欧洲葡萄‘无核白’胚珠ESTs分析与EF-hand钙结合蛋白基因的鉴定
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摘要
本研究在前期构建的欧洲葡萄‘无核白’胚珠cDNA文库的基础上,通过噬菌体侵染宿主菌XL1-Blue筛选阳性克隆后,以大肠杆菌BM25.8宿主菌进行培养,实现阳性克隆转换,对阳性克隆进行测序获得EST序列,并对特异序列进行鉴定,取得的主要结果如下:
1.从欧洲葡萄‘无核白’胚珠cDNA文库中随机挑选了4800个克隆进行5’端测序,经剔除载体、污染序列和小于100 bp的序列后,共获得了3908条EST,这些序列登陆了NCBI GenBank数据库,这些序列及登陆号列入附录。
2.对3908条EST聚类分析获得了3046个独立序列。这些独立序列对比拟南芥功能分类标准,共有2056个独立序列分为21类。其中与新陈代谢有关的有435个独立序列,能量有关的22个独立序列,储藏蛋白有关的6个,细胞周期和DNA加工有关的82个,翻译有关的110个,蛋白质合成有关的138个,蛋白质命运(折叠、修复、靶向运输)有关的183个,结合或辅助功能蛋白(结构的或催化的)有关的279个,新陈代谢调控和蛋白调控有关的11个,细胞运输、运输功能和运输路径有关的101个,细胞交谈/信号转导机制有关的10个,细胞自救、防御和抗病有关的39个,与环境间的相互作用有关的21个,系统与环境间的相互作用有关的1个,细胞命运有关的6个,系统发育有关的15个,细胞组分的生物进化有关的3个,器官分化有关的1个,亚细胞定位有关的158个,不明确的分类有关的126个,未获得分类的309个。
3.进一步对获得的3046个独立序列与NCBI GenBank nr蛋白质数据库进行同源比对(E-value≤1e-5),共获得了69个与种子或胚发育相关的蛋白基因的独立序列,其中涉及与未受精胚囊蛋白基因(Unfertilized embryo sac)有关的独立序列3个,体细胞胚胎发生相关蛋白基因(Somatic embryogenesis related protein genes)有关的独立序列15个,胚发育缺陷蛋白基因(Embryo Defective genes)有关的独立序列17个,胚囊发育停止蛋白基因( Embryo sac development arrest genes)有关的独立序列7个,细胞程序性死亡(death-related protein gene)相关基因有关的独立序列6个,钙结合蛋白(Calcium-binding protein gene)相关基因有关的独立序列21个。
4.从获得与细胞程序性死亡和钙结合蛋白相关的27个独立序列中分离了一个序列,该序列在无核与有核葡萄胚珠发育过程中表达差异显著。通过Race技术扩增,获得该序列全长为907 bp(登陆GenBank accession no. HQ214155),拥有2个EF-hand钙结合蛋白模体,简称之为VvCBP1基因,该基因序列与番茄同源基因的同源性为100%。利用VIGS技术在番茄中沉默VvCBP1的同源基因以间接验证VvCBP1的功能。研究结果表明,瞬时沉默番茄果实中种子数量明显减少。这个结果暗示,VvCBP1对番茄种子发育进程中有促使幼胚败育作用。
This study was conducted on the basis of the cDNA library previously constructed from ovules of Vitis vinifera cv. Thompson Seedless. Positive clones were screened after bacteriophage infection of XL1-Blue and further culture with BM25.8 as host bacteria. The obtained positive clones were selected for sequencing and the isolated specific sequences were further identified. The main results are described as follows.
1. 4800 clones from the cDNA library of ovules of V. vinifera cv.Thompson Seedless were randomly selected for 5’end sequencing. After removing vector sequences, polluted sequences and low quality sequences less than 100 bases, a total of 3908 high quality ESTs were obtained and have been deposited in NCBI GenBank database with accession number(Appendix).
2. The 3908 ESTs were assembled into 3046 unique sequences. These unique sequences were aligned with the functional classification system of Arabidiopsis thaliana, and 2056 unique ESTs could be classified into 21 categories. Among them, the number of classified ESTs associated with metabolism, Energy, Storage protein, Cell cycle and DNA Processing, transcription, Protein synthesis, Protein fate(folding, modification, destination), Protein with binding function or cofactor requirement, Regulation of metabolism and protein function, Cellular transport, Transport facilitation and transport routes, Cellular communication/ signal transduction mechanism, Interaction with the environment, Systemic interaction with the environment, Cell fate, Development, Biogenesis of cellular components, Organ differentiation and Subcellular localization, were 435, 22, 6, 82,110, 138,183, 279, 11, 101, 10, 21, 1, 6,15,3, 1 and 158,respectively. 126 ESTs could not fell into the well-defined classification and 309 ESTs could not be were unclassified.
3. The 3046 ESTs were further analyzed and 69 unique ESTs involving in seed or embryo development were obtained using the BLASTX against the NCBI non-redundant protein database (E-value≤1e-5). Among them, the number of ESTs involved in unfertilized embryo sac, Somatic embryogenesis related protein, Embryo Defective, Embryo sac development arrest genes, Cell death-related protein, and Calcium-binding protein, were 3, 15, 17, 7, Cell death-related protein 6, and 21, respectively.
4. A novel gene was found from 27 unigenes involving in Cell death-related protein genes and Calcium-binding protein, and its expression pattern in embryo is significantly different between seedless and seeded grapevine species. Based on the obtained partial EST sequence, a RACE-PCR method was performed to isolate the full-length gene. The result indicated that the gene is 907 bp in length, and possesses two EF-hand Calcium-binding motifs, and designated VvCBP1, which shares high similarities with that of the tomato. Using tobacco rattle virus (TRV)-mediated virus-induced gene silencing (VIGS) technology, we silenced the tomato homolog of VvCBP1 in tomato fruits, which led to the number of injected tomato seed significantly decrease. These results suggest that VvCBP1 may be associated with fruit seedlessness of tomato.
1.从欧洲葡萄‘无核白’胚珠cDNA文库中随机挑选了4800个克隆进行5’端测序,经剔除载体、污染序列和小于100 bp的序列后,共获得了3908条EST,这些序列登陆了NCBI GenBank数据库,这些序列及登陆号列入附录。
2.对3908条EST聚类分析获得了3046个独立序列。这些独立序列对比拟南芥功能分类标准,共有2056个独立序列分为21类。其中与新陈代谢有关的有435个独立序列,能量有关的22个独立序列,储藏蛋白有关的6个,细胞周期和DNA加工有关的82个,翻译有关的110个,蛋白质合成有关的138个,蛋白质命运(折叠、修复、靶向运输)有关的183个,结合或辅助功能蛋白(结构的或催化的)有关的279个,新陈代谢调控和蛋白调控有关的11个,细胞运输、运输功能和运输路径有关的101个,细胞交谈/信号转导机制有关的10个,细胞自救、防御和抗病有关的39个,与环境间的相互作用有关的21个,系统与环境间的相互作用有关的1个,细胞命运有关的6个,系统发育有关的15个,细胞组分的生物进化有关的3个,器官分化有关的1个,亚细胞定位有关的158个,不明确的分类有关的126个,未获得分类的309个。
3.进一步对获得的3046个独立序列与NCBI GenBank nr蛋白质数据库进行同源比对(E-value≤1e-5),共获得了69个与种子或胚发育相关的蛋白基因的独立序列,其中涉及与未受精胚囊蛋白基因(Unfertilized embryo sac)有关的独立序列3个,体细胞胚胎发生相关蛋白基因(Somatic embryogenesis related protein genes)有关的独立序列15个,胚发育缺陷蛋白基因(Embryo Defective genes)有关的独立序列17个,胚囊发育停止蛋白基因( Embryo sac development arrest genes)有关的独立序列7个,细胞程序性死亡(death-related protein gene)相关基因有关的独立序列6个,钙结合蛋白(Calcium-binding protein gene)相关基因有关的独立序列21个。
4.从获得与细胞程序性死亡和钙结合蛋白相关的27个独立序列中分离了一个序列,该序列在无核与有核葡萄胚珠发育过程中表达差异显著。通过Race技术扩增,获得该序列全长为907 bp(登陆GenBank accession no. HQ214155),拥有2个EF-hand钙结合蛋白模体,简称之为VvCBP1基因,该基因序列与番茄同源基因的同源性为100%。利用VIGS技术在番茄中沉默VvCBP1的同源基因以间接验证VvCBP1的功能。研究结果表明,瞬时沉默番茄果实中种子数量明显减少。这个结果暗示,VvCBP1对番茄种子发育进程中有促使幼胚败育作用。
This study was conducted on the basis of the cDNA library previously constructed from ovules of Vitis vinifera cv. Thompson Seedless. Positive clones were screened after bacteriophage infection of XL1-Blue and further culture with BM25.8 as host bacteria. The obtained positive clones were selected for sequencing and the isolated specific sequences were further identified. The main results are described as follows.
1. 4800 clones from the cDNA library of ovules of V. vinifera cv.Thompson Seedless were randomly selected for 5’end sequencing. After removing vector sequences, polluted sequences and low quality sequences less than 100 bases, a total of 3908 high quality ESTs were obtained and have been deposited in NCBI GenBank database with accession number(Appendix).
