摘要
近年来,中国迅速发展的海水养殖业面临着病害和养殖环境退化等诸多问
题的困扰,其中对虾养殖业受到白斑综合症病毒(WSSV)的冲击很大。本文针
对这一问题,系统研究了对虾在白斑综合症病毒感染和环境因子胁迫下先天性免
疫因子的动态变化过程,筛选出了能够准确反映对虾健康状况的免疫因子,进而
将其运用到对虾抵抗 WSSV 的机制和环境胁迫对对虾健康状况的影响的研究中。
在对现有文献进行全面综述的基础上,我们通过实验系统比较了中国对虾
和日本对虾在摄食感染 WSSV 后的死亡率、免疫学因子和感染病毒的变化情况,
以及在氨氮胁迫下对虾感染 WSSV 过程中免疫学因子的变化情况。根据各种免
疫学因子的变化情况,从中筛选出与 WSSV 感染直接相关的免疫学因子,并优
选出一氧化氮合成酶(NOS)作为反映对虾抗病力的重要指标,它直接反映出了
两种对虾对白斑综合症病毒抵抗能力的差异,应当是导致其对 WSSV 敏感性差
异的主要原因之一。其水平也受氨氮胁迫的影响。
在此基础上,我们建立了对虾血细胞中诱导型一氧化氮合成酶(iNOS)的
鉴定方法—硝基四氮唑蓝(NBT)还原法和血细胞形态法以及酶活力的测定方法
—L-瓜氨酸分析法和亚硝酸盐分析法并优化了鉴定和测量条件,结果显示: 对虾
血细胞在 4oC 下与 L-精氨酸和 LPS 孵育 8h 以及当 L-精氨酸浓度为 2.5mmol l-1,
LPS 浓度为 100μg ml-1,Ca2+浓度为 2.5mmol l-1 时,iNOS 活力的测定结果最佳。
我们通过NBT还原法和血细胞形态法在中国对虾和日本对虾血细胞中鉴定
到了iNOS,并通过亚硝酸盐法和L-瓜氨酸法对比研究了感染WSSV后这两种对虾
血细胞中iNOS的变化情况,结果显示:在WSSV感染初期,WSSV可以诱导中国
对虾和日本对虾产生iNOS,且日本对虾比中国对虾的iNOS更容易被LPS所诱导。
此后随着病毒的增殖,iNOS活性显著降低,对虾也趋于死亡。iNOS活力的差异
可能是日本对虾较中国对虾对WSSV的抵抗力强的原因之一。
本文在系统比较两种对虾感染WSSV病毒过程中抗病力指标变化的基础上,
优选出与WSSV感染直接相关的抗病力指标,并首次对对虾的一氧化氮合成酶进
行了系统的研究,所得到的结果为深入了解对虾对白班综合症病毒的抗病力机制,
并建立和发展相应的病害防御措施提供了科学依据。
The rapid development of the mariculture industry in China is now facing
the problems such as diseases and degradation of mariculture environment, among
which the shrimp-culture industry is particularly impacted by the disease of white spot
syndrome virus (WSSV). Focusing on this topic, we studied systematically the
variation of immune parameters of Chinese shrimp Fenneropenaeus chinensis and
Kuruma prawn Marsupenaeus japonicus after infection of WSSV by oral route, with
attention of comparing the immunocompetence of the two species to WSSV infection.
The immune parameters were also evaluated in Kuruma prawn M. japonicus under the
stress of ammonia-N and WSSV infection. Based on these works, we discriminated
the immune parameters closely related to WSSV infection from all the parameters
used, and found nitric oxide synthase (NOS) a reliable parameter for indicating
disease resistance ability to WSSV infection.
Based on these findings, we developed nitro blue tetrazolium (NBT) method and
cellular morphological approaches to identify the inducible NOS (iNOS) in the
haemocytes of Kuruma prawn M. japonicus and Chinese shrimp F. chinensis. The
conditions for NBT method were modified to make them suitable for the analysis of
iNOS in shrimp blood cells. Results showed that the optimal concentrations of
L-arginine, lipopolysaccharide (LPS) and Ca2+ for NBT method are 2.5mmol l-1,
100μg ml-1 and 2.5mmol l-1, respectively. Meanwhile, two methods for iNOS activity
assay based on the analysis of nitrite and L-citrulline concentrations were also applied
to study iNOS activity in the haemocytes of shrimp. The results showed that an
incubation time of 8 hours is most suitable for the analysis of iNOS enzyme activity.
The four methods could identify iNOS in shrimp haemocytes and analyze the activity
effectively, thus provide a good way for the further study of role of iNOS in innate
2
对虾抗白斑综合症病毒的免疫学研究
immunology of shrimps.
Furthermore, the iNOS was identified in the haemocytes of Chinese shrimp F.
chinensis and Kuruma prawn M. japonicus, and the methods developed were
employed to evaluate their immunocompetence to WSSV infection in shrimps. The
variations of iNOS activity in both penaeid species post-challenge of WSSV were
assayed with the methods of L-citrulline analysis and nitrite analysis. The result
showed that iNOS activity in WSSV infected Chinese shrimp F. chinensis and
Kuruma prawn M. japonicus increased at 12 and 48 hours post-inoculum, but
decreased sharply after 36 and 72 hours post-inoculum respectively. LPS inducible
NOS activity also decreased significantly with the time elapsed in WSSV infected
shrimps. Results of WSSV detection with the Dig-DNA Probe dot blot hybridization
test showed that WSSV of the two penaeid species couldn’t be detected until iNOS
started decreasing. It can be concluded that WSSV infection can stimulate the iNOS
synthesis in shrimps. However, the enzyme activity decreased sharply after the
proliferation of virus. Therefore, iNOS can be considered as a valuable index for
indicating the health status of Chinese shrimp F. chinensis and Kuruma prawn M.
japonicus. In comparison with Chinese shrimp F. chinensis, the iNOS in the
haemocytes of Kuruma prawn M. japonicus are more inclined to be induced by LPS
after WSSV infection at early stage of infection, which might account for the
relatively longer surviving time of M. japonicus after WSSV infection.
Based on the findings in the comparative study of immune parameters between
Chinese shrimp Fenneropenaeus chinensis and Kuruma prawn Marsupenaeus
japonicus, this thesis firstly carry on the researches of iNOS activity in shrimps, and
find it a reliable parameter for indicating the disease resistance ability in shrimps. The
results here could offer a sound scientific basis for the better understanding of the
mechanism of shrimp disease resistance ability to WSSV infection, as well as the
development of countermeasures for
引文
Acosta F, Ruiz de Galarreta C M, Ellis A E, Díaz R, Gómez V, D Padilla and F Real.
Activation of the nitric oxide response in gilthead seabream after experimental
infection with Photobacterium damselae subsp. Piscicida. Fish & Shellfish
Immunology, 2004(16): 581-588.
Adams A. Response of penaeid shrimp to exposure to Vibrio species. Fish Sellfish
Immunol., 1991(1): 59– 70.
Adams W J, Kimerle R A, & Barnett Jr J W. Sediment criteria and aquatic life
assessment. Environ. Sci. Technol., 1992 (26): 1865–1875.
Adams M S, Crumby W D, Greeley M S Jr, et al. Responses of fish populations and
communities to pulp mill effluents: A holistic assessment. Ecotoxicol. Environ.
Saf, 1992 (24): 347-360.
Adema C, van der Knaap M, Sminia W P W. Molluscan haemocyte mediated
cytotoxicity: the role of reactive oxygen intermediates. Rev. Aquat. Sci., 1991(4):
201–223.
Adler H, Beland J L, Del-Pan NC, Kobzik L, Brewer J P, Martin T R, Rimm I J.
Suppression of herpes simplex virus type 1 (HSV-1)-induced pneumonia in mice
by inhibition of inducible nitric oxide synthase (iNOS, NOS2). J. Exp. Med.,
1997(185): 1533-1540.
Akaike T, & Maeda H. Nitric oxide and virus infection. Immunology,
2000(101):300-308.
Amirante G A. 1986. Agglutinins and lectins of Crustacea. In: Gupta.
A.P.(Ed.),Hemocytic and Hemoral Immunity in Arthropods. Wiley, New York.
pp. 359-380.
