日粮蛋白质水平对仔猪脂肪酸合成关键酶及其代谢产物的影响
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摘要
本试验通过研究饲粮蛋白水平对断奶仔猪生产性能、血清脂质含量、脂肪酸合成关键酶含量、基因表达及其代谢产物的影响,旨在从代谢水平上证明蛋白质水平对脂肪酸合成的调控作用。试验采用单因素试验设计,选取24±1日龄断奶的DLY仔猪24头,按照体重一致,公母各半的原则随机分配到3个处理组中,每个处理4个重复,每个重复两头猪。各处理日粮能量一致,蛋白水平分别为23%(高蛋白)、20%(中蛋白)和17%(低蛋白)。试验日粮主要参照NRC(1998)5-10kg仔猪营养需要进行配制,试验期28天。试验结果表明:
(1)高蛋白日粮显著提高仔猪生产性能,中低蛋白日粮则无明显差异。
(2)高蛋白日粮趋向于降低仔猪血清甘油三酯,总胆固醇,极低密度脂蛋白-胆固醇水平,中低蛋白日粮对血脂水平的影响差异不显著。
(3)高蛋白日粮极显著降低仔猪肝脏中FAS的活力(p＜0.01),极显著降低肝脏和脂肪组织中ACC蛋白水平及其mRNA的相对表达量(p＜0.01)。
(4)高蛋白日粮有降低肝脏丙二酰辅酶A趋势,与FAS相比ACC对脂肪酸合成的限速能力较弱。
本实验结果证明,高蛋白日粮对脂质代谢的调控作用,主要是通过抑制ACC的基因表达和降低FAS的酶活,降低脂肪酸合成,减少体脂沉积,提高生产性能;与FAS相比ACC对脂肪酸合成的限速能力较弱。肝脏脂质代谢的丙二酰辅酶A途径对日粮蛋白水平引起的脂质代谢的影响较小,不具备调控脂质代谢的作用。
To explore the role of diet protein level on regulating lipid metabolism,the current trials were designed to determine the effects of diet protein level on performance,blood lipid,the relative enzymes' activities and mRNA expression and their production.A one-factor experiment was designed.24 DLY weanling pigs averaged 6.25kg were randomly allocated to 3 treatments with 4 replicates,each replicate 2 pigs.The same DE of the experiment diets were disigned and protein level were 23%(high level),20%(middle level ),17%(low level).The experiment diets were prepared according NRC(1998) nutrient requirements of 5-10kg pigs.he experiment lasted 4 weeks.The results showed that:
(1):Diet of high protein level can improved performance,there were no different between middle protein level and low protein level.
(2):Diet of high protein level tend to decrease serum TG,TC and VLDL-C,there were no different between middle protein level and low protein level.
(3):Diet of high protein level can decrease FAS activity extremely significantly in liver(p＜0.01);decrease ACC protein level and its mRNA correspondence quantity extremely significantly in liver and adipose tissues.
(4):Diet of high protein level tend to decrease Malonyl-CoA in liver tissue,and compared with FAS,ACC was poor in limiting synthesis of fatty acid.
It is concluded that:high protein regulate lipid metabolism mainly by inhibit ACC gene expression and decrease the activity of FAS,so that inhibit the fatty acid synthesis of weanling pigs and decrease lipidosis,improve their performance; Compared with FAS,ACC is poor of controlling fatty acid synthesis;There is no effect of M-CoA on lipometabolism caused by protein level.
(1)高蛋白日粮显著提高仔猪生产性能,中低蛋白日粮则无明显差异。
(2)高蛋白日粮趋向于降低仔猪血清甘油三酯,总胆固醇,极低密度脂蛋白-胆固醇水平,中低蛋白日粮对血脂水平的影响差异不显著。
(3)高蛋白日粮极显著降低仔猪肝脏中FAS的活力(p＜0.01),极显著降低肝脏和脂肪组织中ACC蛋白水平及其mRNA的相对表达量(p＜0.01)。
(4)高蛋白日粮有降低肝脏丙二酰辅酶A趋势,与FAS相比ACC对脂肪酸合成的限速能力较弱。
本实验结果证明,高蛋白日粮对脂质代谢的调控作用,主要是通过抑制ACC的基因表达和降低FAS的酶活,降低脂肪酸合成,减少体脂沉积,提高生产性能;与FAS相比ACC对脂肪酸合成的限速能力较弱。肝脏脂质代谢的丙二酰辅酶A途径对日粮蛋白水平引起的脂质代谢的影响较小,不具备调控脂质代谢的作用。
To explore the role of diet protein level on regulating lipid metabolism,the current trials were designed to determine the effects of diet protein level on performance,blood lipid,the relative enzymes' activities and mRNA expression and their production.A one-factor experiment was designed.24 DLY weanling pigs averaged 6.25kg were randomly allocated to 3 treatments with 4 replicates,each replicate 2 pigs.The same DE of the experiment diets were disigned and protein level were 23%(high level),20%(middle level ),17%(low level).The experiment diets were prepared according NRC(1998) nutrient requirements of 5-10kg pigs.he experiment lasted 4 weeks.The results showed that:
(1):Diet of high protein level can improved performance,there were no different between middle protein level and low protein level.
(2):Diet of high protein level tend to decrease serum TG,TC and VLDL-C,there were no different between middle protein level and low protein level.
