摘要
光散射技术自建立以来,已经得到了广泛的应用和发展。本文以共振光散射分析技术为主要研究手段,利用金属纳米粒子的等离子共振光散射性质建立了灵敏测定药物、生物大分子的方法,期望为药物、生物大分子的分析提供一种灵敏的测定方法,为医药、生化领域的研究提供一定的帮助;研究了荧光分析法中的两种干扰因素:拉曼散射和内滤效应,通过荧光光度计将二者结合起来建立了一种新的分析方法,并将其用于金属钴离子的测定,期望进一步开发荧光光度计的功能;共振光散射技术的选择性较差一直是阻碍其发展的不利因素,为了解决这一问题,本文初步建立了高效液相色谱和共振光散射的联用技术,期望通过二者的联用,解决共振光散射技术选择性和重现性差的问题,同时发展一种高选择性高灵敏度的分离分析技术。本文主要研究内容如下:
一.金属纳米材料由于其特殊的物理化学性质已经被广泛的应用于生化药物的分析领域,尤其是金、银纳米粒子的等离子吸收性质,已被用于DNA杂交、免疫分析等,但是它们强的光散射能力的开发却只有少量报道。本文主要将金和银纳米粒子的等离子共振光散射性质应用于药物、生物大分子分析,建立了灵敏的测定药物与生物大分子的方法。
1).在NaOH和NH_3·H_2O共存的碱性条件下,硝酸银与阿魏酸发生氧化还原反应生成银纳米粒子,产生增强的等离子共振光散射信号,根据产生的共振光散射信号和阿魏酸浓度的关系,建立了等离子共振光散射测定阿魏酸的分析方法。在最佳实验条件下,可检测阿魏酸浓度范围为0.2~2.0μmol L~(-1),检出限为15.2nmol L~(-1)。研究结果表明,该方法简单、快速、灵敏,本文为其它类似的酚酸类物质的检测提供了一种可靠的方法。此外,本文还提供了一种环境友好的合成银纳米粒子的方法。
2).在NaOH和NH_3·H_2O共同存在的碱性介质中,硝酸银可以与儿茶酚胺类药物左旋多巴发生氧化还原反应,产生银纳米粒子,进而产生增强的等离子共振光散射信号,根据信号强度和左旋多巴浓度之间的关系,我们建立了等离子共振光散射技术测定左旋多巴的方法,在最佳实验条件下,可检测左旋多巴浓度为0.2~2.0μmolL~(-1),检出限为20.1nmolL~(-1)。通过理论探讨,我们认为左旋多巴参与反应的活性基团主要是邻二酚,而儿茶酚胺类药物是以具有邻二酚的儿茶酚为母核的一类化合物,因此我们推测其它儿茶酚胺类药物,比如多巴胺、肾上腺素、去甲肾上腺素等,在一定的实验条件下都可以和硝酸银反应生成银纳米粒子而产生增强的等离子共振光散射信号,据此可以建立儿茶酚胺类药物的分析方法。通过以左旋多巴为代表,证明该分析方法简单、快速、灵敏、有效。
3).凝血酶在血栓形成过程中起重要作用,因此血浆凝血酶浓度的检测对临床疾病的诊断、病程发展、愈后以及疗效的监测和评估等都具有重要的意义。本文以金纳米粒子为探针,将巯基修饰的凝血酶适配子DNA连接于金纳米粒子表面,向该溶液中加入凝血酶后,由于凝血酶和其适配子DNA的反应,通过吸收光谱和透射电子显微镜表征,发现金纳米粒子发生了聚集,从而引起金纳米粒子的等离子共振光散射增强,基于此建立了利用等离子共振光散射技术测定凝血酶的分析方法。实验发现,金纳米粒子的等离子共振光散射信号强度与加入的凝血酶浓度在1.8~9.0nmolL~(-1)范围内呈良好的线性关系,检出限浓度为0.2nmol L~(-1),可见该方法可以灵敏的检测凝血酶。由于凝血初期产生的凝血酶浓度在纳摩尔水平,因此该方法可用于临床上凝血酶的测定。
二.在荧光分析法中,拉曼散射和内滤效应常常作为干扰因素而存在,通常需要避免或校正。本着“分析科学在于合理的利用信号”的原则,本文通过普通的荧光分光光度计将这两种干扰因素结合起来,以钴(Ⅱ)和钴试剂为研究对象,利用水的拉曼散射信号和钴试剂的内滤效应,通过钴(Ⅱ)加入前后,钴试剂对水的拉曼散射光吸收不同,即发生内滤效应的程度不同,从而引起拉曼散射信号随着钴(Ⅱ)浓度的变化而变化,通过理论推导建立了拉曼散射信号强度与钴(Ⅱ)浓度之间的线性关系,建立了基于水的拉曼散射信号和试剂的内滤效应测定钴(Ⅱ)的分析方法,该方法的检出限为1.2×10~(-7)molL~(-1),灵敏度与吸收光度法相当。该方法以最常用的溶剂水为探针,利用水的拉曼散射信号,不需要固定的激发波长,可以根据待分析样品选择合适的激发和发射波长,非常灵活、简单,并且提出了一种在荧光光度计上测定非荧光物质的新方法,进一步开发了荧光分光光度计的功能。
三.共振光散射分析技术虽然灵敏度很高,但是其选择性和重现性较差一直是使该技术不尽完美之处;而色谱分离技术的最大优势就在于它们的高分离效率和高选择性,但分析检测的灵敏度不高。本文以高效液相色谱技术为例,以胰岛素为研究对象,初步建立了高效液相色谱和共振光散射的联用技术,该联用技术集两者优势于一体,既提高了共振光散射分析技术的选择性和重现性,同时又提高了高效液相色谱的检测灵敏度,丰富了检测信号的类型。随着研究的深入和联用技术的成熟,共振光散射检测器有望发展成为高效液相色谱的通用检测器。
Since the establishment of the light scattering technique, it has applied widely and developed quickly. In this contribution, based on the resonance light scattering (RLS) technique, we have employed the plasmon resonance light scattering (PRLS) properties of metal nanoparticles and constructed methods for the determination of the Pharmaceuticals and biomacromolecules, which would provide sensitive detection methods for pharmaceuticals and biomacromolecules and may be helpful in the fields of medicine and biochemistry; Raman scattering and Inner Filter Effect (IFE) are the interference sources in spectrofluorometry, we have coupled them together reasonably and proposed a novel approach for the determination of Co(II) by spectrofluorometer, the function of spectrofluorometer can be further exploited; Poor selectivity is always the factor that limits the development of RLS technique, to solve the problem, we have also incorporated the high performance liquid chromatography (HPLC) technique and the RLS technique by employing the insulin as a model system, We hope that the combination of HPLC-RLS technique could improve the selectivity and stability of the RLS technique and then develop a new technique with high slectivity and high sensitivity. The main contents of investigation in the contribution are as follows:
Metal nanoparticles have been found the applications in the fields of pharmaceuticals and biomacromolecules analysis for their unusual physicochemical properties. Of the nanoparticles, especially gold and silver nanoparticles, their plasmon absorption properties have been widely used in the fields, such as DNA hybridization, immunoassay and so on, but the research on their PRLS properties has been little reported. In this contribution, we have focused on the PRLS properties of the nanoparticles and applied them to the pharmaceutical and biomacromolecules analysis.
1). In the coexistence of NaOH and NH_3-H_2O medium, it was found that, Ferulic Acid (FA) acts as a reducing agent and can be oxidized by AgNO_3, resulting in the formation of silver nanoparticles (NPs) which give the enhanced PRLS signals, the detection method of FA was constructed according to the linear relationship between the PRLS intensities and the concentrations of FA. Under the optimal conditions, the FA concentrations of 0.2~2.0umol L~(-1) could be detected and the detection limit was 15.2nmol L~(-1). The results have shown that the method is simple, rapid and sensitive, it also provides a reliable detection method for other phenolic compounds. What's more, the present reaction maybe provides the basis of an environmentally friendly approach for the synthesization of silver nanoparticles.
