山羊多产性候选基因INHBA和INHBB基因的SNPs研究
摘要
本研究以高繁殖力山羊品种(济宁青山羊)和低繁殖力山羊品种(安哥拉山羊和内蒙古绒山羊)为对象,研究INHBA基因和INHBB基因的单链核苷酸多态性(single nucleotide polymorphisms,SNPs)及其与山羊繁殖力之间的关联性。根据GenBank中发表的牛INHBA基因的外显子1、外显子2和INHBB基因的外显子2序列分别设计4对和2对引物。采用PCR-SSCP技术分别检测济宁青山羊、内蒙古绒山羊和安哥拉山羊在这两个基因座中的SNPs。分析整个山羊群体在多态性基因座上的遗传结构及其与山羊多产性之间的关联。结果表明:
1.在6对引物中,有2对引物检测出SNPs;结果显示,INHBA基因(基因座A)外显子2和INHBB基因(基因座B)外显子2分别存在1个SNP位点。
2.在基因座A上,其突变位点位于外显子2第583 bp处,检测到一对等位基因A和B。在济宁青山羊和安哥拉山羊中检测到了AA、AB和BB 3种基因型,在内蒙古绒山羊中检测到AB和BB两种基因型。测序分析表明BB型与AA型相比在外显子2的第583 bp处有一个T→C突变,并引起氨基酸改变(脯氨酸→亮氨酸)。济宁青山羊AA、AB和BB基因型频率分别为0.4786、0.4143和0.1071。通过最小二乘均数比较发现,济宁青山羊AA型的产羔数比AB型多0.42只(P＜0.05),比BB型多1.14只(P＜0.01),AB型比BB型多0.72只(P＜0.05)
3.在基因座B上,其突变位点位于外显子2第583 bp处,检测到一对等位基因C和D。在济宁青山羊中检测到CC、CD和DD 3种基因型,在内蒙古绒山羊和安哥拉羊中只检测到CD和DD两种基因型。克隆测序发现DD型与CC型相比在外显子2的第840 bp处有A→G突变,并引起氨基酸改变(组氨酸→精氨酸)。济宁青山羊CC、CD和DD基因型频率分别为0.0685、0.500和0.4315。通过最小二乘均数比较发现,济宁青山羊CC型产羔数比CD型多0.73只(P＜0.05),比DD型多0.84只(P＜0.05),CD型比DD型多0.11只(P＞0.05)。
4.在2突变位点上,各山羊品种内和品种间存在遗传多态性。济宁青山羊在这2个位点上与其它山羊品种存在较大的遗传差异。可以认为这种遗传变异与山羊的多产性密切相关。
可见,INHBA基因和INHBB基因可能是控制山羊高繁殖力性状的主效基因,或与之存在一定程度的连锁。INHBA基因外显子2和INHBB基因外显子2的突变位点可以作为山羊多产性标记辅助选择的候选标记。
The purpose of this study was to detect single nucleotide polymorphisms(SNPs) within INHBA gene and INHBB gene,to analyze the genetic structure of three goat breeds,and to investigate the effects of the two genes on litter size of Jining Grey goats. Based on the bovine DNA sequences in GenBank,four and two primer pairs for INHBA gene and INHBB gene,respectively,were designed for PCR.Amplified regions included Exon 1,Exon 2 of INHBA gene.SNPs of INHBA and INHBB from three different goat breeds were detected by single strand configuration polymorphisms (SSCP).The genetic structure of the three populations at polymorphic loci,and the associations of the litter size of Jining Grey does with the two genes were also explored. The results are as follows:
1.By 6 pairs of primers,only 2 SNP sites were tested,which located respectively at exon 2 of INHBA(named locus A)and exon 2 of INHBB gene(named locus B).
2.At locus A,the mutation site was at 583 bp of the exon 2,which resulted in allele A and B.Sequencing revealed that allele B was caused by T→C mutation.This mutation resulted in proline→leucine(Pro→leu)substitution.Genotypes AA,AB and BB were detected in Jining Grey goats and Angora goats,genotypes AB and BB in Inner Mongolia Cashmere goats.AA,AB and BB genotype frequencies were 0.4786, 0.4143 and 0.1071 respectively in Jining Grey goats.The difference of the kids among the different genotyes In Jining Grey goat,showed that the kids of genotype AA increased 1.14(P＜0.01)than that of genotype BB and 0.42(P＜0.05)than that of genotype AB respectively;the kids with genotype AB increased 0.72(P＜0.05)than with genotype BB.
3.At locus B,the mutation site was at 840 bp of the exon 2,which resulted in allele C and D.Sequencing revealed that allele D was caused by A→G mutation.This mutation resulted in His→Arg substitution.Genotype CC,CD and DD were detected in Jining Grey,genotype CD and DD in both Inner Mongolia Cashmere goats and Angora goats.Genotype frequencies of CC,CD and DD were 0.0685,0.5000 and 0.4315 respectively in Jining Grey goats.In Jining Grey goat,the difference of the kids among the different genotyes showed that the kids of genotype CC increased 0.73(P＜0.05) than genotype CD and 0.84 than that of genotype DD respectively,the kids with genotype CD increased 0.11 than with genotype DD(P＞0.05).
4.The genetic polymorphism in 3 breeds at locus A and B was rich.Genetic difference at locus A and B between Jining Grey goat and other two goat breeds was significant,which was associated with the fecundity of goat.
Therefore,INHBA gene and INHBB gene may be regarded as major genes for the fecundity of goat,or linked with the major genes influencing the fecundity of goat.It may be feasible to implement the marker assisted selection using the two SNP sites.
1.在6对引物中,有2对引物检测出SNPs;结果显示,INHBA基因(基因座A)外显子2和INHBB基因(基因座B)外显子2分别存在1个SNP位点。
2.在基因座A上,其突变位点位于外显子2第583 bp处,检测到一对等位基因A和B。在济宁青山羊和安哥拉山羊中检测到了AA、AB和BB 3种基因型,在内蒙古绒山羊中检测到AB和BB两种基因型。测序分析表明BB型与AA型相比在外显子2的第583 bp处有一个T→C突变,并引起氨基酸改变(脯氨酸→亮氨酸)。济宁青山羊AA、AB和BB基因型频率分别为0.4786、0.4143和0.1071。通过最小二乘均数比较发现,济宁青山羊AA型的产羔数比AB型多0.42只(P＜0.05),比BB型多1.14只(P＜0.01),AB型比BB型多0.72只(P＜0.05)
3.在基因座B上,其突变位点位于外显子2第583 bp处,检测到一对等位基因C和D。在济宁青山羊中检测到CC、CD和DD 3种基因型,在内蒙古绒山羊和安哥拉羊中只检测到CD和DD两种基因型。克隆测序发现DD型与CC型相比在外显子2的第840 bp处有A→G突变,并引起氨基酸改变(组氨酸→精氨酸)。济宁青山羊CC、CD和DD基因型频率分别为0.0685、0.500和0.4315。通过最小二乘均数比较发现,济宁青山羊CC型产羔数比CD型多0.73只(P＜0.05),比DD型多0.84只(P＜0.05),CD型比DD型多0.11只(P＞0.05)。
4.在2突变位点上,各山羊品种内和品种间存在遗传多态性。济宁青山羊在这2个位点上与其它山羊品种存在较大的遗传差异。可以认为这种遗传变异与山羊的多产性密切相关。
可见,INHBA基因和INHBB基因可能是控制山羊高繁殖力性状的主效基因,或与之存在一定程度的连锁。INHBA基因外显子2和INHBB基因外显子2的突变位点可以作为山羊多产性标记辅助选择的候选标记。
The purpose of this study was to detect single nucleotide polymorphisms(SNPs) within INHBA gene and INHBB gene,to analyze the genetic structure of three goat breeds,and to investigate the effects of the two genes on litter size of Jining Grey goats. Based on the bovine DNA sequences in GenBank,four and two primer pairs for INHBA gene and INHBB gene,respectively,were designed for PCR.Amplified regions included Exon 1,Exon 2 of INHBA gene.SNPs of INHBA and INHBB from three different goat breeds were detected by single strand configuration polymorphisms (SSCP).The genetic structure of the three populations at polymorphic loci,and the associations of the litter size of Jining Grey does with the two genes were also explored. The results are as follows:
1.By 6 pairs of primers,only 2 SNP sites were tested,which located respectively at exon 2 of INHBA(named locus A)and exon 2 of INHBB gene(named locus B).
2.At locus A,the mutation site was at 583 bp of the exon 2,which resulted in allele A and B.Sequencing revealed that allele B was caused by T→C mutation.This mutation resulted in proline→leucine(Pro→leu)substitution.Genotypes AA,AB and BB were detected in Jining Grey goats and Angora goats,genotypes AB and BB in Inner Mongolia Cashmere goats.AA,AB and BB genotype frequencies were 0.4786, 0.4143 and 0.1071 respectively in Jining Grey goats.The difference of the kids among the different genotyes In Jining Grey goat,showed that the kids of genotype AA increased 1.14(P＜0.01)than that of genotype BB and 0.42(P＜0.05)than that of genotype AB respectively;the kids with genotype AB increased 0.72(P＜0.05)than with genotype BB.
3.At locus B,the mutation site was at 840 bp of the exon 2,which resulted in allele C and D.Sequencing revealed that allele D was caused by A→G mutation.This mutation resulted in His→Arg substitution.Genotype CC,CD and DD were detected in Jining Grey,genotype CD and DD in both Inner Mongolia Cashmere goats and Angora goats.Genotype frequencies of CC,CD and DD were 0.0685,0.5000 and 0.4315 respectively in Jining Grey goats.In Jining Grey goat,the difference of the kids among the different genotyes showed that the kids of genotype CC increased 0.73(P＜0.05) than genotype CD and 0.84 than that of genotype DD respectively,the kids with genotype CD increased 0.11 than with genotype DD(P＞0.05).
