多胎多浪羊及其F1高繁殖力候选基因研究
|
摘要
以374只多胎多浪羊为研究素材,选用骨形态发生蛋白受体IB基因(BMPR-IB)、骨形态发生蛋白15( BMP15()FecX~I位点)、视黄醇结合蛋4(RBP4)、制素α亚基(INHA)基因和视黄酸受体γ(RARG)作为高繁殖力候选基因,采用PCR-SSCP和PCR-RFLP技术检测这些基因在多胎多浪羊中的单核苷酸多态性;同时结合测序分析结果,研究它们对多胎多浪羊繁殖力的效应,探索这五个候选基因与多胎多浪羊高繁殖力的关系。对于检测出具有多态性的高繁殖力基因,检测其在254只多胎多浪羊F1中的多态性,同时检测杂交的其它亲本(50只塔什库尔干羊和30只萨福克羊)相应的高繁殖力基因,以便研究该高繁殖力基因在F1中的遗传和传递情况。
结果表明:BMPR-IB基因在多胎多浪羊中存在3种基因型:野生型++,突变杂合型B+和突变纯合型BB;基因型频率分别为:++基因型0.741,B+基因型0.254,BB基因型0.005;B和+等位基因频率分别为0.132和0.868。测序结果显示:BB基因型与++基因型相比在BMPR-IB基因编码区第746位碱基处发生了和Booroola绵羊相同的突变(A→G)。最小二乘法对不同基因型与产羔数的相关性分析表明:B+基因型与++基因型相比平均产羔数多0.51只(P<0.01),差异极显著。X~2适合性检验显示:BMPR-IB基因位点在多胎多浪羊中处于Hard-Weinberg平衡状态(X~2=4.22,P>0.05)。对另外四个高繁殖力基因的检测结果显示:FecX~I基因、RBP4基因、INHA基因和RARG基因的扩增片段在多胎多浪羊群体中不存在多态性。对多胎多浪羊F1 BMPR-IB基因的研究结果表明:B+基因型的理论值和实际值相差不大,基本上符合孟德尔遗传规律。
结论:BMPR-IB基因是影响多胎多浪羊多胎性的一个主效基因;并能稳定遗传给多浪羊F1代,且符合孟德尔遗传规律;FecX~I基因、RBP4基因、INHA基因和RARG基因未检测到与多浪羊多胎相关的多态性,可对这些基因的其它部位做进一步的研究。
374 prolific Duolang sheep were studied as experimental material.Single nucleotide polymorphism of bone morphogenetic protein IB receptor(BMPR-IB)gene, bone morphogenetic protein 15(BMP15) (FecX~I mutation),retinol binding protein(RBP4), inhibinα(INHA) gene and retinoic acid receptor gamma(RARG) gene were detected in prolific Duolang sheep by PCR-SSCP and PCR-RFLP technique. The detected results and the sequencing results were combined to study the effect of these genes on the prolificacy in prolific Duolang sheep and we attempted to find the link of productivity and those five candidiate genes. If one of those high prolificacy genes was detected single nucleotide polymorphism, we would detect the single nucleotide polymorphism of the high prolificacy gene in 254 prolific Duolang sheep's F1, and detect the corresponding high prolificacy gene in other cross parent(50 Tashenkuergan sheep and 30 Suffolk ), so that the high prolificacy gene's heredity and pass condition was studied in F1.
The results indicated that: The BMPR-IB gene had three genotypes in prolific Duolang sheep: Wild type ++, Heterozygous mutation B+ and Homozygous mutation BB; the genotype frequency was: 0.741, 0.254 and 0.005 respectively, and the gene frequency of B allele and + allele were: 0.132 and 0.868.The sequencing results revealed a same mutation (A→G) at 746 bp of BMPR-IB gene in prolific Duolang sheep as in Booroola sheep compared BB genotype with ++ genotype. The relationship between genotypes and litter size was analysed by means of the Least-squares method, and the result showed that prolific Duolang sheep ewes with genotype B+ had 0.51(P<0.01) lambs more than those with genotype ++ in average. The result was extremely significant difference. The chi-sequare test (X~2) showed that: for BMPR-IB gene, the popoulations of prolific Duolang sheep were in a state of Hardy-Weinberg Equilibrium (X~2= 4.22,P>0.05). The detected results of the other four high prolificacy genes showed that the products amplified of FecX~I gene, RBP4 gene, INHA gene and RARG gene had no polymorphism. The study of BMPR-IB gene in prolific Duolang sheep's F1 showed that there was little difference between B+ genotype's theoretical value and actual value, and the result basically accorded with Mendel's law of heredity.
The conclusion was that: BMPR-IB gene is a major gene which effect on the prolificacy in prolific Duolang sheep. The F1 could regularly inherit the BMPR-IB gene from prolific Duolang sheep parent and the heredity accorded with Mendel's law of heredity.The polymorphism of FecX~I gene, RBP4 gene, INHA gene and RARG gene which relation to Duolang sheep’s prolific traits were not detected.Further study on other part of the four prolificacy candidate genes will be carried on.
