猪链球菌分子分型新方法研究及健康猪群猪链球菌菌株耐药性检测
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摘要
根据国外当前的多位点序列分型(multilocus sequence typing,MLST)法,我国分离的猪链球菌S. suis 98HAH33和05ZYH33株属于序列7(sequence type 7,ST7)型。但S. suis 98HAH33和05ZYH33在7个猪链球菌MLST基因序列上的基因分子特征却与MLST ST7型其它猪链球菌分离株的基因分子特征不相吻合。
鉴于上述原因,本研究尝试建立一种新的分型方法,力求同一型内的菌株在ST和基因分子特征方面获得一致。本研究首先从基因组上随机选择6个保守的酶基因和1个CPS基因,截取其500-750bp的保守片段。由于相关研究认为GenBank上发表的S. suis 98HAH33和S. suis 05ZYH33基因组序列存在较多的错误,所以,我们首先对本课题保存的S. suis 98HAH33和S. suis 05ZYH33菌株上的相关基因测序,以求得到准确的测序结果。同时,从GenBank上下载S. suis BM407,S. suis P1/7,S. suis SC84相关序列。之后,按C76和C77组合,将其按6或7片段连接起来,通过DNASTAR软件MegAlign建立遗传树。该分型法对所得的8个分型图均表明,S. suis 98HAH33、S. suis 05ZYH33均应归属于MLST ST1型,分型结果与S. suis 98HAH33、S. suis05ZYH33的基因分子特征完全吻合。
同时,本研究从甘肃省部分地区采集到的152份健康猪扁桃体分离猪链球菌,共分离到67株猪链球菌菌株,分离率约44%。对67株猪链球菌菌株所作的耐药性检测表明,全部猪链球菌分离株具有多重耐药性,最严重者,对22种抗生素中的16种抗生素产生耐药性。用我国致病性2型猪链球菌特有的89k毒力岛序列特异性引物对67株猪链球菌进行PCR鉴定,仅发现一株猪链球菌分离株能够扩增出该特异性片段,提示在该地区健康猪群中少数猪可能隐性感染致病性2型猪链球菌病。
In previous research, two invasive Chinese strains, S. suis 98HAH33 and S. suis 05ZYH33 were clustered into MLST ST7. However, the molecular characteristic of these two strains is inconsistent with other strains in MLST ST7. In this research, six housekeeping gene and CPS fragments of five invasive S. suis strains was obtained and the fragments were concatenated together as C76and C77. Then the concatenated sequences were used for phylogenetic tree analysis using DNA star software to resubtyping these popular S.suis isolates. Eight phylogenetic trees were obtained in this study and analysis of these trees revealed that the invasive S. suis strains isolated in China all belong to MLST ST1, and they all have a close relationship with S. suis 1/7. At the same time,they differed obviously from S. suis BM407 ,which was also belongd to MLST ST1. this result is in agree with the the molecular characteristic and genome analysis of these strains.
In this research, 67 S. suis isolates was isolated from 152 of the tonsils from healthy slaughting pigs that was come from Wuwei, Lanzhou and Qingyang of Gansu province,PRC. Antimicrobial susceptibility test using disk diffusion showed that all of the bacterials isolated were multiple-antibiotic resistant, and for one isolates,it resistance to 16 of the 22 antibiotics detected. PCR was performed to investigat whether these isolates have a 89k pathogenicity island (PAI) 89K which was specific for Chinese SS2 virulent isolates or not.
鉴于上述原因,本研究尝试建立一种新的分型方法,力求同一型内的菌株在ST和基因分子特征方面获得一致。本研究首先从基因组上随机选择6个保守的酶基因和1个CPS基因,截取其500-750bp的保守片段。由于相关研究认为GenBank上发表的S. suis 98HAH33和S. suis 05ZYH33基因组序列存在较多的错误,所以,我们首先对本课题保存的S. suis 98HAH33和S. suis 05ZYH33菌株上的相关基因测序,以求得到准确的测序结果。同时,从GenBank上下载S. suis BM407,S. suis P1/7,S. suis SC84相关序列。之后,按C76和C77组合,将其按6或7片段连接起来,通过DNASTAR软件MegAlign建立遗传树。该分型法对所得的8个分型图均表明,S. suis 98HAH33、S. suis 05ZYH33均应归属于MLST ST1型,分型结果与S. suis 98HAH33、S. suis05ZYH33的基因分子特征完全吻合。
同时,本研究从甘肃省部分地区采集到的152份健康猪扁桃体分离猪链球菌,共分离到67株猪链球菌菌株,分离率约44%。对67株猪链球菌菌株所作的耐药性检测表明,全部猪链球菌分离株具有多重耐药性,最严重者,对22种抗生素中的16种抗生素产生耐药性。用我国致病性2型猪链球菌特有的89k毒力岛序列特异性引物对67株猪链球菌进行PCR鉴定,仅发现一株猪链球菌分离株能够扩增出该特异性片段,提示在该地区健康猪群中少数猪可能隐性感染致病性2型猪链球菌病。
In previous research, two invasive Chinese strains, S. suis 98HAH33 and S. suis 05ZYH33 were clustered into MLST ST7. However, the molecular characteristic of these two strains is inconsistent with other strains in MLST ST7. In this research, six housekeeping gene and CPS fragments of five invasive S. suis strains was obtained and the fragments were concatenated together as C76and C77. Then the concatenated sequences were used for phylogenetic tree analysis using DNA star software to resubtyping these popular S.suis isolates. Eight phylogenetic trees were obtained in this study and analysis of these trees revealed that the invasive S. suis strains isolated in China all belong to MLST ST1, and they all have a close relationship with S. suis 1/7. At the same time,they differed obviously from S. suis BM407 ,which was also belongd to MLST ST1. this result is in agree with the the molecular characteristic and genome analysis of these strains.
In this research, 67 S. suis isolates was isolated from 152 of the tonsils from healthy slaughting pigs that was come from Wuwei, Lanzhou and Qingyang of Gansu province,PRC. Antimicrobial susceptibility test using disk diffusion showed that all of the bacterials isolated were multiple-antibiotic resistant, and for one isolates,it resistance to 16 of the 22 antibiotics detected. PCR was performed to investigat whether these isolates have a 89k pathogenicity island (PAI) 89K which was specific for Chinese SS2 virulent isolates or not.
引文
[1]蔡宝祥.家畜传染病学[M].2版.北京:中国农业出版社,1991:141-4.
[2] Touil F,Higgin R,Nadeau M.A Isolation of Streptococcus suis from diseased pigs in Canada[J].Vet Micro- biol,1988,17:171-7.
[3]蔡宝祥.家畜传染病学[M].2版.北京:农业出版社,1991:186-90.
[4]黄静,张苏强.猪溶血性链球菌诊疗报告[J].中国兽医杂志,1992,18(1):22-3.
