拟南芥MAPKKKX参与ABA调控主根生长分子机制初探
摘要
为了适应复杂多变的外部环境，植物形成了多种多样的信号转导途径，从而使细胞能够做出适当的反应。在这些机制中，其中丝裂原活化蛋白激酶（MAPK）级联途径普遍存在于真核生物中。丝裂原活化蛋白激酶途径由三种信号转导分子组成，丝裂原活化蛋白激酶激酶激酶（MAPKKK），丝裂原活化蛋白激酶激酶（MAPKK），丝裂原活化蛋白激酶（MAPK）。当细胞在应答刺激时，MAPKKK通过磷酸化作用激活MAPKK，后者则通过磷酸化激活MAPK。拟南芥基因组编码了60-80个MAPKKK，10个MAPKK，20个MAPK。已经有报道称MAPK级联途径参与了ABA信号转导过程，而对其是否参与ABA抑制主根生长仍不清楚。
本文鉴定并获得拟南芥中MAPKKK家族成员之一的T-DNA插入纯合突变体mapkkkx，并利用该遗传材料对MAPKKKX在ABA调控的主根生长过程中的作用进行分析。表型分析发现，与野生型相比，在不存在外源ABA时，突变体mapkkkx在主根伸长方面与野生型没有差别，而在50μMABA处理的情况下，突变体的主根伸长是野生型的2倍多。因此我们认为突变体mapkkkx在主根伸长方面对ABA不敏感。但是其他激素和胁迫的处理没有显示出这种差别。RT-PCR的结果显示ABA可抑制MAPKKKX的转录，且MAPKKKX调节了ABA相关基因的表达。为了确定该突变体的表型是由MAPKKKX引起的，我们构建了回补载体和超表达载体。瞬转GFP的结果证明MAPKKKX定位于细胞质中。酵母双杂实验的结果显示MAPKKKX能够与MKK1-MKK10发生相互作用。为了证实酵母双杂的结果，我们构建了双分子荧光互补的一系列载体。
以上的结果表明MAPKKKX参与了ABA调控的主根生长过程。
Plants have developed various signal transduction pathways in order to modulate diverse cellularresponses for adaptation to changing environment conditions. Among these machineries, mitogen-activatedprotein kinase (MAPK) cascades are fundamental signal pathways conserved in eukaryotes.Mitogen-activated protein kinase cascades consist of three signal transducing components, MAPKKKs,MAPKKs and MAPKs. In response to stimuli, MAPKKK can activate MAPKK by phosphorylation, whichin turn activates MAPK by phosphorylation. The Arabidopsis (Arabidopsis thaliana) genome encodes60-80predicted MAPKKKs,10MAPKKs and20MAPKs. It has been reported that MAPK cascades havean important role in ABA signal transduction, however, it is not clear whether MAPK cascades involved inthe primary root growth modulated by ABA in Arabidopsis.
We identified T-DNA insertion homozygous mutant mapkkkx of MAPKKK family in Arabidopsis. Wetherefore used it to analyze the function of MAPKKKX in primary root growth modulated by ABA inArabidopsis. By phenotype analysis, in the absence of ABA, primary root growth was indistinguishablebetween mapkkkx and WT. However, the primary roots of mapkkkx were more than twice as long as theprimary roots of WT on the50μM ABA plate. We concluded that mapkkkx showed insensitive to ABA inprimary root length in contrast to WT. However, other phytohormones and stress could not provide thedifference. RT-PCR revealed ABA repressed the transcription of MAPKKKX, and MAPKKKX regulatedthe expression of marker genes responding to ABA. In order to verify that MAPKKKX brought thephenotype of the mutant, we constructed the MAPKKKX complementary vector and overexpression vector.Fluorescence localization result of MAPKKKX::GFP showed MAPKKKX localized in cytoplasm. Theresults of the yeast two-hybrid revealed that MAPKKKX interacted MKK1-MKK10. In order to verify thevalidity of the yeast two-hybrid, we constructed the vectors of BiFC.
These results suggested that MAPKKK involved in the primary root growth modulated by ABA inArabidopsis.
本文鉴定并获得拟南芥中MAPKKK家族成员之一的T-DNA插入纯合突变体mapkkkx，并利用该遗传材料对MAPKKKX在ABA调控的主根生长过程中的作用进行分析。表型分析发现，与野生型相比，在不存在外源ABA时，突变体mapkkkx在主根伸长方面与野生型没有差别，而在50μMABA处理的情况下，突变体的主根伸长是野生型的2倍多。因此我们认为突变体mapkkkx在主根伸长方面对ABA不敏感。但是其他激素和胁迫的处理没有显示出这种差别。RT-PCR的结果显示ABA可抑制MAPKKKX的转录，且MAPKKKX调节了ABA相关基因的表达。为了确定该突变体的表型是由MAPKKKX引起的，我们构建了回补载体和超表达载体。瞬转GFP的结果证明MAPKKKX定位于细胞质中。酵母双杂实验的结果显示MAPKKKX能够与MKK1-MKK10发生相互作用。为了证实酵母双杂的结果，我们构建了双分子荧光互补的一系列载体。
以上的结果表明MAPKKKX参与了ABA调控的主根生长过程。
Plants have developed various signal transduction pathways in order to modulate diverse cellularresponses for adaptation to changing environment conditions. Among these machineries, mitogen-activatedprotein kinase (MAPK) cascades are fundamental signal pathways conserved in eukaryotes.Mitogen-activated protein kinase cascades consist of three signal transducing components, MAPKKKs,MAPKKs and MAPKs. In response to stimuli, MAPKKK can activate MAPKK by phosphorylation, whichin turn activates MAPK by phosphorylation. The Arabidopsis (Arabidopsis thaliana) genome encodes60-80predicted MAPKKKs,10MAPKKs and20MAPKs. It has been reported that MAPK cascades havean important role in ABA signal transduction, however, it is not clear whether MAPK cascades involved inthe primary root growth modulated by ABA in Arabidopsis.
We identified T-DNA insertion homozygous mutant mapkkkx of MAPKKK family in Arabidopsis. Wetherefore used it to analyze the function of MAPKKKX in primary root growth modulated by ABA inArabidopsis. By phenotype analysis, in the absence of ABA, primary root growth was indistinguishablebetween mapkkkx and WT. However, the primary roots of mapkkkx were more than twice as long as theprimary roots of WT on the50μM ABA plate. We concluded that mapkkkx showed insensitive to ABA inprimary root length in contrast to WT. However, other phytohormones and stress could not provide thedifference. RT-PCR revealed ABA repressed the transcription of MAPKKKX, and MAPKKKX regulatedthe expression of marker genes responding to ABA. In order to verify that MAPKKKX brought thephenotype of the mutant, we constructed the MAPKKKX complementary vector and overexpression vector.Fluorescence localization result of MAPKKKX::GFP showed MAPKKKX localized in cytoplasm. Theresults of the yeast two-hybrid revealed that MAPKKKX interacted MKK1-MKK10. In order to verify thevalidity of the yeast two-hybrid, we constructed the vectors of BiFC.
These results suggested that MAPKKK involved in the primary root growth modulated by ABA inArabidopsis.
引文
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