eIF-4E，VEGF和CD105在胃癌组织中的表达及其临床意义
摘要
目的:明确胃癌组织中eIF-4E,VEGF和CD105的表达情况及其临床意义。
方法:以53例接受了至少4个疗程的紫杉类、氟尿嘧啶类药物为主的联合化疗的胃癌病人为对象,采用免疫组织化学EnVision法,检测这53例胃癌组织中eIF-4E,VEGF和CD105的表达,同时检测10例癌旁正常组织中eIF-4E,VEGF和CD105的表达作对照。
结果:53例胃癌组织中eIF-4E,VEGF和CD105表达率分别是79.3%(42/53)、60.3%(32/53)和100%(53/53)。而在10例正常胃粘膜和癌旁组织中均无表达表达。eIF-4E,VEGF表达与年龄、性别、分化程度无关(P>0.05),与临床分期、淋巴结转移有关(P<0.05)。eIF-4E阳性组、阴性组的中位无病生存期(Disease free survive DFS)分别为8.7月、14.4月,中位总生存期(overall survive OS)分别为14.7月、20.4月,两组的差异均有统计学意义(P<0.05)。VEGF阳性组、阴性组中位DFS分别为8.3月、14.5月,中位OS值分别为17.0月、21.3月,两组的差异均有统计学意义(P<0.05)。通过Spearman秩相关检验发现eIF-4E的表达与VEGF的表达呈正相关(P<0.05),VEGF的表达与CD105的表达呈正相关(P<0.05)。
结论:eIF-4E,VEGF和CD105可能不仅与胃癌的发生有关,而且还可能与胃癌的侵袭潜能有关;eIF-4E,VEGF表达可能是胃癌患者预后的判断指标之一。
Objective: To study the expression of eIF-4E, VEGF and CD105 in gastric cancer tissues and investigate their relationships between their expressions and clinical pathological features.
Methods: The expressions of eIF-4E, VEGF and CD105 were detected in 53 tumor samples and from 53 gastric cancer patients treated with 5-fluorourcil and taxans-based adjuvant chemotherapy by immunohistochemical method and 10 normal stomach tissues were used as controls.
Results: The expressions of eIF-4E,VEGF and CD105 were 79.3%(42/53),60.3%(32/53)and 100%(53/53)in 53 gastric carcinoma. The eIF-4E and VEGF expression were closely related to the lymph node metastasis and TNM stage (P <0.05), but not to histological differentiation gender and ages.eIF-4E and VEGF were not expression in the normal tissues and paracarcinoma tissues. The disease free survival and the overall survival in the eIF-4E expression positive and negative gastric patients were 8.7mos, 14.7mos and 14.4mos, 20.4mos, respectively (P<0.05). The disease free survival and the overall survival were 8.3mos,17.0mos and 14.5mos,21.3mos in the VEGF expression positive and negative gastric patients, respectively(P <0.05).An positive correlation was found between eIF-4E and VEGF expression (P <0.05).
Conclusions: eIF-4E, VEGF and CD105 have different expression in gastric cancer tissues, while no expression in normal tissues and paracancer tissues. The expression of eIF-4E, VEGF and CD105 may not only has related with the development of gastric cancer, but also with the prognosis of gastric cancer.