[5] Wang R, Braughton KR, Kretschmer D,et,al. Identification of novel cytolytic peptides as key virulence de- terminants for community-associated MRSA[J]..Nat Med. 2007 Dec;13(12):1510-4.
[6]闻清波,苑青艳,赵志惠,等.常用抗菌药物对东北地区猪源链球菌的体外抗菌活性研究,中国预防兽医学报,2007,29(12):977-80.
[7]常洪涛,姚雪静,陈陆,等,河南省致病性猪源链球菌部分微生物学特性及耐药性研究安徽农业科学, 2008 ,36 (22) :9533-5,9545.
[8]李一经.人兽共患2型猪链球菌病研究概况[J].黑龙江畜牧兽医.2005,(9):5-6.
[9] Chen C, Tang J, Dong W, et al. (2007) A Glimpse of Streptococcal Toxic Shock Syndrome from compare- ative Genomics of S. suis 2 Chinese Isolates. PLoS ONE 2(3): e315.
[10] Davies PR, Ossowicz CJ. Evaluation of methods used for detecting Streptococcus suis type 2 in tonsils, and investigation of the carrier state in pigs[J]. Res Vet Sci. 1991 Mar;50(2):190-4.
[11] Sandy amass ,Marcelo Gottschalk ,Lisa Tokach ,et al. Streptococcus suis a common cohabitant[J] . Pig progress ,2001 ,9 :8-9.
[12] Janet E. Hill , Marcelo Gottschalk , Roland Brousseau , et al. Swee Han Goh Biochemical analysis, cpn60 and 16S rDNA sequence data indicate that Streptococcus suis serotypes 32 and 34, isolated from pigs, are Streptococcus orisratti [J].Veterinary Microbiology 107 (2005) 63-9.
[13]李军,牛钟相. 2004.猪链球菌病研究进展.动物医学进展[J]. 25(3):31-3.
[14]沈健,孙建中,尤玉民等. 2002.如皋市人~猪链球菌感染综合征预防控制效果分析.数理医药学杂志[J]. 13(3):257-8.
[15]郑世军,杨汉春.猪病免疫与防治技术[M].北京:北京农业大学出版社,1995.
[16] Staats J,FederI,Okwumabua O,et al.Streptococcuus suis Past and Preasent[J].Vet Res Commun ,1997, 21(6):381-407.
[17] Inmaculada Luque,Verena Blume,Carmen Borge , et al. Genetic analysis of Streptococcus suis isolates recovered from diseased and healthy carrier pigs at different stages of production on a pig farm[J] .The Veterinary Journal.
[18] Galina L,Vecht U, Wisselink HJ,et al. Prevalence of various phenotypes of Streptococcus suis isolated from swine in the U.S.A. based on the presence ofmuraminidase-released protein and extracellular factor [J]. Can J Vet Res . 1996,60:72-4.
[19]王继春,何孔旺,何家惠等. 2001. 2型猪链球菌动物致病性试验[J].中国兽医科技.31(12):23-5.
[20] Gottschalk M , Segura M. The pathogenesis of the meningitis caused by Streptococcus suis: the unresol-ved questions[J]. Veterinary Microbiology. 2000,76, 259-72.
[21] Kataoka Y, Yoshida T, Sawada T. A 10-year survey of antimicrobial susceptibility of Streptococcus suis isolates from swine in Japan. J[J]. Vet. Med. Sci. 2000,62, 1053-7.
[22] Smith HE,de Vries R,van’t Slot R.The cps locus of Streptococcus suis serotype2:genetic determinant for the synthesis of sialic acid[J]Microb Pathog,2000,29:127-34.
[23] Segers RP,Kenter T,deHaan LA.Characterisation of the gene encoding suilysin from Streptococcus suis and expressionin field strains[J].FEMS Microbiol Lett,1998,167:255-61.
[24] Smith HE,Veenbergen V,van der Velde J.The cps genes of Streptococcus suis serotype1,2 and 9:develop- ment of rapid serotype-specific PCR assays[J].JClin Microbiol,1999,37:3146-52.
[25] Charland N,Kobisch M,Martineau-Doize B.Role of capsular sialic acid in virulence and resistance to pha- gocytosis of Streptococcus suis capsular type2[J].FEMS Immunol Med Microbiol,1996,14:195-203.
[26] Charland N,Jacques M,Lacouture S.Characterization and protective activity of a monoclonal antibody ag- ainst a capsular epitope shared by Streptococcus suis serotype1,2 and 1/2[J]. Microbiology, 1997,143: 3607-14.
[27] Del Campo Sepulveda EM. Detection of antibodies against Streptococcus suis serotype2 using a purified capsular polysaccharide antigen-based indirect ELISA[J].Vet Microbiol, 1996,52: 113-25.
[28] Vecht U,Stockhofe-Zurwieden N,Teteburg BJ,et,al.Virulence of Streptococcus suis type2 for mice and pigs appeared host-specific[J].Vet Microbiol,1997,58:53-60.
[29]曾巧英,陆承平.用单克隆抗体展示猪链球菌2型溶菌酶释放蛋白在菌体表面的形态和分布[J].中国农业科学,2004,37(1):143-7.
[30]曾巧英,陆承平.猪链球菌2型溶菌酶释放蛋白的粘附作用[J].南京农业大学学报,2002,25(4):67-71.
[31]曾巧英,陆承平.猪链球菌2型溶菌酶释放蛋白诱导上皮细胞融合和凋亡[J].微生报,2003,43(3):407-12.
[32] Kilpper-Balz R,Schleifer K.Streptococcus suis sp,nov,nom,rev[J].Int J Syst Bacteriol, 1987,37:160-2.
[33] Galina L,Vecht U,Wisselink HJ.Prevalence of various sphenotypes of Streptococcus suis isolated from swine in the USA based on the presence of muraminidase-releases protein and extracellular factor[J].Can J Vet Res,1996,60:72-4.
[34] Luque I,Tarradas C,Astorga R.The presence of muramidase released protein and extracellular factor pro- tein in various serotypes of Streptococcus suis isolated from diseased and healthy pigs in Spain[J].Res Vet Sci,1999,66:69-72.
[35] Gottschal kM,Lebrum A,Wisselink H.Production of virulence-related proteins by Canadian strains of Str- eptococcus suis capsular type2[J].Can J Vet Res,1998, 62:75-9.
[36] Okwumabua O,Abdelmagid O,Chengappa MM.Hybridization analysis of the gene encoding a hemolysin of Sreptococcus suis type2:evidence for the absence of the gene in some isolates[J].FEMS Microbiol Lett, 1999,181:113-21.
[37] Jacobs AA,Loefen PL,Van Den Berg AJ,et al. Identification,purification,and characterization of a thiol-a- ctivated hemolysin(suilysin) of Streptococcus suis[J].Infect Immun,1994,62(5):1742-8.
[38]方绍庆,陆承平.猪链球菌2型溶血素的化学修饰[J].微生物学报,2003,43(3):395-9.
