绵羊BMP15和BMPR-IB基因多态性研究
摘要
本研究以骨形态发生蛋白15(BMP15)和骨形态发生蛋白受体(BMPR-IB)为候选基因,以内蒙古地方品种蒙古羊、内蒙古细毛羊、呼伦贝尔羊、中国美利奴羊(科尔沁型)为研究对象,对在生殖中起重要作用的BMP15基因和BMPR-IB基因进行多态性检测,以比较它们在不同绵羊品种中的多态性,旨在寻找与繁殖力相关的遗传标记,为内蒙古绵羊选育和改良提供理论依据。
(1)通过对GenBank中绵羊BMP15基因作比对分析,在其外显子1序列内设计一对引物,采用PCR技术扩增其部分序列并进行PCR-SSCP多态性分析。实验结果在4个绵羊品种中均检测到BMP15的突变,且检测到的BMP15的突变在4个绵羊品种中均呈现三种基因型。分析实验结果显示:BMP15基因外显子1中虽然存在突变位点,但这一突变位点对绵羊繁殖力高低没有显著影响。
(2)利用GenBank中绵羊的BMPR-IB序列信息,通过序列比对、分析,采用PCR技术扩增其部分序列并进行PCR-RFLP多态性分析。实验结果表明:繁殖力较高的中国美利奴羊(科尔沁型)BMPR-IB基因编码序列第746位碱基处发生了与Booroola Merino绵羊相同的突变(A746G),而繁殖力较低的蒙古羊、呼伦贝尔羊、内蒙古细毛羊、则没有发生这种突变。
Abstract BMP15 and BMPR-IB as candidate genes on the fecundity,The polymorphism of BMP15 gene and BMPR-IB gene were detected in four sheep breeds mongolian sheep、hu lun bei er sheep、Inner mongolian fine wool sheep and china merino( ke er qin type). in order to find a molecular genetic marker which has significance in mongolian sheep breeding.
(1) the primer was designed by BMP15 exon1 sequence in the GenBank, adopted PCR technique to magnify the sequence and analysis the polymorphism by PCR-SSCP of BMP15 gene in different four breeds of sheep.The results were as follows:The polymorphisms of PCR-SSCP existed in the four ewe brains,and this polymorphism show three gene types in every one brain.AA、A+、++ type;The mutation detected did not affect significantly the prolificacy of ewes.
(2) the primer was designed by BMPR-IB sequence in the GenBank, adopted PCR technique to magnify the sequence and analysis the polymorphism by PCR-RFLP of BMPR-IB gene in different breeds of sheep. The results in dicated that there was a same mutation (A746G) of BMPR-IB gene in china Merino as that in Booroola Merino ewes. That Mutation did not exist in mongolian sheep、hu lun bei er sheep、Inner mongolian fine wool sheep.
(1)通过对GenBank中绵羊BMP15基因作比对分析,在其外显子1序列内设计一对引物,采用PCR技术扩增其部分序列并进行PCR-SSCP多态性分析。实验结果在4个绵羊品种中均检测到BMP15的突变,且检测到的BMP15的突变在4个绵羊品种中均呈现三种基因型。分析实验结果显示:BMP15基因外显子1中虽然存在突变位点,但这一突变位点对绵羊繁殖力高低没有显著影响。
(2)利用GenBank中绵羊的BMPR-IB序列信息,通过序列比对、分析,采用PCR技术扩增其部分序列并进行PCR-RFLP多态性分析。实验结果表明:繁殖力较高的中国美利奴羊(科尔沁型)BMPR-IB基因编码序列第746位碱基处发生了与Booroola Merino绵羊相同的突变(A746G),而繁殖力较低的蒙古羊、呼伦贝尔羊、内蒙古细毛羊、则没有发生这种突变。
Abstract BMP15 and BMPR-IB as candidate genes on the fecundity,The polymorphism of BMP15 gene and BMPR-IB gene were detected in four sheep breeds mongolian sheep、hu lun bei er sheep、Inner mongolian fine wool sheep and china merino( ke er qin type). in order to find a molecular genetic marker which has significance in mongolian sheep breeding.
(1) the primer was designed by BMP15 exon1 sequence in the GenBank, adopted PCR technique to magnify the sequence and analysis the polymorphism by PCR-SSCP of BMP15 gene in different four breeds of sheep.The results were as follows:The polymorphisms of PCR-SSCP existed in the four ewe brains,and this polymorphism show three gene types in every one brain.AA、A+、++ type;The mutation detected did not affect significantly the prolificacy of ewes.
(2) the primer was designed by BMPR-IB sequence in the GenBank, adopted PCR technique to magnify the sequence and analysis the polymorphism by PCR-RFLP of BMPR-IB gene in different breeds of sheep. The results in dicated that there was a same mutation (A746G) of BMPR-IB gene in china Merino as that in Booroola Merino ewes. That Mutation did not exist in mongolian sheep、hu lun bei er sheep、Inner mongolian fine wool sheep.