结果表明:BMPR-IB基因在多胎多浪羊中存在3种基因型:野生型++,突变杂合型B+和突变纯合型BB;基因型频率分别为:++基因型0.741,B+基因型0.254,BB基因型0.005;B和+等位基因频率分别为0.132和0.868。测序结果显示:BB基因型与++基因型相比在BMPR-IB基因编码区第746位碱基处发生了和Booroola绵羊相同的突变(A→G)。最小二乘法对不同基因型与产羔数的相关性分析表明:B+基因型与++基因型相比平均产羔数多0.51只(P<0.01),差异极显著。X~2适合性检验显示:BMPR-IB基因位点在多胎多浪羊中处于Hard-Weinberg平衡状态(X~2=4.22,P>0.05)。对另外四个高繁殖力基因的检测结果显示:FecX~I基因、RBP4基因、INHA基因和RARG基因的扩增片段在多胎多浪羊群体中不存在多态性。对多胎多浪羊F1 BMPR-IB基因的研究结果表明:B+基因型的理论值和实际值相差不大,基本上符合孟德尔遗传规律。
结论:BMPR-IB基因是影响多胎多浪羊多胎性的一个主效基因;并能稳定遗传给多浪羊F1代,且符合孟德尔遗传规律;FecX~I基因、RBP4基因、INHA基因和RARG基因未检测到与多浪羊多胎相关的多态性,可对这些基因的其它部位做进一步的研究。
374 prolific Duolang sheep were studied as experimental material.Single nucleotide polymorphism of bone morphogenetic protein IB receptor(BMPR-IB)gene, bone morphogenetic protein 15(BMP15) (FecX~I mutation),retinol binding protein(RBP4), inhibinα(INHA) gene and retinoic acid receptor gamma(RARG) gene were detected in prolific Duolang sheep by PCR-SSCP and PCR-RFLP technique. The detected results and the sequencing results were combined to study the effect of these genes on the prolificacy in prolific Duolang sheep and we attempted to find the link of productivity and those five candidiate genes. If one of those high prolificacy genes was detected single nucleotide polymorphism, we would detect the single nucleotide polymorphism of the high prolificacy gene in 254 prolific Duolang sheep's F1, and detect the corresponding high prolificacy gene in other cross parent(50 Tashenkuergan sheep and 30 Suffolk ), so that the high prolificacy gene's heredity and pass condition was studied in F1.
The results indicated that: The BMPR-IB gene had three genotypes in prolific Duolang sheep: Wild type ++, Heterozygous mutation B+ and Homozygous mutation BB; the genotype frequency was: 0.741, 0.254 and 0.005 respectively, and the gene frequency of B allele and + allele were: 0.132 and 0.868.The sequencing results revealed a same mutation (A→G) at 746 bp of BMPR-IB gene in prolific Duolang sheep as in Booroola sheep compared BB genotype with ++ genotype. The relationship between genotypes and litter size was analysed by means of the Least-squares method, and the result showed that prolific Duolang sheep ewes with genotype B+ had 0.51(P<0.01) lambs more than those with genotype ++ in average. The result was extremely significant difference. The chi-sequare test (X~2) showed that: for BMPR-IB gene, the popoulations of prolific Duolang sheep were in a state of Hardy-Weinberg Equilibrium (X~2= 4.22,P>0.05). The detected results of the other four high prolificacy genes showed that the products amplified of FecX~I gene, RBP4 gene, INHA gene and RARG gene had no polymorphism. The study of BMPR-IB gene in prolific Duolang sheep's F1 showed that there was little difference between B+ genotype's theoretical value and actual value, and the result basically accorded with Mendel's law of heredity.
The conclusion was that: BMPR-IB gene is a major gene which effect on the prolificacy in prolific Duolang sheep. The F1 could regularly inherit the BMPR-IB gene from prolific Duolang sheep parent and the heredity accorded with Mendel's law of heredity.The polymorphism of FecX~I gene, RBP4 gene, INHA gene and RARG gene which relation to Duolang sheep’s prolific traits were not detected.Further study on other part of the four prolificacy candidate genes will be carried on.
引文
[1]柳淑芳,姜运良,杜立新.BMPR-IB和BMP15基因作为小尾寒羊多胎性能候选基因的研究[J].遗传学报,2003,30 (8): 755-760.
[2]闫亚东,储明星,曾勇庆,等.小尾寒羊和湖羊高繁殖力候选基因BMPR-IB的研究[J].农业生物技术学报,2005,13(1):66-71.
[3]何远清,郭晓红,王金玉,等.小尾寒羊高繁殖力候选基因RBP4的研究[J].畜牧兽医学报,2006,37(7):646-649.
[4]周文然,储明星,孙少华,等.小尾寒羊高繁殖力候选基因INHA的研究[J].农业生物技术学报,2007,15(1):32-36.
[5]郭晓红,储明星,周忠孝,等.小尾寒羊高繁殖力候选基因RARG的研究[J].畜牧兽医学报,2006,37(8):756-760.
[6]柳广斌.多浪羊高繁殖力候选基因研究[D].新疆:新疆农业大学动物科学学院,2008:12-40.
[7]蒋文生.新疆多浪羊品种资源的保护与开发利用[J].中国草食动物,2006,26(2):28-30.
[8]刘志强,赵红琼,聂永梅,等.多浪羊生理生化血液流变学常值研究[J].中国血液流变学杂志[J].2001,11(1):29-31
[9]李海,饶丽娟,石长青,等.多浪羊的生理生化常值测定[J].畜禽业,2001,5:10-11
[10]陈晓军,钟发刚,罗淑萍,等.多浪羊BMPR-IB基因多态性的初步研究[J].新疆农业科学,2004,41(1):6-9.
[11]钟发刚,王新华,刘守仁,等.小尾寒羊和新疆多浪羊群体BMPR-IB基因多态性研究[J].中国草食动物,2005,25(6):15-16.
[12]史洪才,武坚,朱二勇,等.BMPR-IB基因作为新疆多浪羊多胎性能候选基因的研究[J].中国草食动物,2006,26(2):12-14.
[13]白杰,史洪才,刘明军,等.BMP15和GDF9基因作为策勒黑羊和多浪羊的多胎主效候选基因的研究[J].草食家畜(季刊),2007,2:1-4.