[39] Tenenbaum T,Adam R,Eggelnpohler I,et,al.Strain-dependent disruption of blood-cerebrospinal fluid bar- rier by Sreptococcus suis in vitro[J]. FEMS Immunol Med Microbiol,2005,44(1):25-34.
[40] Vanier G,Segura M,Friedl P,et al.Invasion of porcine brain microvascular endothelial cells by Streptococ- cus suis serotype2[J].Infect Immun, 2004,72(3):1441-9.
[41] Jacobs AAC,van den Berg AJG,Baars JC.Protection of experimentally infected pigs by suilysin,thethiol- activated haemolysin of Streptococcus suis[J].Vet Rec, 1996,139:225-8.
[42] Norton PM,Rolph C,Ward PN.Epithelial invasion and cell lysis by virulent strains of Streptococcus suis is enhanced by the presence of suilysin[J].FEMS Immunol Med Microbiol,1999,26:25-35.
[43] Charland N,Nizet V,Rubens CE.Streptococcus suis serotype2 interactions with human brain microvascula rendothelial cells[J].Infect Immun,2000,68:637-43.
[44] Staats JJ,Plattner BL,Stewart GC.Presence of the Streptococcus suis suilysin gene and expression of MRP and EFcorrelates with high virulence in Streptococcus suis type2 isolates[J].Vet Microbiol,1999,70:201- 11.
[45] Flen AG,Bolitho S,Lindsay H.Generation and characterization of amutant of Streptococcussuis[J].Infect Immun,2001,69:2732-5.
[46] Quessy S,Busque P,Higgins R.Descroption of an albumin binding activity for Streptococcus suis seroty- pe 2[J].FEMS Microbiol Lett,1997,47:245-50.
[47] Haataja S,Tikkanen K,Hytonen J,et al.The Gal alpha 1-4Gal-binding adhesion of Streptococcus suis,a gram-positive meningitis-associated bacterium[J].Adv Exp Med Biol,1996,408:25-34.
[48] Smith HE,Buijs H,Wisselink HJ,et al.Selection of virulence-associated determinants of Streptococcus suis type2 by in vivo complementation[J].Infect Immun,2001,69(3):1961-6.
[49] Astridde G,Herma B,Robin V.Contribution of Fibronectin-Binding Protein to pathogenesis of Streptoco- ccus suis serotype2[J].Infect Immun,2002,70(3): 1319-25.
[50] Michael CF,Jose PC,Philip JW.Investigation of a Novel DNase of Streptococcus suis serotype2[J].Infect Immun,2004,72(2):774-81.
[51]田云,FrankM,陆承平.猪链球菌2型可能的毒力基因的发现[J].微生物学报,2004,44(5):613-6.
[52] Tang J, Wang C, Feng Y, et al. (2006) Streptococcal toxic shock syndrome caused by Streptococcus sui- s serotype 2. PLoS Med 3(e151):0668–76.
[53] Lindsay JA, Ruzin A, Ross HF, Kurepina N, Novick RP (1998) The gene for toxic shock toxin is carried by a family of mobile pathogenicity islands in Staphylococcus aureus. Mol Microbiol 29: 527–43.
[54] Chesney PJ (1983) Toxic-shock syndrome: a commentary and review of the characteristics of Staphyloc- occus aureus strains. Infection 11: 181–8.
[55] Stevens DL, Tanner MH, Winship J, Swarts R, Ries KM, et al. (1989) Severe group A streptococcal inf- ections associated with a toxic shock-like syndrome and scarlet fever toxin A. N Engl J Med 321: 1–7.
[56] Hacker J, Blum-Oehler G, Hochhut B, Dobrindt U (2003) The molecular basis of infectious diseases: pathogenicity islands and other mobile genetic elements. A review. Acta Microbiol Immunol Hung 50: 321–30.
[57] Ubeda C, Tormo MA, Cucarella C, Trotonda P, Foster TJ, et al. (2003) Sip, an integrase protein with ex- cision, circularization and integration activities, defines a new family of mobile Staphylococcus aureus pathogenicity islands. Mol Microbiol 49: 193–210.
[58] Fitzgerald JR, Monday SR, Foster TJ, Bohach GA, Hartigan PJ, et al. (2001) Characterization of a putati- ve pathogenicity island from bovine Staphylococcus aureus encoding multiple superantigens. J Bacteriol 183: 63–70.
[59] Cabello F (1997) Pathogenicity islands: important but not unique factors contributing to Salmonella viru- lence. Trends Microbiol 5: 431–2.
[60] Ochman H, Groisman EA (1996) Distribution of pathogenicity islands in Salmonella spp. Infect Immun 64: 5410–2.
[61] Brown JS, Gilliland SM, Holden DW (2001) A Streptococcus pneumoniae pathogenicity island encoding an ABC transporter involved in iron uptake and virulence. Mol Microbiol 40: 572–85.
[62] Matthew T. G. Holden, Heidi Hauser, Mandy Sanders,Thi Hoa Ngo, et al (2009) Rapid Evolution of Virulence and Drug Resistance in the Emerging Zoonotic Pathogen Streptococcus suis. PLoS ONE 4: e6072.
[63] Lévesque C, PichéL, Larose C, Roy PH. PCR mapping of integrons reveals several novel combinations of resistance genes[J]. Antimicrob Agents Chemother. 1995 Jan;39(1):185-91.
[64] Piddock LJ, Ricci V, McLaren I, et al. Role of mutation in the gyrA and parC genes of nalidixic-acid-re- sistant salmonella serotypes isolated from animals in the United Kingdom. J Antimicrob Chemother. 1998 Jun;41(6):635-41.
[65] Mason EJ,Wald ER,Bradley JS,et al.Macrolide resistance among middle ear isolates of Streptococcus p- neumoniae observed at eight United States pediatric centers:prevalence of M and MLSB phenotypes[J]. Pediatr Infect Dis J,2003,22:623-7.
[66] Bartelink AKM,E van Kregten.Streptococcus suis as threat to pig farmers and abattoir workers[J]. The lancet,1995,346(8991-8992):1707.
[67]蔡宝祥.家畜传染病学(第3版)[M].北京:中国农业出社,1996:115-8.
[68]陆承平,姚火春.科学进步与科学进展(下册)[M].南京:东南大学出版社,1999:339-43.
[69]姚火春,陈国强,陆承平.猪链球菌1998分离株病原特性鉴定[J].南京农业大学学报,1999,22(2):67-70.
[70] Perch B,Kjems E,Slot P,et al.Biochemical and serological properties of R,S and RS streptococci[J].Act a Pathol Microbiol Scand[B],1981,89(3):167-71.
[71] Hoffman LJ,Henderson LM.The signification of streptococcus suis in swine diseases:clinical,pathologic and bacteriologic date from a two year study[J].Proc Assoc Vet Lab Diagn,1985:201-10.