2). In the alkaline medium with the coexistence of NaOH and NH_3H_2O, one of the Catecholamines, L-DOPA can be oxidized by AgNO_3, resulting in the formation of silver NPs and the enhanced PRLS signals, the method for L-DOPA detection was constructed according to the linear relationship between the PRLS intensities and the concentrations of L-DOPA. Under the optimal conditions, the L-DOPA concentrations of 0.2~2.0μmolL~(-1) could be detected and the detection limit was 20.1nmol L~(-1). By the mechanism investigations, we conclude that the groups oxidized in L-DOPA are the two adjacent phenol groups, while all the catecholamine compounds possess the two adjacent phenol groups, so all of other catecholamine compounds, such as dopamine, adrenaline, noradrenaline, could be anticipated to be oxidized by AgNO_3 under the corresponding conditions, resulting in the formation of silver NPs and the enhanced PRLS signal, so the detection methods for the catecholamine compounds could be constructed as L-DOPA. The results of detection L-DOPA have shown that the method is simple, rapid, sensitive and reliable.
3).Thrombin plays an important role in the course of thrombus formation, so the detection of thrombin has important significances for the diseases diagnosis, evolution, evaluation of curative effect. In this contribution, gold nanoparticles (NPs) stabilized with citrate were functionalized by the thiolated thrombin binding aptamer (TBA), the gold NPs are served as a probe, when thrombin was added into the solution, the gold NPs would be aggregated for thrombin reacted with TBA, which was testified by the absorption spectra and the TEM images of gold NPs, and the then the PRLS signals were enhanced, so we set up a method for the detection of thrombin based on the PRLS signals of gold NPs. The results showed that the PRLS intensities were in proportion to the thrombin concentrations over the range of 1.8~9.0nmol L~(-1), the detection limit of thrombin was 0.2 nmol L~(-1), so it is a sensitive method for thrombin detection. During the initial phase of coagulation thrombin is produced in nanomolar concentrations, so the present method can be used for the clinical detection of thrombin.
Both Raman scattering and IFE are the interference sources and usually should be avoided or corrected in spectrofluorometry. We suppose analytical sciences lie on the reasonable application of signals only if the intensity of the signals has simple functional relationship with the content of analytes. In this contribution, we propose a quantitative method based on the IFE of reagents on the Raman scattering signals of solvent by taking the complexation of Co(II) with 4-[(5-chloro-2-pyridyl)azo]-l,3-diaminobenzene (5-C1-PADAB) as a model system on a common spectrofluorometer. In a solution of 5-C1-PADAB, the Raman scattering signals of water are decreased owing to the IFE of 5-C1-PADAB, after the addition of Co(II) which can form the complex with 5-C1-PADAB and consumes the reagent, then the Raman scattering signals were found recovered, based on the linear relationship between the enhanced Raman scattering intensities and the concentrations of Co(II), Co(II) was detected, the detection limit is 1.2×10~(-7) mol L~(-1) which is comparable to the generally used UV-Vis spectrophotometry. In the present method, the commonly used solvent, water, serves as a probe, and the Raman scattering signals of water are used, the obtain of Raman scattering does not require flexed excitaion wavelength, so we can freely select the excitation wavelength according to the analyte, which is very flexible and simple. What's more, a novel method for detection of some non-fluorescent compounds was proposed in a common spectrofluorometer, the function of spectrofluorometer was further exploited.
As we know, the RLS technique is very sensitive for the detection, but its poor selectivity and stability are the limitations for being as a perfect technique. While high separation resolution and selectivity are the advantages of chromatography all the time, the disadvantage lies in the poor detection sensitivity. Here HPLC is chosen as a model, combining the advantages of the both techniques, the incorporation of HPLC-RLS was carried out in the contribution, the incorporated technique not only improves the selectivity and stability of the RLS technique, but also enhances the detection sensitivity of HPLC and enriches the detection types of HPLC. With the deep research and developed incorporated technique, the RLS detector is hopeful for HPLC.
引文
[1] Debye, P. J. Appl. Phys. 1944, 15,388.
[2] 钱人元,施良和,史观一.化学学报,1951,23,215.
[3] Miller, G. A. J. Phys. Chem.1978, 82,616.
[4] 应琦宗,陈国庆,黄丽荣.高分子通讯,1981,1,24.
[5] 郎庆勇,李连仲.岩矿测试,1987,6,257.
[6] Tanford, c. Physical Chemistry of Macromolecules, John Wiley and Sons, Inc., New York and London, 1961, 275.
[7] Huglin, M. B. Pure and Appl. Chem. 1977, 49, 929.
[8] Tupitsyn, V. P., Khim. Tekhnol. 1988, 31, 50.
[9] Blaszczak, Z., Acta. Phys. Pol. A.1985, 68,629.
[10] Moumnt, J. R.; Bigio, I. J.; Boyer, J. et al. Laser Surg. Med. 1995, 17, 350.
[11] Perelman, L. T.; Backman, V.; Wallace, M. et al. Phys. Rev. Lett. 1998, 80, 627.
[12] Lauger, J.; Weigel, R.; Berger, K. et al. Colloid Inerface Sci. 1996, 181, 521.
[13] Mccally, R. L.; Farrell, R. A. J. Refract. Surg. 1999, 15,706.
[14] Lee, H.; Williams, S.K.R.; Allison, S. D. et al. Anal. Chem. 2001, 73, 837.
[15] Schure, M. R.; Pallkar, S. A. Anal. Chem. 2002, 74, 684.
[16] Andersson, M.; Wittgren, B.; Wahlund, K. G. Anal. Chem. 2001, 73, 4852.
[17] Yethiraj, A.; Blaaderen, A. V. Nature 2003, 421, 513.
[18] Magnuson, M. L.; Lytle, D. A.; Frietch, C. M.; et al. Anal. Chem. 2001, 73, 4815.
[19] Anglister, J.; Steinberg, I. Z. Chem. Phys. Lett. 1979, 65, 50.
[20] Knoll, D. A.; Bloomfield, V. Biochem. 1984, 23, 3358.
[21] Pasternack, R. F.; Bustamante, C.; Collings, P. J. J. Am. Chem. Soc. 1993, 115, 5393.
[22] Arena, G.; Scolaro, L. M.; Pasternack, R. F. Inorg. Chem. 1995, 34, 2994.
[23] Pasternack, R. F.; Collings, P. J. Science 1995, 269, 935.
[24] Rubires, R.; Crusats, J.; E12Hachemi, Z. New J. Chem. 1999, 23,189.
[25] Pasternack, R. E; Schaefer, K. E; Hambright, R Inorg. Chem. 1994, 33, 2062.
[26] Parkash, J.; Robblee, J. H.; Agnew, J. Biophys. J. 1998, 74, 2089.
[27] Purrello, R.; Bellacchio, E.; Gurrieri, S. J. Phys. Chem. B 1998, 102, 8852.
[28] Pasternack, R. E; Goldsmith J. I.; Szep S. Biophys. J. 1998, 5, 1024.
[29] 魏永巨,北京大学博士学位论文,1997.
[30] 李原芳,黄承志,胡小莉.分析化学,1998,26,1508.
[31] Huang, C. Z.; Li, K. A.; Tong, S. Y. Anal. Chem. 1996, 68, 2259.
[32] Huang, C. Z.; Li, K. A.; Tong, S. Y. Anal. Chem. 1997, 69, 514.
[33] Yang, J. H.; Tong, C. L.; Jie, N. Q. et al. J Pharm. Biomed. Anal. 1997, 15, 1833
[34] 刘绍璞,刘忠芳,李明.化学学报,1995,53,1178.
[35] 刘绍璞,刘忠芳,李明.化学学报,1995,53,1185.
[36] 刘绍璞,刘忠芳,李明.分析化学,1996,28,501.
[37] 刘绍璞,刘忠芳,蒋治良等.化学学报,2001,11,1864.
[38] 高建华,林鹏,陈彬等.冶金分析,2000,20,1.
[39] 刘绍璞,刘忠芳.西南师范大学学报(自然科学版),1998,24,412.
[40] 刘绍璞,杨睿,刘忠芳.分析化学,1998,26,1432.
