基于“面口合谷收”理论的经穴体表特异性联系研究
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摘要
目的
以“面口合谷收”理论为指导,运用神经肌电检测、痛阂测定、荧光分光光度检测等方法技术,观察针刺合谷穴对恒河猴面口部肌肉运动、正常人感觉传入及唾液腺分泌的影响,证明合谷穴与面口部之间存在特异性联系的基本事实,探索经脉体表特异性联系的生物学基础,进一步丰富经穴效应特异性的科学内涵。
方法
1.针刺不同穴区对恒河猴面口部肌电的影响：按照随机数字表将恒河猴随机分为合谷穴区组、后溪穴区组、外关穴区组,分别在生理、麻醉、局麻三种不同状态下观察记录针剌前后恒河猴面口部额肌、颧肌、口轮匝肌的肌电活动,比较研究针刺合谷穴区、后溪穴区、外关穴区对恒河猴面口部肌电的不同影响。
2.针刺不同穴位对正常人面部感觉及唾液腺分泌功能的影响：运用SPSS软件编程的方法将健康受试者随机分为合谷穴组、后溪穴组、外关穴组,分别针刺健康受试者合谷穴、后溪穴、外关穴,运用钾离子测痛法观察记录针刺前后受试者面部痛阈,运用荧光分光光度法测定针刺前后受试者唾液α-淀粉酶活力,比较研究针刺合谷穴、后溪穴、外关穴对健康受试者面部痛阈及唾液α-淀粉酶活力的不同影响。
结果
1.在生理、麻醉、局麻三种不同状态下针刺不同穴区对恒河猴面口部肌电的影响：①生理状态下针刺合谷穴区对恒河猴额肌肌电的峰峰值、面积及潜伏期有明显影响(P<0.05),对颧肌肌电的峰峰值、面积及潜伏期有明显影响(P<0.01),对口轮匝肌肌电的频率、面积及潜伏期有明显影响(P<0.05)；生理状态下针刺合谷穴区对面口部肌肉肌电的影响明显强于针刺后溪穴区、外关穴区：额肌肌电频率、峰峰值、面积及潜伏期变化率,合谷穴区明显高于后溪穴区、外关穴区(P<0.05)；颧肌肌电频率、峰峰值、面积及潜伏期变化率,合谷穴区明显高于后溪穴区、外关穴区(P<0.01)；口轮匝肌肌电频率、峰峰值、面积及潜伏期变化率,合谷穴区明显高于后溪穴区、外关穴区(P<0.05)。
②麻醉状态下针刺合谷穴区对恒河猴额肌肌电的峰峰值、面积及潜伏期有明显影响(P<0.01),对其颧肌肌电的峰峰值、面积及潜伏期有明显影响(P<0.05),对其口轮匝肌肌电的频率、峰峰值、面积有明显影响(P<0.05)；麻醉状态下针刺合谷穴区对恒河猴面口部肌肉肌电的影响明显强于针刺后溪穴区、外关穴区：额肌肌电频率、峰峰值、面积及潜伏期变化率,合谷穴区明显高于后溪穴区、外关穴区(P<0.01)；颧肌肌电峰峰值、面积及潜伏期变化率,合谷穴区明显高于后溪穴区、外关穴区(P<0.01)；口轮匝肌肌电峰峰值、面积及潜伏期变化率,合谷穴区明显高于后溪穴区、外关穴区(P<0.01)。
③局麻状态下针刺合谷穴区对恒河猴额肌肌电的峰峰值、面积有明显影响(P(0.05),对其颧肌肌电的峰峰值、面积有明显影响(P<0.01)；在局麻状态下针刺合谷穴区对恒河猴面口部肌肉肌电的影响明显强于针刺后溪穴区、外关穴区：额肌肌电峰峰值、面积及潜伏期变化率,合谷穴区明显高于后溪穴区、外关穴区(P<0.05)；颧肌肌电峰峰值、面积变化率,合谷穴区明显高于后溪穴区、外关穴区(P<0.05)。
2.针刺不同穴位对正常人面部感觉的影响：针刺健康受试者合谷穴对其面口部下颌区痛阈有明显影响(P<0.05)；针刺健康受试者合谷穴对其面口部额区、颊区、下颌区痛阈变化率的影响明显强于针刺后溪穴、外关穴(P<0.05)。
3.针刺不同穴位对正常人唾液腺分泌功能的影响：针刺健康受试者合谷穴对唾液α-淀粉酶活力变化率的影响明显强于针刺后溪穴、外关穴(P<0.05)。
结论
1.在生理、全麻状态下针刺合谷穴区对恒河猴面口部的肌电活动有明显影响,其影响范围、影响程度明显大于(强于)针刺后溪穴区、外关穴区的影响,提示恒河猴合谷穴区与面口部肌肉运动之间存在相对特异性的联系。
2.局麻(穴位局部封闭)可明显减弱针刺合谷穴区对恒河猴面口部肌电活动的影响,提示外周感觉神经传入可能是实现合谷穴区与面口部联系的重要生物学基础。
3.针刺合谷穴对正常人面部痛阈、唾液α-淀粉酶活力有明显影响,其影响程度明显强于针刺后溪穴、外关穴的影响,提示正常人合谷穴与面口部感觉、唾液腺分泌功能之间存在相对特异性的联系。
Objectives
To prove the fact of the specific correlation between Hegu(LI4) and face,we used EMG detection, pain threshold detection and fluorospectrophotometry method and observed the effect of acupuncturing Hegu(LI4) on the facial muscular movement of the rhesuses, the facial sensory reception and the salivary glands function of the healthy people by "for the face and mouth,Hegu(LI4) controls"as a guide to explore the biological basis of specific correlation between meridians and the body surface and to rich the scientific content of specificity of meiridians and acupoints effect.
Method
