落叶松—杨栅锈菌生理小种鉴定
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摘要
落叶松-杨栅锈菌(Melampsora larici-populina Kleb.)又称青杨叶锈病,广泛分布于世界各杨树栽植区,可危害青杨派,黑杨派及其杂交杨,甚至胡杨等多种杨树,是杨树锈病中分布最广,危害最大的一种病害,在我国主要分布于东北、西北、华北及西南地区。
本论文主要对来自全国7个省市一定地域、不同年份、不同批次的15个落叶松-杨栅锈菌菌系进行了鉴别寄主反应型测定和IGS分析,得出如下结论:
(1)通过鉴别寄主反应型测定以及潜育期、产孢量观察,进一步明确了落叶松-杨栅锈菌在我国的生理小种分化。初步将供试的15个菌系中的13个归为4个生理小种,Th_(053)、Sb和Cj_(052)归1号生理小种CMLP_1;Ts_(06)、Gl_(051)、Hb_(05-1)和Sb_(052)归2号生理小种CMLP2;Qh_(063)、Gl_(052)、Hdao和Sb_(051)归3号生理小种CMLP_3;Bq和Qh_(061)归5号生理小种CMLP_5;By_(05-3)和Zs暂单独划为一个致病类型。
(2)IGS1扩增结果表明,所有供试的15个菌系IGS1的目的条带约1Kb均在同一位置,大小没有差异,供试菌系的IGS1高度保守,小种内、小种间没有差异。此研究更进一步证明了我国落叶松-杨栅锈菌确实存在着生理小种分化和变异,且菌株间的DAN多态性与菌株的地理来源没有明显的相关性,各菌株的致病性与DNA多态性之间也没有明显的相关性。
Melampsora larici-populina Kleb., is also called populus cathayana leaf rust, widely distributes in various poplar planting areas in the world, which do harm to Tacamabaca, Aigeros, and their hybrids, even to the Diversiform-leaved poplar.
It is a kind of plant disease, which distributes broadly, disserves furthest, mainly distributes in northeast, northwest, North China and the southwest in China.
In this paper, fifteen Melampsora larici-populina Kleb. isolates were researched through the respective test on host distinction and IGS analysis. The isolates came from certain regions of seven provinces or cities in different years and different lots in our country.
The main conclusions as following:
(1) Through the respective test on host distinction as well as observation as to in-cubation period and sporulation to further confirm physiological race differentiation of Melampsora larici-populina Kleb. in our country,13 out of 15 belonged to 4 physiological races respectively;Th_(053),Sb and Cj_(052) went to No.1 physiological race,CMLP1; Ts_(06),Gl_(051),Hb_(05-1) and Sb_(052) fell into No.2 physiological race,CMLP_2;Qh_(063),Gl_(052), Hdao and Sb_(051) into No.3 physiological race,CMLP_3;Bq and Qh_(061) to No.5 physiologic-al race,CMLP_5;and By_(05-3) and Zs into a pathogenesis type temporarily.
(2) The aimed bandings of IGS1 for the 15 strains was in the identical position and had no difference in size.The 15 strains IGS1 were highly conservative. There were no difference available in the physiological race, inter- physiological race.The research furtherly proved Melampsora larici-populina Kleb. had the physiological race differentiation in China, the DAN polymorphism and origination of the strains had no obvious relevance, the pathogenicity and DNA polymorphism also had no obvious relevance.
本论文主要对来自全国7个省市一定地域、不同年份、不同批次的15个落叶松-杨栅锈菌菌系进行了鉴别寄主反应型测定和IGS分析,得出如下结论:
(1)通过鉴别寄主反应型测定以及潜育期、产孢量观察,进一步明确了落叶松-杨栅锈菌在我国的生理小种分化。初步将供试的15个菌系中的13个归为4个生理小种,Th_(053)、Sb和Cj_(052)归1号生理小种CMLP_1;Ts_(06)、Gl_(051)、Hb_(05-1)和Sb_(052)归2号生理小种CMLP2;Qh_(063)、Gl_(052)、Hdao和Sb_(051)归3号生理小种CMLP_3;Bq和Qh_(061)归5号生理小种CMLP_5;By_(05-3)和Zs暂单独划为一个致病类型。
(2)IGS1扩增结果表明,所有供试的15个菌系IGS1的目的条带约1Kb均在同一位置,大小没有差异,供试菌系的IGS1高度保守,小种内、小种间没有差异。此研究更进一步证明了我国落叶松-杨栅锈菌确实存在着生理小种分化和变异,且菌株间的DAN多态性与菌株的地理来源没有明显的相关性,各菌株的致病性与DNA多态性之间也没有明显的相关性。
Melampsora larici-populina Kleb., is also called populus cathayana leaf rust, widely distributes in various poplar planting areas in the world, which do harm to Tacamabaca, Aigeros, and their hybrids, even to the Diversiform-leaved poplar.
It is a kind of plant disease, which distributes broadly, disserves furthest, mainly distributes in northeast, northwest, North China and the southwest in China.
In this paper, fifteen Melampsora larici-populina Kleb. isolates were researched through the respective test on host distinction and IGS analysis. The isolates came from certain regions of seven provinces or cities in different years and different lots in our country.
The main conclusions as following:
(1) Through the respective test on host distinction as well as observation as to in-cubation period and sporulation to further confirm physiological race differentiation of Melampsora larici-populina Kleb. in our country,13 out of 15 belonged to 4 physiological races respectively;Th_(053),Sb and Cj_(052) went to No.1 physiological race,CMLP1; Ts_(06),Gl_(051),Hb_(05-1) and Sb_(052) fell into No.2 physiological race,CMLP_2;Qh_(063),Gl_(052), Hdao and Sb_(051) into No.3 physiological race,CMLP_3;Bq and Qh_(061) to No.5 physiologic-al race,CMLP_5;and By_(05-3) and Zs into a pathogenesis type temporarily.
(2) The aimed bandings of IGS1 for the 15 strains was in the identical position and had no difference in size.The 15 strains IGS1 were highly conservative. There were no difference available in the physiological race, inter- physiological race.The research furtherly proved Melampsora larici-populina Kleb. had the physiological race differentiation in China, the DAN polymorphism and origination of the strains had no obvious relevance, the pathogenicity and DNA polymorphism also had no obvious relevance.
引文
[1] 余仲东,高爱琴,曹支敏.杨树抗锈性研究现状[J].西北植物学报,2004,24(6):1160-1164.
[2] 牛春山.陕西杨树.陕西科学技术出版社[M],1880,1-2.
[3] 赵天赐,陈章水.中国杨树集约栽培[M].北京:中国科学技术出版社,1994,1-59.
[4] 郑世锴,高瑞桐等.杨树丰产栽培与病虫害防治[M].北京:金盾出版社,1994,1-59.
[5] 中国科学院中国植物编辑委员会.中国植物志.第二十卷第二十分册[M].北京:科学出版社,1984, 1-78.
[6] 刘美青,李淑玲.杨树抗性研究的现状及展望[J].河南农业大学学报,1998,32(3): 253-257.