4.The genetic polymorphism in 3 breeds at locus A and B was rich.Genetic difference at locus A and B between Jining Grey goat and other two goat breeds was significant,which was associated with the fecundity of goat.
Therefore,INHBA gene and INHBB gene may be regarded as major genes for the fecundity of goat,or linked with the major genes influencing the fecundity of goat.It may be feasible to implement the marker assisted selection using the two SNP sites.
引文
1.Frisch J E.Identification of a major gene for resistance to cattle ticks[C].In:Bumside E B ed.Proceeding of 5th world congress on genetics applied to livestock Production.Guelph:university of guelph,1994,20:293-295.
2.Piper L R,Bindon B M,Nethery R D.The Booroola Merino,Melbourne:CSIRO Division of Animal production[C],1982,9-20.
3.Bodin L,Elsen J M,Poivey J P,San Cristobal-Gaudy M,Belloc J P,Eychenne F.Hyper prolificacy in the French La caune sheep breed-A possible major gene[C].In:Laurie Piper ed.Proceedings of 6 WCGALP.NSW Australia:University of New England,1998,27:11-14.
4.施启顺.畜禽主基因研究进展[J].湖南农业大学学报,1999,25(1):81-86.
5.陈红菊,岳永生.动物主基因的研究进展[J].山东备牧兽医,2001(6):35-37.
6.曹果清,周忠孝,袁建霞,朱向芳.猪主要经济性状候选基因的研究进展,遗传,2002,24(2):214-218.
7.柴丽娟,赵先萍,刘文忠.绵羊主基因的研究进展[J].中国畜牧杂志,2003,39(3):51-52.
8.徐华,孙少华.畜禽主基因的研究现状与检测方法[J].当代畜牧,2004,2:46-49.
9.耿荣庆,王兰萍.我国绵羊多胎性能的研究与利用概况[J].畜禽业,2001(5):6-7.
10.耿荣庆.我国绵羊多胎性能主基因的开发利用[J].河南畜牧兽医,2001,22(1):13-13.
11.薛慧良,周忠孝.绵羊繁殖性状候选基因的研究进展[J].动物科学与动物医学,2003,93(3):32-35.
12.张秀华,吴登俊.绵羊繁殖性状的研究进展[J].草食家畜,2003(1):19-21.
13.季从亮,储明星,陈国宏,周国利,朱颜.4个绵羊品种褪黑激素受体1a基因第二外显子PCR-RFLP分析[J].华中农业大学学报,2003,22(2):105-109.
14.李碧侠,储明星,王金玉.绵羊GDF9基因PCR-SSCP分析[J].遗传学报,2003,30(4):307-310.
15.Chu M X,Ji C L,Chen G H.Association between PCR-RFLP of melatonin receptor 1a gene and high prolificacy in Small Tail Han sheep[J].Asian-Aust J Anim Sci,2003,16(12):1701-1704.
16.Guo W,Chu M X,Deng X M,Feng J D,Li Ning,Wu Changxin.Association of a single codon deletion in bone morphogenetic protein 15 gene with prolificacy in Small Tail Han sheep[J].Asian-A ust.J Anim Sci,2004,17(11):1491-1495.
17.朱靖,杜立新.小尾寒羊多胎性候选基因的研究进展[J].动物科学与动物医学2004,21(9):16-18.
18.储明星.利用分子育种技术选育肉用多胎绵羊新品系[J].中国农业科技导报,2004,6(6):15-19.
19.Chu M X,Li B X,Wang J Y,Ye S C,Fang L.Association between PCR-SSCP of growth differentiation factor 9 gene and high prolificacy in Small Tail Han sheep[J].Anita Biotechnol,2004,15(2):111-120.
20.Yan Y D,Chu M X,Zeng Y Q,Fang L,Ye S C,Wang L M,Guo Q K,Han D Q,Zhang Z X,Wang X J,Zhang X Z.Bone morphogenetie protein receptor IB as a candidate gene for prolificacy in Small Tail Han and Hu ewes[J].Chinese Journal of Agricultural Biotechnology,2005,2(2):125-130.
21.储明星,桑林华,王金玉,方丽,叶素成.小尾寒羊高繁殖力候选基冈BMPl5和GDF9的研究[J].遗传学报,2005,32(1):38-45.
22.毕晓丹,储明星,金海国,方丽,叶素成.小尾寒羊高繁殖力候选基因ESR的研究[J].遗传学报,2005,32(10):1060-1065.
23.闫亚东,储明星,曾勇庆,方丽,叶素成,王利民,国庆坤,韩代勤,张兆新,王西筠,张新珍.小尾寒羊和湖羊高繁殖力候选基因BMPR-IB的研究[J].农业生物技术学报,2005,13(1):66-71.
24.储明星,何远清,王金玉,Olowofeso O,叶素成,方丽.绵羊肌细胞生成素基因外显子1的PCR-SSCP分析[J].农业生物技术学报,2005,13(1):77-80.
25.储明星,成荣,陈国宏,方丽,叶素成.小尾寒羊和湖羊高繁殖力候选基因BMP15的研究[J].安徽农业大学学报,2005,32(3):278-282.
26.艾君涛,管峰,杨利国.绵羊繁殖性状主基因研究进展[J].中国农学通报,2005,21(6):39-41
27.Dube J L,Wang P,Elvin J.The bone morphogenetic protein15 gene is linked and expressed in oocytes[J].Molecular Endocrinology,1998,12(2):1809-1817.
28.Otsuka F.Bone morphogenetic protein 15 inhibits follicle stimulating hormone(FSH)action by suppressing FSH receptor expression[J].Biol Chem,2001,276(14):1187-1192.
29.Galloway S M,McNatty K P,Cambridge L M.Mutations in an oocyte derived growth diferentiation factor gene(BMPI5)cause increased ovulation rate and infertility in a dosage-sensitive manner[J].Nature Genetics,2000,25(3):279-283.
30.Yan C N,Wang P,DeMayo J,Demayo F J,Elvin J A,Carino C,Prasad S V,Skinner S S,Dunbar B S,Dube L J,Celeste A J,Matzuk M M.Synergistic roles of bone morphogenetic proein 15 and growth differentiation factor 9 in ovarian function[J].Mol Endocrinol,2001,15(6):854-866.
31.Juengel J L,Hudson N L,Heath D A,Smith P,Reader K L,Lawrence S B,O'Connell A R,Laitinen M P,Cranfield M,Groome N P,Ritvos O,McNatty K P.Growth differentiation factor 9 and bone morphogenetic protein 15 are essential for ovarian follicular development in sheep [J].Biol Reprod,2002,67(6):1777-1789.
32.Hanrahan J P,Gregan S M,Mulsant P,Muilen M,Davis G H,Powell R,Galloway S M.Mutations in the genes for oocyte-derived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in Cambridge and Belclare sheep(Ovis aries)[J].Biol Reprod,2004,70(4):900-909.
33.何远清,储明星,王金玉,方丽,叶素成.6个山羊品种高繁殖力候选基因BMP15多态性研究.安徽农业大学学报[J].2006,33(1):61-64.
34.吴泽辉,储明星,李学伟.生长分化因子9基因及其在生殖中的作用[J].遗传,2005,27(3):481-486.
35.Kaivo-Oja N,Bondestam J,Kamarainen M,Koskimies J,Vitt U,Cranfield M,Vuojolainen K,Kallio J P,Olkkonen V M,Hayashi M,Moustakas A,Groome N P,ten Dijke P,Hsueh A J,Ritvos O.Growth differentiation factor-9 induces Smad2 activation and inhibin B production in human granulose-luteal cells[J].J Clin Endocrinol Metab,2003,88(2):755-762.
36.Dong J W,Albertini D F,Nishimori K,Kumar T R,Lu N,Matzuk M M.Growth differentiation factor 9 is required during early ovarian folliculogenesis[J].Nature,1996,383(6600):531-535.
37.Bodensteiner K J,Clay C M,Moeller C L,Sawyer H R.Molecular cloning of the ovine growth/differentiation factor-9 gene and expression of growth/differentiation factor-9 in ovine and bovine ovaries[J].Biol Reprod,1999,60(2):381-386.
38.Juengel J L,Hudson N L,Heath D A,Smith P,Reader K L,Lawrence S B,O'Connell A R,Laitinen M P,Cranfield M,Groome N P,Ritvos O,McNatty K P.Growth differentiation factor 9 and bone morphogenetic protein 15 are essential for ovarian follicular development in sheep [J].Biol Reprod,2002,67(6):1777-1789.
39.Juengel J L,Hudson N L,Whiting L,McNatty K P.Effects of immunization against bone morphogenetic protein 15 and growth differentiation factor 9 on ovulation rate,fertilization,and pregnancy in ewes[J].Biol Reprod,2004,70(3):557-561.
40.Mc Pherron A C,Lee S J.GDF-3 and GDF-9:two new members of the transforming growth factor-beta superfamily containing a novel pattern of cysteines[J].J Biol Chem,1993,268(5):3444-3449.
41.Incerti B,Dong J,Borsani G,Matzuk M M.Structure of the mouse growth-differentiation factor 9 gene[J].Biochem Biophys Acta,1994,1222(1):125-128.
42.杨晶.小尾寒羊GDF9基因和BMP15基因多态性及其与高繁殖力关系的研究.中国农业科学院硕士论文[D],2006.6.
43.何远清,储明星,王金玉,方丽,叶素成.山羊高繁殖力候选基冈GDF9的RFLP分忻[J].农业生物技术学报,2006,14(1):135-136.
