胺基化磁性壳聚糖微球固定Ct处理含酚废水研究
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摘要
本文报道三方面的内容：(1)对耐酚热带假丝酵母Candida tropicalis(简写为Ct)的优化发酵条件进行研究，并用正交实验法确定了其除酚发酵的最适培养基。(2)制备用于固定Ct的载体，用反相悬浮聚合法合成磁性壳聚糖微球，再进行化学改性，引入胺基，使其碱性增强，成为新型的磁性壳聚糖胺基衍生物，并用元素分析法对其进行了表征。然后，分别以磁性壳聚糖微球和胺基化磁性壳聚糖微球为载体固定Ct。结果表明：以改性后的胺基化磁性壳聚糖微球为载体固定Ct的理化性质优于磁性壳聚糖微球。(3)初步探讨了胺基化磁性壳聚糖微球固定Ct对模拟含酚废水的催化氧化效率和对实际废水的处理情况。结果表明：在模拟含酚废水处理中，胺基化磁性壳聚糖微球固定Ct的反应活性比磁性壳聚糖微球固定Ct高；同时将其用于福建泉港炼油厂的含酚废水处理，也取得了很好的效果。
This paper reports three aspects as follow: First, the optimum
composition of a fermentative medium for phenol-resisting Candida tropicalis was decided by the orthogonal test. Second, a new carrier to immobilized Candida tropicalis was synthesized. A novel aminated magnetic chitosan microspheres were prepared from chitosan flake through inverse suspension cross-linking in the presence of magnetic particles, then hydroxylpropyl chloridization and amination. Besides, it was characterized by Element Ultimate Analysis. Then, as a carrier, the immobilization of Candida tropicalis on aminated magnetic chitosan microspheres and magnetic chitosan microspheres were studied. The results showed that aminated magnetic chitosan microspheres immobilizing Candida tropicalis had the better physicochemical properties than magnetic chitosan microspheres immobilizing Candida tropicalis. Third, probed into the catalytic oxidizing efficiency of aminated magnetic chitosan micropheres immobilizing Candida tropicalis used to deal with man-made and factory wastewater. With regard to man-made wa
stewater, the results showed that the reaction activity of aminated magnetic chitosan microspheres immobilizing Candida tropicalis was superior to magnetic chitosan micropheres immobilizing Candida tropicalis. At the same time , we also gained a good effect as to factory wastewater which from oil refinery in QuanGang area of Fujian province.
This paper reports three aspects as follow: First, the optimum
composition of a fermentative medium for phenol-resisting Candida tropicalis was decided by the orthogonal test. Second, a new carrier to immobilized Candida tropicalis was synthesized. A novel aminated magnetic chitosan microspheres were prepared from chitosan flake through inverse suspension cross-linking in the presence of magnetic particles, then hydroxylpropyl chloridization and amination. Besides, it was characterized by Element Ultimate Analysis. Then, as a carrier, the immobilization of Candida tropicalis on aminated magnetic chitosan microspheres and magnetic chitosan microspheres were studied. The results showed that aminated magnetic chitosan microspheres immobilizing Candida tropicalis had the better physicochemical properties than magnetic chitosan microspheres immobilizing Candida tropicalis. Third, probed into the catalytic oxidizing efficiency of aminated magnetic chitosan micropheres immobilizing Candida tropicalis used to deal with man-made and factory wastewater. With regard to man-made wa
stewater, the results showed that the reaction activity of aminated magnetic chitosan microspheres immobilizing Candida tropicalis was superior to magnetic chitosan micropheres immobilizing Candida tropicalis. At the same time , we also gained a good effect as to factory wastewater which from oil refinery in QuanGang area of Fujian province.
引文
[1] 邱保国．环境保护．1997，(10)：15．
[2] 杨智宽，韦进宝．<<污染控制化学>[M]．湖北：武汉大学出版社．1998：402．
[3] 张锦，李圭白，马军．化学工程师．2001，(2)：36．
[4] 黄志勇，陈国树．环境与开发．1997，12(2)：32．
[5] 林齐平．水处理技术．1993，19(2)：174．
[6] 乌锡康．<<有机废水处理>．[M]北京：化学工业出版社．1999：147．
[7] 张芳丙，金承基等．<<含酚废水的处理与利用>[M]．北京：化学工业出版社．1983．
[8] 张全兴，刘天华．化工环保．1994，14：344．
[9] Magne P.and Walker EL. Carbon. 1986,24(2):101.