1.The effect of acupuncturing different acupoints area of the rhesuses on the EMG of the facial muscles:We divided the rhesuses into Hegu(LI4) area,Houxi(SI3) area and Waiguan(SJ5) area groups using the random number table.We acupunctured respectively Hegu(LI4) area,Houxi(S13) area and Waiguan(SJ5) area of the rhesuses under the physiological condition and general or local anesthesia conditions to observe and record the changes of the EMG of the frontal muscles.the zygomatic muscles and the orbicular muscles of mouth in order to compare the effect of acupuncturing Hegu(LI4) area,Houxi(SI3) area and Waiguan (SJ5) area of the rhesuses on the EMG of the face and mouth.
2. The effect of acupuncturing different acupoints of the healthy people on the facial sensation and the function of salivary glands:We randomly divided the healthy people into Hegu(L14),Houxi(SI3) and Waiguan(SJ5) groups using SPSS. We acupunctured respectively Hegu(LI4),Houxi(SI3) and Waiguan(SJ5) of the healthy people to observe the changes of the facial pain threshold using the potassium-ion density dolorimeter and detect the changes of the saliva alpha-amylase activity using fluorospectrophotometry in order to compare the effect of acupuncturing Hegu(LI4),Houxi(SI3) and Waiguan(SJ5) of the healthy people on the facial pain threshold and the saliva alpha-amylase activity.
Results
l.The effect of acupuncturing respectively different acupoints area of the rhesuses on the EMG of the facial muscles under the physiological condition and local or general anesthesia conditions:①The effect of acupuncturing Hegu(LI4) area on the peak,area and incubation period changing rate of the frontal muscles of the rhesuses showed significant deviation under the physiological condition (p<0.05). The effect of acupuncturing Hegu(LI4) area on the peak,area and incubation period changing rate of the zygomatic muscles of the rhesuses showed significant deviation under the physiological condition (p<0.01). The effect of acupuncturing Hegu(LI4) area on the frequency,area and incubation period changing rate of the orbicular muscles of mouth of the rhesuses showed significant deviation under the physiological condition (p<0.05).
The effect of acupuncturing Hegu(LI4) area on the EMG of the the face and mouth were stronger than the EMG acupuncturing Houxi(SI3) and Waiguan(SJ5) area under the physiological condition:The EMG on the frequency,peak,area and incubation period changing rate of the frontal muscles and the orbicular muscles of mouth of the rhesuses acupuncturing Hegu(LI4) area were higher than the EMG acupuncturing Houxi(SI3) and Waiguan(SJ5) area (p<0.05); The EMG on the frequency,peak,area and incubation period changing rate of the zygomatic muscles of the rhesuses acupuncturing Hegu(LI4) area were higher than the EMG acupuncturing Houxi(SI3) and Waiguan(SJ5) area (p<0.01).
