全球畜牧养殖业每年产生1010t-1011t的粪便排泄物,其中我国数量为3×109t。这些废弃物中含有大量肠道病原菌,以大肠杆菌、猪链球菌和沙门氏菌数量最多,若未经有效处理就将其排放到土壤或作为有机肥施入到农田,便会严重污染环境并威胁人类健康。近年来,世界各国报道了多起肠道病原菌污染问题,例如2005年我国四川的猪链球菌感染事件以及2011年欧洲的大肠杆菌中毒(毒黄瓜)事件,引起数千人染病或死亡。病原菌进入土壤后,可吸附在土壤颗粒上或随水流进行运移,该过程深刻影响其感染活性和扩散范围。因此,有必要详细研究病原菌与土壤的界面作用和代谢活性机制,以减缓类似事件的爆发,这对于预测病原菌在土壤环境中的分布规律和制定污染土壤修复方案具有重要理论和实际意义。本文以大肠杆菌(Escherichia coli)、猪链球菌(Streptococcus suis)以及多种土壤组分(粘土矿物、地带性和非地带性土壤)为材料,综合运用等温吸附、土柱实验、扫描电子显微镜、微量热、电位滴定、傅里叶变换衰减全反射红外光谱等技术,考察了菌株种类、溶液条件(pH和离子浓度)、土壤类型、有机质含量和胞外聚合物对病原菌吸附的影响,获得了细菌与不同土壤颗粒间的吸附能力、运移距离、表面形貌、代谢活性和官能团变化等信息。同时,借助zeta电位仪、PATH法(Particle adhesion to hydrocarbon)、比表面仪、X射线衍射和土壤物理化学性质测定方法,系统测定了表面电荷、疏水性、比表面积、矿物组成、阳离子交换量、有机质、质地、电导率等性质,用以全面解释互作机制。此外,运用经典的DLVO胶体稳定理论(Classic Derjaguin-Landau-Verwey-Overbeek theory)和统计学手段(一元线性回归、偏相关和多元逐步回归)分析细菌与土壤组分间的作用能信息和显著性影响因素,揭示了吸附过程中的主导作用力及其贡献程度。获得的主要结果如下:
(1)探明了病原菌在粘土矿物表面的吸附行为。二种病原菌在蒙脱石和高岭石上的吸附等温线符合Freundlich方程(R2>0.91),猪链球菌在矿物上吸附的分配系数是大肠杆菌的2倍-8倍,蒙脱石对细菌的吸附量(0.63mL g-1-5.07mLg-1)大于高岭石(0.58mL g-1-1.23mL g-1)。扫描电镜图片显示,大肠杆菌呈杆状,长度大于1μm,猪链球菌粒径略小于大肠杆菌,呈卵圆形。提高溶液pH值(4.0-9.0)或降低离子浓度(20mmol L-1-1mmol L-1)能使细胞和矿物表面负电荷量逐渐增多,静电斥力变大,从而导致吸附量不断减少。DLVO理论计算的作用能数据表明,斥力能障值在该溶液条件下不断升高(1.4快T-408.1kT),与吸附趋势相吻合。各体系吸附量与对应能障值呈显著负相关(pH:Y=-0.031×X+13.4,R2=0.469,P<0.01;离子浓度:Y=-0.004×X+2.7,R2=0.354, P<0.05), DLVO作用力(静电斥力和范德华引力)显著影响着吸附过程。高离子浓度下(20mmol L-1-100mmol L-1),猪链球菌在2种矿物上的吸附量均出现显著下降,偏离了DLVO理论的预测结果,由细菌胞外聚合物与矿物间的空间位阻作用(非DLVO作用力)引起。CaCl2对胶粒表面扩散双电层的压缩作用更强,降低了互作体系间的能障值(0.8kT-52.7kT),且能在细菌和矿物之间形成多价阳离子桥,对吸附量的促进作用强于KCl。
(2)揭示了病原菌在不同粒径土壤颗粒中的吸附、运移与代谢活性规律。土壤颗粒对病原菌吸附能力的大致顺序为:粘粒(<2gm)>粉粒(2μm-48μm)>砂粒(48μm-250μm),去有机质颗粒(9.9×1010cells g-1-59.4×1010cells g-1)>含有机质颗粒(7.8×1010cells g-1-43.9×1010cells g-1)。土壤颗粒表面的zeta电位与比表面积大小与该趋势一致,可从长程的扩散双电层作用力和表面位点角度解释吸附行为,短程疏水作用力和阳离子交换量不能作为土壤-病原菌相互作用的预测指标。微量热参数表明,相比对照体系(只含细菌和培养基),加入粉粒和砂粒后,猪链球菌生长的时间-功率曲线峰值PH相对于自由态细菌(289.6μW)升高了8.1%-27.1%,对应出峰时间PT值(391.0min-408.7min)早于对照体系(424.7min),总体代谢活性增强;粘粒体系PH值下降了11.4%-23.2%,出峰时间较晚(441.7min-464.7min),猪链球菌代谢活性减弱。大肠杆菌在去有机质粘粒上的PH值(119.6μW)小于对照体系(147.2μW),出峰时间更晚(313.5min>281.9min),活性受到抑制,其它5种土壤颗粒均促进大肠杆菌的代谢活性。扫描电镜直接证实了病原菌在砂粒和粉粒表面的吸附,细胞分布较为分散,有利于细胞对土壤颗粒表面吸附的营养物质进行充分分解,促进细菌的代谢活性。粘粒紧密包裹覆盖在细胞上,外表面难以观察到清晰的吸附态细菌,阻碍了细菌生长空间及其与外界营养物质和代谢产物的利用和交换,抑制其生长和代谢过程。猪链球菌能运移到20cm深土层,比大肠杆菌运移距离更远(10cm),物理阻塞对病原菌运移的影响力高于吸附作用。
(3)阐明了病原菌在土壤胶体颗粒上的作用力机制。猪链球菌在红壤胶体表面吸附的分配系数(Kf)是大肠杆菌的4.5倍-6.1倍,细菌在去有机质胶体表面吸附的Kf值为含有机质胶体的2.4倍-3.2倍。比表面积越大或表面负电荷量越少,细菌吸附能力越强,大肠杆菌表面电荷密度较高(541.1μC cm-2),进一步增强与土壤胶体间的静电斥力。猪链球菌和大肠杆菌zeta电位与对应吸附量可分别拟合指数和线性方程,吸附态细菌位于距土壤胶体表面90nm-100nm处的次级小能位置。随着溶液体系pH降低(9.0--4.0)或KCl离子强度增大(1mmol L-1-10mmol L-1),细菌与红壤胶体互作能障降低(354.6kT-0.3kT),次级小能引力增强(-0.020kT--0.536kT),分隔距离不断缩短(111nm-16nm).此时细菌吸附量持续增大,与能障值呈显著负相关(P<0.05),吸附机制符合DLVO理论,疏水作用力影响不显著。高离子强度下(50mmol L-1-100mmol L-1),猪链球菌在去有机质和含有机质颗粒上的吸附量分别降低了3.4%和5.6%,细胞表面蛋白质和土壤有机质共同增强了空间位阻排斥力。
(4)探讨了土壤性质与病原菌吸附能力间的相关性及贡献程度。一元线性回归结果表明,土壤溶液pH(P<0.01)和电导率EC(P=0.033)与猪链球菌分配系数呈显著负相关,能分别解释81.9%和38.4%的吸附过程。大肠杆菌分配系数仅与溶液电导率呈显著正相关(P<0.01),R2值高达0.923。偏相关分析发现,在排除其它因素的间接影响后,pH(P=0.013)和电导率(P=0.034)分别是猪链球菌和大肠杆菌吸附的决定性因素。pH显著促进猪链球菌的吸附量(P<0.05),而电导率此时无显著影响(P>0.05),对吸附能力有极微弱的促进作用(偏相关系数为0.298),与一元线性回归相矛盾。这是因为pH和有机质对吸附能力的抑制作用较大(偏相关系数分别为-0.952和-0.735),从而屏蔽了电导率对土壤的微弱影响,偏相关更能反映单独的某种土壤性质对细菌吸附的真实作用。土壤有机质、粘粒含量、比表面积、阳离子交换量与分配系数无显著相关(P>0.05),仅能单独解释30%以下的吸附行为。扫描电镜手段显示病原菌主要吸附于土壤团聚体的外表面,无法吸附在团聚体内部的小尺寸颗粒表面。球面-平板DLVO理论计算的能障值(Energy barrier, EB)与大肠杆菌分配系数Ks呈显著负相关:Ks=-0.057×EB+22.6(R2=0.577,P<0.01,n=10),但该模型无法解释猪链球菌在土壤颗粒上的吸附行为。通过多元逐步回归手段可得到病原菌分配系数与土壤性质的相关性方程,猪链球菌:Ks=-45.93×pH.1.31×CEC+389.75(R2=0.929,P<0.01);大肠杆菌:Ks=0.24×EC+2.005(R2.=0.932,P<0.01).该方程拟合值与实测值较为接近,相差数值小于14.3mL g-1,可初步用来预测细菌在土壤表面的吸附能力。
(5)明确了胞外聚合物对病原菌表面性质及吸附能力的影响。采用阳离子交换树脂(CER)去除细胞表面的胞外聚合物(Extracellular polymeric substances, EPS),红外光谱数据显示,CER处理后的去EPS细胞在3500cm-1-1000cm-1波数范围内的吸收峰均有明显降低、消失或偏移,表面相应的蛋白质、多糖和脂类物质含量被去除。大肠杆菌细胞壁表面含有羟基、羧基、酰胺基、磷酸基、酯基、醛基、巯基等基团,官能团种类比猪链球菌更为丰富。去除EPS后,2种细菌表面电荷密度和总位点浓度分别降低了7%-17%和3%-7%。离子强度从1mmol L-1上升到100mmolL-1,细菌表面负电荷量逐渐减少。电荷量大小顺序为:含EPS猪链球菌<去EPS猪链球菌<去EPS大肠杆菌<含EPS大肠杆菌。4种细菌表面疏水性范围为3%-43%,去除EPS后,猪链球菌疏水性上升了约5%,大肠杆菌疏水性平均减少了11%。含EPS和去EPS细胞表面性质受官能团种类和EPS组成影响。含EPS猪链球菌在黄棕壤上吸附的分配系数Ks值最大(49.8mL g-1),其次是去EPS猪链球菌(16.1mLg-1)、去EPS大肠杆菌(8.2mL g-1)和含EPS大肠杆菌(8.0mLg-1)。1mmol L-1-60mmol L-1离子强度下的吸附趋势符合球面-平板DLVO模型,吸附量(Y)与斥力能障值(X)呈显著线性负相关:Y=-0.0064×X+2.99(R2=0.602, P<0.01)。当离子强度从60mmol L-1上升到100mmol L-1时,含EPS猪链球菌在土壤颗粒上的吸附量下降了16.0%,而去EPS猪链球菌的吸附量逐渐增大,这表明猪链球菌与土壤间的空间位阻排斥力来源于细胞表面的EPS组分。
The world annually produces1010-1011tons of animal manure, whereas the production is3×109tons in China. These wastes contain large amount of enteric pathogens, the most of which are Escherichia coli, Streptococcus suis and Salmonella enterica. The fecal wastes are partly applied to the soil as a source of plant nutrients. If they are not managed properly, the risk of contaminating environment and threatening human health will be increased severely. In recent years, many countries over the world have reported the problems of enteric pathogenic pollution. For instance, the large outbreaks of Streptococcus suis in China and Escherichia coli in Europe (poisonous cucumber) suddenly appreared in2005and2011, respectively, which caused several thousand people infected or died. After pathogens enter into the soil, they can adsorb on soil particles or be transported with water flow, which remarkably affects their infection activity and the extent of spreading. Therefore, there is a need to comprehensively investigate the mechanisms of interfacial interactions and metabolic activities between pathogens and soils, so as to decrease the exposure of similar incidents. Such knowledge is also of theoretical and practical importance in predicting the pathogen distribution in soil environments and developing plans for the remediation of contaminated soils. The test materials include Escherichia coli, Streptococcus suis and various soil components (clay minerals, zonal and azonal soil types). Adsorption isotherm, soil column