日粮维生素E对绵羊睾丸繁殖机能影响的分子机制初探
|
摘要
本团队前期研究证明,日粮维生素E可以提高公羔繁殖器官的发育、抗氧化性能、精子发育和精液品质。据此,为进一步探索日粮维生素E对公羊繁殖性能的作用机理,本研究选择健康断奶后20-30天的滩羊公羔35只(16.20±1.65kg),随机分为5个处理组,每组7只。对照组(E0)饲喂基础日粮,不添加维生素E；试验组维生素E的添加剂量分别为20(E20)、100(E100)、200(E200)、2000(E2000) IU羊-1.天-1,试验期为120天。屠宰后取睾丸及各组织,从维生素E转运因子、睾丸差异基因、抗氧化酶基因和生殖激素合成相关基因的角度,研究日粮维生素E对绵羊睾丸繁殖机能影响的分子机制,为揭示维生素E调控繁殖性能提供理论依据。
试验一：日粮维生素E对滩羊各组织α-生育酚转移蛋白(α-TTP)基因表达水平的影响。免疫组织化学试验表明：滩羊的心脏、脾脏、肺脏、肾脏组织均检测到α-TTP的阳性信号,并且α-TTP定位在细胞质中；而睾丸、背最长肌、臀肌没有检测到阳性信号。各组织α-TTP基因表达量的结果表明：肾脏、背最长肌和臀肌中,维生素E添加量对α-TTP mRNA表达量没有显著影响(P>0.05)。E200组肺脏α-TTP mRNA表达量显著(P<0.05)高于E2000组；E200组脾脏α-TTP mRNA表达量显著(P<0.05)高于E100组；与此相反,在心脏中,E200组α-TTP mRNA表达量显著(P<0.05)低于E0组。维生素E添加量对睾丸α-TTP mRNA表达量没有显著影响(P>0.05),但随着维生素E添加水平的提高,α-TTP mRNA的表达水平有一定的下降趋势,而E200和E2000组显著提高了睾丸维生素E含量(P<0.05)。本试验表明,日粮维生素E水平对滩羊不同组织α-TTP mRNA的表达量有一定的影响,并且组织间存在差异,影响维生素E在不同组织发挥作用。而日粮维生素E对睾丸α-TTP mRNA表达量和维生素E含量的影响,表明α-TTP水平与维生素E在睾丸发挥功能相关,但是与繁殖性能的直接关联性有待于进一步研究。
试验二：构建滩羊睾丸cDNA文库的构建并鉴定质量。结果表明：目前成功得到滩羊睾丸cDNA文库,其初级库容约2.0x106cfu,初级文库甘油菌保存液滴度为5.1×104cfu/1.8mL,插入片段平均长度为1Kb,重组率为93.75%,最终得到文库质粒约3.7mg。本试验表明滩羊睾丸cDNA文库符合建库要求,可作为进一步克隆滩羊睾丸特异表达基因的可靠材料,为研究滩羊睾丸繁殖性能提供基础。
试验三：日粮不同维生素E水平下滩羊睾丸差异基因的筛选。结果表明：通过GO生物学功能进行分析,差异基因参与发育过程、免疫系统过程、生物粘连生物学过程、能量脂质代谢过程和氧化应激应答过程。KEGG通路分析结果显示,筛选出的维生素E差异基因涉及PPAR信号通路、MAPK信号通路、细胞粘附、脂质代谢、糖类代谢和氧化相关基因。综合分析表明,维生素E调控睾丸基因表达的作用机制可能包括：调节组织氧化水平；调节信号通路中受体表达或转录因子活性；影响代谢相关基因的表达。
试验四：日粮维生素E对睾丸抗氧化酶基因mRNA和蛋白表达的影响。荧光定量结果显示：与对照组相比,E200组显著(P<0.05)提高了睾丸谷胱甘肽过氧化物酶3(GPx3)和谷胱甘肽转移酶A1(GSTA1) mRNA表达量,E20组则显著(P<0.05)提高了GPx3的mRNA表达量。Western blot结果显示：日粮维生素E显著(P<0.05)提高了睾丸GPx3蛋白表达量；与对照组相比,E20组和E100组显著(P<0.05)提高了GSTA1蛋白表达量。本试验表明,日粮添加维生素E能不同程度地提高GPx3和GSTA1mRNA或蛋白表达量,荧光定量结果与基因芯片反映的结果一致。
试验五：日粮维生素E对睾丸生殖激素相关基因mRNA和蛋白表达的影响。荧光定量结果显示：随着维生素E添加水平的增高,睾丸GATA结合蛋白4(GATA-4)、类固醇急性调节蛋白(StAR)、细胞色素P450A1(CYP11A1) mRNA的表达水平有先升高后下降的趋势。与对照组相比,E200组显著(P<0.05)提高了GATA-4的mRNA表达量,E20组三种基因rnRNA表达量显著提高(P<0.05)；而E100组三种基因mRNA表达量均有显著(P<0.05)低于E20,但是与对照组差异不显著(P>0.05)。Western blot结果显示：维生素E有提高GATA-4蛋白表达量的趋势；与对照组相比,E20和E2000组均显著(P<0.05)提高了睾丸StAR蛋白表达量。本试验表明,维生素E对生殖激素相关基因mRNA和蛋白表达量有一定的提高作用。
结论：日粮维生素E对绵羊睾丸基因表达有一定的调节作用,维生素E可在一定程度上提高抗氧化酶基因和生殖激素合成基因mRNA和蛋白表达量,进而提高抗氧化酶活性,是维生素E提高绵羊睾丸繁殖性能的主要原因。
The previous research conducted in our laboratory showed that dietary supplementation of vitamin E improved the development of reproductive organs, antioxidant properties, sperm development and semen quality in male kids. Under these results, this study was carried out to investigate the mechanism of dietary vitamin E promoting the ram reproduction performance. Thirty-five local male kids of Tan sheep (20to30days after weaning) with similar body weight (16.20±1.65kg) were randomly allocated into five groups (with7animals in each group) and fed with diets supplemented with0(control group),20,100,200and2,000IU sheep-1day-1vitamin E (treatments denoted as E0, E20, E100, E200, and E2000, respectively) for120days. Then the sheep were slaughtered and the samples of testis, heart, spleen, lung, kidney, longissimus dorsi and gluteus muscle were collected. In the present study, experiments those from the perspective of a-tocopherol transfer protein, differential genes, antioxidant enzyme, and reproductive hormone synthesis related genes, were conducted to investigate the molecular mechanism of promoting the testis reproduction performance by dietary vitamin E in Tan sheep.
Exp.1:Dietary vitamin E affects a-TTP mRNA levels in different tissues of the Tan sheep. The results showed that in heart, spleen, lung, and kidney tissues, a-TTP was located mainly in the cytoplasm, while no a-TTP immunoreactivity was detected in the cytoplasm of both the testis, longissimus dorsi and gluteus muscle samples. In the present study, the results showed that dietary vitamin E did not affect the a-TTP mRNA levels in the kidney, the longissimus dorsi muscle and the gluteus muscle. However, compared with the E100group, the E200group had higher (P<0.05) α-TTP mRNA levels in spleen. Similarly, a-TTP mRNA levels in the lung in E200group were higher (P<0.05) than those in the group of E2000. In contrast, compared with the control, the E200group had lower (P<0.05) a-TTP mRNA levels in heart tissues. Dietary vitamin E did not affect the testis a-TTP mRNA levels, but there has a trend of decrease in response to increased dosage. While compared with the control, the E200and E2000group had significantly (P<0.05) higher vitamin E concentration in testis. In conclusion, dietary vitamin E levels had effects on the expression levels of a-TTP in some tissues of Tan sheep and the effect was clearly tissue-specific. And the relevance between the a-TTP mRNA levels and vitamin E concentration in testis warrant further investigation.
Exp.2:cDNA library of testis from Tan sheep was constructed with plasmid vector and the quality of the cDNA libraries was identified. The results showed that the titer of testis cDNA library was5.1×104cfu/1.8mL, the total number of colony-forming units (cfu) was2.0×106cfu, the recombinant rate was93.75%and the average insert size was about1.0Kb. The library titer, recombinant rate and the average insert size all matched quality requirements for cDNA library and this indicated that cDNA libraries of ovine testis was successfully constructed, which will be valuable resource for reproduction performance of the Tan sheep.
Exp.3:Tan sheep testis samples from different dietary vitamin E were collected to research the differential expression genes in our experiment by gene chip. The differential expression genes by GO analysis can find notations on development, response to reactive oxygen species, DNA metabolic process, cell adhesion, and lipid transport. The differential expression genes by KEGG pathway analysis had founded in the pathway of PPAR、 MAPK, Toll-like receptor signaling pathway, focal adhesion, glutathione metabolism and starch and sucrose metabolism. Based on the analysis of these genes, it was speculated that vitamin E might affect gene expression of testis by modulating the oxidation level, by affecting the expression of receptor and transcription factors in pathway, and regulating the expression of metabolism-associated genes.
Exp.4:Dietary vitamin E affects the expression of antioxidant enzyme genes in testis of the Tan sheep. The results showed that the E200group had higher (P<0.05) GPx3and GSTA1mRNA levels and E20group had higher (P<0.05) GPx3mRNA levels compared with the control. Dietary vitamin E could increase (P<0.05) the GPx3protein expression of testis. While compared with the control, E20and E100group had higher (P<0.05) GSTA1protein levels. In conclusion, dietary vitamin E could increase (P<0.05) the mRNA and protein expression of GPx3and GSTA1at different levels. The results of qRT-PCR for antioxidant enzyme genes were consistent with those from gene chip.
Exp.5:Dietary vitamin E affects the expression of reproductive hormone synthesis related genes in testis of the Tan sheep. The results showed that:As the levels of vitamin E increasing from0to2000IU sheep-1day-1diet, mRNA expression of GATA-4, StAR and CYP11A1first increased, then decreased. Compared with the control, the E20group increased (P<0.05) these three genes mRNA levels and the E200group increased (P<0.05) the expression of GATA-4mRNA. In contrast, these three genes mRNA levels in E100group decreased (P<0.05) compared with the E20group. And the E20and E2000group had the highest (P<0.05) protein expression of StAR. In conclusion, dietary vitamin E could increase (P<0.05) the mRNA and protein expression of GATA-4, StAR and CYP11A1at different levels.
It was concluded that the supplementation of vitamin E could regulate the gene expression in testis. Dietary vitamin E could increase the mRNA and protein expression of reproductive hormone synthesis related genes and antioxidant enzyme genes, and then increase the activity of antioxidant enzyme, were the main reasons for promoting the testis reproduction performance by dietary vitamin E.