Arimoto R, Tripp M P. Characterization of bacterial agglutinin in the hemolymph of
the hard clam Mercenaria meercenaria. J.Invertbr.Pathol. 1977(30): 406-413.
Ashida M, Soderhall K. The prophenoloxidsae activating system in crayfish. Comp.
Biochem. Physiol., 1984(77B):21-26.
93
参考文献
Ashida M, Yamazaki H I. Biochemistry of the Phenoloxidase System In Insects: With
Special Reference to Activation. In: Ishizaki, H. ed., Molting and Metamorphosis.
Springer Verlag Berlin: Japan Sci Soc Press, 1990: 239-263.
Babior B M. Oxidants from phagocytes: agents of defense and destruction. Blood,
1984(64): 959–966.
Bachère E, Destoumieux D, Bulet P. Penaeidins, antimicrobial peptides of shrimp: a
comparison with other effectors of innate immunity. Aquaculture, 2000(191):
71-88.
Bachère E, Miahle E, Rodriguez J. Identification of defence effectors in the
haemolymph of crustaceans with particular reference to the shrimp Penaeus
japonicus (Bate), propects and applications. Fish Shellfish Immunol., 1995a(5):
597-612.
Bachère E, Mialhe E, Noel D, Boulo V, Morvan A, Rodriguez J. Knowledge and
Research Prospects in Marine Mollusk and Crustacean immunology. Aquaculture,
1995b(132): 17-32.
Bachère E, Noel T, Mialhe E. Effects of environmental pollutants and
chemotherapeutic agents on the chemiluminescence of the hemocytes from the
oyster Crassostrea gigas. Eur. Aquacult. Soc., Spec. Publ., Dublin, 1991: 14–15.
Bachère E. Shrimp immunity and disease control - Introduction. Aquaculture, 2000
(191): 3-11.
Barracco M A, Duvic B, Soderhall K. β-1,3-glucan binding protein from the crayfish
Pacifastacus leniusculus, when reached with a β 43 man, induces spreading and
degranulation of crayfish granular cells. Cell Tissue Res., 1991(266): 491-497.
Bauchau A G. Crustaceans. In: Ratcliffe N A and Rowley A F (editors). Invertebrate
blood cells. Academic Press, London and New York, 1981, pp.385-420.
Bell K L, Smith V J. In vitro superoxide production by hyaline cells of the shore crab
Carcinus maenas L. Dev. Comp. Immunol. 1993(17): 211–219.
Benencia F, & Courreges M C. Nitric oxide and macrophage antiviral extrinsic
activity. Immunology, 1999(98): 363–370.
Bi Z and Reiss C S. Inhibition of vesicular stomatitis virus infection by nitric oxide. J.
94
对虾白斑综合症抗病力指标的筛选及其应用的研究
Virol, 1995(69):2208–2213.
Bishop J M, Gosselink J G & Stone. Oxigen consumption and haemolymph
osmolality of brown shrimp, Penaeus aztecus. Fisheries Bulletin, 1980(78):
741-757.
Bradford M M. A rapid and sensitive method for the quantification of microgram
quantities of protein utilizing protein-dye binding. Anal. Biochem.,
1976(72):248-254.
Bray W A, Lawrence A L, Leung-Trujillo J R. The effect of salinity on growth and
survival of Penaeus vannamei, with observations on the interaction of IHHN virus
and salinity. Aquaculture, 1994 (122): 133– 146.
Bredt D S, Hwang P M, Glatt C E, et al. Cloned and expressed nitric oxide
synthasestructurally resembles cytochrome P-450 reductase. Nature, 1991(351):
714–718.
Bredt D S, Snyder S H. Nitric oxide: a physiology messenger molecule. Annu. Rev.
Biochem, 1994(63): 175-195.
Cai S, Huang J, Wang C, Song X, Yu J, Zhang Y, Yang C. Epidermiological studies
on the explosive epidemic disease of prawn in1993-1994. J. Fish. China, 1995(19):
112-117.
Cameron J N. Responses to reversed NH3 and NH+4gradients in a teleost (Ictalurus
punctatus), an elasmobranch (Raia erinacea) and a crustacean (Callineces capidus):
Evidence for NH+4/H+ exchange in the teleost and ealsmobranch. J. exp. Zool.,
1986(239):183-195.
Campbell I L, Samimi A, and Chiang C S. Expression of the inducible nitric oxide
synthase: correlation with neuropathology and clinicalfeatures in mice with
lymphocytic choriomeningitis. J. Immunol, 1994(153):3622–3629.
Cerenius L, Henttonen P, Lindqvist O V, Soderhall K. The crayfish pathogen
Psorospermiiiium-haeckeli activates the Prophenoloxidase activating system of
fresh-water crayfish invitro. Aquaculture, 1991(99): 225-233.
Chakravortty D, Hensel M. Inducible nitric oxide synthase and control of intracellular
95
参考文献
bacterial pathogens. Microbes and Infection, 2003(5): 621–627.
Chang P S, Chen L J, Wang Y C. The effect of ultraviolet irradiation, heat, pH, ozone,
salinity and chemical disinfectants on the infectivity of white spot syndrome
baculovirus. Aquaculture, 1998(166): 1-17.
Chang C F, Su M S, Chen H Y, Liao I C. Dietary β-1,3-glucan effectively improves
immunity and survival of Penaeus monodon challenged with white spot
syndrome virus. Fish & Shellfish Immunology, 2003 (15): 297–310.
Chang C F, Su M S, Chen H Y. A rapid method to quantify total haemocyte count of
Penaeus monodon using ATP analysis. Fish Pathol, 1999(34):211–212.
Chen J C, Chen C T, Cheng S Y. Nitrogen excretion and changes of heocyanin, protein
and amino acid levels in the hemolymph of Penaeus monodon exposed to
different concentrations of ambient ammonia-N at different salinity levels. Mar.
Ecol. Prog. Ser., 1994a (10): 85-94.
Chen J C, Cheng S Y, Chen C T. Changes of haemocyanin, protein and free amino
acid levels in the haemolymph of Penaeus japonicus exposed to ambient
ammonia. Comp. Biochem. Physiol., 1994b(109A): 339-347.
Chen J C, Kou Y Z. Effects of ammonia on growth and molting of Penaeus japonicus
juveniles. Aquaculture, 1992(104): 249-260.
Chen J C, Lin C Y. Effect of ammonia on growth and molting of Penaeus mondon
juveniles. Comp. Biochem. Physiol., 1992a (101C): 449-452.
Chen J C, Lin C Y. Effect of ammonia on growth of Penaeus penicillatus juveniles.
Comp. Biochem. Physiol., 1992b (101C): 443-447.
Chen J C, Lin C Y. Oxygen consumption and ammonia-N excretion of Penaeus
chinensis juveniles exposed to ambient ammonia at different salinity levels.
Comp. Biochem. Physiol., 1992c(102C): 287-291.
Chen J C, Nan F H, Cheng S Y. Effects of ambient ammonia on ammonia-N and
protein concentration in haemolymoh an ammonia-N excretion of Penaeuse
chinensis. Mar. Ecol. Pro. Ser., 1993(98): 203-208.
96
对虾白斑综合症抗病力指标的筛选及其应用的研究
Cheng W, Chen J C. Effects of pH, temperature and salinity on immune parameters of
the freshwater prawn Macrobrachium rosenbergii. Fish Shellfish Immunol.,
2000(10): 387-391.
Cheng W, Chen J C. The virulence of Enterococcus to freshwater prawn
Macrobrachium rosenbergii and its immune resistance under ammonia stress.
Fish& Shellfish Immunology, 2002a(12): 97-109.
Cheng W, Chen J C. Effects of environmental factors on the immune responses of
freshwater prawn Macrobrachium rosenbergii and other decapod crustaceans. J.
Fish. Soc. Taiwan, 2002b(29): 1 –19.
Cheng T C. In vivo effects of heavy metals on cellular defense mechanisms of
Crassostrea virginica: phagocytic and endocytotic indices. J. Invertebr. Pathol.,
1988(51): 215-220.
Cheng T C and Sullivan J T. Effects of heavy metals on phagocytosis by molluscan
hemocytes. Bull. Environ. Res., 1984(138): 155-188.
Cheng T C and Rodirick G E. Lysosome and other enzymes in the haemolymph of
Crassostrea virgiica and Mercenaria mercenaria. Comp. Biochem. Phsiol.,
1975(52B): 443-447.