②The effect of acupuncturing Hegu(LI4) area on the peak.area and incubation period changing rate of the frontal muscles of the rhesuses showed significant deviation under the general anesthesia condition (p<0.01). The effect of acupuncturing Hegu(LI4) area on the peak.area and incubation period changing rate of the zygomatic muscles of the rhesuses showed significant deviation under the general anesthesia condition (p<0.05). The effect of acupuncturing Hegu(LI4) area on the frequency,peak and area changing rate of the orbicular muscles of mouth of the rhesuses showed significant deviation under the general anesthesia condition(p<0.05).
The effect of acupuncturing Hegu(LI4) area on the EMG of the face and mouth were stronger than the EMG acupuncturing Houxi(SI3) and Waiguan(SJ5) area under the general anesthesia condition:The EMG on the frequency,peak,area and incubation period changing rate of the frontal muscles of the rhesuses acupuncturing Hegu(LI4) area were higher than the EMG acupuncturing Houxi(SI3) and Waiguan(SJ5) area (p<0.01); The EMG on the peak.area and incubation period changing rate of the zygomatic muscles and the orbicular muscles of mouth of the rhesuses acupuncturing Hegu(LI4) area were higher than the EMG acupuncturing Houxi(SI3) and Waiguan(SJ5) area (p<0.01).
③The effect of acupuncturing Hegu(LI4) area on the peak and area changing rate of the frontal muscles of the rhesuses showed significant deviation under the local anesthesia condition (p<0.05). The effect of acupuncturing Hegu(LI4) area on the peak and area changing rate of the zygomatic muscles of the rhesuses showed significant deviation under the local anesthesia condition(p<0.01).
The effect of acupuncturing Hegu(LI4) area on the EMG of the the face and mouth were stronger than the EMG acupuncturing Houxi(SI3) and Waiguan(SJ5) area under the local anesthesia condition:The EMG on the peak,area and incubation period changing rate of the frontal muscles of the rhesuses acupuncturing Hegu(LI4) area were higher than the EMG acupuncturing Houxi(SI3) and Waiguan(SJ5) area (p<0.05); The EMG on the peak and area changing rate of the zygomatic muscles of the rhesuses acupuncturing Hegu(LI4) area were higher than the EMG acupuncturing Houxi(SI3) and Waiguan(SJ5) area (p<0.05).
2. The effect of acupuncturing different acupoints of the healthy people on the facial sensation:The effect of acupuncturing Hegu(LI4) on the facial pain threshold changing rate of the mandibular area of the healthy people showed significant deviation (p<0.05). The effect of acupuncturing Hegu(LI4) comparing with Houxi(SI3) and Waiguan(SJ5) on the facial pain threshold changing rate of the frontal region,the buccal region and the mandibular area of the healthy people showed significant deviation (p<0.05).
3. The effect of acupuncturing different acupoints of the healthy people on the function of salivary glands:The effect of acupuncturing Hegu(LI4) comparing with Houxi(SI3) and Waiguan(SJ5) on the saliva alpha-amylase activity changing rate of the healthy people showed significant deviation (p<0.05).
Conclusions
1. The effect of acupuncturing Hegu(LI4) area on the range and the degree of the impact of the facial muscles of the rhesuses are stronger than Houxi(SI3) area and Waiguan(SJ5) area under the physiological and the general anesthesia conditions. These results suggest that there is relative specificity between Hegu(LI4) area and the facial muscular movement of the rhesuses.
2. The effect of acupuncturing Hegu(LI4) area are weakened on the EMG of the facial muscles of the rhesuses under the local anesthesia condition. These results suggest that the reception of peripheral sensory nerves are important biological basis between Hegu(LI4) area and the face and mouth.
3. The effect of acupuncturing Hegu(LI4) on the degree of the impact of the facial pain threshold and the saliva alpha-amylase activity of the healthy people are stronger than Houxi(SI3) and Waiguan(SJ5). These results suggest that there is relative specificity between Hegu(LI4) and the facial sensation and the salivary glands function of the healthy people.