experiment, scanning electron microscope, micro-calorimetric, potentiometric titration and attenuated total reflection fourier transform infrared spectroscopy techniques were utilized to study the effects of bacterial strain, solution condition (pH and ionic strength), soil type, organic matter content and extracellular polymeric substances on pathogen adsorption. The information of adsorption capacity, transport distance, surface appearance, metabolic activity and the change of functional groups between bacteria and different soil particles were obtained. Meanwhile, zeta potential analyzer, particle adhesion to hydrocarbon test, specific-surface-area-measuring equipment, X-ray diffraction and soil physicochemical determining methods were employed to systematically evaluate the surface charge, hydrophobicity, specific surface area, mineral constituent, cation exchange capacity, organic matter, soil texture and electric conductivity. These properties were used to explain the overall interaction mechanisms. Additionally, the information of interaction energies and significant impact factors between bacteria and soil components were analyzed by applying the classic Derjaguin-Landau-Verwey-Overbeek theory and statistical tools (simple linear regression, partial correlation and multiple stepwise regression analysis). The major findings were summarized as follows:
(1) Adsorption behaviors of pathogens on clay minerals were investigated. The adsorption isotherms of two pathogenic strains on montmorillonite and kaolinite conformed to the Freundlich equation (R2>0.97). The partition coeffients of S. suis were2-8times as high as those of E. coli. More bacterial cells were found to be adsorbed by montmorillonite (0.63mL g-1-5.07mL g-1) than by kaolinite (0.58mL g-1-1.23mL g-1). Scanning electron microscope images indicate that E. coli is a rod-shaped strain of>1μm-long length, while the size of S. suis is shorter than E. coli and exhibit ovoid shape. Increasing solution pH (4.0-9.0) or decreasing ionic concentrations (20mmol L-1-1mmol L-1) result in the increase of negative charges on bacterial and mineral surfaces, which enlarges the electrostatic repulsions and leads to the reduction of adsorption amount. The interaction energy data calculated by DLVO theory suggest that repulsive energy barriers increased continuously (1.4kT-408.1kT) under these solution conditions, aggreeing with the adsorption trends. Adsorption amounts of each system are significantly negatively correlated with corresponding energy barriers (pH:Y=-0.031×X+13.4, R2=0.469, P<0.01; ionic concentration:Y=-0.004×X+2.7, R2=0.354, P<0.05). DLVO-type forces (electrostatic repulsion and van der Waals attraction) significantly affect the adsorption processes. At higher ionic concentrations (20mmol L-1-100mmol L-1), the adsorption of S. suis on two clay minerals both decreased significantly, which deviated from the results predicted by DLVO theory. This phenomenon was caused by the steric hindrance (non-DLVO force) between the extracellular polymeric substances and clay minerals. CaCl2could efficiently compress the diffuse double layer outside the colloidal surfaces, decrease the energy barriers (0.8kT-52.7kT) among the interaction systems, and form multivalent-cation-bridge between bacteria and minerals. These effects induced the result that CaCl2was more effective than KCl in enhancing adsorption amount.
(2) The adsorption, transport and metabolic activity phenomena of pathogens on soil particles of different sizes were elucidated. The adsorption capacities of pathogens on soil particles generally followed the order:clay (<2μm)> silt (2μm-48μm)> sand (48μm-250μm), inorganic particle (9.9×1010cells g-1-59.4×1010cells g-1)> organic particle (7.8×1010cells g-1-43.9×1010cells g-1). The zeta potential and specific surface area values of soil particles were consistent with the adsorption trends. The long-range diffuse double layer interaction force and surface available site contributed to the adsorption behaviors. However, the short-range hydrophobic force and cation exchange capacity values could not be considered as the parameters for predicting the soil-pathogen interactions. Micro-calorimetric data showed that the PH values of power-time curves in the systems of silts and sands increased by8.1%-27.1%than those in the system of free S. suis (289.6μW). The corresponding PT values occurred earlier (391.0min-408.7min) than the control experiment (424.7min). Metabolic activities of S. suis were enhanced. As a result of the decreasing PH (11.4%-23.2%) and larger PT values (441.7min-464.7min), the metabolic activities of the S. suis-clay systems were inhibited. The PH value of E. coli-inorganic clay system (119.6μW) was lower than that of control experiment (147.2μW), and its PT value occurred later (313.5min>281.9min), which restrained the acivity of E. coli. The other five soil particle types all promoted E. coli's metabolic activities. Scanning electron microscope directly confirmed that pathogens adsorbed on silt and sand surfaces, and the cells were dispersedly distributed. Thus, the adsorbed nutrient substances could help the cells to decompose nutrients more sufficiently, which promoted bacterial metabolic activities. The cell surfaces were covered with clays tightly, and the soil outside surfaces did not show adsorbed cells distinctly. This phenomenon restrained the bacterial utilization and exchange processes of external nutrient materials and metabolites, as well as their growth spaces. So the clay particles repressed bacterial growth and metabolic activities.5. suis was able to transport to the20cm-depth soil layer, which was deeper than E. coli (10cm-depth). Physical straining had greater influence on pathogen transport than adsorption behavior.