[72] Hommez J,Devriese LA,Henrichsen J,et al.Identification and characterization of streptococcus suis[J]. Vet Microbiol,1986,11(4):349-55.
[73] Sihvonen L,Kurl D N,Henrichen J. Streptococcus suis isolated from pigs in Finland[J].Act a Vet Scand, 1988,29(1):9-13.
[74] Higgins R,Gottschalk M,Mittal K R,et al. Streptococcus suis infecton in swine.A sixteen-month study[J]. Can J Vet Res,1990:54(1):170-3.
[75] Devriese LA,Ceyssens K,Hommez J,et al.Characteristics of different Streptococcus suis ecovars and des- cription of a simplified identification method[J].Vet Microbiol, 1991,26 (1-2):141-50.
[76] Tarradas C,Arenas A,Maldonado A,et al. Identification of Streptococcus suis isolated from swine:propos- al for biochemical parameters[J].J Clin Microbiol, 1994,32 (2):578-80.
[77] Oliver Goldmann,Gursharan S Chhatwal,Eva Medina.Contribution of Natural Killer Cells to the Pathoge- nesis of Septic Shock Induced by Streptococcus pyogenes in Mice[J].The Journal of Infectious Diseases Chicago,2005,191:1280-6.
[78] Manuel J Rodrguez Ortega,Nathalie Norais,Giuliano Bensi,et al.Characterization and identification of va- ccine candidate proteins through analysis of the group A Streptococcus surface proteome[J].Nature Biote- chnology New York,2006,24:191-7.
[79] Martinez G,Harel J,Higgins R,et al.Characterization of Streptococcus ag alactiae isolates of bovine andhuman origin by randomly amplified polymorphic DNA analysis[J]. Microbiol,2000,38:71-8.
[80] Martinez G,Harel J,Lacouture S, et al.Genetic diversity of Streptococcus suis serotypes 2 and 1/2 isolates recovered from carrier pigs in closed herds[J]. Can J Vet Res. 2002 Oct;66(4):240-8.
[81]李国生,杨晓阳,赵海金.中西医结合扑灭猪爆发性链球菌病[J].中国兽疫学杂志,2001,(1):19.
[82] Mwaniki CG,Robertson I D,Trott D J.etal.Clonal analysis and virulence of Australian isolates of Strepto- coccus suis type[J].Epidemiol Infect,1994, 113:321-34.
[83] Brousseau R,Hill J E, Prefontaine G, et al. Streptococcus suissero-types characterized by analysis of chaperonin 60 gene sequences[M].EnvMicrobiol, 2001.
[84]李明,何孔旺,陆承平.检测两种猪源链球菌抗体间接ELISA方法的筛选与应用[J].中国人兽共患病杂志,2005,(10):53-8.
[85]王海丽,徐公义,王长军等。链球菌2型人源分离株MRP单克隆抗体的制备与鉴定[J].中国兽医科学,2008(5):74-81.
[86] Betsy Foxman,Brenda Gillespie,Shannon D,etal.Incidence and Duration of Group B Streptococcus by se- rotype among Male and Female College Students Living in a Single Dormitory[J].American Journal of Epidemiology Oxford,2006,163:544.
[87]陈兴扶,徐瑞,尹燕博,等.用ELISA检测猪链球菌病血清抗体(初报)[J].中国动物检疫,1999,16(4):1-4.
[88]杨俊兴,金梅林,陈剑锋,等.猪链球菌2型抗体快速检测试纸条的制备及初步应用[R].北京:中国畜牧兽医学会2006学术年会,2006.
[89] Boye M,Feenstra A A,Tegtmeier C,etal.Detection of Streptococcus suis by insituhybridization, indirect immunofluorescence,and peroxidase antiper oxidas eassays informalin fixed,paraffin embedded tissues ections from pigs[J].Vet Diagn Invest,2000,12:224-32.
[90] Madsen L W,Boye M,Jensen H E.Anenzyme based in situ hybridization method for theident ification of Streptococcus suis[J].APMIS,2001,109:665-9.
[91] Roman Prymula,Pascal Peeters,Viktor Chrobok,etal.Pneumococcal capsular polysaccharides conjugated to protein D for prevention of Acute otitis media caused by both Streptococcus pneumoniaeandnon typa- ble Haemophilus influenzae:a randomized double blind efficacy study[J].Clin Otolaryngol,2006,31:435.
[92] Ulrike Haugwitz,Wiesia Bobkiewicz,Shan RuiHan,etal.Poreforming Staphylococcus aureus[alpha] toxin triggers epidermal growth factor receptor dependent proliferation[J].Cellular Microbiology,2006,8: 1591-600.
[93] Balakrishnan Prithiviraj,Harsh P Bais,Ajay KJ ha,etal.Staphylococcus aureus pathogenicity on Arabidop sisthaliana is mediated either by a direct effect of salicylic acid on the pathogen or by SA dependent.NP- R1 independent host responses[J].The Plant Journal,2005,42:417-32.
[94] Okwumabua O,Staats J,Chengappa M M.Detection of genomic heterogeneity in Streptococcus suis iso- lates by DNA restriction fragment length polymorphisms of rRNA genes(ribotyping)[J].J Clin Microbiol, 1995,33(4):968-72.
[95]倪艳秀,何孔旺,王继春,等.链球菌2型的快速检测[J].中国兽医学报,2002,22(5):473-6.
[96]倪宏波,欧阳素贞,王兴龙,等.利用随机扩增多态性DNA指纹技术对猪链球菌进行基因分型[J].中国兽医科技,2002,32(7):9-12.
[97]李国生,杨晓阳,赵海金.中西医结合扑灭猪爆发性链球菌病[J].中国兽疫学杂志,2001,(1):19.
[98]赵冉,孙建和,陆承平.多重PCR鉴定猪链球菌2型毒力菌株[J].上海交通大学学报(农业科学版), 2006,24(6):503-28.
[99]田云,Aarestrup F M,陆承平等.猪链球菌2型可能的毒力基因的发现[J].微生物学报,2004,44(5):613-6.
[100]郑腾.2型猪链球菌病检测技术的最新进展[J].福建畜牧兽医,2006,(增刊):23-6.
[101]郑峰,王长军,海攀等.猪链球菌基因芯片检测方法的研究[J].中国人兽共患病学报,2008,24(1):38-41.
[102] Gottschalk M,Segura M.The pathogenesis of the meningit is caused by Streptococcus suis: Unresolved questions[J].Vet Microbiol,2000,76:259-272.
[103] Smith HE,Rijnsburger M,Stockhofe-Zurwieden N, et al.Virulent strains of Streptococcus suis serotype 2 and highly virulent strains of Streptococcus suis serotype 1 can be recognized by a unique ribotype pro- file[J]. J Clin Microbiol. 1997 May;35(5):1049-53.