[14]李述刚,许宗运,侯旭杰,等.新疆多浪羊肉营养成分分析[J].肉类工业,2005,4:27-28
[15]买买提伊明?巴拉提,柳广斌,卡地尔.肉孜,等.多浪羊羊奶营养成分研究[J].中国草食动物,2009,29(1):62-63
[16]买买提明?巴拉提,米尔卡米里,早热古丽,等.麦盖提羊羔羊初生体重、体尺及其相关性的研究[J].黑龙江畜牧兽医,2001,5:9-10
[17]买买提明?巴拉提,多里坤努尔,早热古丽,等.麦盖提羊断奶体重与体尺相关性的研究[J].山西农业科学,2001,5:16-17
[18]买买提明?巴拉提,怕尔哈提,买合木提江,等.周岁麦盖提羊体重与体尺指标相关性的研究[J].四川畜牧兽医,2001,7:20-21
[19]买买提明?巴拉提,早热古丽.买买提,多里坤.努尔,等.麦盖提羊脂肪的贮积和分布特点[J].河南农业科学,2001,6:46-48
[20]买买提明?巴拉提,多里坤,早热古里,等.麦盖提羊体重与脂肪相关性的研究[J].四川畜牧兽医,2002年增刊,137(29):21-24
[21]赵红琼,刘志强,唐新燕,等.麦盖提羊皮肤结构研究初报[J].新疆农业大学学报,2001,24(3):9-12
[22]帕丽达,沙尼亚,买买提明?巴拉提.麦盖提羊羊毛含硫氨基酸的研究[J].草食家畜(季刊)2001,3:21-22
[23] PIPER L R,BINDON B.The Booroola Merino and the performance of medium nonpeppin crosses at
[1]柳淑芳,姜运良,杜立新.BMPR-IB和BMP15基因作为小尾寒羊多胎性能候选基因的研究[J].遗传学报,2003,30 (8): 755-760.
[2]闫亚东,储明星,曾勇庆,等.小尾寒羊和湖羊高繁殖力候选基因BMPR-IB的研究[J].农业生物技术学报,2005,13(1):66-71.
[3]何远清,郭晓红,王金玉,等.小尾寒羊高繁殖力候选基因RBP4的研究[J].畜牧兽医学报,2006,37(7):646-649.
[4]周文然,储明星,孙少华,等.小尾寒羊高繁殖力候选基因INHA的研究[J].农业生物技术学报,2007,15(1):32-36.
[5]郭晓红,储明星,周忠孝,等.小尾寒羊高繁殖力候选基因RARG的研究[J].畜牧兽医学报,2006,37(8):756-760.
[6]柳广斌.多浪羊高繁殖力候选基因研究[D].新疆:新疆农业大学动物科学学院,2008:12-40.
[7]蒋文生.新疆多浪羊品种资源的保护与开发利用[J].中国草食动物,2006,26(2):28-30.
[8]刘志强,赵红琼,聂永梅,等.多浪羊生理生化血液流变学常值研究[J].中国血液流变学杂志[J].2001,11(1):29-31
[9]李海,饶丽娟,石长青,等.多浪羊的生理生化常值测定[J].畜禽业,2001,5:10-11
[10]陈晓军,钟发刚,罗淑萍,等.多浪羊BMPR-IB基因多态性的初步研究[J].新疆农业科学,2004,41(1):6-9.
[11]钟发刚,王新华,刘守仁,等.小尾寒羊和新疆多浪羊群体BMPR-IB基因多态性研究[J].中国草食动物,2005,25(6):15-16.
[12]史洪才,武坚,朱二勇,等.BMPR-IB基因作为新疆多浪羊多胎性能候选基因的研究[J].中国草食动物,2006,26(2):12-14.
[13]白杰,史洪才,刘明军,等.BMP15和GDF9基因作为策勒黑羊和多浪羊的多胎主效候选基因的研究[J].草食家畜(季刊),2007,2:1-4.
[14]李述刚,许宗运,侯旭杰,等.新疆多浪羊肉营养成分分析[J].肉类工业,2005,4:27-28
[15]买买提伊明?巴拉提,柳广斌,卡地尔.肉孜,等.多浪羊羊奶营养成分研究[J].中国草食动物,2009,29(1):62-63
[16]买买提明?巴拉提,米尔卡米里,早热古丽,等.麦盖提羊羔羊初生体重、体尺及其相关性的研究[J].黑龙江畜牧兽医,2001,5:9-10
[17]买买提明?巴拉提,多里坤努尔,早热古丽,等.麦盖提羊断奶体重与体尺相关性的研究[J].山西农业科学,2001,5:16-17
[18]买买提明?巴拉提,怕尔哈提,买合木提江,等.周岁麦盖提羊体重与体尺指标相关性的研究[J].四川畜牧兽医,2001,7:20-21
[19]买买提明?巴拉提,早热古丽.买买提,多里坤.努尔,等.麦盖提羊脂肪的贮积和分布特点[J].河南农业科学,2001,6:46-48
[20]买买提明?巴拉提,多里坤,早热古里,等.麦盖提羊体重与脂肪相关性的研究[J].四川畜牧兽医,2002年增刊,137(29):21-24
[21]赵红琼,刘志强,唐新燕,等.麦盖提羊皮肤结构研究初报[J].新疆农业大学学报,2001,24(3):9-12
[22]帕丽达,沙尼亚,买买提明?巴拉提.麦盖提羊羊毛含硫氨基酸的研究[J].草食家畜(季刊)2001,3:21-22
[23] PIPER L R,BINDON B.The Booroola Merino and the performance of medium nonpeppin crosses at
[42]储明星,桑林华,王金玉,等.小尾寒羊高繁殖力候选基因BMP15和GDF9的研究[J].遗传学报,2005,32 (1):38-45.
[43] SADIGHI M,BODENSTEINER K J,BEATTIE A E, et al.Genetic mapping of ovine growth differentiation factor 9 (GDF9) to sheep chromosome 5[J].Anim Genet,2002,33 (3): 244-245.
[44]李碧侠,储明星,王金玉.绵羊GDF9基因PCR-SSCP分析[J].遗传学报,2003,30(4):307-310
[45] STEPHEN G,PHILIPPE W, VIJAYK,et al. Human estrogen receptor cDNA: sequence, expression and homology to verb-A [J]. Nature, 1986, 320 (13):134-139.
[46] ROTHSCHILD M F,LARSON R G, JACOBAON C,PEARSON P. Pvuii polymorphisms at the porcine oestrogen receptor locus(ESR) [J].Anmi Genet, 1991, 22(5):448.
