老年肺癌患者血清和尿液中碱性成纤维细胞生长因子的检测及意义
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摘要
本研究采用酶联免疫吸附法检测了30例老年肺癌患者血清和尿液中碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)的表达及含量情况,研究老年肺癌患者血清和尿液中bFGF的表达及二者的相关关系、它们与肺癌的分化程度、临床分期等的关系。
结果表明:1、肺癌患者血清中bFGF的浓度高于对照组(p<0.05),并与肺癌的恶性程度、分期相关。2、肺癌患者尿液中bFGF的浓度高于对照组(p<0.05),且与肺癌的恶性程度、分期相关。3、肺癌患者血清bFGF值与尿液bFGF值的关系通过Spearman等级相关分析,发现两者存在正相关(rs=0.9971,P<0.01)。
结论:1、老年肺癌患者血清和尿液中的bFGF的水平明显增高。2、老年肺癌患者血清和尿液中的bFGF的水平与肺癌侵袭性、临床分期、不良预后等有密切关系,它们的检测可作为指导临床治疗的有价值的指标。3、老年肺癌患者血清和尿液中bFGF呈正相关,尿液bFGF水平可以反映血中bFGF水平,尿液bFGF有望成为老年肺癌患者的一项无创检查指标。
In the study we evaluated the expression and the concentrations of basic fibroblast growth factor (bFGF) in the serum and urine. ELISA was used to detect the concentration of bFGF in the serum and urine of 30 lung cancer patients and the relationship between them ,then explore their relationship with the degree of tumor differentiation, clinical stage of lung cancer.
The results demonstrated as below: 1、The concentrations of bFGF in the sera were higher in patients with lung cancer then those in the controls(p<0.05) ,and correlated with the clinical stages. 2、The concentrations of bFGF in the urine were also higher in patients with lung cancer then those in the controls (p<0.05),and correlated with the clinical stages. 3、The concentrations of bFGF in the urine and them in the sera were positive correlation.
The following conclusions were drawd: 1、The concentrations of both serum and urinary bFGF are markedly high.2、Both serum and urinary bFGF are clinical relevant with lung cancer,they are correlated to the aggression and development of the lung cancer,the assay of serum and urinry bFGF is helpful in predicting the risk for metastasis and prognosis in lung cancer.3.、The serum and urinary bFGF were positive correlation.,urinary bFGF can reflex the concentrations of bFGF in the serum,and will be an atraumatic detection index for the elder lung cancer patients.
结果表明:1、肺癌患者血清中bFGF的浓度高于对照组(p<0.05),并与肺癌的恶性程度、分期相关。2、肺癌患者尿液中bFGF的浓度高于对照组(p<0.05),且与肺癌的恶性程度、分期相关。3、肺癌患者血清bFGF值与尿液bFGF值的关系通过Spearman等级相关分析,发现两者存在正相关(rs=0.9971,P<0.01)。
结论:1、老年肺癌患者血清和尿液中的bFGF的水平明显增高。2、老年肺癌患者血清和尿液中的bFGF的水平与肺癌侵袭性、临床分期、不良预后等有密切关系,它们的检测可作为指导临床治疗的有价值的指标。3、老年肺癌患者血清和尿液中bFGF呈正相关,尿液bFGF水平可以反映血中bFGF水平,尿液bFGF有望成为老年肺癌患者的一项无创检查指标。
In the study we evaluated the expression and the concentrations of basic fibroblast growth factor (bFGF) in the serum and urine. ELISA was used to detect the concentration of bFGF in the serum and urine of 30 lung cancer patients and the relationship between them ,then explore their relationship with the degree of tumor differentiation, clinical stage of lung cancer.
The results demonstrated as below: 1、The concentrations of bFGF in the sera were higher in patients with lung cancer then those in the controls(p<0.05) ,and correlated with the clinical stages. 2、The concentrations of bFGF in the urine were also higher in patients with lung cancer then those in the controls (p<0.05),and correlated with the clinical stages. 3、The concentrations of bFGF in the urine and them in the sera were positive correlation.