[41] 谢济运,蒋治良.物理化学学报,2001,17,406.
[42] Huang, C. Z.; Li, Y. F.; Hu, X. L. etal. Anal. Chim. Acta 1999, 395, 187.
[43] Yao, G.; Li, K. A.; Tong, S. Y. Anal. Chim. Acta 1999, 398: 319.
[44] 姚刚,北京大学博士论文,2000,北京.
[45] 黄承志,李原芳,奉萍.分析化学,2001,29,832.
[46] Borissevitch, I. E.; Tominaga, T. T.; Imasato, H. et al. Anal. Chim. Acta 1997, 343, 281.
[47] Collings, P. J.; Gibbs, E. J.; Starr, T. E. et al. J. Phys. Chem. B 1999, 103, 8474.
[48] Micali, N.; Mallamace, F.; Castriciano, M. et al. Anal. Chem. 2001, 73, 4958.
[49] 杨传孝,西南师范大学硕士论文,2003
[50] Yang, C. X.; Huang, C. Z.; Li, Y. F. Anal. Sci., 2003, 19, 211.
[51] Yang, C. X.; Huang, C. Z.; Li, Y. F. Anal. Lett., 2002, 35, 1945.
[52] Feng, P.; Shu, W. Q.; Huang, C. Z. et al. Anal. Chem. 2001, 73, 4307.
[53] Lu, W.; Huang, C. Z.; Li, Y. F. Analyst 2002, 127, 1392.
[54] Feng, P.; Huang, C. Z.; Li, Y. F. Anal. Biochem. 2002, 308, 83.
[55] Lu, W.; Huang, C. Z.; Li, Y. F. Anal. Chim. Acta 2003, 475, 151.
[56] Pang, X. B.; Huang, C. Z.; Li, Y. F. et al. Bull. Chem. Soc. Jpn. 2003, 76, 1941.
[57] Huang, C. Z.; Lu, W.; Li, Y, F. Anal. Chim. Acta. 2003, 494, 11.
[58] Pang, X. B.; Huang, C. Z. J. Pharm. Biomedi. Anal. 2004, 35, 185.
[59] Huang, C. Z.; Feng, P.; Li, Y. F. et al. Anal. Chim. Acta, 2005, 538, 337.
[60] Huang, C. Z.; Feng, P.; Li, Y. F. Anal. Bioanal. Chem. 2005, 382, 85.
[61] Dong, L. j.; Chen, X. g.; Hu, Z. d. Talanta 2007, 71, 555.
[62] 郭宏平,西南师范大学硕士论文,2005.
[63] Huang, C. Z.; Liu, Y.; Wang, Y. H. et al. Anal. Biochem. 2002, 321, 236.
[64] Liu, X. D.; Huang, C. Z.; Guo, H. P. et al. Chin. J. Chem. 2006, 24, 89.
[65] Guo, H. P.; Huang, C. Z.; Ling, J. Chin. Chem. Lett. 2006, 17, 53.
[66] Ling, J.; Huang, C. Z.; Li, Y. F. Anal. Chim. Acta 2006, 567, 143.
[67] 庞小兵,西南师范大学硕士论文,2004.
[68] Tan, K. J.; Li, Y. F.; Huang, C. Z. Luminescence 2005, 20, 176.
[69] 代小霞,李原芳,黄承志.分析化学 2005,33,1535.
[70] Huang, C. Z.; Pang, X. B.; Li, Y. F., Anal. Lett. 2005, 38, 349-362
[71] Qi, L.; Han, Z. Q.; Chen, Y. J. Chromatogr. A 2006, 1110, 235.
[72] Long, Y. J.; Li, Y. F.; Huang, C. Z. Anal. Chim. Acta. 2005, 552, 175.
[73] Huang, C. Z.; Pang, X. B.; Li, Y. F. etal. Talanta, 2006, 69, 180.
[74] Dai, X. X.; Li, Y. F.; He, W. et al. Talanta, 2006, 70, 578.
[75] Roll, D.; Malicka, J.; Gryczynski, I. et al. Anal. Chem. 2003, 75, 3440.
[76] Aslan, K.; Lakowicz, J. R.; Geddes, C. D. Anal. Chem. 2005, 77, 2007.
[77] Liao, Q. G.; Li, Y. F.; Huang, C. Z. Talanta 2007, 71, 567.
[78] 王永红,西南大学硕士论文,2004.
[79] Wang, Y. H.; Guo, H. P.; Tan, K. J. etal. Anal. Chim. Acta 2004, 521, 109.
[80] Huang, C. Z.; Wang, Y. H.; Guo, H. P. et al. Analyst 2005, 130, 200.
[81] 谭克俊,西南大学博士论文,2006.
[82] Tan, K. J.; Li, Y. F.; Huang, C. Z. et al. Chin. Chem. Lett. 2006, 17, 679.
[83] Tan, K. J.; Huang, C. Z.; Huang, Y. M. Talanta 2006, 70, 116.
[84] 赵华文,西南大学博士论文,2006.
[85] Zhao, H. W.; Huang, C. Z.; Li, Y. F. Anal. Chim. Acta 2006, 564, 166.
[86] 陈义,齐莉,韩志强,余晓.广西师范大学学报(自然科学版),2003,21,10.
[87] Haes, A. J.; Giordano, B. C.; Collins, G. E. Anal. Chem. 2006, 78, 3758.
[88] Sanz-Nebot, V.; Benavente, F.; Barbosa, J. J. Chromatogr. A 2002, 950, 99.
[89] Suhara, Y.; Kamao, M.; Tsugawa, N. et al. Anal. Chem. 2005, 77, 757.
[90] Kuberan, B.; Lech, M.; Zhang, L. j. et al. J. Am. Chem. Soc. 2002, 124, 8707.
[91] 刘绍璞,刘忠芳.高等学校化学学报,1996,17,887.
[92] Liu, S. P.; Liu, Z. F.; Zhou, G. M. Anal. Lett. 1998, 37, 1247.
[93] 刘绍璞,刘忠芳.高等学校化学学报,1996,17,1213.
[94] Liu, S. P.; Liu, Z. F.; Huang, C. Z. Anal. Sci. 1998, 14, 799.
[95] Jiang, Z. L.; Liu, Q. Y.; Liu, S. P. Talanta 2002, 58, 635.
[96] 刘绍璞,刘忠芳,罗红群.西南师范大学学报(自然科学版),2000,25,408.
[97] 刘绍璞,周光明,刘忠芳等.高等学校化学学报,1998,19,1040.
[98] Liu, S. P.; Liu, Q.; Liu, Z. F. et al. Anal. Chim. Acta 1999, 379, 53.
[99] Liu, S. E; Liu, Z. F.; Luo, H. Q. Anal. Chim. Acta 2000, 407, 255.
[100] 邓晔,曾建强,李芳等.分析测试与仪器,2000,6,86.
[101] Zhao, Y. K.; Cao, Q. E.; Hu, Z. D. et al. Anal. Chim. Acta. 1999, 388, 45.
[102] Cao, Q. E.; Zhao, Y. K.; Yao, X. J. et al. Spectrochim. Acta. Part A. 2000, 56, 1319.
[103] Cao, Q. E.; Zhao, Y. K.; Yao, X. J. et al. Anal. Lett. 2001, 34, 773.
[104] 蒋治良,李芳,梁宏,化学学报,2000,58,1059.
[105] Jiang, Z. L.; Liu, Q. Y.; Liu, S. P. Spectrochim. Acta. Part A 2002, 58, 2759.
[106] Jiang, Z. L.; Sun, S. J.; Kang, C. Y. et al. Anal. Bioanal. Chem. 2005 381, 896
[107] 王丹,罗红群,李念兵等.西南师范大学学报(自然科学版),2004,29,1005.
[108] 刘绍璞,刘忠芳.分析化学,1996,24,665.
[109] Liu S. P.; Zhou G. M.; Liu Z. F. Fresenius J. Anal. Chem. 1999, 363, 651.
[110] Yang, C. X.; Li, Y. F.; Huang, C. Z. Anal. Bioanal. Chem. 2002, 374, 868.