(3):Diet of high protein level can decrease FAS activity extremely significantly in liver(p＜0.01);decrease ACC protein level and its mRNA correspondence quantity extremely significantly in liver and adipose tissues.
(4):Diet of high protein level tend to decrease Malonyl-CoA in liver tissue,and compared with FAS,ACC was poor in limiting synthesis of fatty acid.
It is concluded that:high protein regulate lipid metabolism mainly by inhibit ACC gene expression and decrease the activity of FAS,so that inhibit the fatty acid synthesis of weanling pigs and decrease lipidosis,improve their performance; Compared with FAS,ACC is poor of controlling fatty acid synthesis;There is no effect of M-CoA on lipometabolism caused by protein level.
引文
[1]李亮,程彦伟.乙酰辅酶A羧化酶在治疗肥胖中的潜在作用[J],生命的化学,2007年27卷2期:180-182.
[2]Moibi J A,Ekpe E D,and Christopherson R J.Acetyl-CoA carboxylase and fatty acid synthase activity and immunodetectable protein in adipose tissues of ruminants:Effect of temperature and feeding level.J Anim Sci,2000,78:2383-2392
[3]季海峰,张沅.猪脂肪代谢的研究进展[J].中国畜牧杂志,1993,29(3):59-61.
[4]Iritani N,Hosomi H,Fukuda H,Tada K,et al.Soybean protein suppresses hepatic lipogenic enzyme gene expression in Wistar fatty rats[J].J.Nutr,1996,126,380-388.
[5]Iritani N,Suga A,Fukuda H,Katsurada A,et al.Effects of dietary casein and soybean protein on triacylglycerol turnover in rat liver[J]J.Nutr.Sci.Vitaminol,1988,34:309-315.
[6]Shinjo S,Asato L,Arakaki S,Kina T,et al.Comparative effect of casein and soybean protein isolate on body fat accumulation in adult rats[J].J.Nutr.Sci.Vitaminol.,1992,38:247-253.
[7]Aoyama Y,Wada M.Supplementation of orotic acid to the casein,but not to egg protein,soy protein,or wheat gluten diets,increases serum ornithine carbamoyltransferase activity[J].J.Nutr.Biochem.,2000,11:306-310.
[8]Aoyama T,Fukui K,Takamatsu K,Hashimoto Y,et al.Soy protein isolate and its hydrolysate reduce body fat of dietary obese rats and genetically obese mice(Yellow KK)[J].Nutrition,2000,16:349-354.
[9]Ide T,Murata M,Sunada Y.Soybean protein dependent changes in triacylglycerol synthesis and concentration of diacylglycerol in the liver microsomes of fasted-refed rats[J].Ann.Nutr.Metab,1992,36:87-96.
[10]Lapre J A,West C E,Loveti M R,Sirtori C R,et al.Dietary animal proteins and cholesterol metabolism in rats[J].Int.J.Vitam.Nutr.Res,1989,59:93-100.
[11]Iritani N,Sugimoto T,Fukuda H,Komiya M et al.Dietary soybean protein increases insulin receptor gene expression in Wistar fatty rats when dietary polyunsaturated fatty acid level is low[J].J Nutr,1997,127:1077-1083.
[12]Berdanier C D,Kras K M,Wickwire K Hall D G.Whole-egg diet delays the age-related impaired glucose tolerance of BHE/Cdb rats[J].Proc.Soc.Exp.Biol. Med, 1998, 219:28-36(1998).
[13]Mikkelsen P B,Toubro S,Astrup A. Effect of fat reduced diets on 24-h energy expenditure: comparisons between animal protein, vegetable protein, and carbohydrate[J].Am. J. Clin. Nutr,2000,72:1135-1141.
[14]Gannon M C,Nuttall F Q,Neil B J,Westphal S A.The insulin and glucose responses to meals of glucose plus various proteins in type II diabetic subjects[J].Metabloism, 1988,37:1080-1088
[15]Iritani N,Nagashima K,Fukuda H,Katsurada A et al.Effects of dietary proteins on lipogenic enzymes in rat liver[J].J. Nutr, 1986,116:190-197.
[16]Aoki H,Kimura K,Igarashi K,Takenaka A.Soy protein suppresses gene expression of acetyl-coA carboxylase alpha from promoter PI in rat liver[J]. Biosci. Biotechnol. Biochem, 2006, 70(4): 843-849.
[17]Webster A J F.Energy partitioning, tissue growth and appetite control[J].Proc. Nutr. Soc, 1993,52: 69-76.
[18]White B D,He B,Dean R G,Martin R J.Low protein diets increase neuropeptide Y gene expression in the basomedial hypothalamus of rats [J]. J.Nutr,1994, 124:1152 -1160.
[19]Du F Y,Higginbotham D A,White B D.Food intake,energy balance and serum leptin concentrations in rats fed low-protein diets[J].J.Nutr,2000,130:514-521.
[20]Adeola O, Young L G. Dietary protein-induced changes in porcine muscle respiration, protein synthesis and adipose tissue metabolism [J]. J. Anim. Sci, 1989,67:664-673.
[21]Wood J D,S N Brown,G R Nute, F M Whittington,et al.Effects of breed, feed level and conditioning time on the tenderness of pork[J],Meat Sci, 1996, 44: 105-112.
[22] Candek P M,L Lefaucheur,B,Zlender,M Bonneau.Effect of age and/or weight at slaughter on longissimus dorsi muscle: biochemical traits and sensory quality in pigs[J].Meat Sci,1998,48:287-300.
