针刺家兔耳穴对旁巨细胞外侧核活动改变的影响及机制探讨
|
摘要
目的 观察耳针对家兔旁巨细胞外侧核(PGL)活动改变的影响，尝试从递质水平上探讨耳针机制。方法 将60只健康家兔随机分为：1．胸肺区组(PC组)：(1)生理盐水组(N组)；(2)荷包牡丹碱组(B组)；2．非胸肺区组(nPC组)：(1)生理盐水组(N’组)；(2)荷包牡丹碱组(B’组)。记录不同时点的平均动脉压(MAP)、脉压、心率、呼吸压、呼吸频率，综合分析，研究耳针的调整效应及作用机制。结果(一)PC组1．N组：PGL单独微量注射生理盐水(NS)，对血压和呼吸无明显影响；注射NS并同时电针胸肺区，与单独注射NS相比，血压和呼吸无明显改变。2．B组：PGL单独微量注射荷包牡丹碱(BIC)，MAP升高，而脉压、心率、呼吸压、呼吸频率无明显改变；PGL注射BIC并同时电针胸肺区，与单独注射BIC相比，MAP升高幅度得到明显调整；对MAP恢复正常的时间、脉压、心率、呼吸压及呼吸频率无明显影响。(二)nPC组1．N’组：PGL单独微量注射NS，对血压和呼吸无明显影响；注射NS并同时电针非胸肺区，与单独注射NS相比，血压和呼吸无明显改变。2．B’组：PGL单独微量注射BIC，MAP升高，而脉压、心率、呼吸压、呼吸频率无明显改变；PGL注射BIC并同时电针非胸肺区，与单独注射BIC相比，MAP升高幅度得到明显调整；对MAP恢复正常的时间、脉压、心率、呼吸压及呼吸频率无明显影响。结论 1．电针耳郭胸肺区或非胸肺区均不干扰正常生理指标，对偏离正常状态的生理指标则有良性调整作用；2．电针耳穴胸肺区、非胸肺区对心血
管功能产生调节的机理可能与PGL的GABA递质系统有关;3.电针耳穴
的调节机制在不同状况下可能受某些因素的不同调控;4，耳郭胸肺区与非
胸肺区是有区别的耳区，但功能的特异性是相对的。5.如果PGL参与呼吸
调控，递质GABA可能不参与介导这一调控功能。
Objective To investigate the mechanism of ear acupuncture by studying the adjustment of ear acupuncture on activity of the nucleus paragigantocellularis lateralis of rabbit. Methods 60 health rabbits were divided randomly into: 1. pneumocardial area group(PC group): (1) normal saline group (N group); (2) bicuculline group (B group); 2.non-pneumocardial area group (nPC group): (1) normal saline group (N' group); (2) bicuculline group (B' group). Mean arterial pressure (MAP), pulse pressure, heart rate(HR), respiratory pressure and frequency of respiration at different moment were recorded for analysis. Results _I PC group: 1.N group: When only NS was microinjected into PGL, blood pressure and respiration did not change significantly; When NS was applied into PGL with ear acupuncture in PC simultaneously, compare with the former, blood pressure and respiration did not change significantly. 2. B group: When only bicuculline was microinjected into PGL, MAP was significantly higher; Pulse pressure, HR, respiratory pressure and frequency of respiration did not change significantly. When BIC was applied into PGL with ear acupuncture in PC simultaneously, compare with the former, MAP was not so much high; The time of MAP came back to normal level, pulse pressure, HR, respiratory pressure and frequency of respiration did not change significantly. II nPC group: l.N' group: When only NS was microinjected into PGL, blood pressure and respiration did not change significantly; When NS was applied into PGL with ear acupuncture in PC simultaneously, compare with the former, blood pressure and respiration did not change significantly. 2.B' group: When only bicuculline was
microinjected into PGL, MAP was significantly higher; Pulse pressure, HR, respiratory pressure and frequency of respiration did not change significantly. When BIC was applied into PGL with ear acupuncture in nPC simultaneously, compare with the former, MAP was not so much high; The time of MAP came back to normal level, pulse pressure, HR, respiratory pressure and frequency of respiration did not change significantly. Conclusion 1. Normal situation of body won't be disturbed by electronic acupuncturing either PC or nPC, but abnormal activities can be adjusted by it. 2. Possibly, mechanism of electronic ear acupuncture act on GABA system of PGL. 3. It seemed that the adjustment of ear acupuncture is controlled by some factors that are unknown now; 4. PC and nPC are different areas on ear. But the difference of adjustment between them is relative.5. In addition, if PGL is involved in adjustment to respiration, GABA maybe is not a role of it.