[47] VAN RENS B T, VANDER LENDE T. Piglet and placental traits at term in relation to the estrogen receptor genotype in gilts[J].The riogenology,2002,57(6):1651-1667.
[48]姜运良,李宁,习欠云,等.猪雌激素受体基因(ESR)点突变的PCR-SSCP检测[J].遗传,2000,22(4):214-216
[49]陈克飞,黄路生,李宁,等.猪雌激素受体ESR基因对产仔数性状的影响[J].遗传学报,2000,27(10):853-857.
[50]朱志明,林长光,李盛霖,等.猪ESR基因位点多态性与繁殖性状相关分析[J].福建农业学报,2007,22(3):227-230.
[51]毕晓丹,储明星,金海国,等.小尾寒羊高繁殖力候选基因ESR的研究[J].遗传学报,2005,32 (10):1060-1065.
[52] MASON A J, HAYFLICK J S,LING N,et al. Complementary DNA sequences of ovarian follicular fluid inhibin show precursor structure and homology with transforming growth factor[J]. Nature,1985,318:659-663.
[53] BRUNNER R M,GOLDAMMER T,HINENDLEDER S,et al.Comparative mapping of the gene coding for inhibin-α(INHA)to chromosome 2 in sheep and cattle[J].Mammalian Genome,1995,6(4):3 09- 11.
[54] BLOMHOFF R,GREEN MH, BERG T, et al.Transport and storage of vitamin A[J]. Science, 1990,250:399-404.
[55] OLLIVIER L,MESSER LA,ROTHSCHILD MF,et al.The use of selection experiments for detecting quantitative trait loci with an application to the INRA hyperprolific pig[J]. Genet Res, 1997, 69:227 -232.
[56] ROTHSCHILD MF,MESSER L,DAY A,et al.Investigation of the retinol-binding protein4 (RBP4) gene as a candidate gene for increased litter size in pigs[J]. Mammalian Genome, 2000,11(1):75-77.
[57]杨龙,张冬杰,汪晓鸿,等.猪的RBP4基因部分序列的克隆及多态性分析[J].华北农学报,2007,22(2):11-13.
[58]张冬杰,杨龙,刘娣.野猪RBP4基因的克隆、表达及变异初步研究[J].生物学杂志,2008,25(2):40-43
[59]罗仍卓么,王立贤,孙世铎.北京黑猪RBP4基因与繁殖性状的关联分析[J].畜牧兽医学报,2008,39(5):536-539.
[60] WIRTANEN L, SEGUIN C. Cloning of cDNA sencoding retinoic acid receptors RARγ1, RARγ2, and a new splicing variant, RARγ3, from Ambystoma mexicanum and characterization of their expression during early development [J]. Biochim Bio- phys Acta, 2000, 1492:81-93.
[61] LEID M,KASTNER P, Chambon P. Multiplicity generates diversity in the retinoic acid sinalling pathways [ J]. Trends Biochem Sci,1992,17:427-433.
[62] JENKINS Z A,HENRY H M, GALLOWAY S M,et al. Comparative linkage mapping of genes on sheep chromosome 3 provides evidence of chromosomal rearrangements in the evolution of the Bovidae[J]. Cytogen Cell Genet,1997,78(3-4):272~274.
[63]储明星.绵羊高繁殖力主效基因的研究及应用[J].农业生物技术学报,2008,16(1):1-9.
[64]刘守仁,邵长发,张凤林,等.中国美利奴羊(新疆军垦型)多胎品系的选育研究[J].畜牧与兽医,1995,27(6):246-248.
[65]刘金祥,高前兆,姚树清等.多×寒×滩羊三元杂交羔羊育肥效果研究初报[J].中国草食动物,1999,1(3):17-20.
[66]王元兴,杨若飞,张有法等.肉用绵羊与湖羊杂交产羔性能的研究[J].畜牧与兽医,2003,35 (12):18-19.
[67]于佐卿,杨博辉,郭健,等.两种绵羊杂种GDF9基因和FecB基因的多态性及其与产羔数和体重关系的研究[J].中国草食动物,2008,28(2):5-8.
[68]王公金,窦德宇,花卫华,等.5个绵羊群体的BMPR-IB基因多态性分析[J].江苏农业学报,2007,23(5):447-450.
[69]白玉.DNA分子标记技术及其应用[J].安徽农业科学,2007,35(24):7422-7424.
[70]刘云芳,刻根强,王新峰.RFLP技术在动物遗传育种中的应用[J].内蒙古畜牧科学,2002,2: 17-19.
[71]雷承志.四个绵羊品种的分子遗传标记特征及其与产羔数的相关性研究[D].陕西:西北农林科技大学,2006:15-21.
[72]买买提伊明·巴拉提,柳广斌.分子标记常用技术及其在绵羊育种中的应用[J].中国畜牧兽医,2008,35(4):34-36.
[73] WILLIAMS J G,KUBELIK A R, LIVAK K J,et al. Tingey SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers [J]. Nucleic Acids Res,1990, 18(22): 6531-6535.
[74] WELSH J, MCCLELLAND M. Fingerprinting genomes using PCR with arbitrary primers [J]. Nucleic Acids Res,1990,18(24):7213-7218.
[75]曹顶国,杜立新,李淑青.4个绵羊品种随机扩增多态DNA分析[J].中国畜牧杂志,2002,38(2):12-14.
[76]代江生,刘云芳,高剑峰,等.五个绵羊品种的RAPD初步分析[J].草食家畜(季刊),1998,1:11-13.
[77]李天俊,王英超,张坤婷,等.不同品种绵羊杂交后代RAPD的分析[J].华北农学报,2007,22(1):108-111.
[78] ZIETKIEWICZ E, RAFALSKI A,LABUDA D. Genome fingerprinting by simple sequence repeat (SSR) anchored polymerase chain reaction amplification [J]. Genomics, 1994,20(2): 176-183.
