解淀粉芽孢杆菌抗菌脂肽bacillomycin L的纯化鉴定及抑菌机理研究
|
摘要
本研究以从柠檬样品中筛选分离到一株能够抗立枯丝核菌的解淀粉芽孢杆菌K103为试材,对其所产脂肽的分子结构、生化特性、抗菌机理及植物生防作用进行深入研究,为该抗菌脂肽的生产与运用提供理论基础与技术支持。所得主要研究结果与结论如下：
(1)通过形态学鉴定、生理生化实验及16S rDNA的分子生物学等方法,对产抗菌脂肽的K103菌株进行了分类学鉴定,最终确定菌株K103为解淀粉芽孢杆菌,并将其命名为解淀粉芽孢杆菌K103(Bacillus amyloliquefaciens K103).
(2)解淀粉芽孢杆菌K103发酵上清液经酸沉醇提所得脂肽粗提物,经SephadexLH-20凝胶层析、制备型C18反相高效液相层析分离纯化后,得到两种具有强烈抑制立枯丝核菌的纯化组分P3、P4。采用氨基酸组成分析结合串联质谱的方法对抗菌纯化组分P3、P4的结构进行分析鉴定,最终确定组分P3、P4分别为含有14个和15个碳原子脂肪酸链的bacillomycin L类脂肽同系物。
(3)特性分析结果显示,bacillomycin L抗菌谱广,稳定性高,对热、酶不敏感,能抗紫外照射,在食品防腐和植物生物防治方面有巨大的应用潜力。
(4)荧光染料实验、电镜实验、人工模拟膜实验结果显示,脂肽bacillomycin L处理立枯丝核菌菌丝体细胞后,细胞表面粗糙,细胞内部原生质团聚、分布不均匀以及胞内物质流失,细胞形态改变、结构破坏,细胞膜通透性和完整性破坏,膜流动性增加。
(5)凝胶阻滞实验和光谱吸收滴定实验表明,bacillomycin L可与立枯丝核菌DNA在体外非特异性结合,说明bacillomycin L可能作用于胞内其它目标物质。
(6)SDS-PAGE电泳分析发现bacillomycin L处理前后各分子量范围未发生条带缺失,但条带变浅；Native-PAGE电泳分析发现,bacillomycinL处理非变性可溶性蛋白条带差异较为明显,出现部分条带缺失、变浅情况。
(7)以未处理立枯丝核菌菌丝体胞内总蛋白2-DE图谱为参考图谱,bacillomycinL处理后,差异倍数大于2倍且有显著性差异的蛋白点共有48个,明显上调的点有43个,明显下调的蛋白点有5个。对这些差异蛋白质点进行了质谱鉴定及功能分析表明,这些蛋白质主要参与糖代谢,核苷酸代谢,氨基酸代谢,跨膜运输,抗逆反应相关蛋白,转录,核苷酸结合和蛋白降解以适应胁迫条件。部分差异表达蛋白的qRT-PCR分析和ATPase活性分析实验,蛋白所对应基因表达的变化和酶活性的变化,与相应差异蛋白质表达的变化趋势基本一致。
(8)通过盆栽试验,研究根际接种bacillomycinL和芽孢杆菌K103对黄瓜幼苗生长、养分吸收利用和立枯病防治的效应研究。施用bacillomycin L(500μg/ml)和芽孢杆菌K103能显著促进黄瓜苗期植株生长,提高植株养分吸收,有效防控立枯病发生。
In order to provided technical support and theoretical basis for the development and application of lipopeptide antibiotics as a biological control agent, the purification, identification, physical and chemical characteristics, mechanism of action along with application in biocontrol of bacillomycin L produced by Bacillus amyloliquefaciens K103, isolated from a lemon sample and suppressing Rhizoctonia solani Kiihn, were investigated in detail.
(1)The organism of interest was isolated from the surface of a lemon sample, identified as a B. amyloliquefaciens strain, and designated as B.amyloliquefaciens K103using biochemical, physiological, and16S ribosomal DNA sequencing.
(2)Two antifungal compounds were purified from the culture broth using acid precipitation, gel permeation chromatography, and reversed-phase high-performance liquid chromatography. Based on its amino acid composition and MS/MS spectrometry analysis, they were successfully identified as bacillomycin L and its derivatives, varying only in terms of the length of the fatty acid aliphatic chains from C14to C15.
(3)Results obtained from charateristics analysis of bacillomycin L shows it has a broad spectrum, high stability to heat, enzyme, and ultra-vioket radiation,which indicated it has a potentail application in biological control and food conservation.
(4)Various fluorescent techniques, electron microscopy and model membrane assay show that the surface of cells was rough with more wrinkles and vesicles, the membranes show deformation and materials within the cytoplasm seem to be shrunken an unevenly dispersed, the fluidity of membrane was increased and the integrity and permeabilization of membrane was disrupted.
(5)Gel retardation experiments and absorption titrations assay showed that bacillomycin L could bind to R. solani hyphal cells DNA in vitro,indicating that bacillomycin L was likely to interaction with the intracellular targets.
(6) The protein bands of R. solani hyphal cells detected by SDS-PAGE were fleeted but not disappeared. Native-PAGE showed that non-denaturation protein bands of R. solani hyphal cells were diffenrent markedly with some bands fleeted and disappeared.
(7)As compared with the untreated sample, there were48spots whose abundence varied significantly (more than2-fold),43spots significantly increased and5spots decreased after treated by bacillomycin L. matrixassisted laser desorption/ionization time-of-flight tandem mass spectrometry(MALDI-TOF/TOF-MS) and function classification analysis indicated these protein were involved in carbohydrate metabolism, nucleotide metabolism, amino acids metabolism, transmembrane transport, stress response,transcription, nucleotide binding, protein degradation, and cellular component organization. Real-time PCR and ATPase activity analysis of the hyphal proteins differentially expressed indicated that these differentially expression were consistented with corresponding gene expression roughly.
(8)A pot experiment was carried out to investigate the effects of bacillomycin L and Bacillus amyloliquefaciens K103on the growth of cucumber seedlings, absorption and utilization of nutrient and the suppression of the seedlings damping-off disease. The results indicated that the application of bacillomycin L and Bacillus amyloliquefaciens K103could effectively promote growth, nutrition absorption of banana seedlings, and reduce the occurrence of seedlings damping-off.
(1)通过形态学鉴定、生理生化实验及16S rDNA的分子生物学等方法,对产抗菌脂肽的K103菌株进行了分类学鉴定,最终确定菌株K103为解淀粉芽孢杆菌,并将其命名为解淀粉芽孢杆菌K103(Bacillus amyloliquefaciens K103).
(2)解淀粉芽孢杆菌K103发酵上清液经酸沉醇提所得脂肽粗提物,经SephadexLH-20凝胶层析、制备型C18反相高效液相层析分离纯化后,得到两种具有强烈抑制立枯丝核菌的纯化组分P3、P4。采用氨基酸组成分析结合串联质谱的方法对抗菌纯化组分P3、P4的结构进行分析鉴定,最终确定组分P3、P4分别为含有14个和15个碳原子脂肪酸链的bacillomycin L类脂肽同系物。
(3)特性分析结果显示,bacillomycin L抗菌谱广,稳定性高,对热、酶不敏感,能抗紫外照射,在食品防腐和植物生物防治方面有巨大的应用潜力。
(4)荧光染料实验、电镜实验、人工模拟膜实验结果显示,脂肽bacillomycin L处理立枯丝核菌菌丝体细胞后,细胞表面粗糙,细胞内部原生质团聚、分布不均匀以及胞内物质流失,细胞形态改变、结构破坏,细胞膜通透性和完整性破坏,膜流动性增加。
(5)凝胶阻滞实验和光谱吸收滴定实验表明,bacillomycin L可与立枯丝核菌DNA在体外非特异性结合,说明bacillomycin L可能作用于胞内其它目标物质。
(6)SDS-PAGE电泳分析发现bacillomycin L处理前后各分子量范围未发生条带缺失,但条带变浅；Native-PAGE电泳分析发现,bacillomycinL处理非变性可溶性蛋白条带差异较为明显,出现部分条带缺失、变浅情况。
(7)以未处理立枯丝核菌菌丝体胞内总蛋白2-DE图谱为参考图谱,bacillomycinL处理后,差异倍数大于2倍且有显著性差异的蛋白点共有48个,明显上调的点有43个,明显下调的蛋白点有5个。对这些差异蛋白质点进行了质谱鉴定及功能分析表明,这些蛋白质主要参与糖代谢,核苷酸代谢,氨基酸代谢,跨膜运输,抗逆反应相关蛋白,转录,核苷酸结合和蛋白降解以适应胁迫条件。部分差异表达蛋白的qRT-PCR分析和ATPase活性分析实验,蛋白所对应基因表达的变化和酶活性的变化,与相应差异蛋白质表达的变化趋势基本一致。
(8)通过盆栽试验,研究根际接种bacillomycinL和芽孢杆菌K103对黄瓜幼苗生长、养分吸收利用和立枯病防治的效应研究。施用bacillomycin L(500μg/ml)和芽孢杆菌K103能显著促进黄瓜苗期植株生长,提高植株养分吸收,有效防控立枯病发生。
In order to provided technical support and theoretical basis for the development and application of lipopeptide antibiotics as a biological control agent, the purification, identification, physical and chemical characteristics, mechanism of action along with application in biocontrol of bacillomycin L produced by Bacillus amyloliquefaciens K103, isolated from a lemon sample and suppressing Rhizoctonia solani Kiihn, were investigated in detail.