[111] Huang, C. Z.; Li, Y. F.; Mao, J. G. et al. Analyst 1998, 123, 1401.
[112] Huang, C. Z.; Zhu, J. X.; Li, K. A. et al. Anal. Sci. 1997, 13, 263.
[113] Li, Y. F.; Huang, C. Z.; Li, M. Anal. Sci. 2002, 18, 177.
[114] Ma, C. Q.; Li, K. A.; Tong, S. Y. Analyst 1997, 122, 361.
[115] Ma, C. Q.; Li, K. A.; Tong, S.Y. Anal. Biochem. 1996, 239, 86.
[116] Ma, C. Q.; Li, K. A.; Tong, S. Y. Anal. Chim. Acta 1997, 338, 255.
[117] Ma, C. Q.; Li, K. A.; Tong, S. Y. Bull. Chem. Soc. Jpn 1997, 70, 129.
[118] Ma, C. Q.; Li, K. A.; Tong, S. Y. Fresenius J. Anal. Chem. 1997, 357, 915.
[119] Huang, C. Z.; Li, Y. F.; Feng, P. et al. Fresenius J. Anal. Chem., 2001, 371, 1034.
[120] Yang, C. X.; Li, Y. F.; Huang, C. Z. Anal. Lett. 2002, 35, 1945.
[121] 魏永巨,李克安,童沈阳.化学学报,1998,56,290.
[122] Fan, L.; Liu, S. P.; Yang, D. C. et al. Chin. J. Chem. 2003, 21, 56.
[123] 王小霞,沈含熙,郝永梅.分析化学,2000,28,1388.
[124] Guo, Z. X.; Shen, H. X. Anal. Chim. Acta 2000, 408, 177.
[125] Yao, G.; Li, K. A.; Tong, S. Y. Anal. Chim. Acta. 1999, 398, 319.
[126] Yao, G.; Li, K. A.; Tong, S. Y. Anal. Lett. 1998, 31, 1689.
[127] Li, Q. F.; Dong, L. J.; Jia, R. P. et al. Spectrosc. Lett. 2001, 34, 407.
[128] Jia, R. P.; Dong, L. J.; Li, Q. F. et al. Anal. Chim. Acta. 2001, 442, 249.
[129] Li, Q. F.; Zhang, H. Y.; Xue, C. X. et al. Spectrchim. Acta. A 2000, 56, 2465.
[130] Li, Q. F.; Chen, X. G.; Zhang, H. Y. et al. Fresenius J. Anal. Chem. 2000, 368, 715.
[131] Jia, R. P.; Dong, L. J.; Li, Q. F. et al. Talanta 2002, 57, 693.
[132] Dong, L. J.; Jia, R. P.; Li, Q. F. et al. Anal. Chim. Acta. 2002, 459, 313.
[133] Dong, L. J.; Jia, R. P.; Li, Q. F. et al. Fresenius J. Anal. Chem. 2001, 370, 1009.
[134] Dong, L. J., Jia, R. P., Li, Q. F. et al. Analyst 2001, 126, 707.
[135] Chen, Y. J.; Yang, J. H.; Wu, X. et al. Talanta 2002, 58, 869.
[136] Wu, H. L.; Li, W. Y.; He, X. W. Acta Chim. Sin. 2002, 60, 1822.
[137] 胡小莉,李胡芳,奉平.西南师范大学学报(自然科学版),1998,23,561.
[138] Cao, Q. E.; Ding, Z. T.; Fang, R. B. et al. Analyst 2001, 126, 1444.
[139] Ma, C. Q.; Li, K. A.; Tong, S. Y. Chin. Chem. Lett. 1997, 8, 57.
[140] Wang, Y. T.; Zhao, F. L.; Li, K. A. et al. Anal. Lett. 2000, 33, 221.
[141] Zhang, H.; Zhao, F. L.; Li, K. A. Anal. Lett. 2001, 34, 701.
[142] Yao, G.; Li, K. A.; Tong, S. Y. Talanta 1999, 50, 585.
[143] Li, S. W.; Li, N.; Zhao, F. L. et al. Spectrosc. Spect. Anal. 2002, 22, 619.
[144] Chen, L. H.; Zhao, F. L.; Li, K. A. Chin. J. Chem. 2002, 20, 368.
[145] Li, S. W.; Li, N.; Zhao, F. L. et al. Chin. J. Chem. Anal. 2002, 30, 732.
[146] Liu, R. T.; Yang, J. H.; Wu, X. et al. Spectrochim. Acta A 2002, 58, 3077.
[147] Cong, X.; Guo, Z. X.; Wang, X. X. et al. Anal. Chim. Acta 2001, 444, 205.
[148] 杨睿,刘绍璞,龙秀芬.西南师范大学学报(自然科学版),1999,24,179
[149] 范莉,刘绍璞,龙秀芬等.分析化学,2002,30,81.
[150] Liu, S. P.; Liu, Q. Anal. Sci. 2001, 17, 239.
[151] Liu, S. P.; Yang, R.; Liu, Q. Anal. Sci. 2001, 17, 243.
[152] Li, Q. F; Liu, S. H.; Zhang, H. Y. et al. Anal. Lett. 2001, 34, 1133.
[153] Wu, X.; Sun, S.; Guo, C. et al. Luminescence 2006, 21, 56.
[154] Huang, C. Z.; Yang, C. X.; Li, Y. F. Anal. Lett. 2003, 36, 1557.
[155] Li, Y. F.; Huang, C. Z; Huang, X. H. et al. Anal. Sci. 2000, 16, 1247.
[156] 龙秀芬,刘绍璞,西南师范大学学报(自然科学版),2000,25,155.
[157] Guo, Z. X.; Shen, H. X. Spectrochim. Acta. A 1999, 55 2919.
[158] Zhu, C. Q.; Li, D. H.; Zhu, Q. z. et al. Fresenius J. Anal. Chem. 2000, 366, 863.
[159] 江波,胡庆红.分析实验室,2002,21,27.
[160] Zhong, H.; Zhao, F. L.; Li, K. A. Anal. Lett. 2001, 34, 701.
[161] Feng, S.; Pan, Z.; Fan, J. Anal. Bioanal. Chem. 2005, 383, 255.
[162] Feng, S.; Pan, Z.; Fan, J. Spectrochim. Acta Part A 2006, 64, 574.
[163] Feng, P.; Hu, X. L.; Huang, C. Z. Anal. Lett. 1999, 32, 1323.
[164] Wang, Y. T.; Zhao, F. L.; Li, K. A. Fresenius J. Anal. Chem. 1999, 364, 560.
[165] Feng, N. C.; He, S. P.; Zhang, J. et al. Spectrosc. Spect. Anal. 2004, 24, 194.
[166] Liu, R.; Yang, J.; Sun, C. et al. Anal. Bioanal. Chem. 2003, 377, 375.
[167] Chert, Z.; Liu, J.; Liang, Y. et al. J. Biomol. Screen. 2006, 11, 400.
[168] Liu, Y.; Yang, J.; Liu, S. et al. Spectrochim. Acta Part A 2005, 61, 641.
[169] Zhong, H.; Wang, K.; Chen, H.-Y. Anal. Biochem. 2004, 330, 37.
[170] Zhao, G.-C.; Zhang, E; Wei, X.-W. et al. Anal. Biochem. 2004, 334, 297.
[171] Huang, C. Z.; Li, Y. F.; Liu, X. D. Anal. Chim. Acta 1998, 375, 89.
[172] 达毛拉·杰里力,黄承志.分析化学,1999,27,1204.
[173] 黄承志,李原芳,李念兵等.分析化学,1999,27,1241.
[174] Guo, Z. X.; Li, L.; Shen, H. X. et al. Anal. Chim. Acta 1999, 379, 45.
[175] 陈小兰,李东辉,朱庆枝等.高等学校化学学报,2001,22,901.
[176] Zhang, W. J.; Xu, H. P.; Wu, S. Q. et al. Analyst 2001, 126, 513.
[177] Liu, Y.; Ma, C. Q.; Li, K. A. et al. Anal. Chim. Acta 1999, 397, 39.
