HPA和NM-23在非小细胞肺癌中的表达及其相关性研究
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摘要
目的：研究乙酰肝素酶(HPA)以及转移抑制基因(NM-23)在非小细胞肺癌(NSCLC)组织中的表达,探讨它们的临床意义以及相互之间的关系。
方法：采用SYBR Green I实时荧光定量逆转录聚合酶链反应(real-time RT-PCR)方法检测HPA mRNA以及nm23-H1mRNA在48例NSCLC癌组织、10例癌旁正常肺组织以及10例肺良性疾病肺组织中的表达水平,并分析他们与临床病理参数之间的关系。
结果：在NSCLC组织、癌旁正常肺组织以及肺良性疾病肺组织均检测到HPA以及NM-23的表达。HPA mRNA在NSCLC组织平均表达水平明显高于癌旁及良性疾病肺组织(P＜0.01)；在NSCLC组织中HPA的高表达率与肿瘤病理类型(P<0.05)淋巴结转移(P<0.05)和肿瘤临床分期(P<0.01)有关,而与患者的年龄、性别、吸烟情况以及肿瘤细胞分化程度无关。nm23-H1mRNA在NSCLC组织平均表达水平低于癌旁及良性疾病肺组织(P<0.01)；在NSCLC组织中：nm23-H1mRNA的低表达率与肿瘤病理类型(P<0.05)淋巴结转移(P<0.05)、肿瘤细胞分化程度(P<0.05)以及肿瘤临床分期(P＜0.01)有关,而与患者的年龄、性别、吸烟情况以及无关。相关性分析结果提示：HPA mRNA的高表达与nm23-H1mRNA的低表达呈正相关。
结论：HPA与nm23-H1在NSCLC的发生以及侵袭、转移过程中发挥了重要作用,HPA和nm23-H1mRNA可能是反映NSCLC发生、发展、转移发生及预后的生物学标记物,有助于肺癌的疗效预测及判断预后并为肺癌基因靶向治疗提供了理论基础.
Objective To investigate the expressions of human heparanase (HPA) and non-metastasis gene (nm-23) in Non-small cell lung cancer, and explore the relationship between their expressions and tumor's clinicopathological characteristics. Methods The expression of HPA mRNA and nm23-H1mRNA in NSCLC cancer tissues(n=48), tumor adjacent tissues(n=10) and normal lung tissues(n=10) were detected by realtime reverse transcription polymerase chain reaction(realtime RT—PCR). Results The expression level of HPA mRNA(8.244±5.314) was markedly higher than that in tumor adjacent tissues(1.043±0.478) and normal lung tissues(1.025±0.465)(P＜0.01). The high expression level of HPA mRNA in the NSCLC tissues had significantly correlated with the pathology types (P＜0.05), lymphatic metastasis (P＜0.05) and TNM stage (P＜0.01). No significant differences in high HPA mRNA expression were observed with smoking, age, gender and degree of differentiation among the NSCLC patients (P＞0.05). The mean expression level of nm23-H1mRNA(0.599±0.566) was markedly lower than that in tumor adjacent tissues (1.232±0.328) and normal lung tissues(1.443±0.427)(P＜0.01).The low expression level of nm23-Hl mRNA in the NSCLC tissues correlated significantly with the pathology types (P＜0.05) lymphatic metastasis (P＜0.05),TNM stage (P＜0.01) and degree of differentiation. No significant differences in low nm23-Hl mRNA expression were observed with age, smoking, gender among the NSCLC patients (P＞0.05). The high expression of HPA mRNA had positive correlation with the low expression of nm23-Hl mRNA; Conclusion HPA and nm23-Hl gene are closely related to oncogenesis, development, and metastasis of human NSCLC, the two gene may be two important biological markers in reflecting the progression, infiltration, metastatic potential,clinical prediction and prognosis of NSCLC and provide theoretical basis of gene targeted therapy.
方法：采用SYBR Green I实时荧光定量逆转录聚合酶链反应(real-time RT-PCR)方法检测HPA mRNA以及nm23-H1mRNA在48例NSCLC癌组织、10例癌旁正常肺组织以及10例肺良性疾病肺组织中的表达水平,并分析他们与临床病理参数之间的关系。
结果：在NSCLC组织、癌旁正常肺组织以及肺良性疾病肺组织均检测到HPA以及NM-23的表达。HPA mRNA在NSCLC组织平均表达水平明显高于癌旁及良性疾病肺组织(P＜0.01)；在NSCLC组织中HPA的高表达率与肿瘤病理类型(P<0.05)淋巴结转移(P<0.05)和肿瘤临床分期(P<0.01)有关,而与患者的年龄、性别、吸烟情况以及肿瘤细胞分化程度无关。nm23-H1mRNA在NSCLC组织平均表达水平低于癌旁及良性疾病肺组织(P<0.01)；在NSCLC组织中：nm23-H1mRNA的低表达率与肿瘤病理类型(P<0.05)淋巴结转移(P<0.05)、肿瘤细胞分化程度(P<0.05)以及肿瘤临床分期(P＜0.01)有关,而与患者的年龄、性别、吸烟情况以及无关。相关性分析结果提示：HPA mRNA的高表达与nm23-H1mRNA的低表达呈正相关。
结论：HPA与nm23-H1在NSCLC的发生以及侵袭、转移过程中发挥了重要作用,HPA和nm23-H1mRNA可能是反映NSCLC发生、发展、转移发生及预后的生物学标记物,有助于肺癌的疗效预测及判断预后并为肺癌基因靶向治疗提供了理论基础.
Objective To investigate the expressions of human heparanase (HPA) and non-metastasis gene (nm-23) in Non-small cell lung cancer, and explore the relationship between their expressions and tumor's clinicopathological characteristics. Methods The expression of HPA mRNA and nm23-H1mRNA in NSCLC cancer tissues(n=48), tumor adjacent tissues(n=10) and normal lung tissues(n=10) were detected by realtime reverse transcription polymerase chain reaction(realtime RT—PCR). Results The expression level of HPA mRNA(8.244±5.314) was markedly higher than that in tumor adjacent tissues(1.043±0.478) and normal lung tissues(1.025±0.465)(P＜0.01). The high expression level of HPA mRNA in the NSCLC tissues had significantly correlated with the pathology types (P＜0.05), lymphatic metastasis (P＜0.05) and TNM stage (P＜0.01). No significant differences in high HPA mRNA expression were observed with smoking, age, gender and degree of differentiation among the NSCLC patients (P＞0.05). The mean expression level of nm23-H1mRNA(0.599±0.566) was markedly lower than that in tumor adjacent tissues (1.232±0.328) and normal lung tissues(1.443±0.427)(P＜0.01).The low expression level of nm23-Hl mRNA in the NSCLC tissues correlated significantly with the pathology types (P＜0.05) lymphatic metastasis (P＜0.05),TNM stage (P＜0.01) and degree of differentiation. No significant differences in low nm23-Hl mRNA expression were observed with age, smoking, gender among the NSCLC patients (P＞0.05). The high expression of HPA mRNA had positive correlation with the low expression of nm23-Hl mRNA; Conclusion HPA and nm23-Hl gene are closely related to oncogenesis, development, and metastasis of human NSCLC, the two gene may be two important biological markers in reflecting the progression, infiltration, metastatic potential,clinical prediction and prognosis of NSCLC and provide theoretical basis of gene targeted therapy.
引文
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