(1)The organism of interest was isolated from the surface of a lemon sample, identified as a B. amyloliquefaciens strain, and designated as B.amyloliquefaciens K103using biochemical, physiological, and16S ribosomal DNA sequencing.
(2)Two antifungal compounds were purified from the culture broth using acid precipitation, gel permeation chromatography, and reversed-phase high-performance liquid chromatography. Based on its amino acid composition and MS/MS spectrometry analysis, they were successfully identified as bacillomycin L and its derivatives, varying only in terms of the length of the fatty acid aliphatic chains from C14to C15.
(3)Results obtained from charateristics analysis of bacillomycin L shows it has a broad spectrum, high stability to heat, enzyme, and ultra-vioket radiation,which indicated it has a potentail application in biological control and food conservation.
(4)Various fluorescent techniques, electron microscopy and model membrane assay show that the surface of cells was rough with more wrinkles and vesicles, the membranes show deformation and materials within the cytoplasm seem to be shrunken an unevenly dispersed, the fluidity of membrane was increased and the integrity and permeabilization of membrane was disrupted.
(5)Gel retardation experiments and absorption titrations assay showed that bacillomycin L could bind to R. solani hyphal cells DNA in vitro,indicating that bacillomycin L was likely to interaction with the intracellular targets.
(6) The protein bands of R. solani hyphal cells detected by SDS-PAGE were fleeted but not disappeared. Native-PAGE showed that non-denaturation protein bands of R. solani hyphal cells were diffenrent markedly with some bands fleeted and disappeared.
(7)As compared with the untreated sample, there were48spots whose abundence varied significantly (more than2-fold),43spots significantly increased and5spots decreased after treated by bacillomycin L. matrixassisted laser desorption/ionization time-of-flight tandem mass spectrometry(MALDI-TOF/TOF-MS) and function classification analysis indicated these protein were involved in carbohydrate metabolism, nucleotide metabolism, amino acids metabolism, transmembrane transport, stress response,transcription, nucleotide binding, protein degradation, and cellular component organization. Real-time PCR and ATPase activity analysis of the hyphal proteins differentially expressed indicated that these differentially expression were consistented with corresponding gene expression roughly.
(8)A pot experiment was carried out to investigate the effects of bacillomycin L and Bacillus amyloliquefaciens K103on the growth of cucumber seedlings, absorption and utilization of nutrient and the suppression of the seedlings damping-off disease. The results indicated that the application of bacillomycin L and Bacillus amyloliquefaciens K103could effectively promote growth, nutrition absorption of banana seedlings, and reduce the occurrence of seedlings damping-off.
引文
Alvarez, F., Castro, M., Principe, A., Borioli, G., Fischer, S., Mori, G, Jofre, E.2012. The plant-associated Bacillus amyloliquefaciens strains MEP218 and ARP23 capable of producing the cyclic lipopeptides iturin or surfactin and fengycin are effective in biocontrol of sclerotinia stem rot disease. Journal ofApplied Microbiology,112(1),159-174.
Aranda, F.J., Teruel, J.A., Ortiz, A.2005. Further aspects on the hemolytic activity of the antibiotic lipopeptide iturin A. Biochimica et Biophysica Acta (BBA)-Biomembranes,1713(1),51-56.
Arrebola, E., Jacobs, R., Korsten, L.2010. Iturin A is the principal inhibitor in the biocontrol activity of Bacillus amyloliquefaciens PPCB004 against postharvest fungal pathogens. Journal of Applied Microbiology,108(2),386-395.
Asaka, O., Shoda, M.1996. Biocontrol of Rhizoctonia solani damping-off of tomato with Bacillus subtilis RB14. Applied and Environmental Microbiology,62(11),4081-4085.
Bais, H.P., Fall, R., Vivanco, J.M.2004. Biocontrol of Bacillus subtilis against infection of Arabidopsis roots by Pseudomonas syringae is facilitated by biofilm formation and surfactin production. Plant Physiology,134(1),307-319.
Batut, J., Howell, M., Hill, C.S.2007. Kinesin-mediated transport of Smad2 is required for signaling in response to TGF-β ligands. Developmental Cell,12(2),261-274.
Baysal, O., Caliskan, M., Yesilova, O.2008. An inhibitory effect of a new< Bacillus subtilis strain (EU07) against Fusarium oxysporum f. sp. radicis-lycopersici. Physiological and Molecular Plant Pathology,73(1),25-32.
Bedford, L., Paine, S., Sheppard, P.W., Mayer, R.J., Roelofs, J.2010. Assembly, structure, and function of the 26S proteasome. Trends in Cell Biology,20(7),391-401.
Besson, F., Peypoux, F., Michel, G., Delcambe, L.1979. Antifungal activity upon Saccharomyces cerevisiae of iturin A, mycosubtilin, bacillomycin L and of their derivatives; inhibition of this antifungal activity by lipid antagonists. The Journal of antibiotics,32(8),828.
Bie, X., Lu, Z., Lu, F.2009. Identification of fengycin homologues from Bacillus subtilis with ESI-MS/CID. Journal of Microbiological Methods,79(3),272-278.
Binder, U., Bencina, M., Eigentler, A., Meyer, V, Marx, F.2011. The Aspergillus giganteus antifungal protein AFPNN5353 activates the cell wall integrity pathway and perturbs calcium homeostasis. BMC Microbiol,11,209.
Binder, U., Chu, M., Read, N.D., Marx, F.2010. The antifungal activity of the Penicillium chrysogenum protein PAF disrupts calcium homeostasis in Neurospora crassa. Eukaryot Cell,9(9),1374-82.
Brogden, K.A.2005. Antimicrobial peptides:pore formers or metabolic inhibitors in bacteria? Nat Rev Microbiol,3(3),238-50.
Bruni, R., Gianfranceschi, G., Koch, G 2004. On peptide de novo sequencing:a new approach. Journal of Peptide Science,11(4),225-234.
Candiano, G, Bruschi, M., Musante, L., Santucci, L., Ghiggeri, G.M., Carnemolla, B., Orecchia, P., Zardi, L., Righetti, P.G 2004. Blue silver:a very sensitive colloidal Coomassie G-250 staining for proteome analysis. Electrophoresis,25(9),1327-33.
Cao, Y., Zhang, Z., Ling, N., Yuan, Y., Zheng, X., Shen, B., Shen, Q.2011. Bacillus subtilis SQR 9 can control Fusarium wilt in cucumber by colonizing plant roots. Biology and Fertility of Soils,47(5), 495-506.
Carrillo, C., Teruel, J.A., Aranda, F.J., Ortiz, A.2003. Molecular mechanism of membrane permeabilization by the peptide antibiotic surfactin. Biochimica et Biophysica Acta (BBA)-Biomcmbranes,1611(1),91-97.
Catala, R., Santos, E., Alonso, J.M., Ecker, J.R., Martinez-Zapater, J.M., Salinas, J.2003. Mutations in the Ca2+/H+ transporter CAX1 increase CBF/DREB1 expression and the cold-acclimation response in Arabidopsis. Plant Cell,15(12),2940-51.
Chen, H., Wang, L., Su, C., Gong, G., Wang, P., Yu, Z.2008. Isolation and characterization of lipopeptide antibiotics produced by Bacillus subtilis. Letters in applied microbiology,47(3),180-186.
Chen, X.H., Koumoutsi, A., Scholz, R., Eisenreich, A., Schneider, K., Heinemeyer, I., Morgenstern, B., Voss, B., Hess, W.R., Reva, O., Junge, H., Voigt, B., Jungblut, P.R., Vater, J., Sussmuth, R., Liesegang, H., Strittmatter, A., Gottschalk, G., Borriss, R.2007. Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42. Nature Biotechnology,25(9),1007-14.
Cheng, S., McCleskey, F.K., Gress, M.J., Petroziello, J.M., Liu, R., Namdari, H., Beninga, K., Salmen, A., DelVecchio, V.G.1997. A PCR assay for identification of Enterococcus faecium. Journal of clinical microbiology,35(5),1248-1250.
Chiapello, M., Daghino, S., Martino, E., Perotto, S.2010. Cellular response of Fusarium oxysporum to crocidolite asbestos as revealed by a combined proteomic approach. Journal of Proteome research, 9(8),3923-3931.
Chung, S., Kong, H., Buyer, J.S., Lakshman, D.K., Lydon, J., Kim, S.-D., Roberts, D.P.2008. Isolation and partial characterization of Bacillus subtilis ME488 for suppression of soilborne pathogens of cucumber and pepper. Applied Microbiology and Biotechnology,80(1),115-123.
