含DCOIT以及载锌、银抗菌剂的EVA发泡材料的防霉抗菌性能研究
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摘要
以EVA为基本原料,DCOIT、载锌抗菌剂、载银磷酸锆为抗菌剂,分别制备抗菌EVA发泡材料。采用硼酸酯偶联剂分别对载锌抗菌剂和载银磷酸锆进行表面处理,分析评价了抗菌粉体的表面改性效果;进行了抗菌剂抑菌环测定,抑制孢子萌发试验,自然集菌试验与毒性试验,通过透射电镜观察抗菌剂对细菌细胞形态的影响,同时分析检测了EVA发泡材料的力学性能,防霉抗菌性能以及耐水、耐候持效防霉抗菌性能,并研究探讨了CeO_2及La_2O_3掺杂复合载银磷酸锆对EVA发泡材料抗菌性能的影响。
结果表明,DCOIT、载锌抗菌剂及其EVA发泡材料对金黄色葡萄球菌、大肠杆菌、绳状青霉、球毛壳、黑曲霉、出芽短埂霉以及自然存在的多种菌均有明显的抑制作用;载银磷酸锆及其EVA制品具有优异的抗细菌效果,但防霉能力相对较弱;在DCOIT与载银磷酸锆的分别作用下,细菌胞体形态发生了显著变化,细胞壁断裂,出现菌体空化现象;La_2O_3、CeO_2掺杂复合载银磷酸锆的加入,能够明显提高EVA发泡材料的抗菌能力,且抗菌持效性能优良;添加经硼酸酯偶联剂处理后的抗菌粉体以及含CeO_2、La_2O_3掺杂复合载银磷酸锆的EVA发泡材料,力学性能优于普通EVA发泡材料;载锌抗菌剂、载银磷酸锆对雌、雄小白鼠经口LD_(50)＞5000 mg/kg(体重),属于实际无毒类。
With EVA (ethylene-vinyl acetate copolymer) as raw materials and DCOIT (4,5-dichloro-2-n-octyl-3-isothiazolone), zinc-loaded antimicrobial and silver-loaded zirconium phosphate as antimicrobial agents, the antimicrobial EVA foamed materials were prepared. The surface modification of zinc-loaded antimicrobial and silver-loaded zirconium phosphate was conducted by using borate as coupling agent, and the treatments were evaluated. Measurement of paper bacterial(fungal) inhibition zone, spore germination test, nature mould collection and toxicity test was carried out on the antimicrobial agents and the influence of bacterium cell was observed by the transmission electron microscope (TEM). Meanwhile, the mechanical, antifungal and antibacterial properties of the antimicrobial EVA foamed materials were mensurated and the water and weather resistance of persistent antimicrobial effects were analyzed. In addition, the antimicrobial EVA foamed materials were prepared through development of CeO_2 and La_2O_3 doped silver-loaded zirconium phosphate as antimicrobial agents, and the influence on the antibacterial properties of the EVA samples produced by the mentioned antimicrobial agents was investigated.
The results indicated that DCOIT and zinc-loaded antimicrobial together with their EVA foamed materials had conspicuous inhibitory effect on Staphylococcus aureus, Escherichia coli, Penicillium funiculosum, Chaetomium globosum, Aspergillus niger, Aureobasidium pullulans and many other kinds of mould existing in nature; Silver-loaded zirconium phosphate and its EVA products were of powerful effect against bacterium, but the inhibitory effect on mould was relatively weak; DCOIT and Silver-loaded zirconium phosphate made the cell appearance of bacterium change conspicuously, the cell walls were disruptive and the cells were cavitate; The presence of La_2O_3 and CeO_2 doped silver-loaded zirconium phosphate in the EVA foamed materials resulted in the significant improvement of the antibacterial properties, and the persistent antibacterial effects of the samples were excellent; The mechanical properties of the antimicrobial EVA foamed materials which contained antimicrobial powder that was suface-modified by borate coupling agent were superior to the normal EVA foamed materials, and the addition of CeO_2、La_2O_3 doped silver-loaded zirconium phosphate showed the similar results; The oral LD50 of zinc-loaded antimicrobial and Silver-loaded zirconium phosphate in male and female mice was >5000 mg/kg, so the antimicrobials were of nontoxicity.
结果表明,DCOIT、载锌抗菌剂及其EVA发泡材料对金黄色葡萄球菌、大肠杆菌、绳状青霉、球毛壳、黑曲霉、出芽短埂霉以及自然存在的多种菌均有明显的抑制作用;载银磷酸锆及其EVA制品具有优异的抗细菌效果,但防霉能力相对较弱;在DCOIT与载银磷酸锆的分别作用下,细菌胞体形态发生了显著变化,细胞壁断裂,出现菌体空化现象;La_2O_3、CeO_2掺杂复合载银磷酸锆的加入,能够明显提高EVA发泡材料的抗菌能力,且抗菌持效性能优良;添加经硼酸酯偶联剂处理后的抗菌粉体以及含CeO_2、La_2O_3掺杂复合载银磷酸锆的EVA发泡材料,力学性能优于普通EVA发泡材料;载锌抗菌剂、载银磷酸锆对雌、雄小白鼠经口LD_(50)＞5000 mg/kg(体重),属于实际无毒类。
With EVA (ethylene-vinyl acetate copolymer) as raw materials and DCOIT (4,5-dichloro-2-n-octyl-3-isothiazolone), zinc-loaded antimicrobial and silver-loaded zirconium phosphate as antimicrobial agents, the antimicrobial EVA foamed materials were prepared. The surface modification of zinc-loaded antimicrobial and silver-loaded zirconium phosphate was conducted by using borate as coupling agent, and the treatments were evaluated. Measurement of paper bacterial(fungal) inhibition zone, spore germination test, nature mould collection and toxicity test was carried out on the antimicrobial agents and the influence of bacterium cell was observed by the transmission electron microscope (TEM). Meanwhile, the mechanical, antifungal and antibacterial properties of the antimicrobial EVA foamed materials were mensurated and the water and weather resistance of persistent antimicrobial effects were analyzed. In addition, the antimicrobial EVA foamed materials were prepared through development of CeO_2 and La_2O_3 doped silver-loaded zirconium phosphate as antimicrobial agents, and the influence on the antibacterial properties of the EVA samples produced by the mentioned antimicrobial agents was investigated.
The results indicated that DCOIT and zinc-loaded antimicrobial together with their EVA foamed materials had conspicuous inhibitory effect on Staphylococcus aureus, Escherichia coli, Penicillium funiculosum, Chaetomium globosum, Aspergillus niger, Aureobasidium pullulans and many other kinds of mould existing in nature; Silver-loaded zirconium phosphate and its EVA products were of powerful effect against bacterium, but the inhibitory effect on mould was relatively weak; DCOIT and Silver-loaded zirconium phosphate made the cell appearance of bacterium change conspicuously, the cell walls were disruptive and the cells were cavitate; The presence of La_2O_3 and CeO_2 doped silver-loaded zirconium phosphate in the EVA foamed materials resulted in the significant improvement of the antibacterial properties, and the persistent antibacterial effects of the samples were excellent; The mechanical properties of the antimicrobial EVA foamed materials which contained antimicrobial powder that was suface-modified by borate coupling agent were superior to the normal EVA foamed materials, and the addition of CeO_2、La_2O_3 doped silver-loaded zirconium phosphate showed the similar results; The oral LD50 of zinc-loaded antimicrobial and Silver-loaded zirconium phosphate in male and female mice was >5000 mg/kg, so the antimicrobials were of nontoxicity.
引文
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