摘要
拟穴青蟹(Scylla paramamosain,简称青蟹)是我国东南沿海地区重要的海洋经济养殖蟹类,之前我国有关锯缘青蟹的研究报道实际上都应归为拟穴青蟹。拟穴青蟹属于广温、广盐、肉食性名贵海产品,营养丰富,肉质好,深受人们喜爱。青蟹是汕头市传统特色优势海水养殖品种,但是,自2004年起,汕头市青蟹养殖遇到了严重的病害问题,每年9~11月,牛田洋围垦区养殖的青蟹病害严重,给当地经济造成了很大损失。近几年来,本课题组对牛田洋养殖青蟹的病害问题进行了较系统深入的研究,已弄清病害的主要类型、病原和病因,查明养殖水体理化因子的恶化和条件致病菌的大量增生是牛田洋青蟹病害爆发的重要原因之一;研究利用中草药进行防治病害的方法,取得一定成效。
在上述研究工作的基础上,本论文从生态角度出发,期望通过建立青蟹健康养殖模式,达到防控和降低病害发生的目的。养殖试验在3个半咸水养殖池塘中进行(1号和2号池塘面积各1亩,3号池塘15亩),各池塘放养种类和密度相同(拟穴青蟹与斑节对虾、鲻鱼混养)但养殖模式不同。1号和2号池塘采用半封闭式养殖模式,而3号池塘采用开放式养殖模式,养殖周期共46周;其中,在养殖试验进行35周后,在1号池塘按21Kg/亩放入细基江篱,共养殖约10周。通过比较不同池塘水体理化因子及微生物的变化及差异,探讨半封闭式养殖模式与传统开放式养殖模式的不同点,以及细基江篱对水体环境的修复作用。主要结果如下:
与传统开放式养殖模式相比,半封闭式养殖模式养殖水体盐度相对稳定;溶解氧含量较高,有害物质氨氮含量低且稳定在0.01mg/L~0.11mg/L范围内, 7月、10~12月显著低于开放式养殖模式(P<0.05);6~11月水体的无机磷水平,5~7月和9~11月水体的异养菌含量,以及5月、8月、10~12月水体的弧菌含量都显著低于开放式养殖模式(P<0.05)。半封闭模式在未放养细基江蓠情况下,其拟穴青蟹的存活率及池塘亩净收益分别比传统养殖模式提高66.22%和38.46%。
放养细基江篱的半封闭式池塘与未放养细基江蓠的半封闭对照池塘相比,水体中溶解氧含量在放养细基江篱10天开始显著高于对照池塘(P<0.05);盐度在放养细基江篱20天开始显著低于对照池塘(P<0.05),并且趋于稳定;放养细基江篱后,水体中氨氮含量从江篱投放20天时的0.29mg/L显著下降到40天时的0.04mg/L(P<0.05)。总体来说,水体中大多数重要理化指标都朝着有利于养殖品种健康生长的方向变化,收获时拟穴青蟹的存活率及池塘亩净收益分别比对照池塘提高22.43%和、60.47%,比传统养殖模式提高103.51%和、122.20%。
本研究结果表明,半封闭式养殖模式与传统开放式养殖模式相比,受外界水源质量影响较小,水体中各理化指标及微生物含量没有开放式养殖模式变化剧烈,给各养殖品种提供了一个稳定的生存环境,因此在各品种产量、存活率及收益等方面均高于传统开放式养殖模式;而在半封闭式养殖模式中,加入细基江篱对其水质理化指标产生了明显的改善作用,更加有利于各养殖品种的健康生长,提高了产量、存活率及收益。因此,将拟穴青蟹与斑节对虾、鲻鱼混养,并在养殖后期加入细基江篱的半封闭式养殖模式可以作为一种拟穴青蟹的健康养殖模式加以推广。
The mud crab Scylla paramamosain is the dominant species of Scylla cultured along the coast of southern China and also a traditionally cultured species in Shantou. The previous researches on S. serrata (Forskal) in China actually belong to those of S. paramamosain. It is a predacious, eurythermal and euryhaline crustacean with rich nutrition and high quality of meat. However, since 2004, the culture of crab in the Niutianyang culture zone of Shantou has being faced with serious diseases from September to November, and this resulted in high mortality and great economic loss. In recent years, we have done systematic studies on the diseases including the clarities of majoy types, pathogenies and cause of diseases. It was demonstrated that the deterioration of environmental factors and the proliferation of conditional pathogens are the important reasons for the outbreak of crab diseases, and the Chinese medicinal herb showed a positive effect on the controlling and cure of crab diseases.