[178] Hao, Y. M.; Shen, H. X. Anal. Chim. Acta 2000, 413, 87.
[179] Hao, Y. M.; Shen, H. X. Anal. Chim. Acta 2000, 422, 159.
[180] Li, Y. F.; Huang, C. Z.; Huang, X. H. et al. Anal. Lett. 2001, 34, 1117.
[181] 黄新华,舒为群,李原芳等.分析化学,2001,29,271.
[182] Li, Z. P.; Li, K. A.; Tong, S. Y. Analyst, 1999, 124, 907.
[183] 朱昌青,李东辉,许金钩.分析化学,2000,28,1485.
[184] Huang, C. Z.; Li, Y. F.; Huang, X. H. et al. Analyst, 2000, 125, 1267.
[185] Liu, Y.; Ma, C. Q.; Li, K. A. et al. Anal. Biochem., 1999, 268, 187.
[186] Zhang, W. J.; Xu, H. P.; Xue, C. X. et al. Anal. Lett. 2001, 34, 553.
[187] 李天剑,沈含熙.高等学校化学学报,1998,19,1570.
[188] 刘晨,陈小明.分析化学,2001,29,685.
[189] 高峰,章丽,李永新等.分析科学学报,2005,21,623.
[190] Wang, Y. T.; Zhao, F. L.; Li, K. A. et al. Spectrochim. Acta, A, 2000, 56, 1827.
[191] Wang, Y. T.; Zhao, F. L.; Li, K. A. et al. Anal. Chim. Acta 1999, 396, 75.
[192] 黄新华,黄承志,黄玉明.西南师范大学学报(自然科学版),2000,25,273.
[193] Li, Y. F.; Huang, C. Z.; Huang, X. H. et al. Anal. Chim. Acta., 2001, 429, 311.
[194] Li, Y. F.; Huang, C. Z. Li, M. Anal. Chim. Acta 2002, 452, 285.
[195] 向海燕,陈小明,李松青等.分析化学,2000,28,1398.
[196] 杨传孝,李原芳,奉萍等.分析化学,2002,4,473.
[197] Li, Z. P.; Li, K. A.; Tong, S. Y. Talanta 2000, 51, 63.
[198] Li, Z. P.; Li, K. A.; Tong, S. Y. Talanta 2001, 55, 669.
[199] Liu, R. T.; Yang, J. H.; Wu, X. et al. Spectrochim. Acta, A, 2002, 58, 457.
[200] Liu, C.; Chen, X. T.; Li, S. Q. et al. Chin. J. Chem. Anal, 2002, 30, 1218.
[201] Du, X.; Sasaki, S.; Nakamura, H. et al. Talanta, 2001, 55, 93.
[202] Liu, R.T.; Yang, J. H.; Wu, X. et al. Analyst, 2001, 126, 1367.
[203] Wang, M.; Yang, J. H.; Wu, X. et al. Anal. Chim. Acta, 2000, 422, 151.
[204] Li, Y. F.; Shu, W. Q.; Feng, P. et al. Anal. Sci. 2001, 17, 693.
[205] Liu, R.T.; Yang, J. H.; Wu, X. et al. Anal. Chim. Acta 2001, 441, 303.
[206] 刘绍璞,胡小莉,罗红群等.中国科学(B)2002,32,18.
[207] Liu, R. T.; Yang, J. H.; Wu, X. et al. Anal. Chim. Acta 2001, 448, 85.
[208] Liu, R. T.; Yang, J. H.; Wu, X. Spectrochim. Acta, A, 2002, 58, 1935.
[209] Xiao, X. L.; Wang, Y. S.; Li, G. R. et al. Spectrosc. Spect. Anal. 2004, 24, 190.
[210] Chen, L.-H.; Nie, Y.-T.; Liu, L.-Z.; et al. Anal. Lett. 2003, 36, 107.
[211] Wu, X.; Wang, Y.; Wang, M. et al. Spectrochim. Acta Part A 2005, 61, 361.
[212] Cai, Z. X.; Song, G. W.; Li, L. et al. Chin. J. Anal. Chem. 2004, 32, 647.
[213] Jia, G.; Wang, P.; Qiu, J. et al. Anal. Lett. 2004, 37, 1339.
[214] Jie, N.; Hou, S.; Du, F. et al. Nucleos. Nucleot. Nucl. 2004, 23, 725.
[215] Chen, X.; Cai, C.; Luo, H. et al. Spectrochim. Acta Part A 2005, 61, 2215.
[216] Fang, F.; Zheng, H.; Li, L. et al. Spectrochim. Acta Part A 2006, 64, 698.
[217] Ding, F.; Zhao, H.; Chen, S. et al. Anal. Chim. Acta 2005, 536, 171.
[218] Wu, X.; Sun, S.; Yang, J. et al. Spectrochim. Acta Part A 2005, 62, 896.
[219] Jia, Z.; Yang, J.; Wu, X. et al. Spectrochim. Acta Part A 2006, 64, 555.
[220] Li, Y.; Zhu, C.; Wang, L. Microchim. Acta 2003, 142, 219.
[221] Li, Y.; Wu, Y.; Chen, J. et al. Anal. Bioanal. Chem. 2003, 377, 675.
[222] Gao, F.; Li, Y.-X.; Zhang, L. et al. Spectrochim. Acta Part A 2004, 60, 2505.
[223] Chen, X.; Cai, C.; Zeng, J. et al. Spectrochim. Acta Part A 2005, 61, 1783.
[224] Wang, F.; Yang, J.; Wu, X. et al. Spectrochim. Acta Part A 2006, in press.
[225] Jie, N.; Jia, G.; Hou, S. et al. Spectrochim. Acta PartA 2004, 59, 3295.
[226] 周颖琳,常文保,李元宗.分析化学,2004,32,169.
[227] Chen, Z. g.; Ding, W. f.; Ren, F. l. et al. Anal. Chim. Acta 2005, 550, 204.
[228] Song, G.; Cai, Z.; Li, L. Microchim. Acta 2004, 144, 23.
[229] Feng, S.; Liu, X.; Fan, J. Chin. J. Anal. Chem. 2005, 33, 377.
[230] Liu, S. P.; Luo, H. Q.; Li, N. B. et al. Anal. Chem. 2001, 73, 3907.
[231] Luo, H. Q.; Liu, S. P.; Li, N. B. et al. Anal. Chim. Acta. 2002, 468, 275.
[232] Zhang, S. Z.; Zhao, F. L.; Li, K. A. et al. Talanta 2001, 54, 333.
[233] Zhang, S. Z.; Zhao, F. L.; Li, K. A. et al. Anal. Chim. Acta. 2001, 431, 133.
[234] Liu, S. P.; Feng, P. Microchim. Acta. 2002, 140, 189.
[235] Fan, L.; Liu, S. P.; Yang, D. C. et al. Chin. J. Chem. 2002, 20, 1552.
[236] 胡小莉,刘绍璞,罗红群.化学学报,2003,61,1287.
[237] Hu, X. L.; Liu, S. P.; Li, N. B. Anal. Bioanal. Chem. 2003, 376, 42.
[238] Luo, H. Q.; Liu, S. P.; Liu, Z. F. et al. Anal. Chem. Acta 2001, 449, 261.
[239] Zhang, S. Z.; Li, N.; Zhao, F. L. et al. Spectrochim. Acta Part A 2002, 58, 273.
[240] Liu, S. P.; Zhang, Z. Y.; Luo, H. Q. et al. Anal. Sci. 2002, 18, 971.
[241] 刘绍璞,张筑元,胡小莉等.分析化学,2005,989.
[242] Liu, S. P.; Yang, Z.; Liu, Z. F. et al. Anal. Chim. Acta 2006, 572, 283.
[243] Liu, S. P.; Chen, Y. H.; Liu, Z. F. et al. Microchim. Acta 2006, 154, 87.
[244] Liu, S. P.; Luo, H. Q.; Xu, H. et al. Spectrochim. Acta Part A 2005, 61, 861.
[245] 杨明媚,蒋治良,光谱学与光谱分析,2002,22,180.