(3) The interaction force mechanisms of pathogen adsorption on soil colloidal particles were clarified. The partition coefficients (Kf) of S. suis adsorption on soil colloids were4.5-6.4times as large as those of E. coli, while the Kf values of bacterial adsorption on inorganic colloids were2.4-3.2times as large as those on organic colloids. The larger the specific surface areas and the fewer negative charges on cell or soil colloidal surface, the greater adsorption capacity of bacteria. The surface charge density of E. coli was higher, further strengthening the electrostatic repulsions between E. coli cells and soil colloids. The correlations between the zeta potentials of S. suis and E. coli and the corresponding adsorption amount fitted exponential and linear equations, respectively. Pathogens adsorbed in the secondary energy minima at separation distances of90nm-100nm away from the soil colloids. With the decrease of pH (9.0-4.0) or the increase of KCl concentrations (1mmol L-1-10mmol L-1), the interaction energy barriers between the cells and soil colloids reduced (354.6kT-0.3kT). The attractive secondary energy minima increased (-0.020kT--0.536kT), and the separation distances decreased (111nm-16nm) constantly. Under these conditions, bacterial adsorption amount increased continuously, which were significantly negatively correlated with the energy barriers (P<0.05) and consistent with DLVO theory. Hydrophobic force had a negligible impact on cell adsorption. At higher ionic strengths (50mmol L-1-100mmol L"1), S. suis adsorption on inorganic and organic colloids decreased by3.4%and5.6%, respectively. Cell surface proteins and soil organic matter both enchanced the steric repulsions.
(4) The correlations and contribution rates between soil properties and pathogen adsorption capacities were analyzed. Simple linear regression results show that soil solution pH (P<0.01) and electric conductivity (P=0.033) were significantly negatively correlated with the partition coefficients of S. suis, which could explain81.9%and38.4%of the adsorption processes. The partition coefficients of E. coli were only significantly correlated (positive) with solution electric conductivity, with R2values of0.923. Partial correlation analysis found that after excluding the indirect effects of other factors, pH (P=0.013) and electric conductivity{P=0.034) were the determinant factors of S. suis and E. coli adsorption, respectively. Solution pH increased S. suis adsorption significantly (P <0.05), while electric conductivity did not show a significant effect-(P>0.05) and had a little positive influence (partial correlation coefficient0.298). These data did not agree with the simple linear regression analysis. Because pH (partial correlation coefficient-0.952) and organic matter (partial correlation coefficient-0.735) had great suppressive impacts on the adsorption capacities, they could mask the weak effect of electric conductivity. Thereby, partial correlation was able to reflect the real influence of a certain soil property on bacterial adsorption. Soil organic matter, clay content, specific surface area and cation exchange capacity had insignificant impacts on partition coefficients (P>0.05), which could only explain less than30%of the adsorption behavior. Scanning electron microscope technique suggests that pathogens mainly adsorbed on the external surfaces of soil aggregates. Bacteria could not adsorb on the internal small-size particle surfaces of aggregates. The partition coefficients of E. coli were significantly negatively correlated with the corresponding energy barriers calculated by sphere-plate DLVO theory:Ks=-0.057×EB+22.6(R2=0.577, P<0.01, n=10). However, this model could not explain the adsorption behaviors of S. suis on soil particles. Correlation equations between pathogen partition coefficients and soil properties were obtained by applying multiple stepwise regression method. The equations were shown below:S. suis-Ks=-45.93×pH-1.31×CEC+389.75(R2=0.929, P<0.01); E. coli-Ks=0.24×EC+2.005(R2-0.932, P<0.01). The partition coefficients calculated by the two equations were comparable with the measured values, with discrepant values less than14.3mL g-1. These models were able to initially predict the adsorption capacities of pathogens on soil surfaces.
(5) The effects of extracellular polymeric substances (EPS) on pathogenic suface properties and adsorption capacities were interpreted. Cation exchange resin (CER) was employed to remove the EPS on cell surface. Infrared spectrum data show that the absorption peaks of EPS-removed cells between3500cm-1and1000cm-1reduced, disappeared or deviated after the treatment of CER, indicating the corresponding amounts of proteins, polysaccharides and lipid materials were removed. The cell wall surface of E. coli contains hydroxyl, carboxyl, amido, phosphate, ester, aldehyde and sulphydryl groups, which was more plentiful than the functional group types of S. suis. After the removal of EPS, the surface charge densities and total site concentrations of two bacterial strains reduced by7%-17%and3%-7%, respectively. When the ionic strength increased from1mmol L-1to100mmol L"1, the negative charges on bacterial surface decreased constantly, following the order of EPS-left S. suis
引文
1.鲍士旦.土壤农化分析.北京:中国农业出版社,2000
2.陈欣,常志州,袁生,叶小梅,费辉盈,朱红.畜禽粪便中人畜共患病原菌对蔬菜污染的研究.江苏农业科学,2007,5:238-239
3.方临川.重金属在细菌-土壤活性颗粒微界面互作的分子机制.[博士学位论文].武汉:华中农业大学图书馆,2011
4.黄巧云.土壤学.北京:中国农业出版社,2006
5.洪志能.枯草芽孢杆菌与土壤矿物界面作用机理.[博士学位论文].武汉:华中农业大学图书馆,2012
6.蒋代华.细菌在粘土矿物及土壤颗粒表面的吸附研究.[博士学位论文].武汉:华中农业大学图书馆,2009
7. 李桂花,李保国.大肠杆菌在饱和砂土中的运移及其模拟.土壤学报,2003,40:783-786
8.李国学.固体废物处理与资源化.北京:中国环境科学出版社,2005
9.李庆康,张永春,杨其飞,杨卓亚,李延.生物有机肥肥效机理及应用前景展望.中国生态农业学报,2003,11:78-80
10.刘梦元,吴斌,刘建杰,彭贵青,刘国平,陈焕春.规模化猪场大肠杆菌的耐药性监测及血清流行病学调查.中国兽医学报,2004,24:16-18
11.罗淑琴.安全猪肉在养殖环节存在的问题及对策.畜禽业,2006,1:54-55
12.马怀良,许修宏.畜禽粪便高温堆肥化处理技术.东北农业大学学报,2005,36:536-540
13.秦耀东.土壤物理学.北京:高等教育出版社,2003
14.荣兴民.几种细菌与土壤粘粒矿物相互作用的热力学研究.[博士学位论文].武汉:华中农业大学图书馆,2008
15.荣兴民,黄巧云,陈雯莉,梁巍.土壤矿物与微生物相互作用的机理及其环境效应.生态学报,2008,28:376-387
16.史素云.大肠杆菌毒力因子研究进展.现代农业科技,2008:271-272
17.翁诗甫.傅里叶变换红外光谱分析.北京:化学工业出版社,2010
18.吴立志,王金良,肖跃强,李书光,祖立闯,伍晓雄,沈志强.猪链球菌2型毒力因子研究进展.动物医学进展,2012,33:118-122
19.熊毅.土壤胶体研究方法.北京:科学出版社,1985
20.杨敏,豆小敏,张昱.固液界面吸附机制与模型.环境科学学报,2006,26:1581-1585
21.曾希柏,杨正礼.中国农业环境质量状况与保护对策.应用生态学报,2006,17:131-136
22.张健.畜禽粪便源环境风险物质在土壤中的变化特征研究.[博士学位论文].沈阳:沈阳农业大学图书馆,2011
23.张璐晶.疯狂的欧洲“疫病”补偿2.1亿欧元难修复欧盟各国裂痕.中国经济周刊,2011:66-67
24.张作祥,蔡星,张铭.AUTOSORB-1表面和孔径分布测定仪及其在石化方面的应用.第九届全国粉体工程学术会暨相关设备,产品交流会论文专辑,沈阳,2003,北京:中国非金属矿工业导刊,2003,55-57
25.郑华英,周敦金,李连春,饶定茂,朱焰,夏虹,夏江,孙敏.武汉地区粪便经沼气发酵后用于绿色蔬菜种植和养鱼卫生效果评价.中国卫生检验杂志,2004,13:730-733
26. Abu-Ashour J, Joy DM, Lee H, Whiteley HR, Zelin S. Transport of microorganisms through soil. Water, Air,& Soil Pollution,1994,75:141-158
27. Abu-Lail NI, Camesano TA. Role of ionic strength on the relationship of biopolymer conformation, DLVO contributions, and steric interactions to bioadhesion of Pseudomonasputida KT2442. Biomacromolecules,2003,4:1000-1012
28. Alizadeh-Pasdar N, Li-Chan EC. Comparison of protein surface hydrophobicity measured at various pH values using three different fluorescent probes. Journal of Agricultural and Food Chemistry,2000,48:328-334
29. Amirbahman A, Olson TM. Transport of humic matter-coated hematite in packed beds. Environmental Science & Technology,1993,27:2807-2813
30. Ams DA, Fein JB, Dong H, Maurice PA. Experimental measurements of the adsorption of Bacillus subtilis and Pseudomonas mendocina onto Fe-oxyhydroxide-coated and uncoated quartz grains. Geomicrobiology Journal,2004, 21:511-519
31. An YH, Friedman RJ. Concise review of mechanisms of bacterial adhesion to biomaterial surfaces. Journal of Biomedical Materials Research,1998,43:338-348