[10] 戴蝤元，杨义燕，杨天雪．化工进展．1991，6：40．
[11] 孙贤波，杜慧芳．环境化学．1999，18(1)：46．
[12] 潘碌亭，肖锦．膜科学与技术．1999，19(5)：19．
[13] 曾炳蔚，郑景涛．化工时刊．1997，11(7)：18．
[14] Yinhua Wan,Xiangde Wang,Xiujuan Zhang. J Membrane Science. 1997,135 (2): 263.
[15] Jiri S Sb Praci Vyzkumn Chem,Vynziti Uhli, Dehtu Ropy.No.1992,2: 397.
[16] 唐守映等．环境科学研究．1995，8(6)：37．
[17] 王检．环境工程．1994，12(6)：16．
[18] Zumbrunn J P, Crommelynck F.Ger, Offen. 1990,222853.
[19] 刘勇弟等．中国环境科学．1994，14(5)：341．
[20] Rajko K Tehnika. 1959,14,008f～200k..
[21] Mikhailova M et al. sb Dokl, -Natskonf. Mladite Nauchni Rab. Spets."Neft Khim",1st,1976;327.
[22] Arai shigeru et al.Japan.Kokai. 1975,74 44554.
[23] Kharlamova T A,gorkhova LT.Khim. Yekhnol Vody. 1982,4(2):157.
[24] Fiti M .Report, 1975 AED-CONF 75-061-015.
[25] 杨琦，单立志，钱易．环境科学研究．1998，11 (4)：62．
[26] Hu,X.,Lei.1.,Chen,G and Yue,P.L. Water Res .2001,35(8):2078.
[27] Li L,et al. J AICHE,1991,37(11):1678.
[28] Barner H.E.,Huang C.Y., Johnson T.et al. J of Hazardous Materials. 1992,31:1.
[29] 赵金辉，张克荣等．中国卫生检验杂志．2000，10 (1)：5．
[30] Mario Schiavello, Kluwer Academic Publishers. 1998.
[31] R.Bauer ,G.Waldner. Catalysis Today. 1999,53:131.
[32] Lei,L.,Hu,X.et al. J of Photochemistry and Photobiology A-Chemistry.1998,16:159.
[33] 胡春，王怡中．环境科学．1997，18(4)：1．
[34] 魏宏斌．中国给水排水．1999，15(2)：14．
[35] 陈英，钟理．化工进展．1999，18(5)：26．
[36] 王建龙，施汉昌，钱易．环境科学研究．1999，12(1)：60．
[37] Si-Jing Wang,Kai-Chee Loh. Biotechnol.Bioeng.2000,68(4):437.
[38] Kira.A.Onysko,HectorM.Budman,CampbellW.Robinson.Biotechnol.Bioeng.2000,70(3):291.
[39] Garcia I.,Bonilla Venceslada J.L.,et al. Water Res. 1997,31(5):2005.
[40] Conner M.A.and Allgeier R.J.Adv. Appl. Microbiol. 1976,28:878.
[41] Chibata Z. and Tosa T. Appl. Biochem. Bioeng. 1983,4:1.
[42] Scott C.D. Enzyme Microb. Technol. 1987,9;66
[43] 王建龙著．《生物固定化技术与水污染控制》[M]．北京：科学出版社．2002．
[44] M Fujimura et al. Appl Microbiol Biotechnol. 1984,19(1):79.
[45] I S Maddox et al. Biotechnol. Bioeng. 1981,23(1):349.
[46] O S Inloes et al. Biotechnol. Bioeng. 1983,25(5):2653.
[47] Grubhofer N. And Schleith L. Naturwissenschafier. 1953,40:508.
[48] Campbell D. H. Luescher E. and Lerman, L.S Proc. Natl.Acad.Sci,USA, 1951,37:575
[49] Tosa T, Mori T, Fuse N. And Chibata I, Enzymolgia. 1966,31;214.