[104] Corinne Marois,La?titia Le Devendec,Marcelo Gottschalk, et al.Molecular characterization of Streptooc- ccus suis strains by 16S–23S intergenic spacer polymerase chain reaction and restriction fragment length polymorphism analysis[J].The Canadian Journal of Veterinary Research 2006;70:94–104.
[105]Achim Allgaier,Ralph Goethe,Henk J, et al.Relatedness of Streptococcus suis isolates of various serotyp- es and clinical backgrounds as evaluated by macrorestriction analysis and expression of potential virule- nce traits[J]. Journal of Clinical Microbiology Feb. 2001,p. 445–53.
[106] Florence B H,Marois C,Gott schalk M,et al.Geneti diversity of Streptococcus suis strains isolated from pigs and humans as revealed by pulsed-field gel electrophoresis[J].Clin Microbiol,2002,40:615-9.
[107] Wang S M,Smith H E,Rijnsburger M ,et al.Virulent strains of Streptococcus suis serotype 2 and highly virulent strains of Streptococcus suis serotype 1 can be recognized by a unique ribotype profile[J]. Clin Microbiol,2004,35:1049-53.
[108] Ana I V,Joaquin G,Carmen T, et al.Molecular typing by pulsed-field gel electrophoresis of Spanish ani- mal and human Listeria monocytogenes isolates [J].Appl Environ Microbiol,2001,67:5840-3.
[109] Cindy K.Y.Luey,Yiu Wai Chu,Terence K.M.Cheung, et al.Rapid pulsed-field gel electrophoresis proto- col for subtyping of Streptococcus suis serotype 2[J]. Journal of Microbiological Methods 68 (2007) 648– 50.
[110] Inmaculada Luque,Verena Blume,Carmen Borge, et al.Genetic analysis of Streptococcus suis isolates recovered from diseased and healthy carrier pigs at different stages of production on a pig farm [J].The Veterinary Journal.
[111] Enright MC, Spratt BG.Multilocus sequence typing[J]. Trends Microbiol 1999, 7:482-7.
[112] Samantha J. King, James A. Leigh, Peter J. Heath, et al. Development of a Multilocus Sequence Typ- ing Scheme for the Pig Pathogen Streptococcus suis: Identification of Virulent Clones and Potential Ca- psular Serotype exchange[J]. Journal of Clinical Microbiology,Oct.2002,p.3671-80.
[113] Chang B,Wada A,Ikebe T, et al.Characteristics of Streptococcus suis isolated from patients in Japan[J]. Jpn J Infect Dis. 2006 Dec;59(6):397-9.
[114] Rehm T,Baums CG,Strommenger B,Beyerbach M,Valentin-Weigand P,Goethe R.Amplified fragment length polymorphism of Streptococcus suis strains correlates with their profile of virulence-associated genes and clinical background[J].J Med Microbiol. 2007 Jan;56(Pt 1):102-9.
[115] Blume V, Luque I, Vela AI, et al. Genetic and virulence-phenotype characterization of serotypes 2 and 9 of Streptococcus suis swine isolates[J]. Int Microbiol. 2009 Sep;12(3):161-6.
[116] Kerdsin A, Oishi K, Sripakdee S, et al. Clonal dissemination of human isolates of Streptococcus suis serotype 14 in Thailand[J]. J Med Microbiol. 2009 Nov;58(Pt 11):1508-13. Epub 2009 Aug 6.
[2] Touil F,Higgin R,Nadeau M.A Isolation of Streptococcus suis from diseased pigs in Canada[J].Vet Micro- biol,1988,17:171-7.
[3]蔡宝祥.家畜传染病学[M].2版.北京:农业出版社,1991:186-90.
[4]黄静,张苏强.猪溶血性链球菌诊疗报告[J].中国兽医杂志,1992,18(1):22-3.
[5] Wang R, Braughton KR, Kretschmer D,et,al. Identification of novel cytolytic peptides as key virulence de- terminants for community-associated MRSA[J]..Nat Med. 2007 Dec;13(12):1510-4.
[6]闻清波,苑青艳,赵志惠,等.常用抗菌药物对东北地区猪源链球菌的体外抗菌活性研究,中国预防兽医学报,2007,29(12):977-80.
[7]常洪涛,姚雪静,陈陆,等,河南省致病性猪源链球菌部分微生物学特性及耐药性研究安徽农业科学, 2008 ,36 (22) :9533-5,9545.
[8]李一经.人兽共患2型猪链球菌病研究概况[J].黑龙江畜牧兽医.2005,(9):5-6.
[9] Chen C, Tang J, Dong W, et al. (2007) A Glimpse of Streptococcal Toxic Shock Syndrome from compare- ative Genomics of S. suis 2 Chinese Isolates. PLoS ONE 2(3): e315.
[10] Davies PR, Ossowicz CJ. Evaluation of methods used for detecting Streptococcus suis type 2 in tonsils, and investigation of the carrier state in pigs[J]. Res Vet Sci. 1991 Mar;50(2):190-4.
[11] Sandy amass ,Marcelo Gottschalk ,Lisa Tokach ,et al. Streptococcus suis a common cohabitant[J] . Pig progress ,2001 ,9 :8-9.
[12] Janet E. Hill , Marcelo Gottschalk , Roland Brousseau , et al. Swee Han Goh Biochemical analysis, cpn60 and 16S rDNA sequence data indicate that Streptococcus suis serotypes 32 and 34, isolated from pigs, are Streptococcus orisratti [J].Veterinary Microbiology 107 (2005) 63-9.
[13]李军,牛钟相. 2004.猪链球菌病研究进展.动物医学进展[J]. 25(3):31-3.
[14]沈健,孙建中,尤玉民等. 2002.如皋市人~猪链球菌感染综合征预防控制效果分析.数理医药学杂志[J]. 13(3):257-8.
[15]郑世军,杨汉春.猪病免疫与防治技术[M].北京:北京农业大学出版社,1995.
[16] Staats J,FederI,Okwumabua O,et al.Streptococcuus suis Past and Preasent[J].Vet Res Commun ,1997, 21(6):381-407.
[17] Inmaculada Luque,Verena Blume,Carmen Borge , et al. Genetic analysis of Streptococcus suis isolates recovered from diseased and healthy carrier pigs at different stages of production on a pig farm[J] .The Veterinary Journal.
[18] Galina L,Vecht U, Wisselink HJ,et al. Prevalence of various phenotypes of Streptococcus suis isolated from swine in the U.S.A. based on the presence ofmuraminidase-released protein and extracellular factor [J]. Can J Vet Res . 1996,60:72-4.
[19]王继春,何孔旺,何家惠等. 2001. 2型猪链球菌动物致病性试验[J].中国兽医科技.31(12):23-5.
[20] Gottschalk M , Segura M. The pathogenesis of the meningitis caused by Streptococcus suis: the unresol-ved questions[J]. Veterinary Microbiology. 2000,76, 259-72.
[21] Kataoka Y, Yoshida T, Sawada T. A 10-year survey of antimicrobial susceptibility of Streptococcus suis isolates from swine in Japan. J[J]. Vet. Med. Sci. 2000,62, 1053-7.