32. Arthurson V, Sessitsch A, Jaderlund L. Persistence and spread of Salmonella enterica serovar Weltevreden in soil and on spinach plants. FEMS Microbiology Letters,2011, 314:67-74
33. Barros N, Feijoo S, Simoni JA, Prado AG, Barboza FD, Airoldi C. Microcalorimetric study of some Amazonian soils. Thermochimica Acta,1999,328:99-103
34. Becker MW, Collins SA, Metge DW, Harvey RW, Shapiro AM. Effect of cell physicochemical characteristics and motility on bacterial transport in groundwater. Journal of Contaminant Hydrology,2004,69:195-213
35. Bengtsson G. Growth and metabolic flexibility in groundwater bacteria. Microbial Ecology,1989,18:235-248
36. Bob MM, Walker HW. Effect of natural organic coatings on the polymer-induced coagulation of colloidal particles. Colloids and Surfaces A:Physicochemical and Engineering Aspects,2000,177:215-222
37. Bolster C, Haznedaroglu B, Walker S. Diversity in cell properties and transport behavior among 12 different environmental isolates. Journal of Environmental Quality,2009,38:465-472
38. Bolster CH, Cook KL, Marcus IM, Haznedaroglu BZ, Walker SL. Correlating transport behavior with cell properties for eight porcine Escherichia coli isolates. Environmental Science & Technology,2010,44:5008-5014
39. Bolster CH, Mills AL, Hornberger GM, Herman JS. Effect of surface coatings, grain size, and ionic strength on the maximum attainable coverage of bacteria on sand surfaces. Journal of Contaminant Hydrology,2001,50:287-305
40. Borrok D, Fein JB. Distribution of protons and Cd between bacterial surfaces and dissolved humic substances determined through chemical equilibrium modeling. Geochimica et Cosmochimica Acta,2004,68:3043-3052
41. Bos R, Mei HC, Busscher HJ. Physico-chemistry of initial microbial adhesive interactions-its mechanisms and methods for study. FEMS Microbiology Reviews, 1999,23:179-230
42. Bradford SA, Bettahar M, Simunek J, Van Genuchten MT. Straining and attachment of colloids in physically heterogeneous porous media. Vadose Zone Journal,2004,3: 384-394
43. Bradford SA, Simunek J, Bettahar M, Tadassa YF, van Genuchten MT, Yates SR. Straining of colloids at textural interfaces. Water Resources Research,2005,41, W10404, doi:10.1029/2004WR003675
44. Bradford SA, Simunek J, Bettahar M, van Genuchten MT, Yates SR. Modeling colloid attachment, straining, and exclusion in saturated porous media. Environmental Science & Technology,2003,37:2242-2250
45. Bradford SA, Simunek J, Walker SL. Transport and straining of E. coli O157:H7 in saturated porous media. Water Resources Research,2006a,42, W12S12, DOI: 10.1029/2005WR004805
46. Bradford SA, Tadassa YF, Pachepsky Y. Transport of and manure suspensions in saturated porous media. Journal of Environmental Quality,2006b,35:749-757
47. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry,1976,72:248-254
48. Brady NC, Weil RR. The nature and properties of soils. Upper Saddle River: Prentice-Hall Inc,1996
49. Brown DG, Stencel JR, Jaffe PR. Effects of porous media preparation on bacteria transport through laboratory columns. Water Research,2002,36:105-114
50. Caccavo F, Schamberger PC, Keiding K, Nielsen PH. Role of hydrophobicity in adhesion of the dissimilatory Fe(III)-reducing bacterium Shewanella alga to amorphous Fe(III) oxide. Applied and Environmental Microbiology,1997,63: 3837-3843
51. Cai P, Huang Q, Zhang X. Interactions of DNA with clay minerals and soil colloidal particles and protection against degradation by DNase. Environmental Science & Technology,2006a,40:2971-2976
52. Cai P, Huang Q, Walker SL. Deposition and survival of Escherichia coli 0157:H7 on clay minerals in a parallel plate flow system. Environmental Science & Technology, 2013,47:1896-1903
53. Cai P, Huang Q, Zhang X. Microcalorimetric studies of the effects of MgCl2 concentrations and pH on the adsorption of DNA on montmorillonite, kaolinite and goethite. Applied Clay Science,2006b,32:147-152
54. Camesano TA, Logan BE. Probing bacterial electrosteric interactions using atomic force microscopy. Environmental Science & Technology,2000,34:3354-3362
55. Cao Y, Wei X, Cai P, Huang Q, Rong X, Liang W. Preferential adsorption of extracellular polymeric substances from bacteria on clay minerals and iron oxide. Colloids and Surfaces B:Biointerfaces,2011,83:122-127
56. Castro FD, Tufenkji N. Relevance of nontoxigenic strains as surrogates for Escherichia coli O157:H7 in groundwater contamination potential:role of temperature and cell acclimation time. Environmental Science & Technology,2007, 41:4332-4338
57. Chen G, Rockhold M, Strevett KA. Equilibrium and kinetic adsorption of bacteria on alluvial sand and surface thermodynamic interpretation. Research in Microbiology, 2003,154:175-181
58. Chen G, Walker SL. Role of solution chemistry and ion valence on the adhesion kinetics of groundwater and marine bacteria. Langmuir,2007,23:7162-7169
59. Chen G, Walker SL. Fecal indicator bacteria transport and deposition in saturated and unsaturated porous media. Environmental Science & Technology,2012,46: 8782-8790
60. Chen G, Zhu H. Bacterial adhesion to silica sand as related to Gibbs energy variations. Colloids and Surfaces B:Biointerfaces,2005,44:41-48
61. Chenu C, Stotzky G, Huang P, Bollag J, Senesi N. Interactions between microorganisms and soil particles:an overview. In:Huang PM, Bollag JM, Senesi N eds., Interactions Between Soil Particles and Microorganisms:Impact on the Terrestrial Ecosystem. Chichester:John Wiley & Sons Ltd,2001.3-40
62. Chu Y, Jin Y, Baumann T, Yates MV. Effect of soil properties on saturated and unsaturated virus transport through columns. Journal of Environmental Quality,2003, 32:2017-2025
63. Cizek AR, Characklis GW, Krometis LA, Hayes JA, Simmons OD, Lonardo SD, Alderisio KA, Sobsey MD. Comparing the partitioning behavior of Giardia and Cryptosporidium with that of indicator organisms in stormwater runoff. Water Research,2008,42:4421-4438
64. Danese PN, Pratt LA, Kolter R. Exopolysaccharide production is required for development of Escherichia coli K-12 biofilm architecture. Journal of Bacteriology, 2000,182:3593-3596
65. Deo N, Natarajan K, Somasundaran P. Mechanisms of adhesion of Paenibacillus polymyxa onto hematite, corundum and quartz. International Journal of Mineral Processing,2001,62:27-39
66. Dhand NK, Toribio JA, Whittington RJ. Adsorption of Mycobacterium avium subsp. paratuberculosis to soil particles. Applied and Environmental Microbiology,2009,75: 5581-5585
67. Doran JW, Linn D. Bacteriological quality of runoff water from pastureland. Applied and Environmental Microbiology,1979,37:985-991
68. DuPont HL. Travelers'diarrhea:antimicrobial therapy and chemoprevention. Nature Clinical Practice Gastroenterology & Hepatology,2005,2:191-198
69. Egli T, Weilenmann HU, El-Banna T, Auling G. Gram-negative, aerobic, nitrilotriacetate-utilizing bacteria from wastewater and soil. Systematic and Applied Microbiology,1988,10:297-305
70. Eichler M, Dahlhaus R, Sandkuhler J. Partial correlation analysis for the identification of synaptic connections. Biological Cybernetics,2003,89:289-302
71. Elimelech M, Nagai M, Ko CH, Ryan JN. Relative insignificance of mineral grain zeta potential to colloid transport in geochemically heterogeneous porous media. Environmental Science & Technology,2000,34:2143-2148
72. Emerson RJ, Camesano TA. Nanoscale investigation of pathogenic microbial adhesion to a biomaterial. Applied and Environmental Microbiology,2004,70: 6012-6022
73. Etter MC. Hydrogen bonds as design elements in organic chemistry. The Journal of Physical Chemistry,1991,95:4601-4610
74. Fang L, Wei X, Cai P, Huang Q, Chen H, Liang W, Rong X. Role of extracellular polymeric substances in Cu(Ⅱ) adsorption on Bacillus subtilis and Pseudomonas putida. Bioresource Technology,2011,102:1137-1141