The following conclusions were drawd: 1、The concentrations of both serum and urinary bFGF are markedly high.2、Both serum and urinary bFGF are clinical relevant with lung cancer,they are correlated to the aggression and development of the lung cancer,the assay of serum and urinry bFGF is helpful in predicting the risk for metastasis and prognosis in lung cancer.3.、The serum and urinary bFGF were positive correlation.,urinary bFGF can reflex the concentrations of bFGF in the serum,and will be an atraumatic detection index for the elder lung cancer patients.
引文
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2、Gospodarowicz D, Greenburg G, Bialecki H, et al. Factors involved in the modulation of cell proliferation in vivo and in vitro: the role of fibroblast and epidermal growth factors in the proliferative response of mammalian cells. In Vitro,1978, 14(1) :85-118.
3、Okada-Ban M,Thiery J P,Jouanneau J,et al.Fibroblast growth factor-2[J].Int Biochem,2000,32(3):263-267.
4、Baird A ,Walicke P A.Fibroblast growth factors.Br Med Bull,1989;45(2):438-452.
5、叶安霞,谢正福.碱性成纤维细胞因子在肺病的研究进展[J]. 国际呼吸杂志,2006;26(7):499-502.
6、Esch F, Ueno N, Baird A, et al. Primary structure of bovine brain acidic fibroblast growth factor (FGF). Biochem Biophys Res Common,1985,133(2):554-562.
7、Baird A ,Walicke P A.Fibroblast growth factors.Br Med Bull,1989,45(2):438-452.
8.Doniach T.Basic FGF as an inducer of anteroposterior neural pattern. Cel1,1995,83(7) :1067-1070.
9、Mason I J. The ins and outs of Fibroblast growth factors. Cell,1994,78(4);547-552.
10、Ono I.Roles of cytokines in wound healing processes[J].Nippon Geka Gakkai,Zasshi,1999;100(9):522-528
11、Brew E C,Mitchell M B,Harken A H,et al.Fibroblast growth factors in operative wound healing.Am Coll Surg.1995;180(4):499-504.
12、Ernst H,Konturek PC,Hahn EG,el al.Effect of local injection with basic fibroblast growth factor (bFGF) and neurtralizing antibody to (bFGF) on gastric ulcer healing,gastric secretion,angiogenesis and gastric blood flow.J Physiol Pharmacol,2001;52(3):377.
13、Palmer TD,Markakis EA,Willhoite A R et al.Fibroblast growth factor-2 activase latent neurogenic program in neural stem cells from diverse regions of the adult CN S[J].Neuro sci,1999;19(19):8487-8497.
14、管英俊,高英茂,李 洁 et al.BFGF、NGF、EGF及受体在人胚神经管早期发育中的表达[J].中国组织化学与细胞生物学杂志,2000;9(2):126-129.
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21、李艳,王新允,刘婷,等.组织芯片技术研究肺癌及癌前病变bFGF的表达和意义[J].肿瘤防治杂志,2005,12(9):668-671.
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23、蔡欣然,黄长玉,杨发端,等.人肝胆管癌肿中碱性成纤维细胞生长因子及其受体的表达[J].中华实验外科杂志,2000;17(1):82.
24、钟华,邓方明,孔祥田,等. 碱性成纤维细胞生长因子及其受体在肾癌组织中的表达[J].中华外科杂志,1996,34(11):651.
25、石歧兴,李方,刘同才,等.VEGF和bFGF共同表达与膀胱癌生物学行为的关系[J].实用肿瘤杂志,2001,16(1):30.
26、邓志宏,黄维国,邱建华,等.喉粘膜癌前病变和癌变中VEGF、bFGF和KGF的表达分析[J].肿瘤,1999,19(6):338.
27、胡波,贾永华,李小薇.碱性纤维母细胞生长因子(bFGF)的检测有助于判断肉瘤恶性程度[J].大连医科大学学报,2001,23(1):24.
