针刺家兔耳郭“胸肺区”对旁巨细胞外侧核心血管效应的影响
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摘要
目的 探讨耳针对家兔旁巨细胞外侧核(PGL)心血管效应的影响及其可能机制。方法 本实验以40只家兔为实验动物，以平均动脉压(MAP)、心率(HR)、左心室内压峰值(LVSP)、左心室内压变化上升最大速率(LV+dp／dt max)及肾交感神经放电(RSND)为指标，于PGL微量注射药物，改变神经元的活动，观察其对心血管功能的影响，然后针刺耳郭“胸肺区”(TPA)，观察其对药物所致的心血管功能改变的影响。结果 (1)PGL微量注射生理盐水(NS)，各指标无明显变化：注射NS的同时针刺TPA或nTPA，RSND增加，其余各指标无明显变化。(2)PGL微量注射谷氨酸(Glu)，MAP、LVSP、LV+dp／dt max升高，RSND增加，HR无明显变化；注射Glu的同时针刺TPA时，MAP、LVSP及RSND的变化幅度减小，而对LV+dp／dt max的变化无明显影响。(3)PGL微量注射谷氨酸(Glu)，MAP、LVSP、LV+dp／dt max升高，RSND增加，HR无明显变化；注射Glu的同时针刺nTPA时，对各指标的变化幅度均无明显影响。结论 (1)针刺家兔耳郭TPA对Glu兴奋PGL神经元引起的心血管功能偏离正常改变有调整作用，这种调整作用可能是通过抑制交感缩血管中枢来实现的。(2)耳郭TPA和nTPA表现出了耳针调整脏腑功能的穴位特异性作用。
Objective To investigate the mechanism of ear acupuncture by studying the effects of acupuncture of "thoracopneumatic area" (TPA) on the cardiovascular activity of nucleus paragigantocellularis lateralis(PGL) in rabbits . Methods The experiments were performed on 40 rabbits anesthetized with Urethan. The mean arterial pressure(MAP)x left intraventricular pressure(LVSP) , maximum rate of intraventricular pressure change(LV+dp/dt max), heart rate(HR) and renal sympathetic nerve discharge (RSND) were recorded. When microinjection into PGL induced the changes of cardiovascular activity, the effects of acupuncture of TPA on these changes were observed. Results (1) When NS was microinjected into PGL, MAP, HR, LVSP, LV+dp/dt max, RSND did not change significantly. When NS was microinjected into PGL with acupuncture in TPA or nTPA simultaneously, RSND increased, MAP, HR, LVSP, LV+dp/dt max did not change significantly. (2) The microinjection of Glutamate(Glu) into PGL brought about the increase of
MAP, LVSP, LV+dp/dt max and RSND. While the microinjection of Glu into PGL with acupuncture in TPA simultaneously, the extent of the variabilities of MAP, LVSP, RSND were reduced and the extent of the variabilities of LV+dp/dt max was not changed. (3) The microinjection of Glutamate(Glu) into PGL brought about the increase of MAP, LVSP, LV+dp/dt max and RSND. While the microinjection of Glu into PGL with acupuncture in nTPA simultaneously, all of the variabilities of MAP, LVSP, LV+dp/dt max and RSND were not changed significantly. Conclusion (1) Acupuncture of "thoracopneumatic area"on anesthetized rabbits can regulate the cardiovascular activities that were produced by the excitation of Glu through the microinjection of Glu into to PGL, and sympathetic vasoconstrictor center may be
2
responsible for this regluting action. (2) There was specialty of regluting action between "thoracopneumatic area" and "non-thoracopneumatic area".