[7] 刘正楠.东北树木病害菌类图志[M].1981,5-16.
[8] 骆有庆 , 温俊宝 . 中国 “ 三北 ”009 项目区主要林木病虫害手册 [M]. 北京 : 中国林业出版社,1996,98-100.
[9] 孟繁荣,薛熠,黄永青.青杨叶锈病的研究[J].林业科技,1992,17(2):33-35.
[10] 尚衍重,郝秀贞,袁秀英.内蒙古的杨树栅锈菌[J].华北农学报,1990,5(2):86-92.
[11] 袁毅.我国杨树叶锈病菌种类的研究[J].北京林学院学报,1984,(1):48-82.
[12] 周仲铭,袁毅.杨树叶锈病研究概况[J].北京林学院学报.1985,(4):84-102.
[13] 孟繁荣,李国臣,石春玲等.抗青杨叶锈病杨树品种序列的筛选[J].林业科技,1996, 21(2):39-40.
[14] 尚衍重,郝秀贞,袁秀英.内蒙古的杨树栅锈菌[J].华北农学报,1990,5(2):86-92.
[15] 尚衍重 , 裴明浩 . 山杨叶锈病 (Melampsora larici-tremulae KLeb) 的研究 [J]. 东北林学院学报,1984,12(1):47-55.
[16] 袁毅.我国杨树叶锈病菌种类的研究[J].北京林学院学报,1984,(1):48-82.
[17] 孟繁荣,黄永青,倪乃华等.青杨叶锈防治指标的研究[J].东北林业大学学报, 1997,25(3):18-21.
[18] 潘彦平.落叶松-杨栅锈菌生理小种分化研究[D].陕西 杨陵.2002.
[19] 裴明浩,尚衍重.青杨叶锈病(Melampsora larici-populina KLeb)的研究[J].东北林学院学报,1984,12(2):40-48.
[20] 田呈明,李振岐,康振生.落叶松-杨栅锈菌遗传分化的 RAPD 分析[J].林业科学,2000,36(5):54-58.
[21] 吴晓芹.林木病原真菌的群体遗传分化研究[J].林业科学研究,2000,13(4):423-430.
[22] 伊藤:《日本菌类志》二卷二号,东京养贤堂,1938.
[23] 袁嗣令.中国乔灌木病害[M].北京:中国林业出版社,1998,110-112.
[24] 苑健羽.落叶松真菌病害[M].北京:科学出版社,1990,89.
[25] Pinon J. Management of poplar disease. Eur J For Path 1984,14:415-425.
[26] Richard C. Hamelin, Richard S.Ferriss,and Louis Shain. Prediction of poplar leaf epidemics from a leaf-disk assa[J].Can.J.Res., 1994,24:2085-2088.
[27] Steenackers J, Steenackers M, Steenackers V. Diseases of poplars effects on growth and wood duality[J]. Bulletin Trimestriel Centre de Populiculture du Hainaut, Cahiers-Techniques-de-1. Objectif1,1995(3):4-20.
[28] Cheng—Ming Tian.Yan-Zhong Shang.Jian—Yun Zhuang. Morphological and molecular phylogenetic analysis of Melampsora species on poplars in China.Mycoscience.2004,45: 56-66.
[29] 任本权.落叶松-杨栅锈菌遗传多样性分析[D].陕西 杨陵.2003.
[30] 田呈明,李振岐,康振生.青杨叶锈病研究进展[J].西北林学院学报,1999,14(2):81-88.
[31] Bart,A.Thielges,John,C.Adams.Genetic variation and heritability of melampsora leaf rust resistance in Cottonwood[J].Forest science,21(3):278.
[32] Burdon,J.J.,and Roberts,J.K.The population genetic of the rust fungus melampsora lini as revealed by pathogenicity,isozyme and RFLP markers[J].Plant pathologu,1995,44:270-278.
[33] Cervera,M.T.Gusmao,J.,steenackers,M.,Peleman,J.Identification of AFLP molecular marker for re-sistance against Melampsora larici-populina[J].Theor.Appl.Genet.,1996b,3:733-737.
[34] Chandrashekar,M.,Heather,W.A.Reaction of poplar clines to physiologic of Melampsora larici-populina[J].Euphytica,1980,29:401-407.
[35] Chiba Osamu,Uredospores of the poplar leaf rust,Melampsora larici-populina Kleb.,as a sources of primary infection.Journal of the Japanese forestry society,1960,42(11):406-410.
[36] Heather W A,Sharma J K.Physiologic specialization in hyper parasitism of races of Melampsora larici-populina by isolates of Cladosporium tenuissimum[J].Eur.J.For.Path.,1987,17:185-188.
[37].郝俊贞.青杨叶锈病在转主寄主落叶松发病规律及化学防治[J].内蒙古林业科技,1998,(4):44-47.
[38] 高雅.松杨栅锈菌(Melampsora larici-populina KLeb) 的侵染循环和抗锈树种的筛选[J].东北林学院学报,1981,(3):10-18.
[39] 张山林.青杨叶锈病损失量估计研究[J].甘肃林业科技,1990,(2):29-33.
[40] 曹支敏,李振岐.秦岭锈菌[M].中国林业出版社.北京,1999.
[41] Van Vloten H. Hybridization experiment with races of Melampsora larici-populina Kleb.[J].Tij-dschr.Pl.Ziekt,1949,55:196-209.
[42] Pinon J,Van Dam BC,et al.Tow pathogenic races of Melampsora larici-populina Kleb. in nort-h-western Europe[J].Eur.J.For.Path.1987,17:47-53.
[43] Pinon J,Peulon V.Evidence of third physiological race of Melampsora larici-populina Kleb. in Europe[J].Cryptogamie Mycologie,1989,10(2):95-106.
[44] Pinon J.Variability in the genus Populus in sensitivity to Melampsora rusts.[J].Silvae Genetica,1992,41(1):25-34.
[45] Landmann G,Barthod C.Lasantedes forets health in France,1994[J]. Revue-Forestiere-Francaise,
[46] 曹支敏,李振岐,胡景江.落叶松-杨栅锈菌生理分化研究[J].西北林学院学报,1998,19(1):53-57.
[47] 潘彦平.落叶松-杨栅锈菌(Melampsora larici-populina Kleb.)生理小种分化及其寄主抗病性研究[D].西北农林科技大学:2002.
[48] 任本权,曹支敏,潘彦平等.落叶松-杨栅锈菌致病性分化研究[J].西北林学院学报,2003,18(2):51-54.
[49] 曹支敏,余仲东,潘彦平等.中国落叶松-杨栅锈菌生理小种分化[J].植物病理学报,2005,35(2):184 -186.
[50] 陈建珍.落叶松-杨栅锈菌DNA多态性和同工酶分析[D].西北农林科技大学:2006.
[51] 雷天刚.甘蓝型黄籽油菜 SSR 和同工酶标记的遗传多样性及指纹图谱[D].西南农业大学:2005.
[52] 韩冰,曹远银,蔺瑞明等.分子标记技术在小麦条锈菌研究中的应用[J].中国科技信息,2006,7:126 -128.