28、林卫,彭芝兰.碱性成纤维细胞生长因子在卵巢上皮性肿瘤中的表达及意义[J].中国修复重建外科杂志,1999,13(5):299.
29、李玲,刘毅智,吴宜林,等.卵巢恶性肿瘤VEGFmRNA和bFGFmRNA的表达及意义[J].湖南医科大学学报,2000,25(4):406.
30、Takahashi K.Mulliken JB.The cellular marks that distinguish the phase of hemangioma during infancy and childhood[J].J Clin Invest.1994,93(6):2357-2364.
31、汪 伟,丁 强,张元芳,等.膀胱肿瘤手术前后血清和尿液bFGF浓度监测的临床意义[J].中华泌尿外科杂志,2004,25(4):27.
32、石歧兴,李方,刘同才,等.膀胱移行细胞癌中碱性成纤维细胞生长因子表达的意义[J].实用癌症杂志,2000,15(4):394-396.
33、Inoue K,Perrotte P,Wood CG,et al.Gene therapy of human bladder cancer with adenovirus-mediated antisense basic fibroblast growth factor[J].Clin Cancer Res,2000,6(11):4422-4431.
34、李四平,刘叙仪,王洁,等.反应停对人肺腺癌亲代及耐顺铂细胞系碱性成纤维细胞生长因子表达影响.中国呼吸与危重监护杂志,2003,2(1):40-43.
35、余红娥,余清声,刘新艳,等.中华眼镜蛇毒组分抑制肺癌细胞H1299的血管生长因子VEGF和bFGF.中国临床药理学与治疗学,2005,10(9):970-973.
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65、姜昊文,张元芳,丁强,等.血清和尿液的碱性成纤维细胞生长因子诊断膀胱肿瘤的临床意义.上海医学.2004,27(5):312-313.
2、Gospodarowicz D, Greenburg G, Bialecki H, et al. Factors involved in the modulation of cell proliferation in vivo and in vitro: the role of fibroblast and epidermal growth factors in the proliferative response of mammalian cells. In Vitro,1978, 14(1) :85-118.
3、Okada-Ban M,Thiery J P,Jouanneau J,et al.Fibroblast growth factor-2[J].Int Biochem,2000,32(3):263-267.
4、Baird A ,Walicke P A.Fibroblast growth factors.Br Med Bull,1989;45(2):438-452.
5、叶安霞,谢正福.碱性成纤维细胞因子在肺病的研究进展[J]. 国际呼吸杂志,2006;26(7):499-502.
6、Esch F, Ueno N, Baird A, et al. Primary structure of bovine brain acidic fibroblast growth factor (FGF). Biochem Biophys Res Common,1985,133(2):554-562.
7、Baird A ,Walicke P A.Fibroblast growth factors.Br Med Bull,1989,45(2):438-452.
8.Doniach T.Basic FGF as an inducer of anteroposterior neural pattern. Cel1,1995,83(7) :1067-1070.
9、Mason I J. The ins and outs of Fibroblast growth factors. Cell,1994,78(4);547-552.
10、Ono I.Roles of cytokines in wound healing processes[J].Nippon Geka Gakkai,Zasshi,1999;100(9):522-528
11、Brew E C,Mitchell M B,Harken A H,et al.Fibroblast growth factors in operative wound healing.Am Coll Surg.1995;180(4):499-504.
12、Ernst H,Konturek PC,Hahn EG,el al.Effect of local injection with basic fibroblast growth factor (bFGF) and neurtralizing antibody to (bFGF) on gastric ulcer healing,gastric secretion,angiogenesis and gastric blood flow.J Physiol Pharmacol,2001;52(3):377.
13、Palmer TD,Markakis EA,Willhoite A R et al.Fibroblast growth factor-2 activase latent neurogenic program in neural stem cells from diverse regions of the adult CN S[J].Neuro sci,1999;19(19):8487-8497.
14、管英俊,高英茂,李 洁 et al.BFGF、NGF、EGF及受体在人胚神经管早期发育中的表达[J].中国组织化学与细胞生物学杂志,2000;9(2):126-129.