[50] Nunez M.J and Lema J.M. Enzyme Microb. Technol. 1987,9:642.
[51] Bimbaum S, Larsson, P. O. Nisbach, K. 1986, Pergamon Press, Elmsford, New York:45.
[52] 千佃一郎等．《固定化酵素》[M]．论坛社，东京．1975．
[53] 居乃琥，全国固定化生物催化剂学术讨论会报告．1988．
[54] Arcuri E.J et al .Biotechnol. Bioeng. 1982,14:593.
[55] 居乃琥．食品与发酵工业．1981，No．6：30．
[56] Baker D A,et al, J. Inst. Brew.1978,79:487.
[57] Kolot F. B. Process Biochemistry. 1980,15(7):2.
[58] Kinner N.E. and Eighmy, T.T,J.Water Pollut, Control Fed. 1985,57:526.
[59] Bidey s.p, In immobilized cells and enzymes, a practical Approach (Woodward, J,ed),CRL Press, Oxford. 1985,147.
[60] 居乃琥等编著．《固定化酶理论与应用．》[M]．北京：轻工业出版社．1987：169．
[61] Cabral,J.M.S,et al.Ann. N. Y.Acad. Sci. 1984,434:483.
[62] Van Haecht J.L.et al. Biotechnol, Bioeng. 1985,27:217.
[63] Krug T.A,and Dauglis A.J,Biotechnol. Lett. 1983,5:159.
[64] Gainer J.L.and Kirwan D.J. Ann, N,Y, Acad, Sci. 1984,434:465.
[65] Daugulis A.J,et al, Biotech. Bioeng. 1985,27:626.
[66] Okita W.B.,et al., Biotechnol. Bioeng. 1985,27:634.
[67] Chibata I. Et al, Appl. Microbiol. 1974,27:878.
[68] Snell R.L.,U.S.Patent. 1976,No.3:974.
[69] Mohan R.R. and Li N.N., Biotechnol. Bioeng. 1975,17:1137.
[70] Lim F. and Sun A.M., Science. 1980,210:908.
[71] 居乃琥．工业微生物．1989，199(1)：25．
[72] Cavin J .F.,et al.,Biotechnol. Lett, 1985,7:821.
[73] Linko P.,et al.,Ann N.Y.Acad. Sci. 1984,434:406.
[74] Loung J.H.,et al.,Appl. Microbiol. Biotechnol. 1984,19:207.
[75] Chibata I.,et al. In Manual of Industrial Microbiology and Biotechnology American Society for Microbilogy, Washington, D.C. 1986,217.
[76] Takamatsu S.,et al., Eur. J. Appl. Microbiol. Biotechnol. 1982,15:147.
[77] Bang W.G., et al., Biotechnol. Bioeng. 1983,25:1013.
[78] Fujimura M., et al., Appl. Microbiol. Biotechnol. 1984,19:136.
[79] O shea G.M.,et al., Biochim. Biophys Acta. 1984,804:133.
[80] Goosen M. F.A., et al., Biotechnol. Bioeng. 1985,27:146.
[81] 居乃琥．生物工程研究进展．1987，No5，1．
[82] Mcloughlin A.J., Int. Indust .Biotechnol. 1986,6:180.
[83] Congress of the United states. OTA Report on Biotechnology, Commercial Biotechnology. 1984.
[84] 田中渥夫，福井三郎．化学工学．1978，43：269．
[85] Tanaka.A.,et al., Eur. J.Appl. Microbiol. Biotechnol. 1978,5:17.
[86] H Bettmann et al. Appl. Microbiol. Biotechnol. 1984,20:285.
[87] H Bettmann et al. Appl.Microbiol. Biotechnol. 1985,22:289.
[88] F. Westmeier et al.Appl.Microbiol. Biotechnol. 1985,22:301.
[89] 周相林等．环境科学学报．1986，6(3)：368．
[90] 陈陶声．《固定化酶理论与应用》[M]．北京：轻工业出版社．1987．
[91] 黄武华等．环境科学学报．1982，2(4)：293．
[92] A M Anselmo et al．应用微生物．1987，(3)：59．
[93] H M Ehrhardt et al. Appl Microbiol Biotechnol.1989,30(3):312.