试验一：日粮维生素E对滩羊各组织α-生育酚转移蛋白(α-TTP)基因表达水平的影响。免疫组织化学试验表明：滩羊的心脏、脾脏、肺脏、肾脏组织均检测到α-TTP的阳性信号,并且α-TTP定位在细胞质中；而睾丸、背最长肌、臀肌没有检测到阳性信号。各组织α-TTP基因表达量的结果表明：肾脏、背最长肌和臀肌中,维生素E添加量对α-TTP mRNA表达量没有显著影响(P>0.05)。E200组肺脏α-TTP mRNA表达量显著(P<0.05)高于E2000组；E200组脾脏α-TTP mRNA表达量显著(P<0.05)高于E100组；与此相反,在心脏中,E200组α-TTP mRNA表达量显著(P<0.05)低于E0组。维生素E添加量对睾丸α-TTP mRNA表达量没有显著影响(P>0.05),但随着维生素E添加水平的提高,α-TTP mRNA的表达水平有一定的下降趋势,而E200和E2000组显著提高了睾丸维生素E含量(P<0.05)。本试验表明,日粮维生素E水平对滩羊不同组织α-TTP mRNA的表达量有一定的影响,并且组织间存在差异,影响维生素E在不同组织发挥作用。而日粮维生素E对睾丸α-TTP mRNA表达量和维生素E含量的影响,表明α-TTP水平与维生素E在睾丸发挥功能相关,但是与繁殖性能的直接关联性有待于进一步研究。
试验二：构建滩羊睾丸cDNA文库的构建并鉴定质量。结果表明：目前成功得到滩羊睾丸cDNA文库,其初级库容约2.0x106cfu,初级文库甘油菌保存液滴度为5.1×104cfu/1.8mL,插入片段平均长度为1Kb,重组率为93.75%,最终得到文库质粒约3.7mg。本试验表明滩羊睾丸cDNA文库符合建库要求,可作为进一步克隆滩羊睾丸特异表达基因的可靠材料,为研究滩羊睾丸繁殖性能提供基础。
试验三：日粮不同维生素E水平下滩羊睾丸差异基因的筛选。结果表明：通过GO生物学功能进行分析,差异基因参与发育过程、免疫系统过程、生物粘连生物学过程、能量脂质代谢过程和氧化应激应答过程。KEGG通路分析结果显示,筛选出的维生素E差异基因涉及PPAR信号通路、MAPK信号通路、细胞粘附、脂质代谢、糖类代谢和氧化相关基因。综合分析表明,维生素E调控睾丸基因表达的作用机制可能包括：调节组织氧化水平；调节信号通路中受体表达或转录因子活性；影响代谢相关基因的表达。
试验四：日粮维生素E对睾丸抗氧化酶基因mRNA和蛋白表达的影响。荧光定量结果显示：与对照组相比,E200组显著(P<0.05)提高了睾丸谷胱甘肽过氧化物酶3(GPx3)和谷胱甘肽转移酶A1(GSTA1) mRNA表达量,E20组则显著(P<0.05)提高了GPx3的mRNA表达量。Western blot结果显示：日粮维生素E显著(P<0.05)提高了睾丸GPx3蛋白表达量；与对照组相比,E20组和E100组显著(P<0.05)提高了GSTA1蛋白表达量。本试验表明,日粮添加维生素E能不同程度地提高GPx3和GSTA1mRNA或蛋白表达量,荧光定量结果与基因芯片反映的结果一致。
试验五：日粮维生素E对睾丸生殖激素相关基因mRNA和蛋白表达的影响。荧光定量结果显示：随着维生素E添加水平的增高,睾丸GATA结合蛋白4(GATA-4)、类固醇急性调节蛋白(StAR)、细胞色素P450A1(CYP11A1) mRNA的表达水平有先升高后下降的趋势。与对照组相比,E200组显著(P<0.05)提高了GATA-4的mRNA表达量,E20组三种基因rnRNA表达量显著提高(P<0.05)；而E100组三种基因mRNA表达量均有显著(P<0.05)低于E20,但是与对照组差异不显著(P>0.05)。Western blot结果显示：维生素E有提高GATA-4蛋白表达量的趋势；与对照组相比,E20和E2000组均显著(P<0.05)提高了睾丸StAR蛋白表达量。本试验表明,维生素E对生殖激素相关基因mRNA和蛋白表达量有一定的提高作用。
结论：日粮维生素E对绵羊睾丸基因表达有一定的调节作用,维生素E可在一定程度上提高抗氧化酶基因和生殖激素合成基因mRNA和蛋白表达量,进而提高抗氧化酶活性,是维生素E提高绵羊睾丸繁殖性能的主要原因。
The previous research conducted in our laboratory showed that dietary supplementation of vitamin E improved the development of reproductive organs, antioxidant properties, sperm development and semen quality in male kids. Under these results, this study was carried out to investigate the mechanism of dietary vitamin E promoting the ram reproduction performance. Thirty-five local male kids of Tan sheep (20to30days after weaning) with similar body weight (16.20±1.65kg) were randomly allocated into five groups (with7animals in each group) and fed with diets supplemented with0(control group),20,100,200and2,000IU sheep-1day-1vitamin E (treatments denoted as E0, E20, E100, E200, and E2000, respectively) for120days. Then the sheep were slaughtered and the samples of testis, heart, spleen, lung, kidney, longissimus dorsi and gluteus muscle were collected. In the present study, experiments those from the perspective of a-tocopherol transfer protein, differential genes, antioxidant enzyme, and reproductive hormone synthesis related genes, were conducted to investigate the molecular mechanism of promoting the testis reproduction performance by dietary vitamin E in Tan sheep.
Exp.1:Dietary vitamin E affects a-TTP mRNA levels in different tissues of the Tan sheep. The results showed that in heart, spleen, lung, and kidney tissues, a-TTP was located mainly in the cytoplasm, while no a-TTP immunoreactivity was detected in the cytoplasm of both the testis, longissimus dorsi and gluteus muscle samples. In the present study, the results showed that dietary vitamin E did not affect the a-TTP mRNA levels in the kidney, the longissimus dorsi muscle and the gluteus muscle. However, compared with the E100group, the E200group had higher (P<0.05) α-TTP mRNA levels in spleen. Similarly, a-TTP mRNA levels in the lung in E200group were higher (P<0.05) than those in the group of E2000. In contrast, compared with the control, the E200group had lower (P<0.05) a-TTP mRNA levels in heart tissues. Dietary vitamin E did not affect the testis a-TTP mRNA levels, but there has a trend of decrease in response to increased dosage. While compared with the control, the E200and E2000group had significantly (P<0.05) higher vitamin E concentration in testis. In conclusion, dietary vitamin E levels had effects on the expression levels of a-TTP in some tissues of Tan sheep and the effect was clearly tissue-specific. And the relevance between the a-TTP mRNA levels and vitamin E concentration in testis warrant further investigation.
Exp.2:cDNA library of testis from Tan sheep was constructed with plasmid vector and the quality of the cDNA libraries was identified. The results showed that the titer of testis cDNA library was5.1×104cfu/1.8mL, the total number of colony-forming units (cfu) was2.0×106cfu, the recombinant rate was93.75%and the average insert size was about1.0Kb. The library titer, recombinant rate and the average insert size all matched quality requirements for cDNA library and this indicated that cDNA libraries of ovine testis was successfully constructed, which will be valuable resource for reproduction performance of the Tan sheep.
Exp.3:Tan sheep testis samples from different dietary vitamin E were collected to research the differential expression genes in our experiment by gene chip. The differential expression genes by GO analysis can find notations on development, response to reactive oxygen species, DNA metabolic process, cell adhesion, and lipid transport. The differential expression genes by KEGG pathway analysis had founded in the pathway of PPAR、 MAPK, Toll-like receptor signaling pathway, focal adhesion, glutathione metabolism and starch and sucrose metabolism. Based on the analysis of these genes, it was speculated that vitamin E might affect gene expression of testis by modulating the oxidation level, by affecting the expression of receptor and transcription factors in pathway, and regulating the expression of metabolism-associated genes.
Exp.4:Dietary vitamin E affects the expression of antioxidant enzyme genes in testis of the Tan sheep. The results showed that the E200group had higher (P<0.05) GPx3and GSTA1mRNA levels and E20group had higher (P<0.05) GPx3mRNA levels compared with the control. Dietary vitamin E could increase (P<0.05) the GPx3protein expression of testis. While compared with the control, E20and E100group had higher (P<0.05) GSTA1protein levels. In conclusion, dietary vitamin E could increase (P<0.05) the mRNA and protein expression of GPx3and GSTA1at different levels. The results of qRT-PCR for antioxidant enzyme genes were consistent with those from gene chip.
Exp.5:Dietary vitamin E affects the expression of reproductive hormone synthesis related genes in testis of the Tan sheep. The results showed that:As the levels of vitamin E increasing from0to2000IU sheep-1day-1diet, mRNA expression of GATA-4, StAR and CYP11A1first increased, then decreased. Compared with the control, the E20group increased (P<0.05) these three genes mRNA levels and the E200group increased (P<0.05) the expression of GATA-4mRNA. In contrast, these three genes mRNA levels in E100group decreased (P<0.05) compared with the E20group. And the E20and E2000group had the highest (P<0.05) protein expression of StAR. In conclusion, dietary vitamin E could increase (P<0.05) the mRNA and protein expression of GATA-4, StAR and CYP11A1at different levels.
It was concluded that the supplementation of vitamin E could regulate the gene expression in testis. Dietary vitamin E could increase the mRNA and protein expression of reproductive hormone synthesis related genes and antioxidant enzyme genes, and then increase the activity of antioxidant enzyme, were the main reasons for promoting the testis reproduction performance by dietary vitamin E.