2. The 3908 ESTs were assembled into 3046 unique sequences. These unique sequences were aligned with the functional classification system of Arabidiopsis thaliana, and 2056 unique ESTs could be classified into 21 categories. Among them, the number of classified ESTs associated with metabolism, Energy, Storage protein, Cell cycle and DNA Processing, transcription, Protein synthesis, Protein fate(folding, modification, destination), Protein with binding function or cofactor requirement, Regulation of metabolism and protein function, Cellular transport, Transport facilitation and transport routes, Cellular communication/ signal transduction mechanism, Interaction with the environment, Systemic interaction with the environment, Cell fate, Development, Biogenesis of cellular components, Organ differentiation and Subcellular localization, were 435, 22, 6, 82,110, 138,183, 279, 11, 101, 10, 21, 1, 6,15,3, 1 and 158,respectively. 126 ESTs could not fell into the well-defined classification and 309 ESTs could not be were unclassified.
3. The 3046 ESTs were further analyzed and 69 unique ESTs involving in seed or embryo development were obtained using the BLASTX against the NCBI non-redundant protein database (E-value≤1e-5). Among them, the number of ESTs involved in unfertilized embryo sac, Somatic embryogenesis related protein, Embryo Defective, Embryo sac development arrest genes, Cell death-related protein, and Calcium-binding protein, were 3, 15, 17, 7, Cell death-related protein 6, and 21, respectively.
4. A novel gene was found from 27 unigenes involving in Cell death-related protein genes and Calcium-binding protein, and its expression pattern in embryo is significantly different between seedless and seeded grapevine species. Based on the obtained partial EST sequence, a RACE-PCR method was performed to isolate the full-length gene. The result indicated that the gene is 907 bp in length, and possesses two EF-hand Calcium-binding motifs, and designated VvCBP1, which shares high similarities with that of the tomato. Using tobacco rattle virus (TRV)-mediated virus-induced gene silencing (VIGS) technology, we silenced the tomato homolog of VvCBP1 in tomato fruits, which led to the number of injected tomato seed significantly decrease. These results suggest that VvCBP1 may be associated with fruit seedlessness of tomato.
引文
刘家驹,卢春生.1985.无核葡萄.果树科学,4:15-20
蒋迪军.1985.无核白等几个葡萄品种的细胞学研究.果树科学,21(2):10-21
孙亮先等.2002.水稻胚乳及幼苗基因表达谱的研究.[博士论文].杭州.浙江大学
王近卫,堀内昭作,林伯年等.1992.无核白葡萄的无核果形成的组织形态学研究.园艺学报,19(1):1-6
杨英军,王跃进,周鹏,等.2002.葡萄无核基因SCAR标记的序列构成与酶切分析.果树学报, 19(1):12-14
杨克强,王跃进,张今今,王西平,万怡震,张剑.2005.葡萄无核基因定位与作图的研究.遗传学报, 32(3):297-302
张宏明,孟新法,张潞生.1990.无核葡萄胚珠发育及早期离体培养的研究Ⅰ.激素对胚珠离体培养的影响.北京农业大学学报,16(3):277-282
张朝红,王跃进,王西平,杨亚州.2007.‘杨格尔’与‘京秀’葡萄胚珠发育过程中的活性氧代谢比较.园艺学报,34(6):1361-1366
张朝红,王跃进.2007.无核葡萄胚珠发育进程中EST的分析与败育相关基因的克隆.[博士论文].杨凌.西北农林科技大学
王飞,王跃进,周会玲,万怡震,杨进孝.2005.无核葡萄与中国野生葡萄杂种胚发育和败育的细胞学研究.西北农林科技大学(自然科学版),33(3):61-65
刘小宁,王跃进,张剑侠,江淑平.2005.Flame Seedless葡萄胚珠、胚乳及胚发育与败育的研究.西北植物学,25(10):1947-1953
Aaronson J S, Eckman B, Blevins R A, Borkowski J A, Myerson J, Imran S, Elliston K O. 1996. Toward the development of a gene index to the human genome: An assessment of the nature of high-throughput EST sequence data. Genome Research, 6(9):829-845
Ablett E, Seaton G, Scott K, Shelton D, Graham M W, Baverstock P, Lee L S, Henry R. 2000. Analysis of grape ESTs: global gene expression patterns in leaf and berry. Plant Science, 159(1):87-95
Adams M D, Dubnick M, Kerlavage a R, Moreno R, Kelley J M, Utterback T R, Nagle J W, Fields C, Venter J C. 1992. Sequence identification of 2,375 human brain genes. Nature, 355(6361):632-634
Adams M, Kelley J, Gocayne J, Dubnick M, Polymeropoulos M, Xiao H, Merril C, Wu A, Olde B, Moreno R, Et A. 1991. Complementary DNA sequencing: expressed sequence tags and human genome project. Science, 252(5013):1651-1656
Agarwal P, Arora R, Ray S, Singh A, Singh V, Takatsuji H, Kapoor S, Tyagi A. 2007. Genome-wide identification of C<sub>2</sub>H<sub>2</sub> zinc-finger gene family in rice and their phylogeny and expression analysis. Plant Molecular Biology, 65(4):467-485
Bishop J O, Morton J G, Rosbash M, Richardson M. 1974. Three abundance classes in HeLa cell messenger RNA. Nature, 250(463):199-204
Bouquet A, Danglot Y. 1996. Inheritance of seedlessness in grapevine (Vitis vinifera L). Vitis, 35(1):35-42
Cabezas J A, Cervera M T, Ruiz G, A L, Carre, O J, Mart, Nez-Zapater J M. 2006. A genetic analysis of seed and berry weight in grapevine. Genome, 49(12):1572-1585
Cakir B, Agasse A, Gaillard C, Saumonneau A, Delrot S, R A. 2003. A Grape ASR Protein Involved inSugar and Abscisic Acid Signaling. The Plant Cell Online, 15(9):2165-2180
Chatthai M, Kaukinen K H, Tranbarger T J, Gupta P K, Misra S. 1997. The isolation of a novel metallothionein-related cDNA expressed in somatic and zygotic embryos of Douglas-fir: regulation by ABA, osmoticum, and metal ions. Plant Molecular Biology, 34(2):243-254
Ciftci-Yilmaz S, Mittler R. 2008. The zinc finger network of plants. Cellular and Molecular Life Sciences, 65(7):1150-1160
Colova-Tsolova V, Jiang L, Perl A. 2003. Cyto-embryological aspects of seedlessness in Vitis vinifera L. and exploiting DNA recombinant technology as an advanced approach for introducing seedlessness into vinifera and muscadine grapes. Acta Hort, 603(1):195-199
da Silva, F.G, et al. 2005. Characterizing the grape transcriptome. Analysis of expressed sequence tags from multiple Vitis species and development of a compendium of gene expression during berry development. Plant Physiol, 139:574-597
Danielle Costenaro-da-Silvaa, GiselePassaiab, Jo?oA.P. Henriquesa, RogérioMargisa, Giancarlo Pasquali, LuísF.Revers. 2010. Identification and expression analysis of genes associated with the early berry development in the seedless grapevine (Vitis vinifera L.) cultivar Sultanine.Plant Science,179(5): 510-519
Degtyarenko K N, Archakov a I. 1993. Molecular evolution of P450 superfamily and P450-containing monooxygenase systems. FEBS Letters, 332(1-2):1-8
Delorme V G R, Mccabe P F, Kim D-J, Leaver C J. 2000. A Matrix Metalloproteinase Gene Is Expressed at the Boundary of Senescence and Programmed Cell Death in Cucumber. Plant Physiology, 123(3):917-928
Doligez a D, Bouquet a B, Danglot Y D, Lahogue F L, Riaz S R, Meredith C M, Edwards K E, This P T. 2002. Genetic mapping of grapevine (Vitis vinifera L.) applied to the detection of QTLs for seedless and berry weight. TAG Theoretical and Applied Genetics, 105(5):780-795
Stekel D J, Git Y, Falciani F. 2000. The Comparison of Gene Expression from Multiple cDNA Libraries. Genome Research, 10(12):2055-2061
Dure L, Crouch M, Harada J, Ho T-H D, Mundy J, Quatrano R, Thomas T, Sung Z R. 1989. Common amino acid sequence domains among the LEA proteins of higher plants. Plant Molecular Biology, 12(5):475-486
Ehlting J, Sauveplane V, Olry A, Ginglinger J F, Provart N, Werck-Reichhart D. 2008. An extensive (co-) expression analysis tool for the cytochrome P450 superfamily in Arabidopsis thaliana. BMC Plant Biology, 8(1):47
Ewing B, Green P. 1998. Base-Calling of Automated Sequencer Traces UsingPhred.II. Error probabilities. Genome Research, 8(3):186-194
Ewing B, Hillier L, Wendl M C, Green P. 1998. Base-calling of automated sequencer traces using phred. Genome Research, 8(3):175-185
Ewing R M, Kahla a B, Poirot O, Lopez F, Audic S, Claverie J-M. 1999. Large-Scale Statistical Analyses of Rice ESTs Reveal Correlated Patterns of Gene Expression. Genome Research, 9(10):950-959