以“面口合谷收”理论为指导,运用神经肌电检测、痛阂测定、荧光分光光度检测等方法技术,观察针刺合谷穴对恒河猴面口部肌肉运动、正常人感觉传入及唾液腺分泌的影响,证明合谷穴与面口部之间存在特异性联系的基本事实,探索经脉体表特异性联系的生物学基础,进一步丰富经穴效应特异性的科学内涵。
方法
1.针刺不同穴区对恒河猴面口部肌电的影响：按照随机数字表将恒河猴随机分为合谷穴区组、后溪穴区组、外关穴区组,分别在生理、麻醉、局麻三种不同状态下观察记录针剌前后恒河猴面口部额肌、颧肌、口轮匝肌的肌电活动,比较研究针刺合谷穴区、后溪穴区、外关穴区对恒河猴面口部肌电的不同影响。
2.针刺不同穴位对正常人面部感觉及唾液腺分泌功能的影响：运用SPSS软件编程的方法将健康受试者随机分为合谷穴组、后溪穴组、外关穴组,分别针刺健康受试者合谷穴、后溪穴、外关穴,运用钾离子测痛法观察记录针刺前后受试者面部痛阈,运用荧光分光光度法测定针刺前后受试者唾液α-淀粉酶活力,比较研究针刺合谷穴、后溪穴、外关穴对健康受试者面部痛阈及唾液α-淀粉酶活力的不同影响。
结果
1.在生理、麻醉、局麻三种不同状态下针刺不同穴区对恒河猴面口部肌电的影响：①生理状态下针刺合谷穴区对恒河猴额肌肌电的峰峰值、面积及潜伏期有明显影响(P<0.05),对颧肌肌电的峰峰值、面积及潜伏期有明显影响(P<0.01),对口轮匝肌肌电的频率、面积及潜伏期有明显影响(P<0.05)；生理状态下针刺合谷穴区对面口部肌肉肌电的影响明显强于针刺后溪穴区、外关穴区：额肌肌电频率、峰峰值、面积及潜伏期变化率,合谷穴区明显高于后溪穴区、外关穴区(P<0.05)；颧肌肌电频率、峰峰值、面积及潜伏期变化率,合谷穴区明显高于后溪穴区、外关穴区(P<0.01)；口轮匝肌肌电频率、峰峰值、面积及潜伏期变化率,合谷穴区明显高于后溪穴区、外关穴区(P<0.05)。
②麻醉状态下针刺合谷穴区对恒河猴额肌肌电的峰峰值、面积及潜伏期有明显影响(P<0.01),对其颧肌肌电的峰峰值、面积及潜伏期有明显影响(P<0.05),对其口轮匝肌肌电的频率、峰峰值、面积有明显影响(P<0.05)；麻醉状态下针刺合谷穴区对恒河猴面口部肌肉肌电的影响明显强于针刺后溪穴区、外关穴区：额肌肌电频率、峰峰值、面积及潜伏期变化率,合谷穴区明显高于后溪穴区、外关穴区(P<0.01)；颧肌肌电峰峰值、面积及潜伏期变化率,合谷穴区明显高于后溪穴区、外关穴区(P<0.01)；口轮匝肌肌电峰峰值、面积及潜伏期变化率,合谷穴区明显高于后溪穴区、外关穴区(P<0.01)。
③局麻状态下针刺合谷穴区对恒河猴额肌肌电的峰峰值、面积有明显影响(P(0.05),对其颧肌肌电的峰峰值、面积有明显影响(P<0.01)；在局麻状态下针刺合谷穴区对恒河猴面口部肌肉肌电的影响明显强于针刺后溪穴区、外关穴区：额肌肌电峰峰值、面积及潜伏期变化率,合谷穴区明显高于后溪穴区、外关穴区(P<0.05)；颧肌肌电峰峰值、面积变化率,合谷穴区明显高于后溪穴区、外关穴区(P<0.05)。
2.针刺不同穴位对正常人面部感觉的影响：针刺健康受试者合谷穴对其面口部下颌区痛阈有明显影响(P<0.05)；针刺健康受试者合谷穴对其面口部额区、颊区、下颌区痛阈变化率的影响明显强于针刺后溪穴、外关穴(P<0.05)。
3.针刺不同穴位对正常人唾液腺分泌功能的影响：针刺健康受试者合谷穴对唾液α-淀粉酶活力变化率的影响明显强于针刺后溪穴、外关穴(P<0.05)。
结论
1.在生理、全麻状态下针刺合谷穴区对恒河猴面口部的肌电活动有明显影响,其影响范围、影响程度明显大于(强于)针刺后溪穴区、外关穴区的影响,提示恒河猴合谷穴区与面口部肌肉运动之间存在相对特异性的联系。
2.局麻(穴位局部封闭)可明显减弱针刺合谷穴区对恒河猴面口部肌电活动的影响,提示外周感觉神经传入可能是实现合谷穴区与面口部联系的重要生物学基础。
3.针刺合谷穴对正常人面部痛阈、唾液α-淀粉酶活力有明显影响,其影响程度明显强于针刺后溪穴、外关穴的影响,提示正常人合谷穴与面口部感觉、唾液腺分泌功能之间存在相对特异性的联系。
Objectives
To prove the fact of the specific correlation between Hegu(LI4) and face,we used EMG detection, pain threshold detection and fluorospectrophotometry method and observed the effect of acupuncturing Hegu(LI4) on the facial muscular movement of the rhesuses, the facial sensory reception and the salivary glands function of the healthy people by "for the face and mouth,Hegu(LI4) controls"as a guide to explore the biological basis of specific correlation between meridians and the body surface and to rich the scientific content of specificity of meiridians and acupoints effect.
Method