[246] 谢济运,蒋治良,钟福新等.分析测试技术与仪器,2000,6,178.
[247] 彭一兵,谢济运,蒋治良.广西科学,2001,8,197.
[248] 谢济运,蒋治良,物理化学学报,2001,17,406.
[249] 廖祖荷,罗杨合,蒋治良等.分析测试技术与仪器,2003,9,65.
[250] 陈绍芬,黄承志,谭克俊.高等学校化学学报,2006,27,1.
[251] Yguerabide, J.; Yguerabide, E. E. Anal. Biochem. 1998, 262, 137.
[252] Yguerabide, J.; Yguerabide, E. E. Anal. Biochem. 262 (1998) 157.
[253] He, Y. Q.; Liu, S. P.; Kong, L. et al. Spectrochim. Acta Part A 2005, 61, 2861.
[254] Zhu, J.; Huang, L.; Zhao, J. et al. Mat. Sci. Eng. B 2005, 121, 199.
[255] Zhu, J.; Wang, Y.; Huang, L. et al. Phys, Lett. A 2004, 323, 455.
[256] 蒋治良,西南师范大学博士学位论文 2003.
[257] 蒋治良,钟福新,李廷盛等.化学学报,2001,59,438.
[258] 蒋治良,冯忠伟,柳庆业等.无机化学学报,2001,17,355.
[259] 蒋治良,李芳,李廷盛等.高等学校化学学报,2000,21,1488.
[260] 蒋治良,分析测试技术与仪器,2000,6,200.
[261] Jiang, Z. L.; Feng, Z. W. Chin. Chem. Lett. 2001, 16, 556.
[262] 蒋治良,冯忠伟,李廷盛等.中国科学(B辑),2001,31,183.
[263] 蒋治良,光子学报,2001,30,460.
[264] 钟福新,蒋治良,李芳等.光谱学与光谱分析,2000,20,724.
[265] 蒋治良,刘绍璞,陈飒.贵金属,2001,22,8.
[266] 蒋治良,李芳,梁宏.化学学报,2001,8,93.
[267] Jiang, Z. L.; Liu, S. P.; Chen, S. Spectrochim. Acta Part A 2002, 58, 3121.
[268] 刘庆业,蒋治良,蒙冕武.分析测试技术与仪器,2001,7,199.
[269] 袁哲俊.纳米科学与技术 2005.
[270] 张立德,纳米材料 2000.
[271] Wu, L. P.; Li, Y. F.; Huang, C. Z. et al. Anal. Chem. 2006, 78, 5570.
[272] Shen, X. W.; Huang, C. Z.; Li, Y. F. Talanta in press.
[273] 代小霞,西南大学硕士论文 2006.
[274] Du, B.-A.; Li, Z.-P. Liu, C.-H. Angew. Chem. Int. Ed. 2006, 45, 8022.
[275] Jiang, Z. l.; Sun, S. j.; Liang, A. h. et al. Clin. Chem. 2006, 52, 1389.
[276] Asian, K.; Holley, P.; Davies, L. et al. J. Am. Chem. Soc. 2005, 127, 12115.
[277] Raman C. V.; Krishnan K. S. Nature 1928, 121, 501.
[278] Klinke, C.; Kurt, R.; Bonard, J.-M. J. Phys. Chem. B 2002, 106, 11191.
[279] Zhang, D. m.; Xie, Y.; Mrozek, M. F. et al. Anal. Chem. 2003, 75, 5703-5709
[280] Leites, L. A.; Bukalov, S. S.; Zabula, A. V. et al. J. Am. Chem. Soc. 2004, 126, 4114.
[281] Zhang, X. y.; Young, M. A.; Lyandres, O. et al. J. Am. Chem. Soc. 2005, 127, 4484.
[282] Shafer-Peltier, K. E.; Haynes, C. L.; Glucksberg, M. R. et aL J. Am. Chem. Soc. 2003, 125, 588.
[283] He, P.; Liu, H. t.; Li, Z. y. Langmuir 2004, 20, 10260.
[284] Nicolai, S. H. A.; Rubim, J. C. Langmuir 2003, 19, 4291.
[285] Stuart, D. A.; Yonzon, C. R.; Zhang, X. y. et al. Anal. Chem. 2005, 77, 4013.
[286] Alexander, B. D.; Dines, T. J. J. Phys. Chem. B 2005, 109, 3310.
[287] Cunningham, D.; Littleford, R. E.; Smith, W. E. Analyst 2005, 130, 472.
[288] McAnally, G.; McLaughlin, C.; Brown, R. et al. Analyst 2002, 127, 838.
[1] Yguerabide, J.; Yguerabide, E. E. Anal. Biochem. 1998, 262, 137.
[2] Yguerabide, J.; Yguerabide, E. E. Anal. Biochem. 1998, 262, 157.
[3] Lin, S.-Y.; Liu, S.-W.; Lin, C.-M. et al. Anal. Chem. 2002, 74, 330.
[4] Sato, K.; Hosokawa, K.; Maeda. M. J. Am. Chem. Soc, 2003, 125, 8102.
[5] Du, B.-A.; Li, Z.-P.; Liu. C.-H. Angew. Chem. Int. Ed. 2006, 45, 8022.
[6] Storhoff, J. J.; Lazarides, A. A.; Mucic, R. C. et al. J. Am. Chem. Soc. 2000, 122, 4640.
[7] Reynolds, Ⅲ, R. A.; Mirkin, C. A.; Letsinger, R. L. J. Am. Chem. Soc. 2000, 122, 3795.
[8] Elghanian, R.; Storhoff, J. J.; Mucic, R. C. et al. Science 1997, 277, 1078.
[9] Li, H.; Rothberg, L. Proc. Natl. Acad. Sci. 2004, 101, 14037.
[10] Storhoff, J. J.; Elghanian, R.; Mucic, R. C. et al. J. Am. Chem. Soc. 1998, 120, 1959.
[11] Mayes, A. G.; Blyth, J.; Millington, R. B. et al. Anal. Chem. 2002, 74, 3649.
[12] Kim, Y.; Johnson, R. C.; Hupp, J. T. Nano Lett. 2001, 1, 165.
[13] Liu, J. W.; Lu, Y. Anal. Chem. 2004, 76, 1627.
[14] Zhang, S.-B.; Wu, Z.-S.; Guo, M.-M. et al. Talanta 2007, 71, 1530.
[15] Lion, L. A.; Musick, M. D.; Natan, M. J. Anal. Chem. 1998, 70, 5177.
[16] Zhang, S.; Huang, F.; Liu, B. et al. Talanta 2007, 71, 874.
[17] Pavlov, V.; Xiao, Y.; Shlyahovsky, B. et al. J. Am. Chem. Soc. 2004, 126, 11768.
[18] Huang, C.-C.; Huang, Y.-F.; Cao, Z. et al. Anal. Chem. 2005, 77, 5735.
[19] Taton, T. A.; Lu, G.; Mirkin, C.A. J. Am. Chem. Soc. 2001, 123, 5164..
[20] Roll, D.; Malicka, J.; Gryczynski, I. et al. Anal. Chem. 2003, 75, 3440.
[21] Asian, K.; Lakowicz, J. R.; Geddes, C. D. Anal. Chem. 2005, 77, 2007.
[22] Asian, K.; Holley, P.; Davies, L. et al. J. Am. Chem. Soc. 2005, 127, 12115.
[23] Talley, C. E.; Jusinski, L.; Hollars, C. W. et al. Anal. Chem. 2004, 76, 7064.
[24] S. M. Nie, S. R. Emory. Science 1997, 275, 1102.
[25] Habuchi, S.; Cotlet, M.; Gronheid, R. et aL J. Am. Chem. Soc. 2003, 125, 8446.
[26] Kneipp, H.; Kneipp, J.; Kneipp, K. Anal. Chem. 2006, 78, 1363.
[27] Wu, L. P.; Li, Y. F.; Huang, C. Z. et al. Anal. Chem. 2006, 78, 5570.
[28] Fang, W. X.; Song, C. S.; Zhou. L. X. Chinese Tranditional Medicine Pharmacology, 1998, People's Medical Publishing House, Beijing, China.