Fred Y. Peng , Karen E. Reid , Nancy Liao , James Schlosser , Diego Lijavetzky , Robert Holt ,JoséM. Martínez Zapater , Steven Jones , Marco Marra , J?rg Bohlmann , Steven T. Lund. 2007. Generation of ESTs in Vitis vinifera wine grape (Cabernet Sauvignon) and table grape (Muscat Hamburg) anddiscovery of new candidate genes with potential roles in berry development. Gene, 402(1-2):40-50
Frey A, Godin B, Bonnet M, Sotta B, Marion-Poll A. 2004. Maternal synthesis of abscisic acid controls seed development and yield in <i>Nicotiana plumbaginifolia</i>. Planta, 218(6):958-964
Fridovich L. 1977. The superoxide radical is an agene of oxygen toxicity superoxide dismutases provide an important defense. Bio sci, 27(7):462-464
Fu D Q, Zhu B Z, Zhu H L, Jiang W B, Luo Y B. 2005. Virus-induced gene silencing in tomato fruit. The Plant Journal, 43(2):299-308
Gautheret D, Poirot O, Lopez F, Audic S, Claverie J-M. 1998. Alternate Polyadenylation in Human mRNAs: A Large-Scale Analysis by EST clustering. Genome Research, 8(5):524-530 Gawienowski M C, Szymanski D, Perera I Y, Zielinski R E. 1993. Calmodulin Isoforms in Arabidopsis
Encoded by Multiple Divergent Messenger-Rnas[J]. Plant Mol Biol, 22 (2): 215-225 Gepstein S, Sabehi G, Carp M-J, Hajouj T, Nesher M F O, Yariv I, Dor C, Bassani M. 2003. Large-scale identification of leaf senescence-associated genes. The Plant Journal, 36(5):629-642
Gersdorff N, Muller M, Schall A, Miosge N. 2006. Secreted modular calcium-binding protein-1 localization during mouse embryogenesis. Histochemistry and Cell Biology, 126(6):705-712
Gilad A, Amitai-Zeigerson H, Bar-Zvi D, Scolnik P A. Asr1, a tomato water-stress regulated gene: genomic organization, developmental regulation and DNA-binding activity .1996. 447-454
Golodriga P Y,Troshin L P,Frolana L. 1986. Inhereritance of the character of seedlessness in the hybrid generation of V.vinifera. Tsitologyia Genetika, 19:372-376
Gordon D, Abajian C, Green P. 1998. Consed: A Graphical Tool for Sequence finishing. Genome Research, 8(3):195-202
Gordon D, Desmarais C, Green P. 2001. Automated finishing with antofinish.Genome Res, 11,614-625
Hanania U, Velcheva M, Or E, Flaishman M, Sahar N, Perl A. 2007. Silencing of chaperonin 21, that was differentially expressed in inflorescence of seedless and seeded grapes, promoted seed abortion in tobacco and tomato fruits. Transgenic Research, 16(4):515-525
Hanania U, Velcheva M, Sahar N, Flaishman M, Or E, Degani O, Perl A. 2009. The ubiquitin extension protein S27a is differentially expressed in developing flower organs of Thompson seedless versus Thompson seeded grape isogenic clones. Plant Cell Reports, 28(7):1033-1042
Harmon a C, Gribskov M, Harper J F. 2000. CDPKs - a kinase for every Ca2+ signal? Trends in Plant Science, 5(4):154-159
Hatey F, Tosser-Klopp G, Clouscard-Martinato C, Mulsant P, Gasser F. 1998. Expressed sequence tags for genes: a review. Genetics Selection Evolution, 30(6):1-21
Hong Y, Wang T-W, Hudak K A, Schade F, Froese C D, Thompson J E. 2000. An ethylene-induced cDNA encoding a lipase expressed at the onset of senescence. Proceedings of the National Academy of Sciences, 97(15):8717-8722
Huang J, Wang J.F, Zhang H S. 2004. Structure and function of plant C2H2 zinc finger protein. Hereditas (Beijing), 26: 414-418
Huang J, Wang J F, Wang Q H, Zhang H S. 2005. Identification of a rice zinc finger protein whose expression is transiently induced by drought, cold but not by salinity and abscisic acid. Mitochondrial DNA, 16(2):130-136
Huang J, Zhang H. 2007. The plant TFIIIA-type zinc finger proteins and their roles in abiotic stresstolerance. Yi Chuan, 29:915-922
Huang X, Madan A. 1999. CAP3: A DNA Sequence Assembly Program. Genome Research, 9(9):868-877
Ito T, Meyerowitz E M. 2000. Overexpression of a Gene Encoding a Cytochrome P450, CYP78A9, Induces Large and Seedless Fruit in Arabidopsis. The Plant Cell Online, 12(9):1541-1550
Iusem N D, Bartholomew D M, Hitz W D, Scolnik P A. 1993. Tomato (Lycopersicon esculentum) transcript induced by water deficit and ripening. Plant Physiology, 102(4):1353-1354
Jain D, Roy N, Chattopadhyay D, CaZF. 2009. A plant transcription factor functions through and parallel to HOG and calcineurin pathways in Saccharomyces cerevisiae to provide osmotolerance [J]. PLoS One ,4:e5154
Jeanneau M, Gerentes D, Foueillassar X, Zivy M, Vidal J, Toppan A, Perez P. 2002. Improvement of drought tolerance in maize: towards the functional validation of the Zm-Asr1 gene and increase of water use efficiency by over-expressing C4-PEPC. Biochimie, 84(11):1127-1135
Kalifa Y, Perlson E, Gilad A, Konrad Z, Scolnik P A, Bar-Zvi D. 2004. Over-expression of the water and salt stress-regulated Asr1 gene confers an increased salt tolerance. Plant, Cell & Environment, 27(12):1459-1468
Kalifa Y, Gilad A, Konrad Z, Zaccai M, Scolnik P A, Bar-Zvi D. 2004. The water-and salt-stress-regulated Asr1 (abscisic acid stress ripening) gene encodes a zinc-dependent DNA-binding protein. Biochemical Journal, 381(Pt 2):373-378
Kim J C, Lee S H, Cheong Y H, Yoo C M, Lee S I, Chun H J, Yun D J, Hong J C, Lee S Y, Lim C O. 2001. A novel cold‐inducible zinc finger protein from soybean, SCOF‐1, enhances cold tolerance in transgenic plants. The Plant Journal, 25(3):247-259
Kitashiba H, Iwai T, Toriyama K, Watanabe M, Hinata K. 1996. Identification of genes expressed in the shoot apex ofBrassica campestris during floral transition. Sexual plant reproduction, 9(3):186-188
Korkutal I, Celik S. 2005. Embryo abortion in some new seedless table grape (Vitis vinifera L.) varieties. International Journal of Botany, 1(1):1-4
Kotani H, Nakamura Y, Sato S, et al. 1998. Structural analysis of A rabidopsis thaliana chromosome 5.VI.Sequence features of the regions of 1,367,185bp covered by 19 physically assigned PI and TAC clones. DNA research, 5(3):203-216
Kudla J, Xu Q, Harter K, Gruissem W, Luan S. 1999. Genes for calcineurin B-like proteins in Arabidopsis are differentially regulated by stress signals. Proceedings of the National Academy of Sciences, 96 (8):4718-4723
Lahogue F, This P, Bouquet A. 1998. Identification of a codominant scar marker linked to the seedlessness character in grapevine. TAG Theoretical and Applied Genetics, 97(5):950-959
Lander E S, et al. 2001. International Human Genome Sequencing Consortium: Initial sequencing and analysis of the human genome. Nature, 409: 860–921
Ledbetter C A, Bougers L. 1994. Inheritance of stenspermocarpic seedless in Vitis vinifera L. The Journal of Heredity, 85(2):157-160
Ledbetter C A, Shonnard C B. 1991. Berry and seed characteristics associated with stenospermy in vinifera grapes. Journal of Horticultural Science, 66(2):247-252
Lers A, Jiang W B, Lomaniec E, Aharoni N. 1998. Proteins that functionally and immunogenically related to pathogenesis-related proteins are induced during parsley leaf senescence. Physiologia Plantarum,103:497-502
Li Q, Bettany A J, Donnison I, Griffiths C M, Thomas H, Scott I M. 2000. Characterisation of a cysteine protease cDNA from Lolium multiflorum leaves and its expression during senescence and cytokinin treatment. Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1492:233-236
Liu J P, Zhu J K. 1998. A calcium sensor homolog required for plant salt tolerance. Science, 280 (5371):1943-1945
Liu Y, Nakayama N, Schiff M, Litt A, Irish V F, Dinesh-Kumar S P. 2004. Virus induced gene silencing of a DEFICIENS ortholog in Nicotiana benthamiana. Plant Molecular Biology, 54 (5):701-711
Loomis N H, Weinberger J H. 1979. Inheritance studies of seedless in grape. Journal of American Sociey for Horticultural Science, 104(2):181-184
Lu J, Lamikanra O, Leong S. 1997. Induction of seedlessness in‘Triumph’muscadine grape(Vitis rotundifolia Michx.)by applying gibberellic acid. Hortscience, 32(1):89-90