44.吴泽辉,储明星,李学伟,方丽,叶素成,刘忠慧,陈国宏.山羊生长分化因子9基因外显子2的PCR-SSCP分析[J].中国农业科学,2006,39(4):802-808.
45.WolfJ B,David V A,Deutch A H.Identification of a distal regulatory element in the 5' flanking region of the bovine prolactin gene[J].Nucleic Acids Res,1990,18(16):4905-44912.
46.Kansaku N.Genetic variation of chicken prolactin promoter[M].Published Only in DataBase (2000).
47.崔建勋,梁勇,邓学梅,张豪,杨关福,张细权.鸡催乳素基因5'侧翼调控区近端启动子序列多态性及其与产蛋性能的关系[C].第十一次全国家禽学术讨论会论文集,长春:吉林科学技术出版社.2003,33-36.
48.Zhang H M,Denise S K,Ax R L.Diallielic single-stranded conformational polymorphism detected in the bovine prolactin gene[J].J Anim Sci,1994,72(1):256-257.
49.Vincent A L,Rothschild M E A restriction fragment length polymorphism in the ovine prolactin gene[J].J Anim Sci,1997,75(6):1686.
50.储明星.猪产仔数性状候选基因的研究进展[J].畜牧与兽医,2001,33(1):36-37.
51.阮征,凌家瑞.以催乳素受体基因(PRLR)作为母猪产仔数候选基因的分析[J].上海畜牧兽医通讯2002(1):20-21.
52.李国治,连林生,鲁绍雄,严达伟.以催乳素受体基因作为撒坝猪部分生产性能候选基因 的研究[J].养猪,2005(4):36-38.
53.张冬杰,沙伟,杨国伟,刘娣.催乳素受体基因(PRLR)与产仔数关系的研究[J].安徽农业科学,2005,33(12):2340-2341.
54.曹果清,李步高,刘建华,杨文平.猪催乳素受体(PRLR)基冈Alu Ⅰ多态性与产仔数关系的研究[J].山西农业大学学报,2004,24(3):253-255.
55.银晶.山羊催乳素受体基因内含子Ⅰ和Ⅱ单链构象多态性与产羔性能关系的研究.山西农业大学硕士学位论文[D].2004.12.
56.Reppert S M,Weaver D R,Ebisawa T.Cloning and characterization of a mammalian receptor that mediates reproductive and circadian responses[J].Neuron,1994,13(5):1177-1185.
57.Skinner T M,Lopez-Corrales N L,Anderson S I.Genetic and physical mapping of the porcine melatonin receptorlB gene(MTNRIB)to chromosome 9[J].Cytogenet Cell Genet,2000,90(2):53-55.
58.Slaugenhaupt S A,Roca A L,Liebert C B,Aitherr M R,Gusella J F,Reppert S M.Mapping of the gene for the Mella-melatonin receptor to human chromosome 4(MTNRIA)and mouse chromosome 8(Mtnrla)[J].Genomics,1995,27(2):355-357.
59.Messer L A,Wang L Z,Tuggle C K.Mapping of the melatonin receptor la(MTNR1A)gene in pigs,sheep,and cattle[J].Mammalian Genome,1997,8(5):368-370.
60.Pelletier J,Bodin L,Hanocq E,Malpaux B,Teyssier J,Thimonier J,Chemineau P.Association between expression of reproductive seasonality and alleles of the gene for Mella receptor in the ewe[J].Biol Reprod,2000,62(4):1096-1101.
61.季从亮,储明星,陈国宏.4个绵羊品种褪黑激素受体1a基因底二外显子PCR-RFLP分析[J].华中农业大学学报,2003,22(2):105-109.
62.Migaud M,Gavet S,Pelletier J.Partial cloning and polymorphism of the melatoninla(Mella)receptor gene in two breeds of goat with different reproductive seasonality[J].Repro-dution,2002,124(1):59-64.
63.Buger H G.lnhibin:definition and nomenclature including related substances[J].J Endocrinol,1988,117(2):159-160.
64.Leversha LJ,Robertson D M,de Vos F L,Morgan F J,Hearn M T,Wettenhall RE,Findlay JK,Burger HG,de Kretser D M.Isolation of inhibin from ovine follicular fluid.J Endocrinol.1987May;113(2):213-221.
65.路长林.神经肽基础与临床.重庆[M]:第三军医大学出版社,2000.
66.Vale W,Hsueh A,Rivier C,Yu J.The inhibin/activin family of hormones and growth factors,in Handbook of experimental pharmacology:peptide growth factors and their receptor Ⅱ Sporn[J].edvol.1990,95:211-248.
67.Ying S Y.lnhibins and activins,chemical properties and biological activity.Proc Soc Exp Biol Med.1987,186(3):253-264.
68.Ying S Y.Inhibins,activins,and follistatins:gonadal proteins modulating the secretion of follicle-stimulating hormone[J].Endocr Rev,1988,9(2):267-293.
69.Findlay J K.An update on the roles of inhibin,activins and follistain as local regular of folliculogenesis[J].Bio Reprod,1993,48(1):15-23.
70. Mather J P, Woodruff T K. Paracrine regular on reproductive function by inhibin and activin[J].Pro Sci Exp Biol Med, 1992,201(1):1-3.
71. Adams M H, Baker C B, McDowell K J. Molecular cloning and sequencing of equine inhibin alpha cDNA[J].Animal Biotechnology, 1996,7(1):1-9.
72 Hiendleder S, Lewalski H, Jaeger C, Pracht P, Erhardt G. Nucleotide sequence of ovine α-inhibin (INHA) genes and evaluation of RFLP marker effects on reproductive performance[J].Animal Genetics, 1996, 27(Suppl.2):91-92.
73. Hiendleder S, Lewalski H, Jaeger C, Plante Y. Genomic cloning and comparative sequence analysis of different alleles of the ovine β_A- inhibin/activin (INHBA) gene as a potential QTL for litter size[J]. Animal Genetics, 1996,27(Suppl.2):119.
74. Forage R G, Ring J M, Brown R W. Cloning and sequence analysis of cDNA species coding for the two subunits of inhibin from bovine follicular fluid[J]. Proc Natl Acad Sci U S A, 1986,83(10): 3091-3095.
75. Rodgers R J. Cloning of the inhibin/activin beta B subunit gene from the Booroola merino sheep[J]. J Mol Endocrinol, 1991,6(1):87-93.
76. Mason A J, Hayflick J S, Ling N. Complementary DNA sequences of ovarian follicular fluid inhibin show precursor structure and homology with transforming growth factor- beta[J].Nature, 1985, 318(6047):659-663.
77. Mason A J, Niafl H D, Seeburg P H. Structure of two human ovarian inhibins[J]. Biochem Biophys Res Commun,1986,135:957-964
78. Woodruff T K, Meunier H, Jones P B. Rat inhibin: molecular cloning of alpha- and beta-subunit complementary deoxyribonucleic acids and expression in the ovary[J]. Mol Endocrinol,1987, 1(8):561-568.
79. Barton D E, Yang-Feng T L, Mason A J, Seeburg P H, Francke U. Mapping of genes for inhibin subunits α, β_A and β_B on human and mouse chromosomes and studies of jsd mice[J]. Genomics.1989,5(1):91-99.
80. Lafuse W P, Zwilling B S. Localization of the inhibin β_B gene on mouse chromosome 1[J].Mammalian Genome, 1993,4(7):399—400.
81. Neibergs H L, Gallagher D S, Georges M, Sargeant L S, Dietz A B, Womack J E. Physical mapping of inhibin β_A in domestic cattle[J]. Mammalian Genome, 1993,4(6):328—332.
82 Brunner R M, Goldammer T, Hiendleder S, Jager C, Schwerin M. Mapping of the gene encoding for inhibin-βA (INHBA) to chromosome 4q26 in sheep[J]. Mammalian Genome,1995, 6(4):308-309.
83. Brunner R M, Goldammer T, Hiendleder S, Jager C, Schwerin M. Comparative mapping of the gene coding for inhibin-α (INHA) to chromosome 2 in sheep and cattle[J]. Mammalian Genome, 1995, 6(9):685-686.
84. Goldammer T, Brunner R M, Hiendleder S, Schwerin M. Comparative mapping of sheep inhibin subunit β_b to chromosome 2 in sheep and cattle by fluorescence in situ -hybridization[J]. Animal Genetics, 1995, 26(3): 199-200.
85. Goldammer T, Brunner R M, Hiendleder S, Schwerin M. Comparative mapping of sheep inhibin subunits α (INHA) and β_B (INHBB) to chromosome 2 in goat by FISH[J]. Mammalian Genome, 1995, 6(9):685-686.
86. Hiendleder S, Leyhe B, Jaeger C,Erhardt G and Wassmuth R. Molecular characterization of ovine α-, β_A- and β_B-inhibin/activin alleles[J]. Journal of Animal Breeding and Genetics, 1996,113:363-372.
87. Roberts V J, Sawchenko P E, Vale W. Expression of inhibin/activin subunit messenger ribonucleic acids during rat embryogenesis[J]. Endocrinology, 1991,128(6):3122—3129.
88. Roberts V J, Barth S, el-Roeiy A, Yen S S. Expression of inhibin/activin system messenger ribonucleic acids and proteins in ovarian follicles from women with polycystic ovarian syndrome[J]. J Clin Endocrinol Metab, 1994,79(5):1434-1439.
89. Drummond A E, Dyson M, Mercer J E, Findlay J K. Differential responses of post-natal rat ovarian cells to FSH and activin[J]. Mol Cell Endocrinol, 1996,122(1):21 -32.
90. Drummond A E, Dyson M, Thean E, Groome N P, Robertson D M, Findlay J K. Temporal and hormonal regulation of inhibin protein and subunit mRNA expression by post-natal and immature rat ovaries[J]. J Endocrinol, 2000,166(2):339-354.