[53] Botstein D,White R,Davis R. Construction of a genetics linkage map in man using restriction fragment length polymorphisms[J].Journal of Human,1980,32: 314-331.
[54] 董甜,张惠文,张粤等.长白山四种赤杨丛枝菌根真菌侵染多样性的巢式PCR-RFLP[J].应用生态学报,2006,17(10):1796-1800
[55] 秦振宇,吴绍熙,吕桂霞等.PCR-RFLP鉴别念珠菌、曲霉和隐球菌的探讨[J]. J Clin Dermatal,2000,29(2):76-78.
[56] 赵勇,李武,周志华等.应用PCR-RFLP及PCR-TGGE技术监测农田土壤微生物短期动态变化[J].南京农业大学学报,2005,28(3):53-57.
[57] 秦旭升,刘学敏,于荣利.运用PCR-RFLP标记检测南瓜疫病菌[J].东北农业大学学报,2003,34(4): 368-371.
[58] Williams JG K,Kubelik AR,Livak KJ. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers[J].Nucleic Acids Research,1990,18:6231-6235.
[59] Hu J,Quiros CF.Identification of broccoli and cauliflower cultivars with RAPD marker[J].Plant Cell Reports,1991,10:505-511.
[60] Torres AM.Identifying tore cultivars using random amplified polymorphic DNA marker[J].Hortscience,1993,28:333-334.
[61] 冯洁,孙文姬,石磊研等.中国棉花枯萎病菌生理小种的RAPD分析[J].菌物系统,2000,19(2):45～50
[62] 单卫星,陈受宜,吴立人等.中国小麦条锈菌流行小种的RAPD分析[J].中国农业科学,1995,28(5):1～7.
[63] Grajal-Martin M J. Use of random amplified polymorphic DNA(RAPD) to characterize race 2of Fusarium oxysporium f. sp.pisi[J].Phytopathology,1993,(83):612～614
[64] Nicholson P,KezanoorH Z,苏海.用RAPD分析 DNA 指纹鉴别玉米小斑病菌O、C、T小种[J].植物病理学报,1993,(23):114.
[65] 甘丽萍,王生荣.RAPD标记在植物病原真菌遗传多样性及生理分化研究中的应用[J].甘肃农业大学学报,2004,39(1):72-76.
[66] 陈作红, 张志光, 张天晓. 鹅膏菌属真菌 RAPD 分析及亲缘关系的研究[J]. 菌物系统, 2000, 19(2): 51～55
[67] Vos P,Hogers R,Bleeker M et al.AFLP:a new technique for DNA fingerprinting[J].N uceic Acids Research,1995,23:4407-4414.
[68] 关德军.AFLP技术原理及其在植物研究中的应用[J].安徽农业科学,2006,34(15):3625-3626.
[69] 云晓鹏,李子钦,赵存虎.AFLP在我国植物病原真菌分子生物学研究中的应用[J].2006,10:9-12.
[70] 郑文明,刘峰,康振生,等.中国小麦条锈菌主要流行菌系的AFLP指纹分析[J].自然科学进展,2000, 10(6):532-537.
[71] 张增艳,曾祥艳,林志珊,等.小麦新种质YW243抗条锈病新基因的AFLP标记[J].中国农学通报,2005,21(12):56-59.
[72] 段会军,姬惜珠,张彩英,等.几种瓜类枯萎病菌专化型的AFLP分析[J].河北农业大学学报,2005,2(5):71-74.
[73] Greaves DR,Patient RK.(AT)n is an interspersed repeat in the Xenopus genome[A].The EMBOJournal,1985,4:2617-2626.
[74] Groppe K,Sanders L,Wiemken A. et al.A microsatellit marker for studying the ecology and di-versity of fungal endophytes(Epichloe spp.)in grasses[A].Apllied and Environmental Microbiolo-gy,1995,61:3943-3949.
[75] Kaye C,Milazzo J,Rozenfeld S et al.The development of simple sequence repeat markers for Magnaporthe grisea and their Integration into an established genetic linkage map[A].FungalGenetics and Biology,2003,40:207-214.
[76] Wet J,Burgess T,Slippers B et al.Multiple gene genealogies and Microsatellite markers reflect relationships between morphotypes of Sphaeropsis sapinea and distinguish a new species of Diplodi-a[A].Mycological Research,2003,107:557-566.
[77] Burgess T,Wingfiele MJ,Wingfield BW.Simple sequence repeat markers distinguish among mor-photypes of Sphaeropsis sapinea[A].Applied and Environmental Microbiology,2001,67:354-362.
[78] Hennequin C,Thieny A,Tichard GF et al.Microsatellite typing as a new tool for Identificationof Saccharomyces cerevisiae strains[A].Journal of Clinical Microbiology,2001,39:551-559.
[79] 张瑞颖.香菇菌株多相鉴定鉴别技术研究[D].中国农业大学:2004.
[80] Dunham SM,Dell TE,Molina R.Analysis of nrDNASequences and microsatellite allele frequencies reveals a Cryptic chanterelle species Cantharelluscus cascadensis sp.nov.from the American Pacific Northwest[A].Mycological Research,2003,107:1163-1177
[81] Hibbet DS,Fukumasa NY,Tsunoda A,Donoghue MJ.PHylogenetic diversity in shiitake inferred from nucear Ribosomal DNA sequences[A].Mycologia,1995,87:618-638.
[82] Guidot A,Lumini E,Debaud JC,Marmeisse R.The Nuclear Ribosomal DNA Intergenic Spacer as a Target Sequence To Study Intraspecific Diversity of the Ectomycorrhizal Basidiomycete Hobeloma cylindrosporum Directly on Pinus Root Systems[A].Apllied and Environmental Micr-obiology,1999,65:903-909.
[83] Baldridge GD,Dalton MW,Fallon AM.Is higherorder structure conserved in eukaryotic ribosomal DNA intergenic spacers?[A].Journal of Molecular Evolution,1992,35:514-523.
[84] Albee SR,Mueller GM,Kropp BR.Polymorphisms in the large intergenic spacer of the nuclear ribosomal repeat identify Laccaria proxima strains[A].Mycologia,1996,88:970-976.
[85] Ganley ARD,Barry S. Extraordinary Ribosomal Spacer Length Heterogeneity in a Neotyphodiu-m Endophyte Hybrid:Implications for Concerted Evolution[A].Genetics,1998,150:1625-1637.
[86] Szostak JW,Wu J.Unequal crossing over in the ribosomal DNA of Saccharomyces cemvisiae[A].Nature,1980,284:426-430.
[87] JacksonJ A,Fink GR.Gene conversion between duplicated genetic elements in yeast[A].Nature,1981,292:306-311.
[88] Mwenje E,Wingfield BD,Coetzee MP,Wingfield MJ. Molecular characterisation of Arrndlaria species from Zimbabwe[A].Mycological Research,2003,107:291-296
[89] Pramateftaki PV,Antoniou PP Types MA.The complete DNA sequence of the nuclear ribosom-al RNA gene complex of verticillium dahliae:intraspecific heterogeneity within the intergenic spacerregion[A].Fungal Genetics and Biology,2000,29:19-27.
