“复方抗氧化制剂”及运动训练对大鼠肝组织自由基代谢和抗氧化能力的影响
|
摘要
选取64只雄性wistar大鼠,随机分为4组:即对照组、补剂组,训练组和训练补剂组。“复方抗氧化制剂”是以人参、枸杞、沙棘、核桃、芦荟等多种具有抗氧化作用的蔬菜、水果和药食同源的中草药为原料按一定的比例与纯净水配制而成。各组基础饲料饲养,适应性喂养1周后,训练组和训练补剂组在动物跑台上训练8周。9周后,随机将4组大鼠各分为两个亚组,既安静组和运动组处死。分别测定上述4组大鼠在安静状态时、定量负荷后不同功能状态下肝组织中丙二醛(MDA)、还原型谷胱甘肽(GSH)和蛋白含量,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)、黄嘌呤氧化酶(XOD)活性。所有数据采用SPSS12.0软件统计处理,组间比较采用t检验,显著性标准为P<0.05。统计数值均以平均数±标准差(X±SD)表示。实验结果表明:安静状态下,训练组以及训练补剂组MDA含量低于对照组,补剂组与对照组组间有显著性差异(P<0.05),训练补剂组低于训练组;补剂组、训练组以及训练补剂组GSH含量、GSH-PX、SOD活性皆高于对照组,其中训练补剂组GSH含量、补剂组SOD活性与对照组比较有显著性差异(P<0.05),补剂组CAT活性、训练组和训练补剂组SOD活性与对照组相比组间有显著性差异(P<0.01),其余3组XOD活性皆低于对照组,但无显著性差异(P>0.05)。定量负荷运动后,补剂组、训练组和训练补剂组MDA含量都低于对照组;训练组GSH含量与对照组比较有显著性差异(P<0.05),训练补剂组GSH含量与对照组组间有显著性差异(P<0.01);GSH-PX、CAT、SOD活性补剂组、训练组以及训练补剂组皆高于对照组,训练补剂组高于训练组,但没有显著性差异(P>0.05);4组XOD活性呈下降趋势。结论:“复方抗氧化制剂”能够降低大鼠肝组织中MDA含量,抑制脂质过氧化,能有效的清除自由基;“复方抗氧化制剂”能够提高大鼠肝组织中SOD、CAT、GSH-PX的活性和GSH的含量,提高机体在不同功能状态下的抗氧化能力;“复方抗氧化制剂”在安静状态下对于提高各种抗氧化酶的活性,抑止XOD的活性和降低MDA的含量较运动状态下效果更加明显。
64 male wistar mice were randomly divided into four groups: C (Control Group), T (Training Control Group), S (“Compound Antioxidants”Supplementation Group), TS (Training“Compound Antioxidants”Supplementation Group). Together with pure water by a certain ratio, the“compound antioxidants”beverage is made from sea buckthorn, ginseng, Chinese wolfberry, walnut and various vegetables, fruits, and Chinese herbal medicines that have antioxidant effect. Each group was fed with normal animal feed. After a week of feeding, the T and TS groups were both trained for 8 weeks on the animal run stage. After 9 weeks, the four groups were randomly divided into two sub-groups, i.e. the rest group and the trained group. The content of MDA, GSH and the activity of SOD, CAT, GSH-PX and XOD were measured respectively at the rest state and at the time of the fixed training load. All of the data have been processed by SPSS 12.0 software package with T-check for the comparison between groups and p<0.05 is taken as the standard for markedness. Results: at rest state, the content of MDA of the S, T and TS groups is lower than the C group. And the S group is much lower than the C group (p<0.05). The TS group is lower than the T group. The content of GSH and the activity of GSH-PX, CAT and SOD of the S, T and TS groups are all higher than the C group, among which the GSH content of the TS group is more remarkable than the C group (p<0.05), the activity of CAT of the S group is more remarkable than the C group (p<0.01), the activity of SOD of the S group is much higher than the C group (p<0.05), and the activity of SOD of the T and TS groups is more remarkable than the C group (p<0.01). Compared with the C group, the activity of XOD of the other three groups is lower than the C group, but with no significant difference (p>0.05). After fixed training load, the content of MDA of the S, T and TS groups is lower than the C group, the content of GSH of the T group is more remarkable than the C group (p<0.05 ), the content of GSH of the TS group is more remarkable than the C group (p<0.01), the activity of GSH-PX, CAT and SOD of the S, T and TS groups is more remarkable than the C group, among which that of the TS group is more remarkable than that of the T group, but with no significant difference (p>0.05). The activity of XOD of the four groups demonstrates a declining trend. Conclusions:“Compound antioxidants”and training can decrease the content of MDA of the liver tissue of the mice, inhibit rapid peroxidation and can effectively clear the free radical;“Compound antioxidants”and training can promote the activity of SOD, CAT, GSH-PX in the liver tissue and increase the content of GSH. It may help to improve the body’s antioxidant capability in different situations; At rest state,“compound antioxidants”can better promote the activity of various antioxidant enzymes, inhibit the activity of XOD and decrease the content of MDA than in the trained state; The changes of the different antioxidant enzymes in different situations are not the same. For some index, the difference is not significant. Further effort is needed to perfect the study.