91. Engelhardt H, Harkness L M, Thomas G B, Brooks A N, McNeilly A S, Baird D T. Expression of inhibin alpha- and beta A-subunit mRNA and protein in the fetal sheep ovary throughout gestation[J]. Mol Cell Endocrinol, 1995,107(2): 141-147.
92. Nambo Y, Nagata S, Oikawa M, Yoshihara T, Tsunoda N, Kohsaka T, Taniyama H, Watanabe G,Taya K. High concentrations of immunoreactive inhibin in the plasma of mares and fetal gonads during the second half of pregnancy[J]. Reprod Fertil Dev, 1996, 8(8): 1137— 1145.
93. Torney A H, Robertson D M, Hodgson Y M, de Kretser D M. In vitro bioactive and immunoactive inhibin concentrations in bovine fetal ovaries and testes throughout gestation[J].Endocrinology, 1990,127(6):2938-2946.
94. Chen C C, Johnson P A. Expression of inhibin alpha and inhibin/activin beta A subunits in the granulosa layer of the large preovulatory follicles of the hen[J]. Biol Reprod, 1996, 55(2):450-454.
95. Fleming J S, Galloway S M, Crawford R J, Tisdall D J, Greenwood P J. Tissue-specific variation in the length of the 5' untranslated region of the beta A-inhibin mRNA in sheep[J].Mol Reprod Dev, 1995, 40(1):1-8.
96. Bernard D J, ChapmanS C, Woodruff T K. Mechanisms of inhibin signal transduction[J].Recent Prog Horm Res, 2001, 56:417-450.
97. Eramaa M, Ritvos O. Transforming growth factor-beta 1 and -beta 2 induce inhibin and activin beta B-subunit messenger ribonucleic acid levels in cultured human granulosa-luteal cells[J].Fertil Steril, 1996,65(5): 954-960.
98. Kubo T, Shimasaki S, Kim H, Li D, Erickson G F. Activin-induced inhibin alpha-subunit production by rat granulosa cells requires endogenous insulin-like growth factor-I [J]. Biol Reprod, 1998,58(3):712-718.
99. Tamura K, Hatsuta M, Watanabe G, Taya K, Kogo H. Inhibitory regulation of inhibin gene expression by thyroid hormone during ovarian development in immature rats[J]. Biochem Biophys Res Commun, 1998, 242(1):102-108.
100. Debieve F, Thomas K. Control of the human inhibin alpha chain promoter in cytotrophoblast cells differentiating into syncytium[J]. Mol Hum Reprod,2002, 8(3):262-270.
101. Smyth C D, Gosden R G,McNeillyA S. Effect of inhibin immunoneutralization on steroidogenesis in ratovarian follicles in vitro[J]. Endocrinol, 1994, 140(3): 437—443.
102 Ying S Y, Czvik J, Becker A. Secretion of follicle-stim-ulating horm one and production of inhibin are reciprocally related[J]. Proc Natl Acad Sci, U S A, 1 987,84(13):4631 —4635.
103. Hillier S G, Yong E L, Illingworth P J. Effect of recombinant inhibin on androgen syn thesis in cultured human thecacells[J]. Mol Cell Endocrinol,1991,75(2):R(?)—6.
104. Tannetta D S, Feist S A, Bleach E C, Groome N P, Evans L W, Knight P G. Effects of active immunization of sheep against an amino terminal peptide of the inhibin alpha C subunit on intrafollicular levels of activin A,inhibin A and follistatin[J].J Endocrinol,1998,157(1):157-168.
105.张德坤.应用抑制素基因免疫技术诱导单胎绵羊孪生及其影响因素与作用机制研究[D].南京农业大学博士论文,2004
106.姜勋平,杨利国,刘桂琼.抑制素基因免疫对小鼠生殖的影响[J].中国兽医学报,2002,22(4):368-369.
107.周虚,田谷一善.抑制素被动免疫对黄体中期山羊生殖激素分泌的影响[J].中国兽医学报,2002,22(4):370-372.
108.Price C A,Morals B A,Oshea T,Webb R.Active immunization of cattle against partially purified follicular fluid from sheep[J].J Reprod Fert,1987,81(1):161-168.
109.Takedomi T,Kishi H,Medan M S,Aoyagi Y,Konishi M,Itoh T,Yazawa S,Watanabe G,Taya K.Active immunization against inhibin improves superovulatory response to exogenous FSH in cattle[J].J Reprod Dev,2005,51(3):341-346.
110.Medan M S,Akagi S,Kaneko H,Watanabe G,Tsonis C G,Taya K.Effects of re-immunization of heifers against inhibin on hormonal profiles and ovulation rate[J].Reproduction,2004,128(4):475-482
111.Schanbacher B D,Schemm S R,Rhind S M.Gonadotrophin concentrations and ovulation rates in Suffolk ewes actively of passively immunized against inhibin alpha[J].Reprod Fert,1991,93(1):133-139.
112.Medan M S,Takedomi T,Aoyagi Y,Konishi M,Yazawa S,Watanabe G,Taya K.The effect of active immunization agaist inhibin on Gonadotropin secretions and follicular dynamics during the estrous cycle in cows[J].J Reprod Dev,2006,52(1):107-113.
113.Brown R W,Hungerford J W,Greenword P E,Bloor R J,Evans D F,Tsonis C G,Forage R G.Immunization against recombinant bovine inhibin a subunit causes increased ovulation rates in gilts[J].Reprod Fert,1990,90(1):199-205.
114.Wheaton J E,Meyer R L,Jones R H,Kramer A J.Effects of passive immunization using antibody against an aipha-inhibin petptide on follicle-stimulating hormone concentrations and litter size in sows[J].Therogenology,1998,49(4):813-822.
115.Alabart JL,Folch J,Cocero MJ,Cognie Y.Effect of immunization on reproducvive performance,embryo quality and progesterone in Rasa Aragonesa ewes actively immunized against androstenedione or passively immunized against testosterone.Theriogenology.1991,35(4):799-813.
116.Medan M S,Watanabe G,Nagura Y,Kanazawa H,Fujita M,Taya K.Passive immunoneutralization of endogenous inhibin increase ovulation rate in miniature Shiba goats[J].J Reprod Dev,2004,50(6):705-710.
117.华进联.抑制素免疫对羊生殖的影响[J].畜牧兽医杂志,2000,19(3):15-17.
118.D'Alessandro A,Martemucci G,Iaffaldano N.Active immunization with a synthetic fragment of pig inhibin alpha-subunit increases ovulation rate and embryo production in superovulated ewes but season affects its efficiency[J].J Reprod Fertil,1999,115(1):185-191.
119.王杏龙,Wolfgang Holtz.抑制素免疫山羊卵巢对外源性促性腺激素的反应[J].中国备牧 杂志,2003,39(1):3-4.
120.刘娜.抑制素免疫提高家畜的排卵率[J].畜牧兽医杂志,1996,15:92-93.
121.Lovell T M,Knight P G,Groome N P,Gladwell R T.Changes in plasma inhibin A level during sexual maturation in the female chicken and the effects of active immunization against inhibin alpha-subunit on reproductive hormone profiles and ovarian function[J].Biol Reprod,2001,64(1):188-196.
122.Ahn J,You S,Kim H,Chaiseha Y,El Halawani M.Effects of active immunization with inhibin alpha subunit on reproductive characteristics of turkey hens[J].Biol Rerod,2001,65(5):1594-1600.
123.王进荣,杨利国,王元兴.人抑制家主动免疫对母鸡产蛋和内分泌的影响[J].中国畜牧杂志,2001,37(2):9-11.
124.Larsen N J,Nielsen V H.Apa I and Cfo I polymorphisms in the porcine growth hormone gene[J].Animal Genetics,1993,24(1):71
125.Neyroud N,Denjoy I,Donger C,Gary F,Villain E,Leenhardt A,Benali K,Schwartz K,Coumel P,Guicheney P.Heterozygous mutation in the pore of potassium channel gene KvLQT1 causes an apparently normal phenotype in long QT syndrome[J].Eur J Hum Genet,1998,6(2):129-133.
126.方美英,姜志华,刘红林,黄月英,李齐贤,朱婉茹,李学斌,陈杰.应用 PCR-RFLP、PCR-SSCP检测猪的氟烷基因[J].养猪,]997,8(1):29-29.
127.刘佳建,李奎,彭中镇.甘油浓度对PCR-SSCP分辨效果的影响[J].生物技术通讯,1998,6(2):160-161
128.姜运良,李宁.小片段DNA未知SNPs的PCR-SSCP检测方法分析[J].中国生物化学与分子生物学报2002,18(6):674-678..
129.Yao J B,Aggrey S E,Zadwomy D,Hayes J F,Kuhnlein U.Sequence variations in the bovine growth hormone gene characterized by sing-strand conformation polymorphism(sscp)analysis and their association with milk production traits in Holsteins[J].Genetics,1996,144(4):1809-1816.
130.Marklund S,Chaudhary R,Marklund L,Sandberg K,Andersson L.Extensive mtDNA diversity in horses revealed by PCR-SSCP analysis[J].Anim Genet,1995,26(3):193-196.
131.Maddox R Z.Amale linkage map of the cattle(Bos Taurus)genome[J].Journal of Heredity,1996,87:261-271.
132.赵兴波.储明星.绵羊线粒体DNA控制区5'端序列PCR-SSCP与序列分析[J].遗传学报,2001,28(3):225-228.
133.李碧侠,储明星,王金玉.绵羊GDF9基因PCR-SSCP分析[J].遗传学报,2003,30(4):307-310.