引文
陈厚仰,汤育新,文甲明,等.SMART技术构建恒河猴睾丸组织全长cDNA文库.中国男科学杂志,2009,23(9)：13-16
邓效禹,宿甲子,杨焕民,等.籽鹅垂体组织全长cDNA文库的构建及部分克隆序列分析.中国兽医学报,2011,31(4)：544-547
董重阳,康波,贾晓剑,等.籽鹅卵巢组织全长cDNA文库的构建及部分克隆序列分析.农业生物技术学报,2010,18(2)：389-393
冯迎春,颜其贵,郭万柱,等.山羊地方性鼻内肿瘤病毒ENTV-SC株基因组cDNA文库的构建及生物信息学分析.中国兽医科学,2011,41(2)：126-130
郭俣,刘长青,陆涛峰,等.北京油鸡cDNA文库的构建及purH基因的表达.南京农业大学学报,2010,33(4)：93-99
何震,谢玉波,肖强.人正常胃黏膜细胞全长cDNA文库的构建及鉴定.成都医学院学报,2010,5(1)：8-11
胡兰,孙长勉,刘梅.维生素E对肉仔鸡抗热应激的影响.沈阳农业大学学报,2001,32(2)：117-119
胡文琴,孟庆利,王恬.维生素E对畜禽繁殖性能的影响.当代畜禽养殖业,2004,(5)：15-16
贾金龙.兴凯湖梅花鹿肝脏全长cDNA文库的构建及鉴定：[硕士学位论文].北京：中国农业科学院,2007
孔亚坤,耿秀琴,尹俊杰,等.维生素E对老年雌性大鼠雌激素及海马芳香化酶表达的影响.中国老年学杂志,2009,29：1058-1060
李靖,徐晓霞,韩本立,等.正常肝组织与肝癌组织差异表达基因消减cDNA文库的构建.中华肝脏病杂志,2001,9(6)：346-348
李苏.不同剂量维生素E对更年期大鼠卵巢功能的影响：[硕士学位论文].济南：山东大学,2008
李鑫,邹毅辉,杨晓亮,等.中国美利奴羊外周免疫器官cDNA文库的构建及鉴定.石河子大学学报,2010,28(5)：529-533
梁明振,卢克焕,刘芳.饲粮锌、硒和维生素E水平对经产母猪繁殖性能及相关参数的影响.中国兽医学报,2003,23(6)：620-623
刘洪波,罗海玲,刘志宏,等.日粮维生素E水平对山羊精液品质的影响.中国畜牧杂志,2005,41(10)：12-14
刘坡,李凯彬,王芳,等.剑尾鱼肝脏cDNA文库的构建.畜牧与兽医,2007,39(5)：12-15
刘学剑.维生素E的生理功能与动物生产.江西饲料,2000(3)：16-17
柳淑芳.小尾寒羊多胎性状相关基因的鉴定和遗传研究：[博士学位论文].泰安：山东农业大学,2004
卢圣栋.现代分子生物学实验技术(第二版).北京：中国协和医科大学,1999
陆涛峰.鄂伦春马cDNA文库构建及ccLZ基因的克隆与表达研究：[硕士学位论文].北京：中国农业科学院,2008
罗海玲,贾志海,朱士恩,等.稀释液中添加维生素E对绵羊冷冻精液品质的影响.中国畜牧杂 志,2004,(11)：7-9
罗海玲,孟慧,朱虹,等.维生素E改善羊肉品质的机理初探.饲料工业,2010(S2)：57-63
马金彪.条锈菌诱导的小麦叶片cDNA文库构建及表达序列标签分析：[硕士学位论文].陕西：西北农林科技大学,2006
蒲俊华,王强.维生素A和维生素E对母畜繁殖性能的影响.畜牧与饲料科学,2006,3：51-52
秦胜芳,谭卫东,陈又南,等.恒河猴肝脏cDNA文库的构建.四川大学学报(医学版),2007,38(3)：378-381
沈倍奋.分子文库.北京：科学出版社,2001
石新辉,边连全,王娜.肥育后期添加VE毗啶羧酸铬对肥育猪肉品品质的影响.畜牧兽医杂志,2005,24(6)：1-4
史清河.维生素E对肉品质的改善作用.中国饲料,1999,3：9-12
孙佳音,应锋,韩晓冬.睾丸间质细胞中睾酮合成酶及蛋白表达的调控因子.生殖与避孕,2009,29(1)：42-47
孙永风,李立勇,武明宇.维生素E营养研究进展.现代农业科技,2007,23：209-210
王彩玲,苗军.蛋鸡热应激的营养疗法.中国家禽,2002(6)：40-41
王传蓉.维生素E和硒对荷斯坦母牛及新生犊牛的影响：[硕士学位论文].江苏：扬州大学,2008
汪开毓,周震,耿毅.鲤维生素E缺乏的病理学研究.内蒙古农业大学学报,2005,27：165-166
王丽.围产期奶牛日粮添加不同水平维生素E对其生产性能、免疫功能及繁殖机能的影响：[硕士学位论文].内蒙古：内蒙古农业大学,2010
王秀利,李宁,赵志辉,等.香猪肌肉组织cDNA文库的构建及其EST测序成功率的分析.遗传,2002,24(3)：263-266
王秀利,李宁,赵兴波,等.丝毛乌骨鸡胸肌和肝脏cDNA文库的构建与鉴定.农业生物技术学报,2003,11(1)：60-63
王远志,陈创夫,盛金良,等.绵羊种布鲁氏菌019株侵染绵羊巨噬细胞cDNA文库的构建.中国人兽共患病学报,2008,24(4)：334-337
吴伟,徐日福,高光,等.鹅肌肉组织全长cDNA文库构建及泛素基因序列分析.畜牧与兽医,2007,39(5)：12-15
徐日福,吴伟,高光,等.鹅肝脏组织的质粒cDNA文库构建.吉林农业大学学报,2007,29(3)：325-328
闫顺,李付美,曲志才,等.莱芜猪肝脏组织全长cDNA文库的构建和鉴定.生物技术通报,2010,21(3)：397-400
杨波涛,陈凤香,莫文莲,等.我国食用植物油维生素E含量研究.粮油加工,2009(9)：52-55
杨畅,方福德.基因芯片数据分析.生命科学,2004,16(2)：41-48
杨丽萍,杨慧荣,张勇.中国大鲵脑cDNA文库构建及促甲状腺激素β亚基基因cDNA的克隆和序列分析.水产学报,2005,32(4)：507-516
岳度兵.维生素E对生长期敖汉细毛羊精液品质的影响及作用机理：[硕士学位论文].北京：中国农业大学,2009
张锦前,牛俊奇,王峰.人类肝脏cDNA文库的构建及hHGF基因的筛选、克隆与表达.生物医 学工程与临床,2006,10(5)：315-319
张亮,史雨红,陈炯,等.香鱼肝胰腺cDNA文库的构建和鉴定.水产科学,2011,30(1)：11-16
郑景洲,许友卿,丁兆坤.维生素E的功能、吸收与代谢(2).生物学通报,2005,40(12)：1-2
郑鹏然,周树南.食品卫生全书.北京：红旗出版社,1996
周显青,孙儒泳,牛翠娟.维生素E对免疫功能的影响.生理科学进展,2000(2)：163-165
朱虹.维生素E对波尔山羊公羔生殖器官抗氧化性能及其发育的影响：[硕士学位论文].北京：中国农业大学,2008
Abdala-Valencia H, Berdnikovs S, Cook-Mills J M. Vitamin E isoforms differentially regulate intercellular adhesion molecule-1 activation of PKCalpha in human microvascular endothelial cells. PLoS One,2012,7(7):e41054-e41058
Acharya U R, Mishra M, Mishra I, et al. Potential role of vitamins in chromium induced spermatogenesis in Swiss mice. Environmental Toxicology and Pharmacology,2004,15(2-3): 53-59
Acharya U R, Mishra M, Patro J, et al. Effect of vitamins C and E on spermatogenesis in mice exposed to cadmium. Reproductive Toxicology,2008,25(1):84-88
Adamstone F B. The effects of vitamin-E deficiency on the development of the chick. Journal of Morphology,2005,52(1):47-89
Anthony D Q. Evaluating the effects of maternal and progeny dietary supplementation of selenium yeast and vitamin E on the performance of broiler-breeder hens and performance and meat quality of progeny. The U S:University of Kentucky,2012
Adams M D, Kelley J M, Gocayne J D, et al. Complementary DNA sequencing:expressed sequence tags and human genome project. Science,1991,252(5013):1651-1656
AOAC. Official methods of analysis,5th ed. Washington:Association of Official Analytical Chemist, 1990
Arita M, Sato Y, Miyata A, et al. Human alpha-tocopherol transfer protein:cDNA cloning, expression and chromosomal localization. Biochemical Journal,1995,306 (Pt 2):437-443
Atkinson J, Epand R F, Epand R M. Tocopherols and tocotrienols in membranes:a critical review. Free Radical Biology and Medicine,2008,44(5):739-764
Aytan N, Jung T, Tamturk F, et al. Oxidative stress related changes in the brain of hypercholesterolemic rabbits. Biofactors,2008,33(3):225-236
Ban R, Takitani K, Kim H S, et al. alpha-Tocopherol transfer protein expression in rat liver exposed to hyperoxia. Free Radical Research,2002,36(9):933-938
Barella L, Muller P Y, Schlachter M, et al. Identification of hepatic molecular mechanisms of action of alpha-tocopherol using global gene expression profile analysis in rats. Biochimica Et Biophysica Acta,2004,1689(1):66-74
Bauernfeind J C, Rubin S H, Surmatis J D, et al. Carotenoids and fat-soluble vitamins:contribution to food, feed and Pharmaceuticals. Int Z Vitaminforsch,1970,40(3):391-416
Bella D L, Schock B C, Lim Y, et al. Regulation of the alpha-tocopherol transfer protein in mice:lack of response to dietary vitamin E or oxidative stress. Lipids,2006,41(2):105-112
Boscoboinik D, Szewczyk A, Azzi A. Alpha-tocopherol (vitamin E) regulates vascular smooth muscle cell proliferation and protein kinase C activity. Archives of Biochemistry and Biophysics,1991, 286(1):264-269
Boscoboinik D, Szewczyk A, Hensey C, et al. Inhibition of cell proliferation by alpha-tocopherol. Role of protein kinase C. Journal of Biological Chemistry,1991,266(10):6188-6194
Bozkurt M, Abuk M C, Alcicek A. Effect of dietary fat type on broiler breeder performance and
hatching egg characteristics. Journal of Applied Poultry Research,2008,17(1):47-53
Brigelius-Flohe R, Kluth D, and Banning A. Is there a future for antioxidants in atherogenesis? Molecular nutrition & food research,2005,49:1083-1089
Brigelius-Flohe R, Traber M G. Vitamin E:function and metabolism. Faseb Journal,1999,13(10): 1145-1155
Burton G W, Doba T, Gabe E J, et al. Autoxidation of biological molecules.4. Maximizing the antioxidant activity of phenols. Journal of the American Chemical Society,1985,107(24): 7053-7065
Burton G W, Traber M G, Acuff R V, et al. Human plasma and tissue a-tocopherol concentrations in response to supplementation with deuterated natural and synthetic vitamin E. American Journal of Clinical Nutrition,1998,67(4):669-684
Capper J L, Wilkinson R G, Kasapidou E, et al. The effect of dietary vitamin E and fatty acid
supplementation of pregnant and lactating ewes on placental and mammary transfer of vitamin E to
the lamb. British Journal of Nutrition,2005,93(4):549-557
Catalgol B, Kartal O N. Lipid rafts and redox regulation of cellular signaling in cholesterol induced atherosclerosis. Current Cardiology Reviews,2010,6(4):309-324
Cavalier L, Ouahchi K, Kayden H J, et al. Ataxia with isolated vitamin E deficiency:heterogeneity of mutations and phenotypic variability in a large number of families. American Journal of Human Genetics,1998,62(2):301-310
Chandra A K, Chatterjee A, Ghosh R, et al. Vitamin E-supplementation protect chromium (Ⅵ)-induced spermatogenic and steroidogenic disorders in testicular tissues of rats. Food and Chemical Toxicology,2010,48(3):972-979
Cherian G, Sim J S. Egg yolk polyunsaturated fatty acids and vitamin E content alters the tocopherol status of hatched chicks. Poultry Science,1997,76(12):1753-1759
Chiku S, Hamamura K, Nakamura T. Novel urinary metabolite of d-delta-tocopherol in rats. Journal of Lipid Research,1984,25(1):40-48
Cooperr D R, Kling O R, Carpenter M P. Effect of vitamin E deficiency on serum concentrations of follicle-stimulating hormone and testosterone during testicular maturation and degeneration.