75. Farahat M, Hirajima T, Sasaki K, Aiba Y, Doi K. Adsorption of SIP E. coli onto quartz and its applications in froth flotation. Minerals Engineering,2008,21:389-395
76. Fayer R, Trout JM. Zoonotic protists in the marine environment. In:Belkin S, Colwell RR eds., Oceans and Health:Pathogens in the Marine Environment. Berlin: Springer US,2005.143-163
77. Fein JB, Boily JF, Yee N, Gorman-Lewis D, Turner BF. Potentiometric titrations of Bacillus subtilis cells to low pH and a comparison of modeling approaches. Geochimica et Cosmochimica Acta,2005,69:1123-1132
78. Fletcher M. The effects of culture concentration and age, time, and temperature on bacterial attachment to polystyrene. Canadian Journal of Microbiology,1977,23:1-6
79. Fletcher M, Loeb G Influence of substratum characteristics on the attachment of a marine pseudomonad to solid surfaces. Applied and Environmental Microbiology, 1979,37:67-72
80. Foppen JW, Liem Y, Schijven J. Effect of humic acid on the attachment of Escherichia coli in columns of goethite-coated sand. Water Research,2008,42: 211-219
81. Franz E, Semenov AV, Termorshuizen AJ, De Vos 0, Bokhorst JG, Van Bruggen AH. Manure-amended soil characteristics affecting the survival of E. coli O157:H7 in 36 Dutch soils. Environmental Microbiology,2008,10:313-327
82. Franz E, Van Diepeningen AD, De Vos OJ, Van Bruggen AH. Effects of cattle feeding regimen and soil management type on the fate of Escherichia coli O157:H7 and Salmonella enterica serovar typhimurium in manure, manure-amended soil, and lettuce. Applied and Environmental Microbiology,2005,71:6165-6174
83. Gallardo-Moreno AM, Gonzalez-Martin ML, Bruque JM, Perez-Giraldo C, Gomez-Garcia AC. Temperature influence on the physicochemical surface properties and adhesion behaviour of Enterococcus faecalis to glass and silicone. Journal of Adhesion Science and Technology,2002,16:1215-1223
84. Gannon J, Manilal V, Alexander M. Relationship between cell surface properties and transport of bacteria through soil. Applied and Environmental Microbiology,1991,57: 190-193
85. Gao X, Chorover J. In-situ monitoring of Cryptosporidium parvum oocyst surface adhesion using ATR-FTIR spectroscopy. Colloids and Surfaces B:Biointerfaces, 2009,71:169-176
86. Gargiulo G, Bradford S, Simunek J, Ustohal P, Vereecken H, Klumpp E. Bacteria transport and deposition under unsaturated conditions:the role of the matrix grain size and the bacteria surface protein. Journal of Contaminant Hydrology,2007,92: 255-273
87. Garzio-Hadzick A, Shelton DR, Hill RL, Pachepsky YA, Guber AK, Rowland R. Survival of manure-borne E. coli in streambed sediment:effects of temperature and sediment properties. Water Research,2010,44:2753-2762
88. Gordesli FP, Abu-Lail NI. The role of growth temperature in the adhesion and mechanics of pathogenic L. monocytogenes:an AFM Study. Langmuir,2011,28: 1360-1373
89. Gordesli FP, Abu-Lail NI. Combined poisson and soft-particle DLVO analysis of the specific and nonspecific adhesion forces measured between L. monocytogenes grown at various temperatures and silicon nitride. Environmental Science & Technology, 2012,46:10089-10098
90. Gottschalk M, Segura M, Xu J. Streptococcus suis infections in humans:the Chinese experience and the situation in North America. Animal Health Research Reviews, 2007,8:29-46
91. Grasso D, Subramaniam K, Butkus M, Strevett K, Bergendahl J. A review of non-DLVO interactions in environmental colloidal systems. Reviews in Environmental Science and Biotechnology,2002,1:17-38
92. Gregory J. Approximate expressions for retarded van der Waals interaction. Journal of Colloid Interface Science,1981,83:138-145
93. Guber AK, Pachepsky YA, Shelton DR, Yu O. Effect of bovine manure on fecal coliform attachment to soil and soil particles of different sizes. Applied and Environmental Microbiology,2007,73:3363-3370
94. Guber AK, Shelton DR, Pachepsky YA. Effect of manure on Escherichia coli attachment to soil. Journal of Environmental Quality,2005,34:2086-2090
95. Hahn MW, O'Melia CR. Deposition and reentrainment of Brownian particles in porous media under unfavorable chemical conditions:some concepts and applications. Environmental Science & Technology,2004,38:210-220
96. Hamadi F, Latrache H, Zahir H, Elghmari A, Timinouni M, Ellouali M. The relation between Escherichia coli surface functional groups' composition and their physicochemical properties. Brazilian Journal of Microbiology,2008,39:10-15
97. Harden VP, Harris JO. The isoelectric point of bacterial cells. Journal of Bacteriology, 1953,65:198-202
98. Harvey RW. Parameters involved in modeling movement of bacteria in groundwater. In:Hurst CJ ed., Modeling the Environmental Fate of Microorganisms. Washington, DC:ASM Press,1991.89-114
99. Haznedaroglu B, Bolster C, Walker S. The role of starvation on Escherichia coli adhesion and transport in saturated porous media. Water Research,2008,42: 1547-1554
100.Haznedaroglu B, Kim H, Bradford S, Walker S. Relative transport behavior of Escherichia coli O157:H7 and Salmonella enterica serovar pullorum in packed bed column systems:influence of solution chemistry and cell concentration. Environmental Science & Technology,2009,43:1838-1844
101.He LM, Tebo BM. Surface charge properties of and Cu(II) adsorption by spores of the marine Bacillus sp. strain SG-1. Applied and Environmental Microbiology,1998, 64:1123-1129
102.He Y, Xu J, Wang H, Ma Z, Chen J. Detailed sorption isotherms of pentachlorophenol on soils and its correlation with soil properties. Environmental Research,2006,101: 362-372
103.Hermansson M. The DLVO theory in microbial adhesion. Colloids and Surfaces B: Biointerfaces,1999,14:105-119
104.Herzberg M, Rezene TZ, Ziemba C, Gillor O, Mathee K. Impact of higher alginate expression on deposition of Pseudomonas aeruginosa in radial stagnation point flow and reverse osmosis systems. Environmental Science & Technology,2009,43: 7376-7383
105.Hiemenz P, Rajagopalan R. Electrophoresis and other electrokinetic phenomena. Principles of Colloid and Surface Chemistry. New York:Dekker,1977.452-487
106.Hogg R, Healy T, Fuerstenau D. Mutual coagulation of colloidal dispersions. Transactions of the Faraday Society,1966,62:1638-1651
107.Holm PE, Nielsen PH, Albrechtsen HJ, Christensen TH. Importance of unattached bacteria and bacteria attached to sediment in determining potentials for degradation of xenobiotic organic contaminants in an aerobic aquifer. Applied and Environmental Microbiology,1992,58:3020-3026
108.Hong Z, Rong X, Cai P, Dai K, Liang W, Chen W, Huang Q. Initial adhesion of Bacillus subtilis on soil minerals as related to their surface properties. European Journal of Soil Science,2012,63:457-466
109.Hori K, Matsumoto S. Bacterial adhesion:from mechanism to control. Biochemical Engineering Journal,2010,48:424-434
110.Hrudey S, Payment P, Huck P, Gillham R, Hrudey E. A fatal waterborne disease epidemic in Walkerton, Ontario:comparison with other waterborne outbreaks in the developed world. Water Science & Technology,2003,47:7-14
111.Huang P. Soil mineral-organic matter-microorganism interactions:fundamentals and impacts. Advances in Agronomy,2004,82:391-472
112.Ibekwe AM, Papiernik SK, Grieve CM, Yang CH. Quantification of persistence of Escherichia coli O157:H7 in contrasting soils. International Journal of Microbiology, 2011, doi:10.1155/2011/421379
113.Islam M, Doyle MP, Phatak SC, Millner P, Jiang X. Persistence of enterohemorrhagic Escherichia coli O157:H7 in soil and on leaf lettuce and parsley grown in fields treated with contaminated manure composts or irrigation water. Journal of Food Protection,2004,67:1365-1370
114.Israelachvili J, Pashley R. Measurement of the hydrophobic interaction between two hydrophobic surfaces in aqueous electrolyte solutions. Journal of Colloid Interface Science,1984,98:500-514
