有氧运动及标准饲料对胰岛素抵抗大鼠脂质代谢的影响
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摘要
研究目的:观察有氧运动对高脂高糖饲料喂养形成胰岛素抵抗大鼠脂质代谢的影响。
研究方法:选用清洁级标准健康雄性SD大鼠215只,7周龄,206-257g,适应性训练一周后,分为A.安静对照组(CON)15只,B.胰岛素抵抗模型组200只,测量空腹血糖,放射免疫法测血胰岛素,计算胰岛素敏感指数,确定胰岛素抵抗大鼠模型的建立。建模成功后分为四组:高脂膳食组(DIO)、高脂膳食运动组(DE)、标准饲料组(NOR)、标准饲料运动组(NE)。八周干预后,各运动组大鼠于末次运动训练后和各安静组,于第二天早晨水合氯醛腹腔麻醉,取心脏和肝脏,置于-80°C冰箱保存。western blot半定量测定PPAR-γ表达;HE染色定性分析肝组织和心肌细胞的形态学变化,油红O染色半定量分析肝组织脂质的变化和定性分析心肌脂质的变化。
研究结果:1.胰岛素抵抗组大鼠胰岛素敏感指数明显低于对照组。通过有氧运动或/和标准饲料,胰岛素抵抗大鼠胰岛素敏感指数显著升高。
2.肝组织的脂质出现油红O明显着色:深红色。安静对照组大鼠的肝组织着色均匀地分散开来,而高脂组大鼠的肝组织着色明显,并且着色加深,着色面积明显增大;进行有氧运动和/或标准饲料后,干预组大鼠的肝组织与高脂组大鼠肝组织相比,着色面积明显减少;其中标准饲料组大鼠和标准饲料运动组大鼠的肝组织着色状况,与安静对照组大鼠的肝组织相比,已趋于正常;高脂膳食运动组大鼠的肝组织着色面积与高脂膳食组大鼠相比,显著性减少。
3.大鼠心肌的PPAR-γ经干预后的变化:与安静对照组(CON)相比,高脂膳食+运动组(DE)和标准饲料+运动组(NE)大鼠心肌的PPAR-γ的表达量明显增高(P<0.05),而高脂膳食组(DIO)大鼠心肌的PPAR-γ的表达量明显降低(P<0.05);与高脂膳食组(DIO)大鼠相比,干预三组的PPAR-γ均有所增高,其中高脂膳食+运动组(DE)和标准饲料+运动组(NE)大鼠心肌的PPAR-γ的表达量明显上升(P<0.05),而标准饲料组(NOR)虽有所增高,但无统计学意义(P>0.05)。
研究结论:1.连续8周的高脂高糖膳食能建立胰岛素抵抗大鼠模型。2.有氧运动或/和标准饲料均可改善胰岛素抵抗中脂质代谢紊乱,改善胰岛素抵抗状态。
Obejective: To observe the effect of aerobic exercise and standard fodder which has affected the lipid metabolism of the insulin resistance rats which was fed by the fatty and high sugar .
Methods:To select 215 clean and healthy male Sprague-Dawley(SD)(purchased from B&K Universal Group Limited )which were 7 weeks age and weighted from 206g to 257g,after adaptive training for a week,the paper divided them into 2 groups stochastically:A、Control,C,n=15,B、Model of Insulin Resistance,MIR,n=200.After buliding the model of IR rats, then divided into 4 groups of MIR,were high fat diet control group(DIO),high fat diet exercise group(DE),ordinary diet group(NOR) and ordinary diet exercise group(NE). Exercise 8 weeks.Measure the blood glucose immediately and the insulin by radioimmunoassay,calculate insulin sensitivity index to determine the model of the insulin resistance in the rat established.
After the high fat diet exercise group and ordinary diet exercise group finished its last exercises training,in the next morning the each groups rats were given a general anesthesia with chloralhydrate injection into the abdominal cavity,then were killed. Take the heart of the rat to the fridge of -80°c . Analyze the PPAR-γin cardiac muscle by western blot quantificationally, analyze the morphological changes of the hepar and myocardial cells qualitatively,analyze the adipose change of the hepar and myocardial cells by Oil Red O staining.