引文
1 Jennifer L, Dube M,Wang P, et al. The bone morphogenetic protein 15 gene is X-linked and expressed in oocytes [J]. Mole Endor,1998,12(12):1809-1817.
2 Otsuka F ,Yao Z ,Lee T ,Yamamoto S ,Erickson G F ,Shimasaki S. Bone Morphogenetic Protein-15 , Identification of target cells and biological functions[J]. The Journal of Biological Chemistry , 2000 , 275 (50): 39523-39528.
3 Yan C ,Wang P ,DeMayo J ,DeMayo F J ,Elvin J A ,Carino C ,Prasad SV ,Skinner S S ,Dunbar B S ,Dube J L ,Celeste A J ,Matzuk M M. Synergistic role of Bone Morphogenetic Protein-15 and Growth Differentiation Factor 9 inovarian function[J].Molecular Endocrinology , 2001 ,15(6) :854-866.
4 Galloway S M ,McNatty K P ,Cambridge L M ,Laitinen M P ,Juengel JL ,Jokiranta T S ,McLaren RJ ,Luiro K,Dodds K G,Montgomery GW ,Beattie A E ,Davis G H , Ritvos O. Mutations in An Oocyte derived Growth Factor Gene (BMP15) Cause Increased Ovulation Rate and Infertility in A Dosage sensitive Manner [J]. Nature Genetics , 2000, 25(7) :279-282.
5 Montgomery GW ,Galloway S M ,Davis G H ,McNatty K P. Genes Controlling Ovulation Rate in Sheep [J]. Reproduction ,2001 ,121 :843-852.
6 Patricia Ducy ,Gerard Karsenty. The family of bone morphogenetic proteins.Kideny Interational ,2000 ,57 :2207-2214.
7 Otsuka F ,Yamamoto S ,Erickson G F ,Shimasaki S. Bone MorphogeneticProtein-15 Inhibtts Follicle2stimulating Hormone ( FSH) Action by Suppressing FSH Receptor Expression [J]. The Journal of Biological Chemistry ,2001 ,276(14) :11387-11392.
8 Ruggiu M ,Speed R , Taggart Mckay S J , Kilancwski F , Saunders P ,Dorin J ,Cooke H J .The mouse Dazla gene encodes a cytoplasmic protein essential for gameto genesis[J].Nature,1997,389 :73-76.
9 Soyal S M ,Amleh A ,Dean J . FIG(α) ,a germ cellspecific transcription factor required for ovarian follicle formation [J]. Development , 2000 ,127 :4645-4654.
10 Dong J ,Albertini D F ,Nishimori K, Kumar T R ,Lu N ,Matzuk M M.Growth differentiation factor29 is required during early ovarian folliculo genesis[J].Nature,1996, 383 :531-535.
11 Kumar T R,Wang Y,Lu N ,Matzuk M M. Follicle stimulating hormone is required for ovarian follicle maturation but not male fertility[J].Nature Genetics,1997, 15 : 201 -204.
12 Dinchuk J E ,Car B D ,Focht R J ,Johnston J J ,Jaffee B D ,Covington M B ,ContelN R ,Eng V M ,Collins R J ,Czerniak P M ,Gorry S A ,Trzaskos J M. Renal abnormalities and an altered inflammatory response in mice lacking cyclooxygenase II[J]. Nature 1995 ,378 :406-409.
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14 Tong ZB ,Gold L ,Pfeifer K E ,Dorward H ,Lee E ,Bondy C A ,Dean J ,Nelson L M. Mater ,a materal effect gene required for early embryonic development in mice[J]. Nature Genetics , 2006 ,26 :267-268.
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19 Ruberte E, Marty T, Nellen D, Affolter M, Basler K.An absolute requirement for both the type II and type I receptors, punt and thick veins,feo dpp signaling in vivo. Cell.1995 80(6):889-897
20 Kawabata M, Chytil A, Moses HL, Cloning of a novel type II serine/threonine kinase receotor through interaction with the type I transforming growyh factor-beta receptor.J Biol Chem.1995,270(10):5625-5630
21 Wilson T,Wu XY,Juengel JL,Ross IK,Lumsden JM,Lord EA,Dodds KG,Walling GA,Mc Ewan JC,O,Connell AR,McNatty KP,Montgomery GW,Highly prolific Boorola sheep have a mutation in the intracellular kinase domain of bone morphogenetic protein IB receptor (ALK-6) that is expressed in both oocytes and granulose cells.Biol Reprod. 2001, 64(4):125-1235
22 王启贵,钟发刚,李辉,等.羊 BMPR-IB 基因多态性与其产羔数的相关研究[J].草食家畜,2003, 2:20-23.