1.The effect of acupuncturing different acupoints area of the rhesuses on the EMG of the facial muscles:We divided the rhesuses into Hegu(LI4) area,Houxi(SI3) area and Waiguan(SJ5) area groups using the random number table.We acupunctured respectively Hegu(LI4) area,Houxi(S13) area and Waiguan(SJ5) area of the rhesuses under the physiological condition and general or local anesthesia conditions to observe and record the changes of the EMG of the frontal muscles.the zygomatic muscles and the orbicular muscles of mouth in order to compare the effect of acupuncturing Hegu(LI4) area,Houxi(SI3) area and Waiguan (SJ5) area of the rhesuses on the EMG of the face and mouth.
2. The effect of acupuncturing different acupoints of the healthy people on the facial sensation and the function of salivary glands:We randomly divided the healthy people into Hegu(L14),Houxi(SI3) and Waiguan(SJ5) groups using SPSS. We acupunctured respectively Hegu(LI4),Houxi(SI3) and Waiguan(SJ5) of the healthy people to observe the changes of the facial pain threshold using the potassium-ion density dolorimeter and detect the changes of the saliva alpha-amylase activity using fluorospectrophotometry in order to compare the effect of acupuncturing Hegu(LI4),Houxi(SI3) and Waiguan(SJ5) of the healthy people on the facial pain threshold and the saliva alpha-amylase activity.
Results
l.The effect of acupuncturing respectively different acupoints area of the rhesuses on the EMG of the facial muscles under the physiological condition and local or general anesthesia conditions:①The effect of acupuncturing Hegu(LI4) area on the peak,area and incubation period changing rate of the frontal muscles of the rhesuses showed significant deviation under the physiological condition (p<0.05). The effect of acupuncturing Hegu(LI4) area on the peak,area and incubation period changing rate of the zygomatic muscles of the rhesuses showed significant deviation under the physiological condition (p<0.01). The effect of acupuncturing Hegu(LI4) area on the frequency,area and incubation period changing rate of the orbicular muscles of mouth of the rhesuses showed significant deviation under the physiological condition (p<0.05).
The effect of acupuncturing Hegu(LI4) area on the EMG of the the face and mouth were stronger than the EMG acupuncturing Houxi(SI3) and Waiguan(SJ5) area under the physiological condition:The EMG on the frequency,peak,area and incubation period changing rate of the frontal muscles and the orbicular muscles of mouth of the rhesuses acupuncturing Hegu(LI4) area were higher than the EMG acupuncturing Houxi(SI3) and Waiguan(SJ5) area (p<0.05); The EMG on the frequency,peak,area and incubation period changing rate of the zygomatic muscles of the rhesuses acupuncturing Hegu(LI4) area were higher than the EMG acupuncturing Houxi(SI3) and Waiguan(SJ5) area (p<0.01).