[22] Smith HE,de Vries R,van’t Slot R.The cps locus of Streptococcus suis serotype2:genetic determinant for the synthesis of sialic acid[J]Microb Pathog,2000,29:127-34.
[23] Segers RP,Kenter T,deHaan LA.Characterisation of the gene encoding suilysin from Streptococcus suis and expressionin field strains[J].FEMS Microbiol Lett,1998,167:255-61.
[24] Smith HE,Veenbergen V,van der Velde J.The cps genes of Streptococcus suis serotype1,2 and 9:develop- ment of rapid serotype-specific PCR assays[J].JClin Microbiol,1999,37:3146-52.
[25] Charland N,Kobisch M,Martineau-Doize B.Role of capsular sialic acid in virulence and resistance to pha- gocytosis of Streptococcus suis capsular type2[J].FEMS Immunol Med Microbiol,1996,14:195-203.
[26] Charland N,Jacques M,Lacouture S.Characterization and protective activity of a monoclonal antibody ag- ainst a capsular epitope shared by Streptococcus suis serotype1,2 and 1/2[J]. Microbiology, 1997,143: 3607-14.
[27] Del Campo Sepulveda EM. Detection of antibodies against Streptococcus suis serotype2 using a purified capsular polysaccharide antigen-based indirect ELISA[J].Vet Microbiol, 1996,52: 113-25.
[28] Vecht U,Stockhofe-Zurwieden N,Teteburg BJ,et,al.Virulence of Streptococcus suis type2 for mice and pigs appeared host-specific[J].Vet Microbiol,1997,58:53-60.
[29]曾巧英,陆承平.用单克隆抗体展示猪链球菌2型溶菌酶释放蛋白在菌体表面的形态和分布[J].中国农业科学,2004,37(1):143-7.
[30]曾巧英,陆承平.猪链球菌2型溶菌酶释放蛋白的粘附作用[J].南京农业大学学报,2002,25(4):67-71.
[31]曾巧英,陆承平.猪链球菌2型溶菌酶释放蛋白诱导上皮细胞融合和凋亡[J].微生报,2003,43(3):407-12.
[32] Kilpper-Balz R,Schleifer K.Streptococcus suis sp,nov,nom,rev[J].Int J Syst Bacteriol, 1987,37:160-2.
[33] Galina L,Vecht U,Wisselink HJ.Prevalence of various sphenotypes of Streptococcus suis isolated from swine in the USA based on the presence of muraminidase-releases protein and extracellular factor[J].Can J Vet Res,1996,60:72-4.
[34] Luque I,Tarradas C,Astorga R.The presence of muramidase released protein and extracellular factor pro- tein in various serotypes of Streptococcus suis isolated from diseased and healthy pigs in Spain[J].Res Vet Sci,1999,66:69-72.
[35] Gottschal kM,Lebrum A,Wisselink H.Production of virulence-related proteins by Canadian strains of Str- eptococcus suis capsular type2[J].Can J Vet Res,1998, 62:75-9.
[36] Okwumabua O,Abdelmagid O,Chengappa MM.Hybridization analysis of the gene encoding a hemolysin of Sreptococcus suis type2:evidence for the absence of the gene in some isolates[J].FEMS Microbiol Lett, 1999,181:113-21.
[37] Jacobs AA,Loefen PL,Van Den Berg AJ,et al. Identification,purification,and characterization of a thiol-a- ctivated hemolysin(suilysin) of Streptococcus suis[J].Infect Immun,1994,62(5):1742-8.
[38]方绍庆,陆承平.猪链球菌2型溶血素的化学修饰[J].微生物学报,2003,43(3):395-9.
[39] Tenenbaum T,Adam R,Eggelnpohler I,et,al.Strain-dependent disruption of blood-cerebrospinal fluid bar- rier by Sreptococcus suis in vitro[J]. FEMS Immunol Med Microbiol,2005,44(1):25-34.
[40] Vanier G,Segura M,Friedl P,et al.Invasion of porcine brain microvascular endothelial cells by Streptococ- cus suis serotype2[J].Infect Immun, 2004,72(3):1441-9.
[41] Jacobs AAC,van den Berg AJG,Baars JC.Protection of experimentally infected pigs by suilysin,thethiol- activated haemolysin of Streptococcus suis[J].Vet Rec, 1996,139:225-8.
[42] Norton PM,Rolph C,Ward PN.Epithelial invasion and cell lysis by virulent strains of Streptococcus suis is enhanced by the presence of suilysin[J].FEMS Immunol Med Microbiol,1999,26:25-35.
[43] Charland N,Nizet V,Rubens CE.Streptococcus suis serotype2 interactions with human brain microvascula rendothelial cells[J].Infect Immun,2000,68:637-43.
[44] Staats JJ,Plattner BL,Stewart GC.Presence of the Streptococcus suis suilysin gene and expression of MRP and EFcorrelates with high virulence in Streptococcus suis type2 isolates[J].Vet Microbiol,1999,70:201- 11.
[45] Flen AG,Bolitho S,Lindsay H.Generation and characterization of amutant of Streptococcussuis[J].Infect Immun,2001,69:2732-5.
[46] Quessy S,Busque P,Higgins R.Descroption of an albumin binding activity for Streptococcus suis seroty- pe 2[J].FEMS Microbiol Lett,1997,47:245-50.
[47] Haataja S,Tikkanen K,Hytonen J,et al.The Gal alpha 1-4Gal-binding adhesion of Streptococcus suis,a gram-positive meningitis-associated bacterium[J].Adv Exp Med Biol,1996,408:25-34.
[48] Smith HE,Buijs H,Wisselink HJ,et al.Selection of virulence-associated determinants of Streptococcus suis type2 by in vivo complementation[J].Infect Immun,2001,69(3):1961-6.
[49] Astridde G,Herma B,Robin V.Contribution of Fibronectin-Binding Protein to pathogenesis of Streptoco- ccus suis serotype2[J].Infect Immun,2002,70(3): 1319-25.
[50] Michael CF,Jose PC,Philip JW.Investigation of a Novel DNase of Streptococcus suis serotype2[J].Infect Immun,2004,72(2):774-81.
[51]田云,FrankM,陆承平.猪链球菌2型可能的毒力基因的发现[J].微生物学报,2004,44(5):613-6.
[52] Tang J, Wang C, Feng Y, et al. (2006) Streptococcal toxic shock syndrome caused by Streptococcus sui- s serotype 2. PLoS Med 3(e151):0668–76.
[53] Lindsay JA, Ruzin A, Ross HF, Kurepina N, Novick RP (1998) The gene for toxic shock toxin is carried by a family of mobile pathogenicity islands in Staphylococcus aureus. Mol Microbiol 29: 527–43.
[54] Chesney PJ (1983) Toxic-shock syndrome: a commentary and review of the characteristics of Staphyloc- occus aureus strains. Infection 11: 181–8.