23 Davis G H, McEwan J C, Fennessy P F, et al. Evidence for the presence of a major gene influencing ovulation rate on the X chromosome of sheep[J].Biol Report, 1991, 44(4):620-624.
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54 Hanrahan P J, Gregan S M, Mulsant P, et al. Mutation in the genes for oocyte-derived growth factors GDF9 and BMP15 are associated with both increased ovarian rate and sterility in Cambridge and Belclare sheep(Ovis aries) [J]. Biol Report, 2004,70(4): 900-909.
55 Mulsant P ,Lecerf F ,Fabre S ,Schibler L ,et al.Mutation in bone morphogenetic protein receptor-IB is associated with increased ovulation rate in Booroola Merino ewes[J]. Proc Natl Acad Sci USA ,2001,98(9):5104-5109.
56 Souza C J ,MacDougall C ,Campbell B K, et al.The Booroola (FecB) phenotype is associated with a mutation in the bone morphogenetic receptor type 1B(BMPR1B)gene[J].J Endocrinol ,2001,169(2):1-6.
57 Wilson T , Wu X Y, Juengel J L , et al. Highly prolific Booroola sheep have a mutation in the intracellular kinase domain of bone morphogenetic protein IB receptor (ALK-6) that is expressed in both oocytes and granulose cells [J]. Biol Reprod ,2001,64 : 1225-1235.
58 Davis G H , Galloway S M , Ross I K, et al. DNA tests in prolific sheep from eight countries provide new evidence on origin of the Booroola ( FecB) mutation [J]. Biol Reprod,2002,66:1869-1874.
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61 王启贵,钟发刚,李 辉,等.羊 BMPR-IB 基因多态性与其产羔数的相关研究[J].草食家畜,2003, 2:20-23.
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2 Otsuka F ,Yao Z ,Lee T ,Yamamoto S ,Erickson G F ,Shimasaki S. Bone Morphogenetic Protein-15 , Identification of target cells and biological functions[J]. The Journal of Biological Chemistry , 2000 , 275 (50): 39523-39528.
3 Yan C ,Wang P ,DeMayo J ,DeMayo F J ,Elvin J A ,Carino C ,Prasad SV ,Skinner S S ,Dunbar B S ,Dube J L ,Celeste A J ,Matzuk M M. Synergistic role of Bone Morphogenetic Protein-15 and Growth Differentiation Factor 9 inovarian function[J].Molecular Endocrinology , 2001 ,15(6) :854-866.
4 Galloway S M ,McNatty K P ,Cambridge L M ,Laitinen M P ,Juengel JL ,Jokiranta T S ,McLaren RJ ,Luiro K,Dodds K G,Montgomery GW ,Beattie A E ,Davis G H , Ritvos O. Mutations in An Oocyte derived Growth Factor Gene (BMP15) Cause Increased Ovulation Rate and Infertility in A Dosage sensitive Manner [J]. Nature Genetics , 2000, 25(7) :279-282.
5 Montgomery GW ,Galloway S M ,Davis G H ,McNatty K P. Genes Controlling Ovulation Rate in Sheep [J]. Reproduction ,2001 ,121 :843-852.
6 Patricia Ducy ,Gerard Karsenty. The family of bone morphogenetic proteins.Kideny Interational ,2000 ,57 :2207-2214.
7 Otsuka F ,Yamamoto S ,Erickson G F ,Shimasaki S. Bone MorphogeneticProtein-15 Inhibtts Follicle2stimulating Hormone ( FSH) Action by Suppressing FSH Receptor Expression [J]. The Journal of Biological Chemistry ,2001 ,276(14) :11387-11392.
8 Ruggiu M ,Speed R , Taggart Mckay S J , Kilancwski F , Saunders P ,Dorin J ,Cooke H J .The mouse Dazla gene encodes a cytoplasmic protein essential for gameto genesis[J].Nature,1997,389 :73-76.
9 Soyal S M ,Amleh A ,Dean J . FIG(α) ,a germ cellspecific transcription factor required for ovarian follicle formation [J]. Development , 2000 ,127 :4645-4654.
10 Dong J ,Albertini D F ,Nishimori K, Kumar T R ,Lu N ,Matzuk M M.Growth differentiation factor29 is required during early ovarian folliculo genesis[J].Nature,1996, 383 :531-535.
11 Kumar T R,Wang Y,Lu N ,Matzuk M M. Follicle stimulating hormone is required for ovarian follicle maturation but not male fertility[J].Nature Genetics,1997, 15 : 201 -204.
