肝郁血瘀型宫环出血病病症结合大鼠模型的建立及研究
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摘要
目的：本研究在中医理论指导下,探讨肝郁血瘀型宫环出血病证动物模型的建立方法,并在此基础上进行发病机理的研究。
方法：将大鼠随机分为4组：空白组、置环组、模型组、治疗组。通过长期慢性束缚应激结合孤养建立起应激大鼠的肝郁症模型,观察分析在模型建立过程中实验动物的体重、蔗糖水摄入量以及行为学指标方面的变化,进行分析,寻找合适的动物模型建立方法。本研究应用一种全新的蛋白组学技术——抗体芯片技术测量动物模型子宫组织细胞因子水平。分析体重、蔗糖摄入量、行为与肝郁症的相关关系,探讨细胞因子在肝郁血瘀型宫环出血病病理生理机制中的作用,观测治疗药物对这些指标的影响以佐证模型。
结果：模型组大鼠行为异常,主要表现为躲避、活动减少、大便松散、皮色失去光泽,生长较对照组缓慢(P<0.05)。糖水偏好实验显示造模过程中模型组对糖水的偏好摄入少于对照组(P<0.05)。与置环组相比,肝郁血瘀+置环模型组有4种因子的水平发生了显著变化。其中睫状神经营养因子(CNTF)水平同置环组相比降低(P<0.05),白介素-1β(IL-1β)、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平较置环组显著升高(P<0.05)。
结论：金刃损伤胞宫是宫环出血关键病因,肝气郁结,瘀血阻络为其主要发病机理。本试验从病因学、行为学和病理表现上用慢性束缚的方法成功构建了肝郁证大鼠模型。研究提示,抗体芯片技术能够高通量、平行性检测大鼠子宫多种细胞因子的表达水平,CNTF、IL-1β、IL-6、TNF-α可能与肝郁症发病机制有关。抗体芯片技术有可能成为中医症候学研究的新型技术平台。
objective:This topic was guided by traditional Chinese medicine to search for a method of building up the animal model with liver depression and blood stasis syndrome of GongHuanChuXue(GHCX:endometrorrhagia which is caused by IUD-wearing) diseases and TCM syndromes.Moreover, to study the pathogenesis of GHCX on the model.
Methods:The rats were divided into normal control group, only IUD placement group, model group and treatment group. Liver depression is a multifaceted psychological disorder that involves changes in behavior, immunological function and responses. Make rat Liver depression model by chronic stress on restrictions on the activities and isolate fed.Observe the weight, sucrose preference solution and automatic behavior. Analyze all of the data from experiment. In the present study, we applied a novel proteomic technology, antibody array, to measure the level of uterus cytokines of the rat model. Observe the correlation between weight, sucrose preference and behavior. Discuss the role of cytokines playing in the liver depression and through treatment confirmed the Cu-IUD liver depression rat model is reasonable.
Results:There were abnormal behaviors, such as decrease of elusion, slackness, looser stools, and mattefur were observed among model group rats. The body weight of model group was signifi cantly lower than that of control group (P<0.05). Fluid consumption test revealed that model rats lost appetite for sweet water (P<0.05) during the whole procession.comparing with only IUD-wearing group, a total of 4 cytokines in uterus from liver depression and blood stasis with Cu-IUD-wearing group was varied significantly.It was shown that the level of CINC declined significantly compared with the IUD-wearing group, and the level of IL-1β、IL-6、TNF-aincreased (P<0.05).
Conclusion:The nature of GHCX's pathogenesis was the impairment by the metal foreign material directly and the main pathogenesis of GHCX was the stagnation of blood in vessel and the syndrome of stagnation of liver qi.This experiment based on etiological, behavioral and pathological observation, it can be concluded that the rat model of syndrome of stagnation of liver qi was established successfully by restrictions on the activities and isolate fed. This studies has shown that antibody array could serve as a fast, high-throughput and sensitive tool for detecting levels of rat's uterus cytokines, and CNTF、IL-1β、IL-6、TNF-αare possibly related with the pathophysiology of syndrome of stagnation of liver qi.