[94] 孙艳，李京，谭立扬．环境科学研究．1999，12(1)：1．
[95] Sofer, S.S.J Water Pollut. Control Feed. 1990,62(1):73.
[96] K T O Reilly. et al. Appl. Environ. Microbiol. 1996,55(9):2113.
[97] K T O Reilly et al. In:IAWPRC conference on water and wastewater microbiology. Newport Beach, CA,U.S.A. 1996,8.
[98] 蒋文锐等．环境科学．1981，2(3)：196．
[99] 杨连栋．水处理技术．1990，16(3)：223．
[100] J P Kutney et al. Appl. Environ. Microbiol. 1988,54(4):1015.
[101] J Beuuink et al. Appl Microbiol Biotechnol. 1988,29(1):72.
[102] B Bisping et al. Biotechnol. Appl. Biochem. 1988,10 (2):87.
[103] H J Gijen et al. Biotechnol. Lett. 1988,10(1):61.
[104] L E Macaskie et al. Biotechnol. Bioeng. 1987,30(1):66.
[105] Geoffrey W Garnham, Geoffrey A Codd, Geoffrey M Gadd. Environ. Sci. Technol. 1992,26:1764.
[106] 胡庆晏等．环境污染与防治．1993，25(1)：35．
[107] 黄晓维，王春生，王孔星等工业水处理．1989，9(5)：23．
[108] 梁沈平，王菊思．环境科学．1998，19(4)：13．
[109] 王孔星，张秀文，谢裕敏等．环境科学与技术．1990，2(2)：2．
[110] 刘志培，杨惠芳，贾省芳等．环境科学．1992，13(1)：2．
[111] Livernoche, D, et al., Biotechnol. Bioeng. 1983,25(9):2055.
[112] S. Ohmomo, et al., Agric. Biol. Chem. 1988,52(10):2437.
[113] 蒋挺大．《壳聚糖》[M]．北京：化学工业出版社．2001．
[114] Stegemann H.Z.Physiol. Chem. 1963,331:269.
[115] Herzog K H,Grossmann H,Liefiaender M.Z.Physiol .Chem. 1975,356:1067.
[116] Surarit R, Gopel P K,Shepherd M G. J.Gem,Microbiol. 1988,134:1723.
[117] 倪红，杨艳红，陈怀新．湖北大学学报 (自科版)．2001(1)：77．
[118] Chuang W Y, Young T H,Yao C H,et al. Biomaterials. 1999,20:1479.
[119] Koyano T, Koshizaki N,Umehara H, et al. Polymer. 2000,41:4461.
[120] Mucha M,Piekielna J, Wieczorek A. Macromol Symp. 1999,144:391.
[121] Majeti N V, Kumar R. Reactive&Functional Polymers. 2000,46:1.
[122] Hall L.D,Yalpani M. Biopolymers. 1981,20:1413.
[123] Rudall K.M.Adv Insect Physiol. 1963,(1):257.
[124] 蒋挺大．《甲壳素》[M]．北京：中国环境科学出版社．1999．
[125] Kuirta K, Sannan T, Iwakura Y.Makromol Chem. 1977(178):3197.
[126] 徐健，金鑫荣．大学化学．1994(3)：22．
[127] 奇云，杜润才．现代化工．1995 (1)：15．
[128] Kotze A F, Thanon M M,Luebetane H H,et al. J.Pharm Sci. 1999,88(2):253.
[129] Ueno H,Yamada H,Tanaka I,et al. Biomaterials. 1999,20:1407.
[130] 张沈丽，王丽娟，肖庆恒等．中国海洋药物．2000,(1)：11．
[131] Khairoun I, Deriessens F C M,Boltong M G, et al. Biomaterials. 1999,20(4):393.
[132] 康站燕，耿丽华，李数品．山东科学．1999，12(1)：55．
[133] Kweon D K, Kang D W. J Appl Polym Sci. 1999,74:458.
[134] 严俊．化学通报．1984，47(11)：26．
[135] Kurita K.Polym Degradation and Stability. 1998,59:117.