Objective To investigate the mechanism of ear acupuncture by studying the effects of acupuncture of "thoracopneumatic area" (TPA) on the cardiovascular activity of nucleus paragigantocellularis lateralis(PGL) in rabbits . Methods The experiments were performed on 40 rabbits anesthetized with Urethan. The mean arterial pressure(MAP)x left intraventricular pressure(LVSP) , maximum rate of intraventricular pressure change(LV+dp/dt max), heart rate(HR) and renal sympathetic nerve discharge (RSND) were recorded. When microinjection into PGL induced the changes of cardiovascular activity, the effects of acupuncture of TPA on these changes were observed. Results (1) When NS was microinjected into PGL, MAP, HR, LVSP, LV+dp/dt max, RSND did not change significantly. When NS was microinjected into PGL with acupuncture in TPA or nTPA simultaneously, RSND increased, MAP, HR, LVSP, LV+dp/dt max did not change significantly. (2) The microinjection of Glutamate(Glu) into PGL brought about the increase of
MAP, LVSP, LV+dp/dt max and RSND. While the microinjection of Glu into PGL with acupuncture in TPA simultaneously, the extent of the variabilities of MAP, LVSP, RSND were reduced and the extent of the variabilities of LV+dp/dt max was not changed. (3) The microinjection of Glutamate(Glu) into PGL brought about the increase of MAP, LVSP, LV+dp/dt max and RSND. While the microinjection of Glu into PGL with acupuncture in nTPA simultaneously, all of the variabilities of MAP, LVSP, LV+dp/dt max and RSND were not changed significantly. Conclusion (1) Acupuncture of "thoracopneumatic area"on anesthetized rabbits can regulate the cardiovascular activities that were produced by the excitation of Glu through the microinjection of Glu into to PGL, and sympathetic vasoconstrictor center may be
2
responsible for this regluting action. (2) There was specialty of regluting action between "thoracopneumatic area" and "non-thoracopneumatic area".
引文
1．管遵信．耳穴诊治疾病的原理．中国针灸，1993，(1)：42
2．唐杰，魏书均，杨映宁等．耳体联系的实验研究——实验性胸膜炎家兔耳廓胸肺区的染色定位．云南中医杂志，1989，10(1)：40
3．周文琪，周定邦，杨映宁等．耳体联系的实验研究之二——家兔左侧实验性胸膜炎、耳廓胸肺区的染色定位．云南中医中药杂志，1996，17(6)：39
4．魏书均，赵钦，唐杰等．急性胸膜炎家兔的耳廓染色与体内几种化学元素含量的变化．中西医结合杂志，1991，(增刊)：345
5．戴路明，魏书均，周定邦等．激光照射耳廓“胸肺区”对孤束核腹外侧区活动的影响．昆明医学院学报，2000，21(2)：44
6．谭太贵，周文琪，周定邦等．刺激家兔耳廓“胸肺区”对延髓压力敏感区活动的影响(摘要)．昆明医学院学报，2002，23(2)：89
7．王立．针刺家兔耳穴对旁巨细胞外侧核活动改变的影响及机制探讨．研究生论文．
8．刘红巾，徐美丽．L-谷氨酸及其拮抗剂对大鼠脑片旁巨细胞外侧核神经元自发放电的影响．华西医大学报，1994，(25)：9