[23] Karlsson A,A C Enfa¨lt, B Essen-Gustavsson, K Lundstro¨m,et al.Muscle histochemical and biochemical properties in relation to meat quality during selection for increased lean tissue growth rate in pigs[J]. J. Anim. Sci, 1993,71: 930-938.
[24]Gondret F,Lebret B. Feeding intensity and dietary protein level affect adipocyte cellularity and lipogenic capacity of muscle homogenates in growing pigs, without modification of the expression of sterol regulatory element binding protein[J].J Anim.Sci,2002.80:3184-3193.
[25]Rosebrough R W,Steele N C.Dietary crude protein levels and the effect of isoproterenol on in vitro lipogenesis in the chicken[J].J.Nutr,1990,120:1684-1691.
[26]Mldner AM,Clarke SD.Porcine fatty acid synthase:Cloning of a complementary DNA,tissue distribution of its mRNA and suppression of expression by somatomin and dietary protein[J].J.Nutr,1991,121:900-907.
[27]Katsurada A,Ritanin,Fukuda H,et al.Effects of nutrients and homones on transcriptional and post-transcriptional regulation of acetyl-CoA carboxylase in rat liver[J].Eur Biochem,190(2):435-441.
[28]McGarry J D,Brown N F.The Mitochondrial Carnitine Palmitoyltransferase System-From Concept to Molecular Analysis[J].Eur J Biochem,1997,244(1):1-14
[29]Clifford D L,Folmes G D,Lopaschuk.Role of malonyl-CoA in heart disease and the hypothalamic control of obesity[J].Cardiovascular Research,2007,73:278-287
[30]White JD.Low protein diets increase neuropetide Y gene expression in the basomedial hypothalamus of rats[J].J Nutr,1994,124:1152-1160.
[31]Abu-Elheiga L,Matzuk M M,Kordari P,Oh W,et al.Mutant mice lacking acetyl-CoA carboxylase Ⅰ are embryonically lethal[J].Proc Natl Acad Sci USA,2005,102(34):12011-12016.
[32]Mao J Q,Sagraio M.Genomic distribution of three promoters of the bovine gene encoding acetyl-CoA carboxylase and ebidence that the nutritionally regulated promoter Ⅰ contains a repressive element different from that in rat[J].J.Biochem,2001,358:127-135.
[33]Kahn B,Alquier T,Carling D,Hardie D G.AMP-activated protein kinase:Ancient energy gauge provides clues to modern understanding of metabolism[J].Cell Metab,2005,1(1):15-25.
[34]童国玉。固醇调节元件结合蛋白1的研究进展[J]。国外医学内分泌学分册,2002,22:328
[35]Neels JG,Olefsky J M.CELL SIGNALING:A new way to burn fat[J].Science,2006,312:1756-1758.
[36]Spence J T,Pitot HC.Induction of lipogenic enzymes in primary cultures of rat heparocytes relationship between lipogenesis and carbohydrate metabolism[J].Eur.J.Biochem,1982128(1):12-20
[37]Giffhorn S,Katz NR.Glucase-dependent induction of acetyl-CoA carboxylase in rat hepatocyte cultures[J].J.Biochem,1984,221(2):343-350.
[38]O'Callaghan BL,Koo SH.Glucose regulation of the acetyl-CoA carboxylase promoter P1 in rat hepatocytes[J].J.Biochem,2001,276(19):16033-16039.
[39]Salati L M,Clarke SD.Fatty acid inhibition of hormonal induction of acety-CoA carboxylase in hepatocyte monolayers[J].Arch Biochem Biophys,1986,246(1):82-89.
[40]Iritani N,Komiya M.Lipogenic enzyme gene expression is quickly suppressed in rats by a small amount of exogenous polyunsaturated fatty acids[J].J.Nuri,1998,128(6):967-972.
[41]段铭,高宏伟,郝黎明。不同脂肪源对肉仔鸡肝脏中脂肪代谢相关基因表达的影响[J].中国畜牧杂志,2003,39(6):7-9.
[42]Karbowska J,Kochan Z.Increase of lipogenic enzyme mRNA levels in rat white adipose tissue after multiple cycles of starvation-refeeding[J].J.Metabolism,2001,50(6):734-738.
[43]Yin L,Zhang Y.Thyroid hormone,glucagon,and medium-chain fatty acids regulate transcription initiated from promoter1 and promoter2 of the acetyl-CoA carboxylase-alpha gene in chick embryo hepatocytes[J].Biochem Biophys Acta,20001517(1):91-99.
[44]Qi L,Heredia J E,Altarejos J Y.Screaton R,et al.TRB3 Links the E3 Ubiquitin Ligase COP1to Lipid Metabolism[J].Science,2006,312:1763-1766.
[45]Wolfgang M J,Lane M D.The Role of Hypothalamic Malonyl-CoA in Energy Homeostasis[J].J Biol Chem,2006,281:37265-37269
[46]Wolf G.The Regulation of Food Intake by Hypothalamic Malonyl-Coenzyme A:The MalCoA Hypothesis[J].Nutr Rev,2006,64(8):379-384.