[136] 俞继华，冯才旺，唐有根．广西化工．1997，26(3)：28．
[137] 蒋挺大．工业微生物．1998，28(3)：32．
[138] Stanley, W,L,Watters,G,G.Kelly, S.H.and Olson.A.C. Biotechnol.Bioeng. 1978,20:135
[139] Stanley, W,L, Watters, G,G. Chan, b.and Mercer, J.M. Biotechnol. Bioeng. 1975,17:315.
[140] Liu, W.H.Wang, S.D and Su, Y.C. Proc. Natl. Sci. Council(Tatwan). 1978,2:275.
[141] 徐风彩等．生物化学杂志．1992，8(5)：27．
[142] Iyengar, L.and Rao,A.V.S.P, Biotechnol, Bioeng. 1979,21:1333.
[143] Tsumura, N. Kasumu,T. and Ishikawa, M. shokukin Sogo Kenk. Hokoku. 1976,31:71.
[144] Leuba, J .L. and Widmer, F, Biotechnol. Lett. 1979,1:109.
[145] 侯建明等．中国医药工业杂志．1993，24(3)：100．
[146] 陈盛，吴海生．生物化学与生物物理进展．1990，17(5)：383．
[147] 陈盛，黄智跃，刘艳如．生物化学与生物物理进展．1996，23(3)：250．
[148] Isowa, Y;Ohmori, M;Ichikawa, T;Kurita, H. Bull. Chem. Soc. Jpn. 1977,50(10):2762.
[149] Isowa, Y, et al. Tetrahedron Lett. 1979:2611.
[150] Tsumura, N. Kasumu, T. and Ishikawa, M, Starch/Saerke. 1978,30:420.
[151] Wang Jianlong .Qian Yi. Chemosphere. 1999, 38(13):3109.
[152] Lee, K.H.J. Chem. Technol. Biotechnol. 1992,54(2):375.
[153] Hirano S. Progr. Biotechnol. 1987,(3):163.
[2] 杨智宽，韦进宝．<<污染控制化学>[M]．湖北：武汉大学出版社．1998：402．
[3] 张锦，李圭白，马军．化学工程师．2001，(2)：36．
[4] 黄志勇，陈国树．环境与开发．1997，12(2)：32．
[5] 林齐平．水处理技术．1993，19(2)：174．
[6] 乌锡康．<<有机废水处理>．[M]北京：化学工业出版社．1999：147．
[7] 张芳丙，金承基等．<<含酚废水的处理与利用>[M]．北京：化学工业出版社．1983．
[8] 张全兴，刘天华．化工环保．1994，14：344．
[9] Magne P.and Walker EL. Carbon. 1986,24(2):101.
[10] 戴蝤元，杨义燕，杨天雪．化工进展．1991，6：40．
[11] 孙贤波，杜慧芳．环境化学．1999，18(1)：46．
[12] 潘碌亭，肖锦．膜科学与技术．1999，19(5)：19．
[13] 曾炳蔚，郑景涛．化工时刊．1997，11(7)：18．
[14] Yinhua Wan,Xiangde Wang,Xiujuan Zhang. J Membrane Science. 1997,135 (2): 263.
[15] Jiri S Sb Praci Vyzkumn Chem,Vynziti Uhli, Dehtu Ropy.No.1992,2: 397.
[16] 唐守映等．环境科学研究．1995，8(6)：37．
[17] 王检．环境工程．1994，12(6)：16．
[18] Zumbrunn J P, Crommelynck F.Ger, Offen. 1990,222853.
[19] 刘勇弟等．中国环境科学．1994，14(5)：341．
[20] Rajko K Tehnika. 1959,14,008f～200k..
[21] Mikhailova M et al. sb Dokl, -Natskonf. Mladite Nauchni Rab. Spets."Neft Khim",1st,1976;327.
[22] Arai shigeru et al.Japan.Kokai. 1975,74 44554.
[23] Kharlamova T A,gorkhova LT.Khim. Yekhnol Vody. 1982,4(2):157.
[24] Fiti M .Report, 1975 AED-CONF 75-061-015.
[25] 杨琦，单立志，钱易．环境科学研究．1998，11 (4)：62．
[26] Hu,X.,Lei.1.,Chen,G and Yue,P.L. Water Res .2001,35(8):2078.