Maskin L, Gubesblat G E, Moreno J E, Carrari F O, Frankel N, Sambade A, Rossi M, Iusem N D. 2001. Differential expression of the members of the Asr gene family in tomato (Lycopersicon esculentum) . Plant Science, 161:739-746
Mittler R, Kim Y, Song L, Coutu J, Coutu A, Ciftci-Yilmaz S, Lee H, Stevenson B, Zhu J K. 2006. Gain- and loss-of-function mutations in Zat10 enhance the tolerance of plants to abiotic stress. FEBS Letters, 580:6537-6542
Moser C, Segala C, Fontana P, Salakhudtinov I, Gatto P, Pindo M, Zyprian E, Toepfer R, Grando M S, Velasco R. 2005. Comparative analysis of expressed sequence tags from different organs of Vitis vinifera L. Functional & Interative Genomucs, 5:208-217
Nadeau J, Zhang X, Li J, O’Neill S. 1996. Ovule development: identification of stage specific and tissue-specific cDNAs. Plant Cell ,8:213-239
Notsuka K, Tsuru T, Shiraishi M. 2001. Seedless-seedless grape hybridization via in ovule embryo culture. Journal of the Japanese Society for Horticultural Science, 70(1):7-15
Pacey-Miller T, Scott K, Ablett E, Tingey S, Ching A, Henry R. 2003.Genes associated with the end of dormancy in grapes. Functional & Interative Genomucs, 3:144-152
Padmanabhan V, Dias D M A L, Newton R J. 1997. Expression analysis of a gene family in a loblolly pine (Pinus taeda L.) induced by water deficit stress. Plant Molecular Biology, 35: 801-807
Park S C, Sugimoto N, Larson M D, Beaudry R, Nocker S V. 2006. Identification of genes with potential roles in apple fruit development and biochemistry through large-scale statistical analysis of expressed sequence tags. Plant Physiology, 141:811-824
Pastori G M, Trippi V S. 1993. Antioxidative protection in a drought resistant strain during leaf senescence. Physiologia Plantarum, 87(2):227-231
Porter T D, Coon M J. 1991. Cytochrome P450. Multiplicity of iso2 forms, substrates, and calalytic and regulatory mechanisms. Journal of Biological Chemistry, 266 (21):13469-13472
Pratt L H, Liang C, Shah M, Sun F, Wang H, Reid S P, Gingle A R, Paterson A H, Wing R, Dean R, Klein R, Nguyen H T, Ma H M, Zhao X, Morishige D T, Mullet J E, Cordonnier-Pratt M M. 2005. Sorghum expressed. Sequence tags identify signature genes for drought, pathogenesis, and skotomorphogenesis from a milestone set of 16,801 unique transcripts. Plant Physiology, 139:869-884
Ruepp A, Maier D, Hani J, Tetko I, Mannhaupt G, Mewes HW. 2004. The FunCat, a functional annotationscheme for systematic classification of proteins from whole genomes. Nucleic Acids Research, 32: 5539–5545
Sakamoto H, Maruyama K, Sakuma Y, Meshi T, Iwabuchi M, Shinozaki K, Yamaguchi-Shinozaki K. 2004. Arabidopsis Cys2/His2-type zincfinger proteins function as transcription repressors under drought, cold, and high-salinity stress conditions. Plant Physiology, 136:2734-2746
Sato A H, Yamane M Y, Yoshinage K. 1994. Inheritance of seed-lessness in grape. Journal of the Japanese Society for Horticultural Science, 63(1):1-7
Schneider A, Salamini F, Gebhardt C. 1997. Expression patterns and promoter activity of the cold-regulated gene ci21A of potato. Plant Physiology, 113:335-345
Schuler G D, Boguski M S, Steuart E A, Stein L D, Gyapay G, Rice K, White R E, Rodriguez-ToméP, Aggarwal A,Bajorek E, Bentolila S, Birren B B, Butler A, Castle A B, Chiannilkulchai N, Chu A, Clee C, Cowles S,Day P J R, Dibling T, Drouot N, Dunham I, Duprat S, East C, Edwards C, Fan J B, Fang N, Fizames C, Garrett C, Green L, Hadley D, Harris M, Harrison P, Brady S, Hicks A, Holloway E, Hui L, Hussain S, Louis-Dit-Sully C, Ma J, MacGilvery A, Mader C, Maratukulam A, Matise T C, McKusick K B, Morissette J, Mungall A, Muselet D, Nusbaum H C, Page D C, Peck A, Perkins S, Piercy M, Qin F, Quackenbush J, Ranby S, Reif T, Rozen S, Sanders C, She X, Silva J, Slonim D K, Soderlund C, Sun W L, Tabar P, Thangarajah T, Vega-Czarny T, Vollrath D, Voyticky S, Wilmer T, Wu X, Adams M D, Auffray C, Walter N A R, Brandon R, Dehejia A, Goodfellow P N, Houlgatte R, Hudson Jr. J R, Ide S E, Iorio K R, Lee W Y, Seki N, Nagase T, Ishikawa K, Nomura N, Phillips C, Polymeropoulos M H, Sandusky M, Schmitt K, Berry R, Swanson K, Torres R, Venter J C, Sikela J M, Beckmann J S, Weissenbach J, Myers R M, Cox D R, James M R, Bentley D, Deloukas P, Lander E S, Hudson T J. 1996. A gene map of the human genome. Science, 274:540-546
Spiegel-Roy P, Baron J, Sahar N. 1990. Inheritance of seedlessness in seeded x seedless progeny of Vitis vinifera L. Vitis, 29:79-83
Stout A B. 1936. Seedlessness in grapes. In: New York State Agriculture Experimental Station. Tech Bull, Geneva, New york
Striem M J, Ben-Hayyim G, Spiegel-Roy. 1996. Identifying molecular genetic markers associated with seedlessness in grape. Journal of the American Society for Horticultural Sciences, 121(5):758-763
Striem M J, Ben-Hayyin G, Spiegel-Roy P. 1994. Developing molecular genetic markers for grape breeding using polymerase chain reaction procedures. Vitis, 33:53-54
Striem M J, Spiegel-Roy I B, Sahar N. 1992. The degree of development of the seed coat and the endosperm as separate subtraits of stenospermocarpic seedlessness in grapes. Vitis, 31: 149-155
Sugano S, Kaminaka H, Rybka Z, Catala R, Salinas J, Matsui K, Ohme-Takagi M, Takatsuji H. 2003.
Stress-responsive zinc finger gene ZPT2-3 plays a role in drought tolerance in petunia. The Plant Journal, 36:830-841
Tanabe S, Ashikari M, Fujioka S, Takatsuto S, Yoshida S, Yano M, Yoshimura A, Kitano H, Matsuoka M, Fujisawa Y, Kato H, Iwasaki Y. 2005. A novel cytochrome P450 is implicated in brassinosteroid biosynthesis via the characterization of a rice dwarf mutant, dwarf 11, with reduced seed length. Plant Cell, 17:776-790
Terrier N, Ageorges A, Abbal P, Romieu C. 2001. Generation of ESTs from grape berry at various developmental stages. Journal of Plant Physiology, 158:1575-1583
Jaillon O, Aury J-M, Noe, B, Policriti A, Clepet C, Casagrande A, Choisne N, Aubourg S, Vitulo N, Jubin C, Vezzi A, Legeai F, Hugueney P, Dasilva C, Horner D, Mica E, Jublot D Poulain, J, Bruyère C, Billault A, Segurens B, Gouyvenoux M, Ugarte E, Cattonaro F, Anthouard V, Vico V, Del Fabbro C, Alaux M, Di Gaspero G, Dumas V, Felice N, Paillard S, Juman I, Moroldo M, Scalabrin S, Canaguier A, Le Clainche I, Malacrida G, Durand E, Pesole G, Laucou V, Chatelet P, Merdinoglu D, Delledonne M, Pezzotti M, Lecharny A, Scarpelli, C, Artiguenave F, PèM E, Valle G, Morgante M, Caboche M, Adam-Blondon A-F, Weissenbach J, Quétier F, Wincker P. 2007. The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla, nature 27:463-469
Keilin T, Pang X Q, Venkateswari J, Halaly T, Crane O, Keren A, Ogrodovitch A, Ophir R, Volpin H, Galbraith D, Or E. 2007. Digital expression profiling of a grape-bud EST collection leads to new insight into molecular events during grape-bud dormancy release. Plant Science, 173:446-457
Hanania U, Velcheva M, Sahar N, Flaishman M, Or E, Degani O, Perl A. 2009. The ubiquitin extension protein S27a is differentially expressed in developing flower organs of Thompson seedless versus Thompson seeded grape isogenic clones . Plant Cell Reports, 28:1033-1042
Hanania U, Velcheva M, Sahar N, Flaishman M, Or E, Degani O, Perl A. 2007. Silencing of chaperonin 21, that was differentially expressed in inflorescence of seedless and seeded grapes, promoted seed abortion in tobacco and tomato fruits. Transgenic Research, 16(4):515-525