Chernova O B, Chernov M V, Agarwal M L et al. The role of p53 in regulating
genomic stability when DNA and RNA synthesis are inhibited. Trends Biochem.
Sci., 1995(20): 431–434.
Chisholm J R S, Smith V J. Antibacterial activity in the hemocytes of the shore crab,
Carcinus maenas. J. Mar. Biol. Assoc. U.K., 1992(72): 529-542.
Chotigeat W, Tongsupa S, Supamataya K, Phongdara A. Effect of Fucoidan on
Disease Resistance of Black Tiger Shrimp. Aquaculture, 2004 (233): 23–30.
Chou H Y, Huang C Y, Wang C H, Chiang H C, Lo C F. Pathogenicity of a
baculovirus infection causing white spot syndrome in cultured penaeid shrimp in
Taiwan. Dis. Aquat. Ogan., 1995(23): 165-173.
Cohen R I, Hassell A M, Ye X B, Marzouk K, and Liu S F. Lipopolysaccharide
down-regulates inducible nitric oxide synthase expression in swine heart in vivo.
97
参考文献
Biochemical and Biophysical Research Communications, 2003(307): 451–458.
Cominetti M R, Marques M R F, Lorenzini D M, L?fgren S E, S Daffre and M A
Barracco. Characterization and partial purification of a lectin from the hemolymph
of the white shrimp Litopenaeus schmitti. Developmental & Comparative
Immunology, 2002(26): 715-721.
Conte A, Ottavian E. Nitric oxide synthase activity in molluscan hemocytes. FEBS
Letters, 1995(365): 120-124.
Curtin J F, Donovan M, Cotter T G. Regulation and measurement of oxidative stress
in apoptosis. Journal of Immunological Methods, 2002(265): 49–72.
Cuthbertson B J, Shepard E F, Chapman R W, Gross P S. Diversity of the penaeidin
antimicrobial peptides in two shrimp species. Immunogenetics, 2002(54):
442-445.
Davies S A. Nitric oxide signalling in insects. Insect Biochemistry and Molecular
Biology, 2000 (30): 1123–1138.
Dawson T M, Bredt D S, Fotuhi M, Hwang P M, Snyder S H. Nitric oxide and
NADPH-diaphorase are identical in brain and peripheral tissue. Proc Natl Acad
Sci USA, 1991 (88):7797–7801.
De Groote M A, Testerman T, Xu Y, Stauffer G, Fang FC. Homocysteine antagonism
of nitric oxide-related cytostasis in Salmonella typhimurium. Science, 1996(272):
414–417.
de Murcia G, and de Murcia J M. Poly (ADP-ribose) polymerase: a molecular
nick-sensor. Trends Biochem. Sci., 1994(19): 172–176.
Destoumieux D, Bulet P, Loew D, Dorsselaer AV, Rodriguez J, Bachère E.
Penaeidins, a new family of antimicrobial peptides in the shrimp Penaeus
vannamei (Decapoda). J Biol Chem., 1997(272):28398-28406.
Destoumieux D, Munoz M, Bulet P, Bachère E. Penaeidins, a family of antimicrobial
peptides from penaeid shrimp (Crustacea, Decapoda). Cell. Mol. Life. Sci., 2000a
(57): 1260-1271.
98
对虾白斑综合症抗病力指标的筛选及其应用的研究
Destoumieux D, Munoz M, Cosseau C, Rodriguez J, Bulet P, Comps M, Bachère E.
Penaeidins, antimicrobial peptides with chitin-binding activity, are produced and
stored in shrimp granulocytes and released after microbial challenge. J. Cell. Sci.,
2000b (113): 461-469.
Dikkeboom R, van der Knaap W P W, Meuleman E A. Hemocytes of the pond snail
Lymnaea stagnalis generates reactive forms of oxygen. J. Invertebr. Pathol.,
1987(49): 321–331.
Dimopoulos G, Müller H-M, Levashina E A, et al. Innate immune defense against
malaria infection in the mosquito. Current Opinion in Immunology, 2001(13):
79–88.
Donovan M, Carmody R J, Cotter TG. Light-induced photoreceptor apoptosis in vivo
requires nNOS and guanylate cyclase activity and is caspase-3 independent. J.
Biol. Chem., 2001 (276): 23000-23008
Enoch T and Norbury C. Cellular responses to DNA damage: cell-cycle checkpoints,
apoptosis and the roles of p53 and ATM. Trends Biochem. Sci., 1995 (20):
426–430.
Feelish M R and Noack E. The in vivo metabolism of nitrovasodilators and their
conversion in to vasoactive species. In: Heart Failure-Mechanisms and
Management, eds, Lewis B S and Kimchi A, 1991: 241-255. Springer Verlag,
Berlin.
Flegel T W, Alday-Sanz V. The crisis in Asian shrimp aquaculture: current status and
future needs. J APPL ICHTHYOL., 1998(14): 269-273.
Franchi R I B, Fauquet C M, Knudson D I, Brown F (Eds). Classification and
nomenclature of viruses. Arch. Virol. Suppl., 1991(2): 117-123.
Franchini A, Conte A, Ottaviani E. Nitric oxide: An ancestral immunocyte effector
molecule. Adv. Neuroimmunol, 1995a (5): 463-478.
Franchini A, Fontanili P, Ottaviani E. Invertebrate immunocytes – relationship
between phagocytosis and nitric oxide production. Comp. Biochem. Physiol.,
1995b (110 B): 403-407.
99
参考文献
Friedman N, Tsokos M, Samuni A, et al. Nitric oxide potentiates hydrogen
peroxide-induced killing of Escherichia coli. J. Exp. Med., 1995(182):
1469–1479.
Fries C R and Tripp M R. Depression of phagocytosis in Mercenaria mercenaria
following chemical stress. Dev. Comp. Immunol., 1980 (4): 233-244.
Gaudy R & Slone L. Effect of salinity on oxygen consumption in postlavae of the
penaeid shrimp Peneaus monodon and P. stylirostris without and with
acclimation. Marine Biology, 1981(65): 297-301.
Gillespie J P, Kanost M R, Trenczek T. Biological mediators of insect immunity. Annu.
Rev. Entomol., 1997(42): 611-643.
Gollas-Galván T, Hernández-López J and Vargas-Albores F. Prophenoloxidase from
brown shrimp (Penaeus californiensis) hemocytes. Comparative Biochemistry
and Physiology, 1999(122 B): 77-82.
Gollas-Galván T, Hernández-López J and Vargas-Albores F. Effect of Calcium on the
Prophenoloxidase System Activation of the Brown Shrimp (Penaeus
californiensis, Holmes). Comparative Biochemistry and Physiology, 1997(117A):
419-425.
Gopalakrishna R, Chen Z H and Gundimeda U. Nitric oxide and nitric
oxide-generating agents induce a reversible inactivation of protein kinase C
activity and phorbol ester binding. J. Biol. Chem., 1993(268): 27180–27185.
Gourdon I, Gue?in M-C, Torreilles J, et al. Nitric Oxide Generation by Hemocytes of
the Mussel Mytilus galloprovincialis. NITRIC OXIDE: Biology and Chemistry,
2001(5): 1–6.
Grundy M M, Moore M N, Howell S M and Ratcliffe N A. Phagocytic reduction and
effects on lysosomal membranes by polycyclic aromatic hydrocarbons, in
haemocytes of Mytilus edulis. Aquatic Toxicology, 1996(34): 273-290.
Guan Y Q, Yu Z M and Li C W, The effects of temperature on white spot syndrome
infections in Marsupenaeus japonicus, Journal of Invertebrate Pathology, 2003
(83): 257–260.
100
对虾白斑综合症抗病力指标的筛选及其应用的研究
Hall M, Wang R, Antwerpen R K, Sottrup-Jensen L, S?derh?ll K. The crayfish plasma
clotting: a vitellogenin-related protein responsible for clot formation in
crustacean blood. Proc. Natl. Acad. Sci. U.S.A., 1999(96): 1965-1970.
Hall M R, van Ham E H. The effects of different types of stress on blood glucose in
the giant tiger prawn Penaeus monodon. Journal of the world aquaculture society,
1998(29): 290-299.