On the basis of above studies, this thesis conducted studies from the ecological aspects, with a purpose to establish a healthy culture pattern for mud crab so as to reduce risks of diseases outbresk. Three brakish water ponds (number 1 and 2 was 667 m~2, and number 3 was 15×667 m~2) were chosen to do the experiments, each pond had a same stocking density of mud crab, sugpo prawn Penaeus monodon and mullet Mugil cephalus (Linnaeus). However, the culture mode was different. The number 1 and 2 ponds were semi-enclosed breeding mode, and number 3 pond was the traditional open breeding mode. Total period of culture was 46 weeks. After 35 weeks, the Gracilaria tenuistipitata was put into pond 1 until the end of experiment. By comparing the variation and diversity of water physical and chemical parameters, and microorgamism as well, differences between the semi-enclosed breeding and open breeding modes, and the repairation of Gracilaria tenuistipitata on culture water were investigated. The results as follows:
Compared with the traditional open breeding mode, a more stable water salinity, higher content of dissolved oxygen and lower ammonia nitrogen were observed in the semi-enclosed breeding mode, in which the content of ammonia nitrogen was low and stable in 0.01mg/L~0.11mg/L, which was significantly lower than that in the open breeding mode in July and from October to December (P<0.05). Besides, the levels of inorganic phosphorus from June to November, heterotrophic bacteria from May to July and September to November, and vibrio bacteria in May, August and from October to December in the water of semi-enclosed breeding were significantly lower than those on open breeding mode (P<0.05). Compared with the traditional open breeding mode, the mud crab’s enhance rate of survival rate and net earning per 667 m~2 were respectively 66.22% and 38.46% higher in the semi-enclosed breeding mode’s without G. tenuistipitata.
Comparied with the semi-enclosed breeding mode without G. tenuistipitata, the addition of G. tenuistipitata into pond of semi-enclosed breeding mode increased the level of dissolved oxygen which was significantly higher (P<0.05) after 10 days, the salinity was significantly lower (P<0.05) after 20 days and became stable. Water level of ammonia nitrogen was from the initial 0.29mg/L down to 0.04mg/L after 20 days. In general, most of the water parameters became beneficial to animal growth. And the mud crab’s enhance rate of survival rate and net earning per 667 m~2 in the semi-enclosed breeding mode with G. tenuistipitata was respectively 22.43% and 60.47% higher than those in the same mode without G. tenuistipitata, and 22.43% and 60.47% higher than traditional open breeding mode respectively.
The present study showed that, compared with the raditional open breeding mode, the water environment of pond with semi-enclosed breeding mode was less influenced by the outside water source, and the physical and chemical characters did not changed so fiercely. Therefore such a mode can give the cultured animals a stable living environment, and thus the yield, survival rate and profit are higher than those in the open breeding mode. The addition of G. tenuistipitata into pond of semi-enclosed breeding mode can obviously improve the water characters, which is good for the healthy growth of cultured animals, and increases the yield, survival rate and profit. So, the semi-enclosed breeding mode mainly cultured crab with P. monodon and M. cephalus, and added with G. tenuistipitata in later stage can be used as a healthy culture mode for crab and worth of generalization.
引文
蔡继晗,沈奇宇,郑向勇,等.氨氮污染对水产养殖的危害及处理技术研究进[J].浙江海洋学院学报(自然科学版),2010(02):167-172,195.
曹凯德.对虾养殖过程中的水质控制[J].中国水产,2002(4):57-58.
曾呈奎,夏邦美.中国海藻志[M]北京:科学出版社,1999:第二卷,71-73.
陈华,刘如玉.锯缘青蟹副溶血性弧菌的分离与鉴定[J].福建中医学院学报,2009,19(2): 26-27, 70.
陈家长,简纪常,胡庚东,等.利用有益微生物改善养殖生态环境的研究[J].湛江海洋大学学报,2002,22(4):33-36.
陈建明,沈斌乾,潘茜,等.拟穴青蟹大眼幼体和幼蟹对盐度的适应性研究[J].中国水产. 2010(6): 68-69.
陈培兴.罗氏沼虾与淡水青虾的轮作养殖[J].渔业现代化. 2000(1): 5-6.
陈琴,陈晓汉,罗永巨,等.南美白对虾耗氧率和窒息点的初步测定[J].水利渔业,2001(02).
陈宇锋,艾春香,林琼武,等.盐度胁迫对锯缘青蟹血清及组织、器官中PO和SOD活性的影响[J].台湾海峡,2007,26(4):569-575.