Deleu, M., Paquot, M., Nylander, T.2008. Effect of Fengycin, a Lipopeptide Produced by Bacillus subtilis, on Model Biomembranes. Biophysical Journal,94(7),2667-2679.
Deleu, M., Paquot, M., Nylander, T.2005. Fengycin interaction with lipid monolayers at the air-aqueous interface-implications for the effect of fengycin on biological membranes. Journal of Colloid and Interface Science,283(2),358-365.
Even, S., Lindley, N.D., Loubiere, P., Cocaign-Bousquet, M.2002. Dynamic response of catabolic pathways to autoacidification in Lactococcus lactis:transcript profiling and stability in relation to metabolic and energetic constraints. Molecular Microbiology,45(4),1143-1152.
Fang, X., Jost, R., Finnegan, P.M., Barbetti, M.J.2013. Comparative Proteome Analysis of the Strawberry-Fusarium oxysporum f. sp. fragariae Pathosystem Reveals Early Activation of Defense Responses as a Crucial Determinant of Host Resistance. Journal of Proteome research, 12(4),1772-1788.
Fernandez-Acero, F.J., Jorge, I., Calvo, E., Vallejo, I., Carbu, M., Camafeita, E., Lopez, J.A., Cantoral, J.M., Jorrin, J.2006. Two-dimensional electrophoresis protein profile of the phytopathogenic fungus Botrytis cinerea. Proteomics,6 Suppl 1, S88-96.
Gao, Q., Liu, Y., Wang, M., Zhang, J., Gai, Y, Zhu, C., Guo, X.2009. Molecular cloning and characterization of an inducible RNA-dependent RNA polymerase gene, GhRdRP, from cotton (Gossypium hirsutum L.). Molecular Biology Reports,36(1),47-56.
Gautam, P., Shankar, J., Madan, T., Sirdeshmukh, R., Sundaram, C.S., Gade, W.N., Basir, S.F., Sarma, P.U. 2008. Proteomic and transcriptomic analysis of Aspergillus fumigatus on exposure to amphotericin B. Antimicrobial Agents and Chemotherapy,52(12),4220-7.
Gonzalez-Fernandez, R., Prats, E., Jorrin-Novo, J.V.2010. Proteomics of plant pathogenic fungi. BioMed Research International,2010.
Gonzalez-Fernandez, R., Jorrin-Novo, J.V.2011. Contribution of proteomics to the study of plant pathogenic fungi. Journal of Proteome research,11(1),3-16.
Gonzalez, F.J.2005. Role of cytochromes P450 in chemical toxicity and oxidative stress:studies with CYP2E1. Mutation Research,569(1-2),101-10.
Grau, A., Ortiz, A., de Godos, A., Gomez-Fernandez, J.C.2000. A biophysical study of the interaction of the lipopeptide antibiotic iturin A with aqueous phospholipid bilayers. Archives of Biochemistry and Biophysics,377(2),315-323.
Heerklotz, H.2008. Interactions of surfactants with lipid membranes. Q. Rev. Biophys,41(3-4),205-264.
Heerklotz, H., Seelig, J.2007. Leakage and lysis of lipid membranes induced by the lipopeptide surfactin. European Biophysics Journal,36(4-5),305-314.
Hirschi, K.D.1999. Expression of Arabidopsis CAX1 in tobacco:altered calcium homeostasis and increased stress sensitivity. The Plant Cell Online,11(11),2113-2122.
Holzapfel, W.H., Haberer, P., Geisen, R., Bjorkroth, J., Schillinger, U.2001. Taxonomy and important features of probiotic microorganisms in food and nutrition. The American journal of clinical nutrition,73(2),365s-373s.
Hubbard, P.A., Shen, A.L., Paschke, R., Kasper, C.B., Kim, J.J.2001. NADPH-cytochrome P450 oxidoreductase. Structural basis for hydride and electron transfer. Journal of Biological Chemistry, 276(31),29163-70.
Hwang, B., Cho, J., Hwang, I., Jin, H.G., Woo, E.R., Lee, D.G.2011. Antifungal activity of lariciresinol derived from Sambucus williamsii and their membrane-active mechanisms in Candida albicans. Biochemical and biophysical research communications,410(3),489-493.
Jiang, D., Zhao, L., Clapham, D.E.2009. Genome-wide RNAi screen identifies Letml as a mitochondrial Ca2+/H+ antiporter. Science,326(5949),144-7.
Jin, J., Zhang, B., Guo, H., Cui, J., Jiang, L., Song, S., Sun, M., Ren, F.2012. Mechanism analysis of acid tolerance response of bifidobacterium longum subsp. longum BBMN 68 by gene expression profile using RNA-sequencing. PLoS One,7(12), e50777.
Jorge, J.A., Polizeli, M.d.L., Thevelein, J.M., Terenzi, H.F.1997. Trehalases and trehalose hydrolysis in fungi. FEMS Microbiology Letters,154(2),165-171.
Kamiya, T., Akahori, T., Ashikari, M., Maeshima, M.2006. Expression of the vacuolar Ca2+/H+ exchanger, OsCAXla, in rice:cell and age specificity of expression, and enhancement by Ca2+. Plant and Cell Physiology,47(1),96-106.
Kim, K.J., Sung, W.S., Suh, B.K., Moon, S.K., Choi, J.S., Kim, J.G., Lee, D.G.2009a. Antifungal activity and mode of action of silver nano-particles on Candida albicans. Biometals,22(2),235-242.
Kim, K.J., Sung, W.S., Suh, B.K., Moon, S.K., Choi, J.S., Kim, J.G., Lee, D.G.2009b. Antifungal activity and mode of action of silver nano-particles on Candida albicans. BioMetals,22(2),235-42.
Kim, Y., Nandakumar, M., Marten, M.R.2007. Proteomics of filamentous fungi. Trends in Biotechnology, 25(9),395-400.
Kloepper, J., Metting Jr, F.1992. Plant growth-promoting rhizobacteria as biological control agents. Soil microbial ecology:applications in agricultural and environmental management.,255-274.
Kloepper, J., Schroth, M., Miller, T.1980. Effects of rhizosphere colonization by plant growth-promoting rhizobacteria on potato plant development and yield. Phytopathology,70(11),1078-1082.
Kusano, T., Yamaguchi, K., Berberich, T., Takahashi, Y.2007. The polyamine spermine rescues Arabidopsis from salinity and drought stresses. Plant Signal Behav,2(4),251-252.
Lakshman, D.K., Natarajan, S.S., Lakshman, S., Garrett, W.M., Dhar, A.K.2008. Optimized protein extraction methods for proteomic analysis of Rhizoctonia solani. Mycologia,100(6),867-875.
Lee, H., Churey, J., Worobo, R.2008. Purification and structural characterization of bacillomycin F produced by a bacterial honey isolate active against Byssochlamys fulva H25. Journal of Applied Microbiology,105(3),663-673.
Lee, J., Bricker, T.M., Lefevre, M., Pinson, S.R., Oard, J.H.2006. Proteomic and genetic approaches to identifying defence-related proteins in rice challenged with the fungal pathogen Rhizoctonia solani. Mol Plant Pathol,7(5),405-16.
Lee, S.C., Kim, S.H., Park, I.H., Chung, S.Y., Choi, Y. L.2007. Isolation and structural analysis of bamylocin A, novel lipopeptide from Bacillus amyloliquefaciens LP03 having antagonistic and crude oil-emulsifying activity. Archives of Microbiology,188(4),307-312.
Leiter, E., Szappanos, H., Oberparleiter, C., Kaiserer, L., Csernoch, L., Pusztahelyi, T., Emri, T., Pocsi, I., Salvenmoser, W., Marx, F.2005. Antifungal protein PAF severely affects the integrity of the plasma membrane of Aspergillus nidulans and induces an apoptosis-like phenotype. Antimicrobial agents and chemotherapy,49(6),2445-2453.
Maget-Dana, R., Peypoux, F.1994. Iturins, a special class of pore-forming lipopeptides:biological and physicochemical properties. Toxicology,87(1),151-174.
Maget-Dana, R., Thimon, L., Peypoux, F., Ptak, M.1992. Surfactin/iturin A interactions may explain the synergistic effect of surfactin on the biological properties of iturin A. Biochimie,74(12), 1047-1051.
Mahenthiralingam, E., Simpson, D.A., Speert, D.P.1997. Identification and characterization of a novel DNA marker associated with epidemic Burkholderia cepacia strains recovered from patients with cystic fibrosis. Journal of Clinical Microbiology,35(4),808-816.
Mei, H., Zhao, J., Pittman, J.K., Lachmansingh, J., Park, S., Hirschi, K.D.2007. In planta regulation of the Arabidopsis Ca(2+)/H(+) antiporter CAX1. J Exp Bot,58(12),3419-27.
Miki, H., Okada, Y, Hirokawa, N.2005. Analysis of the kinesin superfamily:insights into structure and function. Trends in Cell Biology,15(9),467-76.
Misteli, T., Caceres, J.F., Spector, D.L.1997. The dynamics of a pre-mRNA splicing factor in living cells. Nature,387(6632),523-527.