方法:以53例接受了至少4个疗程的紫杉类、氟尿嘧啶类药物为主的联合化疗的胃癌病人为对象,采用免疫组织化学EnVision法,检测这53例胃癌组织中eIF-4E,VEGF和CD105的表达,同时检测10例癌旁正常组织中eIF-4E,VEGF和CD105的表达作对照。
结果:53例胃癌组织中eIF-4E,VEGF和CD105表达率分别是79.3%(42/53)、60.3%(32/53)和100%(53/53)。而在10例正常胃粘膜和癌旁组织中均无表达表达。eIF-4E,VEGF表达与年龄、性别、分化程度无关(P>0.05),与临床分期、淋巴结转移有关(P<0.05)。eIF-4E阳性组、阴性组的中位无病生存期(Disease free survive DFS)分别为8.7月、14.4月,中位总生存期(overall survive OS)分别为14.7月、20.4月,两组的差异均有统计学意义(P<0.05)。VEGF阳性组、阴性组中位DFS分别为8.3月、14.5月,中位OS值分别为17.0月、21.3月,两组的差异均有统计学意义(P<0.05)。通过Spearman秩相关检验发现eIF-4E的表达与VEGF的表达呈正相关(P<0.05),VEGF的表达与CD105的表达呈正相关(P<0.05)。
结论:eIF-4E,VEGF和CD105可能不仅与胃癌的发生有关,而且还可能与胃癌的侵袭潜能有关;eIF-4E,VEGF表达可能是胃癌患者预后的判断指标之一。
Objective: To study the expression of eIF-4E, VEGF and CD105 in gastric cancer tissues and investigate their relationships between their expressions and clinical pathological features.
Methods: The expressions of eIF-4E, VEGF and CD105 were detected in 53 tumor samples and from 53 gastric cancer patients treated with 5-fluorourcil and taxans-based adjuvant chemotherapy by immunohistochemical method and 10 normal stomach tissues were used as controls.
Results: The expressions of eIF-4E,VEGF and CD105 were 79.3%(42/53),60.3%(32/53)and 100%(53/53)in 53 gastric carcinoma. The eIF-4E and VEGF expression were closely related to the lymph node metastasis and TNM stage (P <0.05), but not to histological differentiation gender and ages.eIF-4E and VEGF were not expression in the normal tissues and paracarcinoma tissues. The disease free survival and the overall survival in the eIF-4E expression positive and negative gastric patients were 8.7mos, 14.7mos and 14.4mos, 20.4mos, respectively (P<0.05). The disease free survival and the overall survival were 8.3mos,17.0mos and 14.5mos,21.3mos in the VEGF expression positive and negative gastric patients, respectively(P <0.05).An positive correlation was found between eIF-4E and VEGF expression (P <0.05).
Conclusions: eIF-4E, VEGF and CD105 have different expression in gastric cancer tissues, while no expression in normal tissues and paracancer tissues. The expression of eIF-4E, VEGF and CD105 may not only has related with the development of gastric cancer, but also with the prognosis of gastric cancer.
引文
[1] De Benedetti A,GraftJR.eIF-4E expression and its role in malignancies and metastases[J].Oncogene,2004,23(18):3189-3199.
[2] Rosenwald IB.The role of translation in neoplastic transformation from a pathologist′s point of view [J]. Oncogene,2004,23(18): 3230- 3247.
[3] Norton KS, McCulusky D, Sen S,et al. TLKIB is elevated with eIF-4E overexpression in breast cancer [J].J Surg Res, 2004,116 (1):98-103.
[4] McClusky DR,Chu Q, Yu H, et al. A prospective trial on initiation factor 4E (eIF-4E)overexpression and cancer recurrence in node-positive breast cancer [J].Annals of Surgery 2005,242(4):584-592.
[5] Byrnes K, White S, Chu Q, et al. High eIF-4E, VEGF and microvessel density in stage I to III breast cancer [J]. Annals of Surgery, 2006,243(5): 684-692.
[6] Chen CN,Hsieh FJ,Cheng YM,et a1.Expression of eukaryotic initiation factor 4E in gastric adenocarcinoma and its association with clinical outcome[J].J surg Oncol,2004,86(1):22—27.
[7] Takahashi R,Tanaka S,Kitadai Y,et a1.Expression of vascular endotheothelial growth factor and angiogenesis in gastrointestinal stromal tumor of the stomach[J]. Oncology,2003,64(3)266-274.
[8] Mamane Y,Petroulakis E, Rong L, et al.eIF4E from translation totransformation [J].Oncogene,2004,23(18):3172-3179.
[9] De Benedetti A, Graft JR.eIF-4E expression and its role in malignancies and metastases[J].Oncogene,2004,23(18):3189—3199.