134.闫亚东,储明星,曾勇庆,方丽,叶素成,王利民,国庆坤,韩代勤,张兆新,王西筠,张新珍.小尾寒羊和湖羊高繁殖力候选基因BMPR-IB的研究[J].农业生物技术学报,2005,13(1):66-71.
135.储明星,何远清,王金玉,Olowofeso Olajide,叶素成,方丽.绵羊肌细胞生成素基因外显子1的PCR-SSCP分析[J].农业生物技术学报,2005,13(1):77-80.
136.中国农业信息网数据资料.http://www.agri.gov.cn/sjzl/
137.1988,88-90,98-100.《中国羊品种志》编写组.中国羊品种志.上海:上海科学技术出版社,1988,88-90,98-100.
138.Roberts A J,Reeves J J.Kidding rates of Angora goats passively immunized against estrogens [J].J Anim Sci,1988,66(10):2443-2447.
139.Thompson D A,Cronin C N,Martin F.Genomic cloning and sequence analyses of the bovine alpha-,beta A- and beta B-inhibin/activin genes.Identification of transcription factor AP-2-binding sites in the 5'-flanking regions by DNase I footprinting[J].Eur J Biochem,1994,226(3):751-764.
140.Don R H,Cox P T,Wainwright B J,Baker K,Mattick J S.Touchdown'PCR to circumvent spurious priming during gene amplification[J].Nucleic Acids Res,1991,19(14):4008.
141.Bostin D,White R L,Skolnick M,Davis R W.Construction of a genetic linkage map in man using restriction fragment length polymouphism[J].American Journal of Human Genetic,1980,32:314-331.
142.Weir B S,Cockerham C C.Estimating F-statistics for the analysis of population structure[J].Evolution,2002,36:721-750.
143.Hayashi K.PCR-SSCP:A mothed for detection of mutation.GATA,1992,9(3):73-79.
144.卢圣栋.现代分子生物学试验技术[M].北京:高等教育出版社,1993,95-103.
145.张学,孙开来.PCR-SSCP分析技术及其应用[J].国外医学遗传学分册,1992,5(5):225-231.
146.根井正利 著.王家玉,译.分子群体遗传学与进化论[M].北京:北京农业出版社.1985:15-154.
147.常洪.家畜遗传资源纲要[M].北京:中国农业出版社.1995:28-135.
148.刘文忠,张沅.安哥拉山羊育种和生产中的非遗传因素[J].中国草食动物,2004,24(1):46-48.
149.刘文忠,张沅.安哥拉山羊的遗传改良[J].中国畜牧兽医,2004,31(3):27-30
150.Nei Masatoshi.Molecular population genetic and evolution[M].Beijing.Agricultural Publishing House.1985,15-154.
151.欧刚叙向,邓灶福,黄生强.微卫星标记BM143在4个山羊品种中的遗传多样性分析[J].经济动物学报,2005,9(2):103-106
152.侯冠或,马月辉,王东劲.6个绵羊群体遗传分化研究[J].热带农业科学,2005,25(5):33-35.
153.杨章平,常洪,孙伟.结构基因座和微卫星标记应用于绵(山)羊群体遗传分化的比较研究[J].畜牧兽医学报,2003,34(5):427-433
154.张增利,巩元芳,刘铮铸.我国主要地方绵羊品种遗传亲缘关系[J].中国兽医学报,2004,24(5):508-511.
155.汪志国,杨章平,马月辉,王庆华,毛永江,常洪,周群兰,许明.长江中下游以及东南沿海的7个山羊群体的遗传多样性分析[J].畜牧兽医学报,2006,37(1),1-6.
156.Hiendleder S,Leyhe B,Jaeger C,Wassmuth R.A TaqI polymorphism at the ovine β_A-inhibin (INHBA)locus[J].Animal Genetics,1992,23(3):291.
157.Hiendleder S,Jaeger C,Leyhe B,Wassmuth R.Mendelian inheritance of a Taq I polymorphism at the ovine β_B-inhibin(INHBB)locus[J].Journal of Animal Breeding and Genetics,1992b,109:320.
158.Jaeger C,Hiendleder S.Cosmid cloning and characterization of the coding regions and regulatory elements of the ovine α-(INHA),β_A-(INHBA)and β_B-inhibin(INHBB)genes[J].Animal Genetics,1994,25(Suppl.2):33.
159.Leyhe B,Hiendleder S,Jaeger C,Wassmuth R.Pronounced differences in the frequency of Taq Ⅰ β_A-inhibin alleles between sheep breeds with different reproductive performance[J].Animal Genetics,1994,25(1):41-43.
160.Jager C,Hiendleder S.Genomic cloning and sequence analysis of three different ovine β_B-inhibin(INHBB)alleles[J].Animal Genetics,1996,27(Suppl.2):92.
161.泽格·欧特,著.杨达,译.人类遗传连锁分析[M].北京:北京农业大学出版社.1995.15-16.
162.Hiendleder S,Lewalski H,Jaeger C,Plante Y.Genomic cloning and comparative sequence analysis of different alleles of the ovine β_A-inhibin/activin(INHBA)gene as a potential QTL for litter size[J].Animal Genetics,1996,27(Suppl.2):119.
163.Hiendleder S,Leyhe B,Jaeger C,Erhardt G,Wassmuth R.Molecular characterization of ovine α-,β_A-and β_B-inhibin/activin alleles[J].Journal of Animal Breeding and Genetics,1996,113:363-372.
2.Piper L R,Bindon B M,Nethery R D.The Booroola Merino,Melbourne:CSIRO Division of Animal production[C],1982,9-20.
3.Bodin L,Elsen J M,Poivey J P,San Cristobal-Gaudy M,Belloc J P,Eychenne F.Hyper prolificacy in the French La caune sheep breed-A possible major gene[C].In:Laurie Piper ed.Proceedings of 6 WCGALP.NSW Australia:University of New England,1998,27:11-14.
4.施启顺.畜禽主基因研究进展[J].湖南农业大学学报,1999,25(1):81-86.
5.陈红菊,岳永生.动物主基因的研究进展[J].山东备牧兽医,2001(6):35-37.
6.曹果清,周忠孝,袁建霞,朱向芳.猪主要经济性状候选基因的研究进展,遗传,2002,24(2):214-218.
7.柴丽娟,赵先萍,刘文忠.绵羊主基因的研究进展[J].中国畜牧杂志,2003,39(3):51-52.
8.徐华,孙少华.畜禽主基因的研究现状与检测方法[J].当代畜牧,2004,2:46-49.
9.耿荣庆,王兰萍.我国绵羊多胎性能的研究与利用概况[J].畜禽业,2001(5):6-7.
10.耿荣庆.我国绵羊多胎性能主基因的开发利用[J].河南畜牧兽医,2001,22(1):13-13.
11.薛慧良,周忠孝.绵羊繁殖性状候选基因的研究进展[J].动物科学与动物医学,2003,93(3):32-35.
12.张秀华,吴登俊.绵羊繁殖性状的研究进展[J].草食家畜,2003(1):19-21.
13.季从亮,储明星,陈国宏,周国利,朱颜.4个绵羊品种褪黑激素受体1a基因第二外显子PCR-RFLP分析[J].华中农业大学学报,2003,22(2):105-109.
14.李碧侠,储明星,王金玉.绵羊GDF9基因PCR-SSCP分析[J].遗传学报,2003,30(4):307-310.
15.Chu M X,Ji C L,Chen G H.Association between PCR-RFLP of melatonin receptor 1a gene and high prolificacy in Small Tail Han sheep[J].Asian-Aust J Anim Sci,2003,16(12):1701-1704.
16.Guo W,Chu M X,Deng X M,Feng J D,Li Ning,Wu Changxin.Association of a single codon deletion in bone morphogenetic protein 15 gene with prolificacy in Small Tail Han sheep[J].Asian-A ust.J Anim Sci,2004,17(11):1491-1495.
17.朱靖,杜立新.小尾寒羊多胎性候选基因的研究进展[J].动物科学与动物医学2004,21(9):16-18.
18.储明星.利用分子育种技术选育肉用多胎绵羊新品系[J].中国农业科技导报,2004,6(6):15-19.
19.Chu M X,Li B X,Wang J Y,Ye S C,Fang L.Association between PCR-SSCP of growth differentiation factor 9 gene and high prolificacy in Small Tail Han sheep[J].Anita Biotechnol,2004,15(2):111-120.
20.Yan Y D,Chu M X,Zeng Y Q,Fang L,Ye S C,Wang L M,Guo Q K,Han D Q,Zhang Z X,Wang X J,Zhang X Z.Bone morphogenetie protein receptor IB as a candidate gene for prolificacy in Small Tail Han and Hu ewes[J].Chinese Journal of Agricultural Biotechnology,2005,2(2):125-130.
21.储明星,桑林华,王金玉,方丽,叶素成.小尾寒羊高繁殖力候选基冈BMPl5和GDF9的研究[J].遗传学报,2005,32(1):38-45.
22.毕晓丹,储明星,金海国,方丽,叶素成.小尾寒羊高繁殖力候选基因ESR的研究[J].遗传学报,2005,32(10):1060-1065.
23.闫亚东,储明星,曾勇庆,方丽,叶素成,王利民,国庆坤,韩代勤,张兆新,王西筠,张新珍.小尾寒羊和湖羊高繁殖力候选基因BMPR-IB的研究[J].农业生物技术学报,2005,13(1):66-71.
24.储明星,何远清,王金玉,Olowofeso O,叶素成,方丽.绵羊肌细胞生成素基因外显子1的PCR-SSCP分析[J].农业生物技术学报,2005,13(1):77-80.
25.储明星,成荣,陈国宏,方丽,叶素成.小尾寒羊和湖羊高繁殖力候选基因BMP15的研究[J].安徽农业大学学报,2005,32(3):278-282.