[27] Li L,et al. J AICHE,1991,37(11):1678.
[28] Barner H.E.,Huang C.Y., Johnson T.et al. J of Hazardous Materials. 1992,31:1.
[29] 赵金辉，张克荣等．中国卫生检验杂志．2000，10 (1)：5．
[30] Mario Schiavello, Kluwer Academic Publishers. 1998.
[31] R.Bauer ,G.Waldner. Catalysis Today. 1999,53:131.
[32] Lei,L.,Hu,X.et al. J of Photochemistry and Photobiology A-Chemistry.1998,16:159.
[33] 胡春，王怡中．环境科学．1997，18(4)：1．
[34] 魏宏斌．中国给水排水．1999，15(2)：14．
[35] 陈英，钟理．化工进展．1999，18(5)：26．
[36] 王建龙，施汉昌，钱易．环境科学研究．1999，12(1)：60．
[37] Si-Jing Wang,Kai-Chee Loh. Biotechnol.Bioeng.2000,68(4):437.
[38] Kira.A.Onysko,HectorM.Budman,CampbellW.Robinson.Biotechnol.Bioeng.2000,70(3):291.
[39] Garcia I.,Bonilla Venceslada J.L.,et al. Water Res. 1997,31(5):2005.
[40] Conner M.A.and Allgeier R.J.Adv. Appl. Microbiol. 1976,28:878.
[41] Chibata Z. and Tosa T. Appl. Biochem. Bioeng. 1983,4:1.
[42] Scott C.D. Enzyme Microb. Technol. 1987,9;66
[43] 王建龙著．《生物固定化技术与水污染控制》[M]．北京：科学出版社．2002．
[44] M Fujimura et al. Appl Microbiol Biotechnol. 1984,19(1):79.
[45] I S Maddox et al. Biotechnol. Bioeng. 1981,23(1):349.
[46] O S Inloes et al. Biotechnol. Bioeng. 1983,25(5):2653.
[47] Grubhofer N. And Schleith L. Naturwissenschafier. 1953,40:508.
[48] Campbell D. H. Luescher E. and Lerman, L.S Proc. Natl.Acad.Sci,USA, 1951,37:575
[49] Tosa T, Mori T, Fuse N. And Chibata I, Enzymolgia. 1966,31;214.
[50] Nunez M.J and Lema J.M. Enzyme Microb. Technol. 1987,9:642.
[51] Bimbaum S, Larsson, P. O. Nisbach, K. 1986, Pergamon Press, Elmsford, New York:45.
[52] 千佃一郎等．《固定化酵素》[M]．论坛社，东京．1975．
[53] 居乃琥，全国固定化生物催化剂学术讨论会报告．1988．
[54] Arcuri E.J et al .Biotechnol. Bioeng. 1982,14:593.
[55] 居乃琥．食品与发酵工业．1981，No．6：30．
[56] Baker D A,et al, J. Inst. Brew.1978,79:487.
[57] Kolot F. B. Process Biochemistry. 1980,15(7):2.
[58] Kinner N.E. and Eighmy, T.T,J.Water Pollut, Control Fed. 1985,57:526.
[59] Bidey s.p, In immobilized cells and enzymes, a practical Approach (Woodward, J,ed),CRL Press, Oxford. 1985,147.
[60] 居乃琥等编著．《固定化酶理论与应用．》[M]．北京：轻工业出版社．1987：169．
[61] Cabral,J.M.S,et al.Ann. N. Y.Acad. Sci. 1984,434:483.
[62] Van Haecht J.L.et al. Biotechnol, Bioeng. 1985,27:217.
[63] Krug T.A,and Dauglis A.J,Biotechnol. Lett. 1983,5:159.
[64] Gainer J.L.and Kirwan D.J. Ann, N,Y, Acad, Sci. 1984,434:465.
[65] Daugulis A.J,et al, Biotech. Bioeng. 1985,27:626.
[66] Okita W.B.,et al., Biotechnol. Bioeng. 1985,27:634.
[67] Chibata I. Et al, Appl. Microbiol. 1974,27:878.
[68] Snell R.L.,U.S.Patent. 1976,No.3:974.
