5-LOX和SURVIVIN在胆囊癌中的表达及意义
摘要
目的检测5-LOX和survivin在胆囊癌中表达情况;探讨5-LOX和survivin两种蛋白表达是否具有相关性;结合临床资料,了解5-LOX和survivin与临床资料的关系,为胆囊癌的早期诊断和治疗提供理论依据。
方法标本来自我院肝胆外科自2000年6月至2009年6月行手术切除治疗且病理诊断明确的32例胆囊癌患者的组织标本,详细记录患者的临床资料。采用免疫组化链霉菌抗生物素蛋白-过氧化物酶(Streptavidin-peroxidase,S-P)法检测32例胆囊癌及相应的癌旁组织和20例正常对照慢性胆囊炎组织中5-LOX和survivin蛋白的表达状况,结合临床资料进行统计学分析。
结果癌组织中5-LOX和survivin高表达均明显高于相应的癌旁组(P <0.05;P <0.05)和正常对照组,癌旁组与正常对照组相比,差异也具有显著性(P =0.001;P =0.001)。癌组织和癌旁组织中5-LOX和survivin的表达均存在正相关(P <0.05;P <0.05)。
结论5-LOX和survivin与胆囊癌的形成关系密切。5-LOX和survivin与胆囊癌形成可能存在协同作用。
Objective Detemining the expression of 5-LOX and survivin. Analyze the expression of 5-LOX and survivin in gallbladder carcinoma and their relationship with clinical date. To elucidate the mechanisms for early diagnosis and clinical application.
Methods The specimens were taken from32 patients with gallbladder carcinoma in An Hui Medical Afflicated Hospital from june 2006 to june 2009 Record the clicical date in detail. The expression of 5-LOX and survivin was assayed by Streptavidin-peroxidase immunohistochemistry in 32 gallbladder carcinomas and adjacent non-cancerous tissues,20 normal gallbladder tissue as control. Experimental date were analyzed together with the clinical pathological datum.
Results The expression of 5-LOX and survivin in gallbladder carcinomas was significantly than adjacent non-cancerous tissues(P <0.05;P <0.05) and nomal control tissues,and there was significant difference between adjacent non-cancerous tissues and nomal control tissues(P =0.001;P =0.001).positive correlation were found between the expression of 5-LOX and survivin in gallbladder carcinomas and adjacent non-cancerous tissues(P <0.05;P <0.05).
Conclusion 5-LOX and survivin play a important role in the formation of gallbladder carcinoma. There may be some extend coordinated function in the formation of gallbladder carcinoma.
方法标本来自我院肝胆外科自2000年6月至2009年6月行手术切除治疗且病理诊断明确的32例胆囊癌患者的组织标本,详细记录患者的临床资料。采用免疫组化链霉菌抗生物素蛋白-过氧化物酶(Streptavidin-peroxidase,S-P)法检测32例胆囊癌及相应的癌旁组织和20例正常对照慢性胆囊炎组织中5-LOX和survivin蛋白的表达状况,结合临床资料进行统计学分析。
结果癌组织中5-LOX和survivin高表达均明显高于相应的癌旁组(P <0.05;P <0.05)和正常对照组,癌旁组与正常对照组相比,差异也具有显著性(P =0.001;P =0.001)。癌组织和癌旁组织中5-LOX和survivin的表达均存在正相关(P <0.05;P <0.05)。
结论5-LOX和survivin与胆囊癌的形成关系密切。5-LOX和survivin与胆囊癌形成可能存在协同作用。
Objective Detemining the expression of 5-LOX and survivin. Analyze the expression of 5-LOX and survivin in gallbladder carcinoma and their relationship with clinical date. To elucidate the mechanisms for early diagnosis and clinical application.
Methods The specimens were taken from32 patients with gallbladder carcinoma in An Hui Medical Afflicated Hospital from june 2006 to june 2009 Record the clicical date in detail. The expression of 5-LOX and survivin was assayed by Streptavidin-peroxidase immunohistochemistry in 32 gallbladder carcinomas and adjacent non-cancerous tissues,20 normal gallbladder tissue as control. Experimental date were analyzed together with the clinical pathological datum.
Results The expression of 5-LOX and survivin in gallbladder carcinomas was significantly than adjacent non-cancerous tissues(P <0.05;P <0.05) and nomal control tissues,and there was significant difference between adjacent non-cancerous tissues and nomal control tissues(P =0.001;P =0.001).positive correlation were found between the expression of 5-LOX and survivin in gallbladder carcinomas and adjacent non-cancerous tissues(P <0.05;P <0.05).