[79]梁宏伟,昝林森等.微卫星标记与牛的遗传育种[J].黄牛杂志,2004,29(4):24-26.
[80]王吉振,储明星,王爱国,等.用4个微卫星标记分析7个绵羊群体之间的遗传关系[J].遗传,2004,26(5):637-643.
[81]杜立新,曹顶国.小尾寒羊微卫星与RAPD标记的研究[J].遗传学报,2003, 30 (11):1041-1044.
[82]张英杰,刘月琴,孙少华,等.利用微卫星DNA多态性预测绵羊杂种优势[J].中国草食动物,2004,2003-2004年全国养羊生产与学术研讨会议论文集:89-90.
[83]朱伟铨,王义权.AFLP分子标记技术及其在动物学研究中的应用[J].动物学杂志,2003,38 (2):101-105.
[84]贺三刚,黄锡霞,史洪才,等.超细型细毛羊基因组DNA的AFLP检测研究[J].中国畜牧兽医,2008,35(7):32-35.
[85]贺三刚,黄锡霞,田可川,等.超细型细毛羊新品系AFLP技术体系的建立[J].中国畜牧兽医,2008,35(6):40-42.
[86] ORITA M,IWAHANA H,KANAZAWA H. Detection of Po1ymorphisms of human DNA by gel electrophoresis as single-strand conformational polymorphisms[J]. Proc Natl Aead sci,USA,1989,86:2766-2770.
[87] ORITA M,SUZUKI Y,SEKIYA T,et al. A rapid and sensitive detection of point mutations and genetic polymorphism using polymerase chain reaction[J]. Genomics,1989,5:874-879.
[88]畅静桃.GDF9基因多态性及其与小尾寒羊多羔性关系的研究[D].甘肃:甘肃农业大学,2008, 15~19.
[89]杨晶,储明星,方丽,等.小尾寒羊BMP15基因多态性及其与高繁殖力关系的研究[J].农业生物技术学报,2006,14(6):870-874.
[90] J.萨姆布鲁,D.W.拉塞尔.分子克隆实验指南(第三版)[M],黄培堂,等.译.北京:科学出版社,2002:24-105;126;1564-1598.
[91]李宁.动物遗传学[M].北京:中国农业出版社,2004:139-145.
[92]桑林华.小尾寒羊高繁殖力候选基因BMPR-IA,BMP15和GDF9的研究[D].扬州大学,2005,24-25.
[93]明道绪.生物统计附试验设计[M].北京:中国农业出版社,2004:155-160.
[94]张沅,张勤.畜禽育种中的线性模型[M].北京:北京农业大学出版社,1993:52-86.
[95]李玉梅,姚纪元,吴静,等.PCR-SSCP技术的研究及应用进展[J].生物技术通报,2007,6:71-74.
[96]姜运良,李宁,赵兴波,等.影响PCR-SSCP的因素分析[J].农业生物技术学报,2008,8(3):245-247.
[97]吴泽辉.生长分化因子9基因多态性与济宁青山羊高繁殖力关系的研究[D].四川:四川农业大学硕士论文,2005:9-10.
[98]李广录,赵宗胜,薛安勇,等.湖羊繁殖性状相关候选基因的RFLP分析[J].石河子大学学报(自然科学版),2007,25(6):706-710.
[99]赵伟.RBP4,RARG,MYOG基因多态性及其与猪繁殖性能关系的研究[D].沈阳:东北农业大学,2008,8-12
[100]李娜.RBP4基因在五个猪种中的群体遗传特性分析[J].江西畜牧兽医杂志,2009,4:11-12
[101]孙延晓,曾勇庆,唐辉,等.猪PRLR和RBP4基因多态性与产仔性能的关系[J].遗传,2009,31(1):63-68
[102] JAEGER C ,HIENDLEDER S.Consmid cloning and charactization of the coding regions and regulatory elements of the ovineα-(INHA),βA-(INHBA)andβB-inhibin(INHBB)genes[J]. Animal Genetics, 1994,25(2):33.
[103] HIENDLDER S,LEWALSHI H,JAEGER C,et al.Nucleotide sequence of ovineα-inhibin(INHA) genes and evaluation of RFLP marker effects on reproductive performance [J].Animal Genetics,2002,33(3):247-248.
[104]丁朝阳,陈斌,柳小春,等.猪INHA与GnRHR基因型互作效应对产仔数的影响[J].湖南农业大学学报(自然科学版),2009,35(5):509-512.
[105]滑国华,陈世林,姚红卫,等.山羊抑制素α亚基基因HaeⅡ酶切多态性及其与产羔数性状的关联分析[J].遗传,2007,9(8):972-976.
[2]闫亚东,储明星,曾勇庆,等.小尾寒羊和湖羊高繁殖力候选基因BMPR-IB的研究[J].农业生物技术学报,2005,13(1):66-71.
[3]何远清,郭晓红,王金玉,等.小尾寒羊高繁殖力候选基因RBP4的研究[J].畜牧兽医学报,2006,37(7):646-649.
[4]周文然,储明星,孙少华,等.小尾寒羊高繁殖力候选基因INHA的研究[J].农业生物技术学报,2007,15(1):32-36.
[5]郭晓红,储明星,周忠孝,等.小尾寒羊高繁殖力候选基因RARG的研究[J].畜牧兽医学报,2006,37(8):756-760.
[6]柳广斌.多浪羊高繁殖力候选基因研究[D].新疆:新疆农业大学动物科学学院,2008:12-40.
[7]蒋文生.新疆多浪羊品种资源的保护与开发利用[J].中国草食动物,2006,26(2):28-30.
[8]刘志强,赵红琼,聂永梅,等.多浪羊生理生化血液流变学常值研究[J].中国血液流变学杂志[J].2001,11(1):29-31
[9]李海,饶丽娟,石长青,等.多浪羊的生理生化常值测定[J].畜禽业,2001,5:10-11
[10]陈晓军,钟发刚,罗淑萍,等.多浪羊BMPR-IB基因多态性的初步研究[J].新疆农业科学,2004,41(1):6-9.