Venter J C, Adams M D, Myers E W, Li P W, Mural R J, Sutton G G, Smith H O, Yandell M, Evans C A, Holt R A, Gocayne J D, Amanatides P, Ballew R M, Huson D H, Wortman J R, Zhang Q, Kodira C D, Zheng X H, Chen L, Skupski M, Subramanian G, Thomas P D, Zhang J, Gabor Miklos G L, Nelson C, Broder S, Clark A G, Nadeau J, McKusick V A, Zinder N, Levine A J, Roberts R J, Simon M, Slayman C, Hunkapiller M, Bolanos R, Delcher A, Dew I, Fasulo D, Flanigan M, Florea L, Halpern A, Hannenhalli S, Kravitz S, Levy S, Mobarry C, Reinert K, Remington K, Abu-Threideh J, Beasley E, Biddick K, Bonazzi V, Brandon R, Cargill M, Chandramouliswaran I, Charlab R, Chaturvedi K, Deng Z, Francesco V D, Dunn P, Eilbeck K, Evangelista C, Gabrielian A E, Gan W, Ge W, Gong F, Gu Z, Guan P, Heiman T J, Higgins M E, Ji R-R, Ke Z, Ketchum K A, Lai Z, Lei Y, Li Z, Li J, Liang Y, Lin X, Lu F, Merkulov G V, Milshina N, Moore H M, Naik A K, Narayan V A, Neelam B, Nusskern D, Rusch D B, Salzberg S, Shao W, Shue B, Sun J, Wang Z Y, Wang A, Wang X, Wang J, Wei M-H, Wides R, Xiao C, Yan C, Yao A, Ye J, Zhan M, Zhang W, Zhang H, Zhao Q, Zheng L, Zhong F, Zhong W, Zhu SC, Zhao S, Gilbert D, Baumhueter S, Spier G, Carter C, Cravchik A, Woodage T, Ali F, An H, Awe A, Baldwin D, Baden H, Barnstead M, Barrow I, Beeson K, Busam D, Carver A, Center A, Cheng M L, Curry L, Danaher S, Davenport L, Desilets R, Dietz S, Dodson K, Doup L, Ferriera S, Garg N, Gluecksmann A, Hart B, Haynes J, Haynes C, Heiner C, Hladun S, Hostin D, Houck J, Howland T, Ibegwam C, Johnson J, Kalush F, Kline L, Koduru S, Love A, Mann F, May D, McCawley S, McIntosh T, McMullen I, Moy M, Moy L, Murphy B, Nelson K, Pfannkoch C, Pratts E, Puri V, Qureshi H, Reardon M, Rodriguez R, Rogers Y-H, Romblad D, Ruhfel B, Scott R, Sitter C, Smallwood M, Stewart E, Strong R, Suh E, Thomas R, Tint NN, Tse S, Vech C, Wang G, Wetter J, Williams S, Williams M, Windsor S, Winn-Deen E, Wolfe K, Zaveri J, Zaveri K, Abril JF, GuigóR, Campbell MJ, Sjolander KV, Karlak B, Kejariwal A, Mi H, Lazareva B, Hatton T, Narechania A, Diemer K, Muruganujan A, Guo N, Sato S, Bafna V, Istrail S, Lippert R, Schwartz R, Walenz B, Yooseph S, Allen D, Basu A, Baxendale J, Blick L, Caminha M, Carnes-Stine J, Caulk P, Chiang Y-H,Coyne M, Dahlke C, Mays A D, Dombroski M, Donnelly M, Ely D, Esparham S, Fosler C, Gire H, Glanowski S, Glasser K, Glodek A, Gorokhov M, Graham K, Gropman B, Harris M, Heil J, Henderson S, Hoover J, Jennings D, Jordan C, Jordan J, Kasha J, Kagan L, Kraft C, Levitsky A, Lewis
M, Liu X, Lopez J, Ma D, Majoros W, McDaniel J, Murphy S, Newman M, Nguyen T, Nguyen N, Nodell M, Pan S, Peck J, Peterson M, Rowe W, Sanders R, Scott J, Simpson M, Smith T, Sprague A, Stockwell T, Turner R, Venter E, Wang M, Wen M, Wu D, Wu M, Xia A, Zandieh A, Zhu X. 2001. The sequence of the human genome. Science, 291(5507):1304-51
Wang J W, Horiuchi S, Ogata T, Matui H. 1993. Relation between the formation of parthenocarpic berries and endogenous plant hormone contents in seedless grape cultivars. Journal of Japan Sociey for Horticultural Science, 62(1):9-14
Wang Y J, Lamikanra O, Lu J, Ramming, D W. 1996. Identification of genetic marker linked to seedless genes in grapes using RAPD. Acta Universitatis Agriculture Boreali-occidentalis, 24(5):1-10
Wang Y J. 1997. Lamikanra O. Analysis of sequencing the RAPD marker linked to seedless gene in grape. Acta Universitatis Agriculture Boreali-occidentalis, 25(4):1-5
Wang Y T, Yang C Y, Chen Y T, Lin Y, Shaw J Fu . 2004. Characterization of senescence-associated proteases in postharvest broccoli florets [J]. plaphy, 42(7-8):663-670
Webb S E, Miller A L. 2003. Calcium signalling during embryonic development. Nature Reviews Molecular Cell Biology, 4 (7):539-551
White C N, Rivin C J. 1995. Characterization and expression of a cDNA encoding a seed-specific metall othionein in maize. Plant Physiology, 108: 831-832
Xu D Q, Huang J, Guo S Q, Yang X, Bao Y M, Tang H J, Zhang H S. 2008. Overexpression of a TFIIIA-type zinc finger protein gene ZFP252 enhances drought and salt tolerance in rice (Oryza sativa L.). FEBS Letters, 582:1037-1043
Xu F X, Chye M L. 1999. Expression of cysteine proteinase during developmental events associated with programmed cell death in brinjal. The Plant Journal, 17:321-327
Yamane H. Ph D thesis. 1997. Studies on the breeding in grapes with reference to large berry and seedlessness [D]. Kyoto University, Japan
Yoon G M, Dowd P E, Gilroy S, McCubbin A G. 2006. Calcium-dependent protein kinase isoforms in Petunia have distinct functions in pollen tube growth, including regulating polarity. Plant Cell, 18 (4):867-878
Zhang J J, Wang Y J, Wang X P, Yang K Q, Yang J X. 2003. An Improved Method for Rapidly Extracting Total RNA from Vitis. Journal of Fruit Science, 20 (3):178-181
Zielinski R E. 2002. Characterization of three new members of the Arabidopsis thaliana calmodulin gene family: conserved and highly diverged members of the gene family functionally complement a yeast calmodulin null. Planta, 214 (3): 446-455
Zondlo S, Irish V. 1999. CYP78A5 encodes a cytochrome P450 that marks the shoot apical meristem boundary in Arabidopsis. The Plant Journal, 19:259-268
蒋迪军.1985.无核白等几个葡萄品种的细胞学研究.果树科学,21(2):10-21
孙亮先等.2002.水稻胚乳及幼苗基因表达谱的研究.[博士论文].杭州.浙江大学
王近卫,堀内昭作,林伯年等.1992.无核白葡萄的无核果形成的组织形态学研究.园艺学报,19(1):1-6
杨英军,王跃进,周鹏,等.2002.葡萄无核基因SCAR标记的序列构成与酶切分析.果树学报, 19(1):12-14
杨克强,王跃进,张今今,王西平,万怡震,张剑.2005.葡萄无核基因定位与作图的研究.遗传学报, 32(3):297-302
张宏明,孟新法,张潞生.1990.无核葡萄胚珠发育及早期离体培养的研究Ⅰ.激素对胚珠离体培养的影响.北京农业大学学报,16(3):277-282
张朝红,王跃进,王西平,杨亚州.2007.‘杨格尔’与‘京秀’葡萄胚珠发育过程中的活性氧代谢比较.园艺学报,34(6):1361-1366
张朝红,王跃进.2007.无核葡萄胚珠发育进程中EST的分析与败育相关基因的克隆.[博士论文].杨凌.西北农林科技大学
王飞,王跃进,周会玲,万怡震,杨进孝.2005.无核葡萄与中国野生葡萄杂种胚发育和败育的细胞学研究.西北农林科技大学(自然科学版),33(3):61-65
刘小宁,王跃进,张剑侠,江淑平.2005.Flame Seedless葡萄胚珠、胚乳及胚发育与败育的研究.西北植物学,25(10):1947-1953
Aaronson J S, Eckman B, Blevins R A, Borkowski J A, Myerson J, Imran S, Elliston K O. 1996. Toward the development of a gene index to the human genome: An assessment of the nature of high-throughput EST sequence data. Genome Research, 6(9):829-845
Ablett E, Seaton G, Scott K, Shelton D, Graham M W, Baverstock P, Lee L S, Henry R. 2000. Analysis of grape ESTs: global gene expression patterns in leaf and berry. Plant Science, 159(1):87-95
Adams M D, Dubnick M, Kerlavage a R, Moreno R, Kelley J M, Utterback T R, Nagle J W, Fields C, Venter J C. 1992. Sequence identification of 2,375 human brain genes. Nature, 355(6361):632-634
Adams M, Kelley J, Gocayne J, Dubnick M, Polymeropoulos M, Xiao H, Merril C, Wu A, Olde B, Moreno R, Et A. 1991. Complementary DNA sequencing: expressed sequence tags and human genome project. Science, 252(5013):1651-1656
Agarwal P, Arora R, Ray S, Singh A, Singh V, Takatsuji H, Kapoor S, Tyagi A. 2007. Genome-wide identification of C<sub>2</sub>H<sub>2</sub> zinc-finger gene family in rice and their phylogeny and expression analysis. Plant Molecular Biology, 65(4):467-485
Bishop J O, Morton J G, Rosbash M, Richardson M. 1974. Three abundance classes in HeLa cell messenger RNA. Nature, 250(463):199-204
Bouquet A, Danglot Y. 1996. Inheritance of seedlessness in grapevine (Vitis vinifera L). Vitis, 35(1):35-42
Cabezas J A, Cervera M T, Ruiz G, A L, Carre, O J, Mart, Nez-Zapater J M. 2006. A genetic analysis of seed and berry weight in grapevine. Genome, 49(12):1572-1585
Cakir B, Agasse A, Gaillard C, Saumonneau A, Delrot S, R A. 2003. A Grape ASR Protein Involved inSugar and Abscisic Acid Signaling. The Plant Cell Online, 15(9):2165-2180
Chatthai M, Kaukinen K H, Tranbarger T J, Gupta P K, Misra S. 1997. The isolation of a novel metallothionein-related cDNA expressed in somatic and zygotic embryos of Douglas-fir: regulation by ABA, osmoticum, and metal ions. Plant Molecular Biology, 34(2):243-254
Ciftci-Yilmaz S, Mittler R. 2008. The zinc finger network of plants. Cellular and Molecular Life Sciences, 65(7):1150-1160