Hervio D, Bachère E, Mialhe E, et al. Chemiluminescent responses of Ostrea edulis
and Crassostrea gigas hemocytes to Bonamia ostrea Ascetospora. Dev. Comp.
Immunol., 1989(13): 449.
Hoffmann J A, Kafatos F C, Janeway Jr C A, Ezekowitz R A B. Phylogenetic
perspectives in innate immunity. Science, 1999(284):1313–1318.
Hogg N, Kalyanaraman B. Nitric oxide and lipid peroxidation. Biochimica et
Biophysica Acta, 1999(1411): 378-384.
Holmblad T, S?derh?ll K. Cell adhesion molecules and antioxidative enzymes in a
crustacean, possible role in immunity. Aquaculture, 1999(172): 111–123.
Holscher, C. Nitric oxide, the enigmatic neuronal messenger: its role in synaptic
plasticity. Trends Neurosci., 1997(20): 298–303.
Hope B T, Michael G J, Knigge K M, Vincent S R. Neuronal NADPH diaphorase is a
nitric oxide synthase. Proc Natl Acad Sci USA, 1991(88):2881–2814.
Hose J E, Lightne D V, Redman R M. and Danald D A. Observations on the
pathogenesis of the imperfect fungus, Fusarium solani, in the Californian brown
shrimp, Penaeus californiensis. Journal of Invertebrate Pathology, 1984 (44):
292-303.
Howe D, Barrows L F, Lindstrom N M, Heinzen R A. Nitric oxide inhibits Coxiella
burnetii replication and parasitophorous vacuole maturation. Infect. Immun.,
2002(70): 5140-5147.
Huang J, Song X L, Yu J, Zhang L J. The components of an inorganic physiological
buffer for Penaeus chinensis. Methods Cell Sci. 1999(21): 225–230.
Incze K, Farkas J, Mihalyi V, et al. Antibacterial effect of cysteine-nitrosothiol and
possible percursors thereof . Appl. Microbiol., 1974(27): 202–205.
101
参考文献
Itami T, Takahasshi Y, Tsuchihiea E, Igusa H, Kondo M. Enhancement of disease
resistance of kuruma shrimp, Penaeus japonicus and increase in phagocytic
activity of prawn hemocytes after oral administration of β-1,3-glucan
(Schizophyllan). In: Chou, L.M., Munro, A.D., Lam, T.J., Chen, T.W., Cheong,
L.K.K., Ding, J.K., Hooi, K.K., Khoo, H.W., Phang, V.P.E., Shim, K.F., Tan,
C.H.(Eds.),The 3rd Asian Fisheries Forum. Asian Fisheries Society, Manila,
Philippines, 1994, pp. 375-378.
James F, Curtin L, Maryanne D, et al. Regulation and measurement of oxidative stress
in apoptosis. Journal of Immunological Methods, 2002(265): 49–72.
Johansson M W, S?derh?ll K. Cellular immunity in crustaceans and the proPO system.
Parasitol Today, 1989(5):171–176.
Kandemir O, Polat A, and Kaya A, Inducible nitric oxide synthase expression in
chronic viral hepatitis and its relation with histological severity of disease.
Journal of Viral Hepatitis, 2002(9): 419–423.
Kang C J, Wang J X, Zhao X, Yang X M, Shao H L and Xiang J H. Molecular cloning
and expression analysis of Ch-penaeidin, an antimicrobial peptide from Chinese
shrimp, Fenneropenaeus chinensis. Fish & Shellfish Immunology, 2004(16):
513-525
Karupiah, G., et al. Inhibition of Viral Replication by Interferon-γ-Induced Nitric
Oxide Synthase. Science, 1993 (261): 1445-1448.
Kautsky N, Ronnback P, Tedengren M, Troell M. Ecosystem perspectives on
management of disease in shrimp pond farming. Aquaculture, 2000(191):
145-161.
Kawabata S I, Muta T, Iwanaga S. The clotting cascade and defense molecules found
in the hemolymph of the horseshoe crab. In: Soderhall, K., Iwanaga, S., Vasta,
G.R. (Eds.), New Directions in Invertebrate Immunology. SOS Publications,
Fair Haven, 1996, pp. 255-283.
Kandemir ?, Polat A and Kaya A Inducible nitric oxide synthase expression in
chronic viral hepatitis and its relation with histological severity of disease.
Journal of Viral Hepatitis, 2002(9): 419–423.
102
对虾白斑综合症抗病力指标的筛选及其应用的研究
Kiemer A K, Müller C and Vollmar A M. Inhibition of LPS-induced nitric oxide and
TNF-α production by α-lipoic acid in rat Kupffer cells and in RAW 264.7
murine macrophages. Immunology and Cell Biology, 2002(80): 550–557.
Kim Y M, Bergonia H A, Muller C, et al. Loss and degradation of enzyme-bound
heme induced by cellular nitric oxide synthesis. J. Biol. Chem., 1995(270):
5710–5713.
KimY J, Choi W C, Kim H R, Jung S J, Oh M J. Changes in Penaeus chinensis
haemocytes during white spot baculovirus (WSBV) infections. Bull. Eur. Assn.
Fish. P., 1999(19): 213-215.
Kinne O. Cultivation of marine organism: Water quality management and technology.
In Marine Ecology, 1976, Vol. Ⅲ , Part 1 (Edited by Kinne O.), pp.
79-300.Wiley-Interscience, New York.
Kitano T, Matsumura S, Seki T, Hikida T, Sakimura K, Naganoe T, Mishina M,
Nakanishi S, Ito S. Characterization of N-methyl-d-aspartate receptor subunits
involved in acute ammonia toxicity. Neurochemistry International, 2004(44):
83–90
Kopacek P, Hall M, S?derh?ll K. Characterization of clotting of a clotting protein
isolated from plasma of the fresh crayfish Pacifastacus leniusculus. Eur. J.
Biochem., 1993(62): 459-463.
Kou Y Z, Chen J C. Acute toxicity off ammonia to Penaeus japonicus Bate juveniles.
Aquacult. Fish. Manage., 1991(22): 259-263.
Kreil T R, and Eibl M M. Nitric oxide and viral infection: no antiviral activity against
a flavivirus in vitro and evidence for contribution to pathogenesis in
experimental infection in vivo. Virology, 1996(219):304–306.
Kr?ncke K D, Fehsel K, and Kolb-Bachofen V. Nitric Oxide: Cytotoxicity versus
Cytoprotection—How, Why, When, and Where?. NITRIC OXIDE: Biology and
Chemistry, 1997(1): 107–120.
Lackie A M. Invertebrate immunity. Parasitology, 1980(80): 393-412.
Laing K J, Grabowski P S, Belosevic M, Secombes C J. A partial sequence for nitric
oxide synthase from a goldfish Carassius auratus macrophagecell line.
103
参考文献
Immunol. Cell Biol., 1996, (74): 374-379.
Laing K J, Hardie L J, Aartsen W, et al. Expression of an inducible nitric oxide
synthase gene in rainbow trout Oncorhynchus mykiss. Developmental and
Comparative Immunology, 1999, (23): 71-85.
Lane T E, Paoletti A D, and Buchmeier M J. Disassociation between the in vitro and
in vivo effects of nitric oxide on a neurotropic murine coronavirus. J. Virol,
1997(71): 2202–2210.
Larson K G, Roberson B S, Hetrick F M. Effect of environmental pollutants on the
chemiluminescence of hemocytes from the American oyster Crassostrea
?irginica. Dis. Aquat. Org., 1989(6): 131–136.
Le Gall G, Bachére E, Mialhe E. Chemiluminescence analysis of the activity of
Pecten maximus hemocytes stimulated with zymosan and host-specific
Rickettsiales-like organisms. Dis. Aquat. Org., 1991(11): 181–186.
Leake L D, Moroz L L.Putative nitric oxide synthase (NOS)-containing cells in the
central nervous system of the leech, Hirudo medicinalis: NADPH-diaphorase
histochemistry. Brain Research, 1996(723): 115-124.
Lee S Y, Soderhall K. Early events in crustacean innate immunity. Fish & Shellfish
Immunology, 2002(12): 421-437.
LeMoullac G, LeGroumellec M, Ansquer D, Froissard S, Levy P, Aquacop.
Haematological and phenoloxidase activity changes in the shrimp Penaeus
stylirostris in relation with the moult cycle: Protection against vibriosis. Fish
Shellfish Immunol., 1997 (4): 227-234.