[55] Stevens DL, Tanner MH, Winship J, Swarts R, Ries KM, et al. (1989) Severe group A streptococcal inf- ections associated with a toxic shock-like syndrome and scarlet fever toxin A. N Engl J Med 321: 1–7.
[56] Hacker J, Blum-Oehler G, Hochhut B, Dobrindt U (2003) The molecular basis of infectious diseases: pathogenicity islands and other mobile genetic elements. A review. Acta Microbiol Immunol Hung 50: 321–30.
[57] Ubeda C, Tormo MA, Cucarella C, Trotonda P, Foster TJ, et al. (2003) Sip, an integrase protein with ex- cision, circularization and integration activities, defines a new family of mobile Staphylococcus aureus pathogenicity islands. Mol Microbiol 49: 193–210.
[58] Fitzgerald JR, Monday SR, Foster TJ, Bohach GA, Hartigan PJ, et al. (2001) Characterization of a putati- ve pathogenicity island from bovine Staphylococcus aureus encoding multiple superantigens. J Bacteriol 183: 63–70.
[59] Cabello F (1997) Pathogenicity islands: important but not unique factors contributing to Salmonella viru- lence. Trends Microbiol 5: 431–2.
[60] Ochman H, Groisman EA (1996) Distribution of pathogenicity islands in Salmonella spp. Infect Immun 64: 5410–2.
[61] Brown JS, Gilliland SM, Holden DW (2001) A Streptococcus pneumoniae pathogenicity island encoding an ABC transporter involved in iron uptake and virulence. Mol Microbiol 40: 572–85.
[62] Matthew T. G. Holden, Heidi Hauser, Mandy Sanders,Thi Hoa Ngo, et al (2009) Rapid Evolution of Virulence and Drug Resistance in the Emerging Zoonotic Pathogen Streptococcus suis. PLoS ONE 4: e6072.
[63] Lévesque C, PichéL, Larose C, Roy PH. PCR mapping of integrons reveals several novel combinations of resistance genes[J]. Antimicrob Agents Chemother. 1995 Jan;39(1):185-91.
[64] Piddock LJ, Ricci V, McLaren I, et al. Role of mutation in the gyrA and parC genes of nalidixic-acid-re- sistant salmonella serotypes isolated from animals in the United Kingdom. J Antimicrob Chemother. 1998 Jun;41(6):635-41.
[65] Mason EJ,Wald ER,Bradley JS,et al.Macrolide resistance among middle ear isolates of Streptococcus p- neumoniae observed at eight United States pediatric centers:prevalence of M and MLSB phenotypes[J]. Pediatr Infect Dis J,2003,22:623-7.
[66] Bartelink AKM,E van Kregten.Streptococcus suis as threat to pig farmers and abattoir workers[J]. The lancet,1995,346(8991-8992):1707.
[67]蔡宝祥.家畜传染病学(第3版)[M].北京:中国农业出社,1996:115-8.
[68]陆承平,姚火春.科学进步与科学进展(下册)[M].南京:东南大学出版社,1999:339-43.
[69]姚火春,陈国强,陆承平.猪链球菌1998分离株病原特性鉴定[J].南京农业大学学报,1999,22(2):67-70.
[70] Perch B,Kjems E,Slot P,et al.Biochemical and serological properties of R,S and RS streptococci[J].Act a Pathol Microbiol Scand[B],1981,89(3):167-71.
[71] Hoffman LJ,Henderson LM.The signification of streptococcus suis in swine diseases:clinical,pathologic and bacteriologic date from a two year study[J].Proc Assoc Vet Lab Diagn,1985:201-10.
[72] Hommez J,Devriese LA,Henrichsen J,et al.Identification and characterization of streptococcus suis[J]. Vet Microbiol,1986,11(4):349-55.
[73] Sihvonen L,Kurl D N,Henrichen J. Streptococcus suis isolated from pigs in Finland[J].Act a Vet Scand, 1988,29(1):9-13.
[74] Higgins R,Gottschalk M,Mittal K R,et al. Streptococcus suis infecton in swine.A sixteen-month study[J]. Can J Vet Res,1990:54(1):170-3.
[75] Devriese LA,Ceyssens K,Hommez J,et al.Characteristics of different Streptococcus suis ecovars and des- cription of a simplified identification method[J].Vet Microbiol, 1991,26 (1-2):141-50.
[76] Tarradas C,Arenas A,Maldonado A,et al. Identification of Streptococcus suis isolated from swine:propos- al for biochemical parameters[J].J Clin Microbiol, 1994,32 (2):578-80.
[77] Oliver Goldmann,Gursharan S Chhatwal,Eva Medina.Contribution of Natural Killer Cells to the Pathoge- nesis of Septic Shock Induced by Streptococcus pyogenes in Mice[J].The Journal of Infectious Diseases Chicago,2005,191:1280-6.
[78] Manuel J Rodrguez Ortega,Nathalie Norais,Giuliano Bensi,et al.Characterization and identification of va- ccine candidate proteins through analysis of the group A Streptococcus surface proteome[J].Nature Biote- chnology New York,2006,24:191-7.
[79] Martinez G,Harel J,Higgins R,et al.Characterization of Streptococcus ag alactiae isolates of bovine andhuman origin by randomly amplified polymorphic DNA analysis[J]. Microbiol,2000,38:71-8.
[80] Martinez G,Harel J,Lacouture S, et al.Genetic diversity of Streptococcus suis serotypes 2 and 1/2 isolates recovered from carrier pigs in closed herds[J]. Can J Vet Res. 2002 Oct;66(4):240-8.
[81]李国生,杨晓阳,赵海金.中西医结合扑灭猪爆发性链球菌病[J].中国兽疫学杂志,2001,(1):19.
[82] Mwaniki CG,Robertson I D,Trott D J.etal.Clonal analysis and virulence of Australian isolates of Strepto- coccus suis type[J].Epidemiol Infect,1994, 113:321-34.
[83] Brousseau R,Hill J E, Prefontaine G, et al. Streptococcus suissero-types characterized by analysis of chaperonin 60 gene sequences[M].EnvMicrobiol, 2001.
[84]李明,何孔旺,陆承平.检测两种猪源链球菌抗体间接ELISA方法的筛选与应用[J].中国人兽共患病杂志,2005,(10):53-8.
[85]王海丽,徐公义,王长军等。链球菌2型人源分离株MRP单克隆抗体的制备与鉴定[J].中国兽医科学,2008(5):74-81.
[86] Betsy Foxman,Brenda Gillespie,Shannon D,etal.Incidence and Duration of Group B Streptococcus by se- rotype among Male and Female College Students Living in a Single Dormitory[J].American Journal of Epidemiology Oxford,2006,163:544.
[87]陈兴扶,徐瑞,尹燕博,等.用ELISA检测猪链球菌病血清抗体(初报)[J].中国动物检疫,1999,16(4):1-4.