[90] Sugita T,Kodama M,Saito M,Ito T,Kato Y,Tsuboi R,Nishikawa A.Sequence diversity of the intergenic spacer region of the rRNA gene of Malassezia globosa colonizing the skin of patients with atopic dermatitis and healthy individuals[A].Journal of Clinical Microbiology,2003,41:30 22-302.
[91] 张金霞,黄晨阳,张瑞颖等.中国栽培白灵侧耳的RAPD和IGS分析[J].菌物学报,2004,23(4):514-519.
[92] 黄晨阳,张金霞,郑素月等.刺芹侧耳(Pleurotuseyngii)rDNA的IGS2多样性分析[J].农业生物技术学报,2005,13(5):592-595.
[93] 邹容.天麻共生蜜环菌及同属真菌的基因系谱学及药用成分的研究[D].贵州大学:2005.
[94] Pei, MH,Whelan,MJ,Halford,NG. And Royle,DJ.Distinction between stem-and leaf-infecting forms of Melampsora rust on Salix viminalls using RAPD marks[A].Mycol.res.,1997,101(1):7-10.
[95] 余仲东,刘小勇,曹支敏.松杨栅锈菌的ITS序列和微卫星分析[J].中国农业科学,2006,39(6):1159-1165.
[96] Pino J,Frey P.Structue of Melampsora larici-populina populations on wild and cultivated popla-r[A].Eur.J.Plant Pathol., 1997,103(2):159-173.
[97] Lefevre F,Pichot C and Pionon J.Intra—and interspecific inheritance of some components of t-he resistance to leaf rust (Melampsora larici populina Kleb.)in poplars[A].Theor Appl.Genet,1994,88:501-507.
[98] Pinon J,Frey P.Miots, Villar M.The problem of durable resistance in the case of poplar rust(Melampsora larici-populina)[A].Durable Resistance Symposium,Wageningen(NL),2000.
[99] inon. J Reaction of main poplar clones to rust,particularly to Melampsora larici-populina[A].Revue Forestiere Francaise,1991,43(4):301-308.
[100] Picjot.Ch,Teissier Du Cros E. Susceptibility of P.deltoids Bartr to Melampsora larici-populinaand Melampsora alli-populina I.Quantitative analysis of a 6×6 factorial mating design[A].Siva-e Genetica,1993,42(4-5):179-187.
[101] Lefever F.Goue Mourier M C,Faivre Rampant P,etal.A single gene cluster controls in compati-bility and partial resistance to various Melampsora larici-populina races in hybrid poplars[A].Phytopathology,1998,88(2):156-163.
[102] Chiba Osamu,Study on the variation susceptibility and the nature of resistence of poplars to the leaf rust caused by Melampsora larici-populina Kleb.,Bullitin of the Government forest Experiment Station,No.166,Toyo,Japan.1994.
[103] Wilkinson,A.G.and Van Karraakyenoord,C.W.S.,Susceptibility classification of poplar clone to Melampsora rust in New Zealand[A].FAO/IPC Beograd,1975,19(4):942-952.
[104] 田呈明.落青杨叶锈病组织病理学和细胞学及病原菌遗传分化研究[D].杨陵:西北农林科技大学博士论文,2002:6
[105] 陈建珍,曹支敏,樊军锋.杨树叶锈病寄主抗性调查[J].西北林学院学报,2005,20(1):153-155.
[106] Siwecki,R.et al.Resistance mechanisms in interactions between poplars and rust.Resistance to d-isease and pests in forest trees[A].Wageningen,Centre for agricultural publishing and ducomentation,1982,130-142.
[107] Suzuki,K.et al.Studies on the susceptibility to poplar leaf rust influenced by the different nutr-ient condition 3 changes in free amino acid content[A].Journal of the Japanese Forestry Socie-t,1974,56(7):235-238.
[108] 胡 景 江 , 曹 支 敏 , 文 建 雷 . 落 叶 松 - 杨 栅 锈 菌 寄 主 抗 病 性 的 研 究 [J]. 林 业 科 学 研 究 , 2000,13(专):114-118.
[109] Szymanski M, et al. Nucleic Acids Res,2000,28:166-167.
[110] Einsele H, et al.J Clin Microbial,1997,35:1353-1360.
[111] 李振歧,商鸿生.小麦锈病及其防治[M].上海:上海科技出版社报,1989,211-229.
[112] 王廷华,景强.PCR理论与技术[M].北京:科学出版社,2005.
[113] 周俊宜.分子生物学基本技能和策略[M].北京:科学出版社,2003.
[114] 陈毓荃.生物化学实验方法和技术[M].北京:科学出版社,2002.
[115] 余 仲 东 , 高 爱 琴 , 曹 支 敏 . 锈 菌 夏 孢 子 DNA 的 微 量 快 速 提 取 方 法 [J]. 生 物 技 术 通讯,2005,16(1):48-50.
[116] 朱衡,瞿峰,朱立煌.利用氯化苄提取适于分子生物学分析的真菌DNA[J].真菌学报, 1994,13(1):34-40.
[117] 卢圣栋.现代分子生物学实验技术[M].北京:中国协和医科大学出版社,1999.
[118] 王国英.基因工程实验技术[M].北京:中国农业出版社,1997.
[119] 朱平.PCR 基因扩增实验操作手册[M].北京:中国科学技术出版社,1992.
[120] White T J,B runs TD,Lee SB,et al.Amp lif ication and direct sequencing of fungal ribosomal RNA genes for phylogenetics[A].Innis M A,Gelfand D H,Sninsky J J, et al PCR Protocols:A Guide to Methods and Applications[M ].New York: Academic Press,1990,315-321.
[121] Anderson J,Stasovski E.Molecular phylogeny of northern hemisphere species of Armillaria[A].Mycologia,1992,84(4):505~51.
[122] Timothy Y.James,Jean-Marc Moncalvo,Sean Li and Rytas Vilgalys.Polymorphism at the Ribos-omal DNA Spacers and Its Relation to Breeding Structure of the Widespread Mushroom Schizophyl-lum commune[J].Genetic,2001,157:149-161.
[123] Timothy Y.James and Rytas Vilgalys.Abundance and diversity of Schizophyllum commune spore clouds in the Caribbean detected by selective sampling[J].Molecular Ecology,2001,10:471-479.
[124] 李竟雄,宋同明著.植物细胞遗传学[M].北京:科学出版社,1997,1-51.
[125] 沈法富,刘风珍,于元杰.分子标记在植物育种中的应用[J].山东农业大学学报,28(1):83-91.
[126] 陆朝福,朱立煌.植物育种中分子标记辅助选择[J].生物工程进展,1995,15(4):11-17.
[127] 曹丽华.中国小麦条锈菌生理小种分子标记的研究[D].中国杨凌.2003.
[128] 金海国,姜成国,白红星等.分子遗传标记及其分析技术的研究进展[J].延边大学农学学报,2002,24(1):55—59.