26.艾君涛,管峰,杨利国.绵羊繁殖性状主基因研究进展[J].中国农学通报,2005,21(6):39-41
27.Dube J L,Wang P,Elvin J.The bone morphogenetic protein15 gene is linked and expressed in oocytes[J].Molecular Endocrinology,1998,12(2):1809-1817.
28.Otsuka F.Bone morphogenetic protein 15 inhibits follicle stimulating hormone(FSH)action by suppressing FSH receptor expression[J].Biol Chem,2001,276(14):1187-1192.
29.Galloway S M,McNatty K P,Cambridge L M.Mutations in an oocyte derived growth diferentiation factor gene(BMPI5)cause increased ovulation rate and infertility in a dosage-sensitive manner[J].Nature Genetics,2000,25(3):279-283.
30.Yan C N,Wang P,DeMayo J,Demayo F J,Elvin J A,Carino C,Prasad S V,Skinner S S,Dunbar B S,Dube L J,Celeste A J,Matzuk M M.Synergistic roles of bone morphogenetic proein 15 and growth differentiation factor 9 in ovarian function[J].Mol Endocrinol,2001,15(6):854-866.
31.Juengel J L,Hudson N L,Heath D A,Smith P,Reader K L,Lawrence S B,O'Connell A R,Laitinen M P,Cranfield M,Groome N P,Ritvos O,McNatty K P.Growth differentiation factor 9 and bone morphogenetic protein 15 are essential for ovarian follicular development in sheep [J].Biol Reprod,2002,67(6):1777-1789.
32.Hanrahan J P,Gregan S M,Mulsant P,Muilen M,Davis G H,Powell R,Galloway S M.Mutations in the genes for oocyte-derived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in Cambridge and Belclare sheep(Ovis aries)[J].Biol Reprod,2004,70(4):900-909.
33.何远清,储明星,王金玉,方丽,叶素成.6个山羊品种高繁殖力候选基因BMP15多态性研究.安徽农业大学学报[J].2006,33(1):61-64.
34.吴泽辉,储明星,李学伟.生长分化因子9基因及其在生殖中的作用[J].遗传,2005,27(3):481-486.
35.Kaivo-Oja N,Bondestam J,Kamarainen M,Koskimies J,Vitt U,Cranfield M,Vuojolainen K,Kallio J P,Olkkonen V M,Hayashi M,Moustakas A,Groome N P,ten Dijke P,Hsueh A J,Ritvos O.Growth differentiation factor-9 induces Smad2 activation and inhibin B production in human granulose-luteal cells[J].J Clin Endocrinol Metab,2003,88(2):755-762.
36.Dong J W,Albertini D F,Nishimori K,Kumar T R,Lu N,Matzuk M M.Growth differentiation factor 9 is required during early ovarian folliculogenesis[J].Nature,1996,383(6600):531-535.
37.Bodensteiner K J,Clay C M,Moeller C L,Sawyer H R.Molecular cloning of the ovine growth/differentiation factor-9 gene and expression of growth/differentiation factor-9 in ovine and bovine ovaries[J].Biol Reprod,1999,60(2):381-386.
38.Juengel J L,Hudson N L,Heath D A,Smith P,Reader K L,Lawrence S B,O'Connell A R,Laitinen M P,Cranfield M,Groome N P,Ritvos O,McNatty K P.Growth differentiation factor 9 and bone morphogenetic protein 15 are essential for ovarian follicular development in sheep [J].Biol Reprod,2002,67(6):1777-1789.
39.Juengel J L,Hudson N L,Whiting L,McNatty K P.Effects of immunization against bone morphogenetic protein 15 and growth differentiation factor 9 on ovulation rate,fertilization,and pregnancy in ewes[J].Biol Reprod,2004,70(3):557-561.
40.Mc Pherron A C,Lee S J.GDF-3 and GDF-9:two new members of the transforming growth factor-beta superfamily containing a novel pattern of cysteines[J].J Biol Chem,1993,268(5):3444-3449.
41.Incerti B,Dong J,Borsani G,Matzuk M M.Structure of the mouse growth-differentiation factor 9 gene[J].Biochem Biophys Acta,1994,1222(1):125-128.
42.杨晶.小尾寒羊GDF9基因和BMP15基因多态性及其与高繁殖力关系的研究.中国农业科学院硕士论文[D],2006.6.
43.何远清,储明星,王金玉,方丽,叶素成.山羊高繁殖力候选基冈GDF9的RFLP分忻[J].农业生物技术学报,2006,14(1):135-136.
44.吴泽辉,储明星,李学伟,方丽,叶素成,刘忠慧,陈国宏.山羊生长分化因子9基因外显子2的PCR-SSCP分析[J].中国农业科学,2006,39(4):802-808.
45.WolfJ B,David V A,Deutch A H.Identification of a distal regulatory element in the 5' flanking region of the bovine prolactin gene[J].Nucleic Acids Res,1990,18(16):4905-44912.
46.Kansaku N.Genetic variation of chicken prolactin promoter[M].Published Only in DataBase (2000).
47.崔建勋,梁勇,邓学梅,张豪,杨关福,张细权.鸡催乳素基因5'侧翼调控区近端启动子序列多态性及其与产蛋性能的关系[C].第十一次全国家禽学术讨论会论文集,长春:吉林科学技术出版社.2003,33-36.
48.Zhang H M,Denise S K,Ax R L.Diallielic single-stranded conformational polymorphism detected in the bovine prolactin gene[J].J Anim Sci,1994,72(1):256-257.
49.Vincent A L,Rothschild M E A restriction fragment length polymorphism in the ovine prolactin gene[J].J Anim Sci,1997,75(6):1686.
50.储明星.猪产仔数性状候选基因的研究进展[J].畜牧与兽医,2001,33(1):36-37.
51.阮征,凌家瑞.以催乳素受体基因(PRLR)作为母猪产仔数候选基因的分析[J].上海畜牧兽医通讯2002(1):20-21.
52.李国治,连林生,鲁绍雄,严达伟.以催乳素受体基因作为撒坝猪部分生产性能候选基因 的研究[J].养猪,2005(4):36-38.
53.张冬杰,沙伟,杨国伟,刘娣.催乳素受体基因(PRLR)与产仔数关系的研究[J].安徽农业科学,2005,33(12):2340-2341.
54.曹果清,李步高,刘建华,杨文平.猪催乳素受体(PRLR)基冈Alu Ⅰ多态性与产仔数关系的研究[J].山西农业大学学报,2004,24(3):253-255.
55.银晶.山羊催乳素受体基因内含子Ⅰ和Ⅱ单链构象多态性与产羔性能关系的研究.山西农业大学硕士学位论文[D].2004.12.
56.Reppert S M,Weaver D R,Ebisawa T.Cloning and characterization of a mammalian receptor that mediates reproductive and circadian responses[J].Neuron,1994,13(5):1177-1185.
57.Skinner T M,Lopez-Corrales N L,Anderson S I.Genetic and physical mapping of the porcine melatonin receptorlB gene(MTNRIB)to chromosome 9[J].Cytogenet Cell Genet,2000,90(2):53-55.
58.Slaugenhaupt S A,Roca A L,Liebert C B,Aitherr M R,Gusella J F,Reppert S M.Mapping of the gene for the Mella-melatonin receptor to human chromosome 4(MTNRIA)and mouse chromosome 8(Mtnrla)[J].Genomics,1995,27(2):355-357.
59.Messer L A,Wang L Z,Tuggle C K.Mapping of the melatonin receptor la(MTNR1A)gene in pigs,sheep,and cattle[J].Mammalian Genome,1997,8(5):368-370.
60.Pelletier J,Bodin L,Hanocq E,Malpaux B,Teyssier J,Thimonier J,Chemineau P.Association between expression of reproductive seasonality and alleles of the gene for Mella receptor in the ewe[J].Biol Reprod,2000,62(4):1096-1101.
61.季从亮,储明星,陈国宏.4个绵羊品种褪黑激素受体1a基因底二外显子PCR-RFLP分析[J].华中农业大学学报,2003,22(2):105-109.
62.Migaud M,Gavet S,Pelletier J.Partial cloning and polymorphism of the melatoninla(Mella)receptor gene in two breeds of goat with different reproductive seasonality[J].Repro-dution,2002,124(1):59-64.
63.Buger H G.lnhibin:definition and nomenclature including related substances[J].J Endocrinol,1988,117(2):159-160.
64.Leversha LJ,Robertson D M,de Vos F L,Morgan F J,Hearn M T,Wettenhall RE,Findlay JK,Burger HG,de Kretser D M.Isolation of inhibin from ovine follicular fluid.J Endocrinol.1987May;113(2):213-221.
65.路长林.神经肽基础与临床.重庆[M]:第三军医大学出版社,2000.
66.Vale W,Hsueh A,Rivier C,Yu J.The inhibin/activin family of hormones and growth factors,in Handbook of experimental pharmacology:peptide growth factors and their receptor Ⅱ Sporn[J].edvol.1990,95:211-248.
67.Ying S Y.lnhibins and activins,chemical properties and biological activity.Proc Soc Exp Biol Med.1987,186(3):253-264.
68.Ying S Y.Inhibins,activins,and follistatins:gonadal proteins modulating the secretion of follicle-stimulating hormone[J].Endocr Rev,1988,9(2):267-293.
69.Findlay J K.An update on the roles of inhibin,activins and follistain as local regular of folliculogenesis[J].Bio Reprod,1993,48(1):15-23.
70. Mather J P, Woodruff T K. Paracrine regular on reproductive function by inhibin and activin[J].Pro Sci Exp Biol Med, 1992,201(1):1-3.
71. Adams M H, Baker C B, McDowell K J. Molecular cloning and sequencing of equine inhibin alpha cDNA[J].Animal Biotechnology, 1996,7(1):1-9.
