构建带内支撑的组织工程化软骨的实验研究
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摘要
本研究旨在探讨构建一种具有预制体积与形状的带有内支撑的组织工程化软骨的方法及其可行性。利用不同的培养模式(体外、裸鼠体内、兔体内)依次构建带内支撑组织工程化软骨并观察其特点。并先后于裸鼠皮下、兔再造阴茎模型内及兔阴囊原位构建组织工程化的阴茎及睾丸假体并观察其特点。结果证实:1)利用软骨细胞与PGA支架构建的组织工程化软骨存在“空心”现象。2)利用软骨细胞与HDPE-PGA支架可以于裸鼠体内构建出具有预制体积与形状、组织学良好的HDPE-软骨复合体。3)软骨细胞与HDPE-PGA支架复合物自体移植后,可构建出具有预制体积与形状、结构良好的HDPE-纤维软骨复合体;延长体外时间至4周有利于改善新生软骨的质量。4)即时再造阴茎内与皮下具有相同的构建组织工程化软骨的内环境。5)利用此方法,亦可以成功构建出组织工程化阴茎及睾丸假体。
The aim was to investigate the feasibility of creating tissue-engineered cartilage in predetermined size and shape. Tissue-engineered cartilage with internal support was created in vitro or in vivo, tissue-engineered penis and testis prostheses were created gradually in nude mice and rabbit models. Results showed that: 1)“Hollow”phenomenon occurs in tissue-engineered cartilage when PGA scaffold is configured into thicker size. 2) Using PGA scaffold with internal porous HDPE support, tissue-engineered cartilage in predetermined shape and size can be created in vivo.3) In immunocompetent animals, PGA generates an inflammatory reaction that results in creation of fibrocartilage, elongating the time of cultivation in vitro is conducive to the formation of tissue- engineered cartilage.4) Newly reconstructed penis has the same internal environment as subcutaneous space for creation of tissue-engineered cartilage 5) Tissue-engineered penis and testis prostheses can also be created successfully.
The aim was to investigate the feasibility of creating tissue-engineered cartilage in predetermined size and shape. Tissue-engineered cartilage with internal support was created in vitro or in vivo, tissue-engineered penis and testis prostheses were created gradually in nude mice and rabbit models. Results showed that: 1)“Hollow”phenomenon occurs in tissue-engineered cartilage when PGA scaffold is configured into thicker size. 2) Using PGA scaffold with internal porous HDPE support, tissue-engineered cartilage in predetermined shape and size can be created in vivo.3) In immunocompetent animals, PGA generates an inflammatory reaction that results in creation of fibrocartilage, elongating the time of cultivation in vitro is conducive to the formation of tissue- engineered cartilage.4) Newly reconstructed penis has the same internal environment as subcutaneous space for creation of tissue-engineered cartilage 5) Tissue-engineered penis and testis prostheses can also be created successfully.
引文
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