Endocrinology,1987,120(1):83-90
Doria-Lamba L, De Grandis E, Cristiani E, et al. Efficacious vitamin E treatment in a child with ataxia with isolated vitamin E deficiency. European Journal of Pediatrics,2006,165(7):494-495
Du XJ, Wang J X, Liu N, et al. Identification and molecular characterization of a peritrophin-like protein from fleshy prawn (Fenneropenaeus chinensis). Molecular Immunology,2006,43(10): 1633-1644
Edery I, Chu L L, Sonenberg N, et al. An efficient strategy to isolate full-length cDNAs based on an mRNA cap retention procedure (CAPture). Molecular and Cellular Biology,1995,15(6): 3363-3371
Efimov V A, Chakhmakhcheva O G, Archdeacon J, et al. Detection of the 5'-cap structure of messenger RNAs with the use of the cap-jumping approach. Nucleic Acids Research,2001,29(22): 4751-4759
Evans H M, Bishop K. S. On the existence of a hitherto unrecognized dietary factor essential for reproduction. Science,1922,56(1458):650-651
Fechner H, Schlame M, Guthmann F, et al. alpha- and delta-tocopherol induce expression of hepatic alpha-tocopherol-transfer-protein mRNA. Biochemical Journal,1998,331 (Pt 2):577-581
Fernandez-Palacios H, Izquierdo M S, Gonzalez M, et al. Combined effect of dietary a-tocopherol and
n-3 HUFA on egg quality of gilthead seabream (Sparus aurata). Aquaculture,1998,161:475-476
Fischer A, Pallauf J, Gohil K, et al. Effect of selenium and vitamin E deficiency on differential gene expression in rat liver. Biochemical and Biophysical Research Communications,2001,285: 470-475
Fridovich I. Biological effects of the superoxide radical. Archives of Biochemistry and Biophysics, 1986,247(1):1-11
Fruebis J, Silvestre M, Shelton D, et al. Inhibition of VCAM-1 expression in the arterial wall is shared by structurally different antioxidants that reduce early atherosclerosis in NZW rabbits. Journal of Lipid Research,1999,40(11):1958-1966
Gabsi S, Gouider-Khouja N, Belal S, et al. Effect of vitamin E supplementation in patients with ataxia with vitamin E deficiency. European Journal of Neurology,2001,8(5):477-481
Gamal B M, Sherief M A, Hassan A H. Effect of combination of vitamin E and selenium injections on reproductive performance and blood parameters of Ossimi rams. Small Ruminant Research,2013, 113:103-108
Gohil K, Godzdanker R, O'Roark E, et al. Alpha-tocopherol transfer protein deficiency in mice causes multi-organ deregulation of gene networks and behavioral deficits with age. Annals of the New York Academy of Sciences,2004,1031:109-126
Gohil K, Oommen S, Quach H T, et al. Mice lacking alpha-tocopherol transfer protein gene have severe alpha-tocopherol deficiency in multiple regions of the central nervous system. Brain Research, 2008,1201:167-176
Grobas S, Mendez J, Lopez B C, et al. Effect of vitamin E and A supplementation on egg yolk alpha-tocopherol concentration. Poultry Science,2002,81(3):376-381
Hatfield P G, Robinson B L, Minikhiem D L, et al. Serum alpha-tocopherol and immune function in yearling ewes supplemented with zinc and vitamin E. Journal of Animal Science,2002,80(5): 1329-1334
Heemers H, Maes B, Foufelle F, et al. Androgens stimulate lipogenic gene expression in prostate cancer cells by activation of the sterol regulatory element-binding protein cleavage activating protein/sterol regulatory element-binding protein pathway. Molecular Endocrinology,2001,15(10): 1817-1828
Heemers H, Vanderhoydonc F, Roskams T, et al. Androgens stimulate coordinated lipogenic gene expression in normal target tissues in vivo. Molecular and Cellular Endocrinology,2003,205(1-2): 21-31
Hensley K, Benaksas E J, Bolli R, et al. New perspectives on vitamin E:c-tocopherol and carboxyelthylhydroxychroman metabolites in biology and medicine. Free Radical Biology and Medicine,2004,36(1):1-15
Hentati A, Deng H X, Hung W Y, et al. Human alpha-tocopherol transfer protein:gene structure and mutations in familial vitamin E deficiency. Annals of Neurology,1996,39(3):295-300
Hosomi A, Arita M, Sato Y, et al. Affinity for alpha-tocopherol transfer protein as a determinant of the biological activities of vitamin E analogs. Febs Letters,1997,409(1):105-108
Hosomi A, Goto K, Kondo H, et al. Localization of alpha-tocopherol transfer protein in rat brain. Neuroscience Letters,1998,256(3):159-162
Ikeda M, Kodama H, Fukuda J, et al. Role of radical oxygen species in rat testicular germ cell apoptosis induced by heat stress. Biology of Reproduction,1999,61(2):393-399
Israel K, Sanders B G, Kline K. RRR-alpha-tocopheryl succinate inhibits the proliferation of human prostatic tumor cells with defective cell cycle/differentiation pathways. Nutrition and Cancer-an International Journal,1995,24(2):161-169
Jefcoate C R, Artemenko I P, Zhao D. Relationship of StAR expression to mitochondrial cholesterol transfer and metabolism. Endocrine Research,2000,26(4):663-680
Jenkins S A, Birch S L, Atkinson P E. The effects of vitamin E-deficiency on serum prolaetin and serum luteinizing hormone levels in the pregnant rat. Cellular and Molecular Life Sciences,1980,36(2): 260-261
Jiang Q. Natural forms of vitamin E:metabolism, antioxidant, and anti-inflammatory activities and their role in disease prevention and therapy. Free Radical Biology and Medicine,2014, http://dx.doi.org/10.1016/j.freeradbiomed.2014.03.035
Jishage K, Arita M, Igarashi K, et al. Alpha-tocopherol transfer protein is important for the normal development of placental labyrinthine trophoblasts in mice. Journal of Biological Chemistry,2001, 276(3):1669-1672
Jishage K, Tachibe T, Ito T, et al. Vitamin E is essential for mouse placentation but not for embryonic development itself. Biology of Reproduction,2005,73(5):983-987
Kaempf-Rotzoll D E, Horiguchi M, Hashiguchi K, et al. Human placental trophoblast cells express alpha-tocopherol transfer protein. Placenta,2003,24(5):439-444
Kaempf-Rotzoll D E, Igarashi K, Aoki J, et al. Alpha-tocopherol transfer protein is specifically localized at the implantation site of pregnant mouse uterus. Biology of Reproduction,2002,67(2):599-604
Kalender S, Uzun F G, Demir F, et al. Mercuric chloride-induced testicular toxicity in rats and the protective role of sodium selenite and vitamin E. Food and Chemical Toxicology,2013,55: 456-462
Kankofer M. Placental release/retention in cows and its relation to peroxidative damage of macromolecules. Reproduction in Domestic Animals,2002,37(1):27-30
Kim H S, Arai H, Arita M, et al. Effect of alpha-tocopherol status on alpha-tocopherol transfer protein expression and its messenger RNA level in rat liver. Free Radical Research,1998,28(1):87-92
Kishimoto M, Yano Y, Yajima S, et al. The inhibitory effect of vitamin E on 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone-induced lung tumorigenesis in mice based on the regulation of polyamine metabolism. Cancer Letters,1998,126(2):173-178
Klein E A, Lippman S M, Thompson I M, et al. The selenium and vitamin E cancer prevention trial. World Journal of Urology,2003,21(1):21-27
Lacasa D, Le Liepvre X, Ferre P, et al. Progesterone stimulates adipocyte determination and differentiation 1/sterol regulatory element-binding protein lc gene expression, potential mechanism for the lipogenic effect of progesterone in adipose tissue. Journal of Biological Chemistry,2001,276(15):11512-11516
Leonard S W, Terasawa Y, Farese R J, et al. Incorporation of deuterated RRR-or all-rac-alpha-tocopherol in plasma and tissues of alpha-tocopherol transfer protein--null mice. American Journal of Clinical Nutrition,2002,75(3):555-560
Liu K, Luo H L, Yue D B, et al. Molecular cloning and characterization of the sheep alpha-TTP gene and its expression in response to different vitamin E status. Gene,2012,494(2):225-230
Liu M Y, Lai H Y, Yang B C, et al. The inhibitory effects of lead on steroidogenesis in MA-10 mouse Leydig tumor cells. Life Sciences,2001,68(8):849-859
Lukyanov K, Diatchenko L, Chenchik A, et al. Construction of cDNA libraries from small amounts of total RNA using the suppression PCR effect. Biochemical and Biophysical Research Communications,1997,230(2):285-288
Mahoney C W, Azzi A. Vitamin E inhibits protein kinase C activity. Biochemical and Biophysical Research Communications,1988,154(2):694-697
Maruyama K, Sugano S. Oligo-capping:a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. Gene,1994,138(1-2):171-174
McCoard S A, Wise T H, Fahrenkrug S C, et al. Temporal and spatial localization patterns of Gata4 during porcine gonadogenesis. Biology of Reproduction,2001,65(2):366-374
McCord J M. Superoxide, superoxide dismutase and oxygen toxicity. Review Biochemical Toxicology, 1979,1:109-124
Meier R, Tomizaki T, Schulze-Briese C, et al. The molecular basis of vitamin E retention:structure of human alpha-tocopherol transfer protein. Journal of Molecular Biology,2003,331(3):725-734
Meydani S N, Barklund M P, Liu S, et al. Vitamin E supplementation enhances cell-mediated immunity in healthy elderly subjects. American Journal of Clinical Nutrition,1990,52(3):557-563
Meydani S N, Han S N, Wu D. Vitamin E and immune response in the aged:molecular mechanisms and clinical implications. Immunological Reviews,2005,205(269-284
Miller W L. StAR search--what we know about how the steroidogenic acute regulatory protein mediates mitochondrial cholesterol import. Molecular Endocrinology,2007,21(3):589-601
Monahan F J, Buckley D J, Gray J I, et al. Effect of dietary Vitamin E on the stability of raw and cooked pork. Meat Science,1990,27(2):99-108
Mukai K, Nishimura M, Ishizu K, et al. Kinetic study of the reaction of vitamin C with vitamin E radicals (tocopheroxyls) in solution. Biochimica Et Biophysica Acta,1989,991(2):276-279
Mukai K, Watanabe Y, Uemoto Y, et al. Stopped-fl ow investigation of antioxidant activity of tocopherols. Bulletin of the Chemical Society of Japan,1986,59:3113-3116
Murugesan P, Muthusamy T, Balasubramanian K, et al. Effects of vitamins C and E on steroidogenic enzymes mRNA expression in polychlorinated biphenyl (Aroclor 1254) exposed adult rat Leydig cells. Toxicology,2007,232(3):170-182