12 Dinchuk J E ,Car B D ,Focht R J ,Johnston J J ,Jaffee B D ,Covington M B ,ContelN R ,Eng V M ,Collins R J ,Czerniak P M ,Gorry S A ,Trzaskos J M. Renal abnormalities and an altered inflammatory response in mice lacking cyclooxygenase II[J]. Nature 1995 ,378 :406-409.
13 Lydon J P ,Demayo FJ ,Funk C R ,Mani S K,Hughes A R ,MontgomeryC A ,Shyamala G,Conneely O M ,O ′ Malley B W. Mice lacking progesterone receptor exhibit pleiotropic reproductive abnormalities[J]. Genes Dev ,1995 ,9 :2266- 2278.
14 Tong ZB ,Gold L ,Pfeifer K E ,Dorward H ,Lee E ,Bondy C A ,Dean J ,Nelson L M. Mater ,a materal effect gene required for early embryonic development in mice[J]. Nature Genetics , 2006 ,26 :267-268.
15 Grapes L ,Rothschild M F. BMP15 maps to X chormose in swine[J].Animal Genetics , 2002 ,133(3) :165-166.
16 吴双庆.骨形态发生蛋白-6 的研究进展.国家医学遗传学册.2003,26,(6):350-353
17 李双良.骨形态发生蛋白研究进展.中国实验动物学杂志.1996,6(4):222-227
18 Penton A,Chen Y, Staehling-Hampton K, Wrana JL, Attisano L, Szidonya J, Cassill JA, Massague J,Hoffmann FM. Identification of two bone morphogenetic type I repectors in Drosophila and evidence that Brk25D is a decapentaplegic receptor. Cell.1994, 278(2):239-250
19 Ruberte E, Marty T, Nellen D, Affolter M, Basler K.An absolute requirement for both the type II and type I receptors, punt and thick veins,feo dpp signaling in vivo. Cell.1995 80(6):889-897
20 Kawabata M, Chytil A, Moses HL, Cloning of a novel type II serine/threonine kinase receotor through interaction with the type I transforming growyh factor-beta receptor.J Biol Chem.1995,270(10):5625-5630
21 Wilson T,Wu XY,Juengel JL,Ross IK,Lumsden JM,Lord EA,Dodds KG,Walling GA,Mc Ewan JC,O,Connell AR,McNatty KP,Montgomery GW,Highly prolific Boorola sheep have a mutation in the intracellular kinase domain of bone morphogenetic protein IB receptor (ALK-6) that is expressed in both oocytes and granulose cells.Biol Reprod. 2001, 64(4):125-1235
22 王启贵,钟发刚,李辉,等.羊 BMPR-IB 基因多态性与其产羔数的相关研究[J].草食家畜,2003, 2:20-23.
23 Davis G H, McEwan J C, Fennessy P F, et al. Evidence for the presence of a major gene influencing ovulation rate on the X chromosome of sheep[J].Biol Report, 1991, 44(4):620-624.
24 Mulsant P ,Lecerf F ,Fabre S ,Schibler L ,et al.Mutation in bone morphogeneticprotein receptor-IB is associated with increased ovulation rate in Booroola Merino ewes[J]. Proc Natl Acad Sci USA ,2005,98(9): 5104-5109.
25 Souza C J ,MacDougall C ,Campbell B K, et al.The Booroola (FecB) phenotype is associated with a mutation in the bone morphogenetic receptor type 1B gene[J].J Endocrinol,2001,169(2):1-6.
26 Shimasaki S,Zachow RJ,Li D,Kim H,Iemura S,Ueno N,Sampath K,Chang RJ,Erickson GF.A functional bone morphogenetic protein system in the ovary.Proc Natl Acad Sci USA. 1999,96(13):7282-7287
27 Wilkie PJ,Paszek AA,Beattie CW,Alexander LJ,Wheeler MB,Schook LB.A genomic scan of porcine reproductive traies reveals possible quantitative trait Loci (QTLs) for number of corpora lutea[J] Mammalian Genome,1999,10(6):573-579
28 Montgomery GW, Galloway SM, Davis GH, McNatty KP. Genes controlling ovulation rate in sheep.Reproduction.2003,121(6):843-852
29 Galloway SM,Cambridge LM,Henry HM,van Stijn TC,Davis GH.A genetic test to identify carriers of the ovine Inverdale fecundity gene.Proc N Z Soc Anim Prod 1999,59:114-116
30 Galloway SM, McNatty KP, Cambridge LM,Laitinen MP,Juengel JL,Jokiranta TS,McLaren RJ,Luiro K,Dodds KG,Montgomery GW,Beattie AE,Davis GH,Ritvos O.Mutation ovulation rate and infertility in a dosage-sensitive manner.Nat Genet.2000,25(3):279-283.
31 Brown WM.Mechanism of evolution in animal mitochondrial DNA[J]. AnnN.Y.Acad.Sci, 1981,361:119-134
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