[29] Ou, S. Y.; Bao, H. Y.; Lan. Z. D. J. Chin. Med. Mater. 2001, 24, 220.
[30] Cui, N.; Qian, J. H.; Qian, Y. Q. et al. Lishizhen Medicine and Material Medica Research. 2000, 11, 32.
[31] Li, X. P.; Yu, J.; Luo, J. Y. et al. Chem. Pharm. Bull. 2004, 52, 1251.
[32] Li, Y.; Biomed, K. Bi. Chromator. 2003, 17, 543.
[33] Lu, G.-H.; Chan, K.; Leung, K. et al. J. Chromatogr. A 2005, 1086, 209.
[34] Guo, T.; Sun, Y.; Sui, Y. et al. Anal. Bioanal. Chem. 2003, 375, 840.
[35] Ji, S. G.; Chai, Y. F.; Wu, Y. T. et al. Biomed. Chromatogr. 1999, 13, 333.
[36] Ai, Z.; Liao, F.; Zhu, L. et al. Chin. J. of Appl. Chem. 2006, 123, 566.
[37] Cao, W.; Suo, Z. R.; Song, J. R. et al. J. Chin. Univ. 2005, 26, 1424.
[38] Li, L. N.; Li, N. B.; Luo, H. Q. Anal. Sci. 2005, 21, 963.
[39] 谭克俊,西南大学博士论文,2006.
[40] Tokareva, I.; Hutter, E. J. Am. Chem. Soc. 2004, 126, 15784.
[41] Asian, K.; Holley, P.; Davies, L. et aI. J. Am. Chem. Soc. 2005, 127, 12115.
[42] Selvakannan, P. R.; Swami, A.; Srisathiyanarayanan, D. Langrnuir 2004, 20, 7825.
[43] Scampicchio, M.; Wang, J.; Blasco, A. J. et al. Anal. Chem. 2006, 78, 2060.
[44] Baron, R.; Zayats, M.; Willner. I. Anal. Chem. 2005, 77, 1566.
[45] Z.L柯鲁克,C.,J.皮科克[英]著,岳旺,张士善译,河北医学院出版,1984年,p34.
[46] 陈新谦,金有豫 主编.新编药物北京:人民卫生出版社,2000年,p290-294.
[47] 董宝平,化学教育,2004,6,8.
[48] El-Kommos, M. E.; Mohamed, F. A.; Khedr, A. S. Talanta 1990, 37, 625.
[49] 龙云,李东辉,冯建章等.分析化学,1997,25,916.
[50] Solich, P.; Polydorou, C. K.; Koupparis, M. A. et al. J. Pharm. Biomed. Anal. 2000, 22, 781.
[51] Klegeris, A.; Korkina, L. G.; Greenfield, S. A. Free Radical Biol. Med. 1995, 18, 215.
[52] Baron, R.; Zayats, M.; Willner. I. Anal. Chem. 2005, 77, 1566.
[53] 童裳伦,朱岩,杨景和.分析化学,2000,28,293.
[54] 吴惠毅,沈来龙,陈晋等.临床检验杂志,1997,1,13.
[55] Wang, H. Y.; Sun, Y.; Tang, B. Talanta 2002, 57, 899.
[56] 李丽清,孟兆坷,吕九如,泰山医学学报 1998,19,115.
[57] 胡玉斐,吕弋,何德勇等.分析实验室,2004,23,18.
[58] Zhang C.; Huang J.; Zhang Z. Anal. Chim. Acta. 1998, 374, 105.
[59] Zhang, L.; Teshima, N.; Hasebe, T.; et al. Talanta 1999, 50, 677.
[60] Zhou, G.; Zhang, G.; Chen, H. Anal. Chim. Acta. 2002, 463, 257.
[61] Michalowski, J.; Halaburda, P. Talanta 2001, 55, 1165.
[62] Li, B.; Zhang, Z.; Jin, Y. Biosens. Bioelectron. 2002, 17, 585.
[63] 何德勇,吕弋,胡玉斐等.分析化学,2003,31,1247.
[64] 何树华,吕弋,何德勇等.西南师范大学学报(自然科学版),2003,28,802.
[65] 何德勇,吕弋,胡玉斐等.西南师范大学学报(自然科学版),2003,28,334.
[66] 张迎雪,陈福南,章竹君.分析化学,2004,32,769.
[67] Ragab, G. H.; Nohta, H.; Zaitsu, K. Anal. Chim. Acta. 2000, 403, 155.
[68] Fotopoulou, M. A.; Ioannou, P. C. Anal. Chim. Acta. 2002, 462, 179.
[69] Virag, L.; Whittington, R. A. J. Chromatogr B 2002, 772, 267.
[70] Hansen, A. M.; Kristiansen, J.; Nielsen, J. L. et al. Talanta 1999, 50, 367.
[71] 陈福南,张迎雪,章竹君等.分析化学,2005,33,1771.
[72] Swanek, F. D.; Chen, G.; Ewing, A. G. Anal. Chem. 1996, 68, 3912.
[73] Tsai, H.-c.; Whang, C.-w. Electrophoresis 1999, 20, 2533.
[74] Britz-Mckibbin, P.; Wong, J.; Chen, D. Y. J. Chromatogr. A 1999, 853, 535.
[75] Peterson, Z. D.; Collins, D. C.; Bowerbank, C. R. et al. J. Chromatogr B, 2002, 776, 221.
[76] 张东明,付敏,马万云等.分析科学学报,2002,18,265.
[77] Kawagoe, K. T.; Zimmerman, J. B.; Wightman, R. M. J. Neurosci. Methods 1993, 48, 225.
[78] Kharitonov, A. B.; Shipway, A. N.; Willner, Ⅰ. Anal. Chem. 1999, 71, 5441.
[79] Chen, R.-S.; Huang, W.-H.; Tong, H. et al. Anal. Chem. 2003, 75, 6341.
[80] Raj, C. R.; Okajima, T.; Ohsaka, T. J. Electroanal. Chem. 2002, 543, 127.
[81] Liu, T.; Li, M.; Li, Q. Talanta 2004, 63, 1053.
[82] Raj, C. R.; Ohsaka, T. J. Electroanal. Chem. 2001, 496, 44.
[83] Ten Cate, M. G. J.; Crego-Calama, M.; Reinhoudt, D. N. J. Am. Chem. Soc. 2004, 126, 10840.
[84] Pashley, R. M. J. Phys. Chem. B 2003, 107, 1714.
[85] Shen, X.-M.; Dryhurst, G. Bioorg. Chem. 1996, 24, 340.
[86] 何德勇,西南师范大学硕士论文,2003.
[87] Ragabl, G. H.; Nohta, H.; Zaitsu K. Anal. Chim. Acta. 2000, 403, 155.
[88] Arriagada, C.; Paris, Ⅰ.; Sanchez de las Matas, M. J. et al. J. Neurobiol. Dis. 2004, 16, 468.
[89] Caswell, K. K.; Bender, C. M.; Murphy, C. J. Nano. Lett. 2003, 5, 667.
[90] Joyce, G. F. Gene 1989, 82, 83.
[91] Robertson, D. L.; Joyce, G. E. Nature 1990, 344, 467.
[92] Ellington, A. D.; Szostak, J. W. Nature 1990, 346, 818.
[93] Tuerk, C.; Gold, L. Science 1990, 249, 505.
[94] Bock, L. C.; Griffin, L. C.; Latham, J. A. et al. Nature 1992, 355.
[95] Potyrailo, R. A.; Conrad, R. C.; Ellington, A. D. Anal. Chem. 1998, 70, 3419.
[96] German, I.; Buchanan, D.; Kennedy, R. T. Anal. Chem. 1998, 70, 4540.
[97] Charlton, J.; Kirschenheuter, G. P.; Smith, D. Biochemistory 1997, 36, 3018.
[98] Fang, X.; Cao, Z.; Beck, T. et al. Anal. Chem. 2001, 73, 5752.
[99] Jiang, Y.; Fang, X.; Bai, C. Anal. Chem. 2004, 76, 5230.