[129] 梁宏,彭友良,张国珍等.腥黑粉菌属3中检疫性真菌rDNA-IGS区的扩增及其序列分析[J].植物病理学报,2006,36(5):407-412.
[130] 秦国夫,赵俊,田淑敏.北半球高卢蜜环菌的遗传多样性与分子鉴定[J].林业科学,2001,37(2):61 -69.
[131] 秦国夫,J.HANTULA.同宗配合蜜环菌与欧美异宗配合蜜环菌狭义种的分子系统学关系[J].菌物系统,2002,21(3):346-355.
[134] Vos P,Hogers R,Bleeker M et al.AFLP:a new technique for DNA fingerprinting[J].N uceic Acids
[135] Williams JGK,Hanafey MK,Rafalski JA.Genetic analysis using random amplified polymorphic DNA markers[J].Methodsin Enzymology,1993,218:704-740.Research,1995,23:4407-4414.
[136] Richards RI,Sutherland GR.Dynamic mutations:a new class of mutations causing human disea-se[J].Cell,1992,70:709-712.
[137] Wostemeyer J,Kreibich A.Repetitive DNA elements in fungi (Mycota):impact on genomic architecture and evolution[A].Current genetics,2002,41:189-198.
[138] Toth G,Gaspari Z,Jurka J.Mecrosatellites in diferent eukaryotic genomes:survey and analysis[J].Genome research,2000,10:967-981.
[139] 李传道.森林病害流行与治理[M].中国林业出版社,北京,1995:36-48.
[140] 方中达.植病研究法[M].北京:中国农业出版社,1998,361-362.
[141] 余仲东,张星耀,曹支敏.真菌核糖体基因间隔区研究概况[J].西北林学院学报,2000,15(2):107 -112.
[142] 余仲东,梅华全,曹支敏等.杨树锈病的PCR检测[J].西北林学院学报,2005,20(3):131-132.
[143] 林晓民,李振岐,王少先.真菌rDNA的特点及在外生菌根菌鉴定中的应用[J].西北农业学报,2005,14(2):120-125.
[144] 万兵,闫杰,季明春等.一种优化的PCR方法——降落PCR扩增目的基因[J].江苏临床医学,2002,6(2):127-129.
[145] 张贵星,袁保梅,许培荣等.一种高特异的改良降落PCR[J].生命科学研究,2002,6(4):314-317.
[146] 魏明,曹支敏,田呈明.中国落叶松杨栅锈菌4个流行小种的RAPD标记[J].2007,35(18):5366-5367, 5392.
[147] 金广峰,杨彦玲.环境因子对青杨锈病发生的影响[J].河北林业科技,2005,6:11.
[148] 郑淑霞,王占林.美洲黑杨×青杨杂交无性系抗锈病能力分析[J].青海农林科技,2004.42-43.
[149] 李瀚,曾汉清.青杨叶锈病流行规律及防治技术[J].陕西林业科技,1999,3:51-53.
[150] 李淑玲,侯建治,马浩.毛白杨不同无性系苗木抗锈性的初步研究[J].河南农业大学学报,1994,28(2):124-127.
[151] 祁承德,周卫芬,吴洪源等.落叶松杨锈病Melampsora larici-populina Kleb.发生规律及苗木损失量研究[J].青海农林科技,1995,2:27-30.
[2] 牛春山.陕西杨树.陕西科学技术出版社[M],1880,1-2.
[3] 赵天赐,陈章水.中国杨树集约栽培[M].北京:中国科学技术出版社,1994,1-59.
[4] 郑世锴,高瑞桐等.杨树丰产栽培与病虫害防治[M].北京:金盾出版社,1994,1-59.
[5] 中国科学院中国植物编辑委员会.中国植物志.第二十卷第二十分册[M].北京:科学出版社,1984, 1-78.
[6] 刘美青,李淑玲.杨树抗性研究的现状及展望[J].河南农业大学学报,1998,32(3): 253-257.
[7] 刘正楠.东北树木病害菌类图志[M].1981,5-16.
[8] 骆有庆 , 温俊宝 . 中国 “ 三北 ”009 项目区主要林木病虫害手册 [M]. 北京 : 中国林业出版社,1996,98-100.
[9] 孟繁荣,薛熠,黄永青.青杨叶锈病的研究[J].林业科技,1992,17(2):33-35.
[10] 尚衍重,郝秀贞,袁秀英.内蒙古的杨树栅锈菌[J].华北农学报,1990,5(2):86-92.
[11] 袁毅.我国杨树叶锈病菌种类的研究[J].北京林学院学报,1984,(1):48-82.
[12] 周仲铭,袁毅.杨树叶锈病研究概况[J].北京林学院学报.1985,(4):84-102.
[13] 孟繁荣,李国臣,石春玲等.抗青杨叶锈病杨树品种序列的筛选[J].林业科技,1996, 21(2):39-40.
[14] 尚衍重,郝秀贞,袁秀英.内蒙古的杨树栅锈菌[J].华北农学报,1990,5(2):86-92.
[15] 尚衍重 , 裴明浩 . 山杨叶锈病 (Melampsora larici-tremulae KLeb) 的研究 [J]. 东北林学院学报,1984,12(1):47-55.
[16] 袁毅.我国杨树叶锈病菌种类的研究[J].北京林学院学报,1984,(1):48-82.
[17] 孟繁荣,黄永青,倪乃华等.青杨叶锈防治指标的研究[J].东北林业大学学报, 1997,25(3):18-21.
[18] 潘彦平.落叶松-杨栅锈菌生理小种分化研究[D].陕西 杨陵.2002.
[19] 裴明浩,尚衍重.青杨叶锈病(Melampsora larici-populina KLeb)的研究[J].东北林学院学报,1984,12(2):40-48.
[20] 田呈明,李振岐,康振生.落叶松-杨栅锈菌遗传分化的 RAPD 分析[J].林业科学,2000,36(5):54-58.
[21] 吴晓芹.林木病原真菌的群体遗传分化研究[J].林业科学研究,2000,13(4):423-430.
[22] 伊藤:《日本菌类志》二卷二号,东京养贤堂,1938.
[23] 袁嗣令.中国乔灌木病害[M].北京:中国林业出版社,1998,110-112.
[24] 苑健羽.落叶松真菌病害[M].北京:科学出版社,1990,89.
[25] Pinon J. Management of poplar disease. Eur J For Path 1984,14:415-425.
[26] Richard C. Hamelin, Richard S.Ferriss,and Louis Shain. Prediction of poplar leaf epidemics from a leaf-disk assa[J].Can.J.Res., 1994,24:2085-2088.
[27] Steenackers J, Steenackers M, Steenackers V. Diseases of poplars effects on growth and wood duality[J]. Bulletin Trimestriel Centre de Populiculture du Hainaut, Cahiers-Techniques-de-1. Objectif1,1995(3):4-20.
[28] Cheng—Ming Tian.Yan-Zhong Shang.Jian—Yun Zhuang. Morphological and molecular phylogenetic analysis of Melampsora species on poplars in China.Mycoscience.2004,45: 56-66.