LeMoullac G, Soyez C, Saulnier D, Ansquer D, Avarre J C, Levy P. Effect of hypoxic
stress on the immune response and the resistance to vibriosis of the shrimp
Penaeus stylirostris. Fish & Shellfish Immunology, 1998 (8): 621-629.
LeMoullac G, Haffner P. Environmental factors affecting immune responses in
Crustacea. Aquaculture, 2000(191): 121-131.
Lepoivre, M., et al. Alterations of Ribonucleotide Reductase Activity Following
Induction of the Nitrite-generating pathway in adenocarcinoma cell. J. Biol.
Chem., 1990(265):14143-14149.
104
对虾白斑综合症抗病力指标的筛选及其应用的研究
Lightner D V. A Handbook of Shrimp Pathology and Diagnostic Procedures for
Diseases of Cultured Penaeid Shrimp. World Aquaculture Society, Baton Rouge,
1996, pp. 305. L A.
Lightner D V, Redman R M. Strategies for the control of viral diseases of shrimp in
the Americas. Fish. Pathol., 1998(33):165-180.
Lindahl T, Satoh M S, Poirier G G, and Klungland A. Post-translational modification
of poly(ADP-ribose) polymerase induced by DNA strand breaks. Trends
Biochem. Sci, 1995(20): 405–411.
Liu C-H, Chen J-C. Effect of ammonia on the immune response of white shrimp
Litopenaeus vannamei and its susceptibility to Vibrio alginolyticus. Fish &
Shellfish Immunology, 2004(16): 321–334.
López-Guerrero J A, Carrasco L. Effect of nitric oxide on poliovirus infection of two
human cell lines. J. Virol., 1998(72): 2538-2540.
Luckhart S, Li K.Transcriptional complexity of the Anopheles stephensi nitric oxide
synthase gene. Insect Biochemistry and Molecular Biology, 2001(31): 249–256.
Luckhart S, Rosenberg R. Gene structure and polymorphism of an invertebrate nitric
oxide synthase gene. Gene, 1999(232): 25–34.
Luckhart S, Vodovotz Y, Cui L, and Rosenberg R. The mosquito Anopheles stephensi
limits malaria parasite development with inducible synthesis of nitric oxide.
Proc. Natl. Acad. Sci. USA, 1998(95): 5700–5705.
Mabuchi T, Matsumura S, Okuda-Ashitaka E, Kitano T, Kojima T, Nagano T,
Minami T, Ito S. Attenuation of neuropathic pain by nociceptin/orphanin FQ
antagonist is mediated by inhibition of nitric oxide production. Eur. J. Neurosci.,
2003 (171): 384–1392.
Maheswari R, P Mullainadhan and M Arumugam. Isolation and characterization of an
acetyl group-recognizing agglutinin from the serum of the Indian white shrimp
Fenneropenaeus indicus. Archives of Biochemistry and Biophysics, 2002(402):
65-76.
105
参考文献
Malagoli D, Conte A, Ottaviani E. Protein kinases mediate nitric oxide-induced
apoptosis in the insect cell line IPLB-LdFB. Cell. Mol. Life. Sci., 2002(59):
894-901.
Marletta M A. Nitric oxide: biosynthesis and biological significance.Trends Biochem.
Sci., 1989(14): 488–492.
Marques M R F, Barracco M A. Lectins, as non-self-recognition factors, in
crustaceans. Aquaculture, 2000(191): 23-44.
Martin G G, Hose J E, Omori S, Chong C, Hoodbhoy T, McKrell N. Localization and
roles of coagulogen and transglutaminase in hemolymph coagulation in decapod
crustaceans. Comp Biochem Physiol, 1991(100B):517–22.
Marzinzig M, Nussler A K, Stadler J, Marzinzig E, Barthlen W, Nussler N C, Beger H
G, Morris Jr S M and Bruckner U B. Improved Methods to Measure End
Products of Nitric Oxide in Biological Fluids: Nitrite, Nitrate, and S-Nitrosothiols.
NITRIC OXIDE: Biology and Chemistry, 1997(1): 177–189.
Mayer B. Regulation of nitric oxide synthase and soluble guanylate cylase. Cell.
Biochem. Function, 1994(12): 167–177.
Montano-Perez K, Yepiz-Plascencia G, Higuera-Ciapara I, Vargas-Albores F.
Purification and characterization of the clotting protein from the white shrimp
Penaeus vannamei . Comp. Biochem. Physiol., 1999(122B): 381-387.
Moormann A M, Koenig R J, and Meshnick S R. Effects of hydrogen peroxide, nitric
oxide and antioxidants on NF-kappa B. Redox Report, 1996(2): 249–256.
Mulero V and J Meseguer. Functional characterisation of a macrophage-activating
factor produced by leucocytes of gilthead seabream (Sparus aurata L.). Fish &
Shellfish Immunology, 1998, 8: 143-156.
Mulcahy M F, Twomey E, Peterson A and Maye C T. Pathobiology of estuarine fish
and shellfish in relation to pollution. In: Biological Indicators of Pollution,
edited by D.H.S. Richardson. Royal Irish Academy, Dublin, (1987) pp.
201-210.
Mu?oz M, Cede?o R, Rodríguez J, et al. Measurement of reactive oxygen
intermediate production in haemocytes of the penaeid shrimp, Penaeus
106
对虾白斑综合症抗病力指标的筛选及其应用的研究
Vannamei. Aquaculture, 2000(191): 89–107.
Mu?oz M, Vandenbulcke F, Saulnier D, Bachère E. Expression and distribution of
penaeidin antimicrobial peptides are regulated by haemocyte reactions in
microbial challenged shrimp. Eur. J. Biochem., 2002(269): 2678-2689.
Nakatsuboa N, Kojimaa H, Kikuchia K, Nagoshib H, Hiratab Y, Maedaa D, Imaia Y,
Irimuraa T, Naganoa T. Direct evidence of nitric oxide production from bovine
aortic endothelial cells using new fluorescence indicators: diaminofluoresceins
FEBS Letters, 1998(427): 263-266.
Nappi A J, Vass E, Frey F, et al. Nitric Oxide Involvement in Drosophila Immunity.
NITRIC OXIDE: Biology and Chemistry, 2000 (4): 423-430.
Naviner M, Berge J-P, Durand P, LeBris H. Antibacteral activit of marine diatom
Skeletonema costatum against aquacultural pathogens. Aquaculture, 1999 (174):
15-24.
Neumann N F, Belosovic M. Deactivation of primed respiratory burst response of
goldfish macrophages by leukocyte-derived macrophage activating factor(s).
Developmental and Comparative Immunology, 1996(20): 427-439.
Neumann N F, D R Barreda and Miodrag Belosevic. Generation and functional
analysis of distinct macrophage sub-populations from goldfish (Carassius
auratus L.) kidney leukocyte cultures. Fish & Shellfish Immunology, 2000, 10(1):
1-20.
Neumeister B, Bach W, Faigle M, et al. Induction of NOS in human monocytes
infected with different Legionella species. FEMS Microbiol Letters, 2001(202):
31-38 .
Nishida K J, Ohta Y S J and Ishiguro I. Relation of Inducible Nitric Oxide Synthase
Activity to Lipid Peroxidation and Nonprotein Sulfhydryl Oxidation in the
Development of Stress-Induced Gastric Mucosal Lesions in Rats. Nitric Oxide,
1998 (2): 215-223.
Novas A, Cao A, Barcia R, et al. Nitric oxide release by hemocytes of the mussel
Mytilus galloprovincialis Lmk was provoked by interleukin-2 but not by
lipopolysaccharide. The International Journal of Biochemistry & Cell Biology,
107
参考文献
2004(36): 390-394.
Olafsen J A. Invertebrate lectins: biochemical heterogeneity as a possible key to their
biological function. In: Breh é lin. M (Ed.), Immunity in Invertebrates.
Springer-Verlag, Berlin. 1986, pp.94-111.
Olafsen J A. Role of lectins in invertebrate humoral defence. Am. Fish. Soc. Sp. Publ.,
1988(18): 189-205.
Ott S R, and Burrows M. NADPH Diaphorase Histochemistry in the Thoracic Ganglia
of Locusts, Crickets, and Cockroaches: Species Differences and the Impact of
Fixation. The Journal of Comparative Neurology, 1999(410):387–397.