[47]Karami K J,Coppola J,Krishnamurthy K,Lianos D J,et al.Effect of food deprivation and hormones of glucose homeostasis on the acetyl CoA carboxylase activity in mouse brain:a potential role of acc in the regulation of energy balance[J].Nutr Metab,2006,3:15
[48]宋庆文.动物脂肪代谢过程中关键酶的研究进展[J].畜牧与饲料科学,2007,3:58-60.
[49]Smith S,Witkowski A,Joshi AK.Structual and functional organization of the animal fatty acid synthase.Progress in Lipid Research,2003,42(4):289-317.
[50]Matthew H H,Chirrda S S,Wakil S.Human fatty-acid synthase gene[J].J Biol Chem.1996,271:13584-13592.
[51]马慧敏.脂肪酸合成酶(FAS)基因的研究进展以及日粮成分对其表达的调控[J].饲料工业, 2007,28(22):60.
[52]Clarke S D.Regulation of fatty acid synthase gene expression:An approach for reducingfat accumulation[J].J An:im Sci.1993,71:1957-1965.
[53]Jayakuumar A,Tai M H,huang W Y,Hsu M.Human fatty acid synthase:proper-ties and molecular cloning[J].Proc Natl Acad sci USA.1995,92(19):8695-8699.
[54]熊文中,杨凤,周安国.猪重组生长激素对不同杂交肥育猪脂肪代谢调控的研究[J].畜牧兽医学报,2001,32(1),1-4
[55]Tomita K,Azumat T,Kitamuran N,et al.Pioglitazone preVents alcohol induced fatty liver in rats through up-regulation of c-met.Gastroenterology,2004,126(3):873-885.
[56]Sato M,Yoshida S,Nagao K,et al.Superiority of dietary safflower oil over olive oil in lowering serum cholesterol and increasing hepatic mRnas for the LDL-C receptor and cholesterol 7alpha-hy-droxylase in exogenously hypercholesterolemic(exHC) rats[J].Biosci Biotechnol Biochem,2000,64:1111-1116.
[57]Marrapodi M,Chiang JY.Peroxisome proliferator-activated receptor alpha(PPA-Ralpha)and agonist inhibit cholesterol 7alpha-hy-droxylase gene(CYP7A1) tra-nscription[J].Lipid Res,2000,41(4):514-520.
[58]Cheema SK.Agellon LB.The murine and human cholesterol 7alpha-hydroxylase gene promoters are differentially responsive to regulation by fatty acids mediated via peroxisome proliferator-activated receptor alpha[J].Biol Chem,2000,275:12530-12536.
[59]Varma A,Palsson B O.Metabolic capabilities of Escherichiacoli.1.Synthesis of biosynthetic precursors and cofactors[J].Theor Biol,1993,165:477-502.
[60]Teusink B,Passarge J,Reijenga C A,Esgalhado E,et al.Can yeast glycolysis be understood in terms of in vitro kinetics of the constituent enzymes?[J].Testing biochemistry.Eur.J.Biochem.,2000,267:5313-5329.
[61]Hatzimanikatis V,Bailey J E.Effects of spatiotemporal variations on metabolic control:approximate analysis using(log)linear kinetic models[J].Biotechnol.Bioeng,1997,54:91-104.
[62]Wang Liqing,Birol L,Hatzimanikatis V.Metabolic control analysis under uncertainly:Framework development and case shudies[J].Biophysical Journal,2004,87:3750-3763
[63]Hardie D G.Regulation of fatty acid synthesis via phosphorylation of acetyl-CoA carboxylase[J].Prog.Lipid Res.,1989,28:117-146.
[64]田维熙,董妍,权晖.不同生长期蛋鸡的体脂水平和肝脏脂肪酸合成酶活性的关系.生物化学杂志,1996,12(2):234-236
[65]Kenneth J.Livak and Thomas D.Schmittgen.Analysis of relative gene expressi-on data using real time quantitative PCR and the 2-△△Ct method,methods 2001,25:402-408.
[66]Colombo J P,Cervantes H,Kokorovic M,Pfister U et al.Effects of dietary protein on the distribution of amino acids in plasma,liver and brain in the rats[J].Ann.Nutr.& Metabol.,1992:36:23-33.
[67]Deschepper K,deGroote G.Effects of dietary'protein,essential and non-essential amino acids on the performance and carcass composition of male broiler chickens[J].British Poult.Sci.,1995,36:229-245
[68]White B D,Dean R G,Martin R J.An association between low levels of dietary protein,elevated NPY gene expression in the basomedial hypothalamus and increased food intake[J].Nutr.Neurosci.,1998,1:173-182.
[69]White B D,He B,Dean R G,Martin R J.Low protein diets increase neuropeptide Y expression in the basomedial hypothalamus of rats[J].J.Nutr,1994,124:1152-1160.
[70]Rothwell N J,Stock M J.Influence of carbohydrate and fat intake on diet-induced thermogenesis and brown fat activity in rats fed low proteindiets[J].J.Nutr,1987,117:1721-172
[71]Zhao X Q,Jorgensen H,Gabert V M,Eggum B O.Energymetabolism and protein balance in growing rats housed in 18℃ or 28℃ environments and fed different levels of dietary protein[J].J.Nutr.,1996,126:2036-2043.
[72]Bikker P,Dirkzwager A,Fledderus J,Trevisi P,et al.The effect of dietary protein and fermentable carbohydrates levels on growth performance and intestinal characteristics in newly weaned piglets[J].J Anim Sci.,2006,84:3337-3345
[73]Beck B,Dollet J M,Max J P.Refeeding after various times of ingestion of a low protein diet:effects on food intake and body weight in rats[J].Physiol.Behav.1989,45:761-765.