Mohammadipour, M., Mousivand, M., Salehi Jouzani, G., Abbasalizadeh, S.2009. Molecular and biochemical characterization of Iranian surfactin-producing Bacillus subtilis isolates and evaluation of their biocontrol potential against Aspergillus flavus and Colletotrichum gloeosporioides. Canadian Journal of Microbiology,55(4),395-404.
Munoz, A., Gandia, M., Harries, E., Carmona, L., Read, N.D., Marcos, J.F.2013. Understanding the mechanism of action of cell-penetrating antifungal peptides using the rationally designed hexapeptide PAF26 as a model. Fungal Biology Reviews,26(4),146-155.
Munoz, A., Marcos, J.F., Read, N.D.2012. Concentration-dependent mechanisms of cell penetration and killing by the de novo designed antifungal hexapeptide PAF26. Molecular Microbiology,85(1), 89-106.
Munoz, A., Lopez-Garcia, B., Marcos, J.F.2006. Studies on the mode of action of the antifungal hexapeptide PAF26. Antimicrobial agents and chemotherapy,50(11),3847-3855.
O'Shaughnessy, E.M., Lyman, C.A., Walsh, T.J.2009. Amphotericin B:Polyene resistance mechanisms. Antimicrobial Drug Resistance,295-305.
Ongena, M., Jacques, P.2008. Bacillus lipopeptides:versatile weapons for plant disease biocontrol. Trends in Microbiology,16(3),115-125.
Ongena, M., Jourdan, E., Adam, A., Paquot, M., Brans, A., Joris, B., Arpigny, J.L., Thonart, P.2007. Surfactin and fengycin lipopeptides of Bacillus subtilis as elicitors of induced systemic resistance in plants. Environmental Microbiology,9(4),1084-1090.
Oppenheimer, D.G., Pollock, M.A., Vacik, J., Szymanski, D.B., Ericson, B., Feldmann, K., Marks, M.D. 1997. Essential role of a kinesin-like protein in Arabidopsis trichome morphogenesis. Proceedings of the National Academy of Sciences,94(12),6261-6266.
Palma-Guerrero, J., Lopez-Jimenez, J., Perez-Berna, A., Huang, I.C., Jansson, H.B., Salinas, J., Villalain, J., Read, N., Lopez-Llorca, L.2010. Membrane fluidity determines sensitivity of filamentous fungi to chitosan. Molecular microbiology,75(4),1021-1032.
Pandey, S.P., Baldwin, I.T.2007. RNA-directed RNA polymerase 1 (RdRl) mediates the resistance of Nicotiana attenuata to herbivore attack in nature. Plant Journal,50(1),40-53.
Park, C.B., Kim, H.S., Kim, S.C.1998. Mechanism of action of the antimicrobial peptide buforin II: buforin Ⅱ kills microorganisms by penetrating the cell membrane and inhibiting cellular functions. Biochemical and biophysical research communications,244(1),253-257.
Parrou, J.L., Jules, M., Beltran, G., Francois, J.2005. Acid trehalase in yeasts and filamentous fungi: localization, regulation and physiological function. FEMS Yeast Res,5(6-7),503-11.
Parveen, M., Hasan, M.K., Takahashi, J., Murata, Y., Kitagawa, E., Kodama, O., Iwahashi, H.2004. Response of Saccharomyces cerevisiae to a monoterpene:evaluation of antifungal potential by DNA microarray analysis. Journal of Antimicrobial Chemotherapy,54(1),46-55.
Pena, L.B., Pasquini, L.A., Tomaro, M.L., Gallego, S.M.2007.20S proteasome and accumulation of oxidized and ubiquitinated proteins in maize leaves subjected to cadmium stress. Phytochemistry, 68(8),1139-46.
Qin, G., Tian, S., Chan, Z., Li, B.2007. Crucial role of antioxidant proteins and hydrolytic enzymes in pathogenicity of Penicillium expansum:analysis based on proteomics approach. Mol Cell Proteomics,6(3),425-38.
Raaijmakers, J.M., de Bruijn, I., de Kock, M.J.2006. Cyclic lipopeptide production by plant-associated Pseudomonas spp.:diversity, activity, biosynthesis, and regulation. Molecular plant-microbe interactions,19(7),699-710.
Raaijmakers, J.M., De Bruijn, I., Nybroe, O., Ongena, M.2010. Natural functions of lipopeptides from Bacillus and Pseudomonas:more than surfactants and antibiotics. FEMS Microbiology Reviews, 34(6),1037-1062.
REINHECKEL, T., SITTE, N., ULLRICH, O., KUCKELKORN, U., Davies, K., GRUNE, T.1998. Comparative resistance of the 20S and 26S proteasome to oxidative stress. Biochemical Journal, 335,637-642.
Romero, D., De Vicente, A., Olmos, J., Davila, J., Perez-Garcia, A.2007a. Effect of lipopeptides of antagonistic strains of Bucillus subtilis on the morphology and ultrastructure of the cucurbit fungal pathogen Podosphaera fusca. Journal of Applied Microbiology,103(4),969-976.
Romero, D., de Vicente, A., Rakotoaly, R.H., Dufour, S.E., Veening, J.-W., Arrebola, E., Cazorla, F.M., Kuipers, O.P., Paquot, M., Perez-Garcia, A.2007b. The iturin and fengycin families of lipopeptides are key factors in antagonism of Bacillus subtilis toward Podosphaera fusca. Molecular plant-microbe interactions,20(4),430-440.
Saharan, B., Nehra, V.2011. Plant growth promoting rhizobacteria:a critical review. Life Sci Med Res,21, 1-30.
Schmittgen, T.D., Livak, K.J.2008. Analyzing real-time PCR data by the comparative CT method. Nature protocols,3(6),1101-1108.
Schoch, C.L., Briining, A.R., Entian, K.-D., Pretorius, G.H., Prior, B.A.1997. ASaccharomyces cerevisiae mutant defective in the kinesin-like protein Kar3 is sensitive to NaCl-stress. Current Genetics, 32(5),315-322.
Shen, Q.-T., Chen, X.-L., Sun, C.-Y., Zhang, Y.-Z.2004. Dissecting and exploiting nonribosomal peptide synthetases. Acta biochimica et biophysica Sinica,36(4),243-249.
Shi, C., Zhang, Y., Bian, K., Xu, L.2010. Amount and activity changes of 20S proteasome modified by oxidation in salt-treated wheat root tips.Acta Physiologiae Plantarum,33(4),1227-1237.
Shimizu, M., Fujii, T., Masuo, S., Fujita, K., Takaya, N.2009. Proteomic analysis of Aspergillus nidulans cultured under hypoxic conditions. Proteomics,9(1),7-19.
Sobhanian, H., Razavizadeh, R., Nanjo, Y., Ehsanpour, A.A., Jazii, F.R., Motamed, N., Komatsu, S.2010. Proteome analysis of soybean leaves, hypocotyls and roots under salt stress. Proteome Sci,8,19.
Strieker, M., Tanovic, A., Marahiel, M.A.2010. Nonribosomal peptide synthetases:structures and dynamics. Current Opinion in Structural Biology,20(2),234-240.
te Welscher, Y.M., Hendrik, H., Balague, M.M., Souza, C.M., Riezman, H., De Kruijff, B., Breukink, E. 2008. Natamycin blocks fungal growth by binding specifically to ergosterol without permeabilizing the membrane. Journal of Biological Chemistry,283(10),6393-6401.
Teixeira, M.C., Santos, P.M., Fernandes, A.R., Sa-Correia, I.2005. A proteome analysis of the yeast response to the herbicide 2,4-dichlorophenoxyacetic acid. Proteomics,5(7),1889-901.
Theis, T., Marx, F., Salvenmoser, W., Stahl, U., Meyer, V.2005. New insights into the target site and mode of action of the antifungal protein of Aspergillus giganteus Research in microbiology,156(1), 47-56.
Theis, T., Wedde, M., Meyer, V., Stahl, U.2003. The antifungal protein from Aspergillus giganteus causes membrane permeabilization.Antimicrobial agents and chemotherapy,47(2),588-593.
Thevissen, K., Terras, F.R.G., Broekaert, W.F.1999. Permeabilization of fungal membranes by plant defensins inhibits fungal growth. Applied and environmental microbiology,65(12),5451-5458.
Thimon, L., Peypoux, F., Wallach, J., Michel, G.1995. Effect of the lipopeptide antibiotic, iturin A, on morphology and membrane ultrastructure of yeast cells. FEMS Microbiology Letters,128(2), 101-106.
Topanurak, S., Sinchaikul, S., Phutrakul, S., Sookkheo, B., Chen, S.T.2005. Proteomics viewed on stress response of thermophilic bacterium Bacillus stearothermophilus TLS33. Proteomics,5(14), 3722-30.