72 Hiendleder S, Lewalski H, Jaeger C, Pracht P, Erhardt G. Nucleotide sequence of ovine α-inhibin (INHA) genes and evaluation of RFLP marker effects on reproductive performance[J].Animal Genetics, 1996, 27(Suppl.2):91-92.
73. Hiendleder S, Lewalski H, Jaeger C, Plante Y. Genomic cloning and comparative sequence analysis of different alleles of the ovine β_A- inhibin/activin (INHBA) gene as a potential QTL for litter size[J]. Animal Genetics, 1996,27(Suppl.2):119.
74. Forage R G, Ring J M, Brown R W. Cloning and sequence analysis of cDNA species coding for the two subunits of inhibin from bovine follicular fluid[J]. Proc Natl Acad Sci U S A, 1986,83(10): 3091-3095.
75. Rodgers R J. Cloning of the inhibin/activin beta B subunit gene from the Booroola merino sheep[J]. J Mol Endocrinol, 1991,6(1):87-93.
76. Mason A J, Hayflick J S, Ling N. Complementary DNA sequences of ovarian follicular fluid inhibin show precursor structure and homology with transforming growth factor- beta[J].Nature, 1985, 318(6047):659-663.
77. Mason A J, Niafl H D, Seeburg P H. Structure of two human ovarian inhibins[J]. Biochem Biophys Res Commun,1986,135:957-964
78. Woodruff T K, Meunier H, Jones P B. Rat inhibin: molecular cloning of alpha- and beta-subunit complementary deoxyribonucleic acids and expression in the ovary[J]. Mol Endocrinol,1987, 1(8):561-568.
79. Barton D E, Yang-Feng T L, Mason A J, Seeburg P H, Francke U. Mapping of genes for inhibin subunits α, β_A and β_B on human and mouse chromosomes and studies of jsd mice[J]. Genomics.1989,5(1):91-99.
80. Lafuse W P, Zwilling B S. Localization of the inhibin β_B gene on mouse chromosome 1[J].Mammalian Genome, 1993,4(7):399—400.
81. Neibergs H L, Gallagher D S, Georges M, Sargeant L S, Dietz A B, Womack J E. Physical mapping of inhibin β_A in domestic cattle[J]. Mammalian Genome, 1993,4(6):328—332.
82 Brunner R M, Goldammer T, Hiendleder S, Jager C, Schwerin M. Mapping of the gene encoding for inhibin-βA (INHBA) to chromosome 4q26 in sheep[J]. Mammalian Genome,1995, 6(4):308-309.
83. Brunner R M, Goldammer T, Hiendleder S, Jager C, Schwerin M. Comparative mapping of the gene coding for inhibin-α (INHA) to chromosome 2 in sheep and cattle[J]. Mammalian Genome, 1995, 6(9):685-686.
84. Goldammer T, Brunner R M, Hiendleder S, Schwerin M. Comparative mapping of sheep inhibin subunit β_b to chromosome 2 in sheep and cattle by fluorescence in situ -hybridization[J]. Animal Genetics, 1995, 26(3): 199-200.
85. Goldammer T, Brunner R M, Hiendleder S, Schwerin M. Comparative mapping of sheep inhibin subunits α (INHA) and β_B (INHBB) to chromosome 2 in goat by FISH[J]. Mammalian Genome, 1995, 6(9):685-686.
86. Hiendleder S, Leyhe B, Jaeger C,Erhardt G and Wassmuth R. Molecular characterization of ovine α-, β_A- and β_B-inhibin/activin alleles[J]. Journal of Animal Breeding and Genetics, 1996,113:363-372.
87. Roberts V J, Sawchenko P E, Vale W. Expression of inhibin/activin subunit messenger ribonucleic acids during rat embryogenesis[J]. Endocrinology, 1991,128(6):3122—3129.
88. Roberts V J, Barth S, el-Roeiy A, Yen S S. Expression of inhibin/activin system messenger ribonucleic acids and proteins in ovarian follicles from women with polycystic ovarian syndrome[J]. J Clin Endocrinol Metab, 1994,79(5):1434-1439.
89. Drummond A E, Dyson M, Mercer J E, Findlay J K. Differential responses of post-natal rat ovarian cells to FSH and activin[J]. Mol Cell Endocrinol, 1996,122(1):21 -32.
90. Drummond A E, Dyson M, Thean E, Groome N P, Robertson D M, Findlay J K. Temporal and hormonal regulation of inhibin protein and subunit mRNA expression by post-natal and immature rat ovaries[J]. J Endocrinol, 2000,166(2):339-354.
91. Engelhardt H, Harkness L M, Thomas G B, Brooks A N, McNeilly A S, Baird D T. Expression of inhibin alpha- and beta A-subunit mRNA and protein in the fetal sheep ovary throughout gestation[J]. Mol Cell Endocrinol, 1995,107(2): 141-147.
92. Nambo Y, Nagata S, Oikawa M, Yoshihara T, Tsunoda N, Kohsaka T, Taniyama H, Watanabe G,Taya K. High concentrations of immunoreactive inhibin in the plasma of mares and fetal gonads during the second half of pregnancy[J]. Reprod Fertil Dev, 1996, 8(8): 1137— 1145.
93. Torney A H, Robertson D M, Hodgson Y M, de Kretser D M. In vitro bioactive and immunoactive inhibin concentrations in bovine fetal ovaries and testes throughout gestation[J].Endocrinology, 1990,127(6):2938-2946.
94. Chen C C, Johnson P A. Expression of inhibin alpha and inhibin/activin beta A subunits in the granulosa layer of the large preovulatory follicles of the hen[J]. Biol Reprod, 1996, 55(2):450-454.
95. Fleming J S, Galloway S M, Crawford R J, Tisdall D J, Greenwood P J. Tissue-specific variation in the length of the 5' untranslated region of the beta A-inhibin mRNA in sheep[J].Mol Reprod Dev, 1995, 40(1):1-8.
96. Bernard D J, ChapmanS C, Woodruff T K. Mechanisms of inhibin signal transduction[J].Recent Prog Horm Res, 2001, 56:417-450.
97. Eramaa M, Ritvos O. Transforming growth factor-beta 1 and -beta 2 induce inhibin and activin beta B-subunit messenger ribonucleic acid levels in cultured human granulosa-luteal cells[J].Fertil Steril, 1996,65(5): 954-960.
98. Kubo T, Shimasaki S, Kim H, Li D, Erickson G F. Activin-induced inhibin alpha-subunit production by rat granulosa cells requires endogenous insulin-like growth factor-I [J]. Biol Reprod, 1998,58(3):712-718.
99. Tamura K, Hatsuta M, Watanabe G, Taya K, Kogo H. Inhibitory regulation of inhibin gene expression by thyroid hormone during ovarian development in immature rats[J]. Biochem Biophys Res Commun, 1998, 242(1):102-108.
100. Debieve F, Thomas K. Control of the human inhibin alpha chain promoter in cytotrophoblast cells differentiating into syncytium[J]. Mol Hum Reprod,2002, 8(3):262-270.
101. Smyth C D, Gosden R G,McNeillyA S. Effect of inhibin immunoneutralization on steroidogenesis in ratovarian follicles in vitro[J]. Endocrinol, 1994, 140(3): 437—443.
102 Ying S Y, Czvik J, Becker A. Secretion of follicle-stim-ulating horm one and production of inhibin are reciprocally related[J]. Proc Natl Acad Sci, U S A, 1 987,84(13):4631 —4635.
103. Hillier S G, Yong E L, Illingworth P J. Effect of recombinant inhibin on androgen syn thesis in cultured human thecacells[J]. Mol Cell Endocrinol,1991,75(2):R(?)—6.
104. Tannetta D S, Feist S A, Bleach E C, Groome N P, Evans L W, Knight P G. Effects of active immunization of sheep against an amino terminal peptide of the inhibin alpha C subunit on intrafollicular levels of activin A,inhibin A and follistatin[J].J Endocrinol,1998,157(1):157-168.
105.张德坤.应用抑制素基因免疫技术诱导单胎绵羊孪生及其影响因素与作用机制研究[D].南京农业大学博士论文,2004
106.姜勋平,杨利国,刘桂琼.抑制素基因免疫对小鼠生殖的影响[J].中国兽医学报,2002,22(4):368-369.
107.周虚,田谷一善.抑制素被动免疫对黄体中期山羊生殖激素分泌的影响[J].中国兽医学报,2002,22(4):370-372.
108.Price C A,Morals B A,Oshea T,Webb R.Active immunization of cattle against partially purified follicular fluid from sheep[J].J Reprod Fert,1987,81(1):161-168.
109.Takedomi T,Kishi H,Medan M S,Aoyagi Y,Konishi M,Itoh T,Yazawa S,Watanabe G,Taya K.Active immunization against inhibin improves superovulatory response to exogenous FSH in cattle[J].J Reprod Dev,2005,51(3):341-346.
110.Medan M S,Akagi S,Kaneko H,Watanabe G,Tsonis C G,Taya K.Effects of re-immunization of heifers against inhibin on hormonal profiles and ovulation rate[J].Reproduction,2004,128(4):475-482
111.Schanbacher B D,Schemm S R,Rhind S M.Gonadotrophin concentrations and ovulation rates in Suffolk ewes actively of passively immunized against inhibin alpha[J].Reprod Fert,1991,93(1):133-139.
112.Medan M S,Takedomi T,Aoyagi Y,Konishi M,Yazawa S,Watanabe G,Taya K.The effect of active immunization agaist inhibin on Gonadotropin secretions and follicular dynamics during the estrous cycle in cows[J].J Reprod Dev,2006,52(1):107-113.