方法：将大鼠随机分为4组：空白组、置环组、模型组、治疗组。通过长期慢性束缚应激结合孤养建立起应激大鼠的肝郁症模型,观察分析在模型建立过程中实验动物的体重、蔗糖水摄入量以及行为学指标方面的变化,进行分析,寻找合适的动物模型建立方法。本研究应用一种全新的蛋白组学技术——抗体芯片技术测量动物模型子宫组织细胞因子水平。分析体重、蔗糖摄入量、行为与肝郁症的相关关系,探讨细胞因子在肝郁血瘀型宫环出血病病理生理机制中的作用,观测治疗药物对这些指标的影响以佐证模型。
结果：模型组大鼠行为异常,主要表现为躲避、活动减少、大便松散、皮色失去光泽,生长较对照组缓慢(P<0.05)。糖水偏好实验显示造模过程中模型组对糖水的偏好摄入少于对照组(P<0.05)。与置环组相比,肝郁血瘀+置环模型组有4种因子的水平发生了显著变化。其中睫状神经营养因子(CNTF)水平同置环组相比降低(P<0.05),白介素-1β(IL-1β)、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平较置环组显著升高(P<0.05)。
结论：金刃损伤胞宫是宫环出血关键病因,肝气郁结,瘀血阻络为其主要发病机理。本试验从病因学、行为学和病理表现上用慢性束缚的方法成功构建了肝郁证大鼠模型。研究提示,抗体芯片技术能够高通量、平行性检测大鼠子宫多种细胞因子的表达水平,CNTF、IL-1β、IL-6、TNF-α可能与肝郁症发病机制有关。抗体芯片技术有可能成为中医症候学研究的新型技术平台。
objective:This topic was guided by traditional Chinese medicine to search for a method of building up the animal model with liver depression and blood stasis syndrome of GongHuanChuXue(GHCX:endometrorrhagia which is caused by IUD-wearing) diseases and TCM syndromes.Moreover, to study the pathogenesis of GHCX on the model.
Methods:The rats were divided into normal control group, only IUD placement group, model group and treatment group. Liver depression is a multifaceted psychological disorder that involves changes in behavior, immunological function and responses. Make rat Liver depression model by chronic stress on restrictions on the activities and isolate fed.Observe the weight, sucrose preference solution and automatic behavior. Analyze all of the data from experiment. In the present study, we applied a novel proteomic technology, antibody array, to measure the level of uterus cytokines of the rat model. Observe the correlation between weight, sucrose preference and behavior. Discuss the role of cytokines playing in the liver depression and through treatment confirmed the Cu-IUD liver depression rat model is reasonable.
Results:There were abnormal behaviors, such as decrease of elusion, slackness, looser stools, and mattefur were observed among model group rats. The body weight of model group was signifi cantly lower than that of control group (P<0.05). Fluid consumption test revealed that model rats lost appetite for sweet water (P<0.05) during the whole procession.comparing with only IUD-wearing group, a total of 4 cytokines in uterus from liver depression and blood stasis with Cu-IUD-wearing group was varied significantly.It was shown that the level of CINC declined significantly compared with the IUD-wearing group, and the level of IL-1β、IL-6、TNF-aincreased (P<0.05).
Conclusion:The nature of GHCX's pathogenesis was the impairment by the metal foreign material directly and the main pathogenesis of GHCX was the stagnation of blood in vessel and the syndrome of stagnation of liver qi.This experiment based on etiological, behavioral and pathological observation, it can be concluded that the rat model of syndrome of stagnation of liver qi was established successfully by restrictions on the activities and isolate fed. This studies has shown that antibody array could serve as a fast, high-throughput and sensitive tool for detecting levels of rat's uterus cytokines, and CNTF、IL-1β、IL-6、TNF-αare possibly related with the pathophysiology of syndrome of stagnation of liver qi.
引文
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