[29] 任本权.落叶松-杨栅锈菌遗传多样性分析[D].陕西 杨陵.2003.
[30] 田呈明,李振岐,康振生.青杨叶锈病研究进展[J].西北林学院学报,1999,14(2):81-88.
[31] Bart,A.Thielges,John,C.Adams.Genetic variation and heritability of melampsora leaf rust resistance in Cottonwood[J].Forest science,21(3):278.
[32] Burdon,J.J.,and Roberts,J.K.The population genetic of the rust fungus melampsora lini as revealed by pathogenicity,isozyme and RFLP markers[J].Plant pathologu,1995,44:270-278.
[33] Cervera,M.T.Gusmao,J.,steenackers,M.,Peleman,J.Identification of AFLP molecular marker for re-sistance against Melampsora larici-populina[J].Theor.Appl.Genet.,1996b,3:733-737.
[34] Chandrashekar,M.,Heather,W.A.Reaction of poplar clines to physiologic of Melampsora larici-populina[J].Euphytica,1980,29:401-407.
[35] Chiba Osamu,Uredospores of the poplar leaf rust,Melampsora larici-populina Kleb.,as a sources of primary infection.Journal of the Japanese forestry society,1960,42(11):406-410.
[36] Heather W A,Sharma J K.Physiologic specialization in hyper parasitism of races of Melampsora larici-populina by isolates of Cladosporium tenuissimum[J].Eur.J.For.Path.,1987,17:185-188.
[37].郝俊贞.青杨叶锈病在转主寄主落叶松发病规律及化学防治[J].内蒙古林业科技,1998,(4):44-47.
[38] 高雅.松杨栅锈菌(Melampsora larici-populina KLeb) 的侵染循环和抗锈树种的筛选[J].东北林学院学报,1981,(3):10-18.
[39] 张山林.青杨叶锈病损失量估计研究[J].甘肃林业科技,1990,(2):29-33.
[40] 曹支敏,李振岐.秦岭锈菌[M].中国林业出版社.北京,1999.
[41] Van Vloten H. Hybridization experiment with races of Melampsora larici-populina Kleb.[J].Tij-dschr.Pl.Ziekt,1949,55:196-209.
[42] Pinon J,Van Dam BC,et al.Tow pathogenic races of Melampsora larici-populina Kleb. in nort-h-western Europe[J].Eur.J.For.Path.1987,17:47-53.
[43] Pinon J,Peulon V.Evidence of third physiological race of Melampsora larici-populina Kleb. in Europe[J].Cryptogamie Mycologie,1989,10(2):95-106.
[44] Pinon J.Variability in the genus Populus in sensitivity to Melampsora rusts.[J].Silvae Genetica,1992,41(1):25-34.
[45] Landmann G,Barthod C.Lasantedes forets health in France,1994[J]. Revue-Forestiere-Francaise,
[46] 曹支敏,李振岐,胡景江.落叶松-杨栅锈菌生理分化研究[J].西北林学院学报,1998,19(1):53-57.
[47] 潘彦平.落叶松-杨栅锈菌(Melampsora larici-populina Kleb.)生理小种分化及其寄主抗病性研究[D].西北农林科技大学:2002.
[48] 任本权,曹支敏,潘彦平等.落叶松-杨栅锈菌致病性分化研究[J].西北林学院学报,2003,18(2):51-54.
[49] 曹支敏,余仲东,潘彦平等.中国落叶松-杨栅锈菌生理小种分化[J].植物病理学报,2005,35(2):184 -186.
[50] 陈建珍.落叶松-杨栅锈菌DNA多态性和同工酶分析[D].西北农林科技大学:2006.
[51] 雷天刚.甘蓝型黄籽油菜 SSR 和同工酶标记的遗传多样性及指纹图谱[D].西南农业大学:2005.
[52] 韩冰,曹远银,蔺瑞明等.分子标记技术在小麦条锈菌研究中的应用[J].中国科技信息,2006,7:126 -128.
[53] Botstein D,White R,Davis R. Construction of a genetics linkage map in man using restriction fragment length polymorphisms[J].Journal of Human,1980,32: 314-331.
[54] 董甜,张惠文,张粤等.长白山四种赤杨丛枝菌根真菌侵染多样性的巢式PCR-RFLP[J].应用生态学报,2006,17(10):1796-1800
[55] 秦振宇,吴绍熙,吕桂霞等.PCR-RFLP鉴别念珠菌、曲霉和隐球菌的探讨[J]. J Clin Dermatal,2000,29(2):76-78.
[56] 赵勇,李武,周志华等.应用PCR-RFLP及PCR-TGGE技术监测农田土壤微生物短期动态变化[J].南京农业大学学报,2005,28(3):53-57.
[57] 秦旭升,刘学敏,于荣利.运用PCR-RFLP标记检测南瓜疫病菌[J].东北农业大学学报,2003,34(4): 368-371.
[58] Williams JG K,Kubelik AR,Livak KJ. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers[J].Nucleic Acids Research,1990,18:6231-6235.
[59] Hu J,Quiros CF.Identification of broccoli and cauliflower cultivars with RAPD marker[J].Plant Cell Reports,1991,10:505-511.
[60] Torres AM.Identifying tore cultivars using random amplified polymorphic DNA marker[J].Hortscience,1993,28:333-334.
[61] 冯洁,孙文姬,石磊研等.中国棉花枯萎病菌生理小种的RAPD分析[J].菌物系统,2000,19(2):45～50
[62] 单卫星,陈受宜,吴立人等.中国小麦条锈菌流行小种的RAPD分析[J].中国农业科学,1995,28(5):1～7.
[63] Grajal-Martin M J. Use of random amplified polymorphic DNA(RAPD) to characterize race 2of Fusarium oxysporium f. sp.pisi[J].Phytopathology,1993,(83):612～614
[64] Nicholson P,KezanoorH Z,苏海.用RAPD分析 DNA 指纹鉴别玉米小斑病菌O、C、T小种[J].植物病理学报,1993,(23):114.
[65] 甘丽萍,王生荣.RAPD标记在植物病原真菌遗传多样性及生理分化研究中的应用[J].甘肃农业大学学报,2004,39(1):72-76.
[66] 陈作红, 张志光, 张天晓. 鹅膏菌属真菌 RAPD 分析及亲缘关系的研究[J]. 菌物系统, 2000, 19(2): 51～55
[67] Vos P,Hogers R,Bleeker M et al.AFLP:a new technique for DNA fingerprinting[J].N uceic Acids Research,1995,23:4407-4414.
[68] 关德军.AFLP技术原理及其在植物研究中的应用[J].安徽农业科学,2006,34(15):3625-3626.
[69] 云晓鹏,李子钦,赵存虎.AFLP在我国植物病原真菌分子生物学研究中的应用[J].2006,10:9-12.
[70] 郑文明,刘峰,康振生,等.中国小麦条锈菌主要流行菌系的AFLP指纹分析[J].自然科学进展,2000, 10(6):532-537.
[71] 张增艳,曾祥艳,林志珊,等.小麦新种质YW243抗条锈病新基因的AFLP标记[J].中国农学通报,2005,21(12):56-59.