Colova-Tsolova V, Jiang L, Perl A. 2003. Cyto-embryological aspects of seedlessness in Vitis vinifera L. and exploiting DNA recombinant technology as an advanced approach for introducing seedlessness into vinifera and muscadine grapes. Acta Hort, 603(1):195-199
da Silva, F.G, et al. 2005. Characterizing the grape transcriptome. Analysis of expressed sequence tags from multiple Vitis species and development of a compendium of gene expression during berry development. Plant Physiol, 139:574-597
Danielle Costenaro-da-Silvaa, GiselePassaiab, Jo?oA.P. Henriquesa, RogérioMargisa, Giancarlo Pasquali, LuísF.Revers. 2010. Identification and expression analysis of genes associated with the early berry development in the seedless grapevine (Vitis vinifera L.) cultivar Sultanine.Plant Science,179(5): 510-519
Degtyarenko K N, Archakov a I. 1993. Molecular evolution of P450 superfamily and P450-containing monooxygenase systems. FEBS Letters, 332(1-2):1-8
Delorme V G R, Mccabe P F, Kim D-J, Leaver C J. 2000. A Matrix Metalloproteinase Gene Is Expressed at the Boundary of Senescence and Programmed Cell Death in Cucumber. Plant Physiology, 123(3):917-928
Doligez a D, Bouquet a B, Danglot Y D, Lahogue F L, Riaz S R, Meredith C M, Edwards K E, This P T. 2002. Genetic mapping of grapevine (Vitis vinifera L.) applied to the detection of QTLs for seedless and berry weight. TAG Theoretical and Applied Genetics, 105(5):780-795
Stekel D J, Git Y, Falciani F. 2000. The Comparison of Gene Expression from Multiple cDNA Libraries. Genome Research, 10(12):2055-2061
Dure L, Crouch M, Harada J, Ho T-H D, Mundy J, Quatrano R, Thomas T, Sung Z R. 1989. Common amino acid sequence domains among the LEA proteins of higher plants. Plant Molecular Biology, 12(5):475-486
Ehlting J, Sauveplane V, Olry A, Ginglinger J F, Provart N, Werck-Reichhart D. 2008. An extensive (co-) expression analysis tool for the cytochrome P450 superfamily in Arabidopsis thaliana. BMC Plant Biology, 8(1):47
Ewing B, Green P. 1998. Base-Calling of Automated Sequencer Traces UsingPhred.II. Error probabilities. Genome Research, 8(3):186-194
Ewing B, Hillier L, Wendl M C, Green P. 1998. Base-calling of automated sequencer traces using phred. Genome Research, 8(3):175-185
Ewing R M, Kahla a B, Poirot O, Lopez F, Audic S, Claverie J-M. 1999. Large-Scale Statistical Analyses of Rice ESTs Reveal Correlated Patterns of Gene Expression. Genome Research, 9(10):950-959
Fred Y. Peng , Karen E. Reid , Nancy Liao , James Schlosser , Diego Lijavetzky , Robert Holt ,JoséM. Martínez Zapater , Steven Jones , Marco Marra , J?rg Bohlmann , Steven T. Lund. 2007. Generation of ESTs in Vitis vinifera wine grape (Cabernet Sauvignon) and table grape (Muscat Hamburg) anddiscovery of new candidate genes with potential roles in berry development. Gene, 402(1-2):40-50
Frey A, Godin B, Bonnet M, Sotta B, Marion-Poll A. 2004. Maternal synthesis of abscisic acid controls seed development and yield in <i>Nicotiana plumbaginifolia</i>. Planta, 218(6):958-964
Fridovich L. 1977. The superoxide radical is an agene of oxygen toxicity superoxide dismutases provide an important defense. Bio sci, 27(7):462-464
Fu D Q, Zhu B Z, Zhu H L, Jiang W B, Luo Y B. 2005. Virus-induced gene silencing in tomato fruit. The Plant Journal, 43(2):299-308
Gautheret D, Poirot O, Lopez F, Audic S, Claverie J-M. 1998. Alternate Polyadenylation in Human mRNAs: A Large-Scale Analysis by EST clustering. Genome Research, 8(5):524-530 Gawienowski M C, Szymanski D, Perera I Y, Zielinski R E. 1993. Calmodulin Isoforms in Arabidopsis
Encoded by Multiple Divergent Messenger-Rnas[J]. Plant Mol Biol, 22 (2): 215-225 Gepstein S, Sabehi G, Carp M-J, Hajouj T, Nesher M F O, Yariv I, Dor C, Bassani M. 2003. Large-scale identification of leaf senescence-associated genes. The Plant Journal, 36(5):629-642
Gersdorff N, Muller M, Schall A, Miosge N. 2006. Secreted modular calcium-binding protein-1 localization during mouse embryogenesis. Histochemistry and Cell Biology, 126(6):705-712
Gilad A, Amitai-Zeigerson H, Bar-Zvi D, Scolnik P A. Asr1, a tomato water-stress regulated gene: genomic organization, developmental regulation and DNA-binding activity .1996. 447-454
Golodriga P Y,Troshin L P,Frolana L. 1986. Inhereritance of the character of seedlessness in the hybrid generation of V.vinifera. Tsitologyia Genetika, 19:372-376
Gordon D, Abajian C, Green P. 1998. Consed: A Graphical Tool for Sequence finishing. Genome Research, 8(3):195-202
Gordon D, Desmarais C, Green P. 2001. Automated finishing with antofinish.Genome Res, 11,614-625
Hanania U, Velcheva M, Or E, Flaishman M, Sahar N, Perl A. 2007. Silencing of chaperonin 21, that was differentially expressed in inflorescence of seedless and seeded grapes, promoted seed abortion in tobacco and tomato fruits. Transgenic Research, 16(4):515-525
Hanania U, Velcheva M, Sahar N, Flaishman M, Or E, Degani O, Perl A. 2009. The ubiquitin extension protein S27a is differentially expressed in developing flower organs of Thompson seedless versus Thompson seeded grape isogenic clones. Plant Cell Reports, 28(7):1033-1042
Harmon a C, Gribskov M, Harper J F. 2000. CDPKs - a kinase for every Ca2+ signal? Trends in Plant Science, 5(4):154-159
Hatey F, Tosser-Klopp G, Clouscard-Martinato C, Mulsant P, Gasser F. 1998. Expressed sequence tags for genes: a review. Genetics Selection Evolution, 30(6):1-21
Hong Y, Wang T-W, Hudak K A, Schade F, Froese C D, Thompson J E. 2000. An ethylene-induced cDNA encoding a lipase expressed at the onset of senescence. Proceedings of the National Academy of Sciences, 97(15):8717-8722
Huang J, Wang J.F, Zhang H S. 2004. Structure and function of plant C2H2 zinc finger protein. Hereditas (Beijing), 26: 414-418
Huang J, Wang J F, Wang Q H, Zhang H S. 2005. Identification of a rice zinc finger protein whose expression is transiently induced by drought, cold but not by salinity and abscisic acid. Mitochondrial DNA, 16(2):130-136
Huang J, Zhang H. 2007. The plant TFIIIA-type zinc finger proteins and their roles in abiotic stresstolerance. Yi Chuan, 29:915-922
Huang X, Madan A. 1999. CAP3: A DNA Sequence Assembly Program. Genome Research, 9(9):868-877
Ito T, Meyerowitz E M. 2000. Overexpression of a Gene Encoding a Cytochrome P450, CYP78A9, Induces Large and Seedless Fruit in Arabidopsis. The Plant Cell Online, 12(9):1541-1550
Iusem N D, Bartholomew D M, Hitz W D, Scolnik P A. 1993. Tomato (Lycopersicon esculentum) transcript induced by water deficit and ripening. Plant Physiology, 102(4):1353-1354
Jain D, Roy N, Chattopadhyay D, CaZF. 2009. A plant transcription factor functions through and parallel to HOG and calcineurin pathways in Saccharomyces cerevisiae to provide osmotolerance [J]. PLoS One ,4:e5154
Jeanneau M, Gerentes D, Foueillassar X, Zivy M, Vidal J, Toppan A, Perez P. 2002. Improvement of drought tolerance in maize: towards the functional validation of the Zm-Asr1 gene and increase of water use efficiency by over-expressing C4-PEPC. Biochimie, 84(11):1127-1135
Kalifa Y, Perlson E, Gilad A, Konrad Z, Scolnik P A, Bar-Zvi D. 2004. Over-expression of the water and salt stress-regulated Asr1 gene confers an increased salt tolerance. Plant, Cell & Environment, 27(12):1459-1468
Kalifa Y, Gilad A, Konrad Z, Zaccai M, Scolnik P A, Bar-Zvi D. 2004. The water-and salt-stress-regulated Asr1 (abscisic acid stress ripening) gene encodes a zinc-dependent DNA-binding protein. Biochemical Journal, 381(Pt 2):373-378
Kim J C, Lee S H, Cheong Y H, Yoo C M, Lee S I, Chun H J, Yun D J, Hong J C, Lee S Y, Lim C O. 2001. A novel cold‐inducible zinc finger protein from soybean, SCOF‐1, enhances cold tolerance in transgenic plants. The Plant Journal, 25(3):247-259