[11]钟发刚,王新华,刘守仁,等.小尾寒羊和新疆多浪羊群体BMPR-IB基因多态性研究[J].中国草食动物,2005,25(6):15-16.
[12]史洪才,武坚,朱二勇,等.BMPR-IB基因作为新疆多浪羊多胎性能候选基因的研究[J].中国草食动物,2006,26(2):12-14.
[13]白杰,史洪才,刘明军,等.BMP15和GDF9基因作为策勒黑羊和多浪羊的多胎主效候选基因的研究[J].草食家畜(季刊),2007,2:1-4.
[14]李述刚,许宗运,侯旭杰,等.新疆多浪羊肉营养成分分析[J].肉类工业,2005,4:27-28
[15]买买提伊明?巴拉提,柳广斌,卡地尔.肉孜,等.多浪羊羊奶营养成分研究[J].中国草食动物,2009,29(1):62-63
[16]买买提明?巴拉提,米尔卡米里,早热古丽,等.麦盖提羊羔羊初生体重、体尺及其相关性的研究[J].黑龙江畜牧兽医,2001,5:9-10
[17]买买提明?巴拉提,多里坤努尔,早热古丽,等.麦盖提羊断奶体重与体尺相关性的研究[J].山西农业科学,2001,5:16-17
[18]买买提明?巴拉提,怕尔哈提,买合木提江,等.周岁麦盖提羊体重与体尺指标相关性的研究[J].四川畜牧兽医,2001,7:20-21
[19]买买提明?巴拉提,早热古丽.买买提,多里坤.努尔,等.麦盖提羊脂肪的贮积和分布特点[J].河南农业科学,2001,6:46-48
[20]买买提明?巴拉提,多里坤,早热古里,等.麦盖提羊体重与脂肪相关性的研究[J].四川畜牧兽医,2002年增刊,137(29):21-24
[21]赵红琼,刘志强,唐新燕,等.麦盖提羊皮肤结构研究初报[J].新疆农业大学学报,2001,24(3):9-12
[22]帕丽达,沙尼亚,买买提明?巴拉提.麦盖提羊羊毛含硫氨基酸的研究[J].草食家畜(季刊)2001,3:21-22
[23] PIPER L R,BINDON B.The Booroola Merino and the performance of medium nonpeppin crosses at
[1]柳淑芳,姜运良,杜立新.BMPR-IB和BMP15基因作为小尾寒羊多胎性能候选基因的研究[J].遗传学报,2003,30 (8): 755-760.
[2]闫亚东,储明星,曾勇庆,等.小尾寒羊和湖羊高繁殖力候选基因BMPR-IB的研究[J].农业生物技术学报,2005,13(1):66-71.
[3]何远清,郭晓红,王金玉,等.小尾寒羊高繁殖力候选基因RBP4的研究[J].畜牧兽医学报,2006,37(7):646-649.
[4]周文然,储明星,孙少华,等.小尾寒羊高繁殖力候选基因INHA的研究[J].农业生物技术学报,2007,15(1):32-36.
[5]郭晓红,储明星,周忠孝,等.小尾寒羊高繁殖力候选基因RARG的研究[J].畜牧兽医学报,2006,37(8):756-760.
[6]柳广斌.多浪羊高繁殖力候选基因研究[D].新疆:新疆农业大学动物科学学院,2008:12-40.
[7]蒋文生.新疆多浪羊品种资源的保护与开发利用[J].中国草食动物,2006,26(2):28-30.
[8]刘志强,赵红琼,聂永梅,等.多浪羊生理生化血液流变学常值研究[J].中国血液流变学杂志[J].2001,11(1):29-31
[9]李海,饶丽娟,石长青,等.多浪羊的生理生化常值测定[J].畜禽业,2001,5:10-11
[10]陈晓军,钟发刚,罗淑萍,等.多浪羊BMPR-IB基因多态性的初步研究[J].新疆农业科学,2004,41(1):6-9.
[11]钟发刚,王新华,刘守仁,等.小尾寒羊和新疆多浪羊群体BMPR-IB基因多态性研究[J].中国草食动物,2005,25(6):15-16.
[12]史洪才,武坚,朱二勇,等.BMPR-IB基因作为新疆多浪羊多胎性能候选基因的研究[J].中国草食动物,2006,26(2):12-14.
[13]白杰,史洪才,刘明军,等.BMP15和GDF9基因作为策勒黑羊和多浪羊的多胎主效候选基因的研究[J].草食家畜(季刊),2007,2:1-4.
[14]李述刚,许宗运,侯旭杰,等.新疆多浪羊肉营养成分分析[J].肉类工业,2005,4:27-28
[15]买买提伊明?巴拉提,柳广斌,卡地尔.肉孜,等.多浪羊羊奶营养成分研究[J].中国草食动物,2009,29(1):62-63
[16]买买提明?巴拉提,米尔卡米里,早热古丽,等.麦盖提羊羔羊初生体重、体尺及其相关性的研究[J].黑龙江畜牧兽医,2001,5:9-10
[17]买买提明?巴拉提,多里坤努尔,早热古丽,等.麦盖提羊断奶体重与体尺相关性的研究[J].山西农业科学,2001,5:16-17
[18]买买提明?巴拉提,怕尔哈提,买合木提江,等.周岁麦盖提羊体重与体尺指标相关性的研究[J].四川畜牧兽医,2001,7:20-21
[19]买买提明?巴拉提,早热古丽.买买提,多里坤.努尔,等.麦盖提羊脂肪的贮积和分布特点[J].河南农业科学,2001,6:46-48
[20]买买提明?巴拉提,多里坤,早热古里,等.麦盖提羊体重与脂肪相关性的研究[J].四川畜牧兽医,2002年增刊,137(29):21-24
[21]赵红琼,刘志强,唐新燕,等.麦盖提羊皮肤结构研究初报[J].新疆农业大学学报,2001,24(3):9-12
[22]帕丽达,沙尼亚,买买提明?巴拉提.麦盖提羊羊毛含硫氨基酸的研究[J].草食家畜(季刊)2001,3:21-22
[23] PIPER L R,BINDON B.The Booroola Merino and the performance of medium nonpeppin crosses at
[42]储明星,桑林华,王金玉,等.小尾寒羊高繁殖力候选基因BMP15和GDF9的研究[J].遗传学报,2005,32 (1):38-45.