[10] Yang SX, Hewitt SM,Steinberg SM, et al. Expression levels of eIF4E, VEGF, and cyclin D1, and correlation of eIF-4E with VEGF and cyclin D1 in multi-tumor tissue microarray[J].OncolRep, 2007,17(2):281-287.
[11] Menezes C,De Benedetti A,Abreo F, et al. A survey of the proto-oncegene/translation factor eIF-4E in head and neck squamous cell cancer[J]. Otolaryngol Head Neck Surg,1996,l15(3):72—73.
[12] Moldovan NI. Angiogenesis, terrible of vascular biology is coming of age [J].J Cell Mol Med,2005,9(4):775-776.
[13] Tokunaga T, Oshika Y, Abe Y,et al. Vascular endothelial Growth factor (VEGF) mRNA isoform expression pattern is correlated with live metastasis and poor prognosis in colon cancer [J].Br J Cancer,1998,77(6):998-1002.
[14] Kumar P,Wang JM, Bernabeu C,et al.CD105 and angiogenesis [J].J Pathol,1996,178(4):363-366.
[15] Fox SH, Whalen GF, Sander MM, et al. Angiogenesis in normal tissue adjacent to colon cancer [J].J surg Oncol.1998,69 (4):230-234.
[16]于建宪,陈桦,张七一等.胃癌组织中TGF-β1表达与微血管密度的关系[J].齐鲁医药杂志,2004,19(1):33-35.
[17] Sharma S, Sharma MC, Sarkar C. Morphology of angiogenesis in human cancer: a conceptual overview, histoprognostic perspective and significance of neoangiogenesis [J]. Histopathology ,2005,46(5):481-489.
[1] De Benedetti A, GraftJR. eIF-4E expression and its role in malignancies and metastases[J].Oncogene,2004,23(18):3189 -3199.
[2] Strudwick S ,Borden KL. The emerging role of translation factor eIF-4E in the nucleus [J] Differentiation,2002,70(1):10—22.
[3] Kapp LD, Lorsch JR.The molecular mechanics of eukaryotic translation [J]. Annu Rev Biochem.2004,73(2):657-704.
[4] Mamane Y, Pet roulakis E , Rong L,et al.eIF-4E from translation to transformation [J].Oncogene,2004,23(18):3172-3179.
[5] De Benedetti A,GraftJR.eIF-4E expression and its role in malignancies and metastases[J].Oncogene,2004,23(18):3189 -3199.
[6] Gingras AC,Kennedy SG,Oleary MA, et al.eIF-4EBP1a repressor of mRNA translation is phosphorylated and inactivated by the AKT(PKB)signaling pathway[J].Genes Dev,1998,12(4):502-513.
[7] Yang D,Brunn GJ,Lawrence J, et al. Mutational analysis of sites in the translational rugulator PHAS-1 that are selectively phosphorylated by mTOR[J].Febs lett,1999, 453(3):387-390.
[8] Zhang Y, Li Y, Yang DQ. Phosphorylation of eIF-4E positively regulates formation of the eIF-4F translation initiation complex following DNA damage[J].Biochem Biophys Res Commun, 2008,367(1):54-59.
[9] Rosenwald IB.The role of translation in neoplastic transformation from apathologist′s point of view [J]. Oncogene,2004,23(18):3230-3247.
[10] Rinker SCW,Graft JR,Benedetti D,et a1.Decreasing the level of translation initiation factor 4E with antisense RNA caused reveral of rasmedliated transformation and tumorigenesis of cloned rat embryo fibroblasts[J].hit J Cancer,1993,55(5):841—847.
[11] Yang SX, Hewitt SM,Steinberg SM, et al. Expression levels of eIF-4E, VEGF, and cyclin D1, and correlation of eIF4-E with VEGF and cyclin D1 in multi-tumor tissue microarray[J].Oncol Report,2007,17(2):281-287.
[12] Menezes C,De Benedetti A,Abreo F,et al. A survey of the proto-oncegene/ translation factor eIF-4E in head and neck squamous cell cancer[J]. Otolaryngol Head Neck Surg, 1996, l15(3):72—73.