Kitashiba H, Iwai T, Toriyama K, Watanabe M, Hinata K. 1996. Identification of genes expressed in the shoot apex ofBrassica campestris during floral transition. Sexual plant reproduction, 9(3):186-188
Korkutal I, Celik S. 2005. Embryo abortion in some new seedless table grape (Vitis vinifera L.) varieties. International Journal of Botany, 1(1):1-4
Kotani H, Nakamura Y, Sato S, et al. 1998. Structural analysis of A rabidopsis thaliana chromosome 5.VI.Sequence features of the regions of 1,367,185bp covered by 19 physically assigned PI and TAC clones. DNA research, 5(3):203-216
Kudla J, Xu Q, Harter K, Gruissem W, Luan S. 1999. Genes for calcineurin B-like proteins in Arabidopsis are differentially regulated by stress signals. Proceedings of the National Academy of Sciences, 96 (8):4718-4723
Lahogue F, This P, Bouquet A. 1998. Identification of a codominant scar marker linked to the seedlessness character in grapevine. TAG Theoretical and Applied Genetics, 97(5):950-959
Lander E S, et al. 2001. International Human Genome Sequencing Consortium: Initial sequencing and analysis of the human genome. Nature, 409: 860–921
Ledbetter C A, Bougers L. 1994. Inheritance of stenspermocarpic seedless in Vitis vinifera L. The Journal of Heredity, 85(2):157-160
Ledbetter C A, Shonnard C B. 1991. Berry and seed characteristics associated with stenospermy in vinifera grapes. Journal of Horticultural Science, 66(2):247-252
Lers A, Jiang W B, Lomaniec E, Aharoni N. 1998. Proteins that functionally and immunogenically related to pathogenesis-related proteins are induced during parsley leaf senescence. Physiologia Plantarum,103:497-502
Li Q, Bettany A J, Donnison I, Griffiths C M, Thomas H, Scott I M. 2000. Characterisation of a cysteine protease cDNA from Lolium multiflorum leaves and its expression during senescence and cytokinin treatment. Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1492:233-236
Liu J P, Zhu J K. 1998. A calcium sensor homolog required for plant salt tolerance. Science, 280 (5371):1943-1945
Liu Y, Nakayama N, Schiff M, Litt A, Irish V F, Dinesh-Kumar S P. 2004. Virus induced gene silencing of a DEFICIENS ortholog in Nicotiana benthamiana. Plant Molecular Biology, 54 (5):701-711
Loomis N H, Weinberger J H. 1979. Inheritance studies of seedless in grape. Journal of American Sociey for Horticultural Science, 104(2):181-184
Lu J, Lamikanra O, Leong S. 1997. Induction of seedlessness in‘Triumph’muscadine grape(Vitis rotundifolia Michx.)by applying gibberellic acid. Hortscience, 32(1):89-90
Maskin L, Gubesblat G E, Moreno J E, Carrari F O, Frankel N, Sambade A, Rossi M, Iusem N D. 2001. Differential expression of the members of the Asr gene family in tomato (Lycopersicon esculentum) . Plant Science, 161:739-746
Mittler R, Kim Y, Song L, Coutu J, Coutu A, Ciftci-Yilmaz S, Lee H, Stevenson B, Zhu J K. 2006. Gain- and loss-of-function mutations in Zat10 enhance the tolerance of plants to abiotic stress. FEBS Letters, 580:6537-6542
Moser C, Segala C, Fontana P, Salakhudtinov I, Gatto P, Pindo M, Zyprian E, Toepfer R, Grando M S, Velasco R. 2005. Comparative analysis of expressed sequence tags from different organs of Vitis vinifera L. Functional & Interative Genomucs, 5:208-217
Nadeau J, Zhang X, Li J, O’Neill S. 1996. Ovule development: identification of stage specific and tissue-specific cDNAs. Plant Cell ,8:213-239
Notsuka K, Tsuru T, Shiraishi M. 2001. Seedless-seedless grape hybridization via in ovule embryo culture. Journal of the Japanese Society for Horticultural Science, 70(1):7-15
Pacey-Miller T, Scott K, Ablett E, Tingey S, Ching A, Henry R. 2003.Genes associated with the end of dormancy in grapes. Functional & Interative Genomucs, 3:144-152
Padmanabhan V, Dias D M A L, Newton R J. 1997. Expression analysis of a gene family in a loblolly pine (Pinus taeda L.) induced by water deficit stress. Plant Molecular Biology, 35: 801-807
Park S C, Sugimoto N, Larson M D, Beaudry R, Nocker S V. 2006. Identification of genes with potential roles in apple fruit development and biochemistry through large-scale statistical analysis of expressed sequence tags. Plant Physiology, 141:811-824
Pastori G M, Trippi V S. 1993. Antioxidative protection in a drought resistant strain during leaf senescence. Physiologia Plantarum, 87(2):227-231
Porter T D, Coon M J. 1991. Cytochrome P450. Multiplicity of iso2 forms, substrates, and calalytic and regulatory mechanisms. Journal of Biological Chemistry, 266 (21):13469-13472
Pratt L H, Liang C, Shah M, Sun F, Wang H, Reid S P, Gingle A R, Paterson A H, Wing R, Dean R, Klein R, Nguyen H T, Ma H M, Zhao X, Morishige D T, Mullet J E, Cordonnier-Pratt M M. 2005. Sorghum expressed. Sequence tags identify signature genes for drought, pathogenesis, and skotomorphogenesis from a milestone set of 16,801 unique transcripts. Plant Physiology, 139:869-884
Ruepp A, Maier D, Hani J, Tetko I, Mannhaupt G, Mewes HW. 2004. The FunCat, a functional annotationscheme for systematic classification of proteins from whole genomes. Nucleic Acids Research, 32: 5539–5545
Sakamoto H, Maruyama K, Sakuma Y, Meshi T, Iwabuchi M, Shinozaki K, Yamaguchi-Shinozaki K. 2004. Arabidopsis Cys2/His2-type zincfinger proteins function as transcription repressors under drought, cold, and high-salinity stress conditions. Plant Physiology, 136:2734-2746
Sato A H, Yamane M Y, Yoshinage K. 1994. Inheritance of seed-lessness in grape. Journal of the Japanese Society for Horticultural Science, 63(1):1-7
Schneider A, Salamini F, Gebhardt C. 1997. Expression patterns and promoter activity of the cold-regulated gene ci21A of potato. Plant Physiology, 113:335-345
Schuler G D, Boguski M S, Steuart E A, Stein L D, Gyapay G, Rice K, White R E, Rodriguez-ToméP, Aggarwal A,Bajorek E, Bentolila S, Birren B B, Butler A, Castle A B, Chiannilkulchai N, Chu A, Clee C, Cowles S,Day P J R, Dibling T, Drouot N, Dunham I, Duprat S, East C, Edwards C, Fan J B, Fang N, Fizames C, Garrett C, Green L, Hadley D, Harris M, Harrison P, Brady S, Hicks A, Holloway E, Hui L, Hussain S, Louis-Dit-Sully C, Ma J, MacGilvery A, Mader C, Maratukulam A, Matise T C, McKusick K B, Morissette J, Mungall A, Muselet D, Nusbaum H C, Page D C, Peck A, Perkins S, Piercy M, Qin F, Quackenbush J, Ranby S, Reif T, Rozen S, Sanders C, She X, Silva J, Slonim D K, Soderlund C, Sun W L, Tabar P, Thangarajah T, Vega-Czarny T, Vollrath D, Voyticky S, Wilmer T, Wu X, Adams M D, Auffray C, Walter N A R, Brandon R, Dehejia A, Goodfellow P N, Houlgatte R, Hudson Jr. J R, Ide S E, Iorio K R, Lee W Y, Seki N, Nagase T, Ishikawa K, Nomura N, Phillips C, Polymeropoulos M H, Sandusky M, Schmitt K, Berry R, Swanson K, Torres R, Venter J C, Sikela J M, Beckmann J S, Weissenbach J, Myers R M, Cox D R, James M R, Bentley D, Deloukas P, Lander E S, Hudson T J. 1996. A gene map of the human genome. Science, 274:540-546
Spiegel-Roy P, Baron J, Sahar N. 1990. Inheritance of seedlessness in seeded x seedless progeny of Vitis vinifera L. Vitis, 29:79-83
Stout A B. 1936. Seedlessness in grapes. In: New York State Agriculture Experimental Station. Tech Bull, Geneva, New york
Striem M J, Ben-Hayyim G, Spiegel-Roy. 1996. Identifying molecular genetic markers associated with seedlessness in grape. Journal of the American Society for Horticultural Sciences, 121(5):758-763
Striem M J, Ben-Hayyin G, Spiegel-Roy P. 1994. Developing molecular genetic markers for grape breeding using polymerase chain reaction procedures. Vitis, 33:53-54
Striem M J, Spiegel-Roy I B, Sahar N. 1992. The degree of development of the seed coat and the endosperm as separate subtraits of stenospermocarpic seedlessness in grapes. Vitis, 31: 149-155
Sugano S, Kaminaka H, Rybka Z, Catala R, Salinas J, Matsui K, Ohme-Takagi M, Takatsuji H. 2003.