陈祖洪.水化学[M]北京:中国农业出版社,2004:,203-207.
丁理法,陈飞,李永富,等.锯缘青蟹与脊尾白虾生态养殖技术研究[J].中国水产,2009(9).
董志国.水产动物的健康养殖[J].中国饲料,2003(11).
杜瑜,史为良.水的温度分层与水产养殖的关系[J].中国水产,2006(03),81-82.
冯兴钱,方家仲.青蟹养殖技术[M]杭州:浙江科学技术出版社,1996:,206-215.
冯振飞,王国良,钱冬,等.锯缘青蟹养殖环境中细菌类群及其数量分布[J].水产科学,2008, 27(11):574-577.
广东省水产局对虾养殖办公室.斑节对虾养殖实用技术[M].广州:广东科技出版社,1992.
郭秀云,王胜,吴必文,等.环境温度对水产养殖定量化影响的研究[J].安徽农业科学, 2007(24),7498-7499.
纪灵,任荣珠,梁源高,等.烟台近岸海域无机磷含量逐年上升的原因分析[J].海洋通报, 1998(06) ,21-27.
居礼,王玉堂,蒋宏斌,等.海水鱼类集约化养殖技术[M]北京:海洋出版社,2004:,273-280.
金乃康,朱迪.罗氏沼虾网箱养殖技术[J].北京水产. 2000: 19.
孔祥勇,朱本才,郑建平,等.藕池养殖罗氏沼虾试验[J].齐鲁渔业. 2000, 17(4): 11-12.
李海兵,宋晓玲,李赟,等.水产动物益生菌研究进展[J].动物医学进展,2008(05).
罗俊标,李望东,盘润洪,等.罗非鱼养殖技术之二吉富罗非鱼和罗氏沼虾混养试验和推广[J].中国水产. 2004(9): 48-49.
陆立刚.罗氏沼虾大棚加温养殖技术[J].中国水产. 1999(1): 23.
林琪,李少菁,黎中宝,等.中国东南沿海青蟹属不同种类的mtDNA COI基因序列分析及其系统发育[J].厦门大学学报(自然科学版). 2008, 47(2): 268-273.
李荣俭.罗氏沼虾大棚越冬技术[J].科学养鱼. 2009(1): 38.
路心平.青蟹属的系统进化及中国沿海拟穴青蟹的群体遗传结构研究[D].中国科学院海洋研究所, 2008.
李云峰.锯缘青蟹养殖池塘底充式增氧技术试验[J].农家之友·现代农业研究,2009(14): 73-93.
梁志辉,王志成,杨家林,等.锯缘青蟹大眼幼体与凡纳滨对虾混养试验[J].水产科学,2007, 26(11):606-609.
林琼武,李少菁,王桂忠,等.锯缘青蟹和斑节对虾北移养殖试验[J].福建水产,2005(107): 39-42.
马月钗,杨玉娇,王国良.盐度变化对锯缘青蟹Scylla serrata免疫因子的胁迫影响[J].浙江农业学报,2010(04).
穆占昆,杨振国,周玉,等.中国对虾和三疣梭子蟹混养试验[J].水产科学,2001,20(5):16-18.
潘勇,李如桐.稻田养殖罗氏沼虾高产报告[J].北京水产. 2000(6): 25.
邵锦淑.虾池双茬菲律宾蛤仔和对虾、褐篮子鱼混养实验研究[J].福建水产,2007(4):17-19.
申玉春,叶富良,梁国潘,等.虾—鱼—贝—藻多池循环水生态养殖模式的研究[J].湛江海洋大学学报,2004,24(4):10-16.
宋盛宪,古群红,庞德彬.锯缘青蟹无公害养殖技术[M].北京:海洋出版社,2006.
檀东飞,吴国欣,林跃鑫,等.锯缘青蟹营养成分分析[J].福建师范大学学报(自然科学版), 2000,16(4):79-84.
王大鹏,田相利,董双林,等.对虾、青蛤和江蓠三元混养效益的实验研究[J].中国海洋大学学报,2006,36:20-26.
王国良.塑料大棚养殖二茬罗氏沼虾技术[J].中国水产. 2003(12): 43-44.
王夏龙,王潮林.锯缘青蟹塘套养鲻(梭)鱼技术初探[J].内陆水产,2007(4):24-25.
王彦波,许梓荣,邓岳松.水产养殖中氨氮和亚硝酸盐氮的危害及治理[J].饲料工业,2002 (12).