[13] Haydon MS,Googe JD,Sorrells DS,et a1.Progression of eIF-4E gene amplification and overexpression in benign and malignant tumors of the head and neck[J]. Cancer,2000,88(12):2803—2810.
[14] SorreUs D,Ghali G,Benedetti A,et a1.Competitive PCR to detect eIF-4E gene amplification in head and neck cancer [J] Head and neck,1999,21(1):60-65.
[15] Chandy B, Abreo F,Nassar R,et al.Expression of the proto-oncogene eIF-4E in inflammation of the oral cavity[J]. Otolaryngol Head Neck Surg,2002,126(3):290-295.
[16] Nathan CA,Amirghahri N,Rice C,et al.Molecular analysis of surgical margins in head and neck squamous cell cacinoma patients[J]. Laryngoscope,2002, 112(12): 2129-2140.
[17]罗文政,张剑,江红群等.现代肿瘤学[J].2005,13(6):753-755.
[18] Kerekatte V,Smiley k,Hu B,et a1.The proto-oncegene/translation factor eIF-4E:a survey of its explanstion breast carcinomas[J].Int J Cancer,1995,64(1):27-31.
[19] Norton K S,Iculusky D,Sen S.TLKIB is elevated with eIF-4E overexpression in breast cancer[J].J Surg Res,2004,116 (1):98-103.
[20] Derek R,McClusky,Quyen C,et al.A Prospective Trial on Initiation Factor 4E (eIF-4E)Overexpression and Cancer Recurrence in Node-PositiveBreast Cancer[J]. Annals of Surgery, 2005,242(4):584-592.
[21] Kerry B,Stephen W,Quyen C,et al. High eIF4E, VEGF, and Microvessel Densityin Stage I to III Breast Cancer[J]. Annals of Surgery,2006,243(5):684-692.
[22] Zhou S, Wang G P, Liu C,et al. Eukaryotic Initiation Factor 4E (eIF-4E) and angiogenesis prognostic markers for breast cancer[J].BMC Cancer,2006,231(6):21-33.
[23] Zivar S , Farhad M. Expression of the eukaryotic translation initiation factor4E (eIF-4E) and 4E-BP1 in esophageal cancer [J].Clinical Biochemistry,2006,39(12):404–409.
[24]邱刚,陈奎生,权翼龙.真核始动因子4E表达与食管癌转移关系的研究[J].肿瘤基础和临床,2007,20(3):206-207.
[25] Chen CN,Ltsieh FJ,Cheng YM,et a1.Expression of eukaryotic initiation factor 4E in gastric adenocarcinoma and its association with clinical outcome[J].J surg Oncol,2004,86(1):22—27.
[26]熊碧芳,方向明,胡继军.91例胃癌患者真核细胞起始因子与血管内皮生长因子的表达及其临床意义[J].临床内科杂志,2005,22 (11):743-745.
[27]王坤,耿敬姝.胃癌中eIF-4E、cyclinD1及P27的表达及其意义[J].齐齐哈尔医学院报,2007,28(3):277-299.
[28]熊碧芳,方向明,胡继军等.胃癌eIF4E和cyclin D1表达及其临床病理意义[J].肿瘤防治研究,2006,33(2):97-99.
[29] Martin N,PerezV,Redondo C,et al. eIF-4E binding protein 1 expression is inversely correlated to the progression of gastrointestinal cancers [J].The International Journal of Biochemistry & Cell Biology ,2000,32(6):633-642.
[30] Hans J, Elba A,Turbat H, et al.Expression of the Translation Initiation Factor eIF4E in the Polyp-Cancer Sequence in the Colon[J]. Cancer Epidemiology Biomarkers & Prevention,2001,10(6):663–666.
[31] Rosenwald IB,Chen JJ,Wang S,et al.Upregulation of protein synthesis initiation factor eIF-4E is an early event during colon carcinogenesis [J]. Oncogene,1999, 18(15): 2507-2517.