Ottaviani E, Barbieri D, Malagoli D, Franchini A. Nitric oxide induces apoptosis in
the fat body cell line IPLB-LdFB from the insect Lymantria dispar. Comparative
Biochemistry and Physiology, 2001(128B): 247-254.
Ottaviani E, Franchini A, Cassanelli S, et al. Cytokines and invertebrate immune
responses . Biol. Cell., 1995(85): 87-91.
Ottaviani E, Paeman L R, Cadet P, et al. Evidence for nitric oxide production and
utilization as a bacteriocidal agent by invertebrate immunocytes. Eur. J.
Pharmacol., 1993 (248): 319-324.
Ouellet H, Ouellet Y, Richard C, Labarre M, Wittenberg B, Wittenberg J, Guertin M.
Truncated hemoglobin HbN protects Mycobacterium bovis from nitric oxide. Proc.
Natl. Acad. Sci. USA, 2002 (99): 5902–5907.
Pacelli R, Wink D A, Cook J A, Krishna M C, DeGraff W, Friedman N, Tsokos M,
Samuni A, Mitchell J B. Nitric oxide potentiates hydrogen peroxide-induced
killing of Escherichia coli, J. Exp. Med. 1995 (182): 1469–1479.
Palmer R M, Ferrige A G, Moncada S. Nitric oxide release accounts for the biological
activity of endothelium-derived relaxing factor. Nature, 1987(327): 524–526.
Panikkar N K. Osmotic behaviour of shrimps and prawn\ in relation to their biology
and culture. FAO Fish. Rep., 1968(57): 527-538.
Peng S E, Lo C F, Ho C H, Chang C F, Kou G H. Detection of white spot baculovirus
(WSBV) in giant freshwater prawn, Macrobrachium rosenbergii, using
polymerase chain reaction. Aquaculture, 1998(164): 253-262.
108
对虾白斑综合症抗病力指标的筛选及其应用的研究
Pequeux A and Gills R. Na+ fluxes across isolated perfused gills off the Chinese crab
Eriocheir sinensis. J. exp. Biol., 1981(92): 173-186.
Persson M, Cerenius L, Soderhall K. The influence of haemocytes number on the
resistance of the freshwater crayfish, Pacifatacus leniusculus Dana, to the
parasitic fungus Aphanomices astaci. J. Fish Dis., 1987(10): 471-477.
Pfarr K M, Qazi S, and Fuhrman J A. Nitric oxide synthase in Filariae: Demonstration
of nitric oxide production by embryos in Brugia malayi and Acanthocheilonema
viteae. Experimental Parasitology, 2001(97): 205-214.
Pickwell G V and Steinert S A Serum biochemical and cellular responses to
experimental cupric ion exchange in mussels. Mar. Environ. Res., 1984 (14):
245-265.
Prayitno S B, Latchford J W. Experimental infections of crustaceans with luminous
bacteria related to Photobacterium and Vibrio. Effect of salinity and pH on
infectiosity. Aquaculture, 1995 (132): 105-l 12.
Pressley T A, Graves J S, Krall A R. Amiloride-sensitive ammonium and sodium ion
transport in the blue crab. Am. J. physiol., 1981(241): 370-378.
Quaroni L, Reglinski J, Wolf R, et al. Interaction of nitrogen monoxide with
cytochrome P-450 monitored by surface-enhanced resonance Raman scattering.
Biochim. Biophys. Acta, 1996(1296): 5–8.
Raber J, Toggas S M, Lee S, Bloom F E, Epstein C J, and Mucke L. Central nervous
system expression of HIV-1 Gp120 activates the hypothalamic pituitary-adrenal
axis: evidence for involvement of NMDA receptors and nitric oxide synthase.
Virology, 1996(226):362–373.
Regnault M. Nitrogen excretion in marine and freshwater-water crustacea. Bio. Rev.,
1987(62): 1-24.
Regulski M and Tully T. Molecular and biochemical characterization of dNOS: A
Drosophila Ca2+/calmodulindependent nitric oxide synthase. Proc. Natl. Acad. Sci.
USA, 1995(92): 90722–90726.
Reiss C S and Komatsu T. Does Nitric Oxide Play a Critical Role in Viral Infections? .
J. Virology, 1998(72): 4547–4551.
109
参考文献
Reyes-Izquierdo T and Vargas-Albores F. Proteinase Activity in the White Shrimp
(Penaeus vannamei) Clotting Protein. Biochemical and Biophysical Research
Communications, 2001(287): 332-336.
Robinson N M K, Zhang H Y, Bevan A L, de Belder A J, Moncada S, Martin J F, and
Archard L C. Induction of myocardial nitric oxide synthase by Coxsackie B3
virus in mice. European Journal of Clinical Investigation, 1999(29): 700–707.
Roch P. Defense mechanisms and disease prevention in farmed marine invertebrates.
Aquaculture, 1999(172): 125-145.
Rodríguez J, Le Moullac G. State of the art of immunological tools and health control
of penaeid shrimp. Aquaculture, 2000(191): 109-119.
Rolff J and Siva-Jothy M T. Invertebrate Ecological Immunology. Science,
2003(301):472-475.
Rojtinnakorn J, Hirono I, Itami T, Takahashi Y, Aoki T. Gene expression in haemocytes
of kuruma prawn, Penaeus japonicus, in response to infection with WSSV by EST
approach. Fish & Shellfish Immunology, 2002(13): 69-83.
Rosselli M, Keller P J, Dubey R K. Role of nitric oxide in the biology, physiology and
pathophysiology of reproduction. Human Reprod. Update, 1998(4): 3–24.
Saeij J PJ, Stet R J M, Groeneveld A, Verburg-van Kemenade B M L, van-Muiswinkel
W B, Wiegertjes G F. Molecular and functional characterization of a fish
inducible-type nitric oxide synthase. Immunogenetics, 2000(51): 339-346.
Sahul Hameed A S, Xavier C M, Anilkumar M, Tolerance of Macrobrachium
rosenbergii to white spot syndrome virus. Aquaculture, 2000(183): 207–213.
Saleh D, Barnes P J, Giaid A. Increased prodiction of the potent oxidant peroxynitrite
in the lungs of patients with idiopathic pulmonary fribrosis. Am J Crit Care Med,
1997(155):1763-1769.
Salzet M, Breton C, Bisogno T, and Marzo V D. Comparative biology of the
endocannabinoid system-possible role in the immune response. Eur. J. Biochem,
2000(267): 4917-4927.
Sami S, Faisal M and Huggett R J. Effects oflaboratory exposure to sediments
contaminated with polycyclic aromatic hydrocarbons on hemocytes of the
110
对虾白斑综合症抗病力指标的筛选及其应用的研究
American oyster, Crassostrea virginica. Mar.Environ. Res., 1993 (35): 131-135.
Sanders S P, Sierkierski E S, Porter J D, Richards S M, and Proud D. Nitric oxide
inhibits rhinovirus-induced cytokine production and viralreplication in a human
respiratory epithelial cell line. J. Virol, 1998(72): 934–942.
Scharsack J P, Steinhagen D, Kleczka C, Schmidt J O, Korting W, Michael R D,
Leibold W, and Schuberth H J.The haemoflagellate Trypanoplasma borreli
induces the production of nitric oxide, which is associated with modulation of
carp (Cyprinus carpio L.) leucocyte functions. Fish & Shellfish Immunology,
2003 (14): 207–222.
Schulz S, Chinkers M, Garbers D L. The guanylate cyclase/receptor family of proteins.
FASEB J., 1989(3): 2026–2035.
Secombes C J, Wang T, Hong S, Peddie S, Crampe M, Laing K J, Cunningham C, Zou
J. Cytokines and immunity of fish. Developmental and Comparative Immunology,
2001(25): 713-723.
Sellers V M, Johnson M K and Dailey H A. Function of the [2FE-2S] cluster in
mammalian ferrochelatase: a possible role as a nitric oxide sensor. Biochemistry,
1996(35): 2699–2704.
Selvin J, Huxley A J, Lipton A P.Immunomodulatory potential of marine secondary
metabolites against bacterial diseases of shrimp. Aquaculture, 2004 (230):
241–248.