115.Jacobs A, Lafolie F, Herry J, Debroux M. Kinetic adhesion of bacterial cells to sand: cell surface properties and adhesion rate. Colloids and Surfaces B:Biointerfaces, 2007,59:35-45
116.Jacobsen OH, Moldrup P, Larsen C, Konnerup L, Petersen LW. Particle transport in macropores of undisturbed soil columns. Journal of Hydrology,1997,196:185-203
117.Jawson M, Elliott L, Saxton K, Fortier D. The effect of cattle grazing on indicator bacteria in runoff from a Pacific Northwest watershed. Journal of Environmental Quality,1982,11:621-627
118.Jeng HC, Andrew J, Bradford HB. Indicator organisms associated with stormwater suspended particles and estuarine sediment. Journal of Environmental Science and Health,2005,40:779-791
119.Jewett DG, Hilbert TA, Logan BE, Arnold RG, Bales RC. Bacterial transport in laboratory columns and filters:influence of ionic strength and pH on collision efficiency. Water Research,1995,29:1673-1680
120.Jiang D, Huang Q, Cai P, Rong X, Chen W. Adsorption of Pseudomonas putida on clay minerals and iron oxide. Colloids and Surfaces B:Biointerfaces,2007,54: 217-221
121.Jiang W, Saxena A, Song B, Ward BB, Beveridge TJ, Myneni SC. Elucidation of functional groups on gram-positive and gram-negative bacterial surfaces using infrared spectroscopy. Langmuir,2004,20:11433-11442
122.Johnson WP, Martin MJ, Gross MJ, Logan BE. Facilitation of bacterial transport through porous media by changes in solution and surface properties. Colloids and Surfaces A:Physicochemical and Engineering Aspects,1996,107:263-271
123.Jun Y, Yi L, Yong T, Jianben L, Xiong C, Qin Z, Jiaxin D, Songsheng Q, Ziniu Y. Action of Cu2+ on Bacillus thuringiensis growth investigated by microcalorimetry. Applied Biochemistry and Microbiology,2003,39:576-580
124.Kay R. Streptococcus suis, Streptococcus mitis, what is the problem? Hong Kong. Medical Journal,2005,11:321
125.Keller AA, Auset M. A review of visualization techniques of biocolloid transport processes at the pore scale under saturated and unsaturated conditions. Advances in Water Resources,2007,30:1392-1407
126.Khemakhem W, Ammar E, Bakhrouf A. Effect of environmental conditions on hydrophobicity of marine bacteria adapted to textile effluent treatment. World Journal of Microbiology and Biotechnology,2005,21:1623-1631
127.Kim HN, Hong Y, Lee I, Bradford SA, Walker SL. Surface characteristics and adhesion behavior of Escherichia coli O157:H7:role of extracellular macromolecules. Biomacromolecules,2009,10:2556-2564
128.Kim HN, Walker SL. Escherichia coli transport in porous media:influence of cell strain, solution chemistry, and temperature. Colloids and Surfaces B:Biointerfaces, 2009,71:160-167
129.Kim HN, Walker SL, Bradford SA. Macromolecule mediated transport and retention of Escherichia coli O157:H7 in saturated porous media. Water Research,2010,44: 1082-1093
130.Kim SB, Park SJ, Lee CG, Choi NC, Kim DJ. Bacteria transport through goethite-coated sand:effects of solution pH and coated sand content. Colloids and Surfaces B:Biointerfaces,2008,63:236-242
131.Kinoshita T, Bales RC, Yahya MT, Gerba CP. Bacteria transport in a porous medium: retention of Bacillus and Pseudomonas on silica surfaces. Water Research,1993,27: 1295-1301
132.Kinzler K, Gehrke T, Telegdi J, Sand W. Bioleaching-a result of interfacial processes caused by extracellular polymeric substances (EPS). Hydrometallurgy,2003,71: 83-88
133.Knapp EP, Herman JS, Hornberger GM, Mills AL. The effect of distribution of iron-oxyhydroxide grain coatings on the transport of bacterial cells in porous media. Environmental Geology,1998,33:243-248
134.Kuznar ZA, Elimelech M. Adhesion kinetics of viable Cryptosporidium parvum oocysts to quartz surfaces. Environmental Science & Technology,2004,38: 6839-6845
135.Lavie S, Stotzky G. Adhesion of the clay minerals montmorillonite, kaolinite, and attapulgite reduces respiration of Histoplasma capsulatum. Applied and Environmental Microbiology,1986,51:65-73
136.Li B, Logan BE. Bacterial adhesion to glass and metal-oxide surfaces. Colloids and Surfaces B:Biointerfaces,2004,36:81-90
137.Li J, McLandsborough L. The effects of the surface charge and hydrophobicity of Escherichia coli on its adhesion to beef muscle. International Journal of Food Microbiology,1999,53:185-193
138.Li Q, Elimelech M. Organic fouling and chemical cleaning of nanofiltration membranes:measurements and mechanisms. Environmental Science & Technology, 2004,38:4683-4693
139.Li Q, Logan BE. Enhancing bacterial transport for bioaugmentation of aquifers using low ionic strength solutions and surfactants. Water Research,1999,33:1090-1100
140.Liu Y, Yang CH, Li J. Influence of extracellular polymeric substances on Pseudomonas aeruginosa transport and deposition profiles in porous media. Environmental Science & Technology,2007,41:198-205
141.Long G, Zhu P, Shen Y, Tong M. Influence of extracellular polymeric substances (EPS) on deposition kinetics of bacteria. Environmental Science & Technology,2009, 43:2308-2314
142.Mafu AA, Roy D, Goulet J, Magny P. Attachment of Listeria monocytogenes to stainless steel, glass, polypropylene, and rubber surfaces after short contact times. Journal of Food Protection,1990,53:742-746
143.Marshall K, Stout R, Mitchell R. Mechanism of the initial events in the sorption of marine bacteria to surfaces. Journal of General Microbiology,1971,68:337-348
144.Mawdsley JL, Bardgett RD, Merry RJ, Pain BF, Theodorou MK. Pathogens in livestock waste, their potential for movement through soil and environmental pollution. Applied Soil Ecology,1995,2:1-15
145.Mayer C, Moritz R, Kirschner C, Borchard W, Maibaum R, Wingender J, Flemming HC. The role of intermolecular interactions:studies on model systems for bacterial biofilms. International Journal of Biological Macromolecules,1999,26:3-16
146.Mceldowney S, Fletcher M. Effect of growth conditions and surface characteristics of aquatic bacteria on their attachment to solid surfaces. Journal of General Microbiology,1986,132:513-523
147.McEldowney S, Fletcher M. Effect of pH, temperature, and growth conditions on the adhesion of a gliding bacterium and three nongliding bacteria to polystyrene. Microbial Ecology,1988,16:183-195
148.Mead PS, Slutsker L, Dietz V, McCaig LF, Bresee JS, Shapiro C, Griffin PM, Tauxe RV. Food-related illness and death in the United States. Emerging Infectious Diseases, 1999,5:607-625
149.Meyer EE, Rosenberg KJ, Israelachvili J. Recent progress in understanding hydrophobic interactions. Proceedings of the National Academy of Sciences,2006, 103:15739-15746
150.Mills AL, Herman JS, Hornberger GM, DeJesus TH. Effect of solution ionic strength and iron coatings on mineral grains on the sorption of bacterial cells to quartz sand. Applied and Environmental Microbiology,1994,60:3300-3306
151.Morrow J, Stratton R, Yang HH, Smets BF, Grasso D. Macro-and nanoscale observations of adhesive behavior for several E. coli strains (O157:H7 and environmental isolates) on mineral surfaces. Environmental Science & Technology, 2005,39:6395-6404
152.Mosley LM, Hunter KA, Ducker WA. Forces between colloid particles in natural waters. Environmental Science & Technology,2003,37:3303-3308
153.Muirhead RW, Collins RP, Bremer PJ. Interaction of Escherichia coli and soil particles in runoff. Applied and Environmental Microbiology,2006,72:3406-3411
154.Myers A, Prausnitz JM. Thermodynamics of mixed-gas adsorption. AIChE Journal, 2004,11:121-127
155.Nunez-Regueira L, Rodriguez-Anon JA, Proupin-Castineiras J, Nunez-Fernandez O. Microcalorimetric study of changes in the microbial activity in a humic Cambisol after reforestation with eucalyptus in Galicia (NW Spain). Soil Biology and Biochemistry,2006,38:115-124