Result:1.Compared with the control group,The insulin sensitivity index of MIR group was lower significantly. Through aerobic exercise and / or to change normal diet,insulin sensitivity index was increased significantly.High fat diet can induce insulin resistance in the rats.
2.The fat cells of liver is dyed a deep red by Oil Red O staining. The fat cells of the liver of the rats of the control group spread out evenly, however, the fat cells of the liver of the rats of the high fat diet group are significantly increased, with masses to red lipid drops.After aerobic exercise and/or dietary intervention,Compared with the high fat diet group ,the fat cells of the liver of the three intervention groups decreased significantly .Compared with the control group,the fat cells of the liver of the ordinary diet group and the ordinary diet exercise group are close to the normal ones.Compared with the high fat diet group,the high fat diet exercise group decreased significantly,too.
3.The changes in the PPAR-γof cardiac muscle after intervention:Compared with the control group,the expression of the PPAR-γof the high fat diet exercise group and the ordinary diet exercise group increased significantly(P<0.05),but the expression of the PPAR-γof the high fat diet group decreased significantly(P<0.05);Compared with the high fat diet group ,the expression of the PPAR-γof the high fat diet exercise group and the ordinary diet exercise group increased significantly(P<0.05),although the expression of the PPAR-γof the ordinary diet group increased,however,there is no statistical significance.
Conclusions :1.After high fat diet of 8 weeks to SD rats, we can establish animal models of insulin resistance. 2.Aerobic exercise and the change to regular diet both can improve the condition of IR significantly.
研究方法:选用清洁级标准健康雄性SD大鼠215只,7周龄,206-257g,适应性训练一周后,分为A.安静对照组(CON)15只,B.胰岛素抵抗模型组200只,测量空腹血糖,放射免疫法测血胰岛素,计算胰岛素敏感指数,确定胰岛素抵抗大鼠模型的建立。建模成功后分为四组:高脂膳食组(DIO)、高脂膳食运动组(DE)、标准饲料组(NOR)、标准饲料运动组(NE)。八周干预后,各运动组大鼠于末次运动训练后和各安静组,于第二天早晨水合氯醛腹腔麻醉,取心脏和肝脏,置于-80°C冰箱保存。western blot半定量测定PPAR-γ表达;HE染色定性分析肝组织和心肌细胞的形态学变化,油红O染色半定量分析肝组织脂质的变化和定性分析心肌脂质的变化。
研究结果:1.胰岛素抵抗组大鼠胰岛素敏感指数明显低于对照组。通过有氧运动或/和标准饲料,胰岛素抵抗大鼠胰岛素敏感指数显著升高。
2.肝组织的脂质出现油红O明显着色:深红色。安静对照组大鼠的肝组织着色均匀地分散开来,而高脂组大鼠的肝组织着色明显,并且着色加深,着色面积明显增大;进行有氧运动和/或标准饲料后,干预组大鼠的肝组织与高脂组大鼠肝组织相比,着色面积明显减少;其中标准饲料组大鼠和标准饲料运动组大鼠的肝组织着色状况,与安静对照组大鼠的肝组织相比,已趋于正常;高脂膳食运动组大鼠的肝组织着色面积与高脂膳食组大鼠相比,显著性减少。
3.大鼠心肌的PPAR-γ经干预后的变化:与安静对照组(CON)相比,高脂膳食+运动组(DE)和标准饲料+运动组(NE)大鼠心肌的PPAR-γ的表达量明显增高(P<0.05),而高脂膳食组(DIO)大鼠心肌的PPAR-γ的表达量明显降低(P<0.05);与高脂膳食组(DIO)大鼠相比,干预三组的PPAR-γ均有所增高,其中高脂膳食+运动组(DE)和标准饲料+运动组(NE)大鼠心肌的PPAR-γ的表达量明显上升(P<0.05),而标准饲料组(NOR)虽有所增高,但无统计学意义(P>0.05)。
研究结论:1.连续8周的高脂高糖膳食能建立胰岛素抵抗大鼠模型。2.有氧运动或/和标准饲料均可改善胰岛素抵抗中脂质代谢紊乱,改善胰岛素抵抗状态。
Obejective: To observe the effect of aerobic exercise and standard fodder which has affected the lipid metabolism of the insulin resistance rats which was fed by the fatty and high sugar .