15、Walicke PA,Baird A.Neurotrophic effects of basic and acidic fibroblast growth factors are not mediated through glial cells[J].Brain Res,1998;40(8):71-79.
16、唐孝明,裴福兴,谭健三.碱性成纤维细胞生长因子促神经再生的实验研究[J].中华显微外科杂志,1998;26(12):2029-2036.
17、Smith X,Fox S B,Whitehouse K et al.Upregulation of basic fibroblast growth factor in breast carcinoma and its relationship to vascular density,oestrogen receptor,epidermal growth factor receptor and survival[J].Ann Oncol,1999;10(6):707-713.
18、Davles M M,Jonas S K,Kaur S,et al.Plasma vascular endothellal but fibroblast growth factor levels correlate with colorectal liver metastasls vascularlty and volume[J].Br J Canoer,2000,82(5):1004-1008.
19、Takanami I,Tanaka F,Hashizume T,et al.Tumor angiogenesis in plumonary adenocarcinomas:relationship with basic fibroblast growth factor,its receptor,and survival[J].Neoplasma,1997;44(5):295-298.
20、Slodkowska J,Sikora J,Roszkowski-Sliz,et al.Expression of vascular endothelial growth factor and basic fibroblast growth factor receptors in lung cancer.Analytical and Quantitative Cytology and Histology,2000,22(5):398-402.
21、李艳,王新允,刘婷,等.组织芯片技术研究肺癌及癌前病变bFGF的表达和意义[J].肿瘤防治杂志,2005,12(9):668-671.
22、Noda M,Hatton T,Kimura T,et al.Expression of fibroblast growth factor 2 mRNA in early and advanced gastric cancer[J].Acta Oncol,1997;36(7):695-700 .
23、蔡欣然,黄长玉,杨发端,等.人肝胆管癌肿中碱性成纤维细胞生长因子及其受体的表达[J].中华实验外科杂志,2000;17(1):82.
24、钟华,邓方明,孔祥田,等. 碱性成纤维细胞生长因子及其受体在肾癌组织中的表达[J].中华外科杂志,1996,34(11):651.
25、石歧兴,李方,刘同才,等.VEGF和bFGF共同表达与膀胱癌生物学行为的关系[J].实用肿瘤杂志,2001,16(1):30.
26、邓志宏,黄维国,邱建华,等.喉粘膜癌前病变和癌变中VEGF、bFGF和KGF的表达分析[J].肿瘤,1999,19(6):338.
27、胡波,贾永华,李小薇.碱性纤维母细胞生长因子(bFGF)的检测有助于判断肉瘤恶性程度[J].大连医科大学学报,2001,23(1):24.
28、林卫,彭芝兰.碱性成纤维细胞生长因子在卵巢上皮性肿瘤中的表达及意义[J].中国修复重建外科杂志,1999,13(5):299.
29、李玲,刘毅智,吴宜林,等.卵巢恶性肿瘤VEGFmRNA和bFGFmRNA的表达及意义[J].湖南医科大学学报,2000,25(4):406.
30、Takahashi K.Mulliken JB.The cellular marks that distinguish the phase of hemangioma during infancy and childhood[J].J Clin Invest.1994,93(6):2357-2364.
31、汪 伟,丁 强,张元芳,等.膀胱肿瘤手术前后血清和尿液bFGF浓度监测的临床意义[J].中华泌尿外科杂志,2004,25(4):27.
32、石歧兴,李方,刘同才,等.膀胱移行细胞癌中碱性成纤维细胞生长因子表达的意义[J].实用癌症杂志,2000,15(4):394-396.
33、Inoue K,Perrotte P,Wood CG,et al.Gene therapy of human bladder cancer with adenovirus-mediated antisense basic fibroblast growth factor[J].Clin Cancer Res,2000,6(11):4422-4431.
34、李四平,刘叙仪,王洁,等.反应停对人肺腺癌亲代及耐顺铂细胞系碱性成纤维细胞生长因子表达影响.中国呼吸与危重监护杂志,2003,2(1):40-43.
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