[88]杨俊兴,金梅林,陈剑锋,等.猪链球菌2型抗体快速检测试纸条的制备及初步应用[R].北京:中国畜牧兽医学会2006学术年会,2006.
[89] Boye M,Feenstra A A,Tegtmeier C,etal.Detection of Streptococcus suis by insituhybridization, indirect immunofluorescence,and peroxidase antiper oxidas eassays informalin fixed,paraffin embedded tissues ections from pigs[J].Vet Diagn Invest,2000,12:224-32.
[90] Madsen L W,Boye M,Jensen H E.Anenzyme based in situ hybridization method for theident ification of Streptococcus suis[J].APMIS,2001,109:665-9.
[91] Roman Prymula,Pascal Peeters,Viktor Chrobok,etal.Pneumococcal capsular polysaccharides conjugated to protein D for prevention of Acute otitis media caused by both Streptococcus pneumoniaeandnon typa- ble Haemophilus influenzae:a randomized double blind efficacy study[J].Clin Otolaryngol,2006,31:435.
[92] Ulrike Haugwitz,Wiesia Bobkiewicz,Shan RuiHan,etal.Poreforming Staphylococcus aureus[alpha] toxin triggers epidermal growth factor receptor dependent proliferation[J].Cellular Microbiology,2006,8: 1591-600.
[93] Balakrishnan Prithiviraj,Harsh P Bais,Ajay KJ ha,etal.Staphylococcus aureus pathogenicity on Arabidop sisthaliana is mediated either by a direct effect of salicylic acid on the pathogen or by SA dependent.NP- R1 independent host responses[J].The Plant Journal,2005,42:417-32.
[94] Okwumabua O,Staats J,Chengappa M M.Detection of genomic heterogeneity in Streptococcus suis iso- lates by DNA restriction fragment length polymorphisms of rRNA genes(ribotyping)[J].J Clin Microbiol, 1995,33(4):968-72.
[95]倪艳秀,何孔旺,王继春,等.链球菌2型的快速检测[J].中国兽医学报,2002,22(5):473-6.
[96]倪宏波,欧阳素贞,王兴龙,等.利用随机扩增多态性DNA指纹技术对猪链球菌进行基因分型[J].中国兽医科技,2002,32(7):9-12.
[97]李国生,杨晓阳,赵海金.中西医结合扑灭猪爆发性链球菌病[J].中国兽疫学杂志,2001,(1):19.
[98]赵冉,孙建和,陆承平.多重PCR鉴定猪链球菌2型毒力菌株[J].上海交通大学学报(农业科学版), 2006,24(6):503-28.
[99]田云,Aarestrup F M,陆承平等.猪链球菌2型可能的毒力基因的发现[J].微生物学报,2004,44(5):613-6.
[100]郑腾.2型猪链球菌病检测技术的最新进展[J].福建畜牧兽医,2006,(增刊):23-6.
[101]郑峰,王长军,海攀等.猪链球菌基因芯片检测方法的研究[J].中国人兽共患病学报,2008,24(1):38-41.
[102] Gottschalk M,Segura M.The pathogenesis of the meningit is caused by Streptococcus suis: Unresolved questions[J].Vet Microbiol,2000,76:259-272.
[103] Smith HE,Rijnsburger M,Stockhofe-Zurwieden N, et al.Virulent strains of Streptococcus suis serotype 2 and highly virulent strains of Streptococcus suis serotype 1 can be recognized by a unique ribotype pro- file[J]. J Clin Microbiol. 1997 May;35(5):1049-53.
[104] Corinne Marois,La?titia Le Devendec,Marcelo Gottschalk, et al.Molecular characterization of Streptooc- ccus suis strains by 16S–23S intergenic spacer polymerase chain reaction and restriction fragment length polymorphism analysis[J].The Canadian Journal of Veterinary Research 2006;70:94–104.
[105]Achim Allgaier,Ralph Goethe,Henk J, et al.Relatedness of Streptococcus suis isolates of various serotyp- es and clinical backgrounds as evaluated by macrorestriction analysis and expression of potential virule- nce traits[J]. Journal of Clinical Microbiology Feb. 2001,p. 445–53.
[106] Florence B H,Marois C,Gott schalk M,et al.Geneti diversity of Streptococcus suis strains isolated from pigs and humans as revealed by pulsed-field gel electrophoresis[J].Clin Microbiol,2002,40:615-9.
[107] Wang S M,Smith H E,Rijnsburger M ,et al.Virulent strains of Streptococcus suis serotype 2 and highly virulent strains of Streptococcus suis serotype 1 can be recognized by a unique ribotype profile[J]. Clin Microbiol,2004,35:1049-53.
[108] Ana I V,Joaquin G,Carmen T, et al.Molecular typing by pulsed-field gel electrophoresis of Spanish ani- mal and human Listeria monocytogenes isolates [J].Appl Environ Microbiol,2001,67:5840-3.
[109] Cindy K.Y.Luey,Yiu Wai Chu,Terence K.M.Cheung, et al.Rapid pulsed-field gel electrophoresis proto- col for subtyping of Streptococcus suis serotype 2[J]. Journal of Microbiological Methods 68 (2007) 648– 50.
[110] Inmaculada Luque,Verena Blume,Carmen Borge, et al.Genetic analysis of Streptococcus suis isolates recovered from diseased and healthy carrier pigs at different stages of production on a pig farm [J].The Veterinary Journal.
[111] Enright MC, Spratt BG.Multilocus sequence typing[J]. Trends Microbiol 1999, 7:482-7.
[112] Samantha J. King, James A. Leigh, Peter J. Heath, et al. Development of a Multilocus Sequence Typ- ing Scheme for the Pig Pathogen Streptococcus suis: Identification of Virulent Clones and Potential Ca- psular Serotype exchange[J]. Journal of Clinical Microbiology,Oct.2002,p.3671-80.
[113] Chang B,Wada A,Ikebe T, et al.Characteristics of Streptococcus suis isolated from patients in Japan[J]. Jpn J Infect Dis. 2006 Dec;59(6):397-9.
[114] Rehm T,Baums CG,Strommenger B,Beyerbach M,Valentin-Weigand P,Goethe R.Amplified fragment length polymorphism of Streptococcus suis strains correlates with their profile of virulence-associated genes and clinical background[J].J Med Microbiol. 2007 Jan;56(Pt 1):102-9.
[115] Blume V, Luque I, Vela AI, et al. Genetic and virulence-phenotype characterization of serotypes 2 and 9 of Streptococcus suis swine isolates[J]. Int Microbiol. 2009 Sep;12(3):161-6.
[116] Kerdsin A, Oishi K, Sripakdee S, et al. Clonal dissemination of human isolates of Streptococcus suis serotype 14 in Thailand[J]. J Med Microbiol. 2009 Nov;58(Pt 11):1508-13. Epub 2009 Aug 6.