[43] SADIGHI M,BODENSTEINER K J,BEATTIE A E, et al.Genetic mapping of ovine growth differentiation factor 9 (GDF9) to sheep chromosome 5[J].Anim Genet,2002,33 (3): 244-245.
[44]李碧侠,储明星,王金玉.绵羊GDF9基因PCR-SSCP分析[J].遗传学报,2003,30(4):307-310
[45] STEPHEN G,PHILIPPE W, VIJAYK,et al. Human estrogen receptor cDNA: sequence, expression and homology to verb-A [J]. Nature, 1986, 320 (13):134-139.
[46] ROTHSCHILD M F,LARSON R G, JACOBAON C,PEARSON P. Pvuii polymorphisms at the porcine oestrogen receptor locus(ESR) [J].Anmi Genet, 1991, 22(5):448.
[47] VAN RENS B T, VANDER LENDE T. Piglet and placental traits at term in relation to the estrogen receptor genotype in gilts[J].The riogenology,2002,57(6):1651-1667.
[48]姜运良,李宁,习欠云,等.猪雌激素受体基因(ESR)点突变的PCR-SSCP检测[J].遗传,2000,22(4):214-216
[49]陈克飞,黄路生,李宁,等.猪雌激素受体ESR基因对产仔数性状的影响[J].遗传学报,2000,27(10):853-857.
[50]朱志明,林长光,李盛霖,等.猪ESR基因位点多态性与繁殖性状相关分析[J].福建农业学报,2007,22(3):227-230.
[51]毕晓丹,储明星,金海国,等.小尾寒羊高繁殖力候选基因ESR的研究[J].遗传学报,2005,32 (10):1060-1065.
[52] MASON A J, HAYFLICK J S,LING N,et al. Complementary DNA sequences of ovarian follicular fluid inhibin show precursor structure and homology with transforming growth factor[J]. Nature,1985,318:659-663.
[53] BRUNNER R M,GOLDAMMER T,HINENDLEDER S,et al.Comparative mapping of the gene coding for inhibin-α(INHA)to chromosome 2 in sheep and cattle[J].Mammalian Genome,1995,6(4):3 09- 11.
[54] BLOMHOFF R,GREEN MH, BERG T, et al.Transport and storage of vitamin A[J]. Science, 1990,250:399-404.
[55] OLLIVIER L,MESSER LA,ROTHSCHILD MF,et al.The use of selection experiments for detecting quantitative trait loci with an application to the INRA hyperprolific pig[J]. Genet Res, 1997, 69:227 -232.
[56] ROTHSCHILD MF,MESSER L,DAY A,et al.Investigation of the retinol-binding protein4 (RBP4) gene as a candidate gene for increased litter size in pigs[J]. Mammalian Genome, 2000,11(1):75-77.
[57]杨龙,张冬杰,汪晓鸿,等.猪的RBP4基因部分序列的克隆及多态性分析[J].华北农学报,2007,22(2):11-13.
[58]张冬杰,杨龙,刘娣.野猪RBP4基因的克隆、表达及变异初步研究[J].生物学杂志,2008,25(2):40-43
[59]罗仍卓么,王立贤,孙世铎.北京黑猪RBP4基因与繁殖性状的关联分析[J].畜牧兽医学报,2008,39(5):536-539.
[60] WIRTANEN L, SEGUIN C. Cloning of cDNA sencoding retinoic acid receptors RARγ1, RARγ2, and a new splicing variant, RARγ3, from Ambystoma mexicanum and characterization of their expression during early development [J]. Biochim Bio- phys Acta, 2000, 1492:81-93.
[61] LEID M,KASTNER P, Chambon P. Multiplicity generates diversity in the retinoic acid sinalling pathways [ J]. Trends Biochem Sci,1992,17:427-433.
[62] JENKINS Z A,HENRY H M, GALLOWAY S M,et al. Comparative linkage mapping of genes on sheep chromosome 3 provides evidence of chromosomal rearrangements in the evolution of the Bovidae[J]. Cytogen Cell Genet,1997,78(3-4):272~274.
[63]储明星.绵羊高繁殖力主效基因的研究及应用[J].农业生物技术学报,2008,16(1):1-9.
[64]刘守仁,邵长发,张凤林,等.中国美利奴羊(新疆军垦型)多胎品系的选育研究[J].畜牧与兽医,1995,27(6):246-248.
[65]刘金祥,高前兆,姚树清等.多×寒×滩羊三元杂交羔羊育肥效果研究初报[J].中国草食动物,1999,1(3):17-20.
[66]王元兴,杨若飞,张有法等.肉用绵羊与湖羊杂交产羔性能的研究[J].畜牧与兽医,2003,35 (12):18-19.
[67]于佐卿,杨博辉,郭健,等.两种绵羊杂种GDF9基因和FecB基因的多态性及其与产羔数和体重关系的研究[J].中国草食动物,2008,28(2):5-8.
[68]王公金,窦德宇,花卫华,等.5个绵羊群体的BMPR-IB基因多态性分析[J].江苏农业学报,2007,23(5):447-450.
[69]白玉.DNA分子标记技术及其应用[J].安徽农业科学,2007,35(24):7422-7424.
[70]刘云芳,刻根强,王新峰.RFLP技术在动物遗传育种中的应用[J].内蒙古畜牧科学,2002,2: 17-19.