Nier B, Weinberg P D, Rimbach G, et al. Differential gene expression in skeletal muscle of rats with vitamin E deficiency. Iubmb Life,2006,58(9):540-548
Niki E, Noguchi N, Gotoh N. Dynamics of lipid peroxidation and its inhibition by antioxidants. Biochemical Society Transactions,1993,21:313-317
Niki E, Tsuchiya Y, Yoshikawa Y, et al. Oxidation of lipids. XIII. Antioxidant activities of α-, β-, γ-, and δ-tocopherols. Bulletin of the Chemical Society of Japan,1986,59:497-501
Nishida Y, Yokota T, Takahashi T, et al. Deletion of vitamin E enhances phenotype of Alzheimer disease model mouse. Biochemical and Biophysical Research Communications,2006,350(3):530-536
Oda S S, E1-Maddawy Z. Protective effect of vitamin E and selenium combination on deltamethrin-induced reproductive toxicity in male rats. Experimental and Toxicologic Pathology, 2012,64(7-8):813-819
Olson SE, Seidel G E. Culture of in vitro-produced bovine embryos with vitamin E improves development in vitro and after transfer to recipients. Biology of Reproduction,2000,62(2): 248-252
Oommen S, Vasu V T, Leonard S W, et al. Genome wide responses of murine lungs to dietary alpha-tocopherol. Free Radical Resesrch,2007,41(1):98-109
Otero P, Bonet B, Hen-era E, et al. Development of atherosclerosis in the diabetic BALB/c mice. Prevention with Vitamin E administration. Atherosclerosis,2005,182(2):259-265
Ottino P, Duncan J R. Effect of alpha-tocopherol succinate on free radical and lipid peroxidation levels in BL6 melanoma cells. Free Radical Biology and Medicine,1997,22(7):1145-1151
Ouahchi K, Arita M, Kayden H, et al. Ataxia with isolated vitamin E deficiency is caused by mutations in the alpha-tocopherol transfer protein. Nature Genetics,1995,9(2):141-145
Payne A H, Youngblood G L. Regulation of expression of steroidogenic enzymes in Leydig cells. Biology of Reproduction,1995,52(2):217-225
Prange-Kjel J, Rune G M. Dietary and indirect effects of estrogen on rat hip-pocampus. Neuroscience,
2006,138(3):765-772
Pryor W A, Strickland T, Church D F. Comparison of the efficiencies of several natural and synthetic antioxidants in aqueous SDS micelle solutions. Journal of the American Chemical Society,1988, 110:2224-2229
Quinn P J. Is the distribution of alpha-tocopherol in membranes consistent with its putative functions? Biochemistry (Mosc),2004,69(1):58-66 Rao M V, Chawla S L, Sharma S R. Protective role of vitamin E on nickel and/or chromium induced oxidative stress in the mouse ovary. Food and Chemical Toxicology,2009,47(6):1368-1371
Rigotti A. Absorption, transport, and tissue delivery of vitamin E. Molecular Aspects of Medicine,2007, 28(5-6):423-436
Rizzo C, Dionisi-Vici C, D'Ippoliti M, et al. A simple and rapid HPLC method for simultaneous determination of plasma 7-dehydrocholesterol and vitamin E:its application in Smith-Lemli-Opitz patients. Clinica Chimica Acta,2000,291(1):97-102
Rota C, Barella L, Minihane A M, et al. Dietary alpha-tocopherol affects differential gene expression in rat testes. Iubmb Life,2004,56(5):277-280
Rota C, Rimbach G, Minihane A M, et al. Dietary vitamin E modulates differential gene expression in the rat hippocampus:potential implications for its neuroprotective properties. Nutritional Neuroscience,2005,8(1):21-29
Rotzoll D E, Scherling R, Etzl R, et al. Immunohistochemical localization of alpha-tocopherol transfer protein and lipoperoxidation products in human first-trimester and term placenta. European Journal of Obstetrics Gynecology and Reproductive Biology,2008,140(2):183-191
Sato Y, Arai H, Miyata A, et al. Primary structure of alpha-tocopherol transfer protein from rat liver. Homology with cellular retinaldehyde-binding protein. Journal of Biological Chemistry,1993, 268(24):17705-17710
Sato Y, Hagiwara K, Arai H, et al. Purification and characterization of the alpha-tocopherol transfer protein from rat liver. Febs Letters,1991,288(1-2):41-45
Scherf H, Machlin U, Frye TM, el al. Vitamin E biopotency:comparison of various 'natural-derived' and chemically synthesized a-tocopherols. Animal Food Science Technology,1996,59:115-126
Schmidt W M, Mueller M W. CapSelect:a highly sensitive method for 5' CAP-dependent enrichment of full-length cDNA in PCR-mediated analysis of mRNAs. Nucleic Acids Research,1999,27(21): e31
Schuelke M, Elsner A, Finckh B, et al. Urinary alpha-tocopherol metabolites in alpha-tocopherol transfer protein-deficient patients. Journal of Lipid Research,2000,41(10):1543-1551
Selvakumar K, Sheerin B L, Krishnamoorthy G, et al. Differential expression of androgen and estrogen receptors in PCB (Aroclor 1254)-exposed rat ventral prostate:impact of alpha-tocopherol. Experimental and Toxicologic Pathology,2011,63(1-2):105-112
Shargorodsky M, Debby O, Matas Z, et al. Effect of long-term treatment with antioxidants (vitamin C, vitamin E, coenzyme Q10 and selenium) on arterial compliance, humoral factors and inflammatory markers in patients with multiple cardiovascular risk factors. Nutrition Metabolism (Lond),2010,7: 55
Shaw H M, Huang C. Liver alpha-tocopherol transfer protein and its mRNA are differentially altered by dietary vitamin E deficiency and protein insufficiency in rats. Journal of Nutrition,1998,128(12): 2348-2354
Sigounas G, Anagnostou A, Steiner M. dl-alpha-tocopherol induces apoptosis in erythroleukemia, prostate, and breast cancer cells. Nutrition and Cancer-an International Journal,1997,28(1):30-35
Sirikci O, Ozer N K, Azzi A. Dietary cholesterol-induced changes of protein kinase C and the effect of vitamin E in rabbit aortic smooth muscle cells. Atherosclerosis,1996,126(2):253-263
Splan R K, Pilcik B R, Tomsen U J, et al. Rapid communication:mapping of the Steroidogenic Acute Regulatory Protein (StAR) gene to porcine chromosome 15 by linkage analysis using a novel PCR-RFLP. Journal of Animal Science,1998,76(2):658-659
Stocco D M. A StAR search:implications in controlling steroidgenesis. Biology of Reproduction,1997, 56(2):328-336
Stocco D M. Intramitochondrial cholesterol transfer. Biochimica Et Biophysica Acta,2000,1486(1): 184-197
Tamai H, Kim H S, Arai H, et al. Developmental changes in the expression of alpha-tocopherol transfer protein during the neonatal period of rat. Biofactors,1998,7(1-2):87-91
Terasawa Y, Ladha Z, Leonard S W, et al. Increased atherosclerosis in hyperlipidemic mice deficient in alpha -tocopherol transfer protein and vitamin E. Proceedings of the National Academy of Sciences U S A,2000,97(25):13830-13834
Thakur V, Morley S, and Manor D. Hepatic alpha-tocopherol transfer protein:ligand-induced protection from proteasomal degradation. Biochemistry,2010,49:9339-9344
Traber M G, Kayden H J. Vitamin E is delivered to cells via the high affinity receptor for low-density lipoprotein. American Journal of Clinical Nutrition,1984,40(4):747-751
Traber M G. Vitamin E regulatory mechanisms. Annual Review of Nutrition,2007,27:347-362
Tremblay J J, Hamel F, Viger R S. Protein kinase A-dependent cooperation between GATA and CCAAT/enhancer-binding protein transcription factors regulates steroidogenic acute regulatory protein promoter activity. Endocrinology,2002,143(10):3935-3945
Tremblay J J, Viger R S. A mutated form of steroidogenic factor 1 (SF-1 G35E) that causes sex reversal in humans fails to synergize with transcription factor GATA-4. Journal of Biological Chemistry, 2003,278(43):42637-42642
Tsujii H, Muranaka M, Hamano K. Culture of In Vitro Mouse Embryos with Vitamin E Improves Development. Journal of Reproduction and Development,2002,48:25-29
Ulatowski L, Dreussi C, Noy N, et al. Expression of the alpha-tocopherol transfer protein gene is regulated by oxidative stress and common single-nucleotide polymorphisms. Free Radical Biology and Medicine,2012,53(12):2318-2326
Ulatowski L, Manor D. Expression of the tocopherol transfer protein is regulated by oxidative stress. Free Radical Biology and Medicine,2010,49:S136-S136
Uzunhisarcikli M, Kalender Y, Dirican K, et al. Acute, subacute and subchronic administration of methyl parathion-induced testicular damage in male rats and protective role of vitamins C and E. Pesticide Biochemistry and Physiology,2007,87:115-122
Valacchi G, Vasu V T, Yokohama W, et al. Lung vitamin E transport processes are affected by both age and environmental oxidants in mice. Toxicology and Applied Pharmacology,2007,222(2): 227-234
Van Soest P J, Robertson J B, Lewis B A. Methods of dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science,1991,74: 3583-3597
Vasu V T, Hobson B, Gohil K, et al. Genome-wide screening of alpha-tocopherol sensitive genes in heart tissue from alpha-tocopherol transfer protein null mice (ATTP(-/-)). Febs Letters,2007, 581(8):1572-1578
Viana M, Herrera E, Bonet B. Teratogenic effects of diabetes mellitus in the rat. Prevention by vitamin E. Diabetologia,1996,39(9):1041-1046
Walker W H. Testosterone signaling and the regulation of spermatogenesis. Spermatogenesis,2011,1(2): 116-120
Walsh L P, Webster D R, Stocco D M. Dimethoate inhibits steroidogenesis by disrupting transcription of the steroidogenic acute regulatory (StAR) gene. Journal of Endocrinology,2000,167(2):253-263
Wang Y H, Byrne K A, Reverter A, et al. Transcriptional profiling of skeletal muscle tissue from two breeds of cattle. Mammalian Genome,2005,16(3):201-210
Watanabe T, Vassallo-Agius R. Broodstock nutrition research on marine finfish in Japan. Aquaculture, 2003,227:35-61
Waylan A T, Quinn P R, Unruh J A, et al. Effects of modified tall oil and vitamin E on growth performance, carcass characteristics, and meat quality of growing-finishing pigs. Journal of Animal Science,2002,80:1575-1576
Wei Y, Lu Z, Yuan C, et al. Molecular electronics:strategies and progress in China. IEEE Engineering in Medicine and Biology Magazine,1997,16(4):53-61
Wellenreuther R, Schupp I, Poustka A, et al. SMART amplification combined with cDNA size fractionation in order to obtain large full-length clones. BMC Genomics,2004,5(1):36