64 male wistar mice were randomly divided into four groups: C (Control Group), T (Training Control Group), S (“Compound Antioxidants”Supplementation Group), TS (Training“Compound Antioxidants”Supplementation Group). Together with pure water by a certain ratio, the“compound antioxidants”beverage is made from sea buckthorn, ginseng, Chinese wolfberry, walnut and various vegetables, fruits, and Chinese herbal medicines that have antioxidant effect. Each group was fed with normal animal feed. After a week of feeding, the T and TS groups were both trained for 8 weeks on the animal run stage. After 9 weeks, the four groups were randomly divided into two sub-groups, i.e. the rest group and the trained group. The content of MDA, GSH and the activity of SOD, CAT, GSH-PX and XOD were measured respectively at the rest state and at the time of the fixed training load. All of the data have been processed by SPSS 12.0 software package with T-check for the comparison between groups and p<0.05 is taken as the standard for markedness. Results: at rest state, the content of MDA of the S, T and TS groups is lower than the C group. And the S group is much lower than the C group (p<0.05). The TS group is lower than the T group. The content of GSH and the activity of GSH-PX, CAT and SOD of the S, T and TS groups are all higher than the C group, among which the GSH content of the TS group is more remarkable than the C group (p<0.05), the activity of CAT of the S group is more remarkable than the C group (p<0.01), the activity of SOD of the S group is much higher than the C group (p<0.05), and the activity of SOD of the T and TS groups is more remarkable than the C group (p<0.01). Compared with the C group, the activity of XOD of the other three groups is lower than the C group, but with no significant difference (p>0.05). After fixed training load, the content of MDA of the S, T and TS groups is lower than the C group, the content of GSH of the T group is more remarkable than the C group (p<0.05 ), the content of GSH of the TS group is more remarkable than the C group (p<0.01), the activity of GSH-PX, CAT and SOD of the S, T and TS groups is more remarkable than the C group, among which that of the TS group is more remarkable than that of the T group, but with no significant difference (p>0.05). The activity of XOD of the four groups demonstrates a declining trend. Conclusions:“Compound antioxidants”and training can decrease the content of MDA of the liver tissue of the mice, inhibit rapid peroxidation and can effectively clear the free radical;“Compound antioxidants”and training can promote the activity of SOD, CAT, GSH-PX in the liver tissue and increase the content of GSH. It may help to improve the body’s antioxidant capability in different situations; At rest state,“compound antioxidants”can better promote the activity of various antioxidant enzymes, inhibit the activity of XOD and decrease the content of MDA than in the trained state; The changes of the different antioxidant enzymes in different situations are not the same. For some index, the difference is not significant. Further effort is needed to perfect the study.