Trevors, J.2003. Fluorescent probes for bacterial cytoplasmic membrane research. Journal of biochemical and biophysical methods,57(2),87-103.
van der Weerden, N.L., Hancock, R.E.W., Anderson, M.A.2010. Permeabilization of fungal hyphae by the plant defensin NaDl occurs through a cell wall-dependent process. Journal of Biological Chemistry,285(48),37513-37520.
van der Weerden, N.L., Lay, F.T., Anderson, M.A.2008. The plant defensin, NaDl, enters the cytoplasm of Fusarium oxysporum hyphae. Journal of Biological Chemistry,283(21),14445-14452.
Vater, J., Kablitz, B., Wilde, C., Franke, P., Mehta, N., Cameotra, S.S.2002. Matrix-assisted laser desorption ionization-time of flight mass spectrometry of lipopeptide biosurfactants in whole cells and culture filtrates of Bacillus subtilis C-1 isolated from petroleum sludge. Applied and environmental microbiology,68(12),6210-6219.
Volpon, L., Besson, F., Lancelin, J.M.1999. NMR structure of active and inactive forms of the sterol-dependent antifungal antibiotic bacillomycin L. European Journal of Biochemistry,264(1), 200-210.
Waditee, R., Hossain, G.S., Tanaka, Y., Nakamura, T., Shikata, M., Takano, J., Takabe, T.2004. Isolation and functional characterization of Ca2+/H+ antiporters from cyanobacteria. Journal of Biological Chemistry,279(6),4330-8.
Yan, X., Liang, X., Jin, S., Lv, J., Yu, C., Qi, W., Li, B., Yuan, H., Qi, S., Shi, Y.2010. Primary Study on Mode of Action for Macrocyclic Fungicide Candidates (7B3, D1) against Rhizoctonia solani Kuhn(?). Journal of agricultural and food chemistry,58(5),2726-2729.
Yan, X., Qin, W., Sun, L., Qi, S., Yang, D., Qin, Z., Yuan, H.2009. Study of Inhibitory Effects and Action Mechanism of the Novel Fungicide Pyrimorph against Phytophthora capsici(?). Journal of agricultural and food chemistry,58(5),2720-2725.
Yang, H., Wang, M., Gao, Z., Zhu, C., Guo, X.2011. Isolation of a novel RNA-dependent RNA polymerase 6 from Nicotiana glutinosa, NgRDR6, and analysis of its response to biotic and abiotic stresses. Molecular Biology Reports,38(2),929-37.
Yonezawa, A., Kuwahara, J., Fujii, N., Sugiura, Y.1992. Binding of tachyplesin I to DNA revealed by footprinting analysis:significant contribution of secondary structure to DNA binding and implication for biological action. Biochemistry,31(11),2998-3004.
Yoshida, M., Muneyuki, E., Hisabori, T.2001. ATP synthase-a marvellous rotary engine of the cell. Nature Reviews Molecular Cell Biology,2(9),669-677.
Yu, G, Sinclair, J., Hartman, G., Bertagnolli, B.2002. Production of iturin A by Bacillus amyloliquefaciens suppressing Rhizoctonia solani. Soil Biology and Biochemistry,34(7),955-963.
Yuan, B., Wang, Z., Qin, S., Zhao, G.H., Feng, Y.J., Wei, L.H., Jiang, J. H.2012. Study of the anti-sapstain fungus activity of Bacillus amyloliquefaciens CGMCC 5569 associated with Ginkgo biloba and identification of its active components. Bioresource technology,114(0),536-541.
Zahler, A.M., Lane, W.S., Stolk, J.A., Roth, M.B.1992. SR proteins:a conserved family of pre-mRNA splicing factors. Genes & Development,6(5),837-847.
Zhao, Z., Wang, Q., Wang, K., Brian, K., Liu, C., Gu, Y.2010. Study of the antifungal activity of Bacillus vallismortis ZZ185 in vitro and identification of its antifungal components. Bioresource Technology,101(1),292-297.
王英国,王军华,权春善,范圣第.2007.解淀粉芽孢杆菌抗菌活性物质的分离纯化及抑菌活性研究.中国生物工程杂志,27(12),41-45.
王帅.2009.芽孢杆菌及其脂肽类化合物防治植物病害和促进植物生长的研究,南京：南京农业大学.
林东,徐庆.2001.枯草芽孢杆菌S0113分泌蛋白的抑菌作用及抗菌蛋白的分离纯化.农业生物技 术学报,9(1),77-80.
胡梁斌,周威,章挺,杨志敏,徐剑宏,石志琦.2010.抗菌肽Fengycins抑制串珠镰刀菌的初步机制.微生物学通报,37(2),251-255.
徐杨,王楠,李伟,胡江春,胡芳欣,刘丽,王书锦.2009.海洋枯草芽孢杆菌3512A抗真菌脂肽的分离纯化及结构特性鉴定.中国生物防治,25(4),328-333.
高伟,田黎,周俊英,史振平,郑立,崔志松,李元广.2009.海洋芽孢杆菌(Bacillus marinus) B-9987菌株抑制病原真菌机理.微生物学报(11),1494-1501.
刘静,王军,姚建铭,潘仁瑞,余增亮.2004.枯草芽孢杆菌JA抗菌物特性的研究及抗菌肽的分离纯化.微生物学报,44(4),511-514.
别小妹,吕凤霞,陆兆新,黄现青,沈娟.2006. Bacillus subtilis fmbJ脂肽类抗菌物质的分离和鉴定.生物工程学报,22(4),644-649.
吕应年,杨世忠,牟伯中.2005.脂肽的分离纯化与结构研究.微生物学通报,32(1),67-73.
孙力军.2006.植物内生菌Bacillus amyliliquefaciens ES-2的分离筛选及其抗菌物质的研究.南京：南京农业大学.
罗楚平,王晓宇,陈志谊,刘永锋,张杰,刘邮洲,聂亚锋,余俊杰,尹小乐.2010.枯草芽孢杆菌Bs916中脂肽抗生素Bacillomycin L的操纵子结构及生物活性.中国农业科学,43(22),4624-4634.
邓建良,刘红彦,刘玉霞,刘新涛,倪云霞,高素霞,李国庆.2010.解淀粉芽孢杆菌YN-1抑制植物病原真菌活性物质鉴定.植物病理学报(002),202-209.
韩玉竹,张淑霞,时安东,杨成旭,黄建国.2012.拉巴豆在不同土壤上的结瘤效应,饲用品质.植物营养与肥料学报,18(4),965-973.
Aranda, F.J., Teruel, J.A., Ortiz, A.2005. Further aspects on the hemolytic activity of the antibiotic lipopeptide iturin A. Biochimica et Biophysica Acta (BBA)-Biomembranes,1713(1),51-56.
Arrebola, E., Jacobs, R., Korsten, L.2010. Iturin A is the principal inhibitor in the biocontrol activity of Bacillus amyloliquefaciens PPCB004 against postharvest fungal pathogens. Journal of Applied Microbiology,108(2),386-395.
Asaka, O., Shoda, M.1996. Biocontrol of Rhizoctonia solani damping-off of tomato with Bacillus subtilis RB14. Applied and Environmental Microbiology,62(11),4081-4085.
Bais, H.P., Fall, R., Vivanco, J.M.2004. Biocontrol of Bacillus subtilis against infection of Arabidopsis roots by Pseudomonas syringae is facilitated by biofilm formation and surfactin production. Plant Physiology,134(1),307-319.
Batut, J., Howell, M., Hill, C.S.2007. Kinesin-mediated transport of Smad2 is required for signaling in response to TGF-β ligands. Developmental Cell,12(2),261-274.
Baysal, O., Caliskan, M., Yesilova, O.2008. An inhibitory effect of a new< Bacillus subtilis strain (EU07) against Fusarium oxysporum f. sp. radicis-lycopersici. Physiological and Molecular Plant Pathology,73(1),25-32.
Bedford, L., Paine, S., Sheppard, P.W., Mayer, R.J., Roelofs, J.2010. Assembly, structure, and function of the 26S proteasome. Trends in Cell Biology,20(7),391-401.
Besson, F., Peypoux, F., Michel, G., Delcambe, L.1979. Antifungal activity upon Saccharomyces cerevisiae of iturin A, mycosubtilin, bacillomycin L and of their derivatives; inhibition of this antifungal activity by lipid antagonists. The Journal of antibiotics,32(8),828.
Bie, X., Lu, Z., Lu, F.2009. Identification of fengycin homologues from Bacillus subtilis with ESI-MS/CID. Journal of Microbiological Methods,79(3),272-278.
Binder, U., Bencina, M., Eigentler, A., Meyer, V, Marx, F.2011. The Aspergillus giganteus antifungal protein AFPNN5353 activates the cell wall integrity pathway and perturbs calcium homeostasis. BMC Microbiol,11,209.
Binder, U., Chu, M., Read, N.D., Marx, F.2010. The antifungal activity of the Penicillium chrysogenum protein PAF disrupts calcium homeostasis in Neurospora crassa. Eukaryot Cell,9(9),1374-82.
Brogden, K.A.2005. Antimicrobial peptides:pore formers or metabolic inhibitors in bacteria? Nat Rev Microbiol,3(3),238-50.