Conclusion 5-LOX and survivin play a important role in the formation of gallbladder carcinoma. There may be some extend coordinated function in the formation of gallbladder carcinoma.
引文
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[32] Liotta LA, Steeg PS, Stetler-Stevenson WG. Cancer metastasis and angiogenesis: an imbalance of positive and negative regulation. Cell 1991;64:327-336
[33] Wang Y, Zhou B, Li J, Cao YB, Chen XS, Cheng MH, Yin M. inhibitors of 5-lipoxygenase inhibit expresstion of intercellular adhesion molecule-1 in human melanoma cells. Acta pharmacol Sin 2004;25:672-677
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(2)张林,邹声泉.胆囊腺瘤.腺癌中的DNA含量和基因表达[ J ] ,中华肝胆外科杂志,2004,10 ( 7 ) :460 - 462
(3).陈炯,徐荣楠,余建伟,等.性激紊受体在胆囊癌中的表达研究[ J ].中华普通外科杂志,2001,16( 5) :299—300
(4).石景森.胆结石与胆囊癌.中国实用外科杂志, 1995, 15:12
(5). Gupta S, Snvastava M, Ahmad N, et al. Lipoxygenase-5 is overexpressed in prostate adenocarcinoma. Cancer, 2001;91(4):737-743.
(6). Henning R, Ding XZ, Tong WG, et al. 5-Lipoxygenase and leukotriene B(4) receptor are expressed in human pancreatic cancer but not in pancreatic ducts in pormal tissue. Am J Pathol,2002;161(2):421-428.
(7). Hong SH, Avis I, Vos MD, et al. Relationship of arachidonic aicd metabolizing enzyme expression in epuhelial cancer cell lines to the growth effect of selective biochemical inhibitors. Cancer Res, 1999;59(9):2223-8
(8). Henning R, Ding XZ, Tong WG, et al. 5-lipoxygenase and leukotriene B(4) receptor are expressed in human pancreatic cancers but not in pancreatic ducts in normal tissue. Am J Pathol,2002,161(2):421-8.
(9). Avis I, Hong SH, Martinez A, et al. Five-lipoxygenae inhibitors can mediate apoptosis in human breast cell lines through complex eicosanoid interactions. FASEB J, 2001,15(11):2007-9.
(10). Suzuki A, Kawano H.Survivin initiates procaspase 3/P21 complex formation as a result of interaction with CDK4 to resist Fas-mediated cell death. J Oneogene,2000,19(10):1346-1353.
(11). Oconnor DS,Schechner S,Adida C,et al.Control of apoptosis during angiogenesisby survivin expression in endothelial cell.Am J Pathol,2002,277(5):3247-3257.
(12). Radmark OP. The molecular biology and regulation of 5-lipoxygenase [J ] . A m J Resp ir Crit CareM ed , 2000, 161 (2) : S11-S15
(13). Zhou B, Chen XS, Yin M. Putative role of 5-lipoxygenase in central nervous system [J ] . P rog Physiol Sci, 2003, 34 (1) : 77-79
(14).Funk CD.Prostaglandins and leukotrienes:advances in eicosanoid biology.science 2001;294:1871-1875.
(15). Cao Y, Prescott SM. Many actions of cyclooxygenase-2 in cellular dynamics and in cancer. J cell physiol 2002;190:279-286
(16). Fischer L, Poeckel D, Buerkert E,et al. Inhibitors of actin polymerisation stimulate arachidonic acid release and 5-lipoxygenase activation by upregulation of Ca2+ mobilisation in polymorphonuclear leukocytes involving Src family kinases. Biochim Biophys Acta,2005;1736(2):109-119.
(17). Hoque A, Lippman SM, Wu TT, et al. Increased 5-lipoxygenase expression and induction of apoptosis by its inhibitors in esophageal cancer: a potential target for prevention. Carcinogenesis,2005,26(4):785-791.
(18). Myers CE, Ghosh J. Lipoxygenase inhibition in prostate cancer. Eur Urol,1999;35(5-6):395-398.
(19).Romano M, Catalano A, Nutini M. 5-lipoxygenase regulates malignant mesothelial cell survival:involvement of vascular endothelial growth factor. FASEB J,2001;15(13):2326-2336.
(20). Hong SH, Avis I, Vos MD, et al. Relationship of arachidonic aicd metabolizing enzyme expression in epuhelial cancer cell lines to the growth effect of selective biochemical inhibitors. Cancer Res, 1999;59(9):2223-2228.
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