Methods:To select 215 clean and healthy male Sprague-Dawley(SD)(purchased from B&K Universal Group Limited )which were 7 weeks age and weighted from 206g to 257g,after adaptive training for a week,the paper divided them into 2 groups stochastically:A、Control,C,n=15,B、Model of Insulin Resistance,MIR,n=200.After buliding the model of IR rats, then divided into 4 groups of MIR,were high fat diet control group(DIO),high fat diet exercise group(DE),ordinary diet group(NOR) and ordinary diet exercise group(NE). Exercise 8 weeks.Measure the blood glucose immediately and the insulin by radioimmunoassay,calculate insulin sensitivity index to determine the model of the insulin resistance in the rat established.
After the high fat diet exercise group and ordinary diet exercise group finished its last exercises training,in the next morning the each groups rats were given a general anesthesia with chloralhydrate injection into the abdominal cavity,then were killed. Take the heart of the rat to the fridge of -80°c . Analyze the PPAR-γin cardiac muscle by western blot quantificationally, analyze the morphological changes of the hepar and myocardial cells qualitatively,analyze the adipose change of the hepar and myocardial cells by Oil Red O staining.
Result:1.Compared with the control group,The insulin sensitivity index of MIR group was lower significantly. Through aerobic exercise and / or to change normal diet,insulin sensitivity index was increased significantly.High fat diet can induce insulin resistance in the rats.
2.The fat cells of liver is dyed a deep red by Oil Red O staining. The fat cells of the liver of the rats of the control group spread out evenly, however, the fat cells of the liver of the rats of the high fat diet group are significantly increased, with masses to red lipid drops.After aerobic exercise and/or dietary intervention,Compared with the high fat diet group ,the fat cells of the liver of the three intervention groups decreased significantly .Compared with the control group,the fat cells of the liver of the ordinary diet group and the ordinary diet exercise group are close to the normal ones.Compared with the high fat diet group,the high fat diet exercise group decreased significantly,too.
3.The changes in the PPAR-γof cardiac muscle after intervention:Compared with the control group,the expression of the PPAR-γof the high fat diet exercise group and the ordinary diet exercise group increased significantly(P<0.05),but the expression of the PPAR-γof the high fat diet group decreased significantly(P<0.05);Compared with the high fat diet group ,the expression of the PPAR-γof the high fat diet exercise group and the ordinary diet exercise group increased significantly(P<0.05),although the expression of the PPAR-γof the ordinary diet group increased,however,there is no statistical significance.
Conclusions :1.After high fat diet of 8 weeks to SD rats, we can establish animal models of insulin resistance. 2.Aerobic exercise and the change to regular diet both can improve the condition of IR significantly.
引文
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[2]Lauterio TJ, Bond JP, Ulman EA. Development and characrization of a purified diet to identify obesity-susceptible and resistant rat populations[J]. J Nutr, 1994,124(11):2127-2178.
[3]李光伟,潘长玉,李晖.口服葡萄糖耐量试验葡萄糖、胰岛素曲线下面积比作为胰岛素敏感性指数的缺陷[J].中华内科杂志,1997,36(3):153-155.
[4]Bedfod TG,Tipton CM,Wilson NC,et al. Maximum oxygen consumption of rats and its changes with various experimental procedures[J]. J Appl Physio,1979,47(6):1278-1283.
[5]李秀平,孙静,张俊权等.慢性应激对大鼠葡萄糖耐量及胰岛素抵抗的影响[J].中国医药导报,2007,4(28):25-26.
[6]许岭翎,向红丁,张荣等.高脂饮食诱发血糖升高的动物模型中瘦素与胰岛素变化的关系[J].基础医学与临床,2001,21(5):441-444.
[7]Holloszy JO.Exercise-induced increase in muscle insulin sensitivity[J].J Appl Physiol,2005,99(1):338-343.
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