Sharon N, Lis H. Lectin: Cell—agglutinin and sugar—specific proteins. Science,
1972(177): 949-959.
Sindermann C J. Pollution associated diseases and abnormalities of fish and shellfish:
a review. Fish. Bull., 1979(76): 717-749.
Sindermann C J and Rosenfield A. Principal diseases of commercially important
bivalve mollusks and crustaceans. Fish. Bull., 1967 ( 66): 335-384.
Smith V J. Invertebrate immunology: phylogenetic, ecotoxicological and biomedical
implications. Comp. Haematol. lnt., 1991 (1): 61-76.
Smith V J, Brown J H and Hauton C. Immunostimulation in crustaceans: does it really
111
参考文献
protect against infection?. Fish & Shellfish Immunology, 2003(15): 71-90.
Smith V J, Johnston P A. Differential hemotoxic effect of PCB congeners in the
common shrimp, Crangon crangon. Comp. Biochem. Physiol., 1992(101C):
641-649.
Smith V J, S?derh?ll K. A comparison of phenoloxidase activity in the blood of
marine invertebrates. Dev. Comp. Immunol., 1991(15): 251-261.
Smith V J, Swindlehurst R J, Johnston P A, Vethaak A D. Disturbance of host defense
capability in the common shrimp, Crangon crangonI, by exposure to harbor
dredge spoils. Aquatic Toxicology, 1995(32): 43-58.
S?derh?ll K. Fungal cell wall β-1,3-glucans induce clotting and phenoloxidase
attachment to foreign surface of crayfish hemocyte lysate. Dev. Comp.
Immunol., 1981(5): 565-573.
S?derh?ll K. Editorial. Dev. Comp. Immunol., 1999(23): 263-266.
S?derh?l K, Cerenius L. Crustacean immunity. Annual Review of Fish Disease,
1992(2):3-23.
S?derh?l K, Cerenius L. Role of the prophenoloxidase-activating system in
invertebrate I- mmunity. Curr. Opin. Immunol, 1998(10): 23-28.
S?derh?l K, Hall L. Lipopolysaccharide induced actvation of the prophenoloxidase
activating system in crayfish haemocyte. Biochem. Biophys. Acta., 1984(797):
99-104.
S?derh?l K, Smith V J. Separation of the haemocyte of populations of Carcinus
maenas and other marine decapods, and prophenoloxidase distribution. Dev.
Comp. Immunol, 1983(7): 229-239.
S?derh?l K, Smith V J. The prophenolaxidase activating system: the biochemistry of
its activating and role in arthropod cellular immunity with special reference to
Crustacea. In Breheline, M. (Ed.), Immunity in Invertebrates.
Springer-Verlag.Berlin, 1986, pp.208-223.
Song Y L, Hsieh Y T. Immunostmulation of tiger shrimp (Penaeus monodon)
hemocytes for generation of microbicidal substances-analysis of reactive oxygen
species. Dev. Comp. Immunol, 1994(18): 201-209.
112
对虾白斑综合症抗病力指标的筛选及其应用的研究
Song Y L, Yu C-I, Lien T-W, Huang C-C and Lin M-N. Haemolymph parameters of
Pacific white shrimp (Litopenaeus vannamei) infected with Taura syndrome virus.
Fish & Shellfish Immunology, 2003(14): 317-331.
Sritunyalucksana K, Cerenius L and S?derh?ll K. Molecular cloning and
characterization of prophenoloxidase in the black tiger shrimp, Penaeus monodon.
Developmental & Comparative Immunology, 1999(23): 179-186.
Sritunyalucksana K, Sithisarn P, Withayachumnarnkul B and Flegel T W. Activation of
prophenoloxidase, agglutinin and antibacterial activity in haemolymph of the
black tiger prawn, Penaeus monodon, by immunostimulants. Fish & Shellfish
Immunology, 1999a (9): 21-30.
Sritunyalucksana K, S?derh?ll K. The proPO and clotting system in crustaceans.
Aquaculture, 2000(191): 53-69.
Stefano G B, Ottaviani E. The biochemical substrate of nitric oxide signaling is
present in primitive non-cognitive organisms. Brain. Res., 2002(924): 82-89.
Stewart J E, Arie B, Zwicker B M and Dingle J R. Gaffkemia, a bacterial disease of
the lobster, Homarus americanus: effects of the pathogen, Gaffkemia homari, on
the physiology of the host. Canadian Journal of Microbiology 1969 (15): 925-932.
Sung H H, Chang H J, Her C H, Chang J C and Song Y L. Phenoloxidase Activity of
Hemocytes Derived from Penaeus monodon and Macrobrachium rosenbergii.
Journal of Invertebrate Pathology, 1998(71): 26-33.
Suresh K and Mohandas A. Hemolymph acid phosphatase activity pattern in copper
stressed crabs. J. Invertebr. Pathol., 1990 (55): 118-125.
Tafalla C, Gómez-León J, Novoa B, et al. Nitric oxide production by carpet shell clam
(Ruditapes decussatus) hemocytes. Developmental and Comparative
Immunology, 2003(27): 197–205.
Tafalla C, Novoa B. Requirements for nitric oxide production by turbot
(Scophthalmus maximus ) head kidney macrophages. Developmental and
Comparative Immunology, 2000(24): 623-631.
Tafalla C, Figueras A, Novoa B. Role of nitric oxide on the replication of
haemorrhagic septicemia virus (VHSV), a fish rhabdovirus. Veterinary
113
参考文献
Immunology and immunopathology, 1999(72): 249-256.
Torreilles J and Gueurin M C. Production of peroxynitrite by zymosan stimulation of
Mytilus galloprovincialis haemocytes in vitro. Fish & Shellfish Immunology,
1999(9): 509–518.
Truscott R, White K N. The influence of metal and temperature stress on the immune
system of crabs. Funct. Ecol., 1990 (4): 455–461.
Vaandrager A B, De Jonge H R. Signalling by cGMP-dependent protein kinases. Mol.
Cell. Biochem., 1996(157): 23–30.
Van Hulten M C, Wittevel Peters S, Kloosterboer N, Tarchini R, Fiers M, Sandbrink
H, Lankhorst R K, Vlak J M. The white spot syndrome virus DNA genome
sequence. Virology, 2001(286): 7– 22.
Van de Braak C B T, Botterblom M H A, Huisman E A, et al. Preliminary study on
haemocyte response to white spot syndrome virus infection in black tiger
shrimp Penaeus monodon. Dis. aquat. organ., 2002(51): 149-155.
Vargas-Albores F, Guzmán M A, Ochoa J L. An anticoagulant solution for hemolymph
collection and prophenolaxidase studies of penaeid shrimp (Penaeus
californiensis). Comp. Biochem. Physiol., 1993 (106A): 299-303.
Vargas-Albores F, Guzmán M A and Ochoa J L. A lipopolysaccharide-binding
agglutinin isolated from brown shrimp (Penaeus californiensis Holmes)
haemolymph. Comparative Biochemistry and Physiology, 1993a(104B):
407-413.
Vargas-Albores F, Hinojosa-Baltazar P, Portillo-Clark G, Magallon-Barajas F.
Influence of temperature and salinity on the yellowleg shrimp, Penaeus
californiensis Holmes, prophenoloxidase system. Aquacult. Res., 1998(29):
549-553.
Vargas-Albores F, Monta?o-Pérez K, Reyes-Izquierdo T, Higuera-Ciapara I and
Yepiz-Plascencia G. The shrimp coagulation system. Developmental &
Comparative Immunology, 1997 (21): 213.
Vargas-Albores F, Yepiz-Plascencia G. Beta glucan binding protein and its role in
shrimp immune response. Aquaculture, 2000(191): 13-21.
114
对虾白斑综合症抗病力指标的筛选及其应用的研究
Vethaak A D and Rheinallt T. Fish disease as a monitor for marine pollution: the case
of the North Sea. Rev. Fish Biol. Fish., 1992 (2): 1-32.
Vici V, Singh I S B, Bhat S G. Application of bacterins and yeast Acremonium
dyosporii to protect the larvae of Macrobrachium rosenbergii from vibriosis.
Fish Shellfish Immunol., 2000(10): 559-563.
Vogan C L, Rowley A F. Effects of shell disease syndrome on the haemocytes and
humoral defences of the edible crab, Cancer pagurus. Aquaculture, 2002(205):
237-252.