Bruni, R., Gianfranceschi, G., Koch, G 2004. On peptide de novo sequencing:a new approach. Journal of Peptide Science,11(4),225-234.
Candiano, G, Bruschi, M., Musante, L., Santucci, L., Ghiggeri, G.M., Carnemolla, B., Orecchia, P., Zardi, L., Righetti, P.G 2004. Blue silver:a very sensitive colloidal Coomassie G-250 staining for proteome analysis. Electrophoresis,25(9),1327-33.
Cao, Y., Zhang, Z., Ling, N., Yuan, Y., Zheng, X., Shen, B., Shen, Q.2011. Bacillus subtilis SQR 9 can control Fusarium wilt in cucumber by colonizing plant roots. Biology and Fertility of Soils,47(5), 495-506.
Carrillo, C., Teruel, J.A., Aranda, F.J., Ortiz, A.2003. Molecular mechanism of membrane permeabilization by the peptide antibiotic surfactin. Biochimica et Biophysica Acta (BBA)-Biomcmbranes,1611(1),91-97.
Catala, R., Santos, E., Alonso, J.M., Ecker, J.R., Martinez-Zapater, J.M., Salinas, J.2003. Mutations in the Ca2+/H+ transporter CAX1 increase CBF/DREB1 expression and the cold-acclimation response in Arabidopsis. Plant Cell,15(12),2940-51.
Chen, H., Wang, L., Su, C., Gong, G., Wang, P., Yu, Z.2008. Isolation and characterization of lipopeptide antibiotics produced by Bacillus subtilis. Letters in applied microbiology,47(3),180-186.
Chen, X.H., Koumoutsi, A., Scholz, R., Eisenreich, A., Schneider, K., Heinemeyer, I., Morgenstern, B., Voss, B., Hess, W.R., Reva, O., Junge, H., Voigt, B., Jungblut, P.R., Vater, J., Sussmuth, R., Liesegang, H., Strittmatter, A., Gottschalk, G., Borriss, R.2007. Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42. Nature Biotechnology,25(9),1007-14.
Cheng, S., McCleskey, F.K., Gress, M.J., Petroziello, J.M., Liu, R., Namdari, H., Beninga, K., Salmen, A., DelVecchio, V.G.1997. A PCR assay for identification of Enterococcus faecium. Journal of clinical microbiology,35(5),1248-1250.
Chiapello, M., Daghino, S., Martino, E., Perotto, S.2010. Cellular response of Fusarium oxysporum to crocidolite asbestos as revealed by a combined proteomic approach. Journal of Proteome research, 9(8),3923-3931.
Chung, S., Kong, H., Buyer, J.S., Lakshman, D.K., Lydon, J., Kim, S.-D., Roberts, D.P.2008. Isolation and partial characterization of Bacillus subtilis ME488 for suppression of soilborne pathogens of cucumber and pepper. Applied Microbiology and Biotechnology,80(1),115-123.
Deleu, M., Paquot, M., Nylander, T.2008. Effect of Fengycin, a Lipopeptide Produced by Bacillus subtilis, on Model Biomembranes. Biophysical Journal,94(7),2667-2679.
Deleu, M., Paquot, M., Nylander, T.2005. Fengycin interaction with lipid monolayers at the air-aqueous interface-implications for the effect of fengycin on biological membranes. Journal of Colloid and Interface Science,283(2),358-365.
Even, S., Lindley, N.D., Loubiere, P., Cocaign-Bousquet, M.2002. Dynamic response of catabolic pathways to autoacidification in Lactococcus lactis:transcript profiling and stability in relation to metabolic and energetic constraints. Molecular Microbiology,45(4),1143-1152.
Fang, X., Jost, R., Finnegan, P.M., Barbetti, M.J.2013. Comparative Proteome Analysis of the Strawberry-Fusarium oxysporum f. sp. fragariae Pathosystem Reveals Early Activation of Defense Responses as a Crucial Determinant of Host Resistance. Journal of Proteome research, 12(4),1772-1788.
Fernandez-Acero, F.J., Jorge, I., Calvo, E., Vallejo, I., Carbu, M., Camafeita, E., Lopez, J.A., Cantoral, J.M., Jorrin, J.2006. Two-dimensional electrophoresis protein profile of the phytopathogenic fungus Botrytis cinerea. Proteomics,6 Suppl 1, S88-96.
Gao, Q., Liu, Y., Wang, M., Zhang, J., Gai, Y, Zhu, C., Guo, X.2009. Molecular cloning and characterization of an inducible RNA-dependent RNA polymerase gene, GhRdRP, from cotton (Gossypium hirsutum L.). Molecular Biology Reports,36(1),47-56.
Gautam, P., Shankar, J., Madan, T., Sirdeshmukh, R., Sundaram, C.S., Gade, W.N., Basir, S.F., Sarma, P.U. 2008. Proteomic and transcriptomic analysis of Aspergillus fumigatus on exposure to amphotericin B. Antimicrobial Agents and Chemotherapy,52(12),4220-7.
Gonzalez-Fernandez, R., Prats, E., Jorrin-Novo, J.V.2010. Proteomics of plant pathogenic fungi. BioMed Research International,2010.
Gonzalez-Fernandez, R., Jorrin-Novo, J.V.2011. Contribution of proteomics to the study of plant pathogenic fungi. Journal of Proteome research,11(1),3-16.
Gonzalez, F.J.2005. Role of cytochromes P450 in chemical toxicity and oxidative stress:studies with CYP2E1. Mutation Research,569(1-2),101-10.
Grau, A., Ortiz, A., de Godos, A., Gomez-Fernandez, J.C.2000. A biophysical study of the interaction of the lipopeptide antibiotic iturin A with aqueous phospholipid bilayers. Archives of Biochemistry and Biophysics,377(2),315-323.
Heerklotz, H.2008. Interactions of surfactants with lipid membranes. Q. Rev. Biophys,41(3-4),205-264.
Heerklotz, H., Seelig, J.2007. Leakage and lysis of lipid membranes induced by the lipopeptide surfactin. European Biophysics Journal,36(4-5),305-314.
Hirschi, K.D.1999. Expression of Arabidopsis CAX1 in tobacco:altered calcium homeostasis and increased stress sensitivity. The Plant Cell Online,11(11),2113-2122.
Holzapfel, W.H., Haberer, P., Geisen, R., Bjorkroth, J., Schillinger, U.2001. Taxonomy and important features of probiotic microorganisms in food and nutrition. The American journal of clinical nutrition,73(2),365s-373s.
Hubbard, P.A., Shen, A.L., Paschke, R., Kasper, C.B., Kim, J.J.2001. NADPH-cytochrome P450 oxidoreductase. Structural basis for hydride and electron transfer. Journal of Biological Chemistry, 276(31),29163-70.
Hwang, B., Cho, J., Hwang, I., Jin, H.G., Woo, E.R., Lee, D.G.2011. Antifungal activity of lariciresinol derived from Sambucus williamsii and their membrane-active mechanisms in Candida albicans. Biochemical and biophysical research communications,410(3),489-493.
Jiang, D., Zhao, L., Clapham, D.E.2009. Genome-wide RNAi screen identifies Letml as a mitochondrial Ca2+/H+ antiporter. Science,326(5949),144-7.
Jin, J., Zhang, B., Guo, H., Cui, J., Jiang, L., Song, S., Sun, M., Ren, F.2012. Mechanism analysis of acid tolerance response of bifidobacterium longum subsp. longum BBMN 68 by gene expression profile using RNA-sequencing. PLoS One,7(12), e50777.
Jorge, J.A., Polizeli, M.d.L., Thevelein, J.M., Terenzi, H.F.1997. Trehalases and trehalose hydrolysis in fungi. FEMS Microbiology Letters,154(2),165-171.
Kamiya, T., Akahori, T., Ashikari, M., Maeshima, M.2006. Expression of the vacuolar Ca2+/H+ exchanger, OsCAXla, in rice:cell and age specificity of expression, and enhancement by Ca2+. Plant and Cell Physiology,47(1),96-106.
Kim, K.J., Sung, W.S., Suh, B.K., Moon, S.K., Choi, J.S., Kim, J.G., Lee, D.G.2009a. Antifungal activity and mode of action of silver nano-particles on Candida albicans. Biometals,22(2),235-242.
Kim, K.J., Sung, W.S., Suh, B.K., Moon, S.K., Choi, J.S., Kim, J.G., Lee, D.G.2009b. Antifungal activity and mode of action of silver nano-particles on Candida albicans. BioMetals,22(2),235-42.
Kim, Y., Nandakumar, M., Marten, M.R.2007. Proteomics of filamentous fungi. Trends in Biotechnology, 25(9),395-400.
Kloepper, J., Metting Jr, F.1992. Plant growth-promoting rhizobacteria as biological control agents. Soil microbial ecology:applications in agricultural and environmental management.,255-274.
Kloepper, J., Schroth, M., Miller, T.1980. Effects of rhizosphere colonization by plant growth-promoting rhizobacteria on potato plant development and yield. Phytopathology,70(11),1078-1082.
