HSL) activity,protein and gene expression;perilipin protein and gene expression;peroxisome proliferator activated receptor-γ(PPAR-γ) gene expression as well as blood fat,weight et al.
Methods:
116 3-week-age Sprange-Dawley male rats were randomly divided into high-fat group(n=110)and general diet control group(C,n=6).After 10 weeks,66 obese rats(OB group) and 44 obesity resistant rats(OR group) were differentiated.Then the OB rats were randomly divided into: non-exercise control group(EC),immediate post-exercise group(E0) as well as 1(E1),3(E3),12(E12),and 24(E24) hours after exercise group; long-term control group(LC) and long-term exercise group(LE).The concentrations of blood fat,blood sugar,serum free fatty acids(FFA), serum glycerol,adipose tissue HSL activity of obese rats were tested using chemical method.Then the HSL and perilipin protein expressions of rats adipose tissue were tested with Western Blotting method,and the HSL,perilipin and PPAR-γgene expressions of rats adipose tissue were tested with Real-Time PCR method.
Results:
1.After 10-week high-fat diet,the weight,fat weight,serum FFA and serum glycerol of obesity group(OB group) rats were significantly higher than general diet control group(C group) and/or obesity resistance group (OR group);OB group rats' food availability was higher than OR group or C group(P<0.01).While,the OB group rats adipose tissue HSL activity, HSL protein content as well as gene expression were markedly lower than C group(P＜0.05).And the OB group rats adipose tissue perilipin protein expression as well gene expression were markedly higher than C group (P＜0.05),but significantly lower than OR group(P＜0.01).The OB group PPAR-γgene expression was markedly higher than C group(P＜0.01).
2.Compared with the non-exercise control group(EC group),after a single bout of exercise,the serum FFA and serum glycerol concentrations, adipose tissue HSL activity,HSL protein and gene expression were markedly elevated at different times.However,the adipose tissue perilipin expression was only higher immediately after a single bout of exercise,and markedly lower at other times.The perilipin and PPAR-γgene expression declined significantly at different times,too.
3.Compared with the long-term control group(LC group),the weight,serum FFA and serum glycerol concentrations of long-term exercise group(LE group) reduced markedly.The LE group adipose tissue HSL activity markedly rose(P＜0.01);however,the LE group adipose tissue HSL protein expression tended to decline,and the adipose tissue perilipin protein expression(P＜0.01),HSL(P＜0.05),perilipin(P＜0.05)and PPAR-γgene expressions(P＜0.05) reduced markedly.
Conclusions:
1.10-week high fat diet result in rats to become obese and dyslipidemia, this may be related to the adipose tissue PPAR-γgene expression elevation to upregulate perilipin gene expression and perilipin protein expression,since perilipin may induce fat storage and obesity;on the other hand,the downregulation of rats adipose tissue HSL activity,HSL protein expression as well as HSL gene expression were also related to the bad results.
2.A single bout of exercise may result in the increment of obese rats lipolysis during and after exercise.And the mechanisms of lipolysis rising may be involved in the upregulation of its adipose tissue HSL activity,HSL protein expression,HSL gene expression as well as the downregulation of PPAR-γgene expression.
3.Long-term exercise may reduce the rats weight,and these decline could have relation with the HSL activity increment as well as perilipin protein level and gene level decline,and had relation with the downregulation of the perilipin regulatory factor PPAR-γgene expression,too.