引文
1.Cohen M V.Free radicals in ischem is and reper-fusion myocardical injury-Ann Intern Med 1989; 111:918
2.Cross CE,H olliwell B, Borish ET,et al. Oxygen radicals and human disease. Ann Intern Med 1987;107(4):526
3.周玫.自由基与疾病.第一军医大学学报,1982,2(3):303
4.SeijiM,et al. Sugery[J]. 1986; 99(2): 184
5.Bulkley GB, et al. Br. J Can[J]. 1982, 55(Supple):66
6.陈绍励.自由基及其在疾病与衰老中的作用[J].中国病理生理杂,1986,2(4):232-235
7.陈越,金久善.自由基的生物学效应[J].中国兽医杂志,1996,22(2):48-51
8.郭定宗,杨世锦,吴建光等.ND 野毒感染病鸡 MDA 含量和 SOD 活性研究[J].中国预防兽医学报,2000,22(5):365-367
9.HertzF.Pharmacologyoffreeradicals,recentviewson their relation to inflammatoryme chanisms[J].LifeSci,1984,34(8):713
10.McCord JM.Free Radic Biol Med,1988;4:9
11.Fridovich I.Ann Rev Pharmacol Toxicol,1983;23:239
12.Loviln R.Are indices of free radical damage related to exercises intensity European[ J].Journal of Applied Physiology,1987,56:313-316
13.金花,许豪文.耐力训练对大鼠大脑、小脑、心肌抗氧化酶及脂褐素含量的影响.中 国运动医学杂志,1995,14(3):166-169
14.史亚丽,任杰,许豪文.运动训练对小白鼠心肌、肝脏与股四头肌自由基代谢的影 响.山东体育学院学报,1994,10(3)16-20
15.王安利,池健,相子春.对有氧运动(游泳)抗衰老作用的研究(一)—游泳对不同月龄 小鼠自由基代谢的影响.北京体育大学学报,2000,23(4):474-477
16.刘洪珍,颜丽霞.有氧运动前、后肌组织自由基代谢和抗氧化酶变化特征的研究.曲 阜师范大学学报,1999,25(1):110
17.苗健,高琦,许恩来.微量元素与相关疾病[M] 河南:河南医科大学出版社,113
18.张复春,舒昌杰,韩从.硒对实验性肝损伤保护作用的研究[J].西安医科大学学报,1994,15(3):1114
19. 赵长峰.补充维生素 E 对大鼠运动能力及红细胞超氧化物歧化酶活性的影响.中国 运动医学杂志,1995,14(3):17
20. 朱蔚莉.维生素 E 对力竭小鼠股四头肌、脑组织自由基代谢的影响.体育科学 2002 ,22(5):108
21.田野主编.运动生理学高级教程.北京:高等教育出版社 2003,8
22.Palacios A, Piergiacom i VA, Catala A. V itam in A supplementation inhibits chem ilum inescence and lipid peroxidation in isolated rat liver m icrosomes and mtochondria[J].Mol Cell B ioch, 1996, 154: 77-81
23.刘友华.维生素 A 类化合物抗癌作用及其机制的研究进展[J].中国医科大学学报, 198 5, 14( 5) : 412-415
24.Stokke KT,et al.Changes in plasmaandredcellvolumes during exposre to higb altitude.Scand J Clin Lab Invest 1986;46(SuPPI.184):113
25.TodokoroK,etal.Specificb1ndingoferythropoietintoits recePtor on resPonsive rnouse erythroleukemia cells.Proc Natl Acad Sci USA 1987;84(12):412G
26.ZHANG H Y, CHEN D Z. Theoretical elucidation on activity differences of ten flavonoid antioxidants[J].Acta Biochimica Sinica, 2000, 32(4):317-321
27.Desai KN,WeiH,martiniere CA.The preveniiveandthera-Pe ntic Potential of the squalene.coniaining comPound,ROdex,on tumor Promotion and regression .Cancer Lett,1996,1 01(l):93-96
28.Malterud KE,FarbrotTL,Huse AE,et al .Antioxidant and adical scavenging effects of anihraquinones .Pharmacology,1993,47,Suppl,l:77-85
29.Sato Y,Kumazawa N,SuzukiM,et al .Studies on chemical protectors against radiation .XxXlll.protective mechanismsof various comPounds against skin injury induced by radiation. Yakugaku Zasshi,1 991,111(l):51-58