[33] Seki N,Tckasu T,Mandai K,et a1.Expression of eukaryotic initiation factor 4E in atypical adenomatous hyperplasia and adenocarecinoma of the human peripheral lung[J].Clin Cancer Res,2002,8(10):3046-3053.
[33] Zhao Y, Liu W, Zhou S, et al. Relationship between eukaryotic translation initiation factor 4E and malignant angiogenesis in non-Hodgkin lymphoma[J]. Huazhong Univ Sci Technolog Med Sci, 2005,25(6):636-654.
[34] Jeremy R,Bruce W,Thomas M.Therapeutic suppression of translation initiation factor eIF-4E expression reduces tumor growth without toxicity[J].the Journal of Clinical Investigation,2007,117(9):2638-2648.
[35] Byrnes K, Li BD, Holm N,et al.A novel suicide gene therapy targeting the overexpression of eukaryotic initiation factor 4E improves survival in a rat peritoneal carcinomatosis model[J].surgery,2007,142(2):270-275.
[36] Lee JW, Choi JJ, Lee KM, et al.eIF-4E expression is associated with histopathologic grades in cervical neoplasia[J]. Hum Pathol,2005 36(11):1197-1203.
[2] Rosenwald IB.The role of translation in neoplastic transformation from a pathologist′s point of view [J]. Oncogene,2004,23(18): 3230- 3247.
[3] Norton KS, McCulusky D, Sen S,et al. TLKIB is elevated with eIF-4E overexpression in breast cancer [J].J Surg Res, 2004,116 (1):98-103.
[4] McClusky DR,Chu Q, Yu H, et al. A prospective trial on initiation factor 4E (eIF-4E)overexpression and cancer recurrence in node-positive breast cancer [J].Annals of Surgery 2005,242(4):584-592.
[5] Byrnes K, White S, Chu Q, et al. High eIF-4E, VEGF and microvessel density in stage I to III breast cancer [J]. Annals of Surgery, 2006,243(5): 684-692.
[6] Chen CN,Hsieh FJ,Cheng YM,et a1.Expression of eukaryotic initiation factor 4E in gastric adenocarcinoma and its association with clinical outcome[J].J surg Oncol,2004,86(1):22—27.
[7] Takahashi R,Tanaka S,Kitadai Y,et a1.Expression of vascular endotheothelial growth factor and angiogenesis in gastrointestinal stromal tumor of the stomach[J]. Oncology,2003,64(3)266-274.
[8] Mamane Y,Petroulakis E, Rong L, et al.eIF4E from translation totransformation [J].Oncogene,2004,23(18):3172-3179.
[9] De Benedetti A, Graft JR.eIF-4E expression and its role in malignancies and metastases[J].Oncogene,2004,23(18):3189—3199.
[10] Yang SX, Hewitt SM,Steinberg SM, et al. Expression levels of eIF4E, VEGF, and cyclin D1, and correlation of eIF-4E with VEGF and cyclin D1 in multi-tumor tissue microarray[J].OncolRep, 2007,17(2):281-287.
[11] Menezes C,De Benedetti A,Abreo F, et al. A survey of the proto-oncegene/translation factor eIF-4E in head and neck squamous cell cancer[J]. Otolaryngol Head Neck Surg,1996,l15(3):72—73.
[12] Moldovan NI. Angiogenesis, terrible of vascular biology is coming of age [J].J Cell Mol Med,2005,9(4):775-776.
[13] Tokunaga T, Oshika Y, Abe Y,et al. Vascular endothelial Growth factor (VEGF) mRNA isoform expression pattern is correlated with live metastasis and poor prognosis in colon cancer [J].Br J Cancer,1998,77(6):998-1002.
[14] Kumar P,Wang JM, Bernabeu C,et al.CD105 and angiogenesis [J].J Pathol,1996,178(4):363-366.
[15] Fox SH, Whalen GF, Sander MM, et al. Angiogenesis in normal tissue adjacent to colon cancer [J].J surg Oncol.1998,69 (4):230-234.