113.Brown R W,Hungerford J W,Greenword P E,Bloor R J,Evans D F,Tsonis C G,Forage R G.Immunization against recombinant bovine inhibin a subunit causes increased ovulation rates in gilts[J].Reprod Fert,1990,90(1):199-205.
114.Wheaton J E,Meyer R L,Jones R H,Kramer A J.Effects of passive immunization using antibody against an aipha-inhibin petptide on follicle-stimulating hormone concentrations and litter size in sows[J].Therogenology,1998,49(4):813-822.
115.Alabart JL,Folch J,Cocero MJ,Cognie Y.Effect of immunization on reproducvive performance,embryo quality and progesterone in Rasa Aragonesa ewes actively immunized against androstenedione or passively immunized against testosterone.Theriogenology.1991,35(4):799-813.
116.Medan M S,Watanabe G,Nagura Y,Kanazawa H,Fujita M,Taya K.Passive immunoneutralization of endogenous inhibin increase ovulation rate in miniature Shiba goats[J].J Reprod Dev,2004,50(6):705-710.
117.华进联.抑制素免疫对羊生殖的影响[J].畜牧兽医杂志,2000,19(3):15-17.
118.D'Alessandro A,Martemucci G,Iaffaldano N.Active immunization with a synthetic fragment of pig inhibin alpha-subunit increases ovulation rate and embryo production in superovulated ewes but season affects its efficiency[J].J Reprod Fertil,1999,115(1):185-191.
119.王杏龙,Wolfgang Holtz.抑制素免疫山羊卵巢对外源性促性腺激素的反应[J].中国备牧 杂志,2003,39(1):3-4.
120.刘娜.抑制素免疫提高家畜的排卵率[J].畜牧兽医杂志,1996,15:92-93.
121.Lovell T M,Knight P G,Groome N P,Gladwell R T.Changes in plasma inhibin A level during sexual maturation in the female chicken and the effects of active immunization against inhibin alpha-subunit on reproductive hormone profiles and ovarian function[J].Biol Reprod,2001,64(1):188-196.
122.Ahn J,You S,Kim H,Chaiseha Y,El Halawani M.Effects of active immunization with inhibin alpha subunit on reproductive characteristics of turkey hens[J].Biol Rerod,2001,65(5):1594-1600.
123.王进荣,杨利国,王元兴.人抑制家主动免疫对母鸡产蛋和内分泌的影响[J].中国畜牧杂志,2001,37(2):9-11.
124.Larsen N J,Nielsen V H.Apa I and Cfo I polymorphisms in the porcine growth hormone gene[J].Animal Genetics,1993,24(1):71
125.Neyroud N,Denjoy I,Donger C,Gary F,Villain E,Leenhardt A,Benali K,Schwartz K,Coumel P,Guicheney P.Heterozygous mutation in the pore of potassium channel gene KvLQT1 causes an apparently normal phenotype in long QT syndrome[J].Eur J Hum Genet,1998,6(2):129-133.
126.方美英,姜志华,刘红林,黄月英,李齐贤,朱婉茹,李学斌,陈杰.应用 PCR-RFLP、PCR-SSCP检测猪的氟烷基因[J].养猪,]997,8(1):29-29.
127.刘佳建,李奎,彭中镇.甘油浓度对PCR-SSCP分辨效果的影响[J].生物技术通讯,1998,6(2):160-161
128.姜运良,李宁.小片段DNA未知SNPs的PCR-SSCP检测方法分析[J].中国生物化学与分子生物学报2002,18(6):674-678..
129.Yao J B,Aggrey S E,Zadwomy D,Hayes J F,Kuhnlein U.Sequence variations in the bovine growth hormone gene characterized by sing-strand conformation polymorphism(sscp)analysis and their association with milk production traits in Holsteins[J].Genetics,1996,144(4):1809-1816.
130.Marklund S,Chaudhary R,Marklund L,Sandberg K,Andersson L.Extensive mtDNA diversity in horses revealed by PCR-SSCP analysis[J].Anim Genet,1995,26(3):193-196.
131.Maddox R Z.Amale linkage map of the cattle(Bos Taurus)genome[J].Journal of Heredity,1996,87:261-271.
132.赵兴波.储明星.绵羊线粒体DNA控制区5'端序列PCR-SSCP与序列分析[J].遗传学报,2001,28(3):225-228.
133.李碧侠,储明星,王金玉.绵羊GDF9基因PCR-SSCP分析[J].遗传学报,2003,30(4):307-310.
134.闫亚东,储明星,曾勇庆,方丽,叶素成,王利民,国庆坤,韩代勤,张兆新,王西筠,张新珍.小尾寒羊和湖羊高繁殖力候选基因BMPR-IB的研究[J].农业生物技术学报,2005,13(1):66-71.
135.储明星,何远清,王金玉,Olowofeso Olajide,叶素成,方丽.绵羊肌细胞生成素基因外显子1的PCR-SSCP分析[J].农业生物技术学报,2005,13(1):77-80.
136.中国农业信息网数据资料.http://www.agri.gov.cn/sjzl/
137.1988,88-90,98-100.《中国羊品种志》编写组.中国羊品种志.上海:上海科学技术出版社,1988,88-90,98-100.
138.Roberts A J,Reeves J J.Kidding rates of Angora goats passively immunized against estrogens [J].J Anim Sci,1988,66(10):2443-2447.
139.Thompson D A,Cronin C N,Martin F.Genomic cloning and sequence analyses of the bovine alpha-,beta A- and beta B-inhibin/activin genes.Identification of transcription factor AP-2-binding sites in the 5'-flanking regions by DNase I footprinting[J].Eur J Biochem,1994,226(3):751-764.
140.Don R H,Cox P T,Wainwright B J,Baker K,Mattick J S.Touchdown'PCR to circumvent spurious priming during gene amplification[J].Nucleic Acids Res,1991,19(14):4008.
141.Bostin D,White R L,Skolnick M,Davis R W.Construction of a genetic linkage map in man using restriction fragment length polymouphism[J].American Journal of Human Genetic,1980,32:314-331.
142.Weir B S,Cockerham C C.Estimating F-statistics for the analysis of population structure[J].Evolution,2002,36:721-750.
143.Hayashi K.PCR-SSCP:A mothed for detection of mutation.GATA,1992,9(3):73-79.
144.卢圣栋.现代分子生物学试验技术[M].北京:高等教育出版社,1993,95-103.
145.张学,孙开来.PCR-SSCP分析技术及其应用[J].国外医学遗传学分册,1992,5(5):225-231.
146.根井正利 著.王家玉,译.分子群体遗传学与进化论[M].北京:北京农业出版社.1985:15-154.
147.常洪.家畜遗传资源纲要[M].北京:中国农业出版社.1995:28-135.
148.刘文忠,张沅.安哥拉山羊育种和生产中的非遗传因素[J].中国草食动物,2004,24(1):46-48.
149.刘文忠,张沅.安哥拉山羊的遗传改良[J].中国畜牧兽医,2004,31(3):27-30
150.Nei Masatoshi.Molecular population genetic and evolution[M].Beijing.Agricultural Publishing House.1985,15-154.
151.欧刚叙向,邓灶福,黄生强.微卫星标记BM143在4个山羊品种中的遗传多样性分析[J].经济动物学报,2005,9(2):103-106
152.侯冠或,马月辉,王东劲.6个绵羊群体遗传分化研究[J].热带农业科学,2005,25(5):33-35.
153.杨章平,常洪,孙伟.结构基因座和微卫星标记应用于绵(山)羊群体遗传分化的比较研究[J].畜牧兽医学报,2003,34(5):427-433
154.张增利,巩元芳,刘铮铸.我国主要地方绵羊品种遗传亲缘关系[J].中国兽医学报,2004,24(5):508-511.
155.汪志国,杨章平,马月辉,王庆华,毛永江,常洪,周群兰,许明.长江中下游以及东南沿海的7个山羊群体的遗传多样性分析[J].畜牧兽医学报,2006,37(1),1-6.
156.Hiendleder S,Leyhe B,Jaeger C,Wassmuth R.A TaqI polymorphism at the ovine β_A-inhibin (INHBA)locus[J].Animal Genetics,1992,23(3):291.
157.Hiendleder S,Jaeger C,Leyhe B,Wassmuth R.Mendelian inheritance of a Taq I polymorphism at the ovine β_B-inhibin(INHBB)locus[J].Journal of Animal Breeding and Genetics,1992b,109:320.
158.Jaeger C,Hiendleder S.Cosmid cloning and characterization of the coding regions and regulatory elements of the ovine α-(INHA),β_A-(INHBA)and β_B-inhibin(INHBB)genes[J].Animal Genetics,1994,25(Suppl.2):33.
159.Leyhe B,Hiendleder S,Jaeger C,Wassmuth R.Pronounced differences in the frequency of Taq Ⅰ β_A-inhibin alleles between sheep breeds with different reproductive performance[J].Animal Genetics,1994,25(1):41-43.
160.Jager C,Hiendleder S.Genomic cloning and sequence analysis of three different ovine β_B-inhibin(INHBB)alleles[J].Animal Genetics,1996,27(Suppl.2):92.
161.泽格·欧特,著.杨达,译.人类遗传连锁分析[M].北京:北京农业大学出版社.1995.15-16.
162.Hiendleder S,Lewalski H,Jaeger C,Plante Y.Genomic cloning and comparative sequence analysis of different alleles of the ovine β_A-inhibin/activin(INHBA)gene as a potential QTL for litter size[J].Animal Genetics,1996,27(Suppl.2):119.
163.Hiendleder S,Leyhe B,Jaeger C,Erhardt G,Wassmuth R.Molecular characterization of ovine α-,β_A-and β_B-inhibin/activin alleles[J].Journal of Animal Breeding and Genetics,1996,113:363-372.