[69] Mohan R.R. and Li N.N., Biotechnol. Bioeng. 1975,17:1137.
[70] Lim F. and Sun A.M., Science. 1980,210:908.
[71] 居乃琥．工业微生物．1989，199(1)：25．
[72] Cavin J .F.,et al.,Biotechnol. Lett, 1985,7:821.
[73] Linko P.,et al.,Ann N.Y.Acad. Sci. 1984,434:406.
[74] Loung J.H.,et al.,Appl. Microbiol. Biotechnol. 1984,19:207.
[75] Chibata I.,et al. In Manual of Industrial Microbiology and Biotechnology American Society for Microbilogy, Washington, D.C. 1986,217.
[76] Takamatsu S.,et al., Eur. J. Appl. Microbiol. Biotechnol. 1982,15:147.
[77] Bang W.G., et al., Biotechnol. Bioeng. 1983,25:1013.
[78] Fujimura M., et al., Appl. Microbiol. Biotechnol. 1984,19:136.
[79] O shea G.M.,et al., Biochim. Biophys Acta. 1984,804:133.
[80] Goosen M. F.A., et al., Biotechnol. Bioeng. 1985,27:146.
[81] 居乃琥．生物工程研究进展．1987，No5，1．
[82] Mcloughlin A.J., Int. Indust .Biotechnol. 1986,6:180.
[83] Congress of the United states. OTA Report on Biotechnology, Commercial Biotechnology. 1984.
[84] 田中渥夫，福井三郎．化学工学．1978，43：269．
[85] Tanaka.A.,et al., Eur. J.Appl. Microbiol. Biotechnol. 1978,5:17.
[86] H Bettmann et al. Appl. Microbiol. Biotechnol. 1984,20:285.
[87] H Bettmann et al. Appl.Microbiol. Biotechnol. 1985,22:289.
[88] F. Westmeier et al.Appl.Microbiol. Biotechnol. 1985,22:301.
[89] 周相林等．环境科学学报．1986，6(3)：368．
[90] 陈陶声．《固定化酶理论与应用》[M]．北京：轻工业出版社．1987．
[91] 黄武华等．环境科学学报．1982，2(4)：293．
[92] A M Anselmo et al．应用微生物．1987，(3)：59．
[93] H M Ehrhardt et al. Appl Microbiol Biotechnol.1989,30(3):312.
[94] 孙艳，李京，谭立扬．环境科学研究．1999，12(1)：1．
[95] Sofer, S.S.J Water Pollut. Control Feed. 1990,62(1):73.
[96] K T O Reilly. et al. Appl. Environ. Microbiol. 1996,55(9):2113.
[97] K T O Reilly et al. In:IAWPRC conference on water and wastewater microbiology. Newport Beach, CA,U.S.A. 1996,8.
[98] 蒋文锐等．环境科学．1981，2(3)：196．
[99] 杨连栋．水处理技术．1990，16(3)：223．
[100] J P Kutney et al. Appl. Environ. Microbiol. 1988,54(4):1015.
[101] J Beuuink et al. Appl Microbiol Biotechnol. 1988,29(1):72.
[102] B Bisping et al. Biotechnol. Appl. Biochem. 1988,10 (2):87.
[103] H J Gijen et al. Biotechnol. Lett. 1988,10(1):61.
[104] L E Macaskie et al. Biotechnol. Bioeng. 1987,30(1):66.
[105] Geoffrey W Garnham, Geoffrey A Codd, Geoffrey M Gadd. Environ. Sci. Technol. 1992,26:1764.
[106] 胡庆晏等．环境污染与防治．1993，25(1)：35．
[107] 黄晓维，王春生，王孔星等工业水处理．1989，9(5)：23．
[108] 梁沈平，王菊思．环境科学．1998，19(4)：13．
[109] 王孔星，张秀文，谢裕敏等．环境科学与技术．1990，2(2)：2．
[110] 刘志培，杨惠芳，贾省芳等．环境科学．1992，13(1)：2．
[111] Livernoche, D, et al., Biotechnol. Bioeng. 1983,25(9):2055.
[112] S. Ohmomo, et al., Agric. Biol. Chem. 1988,52(10):2437.