魏泰莉,余瑞兰,聂湘平,等.养殖水环境中亚硝酸盐对鱼类的危害及防治的研究[J].水产养殖,1999(03):15-17.
魏万权,林仕梅.水产养殖中溶解氧的研究[J].饲料工业,2007(16),20-23.
吴斌,廖思明,兰国宝.水生无脊椎动物氮排泄研究概述[J].广西科学,2008,15(1):92-96.
吴中华,刘昌彬,刘存仁,等.中国对虾慢性亚硝酸盐和氨中毒的组织病理学研究[J].华中师范大学学报(自然科学版),1999(01) ,119-122.
吴清洋.汕头牛田洋锯缘青蟹病害发生的环境因素及中草药防治研究[D].广东汕头:汕头大学, 2009.
吴清洋,李远友,夏小安,等.汕头牛田洋沿海围垦区锯缘青蟹病害爆发的环境因素[J].生态学报,2010,30(8),2043-2048.
相建海.海水养殖生物病害发生与控制[M].北京:海洋出版社,2001.
徐华,艾春香,林琼武,等.盐度胁迫对锯缘青蟹体内腺苷三磷酸酶和磷酸酶活性的影响[J]. 农业环境科学学报,2007(03).
夏小安.汕头牛田洋锯缘青蟹病害研究[D].广东汕头:汕头大学, 2008.
杨峰,张量.罗氏沼虾大棚暂养阶段罗氏沼虾大棚暂养阶段池塘亚硝酸盐偏高的原因分析及处理方法[J].科学养鱼. 2010(3): 27.
袁盛贤.罗氏沼虾大棚苗阶段亚硝酸盐偏高原因及对策[J].科学养鱼. 2007(2): 81.
于忠利,乔振国,王建钢.底质和遮蔽物在锯缘青蟹Scylla serrata(Forslcal)种苗中间培育中的作用[J].现代渔业信息,2008,23(5):26-28.
袁春营,崔青曼.盐度影响水产养殖动物生长发育的研究进展[J].水产科学,2004(05),41-42.
张福芸,韩世华,尹学福,等.北方地区低盐度池塘锯缘青蟹养殖技术[J].中国水产,2005 (10):40-41.
张宏刚,程忠,史国经,等.江黄颡、鳜鱼、青虾混养试验[J].北京水产,2004:18-20.
郑春波,王世党,于诗群,等.三疣梭子蟹、南美白对虾、菲律宾蛤子高效综合养殖[J].水产养殖,2009(3):21-23.
周维武,姜启平,郑春波,等.锯缘青蟹浅海吊笼养殖的可行性分析[J].中国水产,2005(10):43-44.
周鑫,王素英,池凌钰,等.海水养殖废水氨氮降解菌的筛选及培养条件研究[J].安徽农业科学,2009,37(11):4908-4910.
Alejandro H. Buschmann, Daniel A. Varela, Mmaría Carmen Hernández-González, et al. Opportunities and challenges for the development of an integrated seaweed-based aquaculture activity in Chile: determining the physiological capabilities of Macrocystis and Gracilaria as biofilters[J]. J Appl Phycol,2007,20:571-577.
Benjamas Chuntapa, Sorawit Powtongsook, Piamsak Menasveta. Water quality control using Spirulina platensis in shrimp culture tanks[J]. Aquaculture,2003,220:355-366.
Brito R., Chimal M. E., Rosas C.. Effect of salinity in survival, growth and osmotic capacity of early juveniles of Farfantepenaeus brasiliensis (Decapoda: Penaeidae)[J]. Journal of Experimental Marine Biology and Ecology,2000,244:253-263.
Castinho P. C., Martins I. A.,'Bianchini A.. Gill Na~+, K~+-ATPase and osmoregulation in the estuarine crab, Chasmagnathus granulate Dana, 1851(Decapoda, Grapsidae)[J]. Journal of Experimental Marine Biology and Ecology,2001,256:215-227.
Cheng W., Liu C. H., Hsu J. P.. Effect of hypoxia on the immune response of the giant freshwater prawn Macrobrachium rosenbergii and its susceptibility to pathogen Enterrococcus[J]. Fish & Shellfish immunology,2002,13:351-365.
Cheng W., Chen J. C.. Effects of pH, temperature and salinity on immune parameters of the freshwater prawn Macrobrachium rosenbergii[J]. Fish & Shellfish immunology, 2000, 10: 387-391.