[72] 段会军,姬惜珠,张彩英,等.几种瓜类枯萎病菌专化型的AFLP分析[J].河北农业大学学报,2005,2(5):71-74.
[73] Greaves DR,Patient RK.(AT)n is an interspersed repeat in the Xenopus genome[A].The EMBOJournal,1985,4:2617-2626.
[74] Groppe K,Sanders L,Wiemken A. et al.A microsatellit marker for studying the ecology and di-versity of fungal endophytes(Epichloe spp.)in grasses[A].Apllied and Environmental Microbiolo-gy,1995,61:3943-3949.
[75] Kaye C,Milazzo J,Rozenfeld S et al.The development of simple sequence repeat markers for Magnaporthe grisea and their Integration into an established genetic linkage map[A].FungalGenetics and Biology,2003,40:207-214.
[76] Wet J,Burgess T,Slippers B et al.Multiple gene genealogies and Microsatellite markers reflect relationships between morphotypes of Sphaeropsis sapinea and distinguish a new species of Diplodi-a[A].Mycological Research,2003,107:557-566.
[77] Burgess T,Wingfiele MJ,Wingfield BW.Simple sequence repeat markers distinguish among mor-photypes of Sphaeropsis sapinea[A].Applied and Environmental Microbiology,2001,67:354-362.
[78] Hennequin C,Thieny A,Tichard GF et al.Microsatellite typing as a new tool for Identificationof Saccharomyces cerevisiae strains[A].Journal of Clinical Microbiology,2001,39:551-559.
[79] 张瑞颖.香菇菌株多相鉴定鉴别技术研究[D].中国农业大学:2004.
[80] Dunham SM,Dell TE,Molina R.Analysis of nrDNASequences and microsatellite allele frequencies reveals a Cryptic chanterelle species Cantharelluscus cascadensis sp.nov.from the American Pacific Northwest[A].Mycological Research,2003,107:1163-1177
[81] Hibbet DS,Fukumasa NY,Tsunoda A,Donoghue MJ.PHylogenetic diversity in shiitake inferred from nucear Ribosomal DNA sequences[A].Mycologia,1995,87:618-638.
[82] Guidot A,Lumini E,Debaud JC,Marmeisse R.The Nuclear Ribosomal DNA Intergenic Spacer as a Target Sequence To Study Intraspecific Diversity of the Ectomycorrhizal Basidiomycete Hobeloma cylindrosporum Directly on Pinus Root Systems[A].Apllied and Environmental Micr-obiology,1999,65:903-909.
[83] Baldridge GD,Dalton MW,Fallon AM.Is higherorder structure conserved in eukaryotic ribosomal DNA intergenic spacers?[A].Journal of Molecular Evolution,1992,35:514-523.
[84] Albee SR,Mueller GM,Kropp BR.Polymorphisms in the large intergenic spacer of the nuclear ribosomal repeat identify Laccaria proxima strains[A].Mycologia,1996,88:970-976.
[85] Ganley ARD,Barry S. Extraordinary Ribosomal Spacer Length Heterogeneity in a Neotyphodiu-m Endophyte Hybrid:Implications for Concerted Evolution[A].Genetics,1998,150:1625-1637.
[86] Szostak JW,Wu J.Unequal crossing over in the ribosomal DNA of Saccharomyces cemvisiae[A].Nature,1980,284:426-430.
[87] JacksonJ A,Fink GR.Gene conversion between duplicated genetic elements in yeast[A].Nature,1981,292:306-311.
[88] Mwenje E,Wingfield BD,Coetzee MP,Wingfield MJ. Molecular characterisation of Arrndlaria species from Zimbabwe[A].Mycological Research,2003,107:291-296
[89] Pramateftaki PV,Antoniou PP Types MA.The complete DNA sequence of the nuclear ribosom-al RNA gene complex of verticillium dahliae:intraspecific heterogeneity within the intergenic spacerregion[A].Fungal Genetics and Biology,2000,29:19-27.
[90] Sugita T,Kodama M,Saito M,Ito T,Kato Y,Tsuboi R,Nishikawa A.Sequence diversity of the intergenic spacer region of the rRNA gene of Malassezia globosa colonizing the skin of patients with atopic dermatitis and healthy individuals[A].Journal of Clinical Microbiology,2003,41:30 22-302.
[91] 张金霞,黄晨阳,张瑞颖等.中国栽培白灵侧耳的RAPD和IGS分析[J].菌物学报,2004,23(4):514-519.
[92] 黄晨阳,张金霞,郑素月等.刺芹侧耳(Pleurotuseyngii)rDNA的IGS2多样性分析[J].农业生物技术学报,2005,13(5):592-595.
[93] 邹容.天麻共生蜜环菌及同属真菌的基因系谱学及药用成分的研究[D].贵州大学:2005.
[94] Pei, MH,Whelan,MJ,Halford,NG. And Royle,DJ.Distinction between stem-and leaf-infecting forms of Melampsora rust on Salix viminalls using RAPD marks[A].Mycol.res.,1997,101(1):7-10.
[95] 余仲东,刘小勇,曹支敏.松杨栅锈菌的ITS序列和微卫星分析[J].中国农业科学,2006,39(6):1159-1165.
[96] Pino J,Frey P.Structue of Melampsora larici-populina populations on wild and cultivated popla-r[A].Eur.J.Plant Pathol., 1997,103(2):159-173.
[97] Lefevre F,Pichot C and Pionon J.Intra—and interspecific inheritance of some components of t-he resistance to leaf rust (Melampsora larici populina Kleb.)in poplars[A].Theor Appl.Genet,1994,88:501-507.
[98] Pinon J,Frey P.Miots, Villar M.The problem of durable resistance in the case of poplar rust(Melampsora larici-populina)[A].Durable Resistance Symposium,Wageningen(NL),2000.
[99] inon. J Reaction of main poplar clones to rust,particularly to Melampsora larici-populina[A].Revue Forestiere Francaise,1991,43(4):301-308.
[100] Picjot.Ch,Teissier Du Cros E. Susceptibility of P.deltoids Bartr to Melampsora larici-populinaand Melampsora alli-populina I.Quantitative analysis of a 6×6 factorial mating design[A].Siva-e Genetica,1993,42(4-5):179-187.
[101] Lefever F.Goue Mourier M C,Faivre Rampant P,etal.A single gene cluster controls in compati-bility and partial resistance to various Melampsora larici-populina races in hybrid poplars[A].Phytopathology,1998,88(2):156-163.
[102] Chiba Osamu,Study on the variation susceptibility and the nature of resistence of poplars to the leaf rust caused by Melampsora larici-populina Kleb.,Bullitin of the Government forest Experiment Station,No.166,Toyo,Japan.1994.
[103] Wilkinson,A.G.and Van Karraakyenoord,C.W.S.,Susceptibility classification of poplar clone to Melampsora rust in New Zealand[A].FAO/IPC Beograd,1975,19(4):942-952.