管功能产生调节的机理可能与PGL的GABA递质系统有关;3.电针耳穴
的调节机制在不同状况下可能受某些因素的不同调控;4，耳郭胸肺区与非
胸肺区是有区别的耳区，但功能的特异性是相对的。5.如果PGL参与呼吸
调控，递质GABA可能不参与介导这一调控功能。
Objective To investigate the mechanism of ear acupuncture by studying the adjustment of ear acupuncture on activity of the nucleus paragigantocellularis lateralis of rabbit. Methods 60 health rabbits were divided randomly into: 1. pneumocardial area group(PC group): (1) normal saline group (N group); (2) bicuculline group (B group); 2.non-pneumocardial area group (nPC group): (1) normal saline group (N' group); (2) bicuculline group (B' group). Mean arterial pressure (MAP), pulse pressure, heart rate(HR), respiratory pressure and frequency of respiration at different moment were recorded for analysis. Results _I PC group: 1.N group: When only NS was microinjected into PGL, blood pressure and respiration did not change significantly; When NS was applied into PGL with ear acupuncture in PC simultaneously, compare with the former, blood pressure and respiration did not change significantly. 2. B group: When only bicuculline was microinjected into PGL, MAP was significantly higher; Pulse pressure, HR, respiratory pressure and frequency of respiration did not change significantly. When BIC was applied into PGL with ear acupuncture in PC simultaneously, compare with the former, MAP was not so much high; The time of MAP came back to normal level, pulse pressure, HR, respiratory pressure and frequency of respiration did not change significantly. II nPC group: l.N' group: When only NS was microinjected into PGL, blood pressure and respiration did not change significantly; When NS was applied into PGL with ear acupuncture in PC simultaneously, compare with the former, blood pressure and respiration did not change significantly. 2.B' group: When only bicuculline was
microinjected into PGL, MAP was significantly higher; Pulse pressure, HR, respiratory pressure and frequency of respiration did not change significantly. When BIC was applied into PGL with ear acupuncture in nPC simultaneously, compare with the former, MAP was not so much high; The time of MAP came back to normal level, pulse pressure, HR, respiratory pressure and frequency of respiration did not change significantly. Conclusion 1. Normal situation of body won't be disturbed by electronic acupuncturing either PC or nPC, but abnormal activities can be adjusted by it. 2. Possibly, mechanism of electronic ear acupuncture act on GABA system of PGL. 3. It seemed that the adjustment of ear acupuncture is controlled by some factors that are unknown now; 4. PC and nPC are different areas on ear. But the difference of adjustment between them is relative.5. In addition, if PGL is involved in adjustment to respiration, GABA maybe is not a role of it.
引文
[1]、管遵信．耳穴诊治疾病的原理．中国针灸．1993，(1)：42
[2]、黄丽春．耳穴诊断治疗学．北京：科学技术文献出版社，2000．17～32
[3]、管遵信．中国耳针学．上海：上海科学技术出版社，1995．416～444
[4]、韩济生．神经科学原理．第二版．北京：北京医科大学出版社，1999．543～544
[5]、唐杰，魏书均，杨映宁等．耳体联系的实验研究—实验性胸膜炎家兔耳廓胸肺区的染色定位．云南中医杂志，1989，10(1)：40
[6]、姜淳，沉锷．网状巨细胞外侧核细胞发放特性的分析以及它们和腹侧群呼吸相关神经元的突触联系．见：陈子彬，刘磊主编．呼吸调节生理进展．第一版．上海：上海医科大学出版社，1989．141～148
[7]、李勤，宋刚．家兔延髓嘴端腹外侧区向膈神经核的轴突投射．山东医科大学学报，2000，38(2)：126
[8]、Barman S M, Gebber G L. Axonal projection pattern of ventrolateral medullospinal sympathoexcitatory neurons. Journal of Neurophysiology, 1985, (53): 1567