[74]Mercer L P,Kelley D S,Humphries L L,Dunn J D.Manipulation of central nervous system histamine or histaminergic receptors(H1) affects food intake in rats[J].J.Nutr.,1994,124:1029-1036.
[75]Schaart M W,Schierbeck H,Schoor S R.Threonine utilization is high in the intestine of piglets[J].J Nutr,2005,135:765-770
[76]刘艳芬,黄银姬,黄晓亮。日粮蛋白水平对0-3周龄肉鸡生产性能和免疫机能的影响[J].中国畜牧兽医,2006,33(3):11-14
[77]顾宪红。断奶仔猪日粮蛋白质需要量及低蛋白日粮对自主的影响[J]。中国农业科技导报,2001,3(1):34-37
[78]Takahashi Y,Kushiro M,Takashi IDE.Effect of dietary protein on adipose tissue gene expression in mice[J].Rep.Nat'l.Food Res.Inst.,2004,68:11-18.
[79]姚国佳,单体中,汪以真,王燕.脂肪酸合成酶基因在皮下脂肪中的表达及其与血清Leptin的关系[J].农业生物技术学报,2006,14(5):818-819
[80]Hagve TA.Effects of unsaturated fatty acid on cell membrane functions.Scandinavian Journal of Clinical and Laboratory Investigation.1988,48:381-8
[81]Thierry Raclot,Hugues Oudart.Selectivity of faty acids on lipid metabolism and gene expression.Proceedings of Nutrition Society,1999,58:633-646
[82]李志琼.α-亚麻酸对产蛋鸡脂质代谢及蛋黄胆固醇沉积的影响及其机理[D].四川农业大学博士论文,2007
[83]余冰.AMPK对应激状态下仔猪脂质代谢的调节作用[D].四川农业大学博士论文,2003
[84]Hardie D G.Regulation of fatty acid synthesis via phosphorylation of acetyl-CoA carboxylase [J].Prog.Lipid Res.,1989,28:117-146.
[85]Wright T C,Cant J P,Brenna J T,McBride B W.Acetyl CoA Carboxylase Shares Control of Fatty Acid Synthesis with Fatty Acid Synthase in Bovine Mammary Homogenate[J].J Dairy Sci,2006,89:2552-2558
[87]Davis M S,Solbiati J,Cronan J E.Overproduction of acetyl-CoA carboxylase activity increases the rate of fatty acid biosynthesis in Escherichia coli[J].J.Biol.Chem,2000,275:28593-28598.
[87]史仍飞,袁海平.运动与能量代谢过程中丙二酸单酰辅酶A的调节[J].天津体育学院学报,2000,15(3):22-24
[88]朱小霞.运动过程中人体骨骼肌脂肪氧化调节的探讨[J].四川体育科学,2005,2:33-40
[89]Winder W W,Arogyasami J,Elayan I M.Muscle malony-CoA decreases during exercise[J].J Appl Physiol,1989,67:2230-2233
[2]Moibi J A,Ekpe E D,and Christopherson R J.Acetyl-CoA carboxylase and fatty acid synthase activity and immunodetectable protein in adipose tissues of ruminants:Effect of temperature and feeding level.J Anim Sci,2000,78:2383-2392
[3]季海峰,张沅.猪脂肪代谢的研究进展[J].中国畜牧杂志,1993,29(3):59-61.
[4]Iritani N,Hosomi H,Fukuda H,Tada K,et al.Soybean protein suppresses hepatic lipogenic enzyme gene expression in Wistar fatty rats[J].J.Nutr,1996,126,380-388.
[5]Iritani N,Suga A,Fukuda H,Katsurada A,et al.Effects of dietary casein and soybean protein on triacylglycerol turnover in rat liver[J]J.Nutr.Sci.Vitaminol,1988,34:309-315.
[6]Shinjo S,Asato L,Arakaki S,Kina T,et al.Comparative effect of casein and soybean protein isolate on body fat accumulation in adult rats[J].J.Nutr.Sci.Vitaminol.,1992,38:247-253.
[7]Aoyama Y,Wada M.Supplementation of orotic acid to the casein,but not to egg protein,soy protein,or wheat gluten diets,increases serum ornithine carbamoyltransferase activity[J].J.Nutr.Biochem.,2000,11:306-310.
[8]Aoyama T,Fukui K,Takamatsu K,Hashimoto Y,et al.Soy protein isolate and its hydrolysate reduce body fat of dietary obese rats and genetically obese mice(Yellow KK)[J].Nutrition,2000,16:349-354.
[9]Ide T,Murata M,Sunada Y.Soybean protein dependent changes in triacylglycerol synthesis and concentration of diacylglycerol in the liver microsomes of fasted-refed rats[J].Ann.Nutr.Metab,1992,36:87-96.
[10]Lapre J A,West C E,Loveti M R,Sirtori C R,et al.Dietary animal proteins and cholesterol metabolism in rats[J].Int.J.Vitam.Nutr.Res,1989,59:93-100.
[11]Iritani N,Sugimoto T,Fukuda H,Komiya M et al.Dietary soybean protein increases insulin receptor gene expression in Wistar fatty rats when dietary polyunsaturated fatty acid level is low[J].J Nutr,1997,127:1077-1083.