Whitworth K, Springer G K, Forrester L J, et al. Developmental expression of 2489 gene clusters during pig embryogenesis:an expressed sequence tag project. Biology of Reproduction,2004,71(4): 1230-1243
Yamaoka S, Kim H S, Ogihara T, et al. Severe Vitamin E deficiency exacerbates acute hyperoxic lung injury associated with increased oxidative stress and inflammation. Free Radical Research,2008, 42(6):602-612
Yan L, Yue D, Luo H, et al. Effect of Vitamin E supplementation on the enzymatic activity of selected markers in Aohan fine-wool sheep testis. Animal Reproduction Science,2010,122(3-4):264-269
Yokota T, Igarashi K, Uchihara T, et al. Delayed-onset ataxia in mice lacking alpha-tocopherol transfer protein:model for neuronal degeneration caused by chronic oxidative stress. Proceedings of the National Academy of Sciences U S A,2001,98(26):15185-15190
Yoshida Y, Itoh N, Hayakawa M, et al. Lipid peroxidation induced by carbon tetrachloride and its inhibition by antioxidant as evaluated by an oxidative stress marker, HODE. Toxicology and Applied Pharmacology,2005,208(1):87-97
Yoshida Y, Niki E, Noguchi N. Comparative study on the action of tocopherols and tocotrienols as antioxidant:chemical and physical effects. Chemistry and Physics of Lipids,2003,123:63-75
Yue D, Yan L, Luo H, et al. Effect of Vitamin E supplementation on semen quality and the testicular cell membranal and mitochondrial antioxidant abilities in Aohan fine-wool sheep. Animal Reproduction Science,2010,118(2-4):217-222
Zhang Q, Bai Q, Yuan Y, et al. Assessment of seminal estradiol and testosterone levels as predictors of human spermatogenesis. Journal of Andrology,2010,31(2):215-220
Zhu H, Luo H L, Meng H, et al. Effect of vitamin E supplementation on development of reproductive organs in Boer goat. Animal Reproduction Science,2009,113(1-4):93-101
Zhu H, Luo H L, Meng H, et al. Effect of vitamin E supplement in diet on antioxidant ability of testis in Boer goat. Animal Reproduction Science,2010,117(1-2):90-94
Zingg J M, Azzi A. Non-antioxidant activities of vitamin E. Current Medicinal Chemistry,2004,11(9): 1113-1133
Zingg J M. Modulation of signal transduction by vitamin E. Molecular Aspects of Medicine,2007, 28(5-6):481-506
Zingg J M. Vitamin E:an overview of major research directions. Molecular Aspects of Medicine,2007, 28(5-6):400-422
邓效禹,宿甲子,杨焕民,等.籽鹅垂体组织全长cDNA文库的构建及部分克隆序列分析.中国兽医学报,2011,31(4)：544-547
董重阳,康波,贾晓剑,等.籽鹅卵巢组织全长cDNA文库的构建及部分克隆序列分析.农业生物技术学报,2010,18(2)：389-393
冯迎春,颜其贵,郭万柱,等.山羊地方性鼻内肿瘤病毒ENTV-SC株基因组cDNA文库的构建及生物信息学分析.中国兽医科学,2011,41(2)：126-130
郭俣,刘长青,陆涛峰,等.北京油鸡cDNA文库的构建及purH基因的表达.南京农业大学学报,2010,33(4)：93-99
何震,谢玉波,肖强.人正常胃黏膜细胞全长cDNA文库的构建及鉴定.成都医学院学报,2010,5(1)：8-11
胡兰,孙长勉,刘梅.维生素E对肉仔鸡抗热应激的影响.沈阳农业大学学报,2001,32(2)：117-119
胡文琴,孟庆利,王恬.维生素E对畜禽繁殖性能的影响.当代畜禽养殖业,2004,(5)：15-16
贾金龙.兴凯湖梅花鹿肝脏全长cDNA文库的构建及鉴定：[硕士学位论文].北京：中国农业科学院,2007
孔亚坤,耿秀琴,尹俊杰,等.维生素E对老年雌性大鼠雌激素及海马芳香化酶表达的影响.中国老年学杂志,2009,29：1058-1060
李靖,徐晓霞,韩本立,等.正常肝组织与肝癌组织差异表达基因消减cDNA文库的构建.中华肝脏病杂志,2001,9(6)：346-348
李苏.不同剂量维生素E对更年期大鼠卵巢功能的影响：[硕士学位论文].济南：山东大学,2008
李鑫,邹毅辉,杨晓亮,等.中国美利奴羊外周免疫器官cDNA文库的构建及鉴定.石河子大学学报,2010,28(5)：529-533
梁明振,卢克焕,刘芳.饲粮锌、硒和维生素E水平对经产母猪繁殖性能及相关参数的影响.中国兽医学报,2003,23(6)：620-623
刘洪波,罗海玲,刘志宏,等.日粮维生素E水平对山羊精液品质的影响.中国畜牧杂志,2005,41(10)：12-14
刘坡,李凯彬,王芳,等.剑尾鱼肝脏cDNA文库的构建.畜牧与兽医,2007,39(5)：12-15
刘学剑.维生素E的生理功能与动物生产.江西饲料,2000(3)：16-17
柳淑芳.小尾寒羊多胎性状相关基因的鉴定和遗传研究：[博士学位论文].泰安：山东农业大学,2004
卢圣栋.现代分子生物学实验技术(第二版).北京：中国协和医科大学,1999
陆涛峰.鄂伦春马cDNA文库构建及ccLZ基因的克隆与表达研究：[硕士学位论文].北京：中国农业科学院,2008
罗海玲,贾志海,朱士恩,等.稀释液中添加维生素E对绵羊冷冻精液品质的影响.中国畜牧杂 志,2004,(11)：7-9
罗海玲,孟慧,朱虹,等.维生素E改善羊肉品质的机理初探.饲料工业,2010(S2)：57-63
马金彪.条锈菌诱导的小麦叶片cDNA文库构建及表达序列标签分析：[硕士学位论文].陕西：西北农林科技大学,2006
蒲俊华,王强.维生素A和维生素E对母畜繁殖性能的影响.畜牧与饲料科学,2006,3：51-52
秦胜芳,谭卫东,陈又南,等.恒河猴肝脏cDNA文库的构建.四川大学学报(医学版),2007,38(3)：378-381
沈倍奋.分子文库.北京：科学出版社,2001
石新辉,边连全,王娜.肥育后期添加VE毗啶羧酸铬对肥育猪肉品品质的影响.畜牧兽医杂志,2005,24(6)：1-4
史清河.维生素E对肉品质的改善作用.中国饲料,1999,3：9-12
孙佳音,应锋,韩晓冬.睾丸间质细胞中睾酮合成酶及蛋白表达的调控因子.生殖与避孕,2009,29(1)：42-47
孙永风,李立勇,武明宇.维生素E营养研究进展.现代农业科技,2007,23：209-210
王彩玲,苗军.蛋鸡热应激的营养疗法.中国家禽,2002(6)：40-41
王传蓉.维生素E和硒对荷斯坦母牛及新生犊牛的影响：[硕士学位论文].江苏：扬州大学,2008
汪开毓,周震,耿毅.鲤维生素E缺乏的病理学研究.内蒙古农业大学学报,2005,27：165-166
王丽.围产期奶牛日粮添加不同水平维生素E对其生产性能、免疫功能及繁殖机能的影响：[硕士学位论文].内蒙古：内蒙古农业大学,2010
王秀利,李宁,赵志辉,等.香猪肌肉组织cDNA文库的构建及其EST测序成功率的分析.遗传,2002,24(3)：263-266
王秀利,李宁,赵兴波,等.丝毛乌骨鸡胸肌和肝脏cDNA文库的构建与鉴定.农业生物技术学报,2003,11(1)：60-63
王远志,陈创夫,盛金良,等.绵羊种布鲁氏菌019株侵染绵羊巨噬细胞cDNA文库的构建.中国人兽共患病学报,2008,24(4)：334-337
吴伟,徐日福,高光,等.鹅肌肉组织全长cDNA文库构建及泛素基因序列分析.畜牧与兽医,2007,39(5)：12-15
徐日福,吴伟,高光,等.鹅肝脏组织的质粒cDNA文库构建.吉林农业大学学报,2007,29(3)：325-328
闫顺,李付美,曲志才,等.莱芜猪肝脏组织全长cDNA文库的构建和鉴定.生物技术通报,2010,21(3)：397-400
杨波涛,陈凤香,莫文莲,等.我国食用植物油维生素E含量研究.粮油加工,2009(9)：52-55
杨畅,方福德.基因芯片数据分析.生命科学,2004,16(2)：41-48
杨丽萍,杨慧荣,张勇.中国大鲵脑cDNA文库构建及促甲状腺激素β亚基基因cDNA的克隆和序列分析.水产学报,2005,32(4)：507-516
岳度兵.维生素E对生长期敖汉细毛羊精液品质的影响及作用机理：[硕士学位论文].北京：中国农业大学,2009
张锦前,牛俊奇,王峰.人类肝脏cDNA文库的构建及hHGF基因的筛选、克隆与表达.生物医 学工程与临床,2006,10(5)：315-319
张亮,史雨红,陈炯,等.香鱼肝胰腺cDNA文库的构建和鉴定.水产科学,2011,30(1)：11-16
郑景洲,许友卿,丁兆坤.维生素E的功能、吸收与代谢(2).生物学通报,2005,40(12)：1-2
郑鹏然,周树南.食品卫生全书.北京：红旗出版社,1996
周显青,孙儒泳,牛翠娟.维生素E对免疫功能的影响.生理科学进展,2000(2)：163-165
朱虹.维生素E对波尔山羊公羔生殖器官抗氧化性能及其发育的影响：[硕士学位论文].北京：中国农业大学,2008
Abdala-Valencia H, Berdnikovs S, Cook-Mills J M. Vitamin E isoforms differentially regulate intercellular adhesion molecule-1 activation of PKCalpha in human microvascular endothelial cells. PLoS One,2012,7(7):e41054-e41058
Acharya U R, Mishra M, Mishra I, et al. Potential role of vitamins in chromium induced spermatogenesis in Swiss mice. Environmental Toxicology and Pharmacology,2004,15(2-3): 53-59
Acharya U R, Mishra M, Patro J, et al. Effect of vitamins C and E on spermatogenesis in mice exposed to cadmium. Reproductive Toxicology,2008,25(1):84-88
Adamstone F B. The effects of vitamin-E deficiency on the development of the chick. Journal of Morphology,2005,52(1):47-89
Anthony D Q. Evaluating the effects of maternal and progeny dietary supplementation of selenium yeast and vitamin E on the performance of broiler-breeder hens and performance and meat quality of progeny. The U S:University of Kentucky,2012
Adams M D, Kelley J M, Gocayne J D, et al. Complementary DNA sequencing:expressed sequence tags and human genome project. Science,1991,252(5013):1651-1656
AOAC. Official methods of analysis,5th ed. Washington:Association of Official Analytical Chemist, 1990
Arita M, Sato Y, Miyata A, et al. Human alpha-tocopherol transfer protein:cDNA cloning, expression and chromosomal localization. Biochemical Journal,1995,306 (Pt 2):437-443
Atkinson J, Epand R F, Epand R M. Tocopherols and tocotrienols in membranes:a critical review. Free Radical Biology and Medicine,2008,44(5):739-764
Aytan N, Jung T, Tamturk F, et al. Oxidative stress related changes in the brain of hypercholesterolemic rabbits. Biofactors,2008,33(3):225-236
Ban R, Takitani K, Kim H S, et al. alpha-Tocopherol transfer protein expression in rat liver exposed to hyperoxia. Free Radical Research,2002,36(9):933-938
Barella L, Muller P Y, Schlachter M, et al. Identification of hepatic molecular mechanisms of action of alpha-tocopherol using global gene expression profile analysis in rats. Biochimica Et Biophysica Acta,2004,1689(1):66-74
Bauernfeind J C, Rubin S H, Surmatis J D, et al. Carotenoids and fat-soluble vitamins:contribution to food, feed and Pharmaceuticals. Int Z Vitaminforsch,1970,40(3):391-416
Bella D L, Schock B C, Lim Y, et al. Regulation of the alpha-tocopherol transfer protein in mice:lack of response to dietary vitamin E or oxidative stress. Lipids,2006,41(2):105-112
Boscoboinik D, Szewczyk A, Azzi A. Alpha-tocopherol (vitamin E) regulates vascular smooth muscle cell proliferation and protein kinase C activity. Archives of Biochemistry and Biophysics,1991, 286(1):264-269
Boscoboinik D, Szewczyk A, Hensey C, et al. Inhibition of cell proliferation by alpha-tocopherol. Role of protein kinase C. Journal of Biological Chemistry,1991,266(10):6188-6194
Bozkurt M, Abuk M C, Alcicek A. Effect of dietary fat type on broiler breeder performance and
hatching egg characteristics. Journal of Applied Poultry Research,2008,17(1):47-53
Brigelius-Flohe R, Kluth D, and Banning A. Is there a future for antioxidants in atherogenesis? Molecular nutrition & food research,2005,49:1083-1089
Brigelius-Flohe R, Traber M G. Vitamin E:function and metabolism. Faseb Journal,1999,13(10): 1145-1155
Burton G W, Doba T, Gabe E J, et al. Autoxidation of biological molecules.4. Maximizing the antioxidant activity of phenols. Journal of the American Chemical Society,1985,107(24): 7053-7065
Burton G W, Traber M G, Acuff R V, et al. Human plasma and tissue a-tocopherol concentrations in response to supplementation with deuterated natural and synthetic vitamin E. American Journal of Clinical Nutrition,1998,67(4):669-684
Capper J L, Wilkinson R G, Kasapidou E, et al. The effect of dietary vitamin E and fatty acid
supplementation of pregnant and lactating ewes on placental and mammary transfer of vitamin E to
the lamb. British Journal of Nutrition,2005,93(4):549-557
Catalgol B, Kartal O N. Lipid rafts and redox regulation of cellular signaling in cholesterol induced atherosclerosis. Current Cardiology Reviews,2010,6(4):309-324
Cavalier L, Ouahchi K, Kayden H J, et al. Ataxia with isolated vitamin E deficiency:heterogeneity of mutations and phenotypic variability in a large number of families. American Journal of Human Genetics,1998,62(2):301-310
Chandra A K, Chatterjee A, Ghosh R, et al. Vitamin E-supplementation protect chromium (Ⅵ)-induced spermatogenic and steroidogenic disorders in testicular tissues of rats. Food and Chemical Toxicology,2010,48(3):972-979
Cherian G, Sim J S. Egg yolk polyunsaturated fatty acids and vitamin E content alters the tocopherol status of hatched chicks. Poultry Science,1997,76(12):1753-1759
Chiku S, Hamamura K, Nakamura T. Novel urinary metabolite of d-delta-tocopherol in rats. Journal of Lipid Research,1984,25(1):40-48
Cooperr D R, Kling O R, Carpenter M P. Effect of vitamin E deficiency on serum concentrations of follicle-stimulating hormone and testosterone during testicular maturation and degeneration.