[9]、Brown D L, Guyenet P G. Electrophysiological study of cardiovascular neurons in the rostral ventrolateral medulla in rats. Circ. Res., 1985, (56): 359
[10]、Guyenet P G, Brown D L. Nucleus paragigantocellularis lateralis and lumbar sympathetic nerve discharge in the rat. American Journal of Physiology, 1988, (250): R1081
[11]、马秀英，范振中，何瑞荣．心室内和心外膜应用腺苷对延髓PGL神经元电活动的影响．生理学报，1997，(49)：504
[12]、Lovick T A. Tonic GABAergic and cholinergie influences on pain control and cardiovascular control neurones in nucleus paragigantoeellularis lateralis in the rat. Pain, 1987, (31): 401
[13]、Miao-Kun sun, Patrice G Guyenet. GABA-mediated baroreeeptor inhibition of reticulospinal neurons. American Journal of Physiology, 1985, (249): R672
[14]、Miao-Kun sun, Patrice G Guyenet. Arterial baroreeeptor and vagal inputs to sympathoexeitatory neurons in rat medulla. American-Journal of Physiology, 1987, (252): R699
[15]、Hideho Arita, Naoki Kogo, Kiyoshi Ichikawa.Locations of medullary neurons with non-phasic discharges excited by stimulation of central and/or peripheral chemoreceptors and by activation of nociceptors in cat.Brain Research, 1988, (442): 1
[16]、汪文，徐美丽．大鼠延髓旁巨细胞外侧核微量注射利多卡因对呼吸和血压的影响．中国应用生理杂志，1998，14(3)：217
[17]、汪文，郑煜，徐美丽．大鼠旁巨细胞外侧核微量注射利多卡因、L-谷氨酸钠的呼吸、血压效应．华西医大学报，1994，(25)：266
[18]、汤德安．实验针灸学入门．天津：天津科学技术出版社．1986．107
[19]、徐凤洲．电针耳尖穴对窒息家兔血压和呼吸及作用实验及临床研究．针刺研究，1992，(1)：36
[20]、赵钦，秦光明，曹淑英，等．测定一些化学元素的变化探讨耳穴染色机制．云南中医杂志，1988，9(5)：27
[2]、黄丽春．耳穴诊断治疗学．北京：科学技术文献出版社，2000．17～32
[3]、管遵信．中国耳针学．上海：上海科学技术出版社，1995．416～444
[4]、韩济生．神经科学原理．第二版．北京：北京医科大学出版社，1999．543～544
[5]、唐杰，魏书均，杨映宁等．耳体联系的实验研究—实验性胸膜炎家兔耳廓胸肺区的染色定位．云南中医杂志，1989，10(1)：40
[6]、姜淳，沉锷．网状巨细胞外侧核细胞发放特性的分析以及它们和腹侧群呼吸相关神经元的突触联系．见：陈子彬，刘磊主编．呼吸调节生理进展．第一版．上海：上海医科大学出版社，1989．141～148
[7]、李勤，宋刚．家兔延髓嘴端腹外侧区向膈神经核的轴突投射．山东医科大学学报，2000，38(2)：126
[8]、Barman S M, Gebber G L. Axonal projection pattern of ventrolateral medullospinal sympathoexcitatory neurons. Journal of Neurophysiology, 1985, (53): 1567
[9]、Brown D L, Guyenet P G. Electrophysiological study of cardiovascular neurons in the rostral ventrolateral medulla in rats. Circ. Res., 1985, (56): 359
[10]、Guyenet P G, Brown D L. Nucleus paragigantocellularis lateralis and lumbar sympathetic nerve discharge in the rat. American Journal of Physiology, 1988, (250): R1081
[11]、马秀英，范振中，何瑞荣．心室内和心外膜应用腺苷对延髓PGL神经元电活动的影响．生理学报，1997，(49)：504
[12]、Lovick T A. Tonic GABAergic and cholinergie influences on pain control and cardiovascular control neurones in nucleus paragigantoeellularis lateralis in the rat. Pain, 1987, (31): 401
[13]、Miao-Kun sun, Patrice G Guyenet. GABA-mediated baroreeeptor inhibition of reticulospinal neurons. American Journal of Physiology, 1985, (249): R672
[14]、Miao-Kun sun, Patrice G Guyenet. Arterial baroreeeptor and vagal inputs to sympathoexeitatory neurons in rat medulla. American-Journal of Physiology, 1987, (252): R699
[15]、Hideho Arita, Naoki Kogo, Kiyoshi Ichikawa.Locations of medullary neurons with non-phasic discharges excited by stimulation of central and/or peripheral chemoreceptors and by activation of nociceptors in cat.Brain Research, 1988, (442): 1
[16]、汪文，徐美丽．大鼠延髓旁巨细胞外侧核微量注射利多卡因对呼吸和血压的影响．中国应用生理杂志，1998，14(3)：217
[17]、汪文，郑煜，徐美丽．大鼠旁巨细胞外侧核微量注射利多卡因、L-谷氨酸钠的呼吸、血压效应．华西医大学报，1994，(25)：266
[18]、汤德安．实验针灸学入门．天津：天津科学技术出版社．1986．107
[19]、徐凤洲．电针耳尖穴对窒息家兔血压和呼吸及作用实验及临床研究．针刺研究，1992，(1)：36
[20]、赵钦，秦光明，曹淑英，等．测定一些化学元素的变化探讨耳穴染色机制．云南中医杂志，1988，9(5)：27