DerunYuan, Yang Yi, Amararatne Yakupitiyage, et al. Effects of addition of red tilapia (Oreochromis spp.) at different densities and sizes on production, water quality and nutrient recovery of intensive culture of white shrimp (Litopenaeus vannamei) in cement tanks[J]. Aquaculture,2010,298:226-238.
Donald M. Anderson. Red Tide[J]. Scientific Amercian,1994,271(2):52-58. Donny Ponce-Marbán, Juan M. Hernández, Eucario Gasca-Leyva. Simulating the economic viability of Nile tilapia and Australian redclaw crayfish polyculture in Yucatan, Mexico[J]. Aquaculture,2006,261:151-159.
Eleonor A. Tendencia, Milagros R. dela Pena,Casiano H. Choresca Jr. Effect of shrimp biomassand feeding on the anti-Vibrio harveyi activity of Tilapia sp. in a simulated shrimp–tilapia polyculture system[J]. Aquaculture,2006,253:154-162.
H. Shan, J. P. Obbard. Ammonia removal from prawn aquaculture water using immobilized nitrifying bacteria[J]. Appl Microbiol Biotechnol,2001,57:791-798.
Jones A. B., Preston N. P., Dennison W. C. The efficiency and condition of oysters and macroalgae used as biological filters of shrimp pond effluent[J]. Aquaculture Research,2002,33(1):1-19.
K. Haglund, M. Pedersén. Growth of the Red Alga Gracilaria tenuistipitata at High pH. Influence of Some Environmental Factors and Correlation to an Increased Carbonic-anhydrase Activity[J]. Botanica Marina,1992,35(6):579-588.
Le Moullac G., Haffner P.. Environmental factors affecting immune responses in Crustacean[J]. Aquaculture,2000,191:121-131.
Lei Yang, Lin-Sen Chou, Wen K. Shieh. Biofilter Treatment Of Aquaculture Water For Reuse Applications[J]. Wat. Res.,2001,35(13):3097-3108.
Li Y. Y., Xia X. A., Wu Q. Y., et al. Infection with Hematodinium sp. in mud crabs Scylla serrata cultured in low salinity water in southern China. Diseases of Aquatic Organisms, 2008, 82:145-150.
Luis R. Martinez-Cordova, Marcel Martinez-Porchas. Polyculture of Pacific white shrimp, Litopenaeus vannamei, giant oyster, Crassostrea gigas and black clam, Chione fluctifraga in ponds in Sonora, Mexico[J]. Aquaculture,2006,258:321-326.
Mst. Muslima Khatun, Dipak Kamal, Kou Ikejima, et al. Comparisons of growth and economic performance amongmonosex andmixed-sex culture of redmud crab (Scylla olivacea Herbst, 1796) in bamboo pens in the tidal flats of mangrove forests, Bangladesh[J]. Aquaculture Research,2009,40:473-485.
Maloy Kumar Sahu, N. S. Swarnakumar. Probiotics in aquaculture: importance and future perspectives[J]. Indian J. Microbiol.,2008.
Mikulski C. M., Burnett L. E., Burnett K. G.. The effect of hypercapnic hypoxia on the survival of shrimp challenged with Vibrio parahaemolyticus.[J]. The Journal of Shellfish Research,2000,19:301-311.
Pedro Daleo, Tomás Luppi, Agustina Mendez Casariego, et al. The efect of size and cheliped autotomy on sexual competition between males of the mud crab Cyrtograpsus angulatusDana[J]. Mar Biol,2009,156:269-275.
Pikul Jiravanichpaisal, Stefan Roos, Lennart Edsman, et al. A highly virulent pathogen, Aeromonas hydrophila, from the freshwater crayfish Pacifastacus leniusculus[J]. Journal of Invertebrate Pathology,2009,101:56-66.
Randall D. J., Tsui T. K. N.. Ammonia toxicity in fish[J]. Marine Pollution Bulletin, 2002, 45: 17-23.
T. L. Slaneya, K. D. Hyattb, T. G. Northcotec, et al. Status of Anadromous Salmon and Trout in British Columbia and Yukon [J]. Fisheries ,1996,21(10):20-35.
Xiangli Tian, Deshang Li, Shuanglin Dong, et al. An experimental study on closed-polyculture of penaeid shrimp with tilapia and constricted tagelus[J]. Aquaculture,2001,202:57-71.
Ying-Feng Lin, Shuh-Ren Jing, Der-Yuan Lee, et al. Nutrient removal from aquaculture wastewater using a constructed wetlands system[J]. Aquaculture,2002,209:169-184.