Endocrinology,1987,120(1):83-90
Doria-Lamba L, De Grandis E, Cristiani E, et al. Efficacious vitamin E treatment in a child with ataxia with isolated vitamin E deficiency. European Journal of Pediatrics,2006,165(7):494-495
Du XJ, Wang J X, Liu N, et al. Identification and molecular characterization of a peritrophin-like protein from fleshy prawn (Fenneropenaeus chinensis). Molecular Immunology,2006,43(10): 1633-1644
Edery I, Chu L L, Sonenberg N, et al. An efficient strategy to isolate full-length cDNAs based on an mRNA cap retention procedure (CAPture). Molecular and Cellular Biology,1995,15(6): 3363-3371
Efimov V A, Chakhmakhcheva O G, Archdeacon J, et al. Detection of the 5'-cap structure of messenger RNAs with the use of the cap-jumping approach. Nucleic Acids Research,2001,29(22): 4751-4759
Evans H M, Bishop K. S. On the existence of a hitherto unrecognized dietary factor essential for reproduction. Science,1922,56(1458):650-651
Fechner H, Schlame M, Guthmann F, et al. alpha- and delta-tocopherol induce expression of hepatic alpha-tocopherol-transfer-protein mRNA. Biochemical Journal,1998,331 (Pt 2):577-581
Fernandez-Palacios H, Izquierdo M S, Gonzalez M, et al. Combined effect of dietary a-tocopherol and
n-3 HUFA on egg quality of gilthead seabream (Sparus aurata). Aquaculture,1998,161:475-476
Fischer A, Pallauf J, Gohil K, et al. Effect of selenium and vitamin E deficiency on differential gene expression in rat liver. Biochemical and Biophysical Research Communications,2001,285: 470-475
Fridovich I. Biological effects of the superoxide radical. Archives of Biochemistry and Biophysics, 1986,247(1):1-11
Fruebis J, Silvestre M, Shelton D, et al. Inhibition of VCAM-1 expression in the arterial wall is shared by structurally different antioxidants that reduce early atherosclerosis in NZW rabbits. Journal of Lipid Research,1999,40(11):1958-1966
Gabsi S, Gouider-Khouja N, Belal S, et al. Effect of vitamin E supplementation in patients with ataxia with vitamin E deficiency. European Journal of Neurology,2001,8(5):477-481
Gamal B M, Sherief M A, Hassan A H. Effect of combination of vitamin E and selenium injections on reproductive performance and blood parameters of Ossimi rams. Small Ruminant Research,2013, 113:103-108
Gohil K, Godzdanker R, O'Roark E, et al. Alpha-tocopherol transfer protein deficiency in mice causes multi-organ deregulation of gene networks and behavioral deficits with age. Annals of the New York Academy of Sciences,2004,1031:109-126
Gohil K, Oommen S, Quach H T, et al. Mice lacking alpha-tocopherol transfer protein gene have severe alpha-tocopherol deficiency in multiple regions of the central nervous system. Brain Research, 2008,1201:167-176
Grobas S, Mendez J, Lopez B C, et al. Effect of vitamin E and A supplementation on egg yolk alpha-tocopherol concentration. Poultry Science,2002,81(3):376-381
Hatfield P G, Robinson B L, Minikhiem D L, et al. Serum alpha-tocopherol and immune function in yearling ewes supplemented with zinc and vitamin E. Journal of Animal Science,2002,80(5): 1329-1334
Heemers H, Maes B, Foufelle F, et al. Androgens stimulate lipogenic gene expression in prostate cancer cells by activation of the sterol regulatory element-binding protein cleavage activating protein/sterol regulatory element-binding protein pathway. Molecular Endocrinology,2001,15(10): 1817-1828
Heemers H, Vanderhoydonc F, Roskams T, et al. Androgens stimulate coordinated lipogenic gene expression in normal target tissues in vivo. Molecular and Cellular Endocrinology,2003,205(1-2): 21-31
Hensley K, Benaksas E J, Bolli R, et al. New perspectives on vitamin E:c-tocopherol and carboxyelthylhydroxychroman metabolites in biology and medicine. Free Radical Biology and Medicine,2004,36(1):1-15
Hentati A, Deng H X, Hung W Y, et al. Human alpha-tocopherol transfer protein:gene structure and mutations in familial vitamin E deficiency. Annals of Neurology,1996,39(3):295-300
Hosomi A, Arita M, Sato Y, et al. Affinity for alpha-tocopherol transfer protein as a determinant of the biological activities of vitamin E analogs. Febs Letters,1997,409(1):105-108
Hosomi A, Goto K, Kondo H, et al. Localization of alpha-tocopherol transfer protein in rat brain. Neuroscience Letters,1998,256(3):159-162
Ikeda M, Kodama H, Fukuda J, et al. Role of radical oxygen species in rat testicular germ cell apoptosis induced by heat stress. Biology of Reproduction,1999,61(2):393-399
Israel K, Sanders B G, Kline K. RRR-alpha-tocopheryl succinate inhibits the proliferation of human prostatic tumor cells with defective cell cycle/differentiation pathways. Nutrition and Cancer-an International Journal,1995,24(2):161-169
Jefcoate C R, Artemenko I P, Zhao D. Relationship of StAR expression to mitochondrial cholesterol transfer and metabolism. Endocrine Research,2000,26(4):663-680
Jenkins S A, Birch S L, Atkinson P E. The effects of vitamin E-deficiency on serum prolaetin and serum luteinizing hormone levels in the pregnant rat. Cellular and Molecular Life Sciences,1980,36(2): 260-261
Jiang Q. Natural forms of vitamin E:metabolism, antioxidant, and anti-inflammatory activities and their role in disease prevention and therapy. Free Radical Biology and Medicine,2014, http://dx.doi.org/10.1016/j.freeradbiomed.2014.03.035
Jishage K, Arita M, Igarashi K, et al. Alpha-tocopherol transfer protein is important for the normal development of placental labyrinthine trophoblasts in mice. Journal of Biological Chemistry,2001, 276(3):1669-1672
Jishage K, Tachibe T, Ito T, et al. Vitamin E is essential for mouse placentation but not for embryonic development itself. Biology of Reproduction,2005,73(5):983-987
Kaempf-Rotzoll D E, Horiguchi M, Hashiguchi K, et al. Human placental trophoblast cells express alpha-tocopherol transfer protein. Placenta,2003,24(5):439-444
Kaempf-Rotzoll D E, Igarashi K, Aoki J, et al. Alpha-tocopherol transfer protein is specifically localized at the implantation site of pregnant mouse uterus. Biology of Reproduction,2002,67(2):599-604
Kalender S, Uzun F G, Demir F, et al. Mercuric chloride-induced testicular toxicity in rats and the protective role of sodium selenite and vitamin E. Food and Chemical Toxicology,2013,55: 456-462
Kankofer M. Placental release/retention in cows and its relation to peroxidative damage of macromolecules. Reproduction in Domestic Animals,2002,37(1):27-30
Kim H S, Arai H, Arita M, et al. Effect of alpha-tocopherol status on alpha-tocopherol transfer protein expression and its messenger RNA level in rat liver. Free Radical Research,1998,28(1):87-92
Kishimoto M, Yano Y, Yajima S, et al. The inhibitory effect of vitamin E on 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone-induced lung tumorigenesis in mice based on the regulation of polyamine metabolism. Cancer Letters,1998,126(2):173-178
Klein E A, Lippman S M, Thompson I M, et al. The selenium and vitamin E cancer prevention trial. World Journal of Urology,2003,21(1):21-27
Lacasa D, Le Liepvre X, Ferre P, et al. Progesterone stimulates adipocyte determination and differentiation 1/sterol regulatory element-binding protein lc gene expression, potential mechanism for the lipogenic effect of progesterone in adipose tissue. Journal of Biological Chemistry,2001,276(15):11512-11516
Leonard S W, Terasawa Y, Farese R J, et al. Incorporation of deuterated RRR-or all-rac-alpha-tocopherol in plasma and tissues of alpha-tocopherol transfer protein--null mice. American Journal of Clinical Nutrition,2002,75(3):555-560
Liu K, Luo H L, Yue D B, et al. Molecular cloning and characterization of the sheep alpha-TTP gene and its expression in response to different vitamin E status. Gene,2012,494(2):225-230
Liu M Y, Lai H Y, Yang B C, et al. The inhibitory effects of lead on steroidogenesis in MA-10 mouse Leydig tumor cells. Life Sciences,2001,68(8):849-859
Lukyanov K, Diatchenko L, Chenchik A, et al. Construction of cDNA libraries from small amounts of total RNA using the suppression PCR effect. Biochemical and Biophysical Research Communications,1997,230(2):285-288
Mahoney C W, Azzi A. Vitamin E inhibits protein kinase C activity. Biochemical and Biophysical Research Communications,1988,154(2):694-697
Maruyama K, Sugano S. Oligo-capping:a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. Gene,1994,138(1-2):171-174
McCoard S A, Wise T H, Fahrenkrug S C, et al. Temporal and spatial localization patterns of Gata4 during porcine gonadogenesis. Biology of Reproduction,2001,65(2):366-374
McCord J M. Superoxide, superoxide dismutase and oxygen toxicity. Review Biochemical Toxicology, 1979,1:109-124
Meier R, Tomizaki T, Schulze-Briese C, et al. The molecular basis of vitamin E retention:structure of human alpha-tocopherol transfer protein. Journal of Molecular Biology,2003,331(3):725-734
Meydani S N, Barklund M P, Liu S, et al. Vitamin E supplementation enhances cell-mediated immunity in healthy elderly subjects. American Journal of Clinical Nutrition,1990,52(3):557-563
Meydani S N, Han S N, Wu D. Vitamin E and immune response in the aged:molecular mechanisms and clinical implications. Immunological Reviews,2005,205(269-284
Miller W L. StAR search--what we know about how the steroidogenic acute regulatory protein mediates mitochondrial cholesterol import. Molecular Endocrinology,2007,21(3):589-601
Monahan F J, Buckley D J, Gray J I, et al. Effect of dietary Vitamin E on the stability of raw and cooked pork. Meat Science,1990,27(2):99-108
Mukai K, Nishimura M, Ishizu K, et al. Kinetic study of the reaction of vitamin C with vitamin E radicals (tocopheroxyls) in solution. Biochimica Et Biophysica Acta,1989,991(2):276-279
Mukai K, Watanabe Y, Uemoto Y, et al. Stopped-fl ow investigation of antioxidant activity of tocopherols. Bulletin of the Chemical Society of Japan,1986,59:3113-3116
Murugesan P, Muthusamy T, Balasubramanian K, et al. Effects of vitamins C and E on steroidogenic enzymes mRNA expression in polychlorinated biphenyl (Aroclor 1254) exposed adult rat Leydig cells. Toxicology,2007,232(3):170-182
Nier B, Weinberg P D, Rimbach G, et al. Differential gene expression in skeletal muscle of rats with vitamin E deficiency. Iubmb Life,2006,58(9):540-548
Niki E, Noguchi N, Gotoh N. Dynamics of lipid peroxidation and its inhibition by antioxidants. Biochemical Society Transactions,1993,21:313-317
Niki E, Tsuchiya Y, Yoshikawa Y, et al. Oxidation of lipids. XIII. Antioxidant activities of α-, β-, γ-, and δ-tocopherols. Bulletin of the Chemical Society of Japan,1986,59:497-501
Nishida Y, Yokota T, Takahashi T, et al. Deletion of vitamin E enhances phenotype of Alzheimer disease model mouse. Biochemical and Biophysical Research Communications,2006,350(3):530-536
Oda S S, E1-Maddawy Z. Protective effect of vitamin E and selenium combination on deltamethrin-induced reproductive toxicity in male rats. Experimental and Toxicologic Pathology, 2012,64(7-8):813-819
Olson SE, Seidel G E. Culture of in vitro-produced bovine embryos with vitamin E improves development in vitro and after transfer to recipients. Biology of Reproduction,2000,62(2): 248-252