[12]Berdanier C D,Kras K M,Wickwire K Hall D G.Whole-egg diet delays the age-related impaired glucose tolerance of BHE/Cdb rats[J].Proc.Soc.Exp.Biol. Med, 1998, 219:28-36(1998).
[13]Mikkelsen P B,Toubro S,Astrup A. Effect of fat reduced diets on 24-h energy expenditure: comparisons between animal protein, vegetable protein, and carbohydrate[J].Am. J. Clin. Nutr,2000,72:1135-1141.
[14]Gannon M C,Nuttall F Q,Neil B J,Westphal S A.The insulin and glucose responses to meals of glucose plus various proteins in type II diabetic subjects[J].Metabloism, 1988,37:1080-1088
[15]Iritani N,Nagashima K,Fukuda H,Katsurada A et al.Effects of dietary proteins on lipogenic enzymes in rat liver[J].J. Nutr, 1986,116:190-197.
[16]Aoki H,Kimura K,Igarashi K,Takenaka A.Soy protein suppresses gene expression of acetyl-coA carboxylase alpha from promoter PI in rat liver[J]. Biosci. Biotechnol. Biochem, 2006, 70(4): 843-849.
[17]Webster A J F.Energy partitioning, tissue growth and appetite control[J].Proc. Nutr. Soc, 1993,52: 69-76.
[18]White B D,He B,Dean R G,Martin R J.Low protein diets increase neuropeptide Y gene expression in the basomedial hypothalamus of rats [J]. J.Nutr,1994, 124:1152 -1160.
[19]Du F Y,Higginbotham D A,White B D.Food intake,energy balance and serum leptin concentrations in rats fed low-protein diets[J].J.Nutr,2000,130:514-521.
[20]Adeola O, Young L G. Dietary protein-induced changes in porcine muscle respiration, protein synthesis and adipose tissue metabolism [J]. J. Anim. Sci, 1989,67:664-673.
[21]Wood J D,S N Brown,G R Nute, F M Whittington,et al.Effects of breed, feed level and conditioning time on the tenderness of pork[J],Meat Sci, 1996, 44: 105-112.
[22] Candek P M,L Lefaucheur,B,Zlender,M Bonneau.Effect of age and/or weight at slaughter on longissimus dorsi muscle: biochemical traits and sensory quality in pigs[J].Meat Sci,1998,48:287-300.
[23] Karlsson A,A C Enfa¨lt, B Essen-Gustavsson, K Lundstro¨m,et al.Muscle histochemical and biochemical properties in relation to meat quality during selection for increased lean tissue growth rate in pigs[J]. J. Anim. Sci, 1993,71: 930-938.
[24]Gondret F,Lebret B. Feeding intensity and dietary protein level affect adipocyte cellularity and lipogenic capacity of muscle homogenates in growing pigs, without modification of the expression of sterol regulatory element binding protein[J].J Anim.Sci,2002.80:3184-3193.
[25]Rosebrough R W,Steele N C.Dietary crude protein levels and the effect of isoproterenol on in vitro lipogenesis in the chicken[J].J.Nutr,1990,120:1684-1691.
[26]Mldner AM,Clarke SD.Porcine fatty acid synthase:Cloning of a complementary DNA,tissue distribution of its mRNA and suppression of expression by somatomin and dietary protein[J].J.Nutr,1991,121:900-907.
[27]Katsurada A,Ritanin,Fukuda H,et al.Effects of nutrients and homones on transcriptional and post-transcriptional regulation of acetyl-CoA carboxylase in rat liver[J].Eur Biochem,190(2):435-441.
[28]McGarry J D,Brown N F.The Mitochondrial Carnitine Palmitoyltransferase System-From Concept to Molecular Analysis[J].Eur J Biochem,1997,244(1):1-14
[29]Clifford D L,Folmes G D,Lopaschuk.Role of malonyl-CoA in heart disease and the hypothalamic control of obesity[J].Cardiovascular Research,2007,73:278-287
[30]White JD.Low protein diets increase neuropetide Y gene expression in the basomedial hypothalamus of rats[J].J Nutr,1994,124:1152-1160.
[31]Abu-Elheiga L,Matzuk M M,Kordari P,Oh W,et al.Mutant mice lacking acetyl-CoA carboxylase Ⅰ are embryonically lethal[J].Proc Natl Acad Sci USA,2005,102(34):12011-12016.
[32]Mao J Q,Sagraio M.Genomic distribution of three promoters of the bovine gene encoding acetyl-CoA carboxylase and ebidence that the nutritionally regulated promoter Ⅰ contains a repressive element different from that in rat[J].J.Biochem,2001,358:127-135.
[33]Kahn B,Alquier T,Carling D,Hardie D G.AMP-activated protein kinase:Ancient energy gauge provides clues to modern understanding of metabolism[J].Cell Metab,2005,1(1):15-25.
[34]童国玉。固醇调节元件结合蛋白1的研究进展[J]。国外医学内分泌学分册,2002,22:328
[35]Neels JG,Olefsky J M.CELL SIGNALING:A new way to burn fat[J].Science,2006,312:1756-1758.
[36]Spence J T,Pitot HC.Induction of lipogenic enzymes in primary cultures of rat heparocytes relationship between lipogenesis and carbohydrate metabolism[J].Eur.J.Biochem,1982128(1):12-20
[37]Giffhorn S,Katz NR.Glucase-dependent induction of acetyl-CoA carboxylase in rat hepatocyte cultures[J].J.Biochem,1984,221(2):343-350.