156.Nannipieri P, Ascher J, Ceccherini M, Landi L, Pietramellara G, Renella G. Microbial diversity and soil functions. European Journal of Soil Science,2003,54:655-670
157.Nikaido H. Molecular basis of bacterial outer membrane permeability revisited. Microbiology and Molecular Biology Reviews,2003,67:593-656
158.Nodar R, Acea M, Carballas T. Poultry slurry microbial population:composition and evolution during storage. Bioresource Technology,1992,40:29-34
159.Ojeda J, Romero-Gonzalez M, Pouran H, Banwart S. In situ monitoring of the biofilm formation of Pseudomonas putida on hematite using flow-cell ATR-FTIR spectroscopy to investigate the formation of inner-sphere bonds between the bacteria and the mineral. Mineralogical Magazine,2008,72:101-106
160.Oliver DM, Clegg CD, Heathwaite AL, Haygarth PM. Preferential attachment of Escherichia coli to different particle size fractions of an agricultural grassland soil. Water, Air,& Soil Pollution,2007,185:369-375
161.Omoike A, Chorover J. Spectroscopic study of extracellular polymeric substances from Bacillus subtilis:aqueous chemistry and adsorption effects. Biomacromolecules, 2004,5:1219-1230
162.Otto K, Elwing H, Hermansson M. Effect of ionic strength on initial interactions of Escherichia coli with surfaces, studied on-line by a novel quartz crystal microbalance technique. Journal of Bacteriology,1999,181:5210-5218
163.Pachepsky Y, Yu O, Karns J, Shelton D, Guber A, Van Kessel J. Strain-dependent variations in attachment of E. coli to soil particles of different sizes. International Agrophysics,2008,22:61-66
164.Pachepsky YA, Sadeghi A, Bradford S, Shelton D, Guber A, Dao T. Transport and fate of manure-borne pathogens:modeling perspective. Agricultural Water Management,2006,86:81-92
165.Palmer J, Flint S, Brooks J. Bacterial cell attachment, the beginning of a biofilm. Journal of Industrial Microbiology & Biotechnology,2007,34:577-588
166.Parent ME, Velegol D. E. coli adhesion to silica in the presence of humic acid. Colloids and Surfaces B:Biointerfaces,2004,39:45-51
167.Parikh SJ, Chorover J. ATR-FTIR spectroscopy reveals bond formation during bacterial adhesion to iron oxide. Langmuir,2006,22:8492-8500
168.Park SJ, Kim SB. Adhesion of Escherichia coli to iron-coated sand in the presence of humic acid:a column experiment. Water Environment Research,2009,81:125-130
169.Paternostre G. Assessing the role of physicochemical and biochemical soil characteristics on Escherichia coli attachment. (Master dissertation). Washington, DC: Washington State University,2008
170.Patra P, Natarajan K. Microbially-induced flocculation and flotation for pyrite separation from oxide gangue minerals. Minerals Engineering,2003,16:965-973
171.Petridis H, Kidder G, Ogram A. E. coli O157:H7, a potential health concern. University of Florida Cooperative Extension Service, Institute of Food and Agriculture Sciences, EDIS,1998
172.Quirynen M, Bollen C. The influence of surface roughness and surface-free energy on supra-and subgingival plaque formation in man. Journal of Clinical Periodontology,1995,22:1-14
173.Redman JA, Estes MK, Grant SB. Resolving macroscale and microscale heterogeneity in virus filtration. Colloids and Surfaces A:Physicochemical and Engineering Aspects,2001,191:57-70
174.Redman JA, Walker SL, Elimelech M. Bacterial adhesion and transport in porous media:role of the secondary energy minimum. Environmental Science & Technology, 2004,38:1777-1785
175.Rijnaarts HH, Norde W, Lyklema J, Zehnder AJ. DLVO and steric contributions to bacterial deposition in media of different ionic strengths. Colloids and Surfaces B: Biointerfaces,1999,14:179-195
176.Rochex A, Lecouturier D, Pezron I, Lebeault JM. Adhesion of a Pseudomonas putida strain isolated from a paper machine to cellulose fibres. Applied Microbiology and Biotechnology,2004,65:727-733
177.Rong X, Chen W, Huang Q, Cai P, Liang W. Pseudomonas putida adhesion to goethite:studied by equilibrium adsorption, SEM, FTIR and ITC. Colloids and Surfaces B:Biointerfaces,2010,80:79-85
178.Rong X, Huang Q, Chen W. Microcalorimetric investigation on the metabolic activity of Bacillus thuringiensis as influenced by kaolinite, montmorillonite and goethite. Applied Clay Science,2007,38:97-103
179.Rong X, Huang Q, He X, Chen H, Cai P, Liang W. Interaction of Pseudomonas putida with kaolinite and montmorillonite:a combination study by equilibrium adsorption, ITC, SEM and FTIR. Colloids and Surfaces B:Biointerfaces,2008,64: 49-55
180.Saiers JE, Ryan JN. Introduction to special section on colloid transport in subsurface environments. Water Resources Research,2006,42, W12S01, DOI: 10.1029/2006WR005620
181.Santamaria J, Toranzos GA. Enteric pathogens and soil:a short review. International Microbiology,2003,6:5-9
182.Schafer A, Harms H, Zehnder AJ. Bacterial accumulation at the air-water interface. Environmental Science & Technology,1998,32:3704-3712
183.Schijven JF, Hoogenboezem W, Hassanizadeh SM, Peters JH. Modeling removal of bacteriophages MS2 and PRD1 by dune recharge at Castricum, Netherlands. Water Resources Research,1999,35:1101-1111
184.Schinner T, Letzner A, Liedtke S, Castro FD, Eydelnant IA, Tufenkji N. Transport of selected bacterial pathogens in agricultural soil and quartz sand. Water Research, 2010,44:1182-1192
185.Scholl MA, Harvey RW. Laboratory investigations on the role of sediment surface and groundwater chemistry in transport of bacteria through a contaminated sandy aquifer. Environmental Science & Technology,1992,26:1410-1417
186.Semenov AV, Van Overbeek L, van Bruggen AH. Percolation and survival of Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium in soil amended with contaminated dairy manure or slurry. Applied and Environmental Microbiology,2009,75:3206-3215
187.Sharma M, Chang Y, Yen T. Reversible and irreversible surface charge modification of bacteria for facilitating transport through porous media. Colloids and Surfaces, 1985,16:193-206
188.Shashikala A, Raichur A. Role of interfacial phenomena in determining adsorption of Bacillus polymyxa onto hematite and quartz. Colloids and Surfaces B:Biointerfaces, 2002,24:11-20
189.Shellenberger K, Logan BE. Effect of molecular scale roughness of glass beads on colloidal and bacterial deposition. Environmental Science & Technology,2002,36: 184-189
190.Shi X, Yu D, Sun W, Wang H, Zhao Q, Gong Z. Reference benchmarks relating to great groups of genetic soil classification of China with soil taxonomy. Chinese Science Bulletin,2004,49:1507-1511
191.Simon M, Garcia I. Physico-chemical properties of the soil-saturation extracts: estimation from electrical conductivity. Geoderma,1999,90:99-109
192.Simoni SF, Harms H, Bosma TN, Zehnder AJ. Population heterogeneity affects transport of bacteria through sand columns at low flow rates. Environmental Science & Technology,1998,32:2100-2105
193.Sinton L, Finlay R, Pang L, Scott D. Transport of bacteria and bacteriophages in irrigated effluent into and through an alluvial gravel aquifer. Water, Air,& Soil Pollution,1997,98:17-42
194.Smith M, Thomas G, White R, Ritonga D. Transport of Escherichia coli through intact and disturbed soil columns. Journal of Environmental Quality,1985,14:87-91
195.Sobel ME. Direct and indirect effects in linear structural equation models. Sociological Methods & Research,1987,16:155-176
196.Soupir ML, Mostaghimi S. Escherichia coli and Enterococci attachment to particles in runoff from highly and sparsely vegetated grassland. Water, Air, & Soil Pollution, 2010,216:167-178
197.Stenstrom T. Bacterial hydrophobicity, an overall parameter for the measurement of adhesion potential to soil particles. Applied and Environmental Microbiology,1989, 55:142-147