Oommen S, Vasu V T, Leonard S W, et al. Genome wide responses of murine lungs to dietary alpha-tocopherol. Free Radical Resesrch,2007,41(1):98-109
Otero P, Bonet B, Hen-era E, et al. Development of atherosclerosis in the diabetic BALB/c mice. Prevention with Vitamin E administration. Atherosclerosis,2005,182(2):259-265
Ottino P, Duncan J R. Effect of alpha-tocopherol succinate on free radical and lipid peroxidation levels in BL6 melanoma cells. Free Radical Biology and Medicine,1997,22(7):1145-1151
Ouahchi K, Arita M, Kayden H, et al. Ataxia with isolated vitamin E deficiency is caused by mutations in the alpha-tocopherol transfer protein. Nature Genetics,1995,9(2):141-145
Payne A H, Youngblood G L. Regulation of expression of steroidogenic enzymes in Leydig cells. Biology of Reproduction,1995,52(2):217-225
Prange-Kjel J, Rune G M. Dietary and indirect effects of estrogen on rat hip-pocampus. Neuroscience,
2006,138(3):765-772
Pryor W A, Strickland T, Church D F. Comparison of the efficiencies of several natural and synthetic antioxidants in aqueous SDS micelle solutions. Journal of the American Chemical Society,1988, 110:2224-2229
Quinn P J. Is the distribution of alpha-tocopherol in membranes consistent with its putative functions? Biochemistry (Mosc),2004,69(1):58-66 Rao M V, Chawla S L, Sharma S R. Protective role of vitamin E on nickel and/or chromium induced oxidative stress in the mouse ovary. Food and Chemical Toxicology,2009,47(6):1368-1371
Rigotti A. Absorption, transport, and tissue delivery of vitamin E. Molecular Aspects of Medicine,2007, 28(5-6):423-436
Rizzo C, Dionisi-Vici C, D'Ippoliti M, et al. A simple and rapid HPLC method for simultaneous determination of plasma 7-dehydrocholesterol and vitamin E:its application in Smith-Lemli-Opitz patients. Clinica Chimica Acta,2000,291(1):97-102
Rota C, Barella L, Minihane A M, et al. Dietary alpha-tocopherol affects differential gene expression in rat testes. Iubmb Life,2004,56(5):277-280
Rota C, Rimbach G, Minihane A M, et al. Dietary vitamin E modulates differential gene expression in the rat hippocampus:potential implications for its neuroprotective properties. Nutritional Neuroscience,2005,8(1):21-29
Rotzoll D E, Scherling R, Etzl R, et al. Immunohistochemical localization of alpha-tocopherol transfer protein and lipoperoxidation products in human first-trimester and term placenta. European Journal of Obstetrics Gynecology and Reproductive Biology,2008,140(2):183-191
Sato Y, Arai H, Miyata A, et al. Primary structure of alpha-tocopherol transfer protein from rat liver. Homology with cellular retinaldehyde-binding protein. Journal of Biological Chemistry,1993, 268(24):17705-17710
Sato Y, Hagiwara K, Arai H, et al. Purification and characterization of the alpha-tocopherol transfer protein from rat liver. Febs Letters,1991,288(1-2):41-45
Scherf H, Machlin U, Frye TM, el al. Vitamin E biopotency:comparison of various 'natural-derived' and chemically synthesized a-tocopherols. Animal Food Science Technology,1996,59:115-126
Schmidt W M, Mueller M W. CapSelect:a highly sensitive method for 5' CAP-dependent enrichment of full-length cDNA in PCR-mediated analysis of mRNAs. Nucleic Acids Research,1999,27(21): e31
Schuelke M, Elsner A, Finckh B, et al. Urinary alpha-tocopherol metabolites in alpha-tocopherol transfer protein-deficient patients. Journal of Lipid Research,2000,41(10):1543-1551
Selvakumar K, Sheerin B L, Krishnamoorthy G, et al. Differential expression of androgen and estrogen receptors in PCB (Aroclor 1254)-exposed rat ventral prostate:impact of alpha-tocopherol. Experimental and Toxicologic Pathology,2011,63(1-2):105-112
Shargorodsky M, Debby O, Matas Z, et al. Effect of long-term treatment with antioxidants (vitamin C, vitamin E, coenzyme Q10 and selenium) on arterial compliance, humoral factors and inflammatory markers in patients with multiple cardiovascular risk factors. Nutrition Metabolism (Lond),2010,7: 55
Shaw H M, Huang C. Liver alpha-tocopherol transfer protein and its mRNA are differentially altered by dietary vitamin E deficiency and protein insufficiency in rats. Journal of Nutrition,1998,128(12): 2348-2354
Sigounas G, Anagnostou A, Steiner M. dl-alpha-tocopherol induces apoptosis in erythroleukemia, prostate, and breast cancer cells. Nutrition and Cancer-an International Journal,1997,28(1):30-35
Sirikci O, Ozer N K, Azzi A. Dietary cholesterol-induced changes of protein kinase C and the effect of vitamin E in rabbit aortic smooth muscle cells. Atherosclerosis,1996,126(2):253-263
Splan R K, Pilcik B R, Tomsen U J, et al. Rapid communication:mapping of the Steroidogenic Acute Regulatory Protein (StAR) gene to porcine chromosome 15 by linkage analysis using a novel PCR-RFLP. Journal of Animal Science,1998,76(2):658-659
Stocco D M. A StAR search:implications in controlling steroidgenesis. Biology of Reproduction,1997, 56(2):328-336
Stocco D M. Intramitochondrial cholesterol transfer. Biochimica Et Biophysica Acta,2000,1486(1): 184-197
Tamai H, Kim H S, Arai H, et al. Developmental changes in the expression of alpha-tocopherol transfer protein during the neonatal period of rat. Biofactors,1998,7(1-2):87-91
Terasawa Y, Ladha Z, Leonard S W, et al. Increased atherosclerosis in hyperlipidemic mice deficient in alpha -tocopherol transfer protein and vitamin E. Proceedings of the National Academy of Sciences U S A,2000,97(25):13830-13834
Thakur V, Morley S, and Manor D. Hepatic alpha-tocopherol transfer protein:ligand-induced protection from proteasomal degradation. Biochemistry,2010,49:9339-9344
Traber M G, Kayden H J. Vitamin E is delivered to cells via the high affinity receptor for low-density lipoprotein. American Journal of Clinical Nutrition,1984,40(4):747-751
Traber M G. Vitamin E regulatory mechanisms. Annual Review of Nutrition,2007,27:347-362
Tremblay J J, Hamel F, Viger R S. Protein kinase A-dependent cooperation between GATA and CCAAT/enhancer-binding protein transcription factors regulates steroidogenic acute regulatory protein promoter activity. Endocrinology,2002,143(10):3935-3945
Tremblay J J, Viger R S. A mutated form of steroidogenic factor 1 (SF-1 G35E) that causes sex reversal in humans fails to synergize with transcription factor GATA-4. Journal of Biological Chemistry, 2003,278(43):42637-42642
Tsujii H, Muranaka M, Hamano K. Culture of In Vitro Mouse Embryos with Vitamin E Improves Development. Journal of Reproduction and Development,2002,48:25-29
Ulatowski L, Dreussi C, Noy N, et al. Expression of the alpha-tocopherol transfer protein gene is regulated by oxidative stress and common single-nucleotide polymorphisms. Free Radical Biology and Medicine,2012,53(12):2318-2326
Ulatowski L, Manor D. Expression of the tocopherol transfer protein is regulated by oxidative stress. Free Radical Biology and Medicine,2010,49:S136-S136
Uzunhisarcikli M, Kalender Y, Dirican K, et al. Acute, subacute and subchronic administration of methyl parathion-induced testicular damage in male rats and protective role of vitamins C and E. Pesticide Biochemistry and Physiology,2007,87:115-122
Valacchi G, Vasu V T, Yokohama W, et al. Lung vitamin E transport processes are affected by both age and environmental oxidants in mice. Toxicology and Applied Pharmacology,2007,222(2): 227-234
Van Soest P J, Robertson J B, Lewis B A. Methods of dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science,1991,74: 3583-3597
Vasu V T, Hobson B, Gohil K, et al. Genome-wide screening of alpha-tocopherol sensitive genes in heart tissue from alpha-tocopherol transfer protein null mice (ATTP(-/-)). Febs Letters,2007, 581(8):1572-1578
Viana M, Herrera E, Bonet B. Teratogenic effects of diabetes mellitus in the rat. Prevention by vitamin E. Diabetologia,1996,39(9):1041-1046
Walker W H. Testosterone signaling and the regulation of spermatogenesis. Spermatogenesis,2011,1(2): 116-120
Walsh L P, Webster D R, Stocco D M. Dimethoate inhibits steroidogenesis by disrupting transcription of the steroidogenic acute regulatory (StAR) gene. Journal of Endocrinology,2000,167(2):253-263
Wang Y H, Byrne K A, Reverter A, et al. Transcriptional profiling of skeletal muscle tissue from two breeds of cattle. Mammalian Genome,2005,16(3):201-210
Watanabe T, Vassallo-Agius R. Broodstock nutrition research on marine finfish in Japan. Aquaculture, 2003,227:35-61
Waylan A T, Quinn P R, Unruh J A, et al. Effects of modified tall oil and vitamin E on growth performance, carcass characteristics, and meat quality of growing-finishing pigs. Journal of Animal Science,2002,80:1575-1576
Wei Y, Lu Z, Yuan C, et al. Molecular electronics:strategies and progress in China. IEEE Engineering in Medicine and Biology Magazine,1997,16(4):53-61
Wellenreuther R, Schupp I, Poustka A, et al. SMART amplification combined with cDNA size fractionation in order to obtain large full-length clones. BMC Genomics,2004,5(1):36
Whitworth K, Springer G K, Forrester L J, et al. Developmental expression of 2489 gene clusters during pig embryogenesis:an expressed sequence tag project. Biology of Reproduction,2004,71(4): 1230-1243
Yamaoka S, Kim H S, Ogihara T, et al. Severe Vitamin E deficiency exacerbates acute hyperoxic lung injury associated with increased oxidative stress and inflammation. Free Radical Research,2008, 42(6):602-612
Yan L, Yue D, Luo H, et al. Effect of Vitamin E supplementation on the enzymatic activity of selected markers in Aohan fine-wool sheep testis. Animal Reproduction Science,2010,122(3-4):264-269
Yokota T, Igarashi K, Uchihara T, et al. Delayed-onset ataxia in mice lacking alpha-tocopherol transfer protein:model for neuronal degeneration caused by chronic oxidative stress. Proceedings of the National Academy of Sciences U S A,2001,98(26):15185-15190
Yoshida Y, Itoh N, Hayakawa M, et al. Lipid peroxidation induced by carbon tetrachloride and its inhibition by antioxidant as evaluated by an oxidative stress marker, HODE. Toxicology and Applied Pharmacology,2005,208(1):87-97
Yoshida Y, Niki E, Noguchi N. Comparative study on the action of tocopherols and tocotrienols as antioxidant:chemical and physical effects. Chemistry and Physics of Lipids,2003,123:63-75
Yue D, Yan L, Luo H, et al. Effect of Vitamin E supplementation on semen quality and the testicular cell membranal and mitochondrial antioxidant abilities in Aohan fine-wool sheep. Animal Reproduction Science,2010,118(2-4):217-222
Zhang Q, Bai Q, Yuan Y, et al. Assessment of seminal estradiol and testosterone levels as predictors of human spermatogenesis. Journal of Andrology,2010,31(2):215-220
Zhu H, Luo H L, Meng H, et al. Effect of vitamin E supplementation on development of reproductive organs in Boer goat. Animal Reproduction Science,2009,113(1-4):93-101
Zhu H, Luo H L, Meng H, et al. Effect of vitamin E supplement in diet on antioxidant ability of testis in Boer goat. Animal Reproduction Science,2010,117(1-2):90-94
Zingg J M, Azzi A. Non-antioxidant activities of vitamin E. Current Medicinal Chemistry,2004,11(9): 1113-1133
Zingg J M. Modulation of signal transduction by vitamin E. Molecular Aspects of Medicine,2007, 28(5-6):481-506
Zingg J M. Vitamin E:an overview of major research directions. Molecular Aspects of Medicine,2007, 28(5-6):400-422