[16]于建宪,陈桦,张七一等.胃癌组织中TGF-β1表达与微血管密度的关系[J].齐鲁医药杂志,2004,19(1):33-35.
[17] Sharma S, Sharma MC, Sarkar C. Morphology of angiogenesis in human cancer: a conceptual overview, histoprognostic perspective and significance of neoangiogenesis [J]. Histopathology ,2005,46(5):481-489.
[1] De Benedetti A, GraftJR. eIF-4E expression and its role in malignancies and metastases[J].Oncogene,2004,23(18):3189 -3199.
[2] Strudwick S ,Borden KL. The emerging role of translation factor eIF-4E in the nucleus [J] Differentiation,2002,70(1):10—22.
[3] Kapp LD, Lorsch JR.The molecular mechanics of eukaryotic translation [J]. Annu Rev Biochem.2004,73(2):657-704.
[4] Mamane Y, Pet roulakis E , Rong L,et al.eIF-4E from translation to transformation [J].Oncogene,2004,23(18):3172-3179.
[5] De Benedetti A,GraftJR.eIF-4E expression and its role in malignancies and metastases[J].Oncogene,2004,23(18):3189 -3199.
[6] Gingras AC,Kennedy SG,Oleary MA, et al.eIF-4EBP1a repressor of mRNA translation is phosphorylated and inactivated by the AKT(PKB)signaling pathway[J].Genes Dev,1998,12(4):502-513.
[7] Yang D,Brunn GJ,Lawrence J, et al. Mutational analysis of sites in the translational rugulator PHAS-1 that are selectively phosphorylated by mTOR[J].Febs lett,1999, 453(3):387-390.
[8] Zhang Y, Li Y, Yang DQ. Phosphorylation of eIF-4E positively regulates formation of the eIF-4F translation initiation complex following DNA damage[J].Biochem Biophys Res Commun, 2008,367(1):54-59.
[9] Rosenwald IB.The role of translation in neoplastic transformation from apathologist′s point of view [J]. Oncogene,2004,23(18):3230-3247.
[10] Rinker SCW,Graft JR,Benedetti D,et a1.Decreasing the level of translation initiation factor 4E with antisense RNA caused reveral of rasmedliated transformation and tumorigenesis of cloned rat embryo fibroblasts[J].hit J Cancer,1993,55(5):841—847.
[11] Yang SX, Hewitt SM,Steinberg SM, et al. Expression levels of eIF-4E, VEGF, and cyclin D1, and correlation of eIF4-E with VEGF and cyclin D1 in multi-tumor tissue microarray[J].Oncol Report,2007,17(2):281-287.
[12] Menezes C,De Benedetti A,Abreo F,et al. A survey of the proto-oncegene/ translation factor eIF-4E in head and neck squamous cell cancer[J]. Otolaryngol Head Neck Surg, 1996, l15(3):72—73.
[13] Haydon MS,Googe JD,Sorrells DS,et a1.Progression of eIF-4E gene amplification and overexpression in benign and malignant tumors of the head and neck[J]. Cancer,2000,88(12):2803—2810.
[14] SorreUs D,Ghali G,Benedetti A,et a1.Competitive PCR to detect eIF-4E gene amplification in head and neck cancer [J] Head and neck,1999,21(1):60-65.
[15] Chandy B, Abreo F,Nassar R,et al.Expression of the proto-oncogene eIF-4E in inflammation of the oral cavity[J]. Otolaryngol Head Neck Surg,2002,126(3):290-295.
[16] Nathan CA,Amirghahri N,Rice C,et al.Molecular analysis of surgical margins in head and neck squamous cell cacinoma patients[J]. Laryngoscope,2002, 112(12): 2129-2140.
[17]罗文政,张剑,江红群等.现代肿瘤学[J].2005,13(6):753-755.
[18] Kerekatte V,Smiley k,Hu B,et a1.The proto-oncegene/translation factor eIF-4E:a survey of its explanstion breast carcinomas[J].Int J Cancer,1995,64(1):27-31.