[100] Sassanfar, M.; Szostak, J. W. Nature 1993, 364, 550.
[101] Deng, Q.; German, I.; Buchanan, D. et al. Anal. Chem. 2001, 73, 5415.
[102] Kleinjung, F.; Klussman, S.; Erdmann, V. A. et al. Anal. Chem. 1998, 70, 328.
[103] Jenison, R. D.; Gill, S. C.; Pardi, A. et al. Science 1994, 263, 1425.
[104] Stojanovic, M. N.; Landry, D. W. J. Am. Chem. Soc. 2001, 123, 4928.
[105] Stojanovic, M. N.; Landry, D. W. J. Am. Chem. Soc. 2002, 124, 9678.
[106] Burke, D. H.; Hoffman, D. C.; Brown, A. et al. Chem. Biol. 1997, 4, 833.
[107] Famulok. M. J. Am. Chem. Soc. 1994, 116, 1698.
[108] Baldrich, E.; Acero, J. L.; Reekrnans, G. et al. Anal. Chem. 2005, 77, 4774.
[109] Stadtherr, K.; Wolf, H.; Lindner, P. Anal. Chem. 2005, 77, 3437.
[110] Stojanovic, M. N.; Prada, P.; Landry, D. W. J. Am. Chem. Soc. 2000, 122, 11547.
[111] Wang, J.; Jiang, Y.; Zhou, C. et al. Anal. Chem. 2005, 77, 3542.
[112] Koizumi, M.; Breaker, R. R. Biochemistry 2000, 39, 8983.
[113] Fahlman, R. P.; Sen, D. J. Am. Chem. Soc. 2002, 124, 4610.
[114] Anderson, P. C.; Sandro Mecozzi, S. J. Am. Chem. Soc. 2005, 127, 5290.
[115] Stojanovic, M.N.; Landry, D. W. J. Am. Chem. Soc. 2002, 124, 9678.
[116] Baker, B. R.; Lai, R. Y.; Wood, M. S. et al. J. Am. Chem. Soc. 2006, 128, 3138.
[117] Heyduk, E.; Heyduk, T. Anal. Chem. 2005, 77, 1147.
[118] Radi, A.-E.; Llul's Acero Sa' nchez, J.; Baldrich, E. et al. Anal. Chem. 2005, 77, 6320.
[119] Polsky, R.; Gill, R.; Kaganovsky, L. et al. Anal. Chem. 2006, 78, 2268.
[120] Floch, F. L.; Ho, H. A.; Leclerc, M. Anal. Chem. 2006, 78, 4727.
[121] So, H.-M.; Won, K.; Kim, Y. H. et al. J. Am. Chem. Soc. 2005, 127, 11906.
[122] Radi, A.-E.; Lluis Acero Sa'nchez, J.; Baldrich, E. et al. J. Am. Chem. Soc. 2006, 128, 117.
[123] Xiao, Y.; Piorek, B. D.; Plaxco, K. W. et al. J. Am. Chem. Soc. 2005, 127, 17990.
[124] Choi, J. H.; Chen, K. H.; Strano, M. S. J. Am. Chem. Soc. 2006, 128, 15584.
[125] Basnar, B.; Elnathan, R.; Willner, I. Anal. Chem. 2006, 78, 3638.
[126] Potyrailo, R. A.; Conrad, R. C.; Ellington, A. D. et al. Anal. Chem. 1998, 70, 3419.
[127] Singh, R. R.; Chang, J. Y. Biochimica & Biophysica Acta 2003, 1651, 85.
[128] Frens, G. Nat. Phys. Sci. 1973, 241, 20.
[129] Grabar, K. C.; Freeman, R. G.; Hommer, M. B. et al. J. Anal. Chem. 1995, 67, 735.
[130] Mucic R. C.; Storhoff J. J.; Mirkin C. A. et al. J. Am. Chem. Soc., 1998, 120, 12674.
[131] Storhoff J. J., Lazarides A. A., Robert C. M. et al. J. Am. Chem. Soc., 2000, 122, 4640.
[132] Maye, M. M.; Nykypanchuk, D.; Lelie, D. et al. J. Am. Chem. Soc. 2006, 128, 14020.
[133] Li, J. J.; Fang, X.; Tan, W. Biochem. Biophys. Res. Comm. 2002, 292, 31.
[1] Cunningham, D.; Littleford, R.. E.; Smith. W. E. et al. Analyst 2005, 130.472.
[2] Yuan, P.; Walt, D. R. Anal. Chem. 1987, 59, 2391.
[3] Holland, J. F.; Teets, R. E.; Kelly, P. M. et al. Anal. Chem. 1977, 49, 706.
[4] MacDonalda, B. C.; Lvinb, S. J.; Pattersonc, H. Anal. Chim. Acta. 1997, 338, 155.
[5] Parker, C. A.; Barnes, W. J. Analyst 1957, 82, 606.
[6] Fanget, B.; Devos, O.; Draye, M. Anal. Chem. 2003, 75, 2790.
[7] Aarnoutse, P. J.; Westerhuis, J. A. Anal. Chem. 2005, 77, 1228.
[8] Shao, N.; Zhang, Y.; Cheung, S. M. et al. Anal. Chem. 2005, 77, 7294.
[9] Yang, X. H.; Wang, K. M.; Guo, C. C. Anal. Chim. Acta 2000, 407, 45.
[10] He, H. R.; Li, H.; Mohr, G. et al. Anal. Chem. 1993, 65, 123.
[11] Zhong, J. R.; Liu, M. G.; Ao, B. Y. et al. Chem. J. Chin. Univ. 2001, 22, 191.
[12] Gabor, G.; Walt, D. R. Anal. Chem. 1991, 63, 793.
[13] Tohda, K.; Lu, H. W.; Umezawa, Y. et al. Anal. Chem. 2001, 73, 2070.
[14] Ohno, T. Environ. Sci. Technol. 2002, 36, 742.
[15] Chen, G. Z.; Huang, X. Z.; Zheng, Z. Z. et al. Spectrofluorometry; Science press: Beijing, 1990, p.104.
[16] Cheng, K. L.; Ueno, K.; Imamura, T. CRC Handbook of Organic Analytical Reagents (Chinese Version); Geology Press: Beijing, 1982, p. 142.
[17] 黄承志,北京大学博士学位论文,1996.
[18] Shozo, S.; Masamichi, F.; Yoshio, I. et al. Anal. Chim. Acta 1971, 55, 231.
[1] Moody, C. A.; Kwan, W. C.; Martin, J. W. et al. Anal. Chem. 2001, 73, 2200.
[2] Ren, J. c.; Ulvik, A.; Refsum, H. Anal. Chem. 2002, 74, 295.
[3] Suhara, Y.; Kamao, M.; Tsugawa, N. et al. Anal. Chem. 2005, 77, 757.
[4] Song, L. g.; James, S. R.; Kazim, L. et al. Anal. Chem. 2005, 77, 504.
[5] Lu, C.; Zu, Y. b.; Yam, V. W.-W. Anal. Chem. 2007, 79, 666.
[6] 陈义,齐莉,韩志强,余晓.广西师范大学学报(自然科学版),2003,21,10.
[7] Qi, L., Hart, Z. Q., Chen, Y. J. Chromatogr. A 2006, 1110, 235.
[8] Reynolds, Ⅲ, R. A.; Mirkin, C. A.; Letsinger, R. L. J. Am. Chem. Soc. 2000, 122, 3795.
[9] Elghanian, R.; Storhoff, J. J.; Mucic, R. C. et al. Science 1997, 277, 1078.
[10] 白泉,孔宇,耿信笃.高等化学学报,2002,23,1483.
[11] 董方霆,廖杰,蔡耘等.色谱,1997,15,420.
[12] Toriumi, C.; Imai, K. Anal. Chem. 2002, 74, 2321.
[13] Roll, D.; Malicka, J.; Gryczynski, I. et al. Anal. Chem. 2003, 75, 3440.
[14] Asian, K.; Lakowicz, J. R.; Geddes, C. D. Anal. Chem. 2005, 77, 2007.