30.Hart LA,Nibbering pH,Barselaar Ml,et al .Effet of low molecular constituenis fro Aloc vera gel on oxidative metabolism and cytotoxic and bactericidal activities of human neutroPhils.Ini J lmmunoPharmacol,1990, 12(4):427-434
31.Hart LA,Enckevort PH,DikH,et al .Two functionally and che mically distinct immunomodulatory comPounds in the gel of Aloc vera .J EthnoPharmacol 1988,23(l):61-71
32.任岱,王一凡,姚银秀等.芦荟对超氧阴离子自由基链式反应的影响.中国现代医学杂志,1999,9(6):22-24
33.Davies KIA,Quintanilha Al,Brooks GA.Free radical and tissue damage Produced by exercise .Biochemical BioPhysical Research Communication,1982,107:1198 -1205
34.赵光,解黎明.蜂王浆对大鼠力竭运动能力影响的实验研究.四川体育科学,2003, 1:15-16
35.袁建华,冯炜权.磷脂对运动小鼠某些生化指标的改善作用.中国运动医学杂志, 2001,20(1):19
36.唐量,熊正英等.芦荟对运动训练小鼠肝组织自由基代谢及超微结构影响的实验研 究.中国运动医学杂志,2002 ,21(6):607
37.贺洪.人参皂甙 Rg1 对小鼠力竭游泳后恢复期骨骼肌自由基代谢的影响.中国运动医 学杂志,2002,21(6):610
38.朱梅菊,高顺生,熊静宇等.螺旋藻及其复方对小鼠运动能力和体内自由基代谢影响 的研究.体育学刊.2000,6:40-45
39.赖红梅.红芪多糖、力竭运动对大鼠自由基代谢的影响.西安体育学院学报,1998, 15(1):87-91
40.张钧,许豪文,郭勇力等.芦丁和维生素 C 对大鼠力竭运动后自由基代谢的影响.山东 体育学院学报,1997,13(3):28-31
41.中华医学杂志编辑委员会.脂质过氧化物与疾病专题讲座.中华医学杂志,1987; 6:641-651
42.陈可冀,孙燕,储大同等.黄芪临床应用研究.中西医结合杂志,1989: 9:327-330
43.王纲.自由基与中医中药.南京大学出版社,993;198,35-36
44.路雪雅等.没食子酸丙脂对乙醇诱导鼠肝急性损伤的保护作用.药学学报,1987; (8):586-590
45.魏文汉主编.病理生理学.1984;3(第 1 版)
46. 张勇等.耗竭性运动对大鼠心肌线粒体内膜流动性和复合体 I 活性的影响.生物化 学与生物物理学报.1995;27:338-340
47.张勇等.递增负荷力竭性运动对大鼠骨骼肌线粒体膜影响的 实验研究.生理通讯 (增刊).中国生理学会.1996.P68
48.曹兆丰等.运动性内源自由基对大鼠肝线粒体的影响.中国应用生理学杂志. 1993:9.25-28
49.Davies KJA, et al. Biochenical Biophysics Research Communica-tion, 1982; 107: 1198-1205
50.Till. Antioxidant enzyme response to exercise and aging[J]. M ed Sci Snort Exere. 1993, 25; 225: 31
51.刘丽萍等.游泳训练对大鼠肝组织自由基代谢及肝脏超微结构的影响 中国运动医学 杂志 1998.17.2 :121-123
52.丁莉等.银杏叶提取物对运动小鼠肝组织自由基代谢的影响中国运动医学杂志 1999 年第 18 卷第 4 期:312
53.许豪文.运动生物化学进展.1989
54.丁树哲.运动性内源自由基对大鼠心肌线粒体膜的影响.生物化学与生物物理学报 1991;23(4):305-310
55.李国莉等.枸杞多糖对运动训练小鼠耐力及体内自由基防御体系的影响 中国运动 医学杂志 1998 年第 17 卷第 1 期:56
56.黄力平等.Chin J Phys Med,June,1998,Vol 20 , No.2:84
57.韩丽莎,曾俐敏.沙棘对小鼠应激能力及一些血液生化指标的影响.内蒙医学杂 志,1999,31(2):72
58.孟浩,杭瑚.核桃仁活性成分的提取及体外抗氧化活性研究.食品科学,2001,22(12):44
59.唐量,熊正英.芦荟对运动训练小鼠脑组织自由基代谢的影响.陕西师范大学学 报,2003,30(2):99
60.Desai KN,WeiH,martiniere CA.The preveniiveandthera-Pe ntic Potential of the squalene.coniaining comPound,ROdex,on tumor Promotion and regression .Cancer Lett,1996,1 01(l):93-96
61.Malterud KE,FarbrotTL,Huse AE,et al .Antioxidant and adical scavenging effects of anihraquinones .Pharmacology,1993,47,Suppl,l:77-85
62.Sato Y,Kumazawa N,SuzukiM,et al .Studies on chemical protectors against radiation .XxXlll.protective mechanismsof various comPounds against skin injury induced by radiation. Yakugaku Zasshi,1 991,111(l):51-58