Volety A K, Chu F L E. Suppression of chemiluminescence of eastern oyster
Crassostrea ?irginica hemocytes by the protozoan parasite Perkinsus marinus.
Dev. Comp. Immunol., 1995(19): 135–142.
Wang T, Ward M, Grabowski P S, Secombes C J, Molecular cloning, gene
organization and expression of rainbow trout inducible nitric oxide synthase
(iNOS) gene. EMBL accession No. AJ 295231: 2000.
Wang C H, Lo C F, Leu J H, Chou C M, Yeh P Y, Chou H Y, Tung M C, Chang C F,
Su M S, Kou G H. Purification and genomic analysis of baculovirus associated
with white spot syndrome WSBV of Penaeus monodon. Dis. Aquat. Org.,
1995(23): 239–242.
Weeks-Perkins B A, Chansue N, Wong-Verelle D. Assay of immune function in shrimp
phagcytes: Techniques used as indicators of pesticide exposure. In: Stolen, J.S.,
Fletcher, T.C.,Smith, S.A.,Zelikoff, J.T., Kaattari, S.L., Anderson, R. S.,
S?derh?ll, K., Weekss-Perkins, B. A. (Ed.), Techniques in fish Immunology-4.
SOS Publications, Fair Haven, N J, USA, 1995. pp.223-231.
Weiske J and Wiesner A. Stimulation of NO Synthase Activity in the
Immune-Competent Lepidopteran Estigmene acraea Hemocyte Line. NITRIC
OXIDE: Biology and Chemistry, 1999(3): 123-131.
Wilcox D E and Smith R P. Detection and quantification of nitric oxide using electron
magnetic resonance spectroscopy. Methods: a companion to methods in
enzymology, 1995(7): 59-70.
Wink D A and Ford P C. Nitric oxide reactions important to biological systems: a
115
参考文献
survey of some kinetics investigations. Methods: a companion to methods in
enzymology, 1995(7): 14-20.
Wongprasert K, Khanobdee K, Glunukarn S S, Meeratana P, Withyachumnarnkul B.
Time-course and levels of apoptosis in various tissues of black tiger shrimp
Penaeus monodon infected with white-spot syndrome virus. Dis. Aquat. Org.,
2003(55): 3-10.
Yeh M S, Chen Y L, Tsai I H. The hemolymph clottable protein of tiger shrimp
Penaeus monodon, and related species. Comp. Biochem. Phsiol., 1998(121b):
169-176.
Yepiz-Plascencia G, Jimenez-Vega F, Romo-Figueroa M G, Sotelo-Mundo R R,
Vargas-Albores F. Molecular characterization of the bifunctional VHDL-CP
from the hemolymph of white shrimp Penaeus vannamei.Comparative
Biochemistry and Physiology , 2002(132 B): 585–592.
Yuda M, Hirai M, Hirai K, et al. cDNA cloning, expression and characterization of
nitric oxide synthase from the salivary glands of the blood-sucking insect
Rhodnius prolixus. Eur. J. Biochem., 1996(242): 807–8
Zhang X B, Huang C H and Qin Q W. Antiviral properties of hemocyanin isolated
from shrimp Penaeus monodon. Antiviral Research, 2004(61): 93-99.
陈昌生,黄标,叶兆弘、纪得华等.南美白对虾摄食、生长及存活与温度的关系.
集美大学学报(自然科学版),2001,6(4),296-300.
陈皓文,孙丕喜,宋庆云.外源凝集素—水产动物御敌的有利武器. 黄渤海海洋,
1995(13): 61-70.
樊廷俊, 汪小锋. 中国对虾(Penaeus chinensis)酚氧化酶的分离纯化及其部分
生物化学性质. 生物化学与生物物理学报, 2002(34): 589-594.
何建国,周化民,姚伯等. 白斑综合症杆状病毒的传染途径和宿主种类. 中山大
学学报(自然版),1999(38): 65-69.
黄倢,宋晓玲,于佳,杨丛海.杆状病毒性的皮下造血组织坏死—对虾暴发性流
行病的病原和病理学.海洋水产研究, 1995(16):1-7.
116
对虾白斑综合症抗病力指标的筛选及其应用的研究
江晓路,牟海津.对虾免疫系统及免疫机理的研究方法: 管华诗,海水养殖动物
的免疫、细胞培养和病害研究.山东.山东科学技术出版社. 1999:1-31.
李光友,王青.中国对虾血细胞及其免疫研究. 海洋与湖沼, 1995(26): 591-597.
李光友. 中国对虾疾病与免疫机制.海洋科学,1995(4): 1-3.
李才文,管越强,俞志明. 盐度变化对日本虾暴发白斑综合症病毒病的影响. 海
洋环境科学,2002(21):6-9,33.
林林,丁美丽,孙舰军,朱谨钊,李光友. 有机污染提高对虾对病原菌的易感性
研究.海洋学报,1998(20):90-93.
刘晓云,张志峰,马洪明.中国对虾血细胞酶细胞化学的初步研究. 青岛海洋大
学学报, 2002(32):259-265.
孟凡伦,张玉臻,孔健,马桂荣. 甲壳动物中的酚氧化酶原激活系统研究评价.
海洋与湖沼,1999(30):110-115.
史成银,宋晓玲,黄倢,杨丛海.核酸斑点杂交分析方法检测对虾皮下及造血组
织坏死杆状病毒(HHNBV). 海洋与湖沼,1999(30):486-490.
孙 舰 军 , 丁 美 丽 . 氨 氮 对 中 国 对 虾 抗 病 力 的 影 响 . 海 洋 与 湖
沼,1999(30):267-271.
王宝杰, 王雷.中国对虾血细胞吞噬活动中超氧阴离子(O2 )的产生. 中国水产
?–
科学, 2003(10): 14-18.
王鑫,马桂荣,郑宝灿等. SL—益生素对小白鼠体重及其单核吞噬细胞功能的影
响. 微生物学报, 1995(35): 455-459.
叶燕玲,陈宽智.中国对虾(Penaeus chinensis)血细胞超微结构、分类及计数. 青
岛海洋大学学报, 1993(23): 35-41.
朱忠勇. 实用医学检验学. 北京. 人民军医出版社,1997: 368-378.
姜国建,于仁诚,王云峰,颜天,周名江. 对虾血细胞中一氧化氮合成酶鉴定与
分析方法研究.中国水产科学,2004(11):177-184.
姜国建,于仁诚,王云峰,颜天,周名江.中国明对虾血细胞中一氧化氮合成酶
的鉴定及其在白斑综合症病毒感染过程中的变化. 海洋与湖沼,2004(35):
346-352.
117
对虾白斑综合症抗病力指标的筛选及其应用的研究
文章的完成和发表情况
1)姜国建,于仁诚,王云峰,颜天,周名江. 对虾血细胞中一氧化氮合成酶鉴
定与分析方法研究.中国水产科学,2004(11):177-184.
2)姜国建,于仁诚,王云峰,颜天,周名江.中国明对虾血细胞中一氧化氮合成
酶的鉴定及其在白斑综合症病毒感染过程中的变化. 海洋与湖沼,2004(35):
346-352.
3) Jiang Guanjian, Yu Rencheng, Zhou Mingjiang. Modulatory Effects of
Ammonia-N on the Immune System of Penaeus japonicus to Virulence of White
Spot Syndrome Virus (WSSV).Disease of aquatic organisms (in review).
4) Jiang Guojian, Yu Rencheng, Zhou Mingjiang. Comparative Studies of Nitric
Oxide Synthase in shrimps Fenneropenaeus chinensis and Marsupenaeus
japonicus against White Spot Syndrome Virus Infection. Fish and shellfish
immunology (in review).
5) Yu Rencheng, Jiang Guojian, Li Jun, Wangyun Feng, Zhou Mingjiang.
Comparison of Disease Resistance Ability to White Spot Syndrome Virus
(WSSV) Between Fenneropenaeus chinensis and Penaeus japonicus: An
Immunological Approach. (submitted to Aquaculture).
6) Jiang Guojian, Yu Rencheng, Zhou Mingjiang. A try to understand the roles of
phytoplankton and bacteria in white spot syndrome disease. (Complete)
7) 姜国建,于仁诚.活性氮中间体作为抗病力因子的研究进展.投稿“海洋科
学”.