[19] Norton K S,Iculusky D,Sen S.TLKIB is elevated with eIF-4E overexpression in breast cancer[J].J Surg Res,2004,116 (1):98-103.
[20] Derek R,McClusky,Quyen C,et al.A Prospective Trial on Initiation Factor 4E (eIF-4E)Overexpression and Cancer Recurrence in Node-PositiveBreast Cancer[J]. Annals of Surgery, 2005,242(4):584-592.
[21] Kerry B,Stephen W,Quyen C,et al. High eIF4E, VEGF, and Microvessel Densityin Stage I to III Breast Cancer[J]. Annals of Surgery,2006,243(5):684-692.
[22] Zhou S, Wang G P, Liu C,et al. Eukaryotic Initiation Factor 4E (eIF-4E) and angiogenesis prognostic markers for breast cancer[J].BMC Cancer,2006,231(6):21-33.
[23] Zivar S , Farhad M. Expression of the eukaryotic translation initiation factor4E (eIF-4E) and 4E-BP1 in esophageal cancer [J].Clinical Biochemistry,2006,39(12):404–409.
[24]邱刚,陈奎生,权翼龙.真核始动因子4E表达与食管癌转移关系的研究[J].肿瘤基础和临床,2007,20(3):206-207.
[25] Chen CN,Ltsieh FJ,Cheng YM,et a1.Expression of eukaryotic initiation factor 4E in gastric adenocarcinoma and its association with clinical outcome[J].J surg Oncol,2004,86(1):22—27.
[26]熊碧芳,方向明,胡继军.91例胃癌患者真核细胞起始因子与血管内皮生长因子的表达及其临床意义[J].临床内科杂志,2005,22 (11):743-745.
[27]王坤,耿敬姝.胃癌中eIF-4E、cyclinD1及P27的表达及其意义[J].齐齐哈尔医学院报,2007,28(3):277-299.
[28]熊碧芳,方向明,胡继军等.胃癌eIF4E和cyclin D1表达及其临床病理意义[J].肿瘤防治研究,2006,33(2):97-99.
[29] Martin N,PerezV,Redondo C,et al. eIF-4E binding protein 1 expression is inversely correlated to the progression of gastrointestinal cancers [J].The International Journal of Biochemistry & Cell Biology ,2000,32(6):633-642.
[30] Hans J, Elba A,Turbat H, et al.Expression of the Translation Initiation Factor eIF4E in the Polyp-Cancer Sequence in the Colon[J]. Cancer Epidemiology Biomarkers & Prevention,2001,10(6):663–666.
[31] Rosenwald IB,Chen JJ,Wang S,et al.Upregulation of protein synthesis initiation factor eIF-4E is an early event during colon carcinogenesis [J]. Oncogene,1999, 18(15): 2507-2517.
[33] Seki N,Tckasu T,Mandai K,et a1.Expression of eukaryotic initiation factor 4E in atypical adenomatous hyperplasia and adenocarecinoma of the human peripheral lung[J].Clin Cancer Res,2002,8(10):3046-3053.
[33] Zhao Y, Liu W, Zhou S, et al. Relationship between eukaryotic translation initiation factor 4E and malignant angiogenesis in non-Hodgkin lymphoma[J]. Huazhong Univ Sci Technolog Med Sci, 2005,25(6):636-654.
[34] Jeremy R,Bruce W,Thomas M.Therapeutic suppression of translation initiation factor eIF-4E expression reduces tumor growth without toxicity[J].the Journal of Clinical Investigation,2007,117(9):2638-2648.
[35] Byrnes K, Li BD, Holm N,et al.A novel suicide gene therapy targeting the overexpression of eukaryotic initiation factor 4E improves survival in a rat peritoneal carcinomatosis model[J].surgery,2007,142(2):270-275.
[36] Lee JW, Choi JJ, Lee KM, et al.eIF-4E expression is associated with histopathologic grades in cervical neoplasia[J]. Hum Pathol,2005 36(11):1197-1203.