[104] 田呈明.落青杨叶锈病组织病理学和细胞学及病原菌遗传分化研究[D].杨陵:西北农林科技大学博士论文,2002:6
[105] 陈建珍,曹支敏,樊军锋.杨树叶锈病寄主抗性调查[J].西北林学院学报,2005,20(1):153-155.
[106] Siwecki,R.et al.Resistance mechanisms in interactions between poplars and rust.Resistance to d-isease and pests in forest trees[A].Wageningen,Centre for agricultural publishing and ducomentation,1982,130-142.
[107] Suzuki,K.et al.Studies on the susceptibility to poplar leaf rust influenced by the different nutr-ient condition 3 changes in free amino acid content[A].Journal of the Japanese Forestry Socie-t,1974,56(7):235-238.
[108] 胡 景 江 , 曹 支 敏 , 文 建 雷 . 落 叶 松 - 杨 栅 锈 菌 寄 主 抗 病 性 的 研 究 [J]. 林 业 科 学 研 究 , 2000,13(专):114-118.
[109] Szymanski M, et al. Nucleic Acids Res,2000,28:166-167.
[110] Einsele H, et al.J Clin Microbial,1997,35:1353-1360.
[111] 李振歧,商鸿生.小麦锈病及其防治[M].上海:上海科技出版社报,1989,211-229.
[112] 王廷华,景强.PCR理论与技术[M].北京:科学出版社,2005.
[113] 周俊宜.分子生物学基本技能和策略[M].北京:科学出版社,2003.
[114] 陈毓荃.生物化学实验方法和技术[M].北京:科学出版社,2002.
[115] 余 仲 东 , 高 爱 琴 , 曹 支 敏 . 锈 菌 夏 孢 子 DNA 的 微 量 快 速 提 取 方 法 [J]. 生 物 技 术 通讯,2005,16(1):48-50.
[116] 朱衡,瞿峰,朱立煌.利用氯化苄提取适于分子生物学分析的真菌DNA[J].真菌学报, 1994,13(1):34-40.
[117] 卢圣栋.现代分子生物学实验技术[M].北京:中国协和医科大学出版社,1999.
[118] 王国英.基因工程实验技术[M].北京:中国农业出版社,1997.
[119] 朱平.PCR 基因扩增实验操作手册[M].北京:中国科学技术出版社,1992.
[120] White T J,B runs TD,Lee SB,et al.Amp lif ication and direct sequencing of fungal ribosomal RNA genes for phylogenetics[A].Innis M A,Gelfand D H,Sninsky J J, et al PCR Protocols:A Guide to Methods and Applications[M ].New York: Academic Press,1990,315-321.
[121] Anderson J,Stasovski E.Molecular phylogeny of northern hemisphere species of Armillaria[A].Mycologia,1992,84(4):505~51.
[122] Timothy Y.James,Jean-Marc Moncalvo,Sean Li and Rytas Vilgalys.Polymorphism at the Ribos-omal DNA Spacers and Its Relation to Breeding Structure of the Widespread Mushroom Schizophyl-lum commune[J].Genetic,2001,157:149-161.
[123] Timothy Y.James and Rytas Vilgalys.Abundance and diversity of Schizophyllum commune spore clouds in the Caribbean detected by selective sampling[J].Molecular Ecology,2001,10:471-479.
[124] 李竟雄,宋同明著.植物细胞遗传学[M].北京:科学出版社,1997,1-51.
[125] 沈法富,刘风珍,于元杰.分子标记在植物育种中的应用[J].山东农业大学学报,28(1):83-91.
[126] 陆朝福,朱立煌.植物育种中分子标记辅助选择[J].生物工程进展,1995,15(4):11-17.
[127] 曹丽华.中国小麦条锈菌生理小种分子标记的研究[D].中国杨凌.2003.
[128] 金海国,姜成国,白红星等.分子遗传标记及其分析技术的研究进展[J].延边大学农学学报,2002,24(1):55—59.
[129] 梁宏,彭友良,张国珍等.腥黑粉菌属3中检疫性真菌rDNA-IGS区的扩增及其序列分析[J].植物病理学报,2006,36(5):407-412.
[130] 秦国夫,赵俊,田淑敏.北半球高卢蜜环菌的遗传多样性与分子鉴定[J].林业科学,2001,37(2):61 -69.
[131] 秦国夫,J.HANTULA.同宗配合蜜环菌与欧美异宗配合蜜环菌狭义种的分子系统学关系[J].菌物系统,2002,21(3):346-355.
[134] Vos P,Hogers R,Bleeker M et al.AFLP:a new technique for DNA fingerprinting[J].N uceic Acids
[135] Williams JGK,Hanafey MK,Rafalski JA.Genetic analysis using random amplified polymorphic DNA markers[J].Methodsin Enzymology,1993,218:704-740.Research,1995,23:4407-4414.
[136] Richards RI,Sutherland GR.Dynamic mutations:a new class of mutations causing human disea-se[J].Cell,1992,70:709-712.
[137] Wostemeyer J,Kreibich A.Repetitive DNA elements in fungi (Mycota):impact on genomic architecture and evolution[A].Current genetics,2002,41:189-198.
[138] Toth G,Gaspari Z,Jurka J.Mecrosatellites in diferent eukaryotic genomes:survey and analysis[J].Genome research,2000,10:967-981.
[139] 李传道.森林病害流行与治理[M].中国林业出版社,北京,1995:36-48.
[140] 方中达.植病研究法[M].北京:中国农业出版社,1998,361-362.
[141] 余仲东,张星耀,曹支敏.真菌核糖体基因间隔区研究概况[J].西北林学院学报,2000,15(2):107 -112.
[142] 余仲东,梅华全,曹支敏等.杨树锈病的PCR检测[J].西北林学院学报,2005,20(3):131-132.
[143] 林晓民,李振岐,王少先.真菌rDNA的特点及在外生菌根菌鉴定中的应用[J].西北农业学报,2005,14(2):120-125.
[144] 万兵,闫杰,季明春等.一种优化的PCR方法——降落PCR扩增目的基因[J].江苏临床医学,2002,6(2):127-129.
[145] 张贵星,袁保梅,许培荣等.一种高特异的改良降落PCR[J].生命科学研究,2002,6(4):314-317.
[146] 魏明,曹支敏,田呈明.中国落叶松杨栅锈菌4个流行小种的RAPD标记[J].2007,35(18):5366-5367, 5392.
[147] 金广峰,杨彦玲.环境因子对青杨锈病发生的影响[J].河北林业科技,2005,6:11.
[148] 郑淑霞,王占林.美洲黑杨×青杨杂交无性系抗锈病能力分析[J].青海农林科技,2004.42-43.
[149] 李瀚,曾汉清.青杨叶锈病流行规律及防治技术[J].陕西林业科技,1999,3:51-53.
[150] 李淑玲,侯建治,马浩.毛白杨不同无性系苗木抗锈性的初步研究[J].河南农业大学学报,1994,28(2):124-127.
[151] 祁承德,周卫芬,吴洪源等.落叶松杨锈病Melampsora larici-populina Kleb.发生规律及苗木损失量研究[J].青海农林科技,1995,2:27-30.