9．姜淳，沈锷．网状巨细胞外侧核细胞发放特性的分析以及它们和腹侧群呼吸相关神经元的突轴联系．见：陈子彬，刘磊主编．呼吸调节生理进展．第一版．上海：上海医科大学出版社，1989，141
10．李勤，宋刚．家兔延髓嘴端腹外侧区向膈神经核的轴突投射．山东医科大学学报，2000，38(2)：126
11. Barman S M, Gebber G L. Axonal projection pattern of ventrolateral medullospinal sympathoexcitatory neurons. Journal of Neurophsiology, 1985, (53): 1567
12. Brown D L, Guyenet P G.. Electrophysiological study of cardiovascular neurons in the rostral ventrolateral medulla in rots. Circulation Research, 1985, (56): 359
13. Guyenet P G, Brown D L. Nucleus paragigantocellularis lateralis and lumbar sympathetic nerve discharge in the rat. American Journal of phsiology, 1988,
(250)：R1081
14．黄河清，梁书忠．耳针心穴对高血压病血压及左心功能的改善作用．中西医结合杂志，1991，11(11)：654
15．王梅康，岳彩贵．针刺配合耳穴贴压治疗高血压36例临床观察．甘肃中医，1996，9(6)：32
16．管遵信，耳穴染色与内脏相关性的研究——家兔实验性阑尾炎手术后5天内之耳穴染色观察．云南中医杂志，1984，5(6)：39
17．李育良，李裕强，孙韬等．家兔内脏病理模型耳廓反应区的定位研究．针灸临床杂志，1995，11(1)：28
18．于海荣，刘豫安，袁亚非等．家兔血流动力学生理参数的测定．承德医学院学报，2001，18(1)：18
19．王月兰，汪克明，周逸平．温针灸“心俞”穴对自发性高血压大鼠的交感神经系统功能的影响．针刺研究，1997，22(3)：243
20．林文注．实验针灸学．上海：上海中医学院出版社，1989，170
2．唐杰，魏书均，杨映宁等．耳体联系的实验研究——实验性胸膜炎家兔耳廓胸肺区的染色定位．云南中医杂志，1989，10(1)：40
3．周文琪，周定邦，杨映宁等．耳体联系的实验研究之二——家兔左侧实验性胸膜炎、耳廓胸肺区的染色定位．云南中医中药杂志，1996，17(6)：39
4．魏书均，赵钦，唐杰等．急性胸膜炎家兔的耳廓染色与体内几种化学元素含量的变化．中西医结合杂志，1991，(增刊)：345
5．戴路明，魏书均，周定邦等．激光照射耳廓“胸肺区”对孤束核腹外侧区活动的影响．昆明医学院学报，2000，21(2)：44
6．谭太贵，周文琪，周定邦等．刺激家兔耳廓“胸肺区”对延髓压力敏感区活动的影响(摘要)．昆明医学院学报，2002，23(2)：89
7．王立．针刺家兔耳穴对旁巨细胞外侧核活动改变的影响及机制探讨．研究生论文．
8．刘红巾，徐美丽．L-谷氨酸及其拮抗剂对大鼠脑片旁巨细胞外侧核神经元自发放电的影响．华西医大学报，1994，(25)：9
9．姜淳，沈锷．网状巨细胞外侧核细胞发放特性的分析以及它们和腹侧群呼吸相关神经元的突轴联系．见：陈子彬，刘磊主编．呼吸调节生理进展．第一版．上海：上海医科大学出版社，1989，141
10．李勤，宋刚．家兔延髓嘴端腹外侧区向膈神经核的轴突投射．山东医科大学学报，2000，38(2)：126
11. Barman S M, Gebber G L. Axonal projection pattern of ventrolateral medullospinal sympathoexcitatory neurons. Journal of Neurophsiology, 1985, (53): 1567
12. Brown D L, Guyenet P G.. Electrophysiological study of cardiovascular neurons in the rostral ventrolateral medulla in rots. Circulation Research, 1985, (56): 359
13. Guyenet P G, Brown D L. Nucleus paragigantocellularis lateralis and lumbar sympathetic nerve discharge in the rat. American Journal of phsiology, 1988,
(250)：R1081
14．黄河清，梁书忠．耳针心穴对高血压病血压及左心功能的改善作用．中西医结合杂志，1991，11(11)：654
15．王梅康，岳彩贵．针刺配合耳穴贴压治疗高血压36例临床观察．甘肃中医，1996，9(6)：32
16．管遵信，耳穴染色与内脏相关性的研究——家兔实验性阑尾炎手术后5天内之耳穴染色观察．云南中医杂志，1984，5(6)：39
17．李育良，李裕强，孙韬等．家兔内脏病理模型耳廓反应区的定位研究．针灸临床杂志，1995，11(1)：28
18．于海荣，刘豫安，袁亚非等．家兔血流动力学生理参数的测定．承德医学院学报，2001，18(1)：18
19．王月兰，汪克明，周逸平．温针灸“心俞”穴对自发性高血压大鼠的交感神经系统功能的影响．针刺研究，1997，22(3)：243
20．林文注．实验针灸学．上海：上海中医学院出版社，1989，170