Kusano, T., Yamaguchi, K., Berberich, T., Takahashi, Y.2007. The polyamine spermine rescues Arabidopsis from salinity and drought stresses. Plant Signal Behav,2(4),251-252.
Lakshman, D.K., Natarajan, S.S., Lakshman, S., Garrett, W.M., Dhar, A.K.2008. Optimized protein extraction methods for proteomic analysis of Rhizoctonia solani. Mycologia,100(6),867-875.
Lee, H., Churey, J., Worobo, R.2008. Purification and structural characterization of bacillomycin F produced by a bacterial honey isolate active against Byssochlamys fulva H25. Journal of Applied Microbiology,105(3),663-673.
Lee, J., Bricker, T.M., Lefevre, M., Pinson, S.R., Oard, J.H.2006. Proteomic and genetic approaches to identifying defence-related proteins in rice challenged with the fungal pathogen Rhizoctonia solani. Mol Plant Pathol,7(5),405-16.
Lee, S.C., Kim, S.H., Park, I.H., Chung, S.Y., Choi, Y. L.2007. Isolation and structural analysis of bamylocin A, novel lipopeptide from Bacillus amyloliquefaciens LP03 having antagonistic and crude oil-emulsifying activity. Archives of Microbiology,188(4),307-312.
Leiter, E., Szappanos, H., Oberparleiter, C., Kaiserer, L., Csernoch, L., Pusztahelyi, T., Emri, T., Pocsi, I., Salvenmoser, W., Marx, F.2005. Antifungal protein PAF severely affects the integrity of the plasma membrane of Aspergillus nidulans and induces an apoptosis-like phenotype. Antimicrobial agents and chemotherapy,49(6),2445-2453.
Maget-Dana, R., Peypoux, F.1994. Iturins, a special class of pore-forming lipopeptides:biological and physicochemical properties. Toxicology,87(1),151-174.
Maget-Dana, R., Thimon, L., Peypoux, F., Ptak, M.1992. Surfactin/iturin A interactions may explain the synergistic effect of surfactin on the biological properties of iturin A. Biochimie,74(12), 1047-1051.
Mahenthiralingam, E., Simpson, D.A., Speert, D.P.1997. Identification and characterization of a novel DNA marker associated with epidemic Burkholderia cepacia strains recovered from patients with cystic fibrosis. Journal of Clinical Microbiology,35(4),808-816.
Mei, H., Zhao, J., Pittman, J.K., Lachmansingh, J., Park, S., Hirschi, K.D.2007. In planta regulation of the Arabidopsis Ca(2+)/H(+) antiporter CAX1. J Exp Bot,58(12),3419-27.
Miki, H., Okada, Y, Hirokawa, N.2005. Analysis of the kinesin superfamily:insights into structure and function. Trends in Cell Biology,15(9),467-76.
Misteli, T., Caceres, J.F., Spector, D.L.1997. The dynamics of a pre-mRNA splicing factor in living cells. Nature,387(6632),523-527.
Mohammadipour, M., Mousivand, M., Salehi Jouzani, G., Abbasalizadeh, S.2009. Molecular and biochemical characterization of Iranian surfactin-producing Bacillus subtilis isolates and evaluation of their biocontrol potential against Aspergillus flavus and Colletotrichum gloeosporioides. Canadian Journal of Microbiology,55(4),395-404.
Munoz, A., Gandia, M., Harries, E., Carmona, L., Read, N.D., Marcos, J.F.2013. Understanding the mechanism of action of cell-penetrating antifungal peptides using the rationally designed hexapeptide PAF26 as a model. Fungal Biology Reviews,26(4),146-155.
Munoz, A., Marcos, J.F., Read, N.D.2012. Concentration-dependent mechanisms of cell penetration and killing by the de novo designed antifungal hexapeptide PAF26. Molecular Microbiology,85(1), 89-106.
Munoz, A., Lopez-Garcia, B., Marcos, J.F.2006. Studies on the mode of action of the antifungal hexapeptide PAF26. Antimicrobial agents and chemotherapy,50(11),3847-3855.
O'Shaughnessy, E.M., Lyman, C.A., Walsh, T.J.2009. Amphotericin B:Polyene resistance mechanisms. Antimicrobial Drug Resistance,295-305.
Ongena, M., Jacques, P.2008. Bacillus lipopeptides:versatile weapons for plant disease biocontrol. Trends in Microbiology,16(3),115-125.
Ongena, M., Jourdan, E., Adam, A., Paquot, M., Brans, A., Joris, B., Arpigny, J.L., Thonart, P.2007. Surfactin and fengycin lipopeptides of Bacillus subtilis as elicitors of induced systemic resistance in plants. Environmental Microbiology,9(4),1084-1090.
Oppenheimer, D.G., Pollock, M.A., Vacik, J., Szymanski, D.B., Ericson, B., Feldmann, K., Marks, M.D. 1997. Essential role of a kinesin-like protein in Arabidopsis trichome morphogenesis. Proceedings of the National Academy of Sciences,94(12),6261-6266.
Palma-Guerrero, J., Lopez-Jimenez, J., Perez-Berna, A., Huang, I.C., Jansson, H.B., Salinas, J., Villalain, J., Read, N., Lopez-Llorca, L.2010. Membrane fluidity determines sensitivity of filamentous fungi to chitosan. Molecular microbiology,75(4),1021-1032.
Pandey, S.P., Baldwin, I.T.2007. RNA-directed RNA polymerase 1 (RdRl) mediates the resistance of Nicotiana attenuata to herbivore attack in nature. Plant Journal,50(1),40-53.
Park, C.B., Kim, H.S., Kim, S.C.1998. Mechanism of action of the antimicrobial peptide buforin II: buforin Ⅱ kills microorganisms by penetrating the cell membrane and inhibiting cellular functions. Biochemical and biophysical research communications,244(1),253-257.
Parrou, J.L., Jules, M., Beltran, G., Francois, J.2005. Acid trehalase in yeasts and filamentous fungi: localization, regulation and physiological function. FEMS Yeast Res,5(6-7),503-11.
Parveen, M., Hasan, M.K., Takahashi, J., Murata, Y., Kitagawa, E., Kodama, O., Iwahashi, H.2004. Response of Saccharomyces cerevisiae to a monoterpene:evaluation of antifungal potential by DNA microarray analysis. Journal of Antimicrobial Chemotherapy,54(1),46-55.
Pena, L.B., Pasquini, L.A., Tomaro, M.L., Gallego, S.M.2007.20S proteasome and accumulation of oxidized and ubiquitinated proteins in maize leaves subjected to cadmium stress. Phytochemistry, 68(8),1139-46.
Qin, G., Tian, S., Chan, Z., Li, B.2007. Crucial role of antioxidant proteins and hydrolytic enzymes in pathogenicity of Penicillium expansum:analysis based on proteomics approach. Mol Cell Proteomics,6(3),425-38.
Raaijmakers, J.M., de Bruijn, I., de Kock, M.J.2006. Cyclic lipopeptide production by plant-associated Pseudomonas spp.:diversity, activity, biosynthesis, and regulation. Molecular plant-microbe interactions,19(7),699-710.
Raaijmakers, J.M., De Bruijn, I., Nybroe, O., Ongena, M.2010. Natural functions of lipopeptides from Bacillus and Pseudomonas:more than surfactants and antibiotics. FEMS Microbiology Reviews, 34(6),1037-1062.
REINHECKEL, T., SITTE, N., ULLRICH, O., KUCKELKORN, U., Davies, K., GRUNE, T.1998. Comparative resistance of the 20S and 26S proteasome to oxidative stress. Biochemical Journal, 335,637-642.
Romero, D., De Vicente, A., Olmos, J., Davila, J., Perez-Garcia, A.2007a. Effect of lipopeptides of antagonistic strains of Bucillus subtilis on the morphology and ultrastructure of the cucurbit fungal pathogen Podosphaera fusca. Journal of Applied Microbiology,103(4),969-976.
Romero, D., de Vicente, A., Rakotoaly, R.H., Dufour, S.E., Veening, J.-W., Arrebola, E., Cazorla, F.M., Kuipers, O.P., Paquot, M., Perez-Garcia, A.2007b. The iturin and fengycin families of lipopeptides are key factors in antagonism of Bacillus subtilis toward Podosphaera fusca. Molecular plant-microbe interactions,20(4),430-440.
Saharan, B., Nehra, V.2011. Plant growth promoting rhizobacteria:a critical review. Life Sci Med Res,21, 1-30.
Schmittgen, T.D., Livak, K.J.2008. Analyzing real-time PCR data by the comparative CT method. Nature protocols,3(6),1101-1108.
Schoch, C.L., Briining, A.R., Entian, K.-D., Pretorius, G.H., Prior, B.A.1997. ASaccharomyces cerevisiae mutant defective in the kinesin-like protein Kar3 is sensitive to NaCl-stress. Current Genetics, 32(5),315-322.
Shen, Q.-T., Chen, X.-L., Sun, C.-Y., Zhang, Y.-Z.2004. Dissecting and exploiting nonribosomal peptide synthetases. Acta biochimica et biophysica Sinica,36(4),243-249.