[113] 蒋挺大．《壳聚糖》[M]．北京：化学工业出版社．2001．
[114] Stegemann H.Z.Physiol. Chem. 1963,331:269.
[115] Herzog K H,Grossmann H,Liefiaender M.Z.Physiol .Chem. 1975,356:1067.
[116] Surarit R, Gopel P K,Shepherd M G. J.Gem,Microbiol. 1988,134:1723.
[117] 倪红，杨艳红，陈怀新．湖北大学学报 (自科版)．2001(1)：77．
[118] Chuang W Y, Young T H,Yao C H,et al. Biomaterials. 1999,20:1479.
[119] Koyano T, Koshizaki N,Umehara H, et al. Polymer. 2000,41:4461.
[120] Mucha M,Piekielna J, Wieczorek A. Macromol Symp. 1999,144:391.
[121] Majeti N V, Kumar R. Reactive&Functional Polymers. 2000,46:1.
[122] Hall L.D,Yalpani M. Biopolymers. 1981,20:1413.
[123] Rudall K.M.Adv Insect Physiol. 1963,(1):257.
[124] 蒋挺大．《甲壳素》[M]．北京：中国环境科学出版社．1999．
[125] Kuirta K, Sannan T, Iwakura Y.Makromol Chem. 1977(178):3197.
[126] 徐健，金鑫荣．大学化学．1994(3)：22．
[127] 奇云，杜润才．现代化工．1995 (1)：15．
[128] Kotze A F, Thanon M M,Luebetane H H,et al. J.Pharm Sci. 1999,88(2):253.
[129] Ueno H,Yamada H,Tanaka I,et al. Biomaterials. 1999,20:1407.
[130] 张沈丽，王丽娟，肖庆恒等．中国海洋药物．2000,(1)：11．
[131] Khairoun I, Deriessens F C M,Boltong M G, et al. Biomaterials. 1999,20(4):393.
[132] 康站燕，耿丽华，李数品．山东科学．1999，12(1)：55．
[133] Kweon D K, Kang D W. J Appl Polym Sci. 1999,74:458.
[134] 严俊．化学通报．1984，47(11)：26．
[135] Kurita K.Polym Degradation and Stability. 1998,59:117.
[136] 俞继华，冯才旺，唐有根．广西化工．1997，26(3)：28．
[137] 蒋挺大．工业微生物．1998，28(3)：32．
[138] Stanley, W,L,Watters,G,G.Kelly, S.H.and Olson.A.C. Biotechnol.Bioeng. 1978,20:135
[139] Stanley, W,L, Watters, G,G. Chan, b.and Mercer, J.M. Biotechnol. Bioeng. 1975,17:315.
[140] Liu, W.H.Wang, S.D and Su, Y.C. Proc. Natl. Sci. Council(Tatwan). 1978,2:275.
[141] 徐风彩等．生物化学杂志．1992，8(5)：27．
[142] Iyengar, L.and Rao,A.V.S.P, Biotechnol, Bioeng. 1979,21:1333.
[143] Tsumura, N. Kasumu,T. and Ishikawa, M. shokukin Sogo Kenk. Hokoku. 1976,31:71.
[144] Leuba, J .L. and Widmer, F, Biotechnol. Lett. 1979,1:109.
[145] 侯建明等．中国医药工业杂志．1993，24(3)：100．
[146] 陈盛，吴海生．生物化学与生物物理进展．1990，17(5)：383．
[147] 陈盛，黄智跃，刘艳如．生物化学与生物物理进展．1996，23(3)：250．
[148] Isowa, Y;Ohmori, M;Ichikawa, T;Kurita, H. Bull. Chem. Soc. Jpn. 1977,50(10):2762.
[149] Isowa, Y, et al. Tetrahedron Lett. 1979:2611.
[150] Tsumura, N. Kasumu, T. and Ishikawa, M, Starch/Saerke. 1978,30:420.
[151] Wang Jianlong .Qian Yi. Chemosphere. 1999, 38(13):3109.
[152] Lee, K.H.J. Chem. Technol. Biotechnol. 1992,54(2):375.
[153] Hirano S. Progr. Biotechnol. 1987,(3):163.