[38]O'Callaghan BL,Koo SH.Glucose regulation of the acetyl-CoA carboxylase promoter P1 in rat hepatocytes[J].J.Biochem,2001,276(19):16033-16039.
[39]Salati L M,Clarke SD.Fatty acid inhibition of hormonal induction of acety-CoA carboxylase in hepatocyte monolayers[J].Arch Biochem Biophys,1986,246(1):82-89.
[40]Iritani N,Komiya M.Lipogenic enzyme gene expression is quickly suppressed in rats by a small amount of exogenous polyunsaturated fatty acids[J].J.Nuri,1998,128(6):967-972.
[41]段铭,高宏伟,郝黎明。不同脂肪源对肉仔鸡肝脏中脂肪代谢相关基因表达的影响[J].中国畜牧杂志,2003,39(6):7-9.
[42]Karbowska J,Kochan Z.Increase of lipogenic enzyme mRNA levels in rat white adipose tissue after multiple cycles of starvation-refeeding[J].J.Metabolism,2001,50(6):734-738.
[43]Yin L,Zhang Y.Thyroid hormone,glucagon,and medium-chain fatty acids regulate transcription initiated from promoter1 and promoter2 of the acetyl-CoA carboxylase-alpha gene in chick embryo hepatocytes[J].Biochem Biophys Acta,20001517(1):91-99.
[44]Qi L,Heredia J E,Altarejos J Y.Screaton R,et al.TRB3 Links the E3 Ubiquitin Ligase COP1to Lipid Metabolism[J].Science,2006,312:1763-1766.
[45]Wolfgang M J,Lane M D.The Role of Hypothalamic Malonyl-CoA in Energy Homeostasis[J].J Biol Chem,2006,281:37265-37269
[46]Wolf G.The Regulation of Food Intake by Hypothalamic Malonyl-Coenzyme A:The MalCoA Hypothesis[J].Nutr Rev,2006,64(8):379-384.
[47]Karami K J,Coppola J,Krishnamurthy K,Lianos D J,et al.Effect of food deprivation and hormones of glucose homeostasis on the acetyl CoA carboxylase activity in mouse brain:a potential role of acc in the regulation of energy balance[J].Nutr Metab,2006,3:15
[48]宋庆文.动物脂肪代谢过程中关键酶的研究进展[J].畜牧与饲料科学,2007,3:58-60.
[49]Smith S,Witkowski A,Joshi AK.Structual and functional organization of the animal fatty acid synthase.Progress in Lipid Research,2003,42(4):289-317.
[50]Matthew H H,Chirrda S S,Wakil S.Human fatty-acid synthase gene[J].J Biol Chem.1996,271:13584-13592.
[51]马慧敏.脂肪酸合成酶(FAS)基因的研究进展以及日粮成分对其表达的调控[J].饲料工业, 2007,28(22):60.
[52]Clarke S D.Regulation of fatty acid synthase gene expression:An approach for reducingfat accumulation[J].J An:im Sci.1993,71:1957-1965.
[53]Jayakuumar A,Tai M H,huang W Y,Hsu M.Human fatty acid synthase:proper-ties and molecular cloning[J].Proc Natl Acad sci USA.1995,92(19):8695-8699.
[54]熊文中,杨凤,周安国.猪重组生长激素对不同杂交肥育猪脂肪代谢调控的研究[J].畜牧兽医学报,2001,32(1),1-4
[55]Tomita K,Azumat T,Kitamuran N,et al.Pioglitazone preVents alcohol induced fatty liver in rats through up-regulation of c-met.Gastroenterology,2004,126(3):873-885.
[56]Sato M,Yoshida S,Nagao K,et al.Superiority of dietary safflower oil over olive oil in lowering serum cholesterol and increasing hepatic mRnas for the LDL-C receptor and cholesterol 7alpha-hy-droxylase in exogenously hypercholesterolemic(exHC) rats[J].Biosci Biotechnol Biochem,2000,64:1111-1116.
[57]Marrapodi M,Chiang JY.Peroxisome proliferator-activated receptor alpha(PPA-Ralpha)and agonist inhibit cholesterol 7alpha-hy-droxylase gene(CYP7A1) tra-nscription[J].Lipid Res,2000,41(4):514-520.
[58]Cheema SK.Agellon LB.The murine and human cholesterol 7alpha-hydroxylase gene promoters are differentially responsive to regulation by fatty acids mediated via peroxisome proliferator-activated receptor alpha[J].Biol Chem,2000,275:12530-12536.
[59]Varma A,Palsson B O.Metabolic capabilities of Escherichiacoli.1.Synthesis of biosynthetic precursors and cofactors[J].Theor Biol,1993,165:477-502.
[60]Teusink B,Passarge J,Reijenga C A,Esgalhado E,et al.Can yeast glycolysis be understood in terms of in vitro kinetics of the constituent enzymes?[J].Testing biochemistry.Eur.J.Biochem.,2000,267:5313-5329.
[61]Hatzimanikatis V,Bailey J E.Effects of spatiotemporal variations on metabolic control:approximate analysis using(log)linear kinetic models[J].Biotechnol.Bioeng,1997,54:91-104.