Shi, C., Zhang, Y., Bian, K., Xu, L.2010. Amount and activity changes of 20S proteasome modified by oxidation in salt-treated wheat root tips.Acta Physiologiae Plantarum,33(4),1227-1237.
Shimizu, M., Fujii, T., Masuo, S., Fujita, K., Takaya, N.2009. Proteomic analysis of Aspergillus nidulans cultured under hypoxic conditions. Proteomics,9(1),7-19.
Sobhanian, H., Razavizadeh, R., Nanjo, Y., Ehsanpour, A.A., Jazii, F.R., Motamed, N., Komatsu, S.2010. Proteome analysis of soybean leaves, hypocotyls and roots under salt stress. Proteome Sci,8,19.
Strieker, M., Tanovic, A., Marahiel, M.A.2010. Nonribosomal peptide synthetases:structures and dynamics. Current Opinion in Structural Biology,20(2),234-240.
te Welscher, Y.M., Hendrik, H., Balague, M.M., Souza, C.M., Riezman, H., De Kruijff, B., Breukink, E. 2008. Natamycin blocks fungal growth by binding specifically to ergosterol without permeabilizing the membrane. Journal of Biological Chemistry,283(10),6393-6401.
Teixeira, M.C., Santos, P.M., Fernandes, A.R., Sa-Correia, I.2005. A proteome analysis of the yeast response to the herbicide 2,4-dichlorophenoxyacetic acid. Proteomics,5(7),1889-901.
Theis, T., Marx, F., Salvenmoser, W., Stahl, U., Meyer, V.2005. New insights into the target site and mode of action of the antifungal protein of Aspergillus giganteus Research in microbiology,156(1), 47-56.
Theis, T., Wedde, M., Meyer, V., Stahl, U.2003. The antifungal protein from Aspergillus giganteus causes membrane permeabilization.Antimicrobial agents and chemotherapy,47(2),588-593.
Thevissen, K., Terras, F.R.G., Broekaert, W.F.1999. Permeabilization of fungal membranes by plant defensins inhibits fungal growth. Applied and environmental microbiology,65(12),5451-5458.
Thimon, L., Peypoux, F., Wallach, J., Michel, G.1995. Effect of the lipopeptide antibiotic, iturin A, on morphology and membrane ultrastructure of yeast cells. FEMS Microbiology Letters,128(2), 101-106.
Topanurak, S., Sinchaikul, S., Phutrakul, S., Sookkheo, B., Chen, S.T.2005. Proteomics viewed on stress response of thermophilic bacterium Bacillus stearothermophilus TLS33. Proteomics,5(14), 3722-30.
Trevors, J.2003. Fluorescent probes for bacterial cytoplasmic membrane research. Journal of biochemical and biophysical methods,57(2),87-103.
van der Weerden, N.L., Hancock, R.E.W., Anderson, M.A.2010. Permeabilization of fungal hyphae by the plant defensin NaDl occurs through a cell wall-dependent process. Journal of Biological Chemistry,285(48),37513-37520.
van der Weerden, N.L., Lay, F.T., Anderson, M.A.2008. The plant defensin, NaDl, enters the cytoplasm of Fusarium oxysporum hyphae. Journal of Biological Chemistry,283(21),14445-14452.
Vater, J., Kablitz, B., Wilde, C., Franke, P., Mehta, N., Cameotra, S.S.2002. Matrix-assisted laser desorption ionization-time of flight mass spectrometry of lipopeptide biosurfactants in whole cells and culture filtrates of Bacillus subtilis C-1 isolated from petroleum sludge. Applied and environmental microbiology,68(12),6210-6219.
Volpon, L., Besson, F., Lancelin, J.M.1999. NMR structure of active and inactive forms of the sterol-dependent antifungal antibiotic bacillomycin L. European Journal of Biochemistry,264(1), 200-210.
Waditee, R., Hossain, G.S., Tanaka, Y., Nakamura, T., Shikata, M., Takano, J., Takabe, T.2004. Isolation and functional characterization of Ca2+/H+ antiporters from cyanobacteria. Journal of Biological Chemistry,279(6),4330-8.
Yan, X., Liang, X., Jin, S., Lv, J., Yu, C., Qi, W., Li, B., Yuan, H., Qi, S., Shi, Y.2010. Primary Study on Mode of Action for Macrocyclic Fungicide Candidates (7B3, D1) against Rhizoctonia solani Kuhn(?). Journal of agricultural and food chemistry,58(5),2726-2729.
Yan, X., Qin, W., Sun, L., Qi, S., Yang, D., Qin, Z., Yuan, H.2009. Study of Inhibitory Effects and Action Mechanism of the Novel Fungicide Pyrimorph against Phytophthora capsici(?). Journal of agricultural and food chemistry,58(5),2720-2725.
Yang, H., Wang, M., Gao, Z., Zhu, C., Guo, X.2011. Isolation of a novel RNA-dependent RNA polymerase 6 from Nicotiana glutinosa, NgRDR6, and analysis of its response to biotic and abiotic stresses. Molecular Biology Reports,38(2),929-37.
Yonezawa, A., Kuwahara, J., Fujii, N., Sugiura, Y.1992. Binding of tachyplesin I to DNA revealed by footprinting analysis:significant contribution of secondary structure to DNA binding and implication for biological action. Biochemistry,31(11),2998-3004.
Yoshida, M., Muneyuki, E., Hisabori, T.2001. ATP synthase-a marvellous rotary engine of the cell. Nature Reviews Molecular Cell Biology,2(9),669-677.
Yu, G, Sinclair, J., Hartman, G., Bertagnolli, B.2002. Production of iturin A by Bacillus amyloliquefaciens suppressing Rhizoctonia solani. Soil Biology and Biochemistry,34(7),955-963.
Yuan, B., Wang, Z., Qin, S., Zhao, G.H., Feng, Y.J., Wei, L.H., Jiang, J. H.2012. Study of the anti-sapstain fungus activity of Bacillus amyloliquefaciens CGMCC 5569 associated with Ginkgo biloba and identification of its active components. Bioresource technology,114(0),536-541.
Zahler, A.M., Lane, W.S., Stolk, J.A., Roth, M.B.1992. SR proteins:a conserved family of pre-mRNA splicing factors. Genes & Development,6(5),837-847.
Zhao, Z., Wang, Q., Wang, K., Brian, K., Liu, C., Gu, Y.2010. Study of the antifungal activity of Bacillus vallismortis ZZ185 in vitro and identification of its antifungal components. Bioresource Technology,101(1),292-297.
王英国,王军华,权春善,范圣第.2007.解淀粉芽孢杆菌抗菌活性物质的分离纯化及抑菌活性研究.中国生物工程杂志,27(12),41-45.
王帅.2009.芽孢杆菌及其脂肽类化合物防治植物病害和促进植物生长的研究,南京：南京农业大学.
林东,徐庆.2001.枯草芽孢杆菌S0113分泌蛋白的抑菌作用及抗菌蛋白的分离纯化.农业生物技 术学报,9(1),77-80.
胡梁斌,周威,章挺,杨志敏,徐剑宏,石志琦.2010.抗菌肽Fengycins抑制串珠镰刀菌的初步机制.微生物学通报,37(2),251-255.
徐杨,王楠,李伟,胡江春,胡芳欣,刘丽,王书锦.2009.海洋枯草芽孢杆菌3512A抗真菌脂肽的分离纯化及结构特性鉴定.中国生物防治,25(4),328-333.
高伟,田黎,周俊英,史振平,郑立,崔志松,李元广.2009.海洋芽孢杆菌(Bacillus marinus) B-9987菌株抑制病原真菌机理.微生物学报(11),1494-1501.
刘静,王军,姚建铭,潘仁瑞,余增亮.2004.枯草芽孢杆菌JA抗菌物特性的研究及抗菌肽的分离纯化.微生物学报,44(4),511-514.
别小妹,吕凤霞,陆兆新,黄现青,沈娟.2006. Bacillus subtilis fmbJ脂肽类抗菌物质的分离和鉴定.生物工程学报,22(4),644-649.
吕应年,杨世忠,牟伯中.2005.脂肽的分离纯化与结构研究.微生物学通报,32(1),67-73.
孙力军.2006.植物内生菌Bacillus amyliliquefaciens ES-2的分离筛选及其抗菌物质的研究.南京：南京农业大学.
罗楚平,王晓宇,陈志谊,刘永锋,张杰,刘邮洲,聂亚锋,余俊杰,尹小乐.2010.枯草芽孢杆菌Bs916中脂肽抗生素Bacillomycin L的操纵子结构及生物活性.中国农业科学,43(22),4624-4634.
邓建良,刘红彦,刘玉霞,刘新涛,倪云霞,高素霞,李国庆.2010.解淀粉芽孢杆菌YN-1抑制植物病原真菌活性物质鉴定.植物病理学报(002),202-209.
韩玉竹,张淑霞,时安东,杨成旭,黄建国.2012.拉巴豆在不同土壤上的结瘤效应,饲用品质.植物营养与肥料学报,18(4),965-973.