198.Stephenson M. Bacterial metabolism. London:Longmans, Green & Co,1949
199.Syngouna VI, Chrysikopoulos CV. Interaction between viruses and clays in static and dynamic batch systems. Environmental Science & Technology,2010,44:4539-4544
200.Tazehkand SS, Torkzaban S, Bradford SA, Walker SL. Cell preparation methods influence D21g surface chemistry and transport in saturated sand. Journal of Environmental Quality,2008,37:2108-2115
201.Tezuka Y. Cation-dependent flocculation in a flavobacterium species predominant in activated sludge. Applied Microbiology,1969,17:222-226
202.Tietjen T, Wetzel RG. Extracellular enzyme-clay mineral complexes:enzyme adsorption, alteration of enzyme activity, and protection from photodegradation. Aquatic Ecology,2003,37:331-339
203.Tisdall J, Oades J. Organic matter and water-stable aggregates in soils. Journal of Soil Science,2006,33:141-163
204.Tong M, Long G, Jiang X, Kim HN. Contribution of extracellular polymeric substances on representative gram negative and gram positive bacterial deposition in porous media. Environmental Science & Technology,2010,44:2393-2399
205.Tufenkji N, Elimelech M. Deviation from the classical colloid filtration theory in the presence of repulsive DLVO interactions. Langmuir,2004,20:10818-10828
206.Turner BF, Fein JB. Protofit:a program for determining surface protonation constants from titration data. Computers & Geosciences,2006,32:1344-1356
207.Ueshima M, Ginn BR, Haack EA, Szymanowski JE, Fein JB. Cd adsorption onto Pseudomonas putida in the presence and absence of extracellular polymeric substances. Geochimica et Cosmochimica Acta,2008,72:5885-5895
208.Unc A, Goss MJ. Movement of faecal bacteria through the vadose zone. Water, Air, & Soil Pollution,2003,149:327-337
209.Unc A, Goss MJ. Transport of bacteria from manure and protection of water resources. Applied Soil Ecology,2004,25:1-18
210.Vadillo-Rodriguez V, Busscher HJ, Mei HC, Vries Jd, Norde W. Role of lactobacillus cell surface hydrophobicity as probed by AFM in adhesion to surfaces at low and high ionic strength. Colloids and Surfaces B:Biointerfaces,2005,41:33-41
211.Van Kessel JS, Pachepsky YA, Shelton DR, Karns JS. Survival of Escherichia coli in cowpats in pasture and in laboratory conditions. Journal of Applied Microbiology, 2007,103:1122-1127
212.Van Loosdrecht M, Lyklema J, Norde W, Zehnder A. Influence of interfaces on microbial activity. Microbiological Reviews,1990,54:75-87
213.Van Loosdrecht M, Zehnder A. Energetics of bacterial adhesion. Cellular and Molecular Life Sciences,1990,46:817-822
214.Van Loosdrecht MC, Lyklema J, Norde W, Zehnder AJ. Bacterial adhesion:a physicochemical approach. Microbial Ecology,1989,17:1-15
215.Van Oss CJ. The forces involved in bioadhesion to flat surfaces and particles-their determination and relative roles. Biofouling,1991,4:25-35
216.Van Oss CJ. Hydrophobicity of biosurfaces-origin, quantitative determination and interaction energies. Colloids and Surfaces B:Biointerfaces,1995,5:91-110
217.Van Oss CJ, Chaudhury M, Good R. Monopolar surfaces. Advances in Colloid and Interface Science,1987,28:35-64
218.Van Oss CJ. Hydrophobicity and hydrophilicity of biosurfaces. Current Opinion in Colloid & Interface Science,1997,2:503-512
219.Vettori C, Stotzky G, Yoder M, Gallori E. Interaction between bacteriophage PBS1 and clay minerals and transduction of Bacillus subtilis by clay-phage complexes. Environmental Microbiology,1999,1:347-355
220.Vidovic SVS, Hushton C, Block BH, Korber DRKD. Effect of soil composition, temperature, indigenous microflora, and environmental conditions on the survival of Escherichia coli O157:H7. Canadian Journal of Microbiology,2007,53:822-829
221.Vigeant M, Ford RM. Interactions between motile Escherichia coli and glass in media with various ionic strengths, as observed with a three-dimensional-tracking microscope. Applied and Environmental Microbiology,1997,63:3474-3479
222.Vigeant MAS, Ford RM, Wagner M, Tamm LK. Reversible and irreversible adhesion of motile Escherichia coli cells analyzed by total internal reflection aqueous fluorescence microscopy. Applied and Environmental Microbiology,2002,68: 2794-2801
223.Walker SL, Hill JE, Redman JA, Elimelech M. Influence of growth phase on adhesion kinetics of Escherichia coli D21g. Applied and Environmental Microbiology,2005a,71:3093-3099
224.Walker SL, Redman JA, Elimelech M. Role of cell surface lipopolysaccharides in Escherichia coli K12 adhesion and transport. Langmuir,2004,20:7736-7746
225.Walker SL, Redman JA, Elimelech M. Influence of growth phase on bacterial deposition:interaction mechanisms in packed-bed column and radial stagnation point flow systems. Environmental Science & Technology,2005b,39:6405-6411
226.Wang L, Xu S, Li J. Effects of phosphate on the transport of Escherichia coli O157:H7 in saturated quartz sand. Environmental Science & Technology,2011,45: 9566-9573
227.Waters AG, Oades JM. Organic matter in water-stable aggregates. In:Wilson WS ed., Advances in Soil Organic Matter Research:the Impact on Agriculture and the Environment. The Royal Society of Chemistry, Cambridge,1991.163-174
228.Weiss TH, Mills AL, Hornberger GM, Herman JS. Effect of bacterial cell shape on transport of bacteria in porous media. Environmental Science & Technology,1995, 29:1737-1740
229.Wu H, Jiang D, Cai P, Rong X, Dai K, Liang W, Huang Q. Adsorption of Pseudomonas putida on soil particle size fractions:effects of solution chemistry and organic matter. Journal of Soils and Sediments,2012,12:143-149
230.Wu H, Jiang D, Cai P, Rong X, Huang Q. Effects of low-molecular-weight organic ligands and phosphate on adsorption of Pseudomonas putida by clay minerals and iron oxide. Colloids and Surfaces B:Biointerfaces,2011,82:147-151
231.Wu S, Nishihara M, Kawasaki Y, Yokoyama A, Matsuura K, Koga T, Ueno D, Inoue K, Someya T. Inactivation of Escherichia coli in soil by solarization. Soil Science and Plant Nutrition,2009,55:258-263
232.Yao KM, Habibian MT, O'Melia CR. Water and waste water filtration. Concepts and applications. Environmental Science & Technology,1971,5:1105-1112
233.Yavuz Corapcioglu M, Haridas A. Transport and fate of microorganisms in porous media:a theoretical investigation. Journal of Hydrology,1984,72:149-169
234.Yee N, Benning LG, Phoenix VR, Ferris FG. Characterization of metal-cyanobacteria sorption reactions:a combined macroscopic and infrared spectroscopic investigation. Environmental Science & Technology,2004,38:775-782
235.Yee N, Fein JB, Daughney CJ. Experimental study of the pH, ionic strength, and reversibility behavior of bacteria-mineral adsorption. Geochimica et Cosmochimica Acta,2000,64:609-617
236.You Y, Rankin SC, Aceto HW, Benson CE, Toth JD, Dou Z. Survival of Salmonella enterica serovar Newport in manure and manure-amended soils. Applied and Environmental Microbiology,2006,72:5777-5783
237.Zhao W, Liu X, Huang Q, Rong X, Liang W, Dai K, Cai P. Sorption of Streptococcus suis on various soil particles from an Alfisol and effects on pathogen metabolic activity. European Journal of Soil Science,2012a,63:558-564
238.Zhao W, Liu X, Huang Q, Walker SL, Cai P. Interactions of pathogens Escherichia coli and Streptococcus suis with clay minerals. Applied Clay Science,2012b,69: 37-42
239.Zhu P, Long G, Ni J, Tong M. Deposition kinetics of extracellular polymeric substances (EPS) on silica in monovalent and divalent salts. Environmental Science & Technology,2009,43:5699-5704
240.Zita A, Hermansson M. Effects of bacterial cell surface structures and hydrophobicity on attachment to activated sludge flocs. Applied and Environmental Microbiology, 1997,63:1168-1170
241.Zobell CE. The effect of solid surfaces upon bacterial activity. Journal of Bacteriology,1943,46:39-56