②The effect of acupuncturing Hegu(LI4) area on the peak.area and incubation period changing rate of the frontal muscles of the rhesuses showed significant deviation under the general anesthesia condition (p<0.01). The effect of acupuncturing Hegu(LI4) area on the peak.area and incubation period changing rate of the zygomatic muscles of the rhesuses showed significant deviation under the general anesthesia condition (p<0.05). The effect of acupuncturing Hegu(LI4) area on the frequency,peak and area changing rate of the orbicular muscles of mouth of the rhesuses showed significant deviation under the general anesthesia condition(p<0.05).
The effect of acupuncturing Hegu(LI4) area on the EMG of the face and mouth were stronger than the EMG acupuncturing Houxi(SI3) and Waiguan(SJ5) area under the general anesthesia condition:The EMG on the frequency,peak,area and incubation period changing rate of the frontal muscles of the rhesuses acupuncturing Hegu(LI4) area were higher than the EMG acupuncturing Houxi(SI3) and Waiguan(SJ5) area (p<0.01); The EMG on the peak.area and incubation period changing rate of the zygomatic muscles and the orbicular muscles of mouth of the rhesuses acupuncturing Hegu(LI4) area were higher than the EMG acupuncturing Houxi(SI3) and Waiguan(SJ5) area (p<0.01).
③The effect of acupuncturing Hegu(LI4) area on the peak and area changing rate of the frontal muscles of the rhesuses showed significant deviation under the local anesthesia condition (p<0.05). The effect of acupuncturing Hegu(LI4) area on the peak and area changing rate of the zygomatic muscles of the rhesuses showed significant deviation under the local anesthesia condition(p<0.01).
The effect of acupuncturing Hegu(LI4) area on the EMG of the the face and mouth were stronger than the EMG acupuncturing Houxi(SI3) and Waiguan(SJ5) area under the local anesthesia condition:The EMG on the peak,area and incubation period changing rate of the frontal muscles of the rhesuses acupuncturing Hegu(LI4) area were higher than the EMG acupuncturing Houxi(SI3) and Waiguan(SJ5) area (p<0.05); The EMG on the peak and area changing rate of the zygomatic muscles of the rhesuses acupuncturing Hegu(LI4) area were higher than the EMG acupuncturing Houxi(SI3) and Waiguan(SJ5) area (p<0.05).
2. The effect of acupuncturing different acupoints of the healthy people on the facial sensation:The effect of acupuncturing Hegu(LI4) on the facial pain threshold changing rate of the mandibular area of the healthy people showed significant deviation (p<0.05). The effect of acupuncturing Hegu(LI4) comparing with Houxi(SI3) and Waiguan(SJ5) on the facial pain threshold changing rate of the frontal region,the buccal region and the mandibular area of the healthy people showed significant deviation (p<0.05).
3. The effect of acupuncturing different acupoints of the healthy people on the function of salivary glands:The effect of acupuncturing Hegu(LI4) comparing with Houxi(SI3) and Waiguan(SJ5) on the saliva alpha-amylase activity changing rate of the healthy people showed significant deviation (p<0.05).
Conclusions
1. The effect of acupuncturing Hegu(LI4) area on the range and the degree of the impact of the facial muscles of the rhesuses are stronger than Houxi(SI3) area and Waiguan(SJ5) area under the physiological and the general anesthesia conditions. These results suggest that there is relative specificity between Hegu(LI4) area and the facial muscular movement of the rhesuses.
2. The effect of acupuncturing Hegu(LI4) area are weakened on the EMG of the facial muscles of the rhesuses under the local anesthesia condition. These results suggest that the reception of peripheral sensory nerves are important biological basis between Hegu(LI4) area and the face and mouth.
3. The effect of acupuncturing Hegu(LI4) on the degree of the impact of the facial pain threshold and the saliva alpha-amylase activity of the healthy people are stronger than Houxi(SI3) and Waiguan(SJ5). These results suggest that there is relative specificity between Hegu(LI4) and the facial sensation and the salivary glands function of the healthy people.
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