Stress-responsive zinc finger gene ZPT2-3 plays a role in drought tolerance in petunia. The Plant Journal, 36:830-841
Tanabe S, Ashikari M, Fujioka S, Takatsuto S, Yoshida S, Yano M, Yoshimura A, Kitano H, Matsuoka M, Fujisawa Y, Kato H, Iwasaki Y. 2005. A novel cytochrome P450 is implicated in brassinosteroid biosynthesis via the characterization of a rice dwarf mutant, dwarf 11, with reduced seed length. Plant Cell, 17:776-790
Terrier N, Ageorges A, Abbal P, Romieu C. 2001. Generation of ESTs from grape berry at various developmental stages. Journal of Plant Physiology, 158:1575-1583
Jaillon O, Aury J-M, Noe, B, Policriti A, Clepet C, Casagrande A, Choisne N, Aubourg S, Vitulo N, Jubin C, Vezzi A, Legeai F, Hugueney P, Dasilva C, Horner D, Mica E, Jublot D Poulain, J, Bruyère C, Billault A, Segurens B, Gouyvenoux M, Ugarte E, Cattonaro F, Anthouard V, Vico V, Del Fabbro C, Alaux M, Di Gaspero G, Dumas V, Felice N, Paillard S, Juman I, Moroldo M, Scalabrin S, Canaguier A, Le Clainche I, Malacrida G, Durand E, Pesole G, Laucou V, Chatelet P, Merdinoglu D, Delledonne M, Pezzotti M, Lecharny A, Scarpelli, C, Artiguenave F, PèM E, Valle G, Morgante M, Caboche M, Adam-Blondon A-F, Weissenbach J, Quétier F, Wincker P. 2007. The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla, nature 27:463-469
Keilin T, Pang X Q, Venkateswari J, Halaly T, Crane O, Keren A, Ogrodovitch A, Ophir R, Volpin H, Galbraith D, Or E. 2007. Digital expression profiling of a grape-bud EST collection leads to new insight into molecular events during grape-bud dormancy release. Plant Science, 173:446-457
Hanania U, Velcheva M, Sahar N, Flaishman M, Or E, Degani O, Perl A. 2009. The ubiquitin extension protein S27a is differentially expressed in developing flower organs of Thompson seedless versus Thompson seeded grape isogenic clones . Plant Cell Reports, 28:1033-1042
Hanania U, Velcheva M, Sahar N, Flaishman M, Or E, Degani O, Perl A. 2007. Silencing of chaperonin 21, that was differentially expressed in inflorescence of seedless and seeded grapes, promoted seed abortion in tobacco and tomato fruits. Transgenic Research, 16(4):515-525
Venter J C, Adams M D, Myers E W, Li P W, Mural R J, Sutton G G, Smith H O, Yandell M, Evans C A, Holt R A, Gocayne J D, Amanatides P, Ballew R M, Huson D H, Wortman J R, Zhang Q, Kodira C D, Zheng X H, Chen L, Skupski M, Subramanian G, Thomas P D, Zhang J, Gabor Miklos G L, Nelson C, Broder S, Clark A G, Nadeau J, McKusick V A, Zinder N, Levine A J, Roberts R J, Simon M, Slayman C, Hunkapiller M, Bolanos R, Delcher A, Dew I, Fasulo D, Flanigan M, Florea L, Halpern A, Hannenhalli S, Kravitz S, Levy S, Mobarry C, Reinert K, Remington K, Abu-Threideh J, Beasley E, Biddick K, Bonazzi V, Brandon R, Cargill M, Chandramouliswaran I, Charlab R, Chaturvedi K, Deng Z, Francesco V D, Dunn P, Eilbeck K, Evangelista C, Gabrielian A E, Gan W, Ge W, Gong F, Gu Z, Guan P, Heiman T J, Higgins M E, Ji R-R, Ke Z, Ketchum K A, Lai Z, Lei Y, Li Z, Li J, Liang Y, Lin X, Lu F, Merkulov G V, Milshina N, Moore H M, Naik A K, Narayan V A, Neelam B, Nusskern D, Rusch D B, Salzberg S, Shao W, Shue B, Sun J, Wang Z Y, Wang A, Wang X, Wang J, Wei M-H, Wides R, Xiao C, Yan C, Yao A, Ye J, Zhan M, Zhang W, Zhang H, Zhao Q, Zheng L, Zhong F, Zhong W, Zhu SC, Zhao S, Gilbert D, Baumhueter S, Spier G, Carter C, Cravchik A, Woodage T, Ali F, An H, Awe A, Baldwin D, Baden H, Barnstead M, Barrow I, Beeson K, Busam D, Carver A, Center A, Cheng M L, Curry L, Danaher S, Davenport L, Desilets R, Dietz S, Dodson K, Doup L, Ferriera S, Garg N, Gluecksmann A, Hart B, Haynes J, Haynes C, Heiner C, Hladun S, Hostin D, Houck J, Howland T, Ibegwam C, Johnson J, Kalush F, Kline L, Koduru S, Love A, Mann F, May D, McCawley S, McIntosh T, McMullen I, Moy M, Moy L, Murphy B, Nelson K, Pfannkoch C, Pratts E, Puri V, Qureshi H, Reardon M, Rodriguez R, Rogers Y-H, Romblad D, Ruhfel B, Scott R, Sitter C, Smallwood M, Stewart E, Strong R, Suh E, Thomas R, Tint NN, Tse S, Vech C, Wang G, Wetter J, Williams S, Williams M, Windsor S, Winn-Deen E, Wolfe K, Zaveri J, Zaveri K, Abril JF, GuigóR, Campbell MJ, Sjolander KV, Karlak B, Kejariwal A, Mi H, Lazareva B, Hatton T, Narechania A, Diemer K, Muruganujan A, Guo N, Sato S, Bafna V, Istrail S, Lippert R, Schwartz R, Walenz B, Yooseph S, Allen D, Basu A, Baxendale J, Blick L, Caminha M, Carnes-Stine J, Caulk P, Chiang Y-H,Coyne M, Dahlke C, Mays A D, Dombroski M, Donnelly M, Ely D, Esparham S, Fosler C, Gire H, Glanowski S, Glasser K, Glodek A, Gorokhov M, Graham K, Gropman B, Harris M, Heil J, Henderson S, Hoover J, Jennings D, Jordan C, Jordan J, Kasha J, Kagan L, Kraft C, Levitsky A, Lewis
M, Liu X, Lopez J, Ma D, Majoros W, McDaniel J, Murphy S, Newman M, Nguyen T, Nguyen N, Nodell M, Pan S, Peck J, Peterson M, Rowe W, Sanders R, Scott J, Simpson M, Smith T, Sprague A, Stockwell T, Turner R, Venter E, Wang M, Wen M, Wu D, Wu M, Xia A, Zandieh A, Zhu X. 2001. The sequence of the human genome. Science, 291(5507):1304-51
Wang J W, Horiuchi S, Ogata T, Matui H. 1993. Relation between the formation of parthenocarpic berries and endogenous plant hormone contents in seedless grape cultivars. Journal of Japan Sociey for Horticultural Science, 62(1):9-14
Wang Y J, Lamikanra O, Lu J, Ramming, D W. 1996. Identification of genetic marker linked to seedless genes in grapes using RAPD. Acta Universitatis Agriculture Boreali-occidentalis, 24(5):1-10
Wang Y J. 1997. Lamikanra O. Analysis of sequencing the RAPD marker linked to seedless gene in grape. Acta Universitatis Agriculture Boreali-occidentalis, 25(4):1-5
Wang Y T, Yang C Y, Chen Y T, Lin Y, Shaw J Fu . 2004. Characterization of senescence-associated proteases in postharvest broccoli florets [J]. plaphy, 42(7-8):663-670
Webb S E, Miller A L. 2003. Calcium signalling during embryonic development. Nature Reviews Molecular Cell Biology, 4 (7):539-551
White C N, Rivin C J. 1995. Characterization and expression of a cDNA encoding a seed-specific metall othionein in maize. Plant Physiology, 108: 831-832
Xu D Q, Huang J, Guo S Q, Yang X, Bao Y M, Tang H J, Zhang H S. 2008. Overexpression of a TFIIIA-type zinc finger protein gene ZFP252 enhances drought and salt tolerance in rice (Oryza sativa L.). FEBS Letters, 582:1037-1043
Xu F X, Chye M L. 1999. Expression of cysteine proteinase during developmental events associated with programmed cell death in brinjal. The Plant Journal, 17:321-327
Yamane H. Ph D thesis. 1997. Studies on the breeding in grapes with reference to large berry and seedlessness [D]. Kyoto University, Japan
Yoon G M, Dowd P E, Gilroy S, McCubbin A G. 2006. Calcium-dependent protein kinase isoforms in Petunia have distinct functions in pollen tube growth, including regulating polarity. Plant Cell, 18 (4):867-878
Zhang J J, Wang Y J, Wang X P, Yang K Q, Yang J X. 2003. An Improved Method for Rapidly Extracting Total RNA from Vitis. Journal of Fruit Science, 20 (3):178-181
Zielinski R E. 2002. Characterization of three new members of the Arabidopsis thaliana calmodulin gene family: conserved and highly diverged members of the gene family functionally complement a yeast calmodulin null. Planta, 214 (3): 446-455
Zondlo S, Irish V. 1999. CYP78A5 encodes a cytochrome P450 that marks the shoot apical meristem boundary in Arabidopsis. The Plant Journal, 19:259-268