[62]Wang Liqing,Birol L,Hatzimanikatis V.Metabolic control analysis under uncertainly:Framework development and case shudies[J].Biophysical Journal,2004,87:3750-3763
[63]Hardie D G.Regulation of fatty acid synthesis via phosphorylation of acetyl-CoA carboxylase[J].Prog.Lipid Res.,1989,28:117-146.
[64]田维熙,董妍,权晖.不同生长期蛋鸡的体脂水平和肝脏脂肪酸合成酶活性的关系.生物化学杂志,1996,12(2):234-236
[65]Kenneth J.Livak and Thomas D.Schmittgen.Analysis of relative gene expressi-on data using real time quantitative PCR and the 2-△△Ct method,methods 2001,25:402-408.
[66]Colombo J P,Cervantes H,Kokorovic M,Pfister U et al.Effects of dietary protein on the distribution of amino acids in plasma,liver and brain in the rats[J].Ann.Nutr.& Metabol.,1992:36:23-33.
[67]Deschepper K,deGroote G.Effects of dietary'protein,essential and non-essential amino acids on the performance and carcass composition of male broiler chickens[J].British Poult.Sci.,1995,36:229-245
[68]White B D,Dean R G,Martin R J.An association between low levels of dietary protein,elevated NPY gene expression in the basomedial hypothalamus and increased food intake[J].Nutr.Neurosci.,1998,1:173-182.
[69]White B D,He B,Dean R G,Martin R J.Low protein diets increase neuropeptide Y expression in the basomedial hypothalamus of rats[J].J.Nutr,1994,124:1152-1160.
[70]Rothwell N J,Stock M J.Influence of carbohydrate and fat intake on diet-induced thermogenesis and brown fat activity in rats fed low proteindiets[J].J.Nutr,1987,117:1721-172
[71]Zhao X Q,Jorgensen H,Gabert V M,Eggum B O.Energymetabolism and protein balance in growing rats housed in 18℃ or 28℃ environments and fed different levels of dietary protein[J].J.Nutr.,1996,126:2036-2043.
[72]Bikker P,Dirkzwager A,Fledderus J,Trevisi P,et al.The effect of dietary protein and fermentable carbohydrates levels on growth performance and intestinal characteristics in newly weaned piglets[J].J Anim Sci.,2006,84:3337-3345
[73]Beck B,Dollet J M,Max J P.Refeeding after various times of ingestion of a low protein diet:effects on food intake and body weight in rats[J].Physiol.Behav.1989,45:761-765.
[74]Mercer L P,Kelley D S,Humphries L L,Dunn J D.Manipulation of central nervous system histamine or histaminergic receptors(H1) affects food intake in rats[J].J.Nutr.,1994,124:1029-1036.
[75]Schaart M W,Schierbeck H,Schoor S R.Threonine utilization is high in the intestine of piglets[J].J Nutr,2005,135:765-770
[76]刘艳芬,黄银姬,黄晓亮。日粮蛋白水平对0-3周龄肉鸡生产性能和免疫机能的影响[J].中国畜牧兽医,2006,33(3):11-14
[77]顾宪红。断奶仔猪日粮蛋白质需要量及低蛋白日粮对自主的影响[J]。中国农业科技导报,2001,3(1):34-37
[78]Takahashi Y,Kushiro M,Takashi IDE.Effect of dietary protein on adipose tissue gene expression in mice[J].Rep.Nat'l.Food Res.Inst.,2004,68:11-18.
[79]姚国佳,单体中,汪以真,王燕.脂肪酸合成酶基因在皮下脂肪中的表达及其与血清Leptin的关系[J].农业生物技术学报,2006,14(5):818-819
[80]Hagve TA.Effects of unsaturated fatty acid on cell membrane functions.Scandinavian Journal of Clinical and Laboratory Investigation.1988,48:381-8
[81]Thierry Raclot,Hugues Oudart.Selectivity of faty acids on lipid metabolism and gene expression.Proceedings of Nutrition Society,1999,58:633-646
[82]李志琼.α-亚麻酸对产蛋鸡脂质代谢及蛋黄胆固醇沉积的影响及其机理[D].四川农业大学博士论文,2007
[83]余冰.AMPK对应激状态下仔猪脂质代谢的调节作用[D].四川农业大学博士论文,2003
[84]Hardie D G.Regulation of fatty acid synthesis via phosphorylation of acetyl-CoA carboxylase [J].Prog.Lipid Res.,1989,28:117-146.
[85]Wright T C,Cant J P,Brenna J T,McBride B W.Acetyl CoA Carboxylase Shares Control of Fatty Acid Synthesis with Fatty Acid Synthase in Bovine Mammary Homogenate[J].J Dairy Sci,2006,89:2552-2558
[87]Davis M S,Solbiati J,Cronan J E.Overproduction of acetyl-CoA carboxylase activity increases the rate of fatty acid biosynthesis in Escherichia coli[J].J.Biol.Chem,2000,275:28593-28598.
[87]史仍飞,袁海平.运动与能量代谢过程中丙二酸单酰辅酶A的调节[J].天津体育学院学报,2000,15(3):22-24
[88]朱小霞.运动过程中人体骨骼肌脂肪氧化调节的探讨[J].四川体育科学,2005,2:33-40
[89]Winder W W,Arogyasami J,Elayan I M.Muscle malony-CoA decreases during exercise[J].J Appl Physiol,1989,67:2230-2233