[71]雷承志.四个绵羊品种的分子遗传标记特征及其与产羔数的相关性研究[D].陕西:西北农林科技大学,2006:15-21.
[72]买买提伊明·巴拉提,柳广斌.分子标记常用技术及其在绵羊育种中的应用[J].中国畜牧兽医,2008,35(4):34-36.
[73] WILLIAMS J G,KUBELIK A R, LIVAK K J,et al. Tingey SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers [J]. Nucleic Acids Res,1990, 18(22): 6531-6535.
[74] WELSH J, MCCLELLAND M. Fingerprinting genomes using PCR with arbitrary primers [J]. Nucleic Acids Res,1990,18(24):7213-7218.
[75]曹顶国,杜立新,李淑青.4个绵羊品种随机扩增多态DNA分析[J].中国畜牧杂志,2002,38(2):12-14.
[76]代江生,刘云芳,高剑峰,等.五个绵羊品种的RAPD初步分析[J].草食家畜(季刊),1998,1:11-13.
[77]李天俊,王英超,张坤婷,等.不同品种绵羊杂交后代RAPD的分析[J].华北农学报,2007,22(1):108-111.
[78] ZIETKIEWICZ E, RAFALSKI A,LABUDA D. Genome fingerprinting by simple sequence repeat (SSR) anchored polymerase chain reaction amplification [J]. Genomics, 1994,20(2): 176-183.
[79]梁宏伟,昝林森等.微卫星标记与牛的遗传育种[J].黄牛杂志,2004,29(4):24-26.
[80]王吉振,储明星,王爱国,等.用4个微卫星标记分析7个绵羊群体之间的遗传关系[J].遗传,2004,26(5):637-643.
[81]杜立新,曹顶国.小尾寒羊微卫星与RAPD标记的研究[J].遗传学报,2003, 30 (11):1041-1044.
[82]张英杰,刘月琴,孙少华,等.利用微卫星DNA多态性预测绵羊杂种优势[J].中国草食动物,2004,2003-2004年全国养羊生产与学术研讨会议论文集:89-90.
[83]朱伟铨,王义权.AFLP分子标记技术及其在动物学研究中的应用[J].动物学杂志,2003,38 (2):101-105.
[84]贺三刚,黄锡霞,史洪才,等.超细型细毛羊基因组DNA的AFLP检测研究[J].中国畜牧兽医,2008,35(7):32-35.
[85]贺三刚,黄锡霞,田可川,等.超细型细毛羊新品系AFLP技术体系的建立[J].中国畜牧兽医,2008,35(6):40-42.
[86] ORITA M,IWAHANA H,KANAZAWA H. Detection of Po1ymorphisms of human DNA by gel electrophoresis as single-strand conformational polymorphisms[J]. Proc Natl Aead sci,USA,1989,86:2766-2770.
[87] ORITA M,SUZUKI Y,SEKIYA T,et al. A rapid and sensitive detection of point mutations and genetic polymorphism using polymerase chain reaction[J]. Genomics,1989,5:874-879.
[88]畅静桃.GDF9基因多态性及其与小尾寒羊多羔性关系的研究[D].甘肃:甘肃农业大学,2008, 15~19.
[89]杨晶,储明星,方丽,等.小尾寒羊BMP15基因多态性及其与高繁殖力关系的研究[J].农业生物技术学报,2006,14(6):870-874.
[90] J.萨姆布鲁,D.W.拉塞尔.分子克隆实验指南(第三版)[M],黄培堂,等.译.北京:科学出版社,2002:24-105;126;1564-1598.
[91]李宁.动物遗传学[M].北京:中国农业出版社,2004:139-145.
[92]桑林华.小尾寒羊高繁殖力候选基因BMPR-IA,BMP15和GDF9的研究[D].扬州大学,2005,24-25.
[93]明道绪.生物统计附试验设计[M].北京:中国农业出版社,2004:155-160.
[94]张沅,张勤.畜禽育种中的线性模型[M].北京:北京农业大学出版社,1993:52-86.
[95]李玉梅,姚纪元,吴静,等.PCR-SSCP技术的研究及应用进展[J].生物技术通报,2007,6:71-74.
[96]姜运良,李宁,赵兴波,等.影响PCR-SSCP的因素分析[J].农业生物技术学报,2008,8(3):245-247.
[97]吴泽辉.生长分化因子9基因多态性与济宁青山羊高繁殖力关系的研究[D].四川:四川农业大学硕士论文,2005:9-10.
[98]李广录,赵宗胜,薛安勇,等.湖羊繁殖性状相关候选基因的RFLP分析[J].石河子大学学报(自然科学版),2007,25(6):706-710.
[99]赵伟.RBP4,RARG,MYOG基因多态性及其与猪繁殖性能关系的研究[D].沈阳:东北农业大学,2008,8-12
[100]李娜.RBP4基因在五个猪种中的群体遗传特性分析[J].江西畜牧兽医杂志,2009,4:11-12
[101]孙延晓,曾勇庆,唐辉,等.猪PRLR和RBP4基因多态性与产仔性能的关系[J].遗传,2009,31(1):63-68
[102] JAEGER C ,HIENDLEDER S.Consmid cloning and charactization of the coding regions and regulatory elements of the ovineα-(INHA),βA-(INHBA)andβB-inhibin(INHBB)genes[J]. Animal Genetics, 1994,25(2):33.
[103] HIENDLDER S,LEWALSHI H,JAEGER C,et al.Nucleotide sequence of ovineα-inhibin(INHA) genes and evaluation of RFLP marker effects on reproductive performance [J].Animal Genetics,2002,33(3):247-248.
[104]丁朝阳,陈斌,柳小春,等.猪INHA与GnRHR基因型互作效应对产仔数的影响[J].湖南农业大学学报(自然科学版),2009,35(5):509-512.
[105]滑国华,陈世林,姚红卫,等.山羊抑制素α亚基基因HaeⅡ酶切多态性及其与产羔数性状的关联分析[J].遗传,2007,9(8):972-976.