方法:将116只3周龄SD雄性大鼠随机分成高脂组(n=110)和普食组(C,n=6),10周后,形成66只肥胖鼠(OB组)和44只肥胖抵抗鼠(OR组)。将OB鼠随机分为:对照组(EC)、一次性运动即刻(E0)、1h(E1)、3h(E3)、12h(E12)、24h(E24);长期对照(LC)和长期运动组(LE)。一次性运动,跑台速度为20m/min,坡度50;LE组每天进行40 min同样强度运动8周。用化学法测试大鼠血脂、HSL活性等;用Western Blotting法测试脂肪组织HSL和perilipin蛋白表达;用Real-Time PCR法测试脂肪组织HSL、perilipin和PPAR-γ基因表达。
结果:
1、10周后,OB组大鼠体重、血脂等明显高于C组;而OB组脂肪组织HSL活性、蛋白和基因表达显著低于C组;OB组perilipin蛋白和基因表达显著高于C组,但显著低于OR组;OB组PPAR-γ基因表达显著高于C组。
2、与EC组相比,一次性运动后大鼠血清FFA和甘油、脂肪组织HSL活性、蛋白和基因表达出现不同程度的升高;而perilipin蛋白表达除即刻出现显著升高外,其它各时间点均显著降低;perilipin和PPAR-γ基因表达均出现不同程度的降低。
3、与LC组相比,LE组大鼠体重、血清FFA和甘油均显著下降;HSL活性显著升高;HSL蛋白表达有降低趋势;perilipin蛋白表达及HSL、perilipin和PPAR-γ基因表达显著减少。
结论:
1、高脂饮食导致大鼠肥胖,可能与其脂肪组织PPAR-γ升高,进而上调perilipin基因和蛋白表达,导致大鼠体内脂肪储存和肥胖;还可能与其HSL活性、蛋白和基因表达下调有关。
2、一次性运动导致肥胖大鼠脂解增强,可能与其脂肪组织HSL活性、蛋白和基因表达上调及PPAR-γ基因表达下调有关。
3、长期运动可减轻肥胖大鼠体重,可能与其脂肪组织HSL活性增加、perilipin蛋白和基因表达以及perilipin调节因子PPAR-γ基因表达下调有关。
Aims:
To research the effects of 10-week high-fat diet on rats as well as a single bout of exercise and long-term exercise on obese rats adipose tissue hormone sensitive lipase(HSL) activity,protein and gene expression;perilipin protein and gene expression;peroxisome proliferator activated receptor-γ(PPAR-γ) gene expression as well as blood fat,weight et al.
Methods:
116 3-week-age Sprange-Dawley male rats were randomly divided into high-fat group(n=110)and general diet control group(C,n=6).After 10 weeks,66 obese rats(OB group) and 44 obesity resistant rats(OR group) were differentiated.Then the OB rats were randomly divided into: non-exercise control group(EC),immediate post-exercise group(E0) as well as 1(E1),3(E3),12(E12),and 24(E24) hours after exercise group; long-term control group(LC) and long-term exercise group(LE).The concentrations of blood fat,blood sugar,serum free fatty acids(FFA), serum glycerol,adipose tissue HSL activity of obese rats were tested using chemical method.Then the HSL and perilipin protein expressions of rats adipose tissue were tested with Western Blotting method,and the HSL,perilipin and PPAR-γgene expressions of rats adipose tissue were tested with Real-Time PCR method.
Results:
1.After 10-week high-fat diet,the weight,fat weight,serum FFA and serum glycerol of obesity group(OB group) rats were significantly higher than general diet control group(C group) and/or obesity resistance group (OR group);OB group rats' food availability was higher than OR group or C group(P<0.01).While,the OB group rats adipose tissue HSL activity, HSL protein content as well as gene expression were markedly lower than C group(P＜0.05).And the OB group rats adipose tissue perilipin protein expression as well gene expression were markedly higher than C group (P＜0.05),but significantly lower than OR group(P＜0.01).The OB group PPAR-γgene expression was markedly higher than C group(P＜0.01).
2.Compared with the non-exercise control group(EC group),after a single bout of exercise,the serum FFA and serum glycerol concentrations, adipose tissue HSL activity,HSL protein and gene expression were markedly elevated at different times.However,the adipose tissue perilipin expression was only higher immediately after a single bout of exercise,and markedly lower at other times.The perilipin and PPAR-γgene expression declined significantly at different times,too.
3.Compared with the long-term control group(LC group),the weight,serum FFA and serum glycerol concentrations of long-term exercise group(LE group) reduced markedly.The LE group adipose tissue HSL activity markedly rose(P＜0.01);however,the LE group adipose tissue HSL protein expression tended to decline,and the adipose tissue perilipin protein expression(P＜0.01),HSL(P＜0.05),perilipin(P＜0.05)and PPAR-γgene expressions(P＜0.05) reduced markedly.
Conclusions:
1.10-week high fat diet result in rats to become obese and dyslipidemia, this may be related to the adipose tissue PPAR-γgene expression elevation to upregulate perilipin gene expression and perilipin protein expression,since perilipin may induce fat storage and obesity;on the other hand,the downregulation of rats adipose tissue HSL activity,HSL protein expression as well as HSL gene expression were also related to the bad results.
2.A single bout of exercise may result in the increment of obese rats lipolysis during and after exercise.And the mechanisms of lipolysis rising may be involved in the upregulation of its adipose tissue HSL activity,HSL protein expression,HSL gene expression as well as the downregulation of PPAR-γgene expression.
3.Long-term exercise may reduce the rats weight,and these decline could have relation with the HSL activity increment as well as perilipin protein level and gene level decline,and had relation with the downregulation of the perilipin regulatory factor PPAR-γgene expression,too.
引文
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