63.Hart LA,Nibbering pH,Barselaar Ml,et al .Effet of low molecular constituenis fro Aloc vera gel on oxidative metabolism and cytotoxic and bactericidal activities of human neutroPhils.Ini J lmmunoPharmacol,1990, 12(4):427-434
64.Hart LA,Enckevort PH,DikH,et al .Two functionally and che mically distinct immunomodulatory comPounds in the gel of Aloc vera .J EthnoPharmacol 1988, 3(l):61-71
65.Salminen A,et al.Endurance training reduce the susceptibility of mouse skeketal muscle to LPO in vitro.Acta.Physio.Scand.1983;117: 109-113
66.Suzuki M,et al.J.Nutr.Vitaminal,1983,29:141-151
67.Martine E,et al.Blochemistry and Biophysics,1989,887-890
68.刘洪珍.有氧运动对人体自由基代谢、抗氧化系统和其他血清酶活性的影响.北京体 育大学硕士学位论文,2001
69.Leeuwenburgh C,Hollander J,Leichtweis S.Adaptations of glutathione antioxidant system to endurance training are tissue and muscle fiber specific.Am J Physiol,1997,272:R363-R369
70.Los M,Schenck H,Hexel K,et al.IL-2 gene expression and NF-KB activation through CD28 requires reactive oxygen production by 5-lipoxygenase.EMBO J.1995,14:3731-3740
71.Quintanilha AT.Effects of physiol exercise and/or Vitamin E on tissue oxidative metabolism.Biochemical societyransactions.1984,12:403-404
72. SomaniS M,Rybak L P.Comparative effects of exercise training on transcription of antioxidant enzyme and the activity in old rat heart.Indian J Physiol Pharmaco,1996,40(3):205-212
73.刘洪珍,郭建军.不同有氧耐力训练对肌组织自由基代谢和抗氧化系统的影响.中国 运动医学杂志 2000,19(1):99
74.唐伟松,钟守先.氧自由基与缺血性损害.中华外科杂志,1991,29:260 262.
75.曹国华.运动、锌铜营养与自由基代谢 III.游泳训练对小鼠体内自由基生成与清除 的影响.中国运动医学杂志,1991,10(2):65
76.Kanter MM.et al.,Effect of exercise training on antionidant enzymes cardiotoxity of doxorubicin.J.Appl.Physiol.1985,59:1298-1303
77.郭世炳.运动对小鼠肌肉和肝脏中过氧化脂质及还原型谷胱甘肽含量的影响.中国运 动医学杂志, 1992 ,11(1):61
78.郭海英,许豪文.耐力训练对大鼠肝脏、股四头肌中谷光甘肽含量的影响.浙江体育 科学,1996,18(5):46
79.曹国华 .一次有氧运动或无氧运动对人体内自由基生成与消除的影响. 中国运动医 学杂志,1991 ,10(1):1
80.于基国,车保仁.不同强度的运动对肌组织自由基代谢及血清酶的影响.体育学 刊,1996,2:20-22
81.Caldarera CM,et al.Catalase and peroxidase activity in cardiac muscle. Bulletin of the Italian Experimental Biological Society ,1973,49:72-77
82.Ji LL,et al.Antioxidant enzyme systems in rat liver and skeletal muscle. Influence of selenium deficiency,chronictraining and acute exercise. Arch.Biochem.Biophys,1988,363:150-160
83.韩春华,王生,王元勋.胆红素对急性运动所致骨骼肌线粒体氧化应激的保护作用.中国运动医学杂志,2001,20(1):27-34
84.许豪文.1989 全国运动医学学术会议论文摘要汇编
85.Sen CK.Antioxidant and redox regulation of gene transcription.FASEB J. 1996,10:709-720
86.JjLL.Oxidative stress during exercise:implication of antioxidant nutrients.Free Radic Biol Med,1995,18(6):1079-1086
87.吴玲,陈吉棣.运动与生物膜结构和功能关系的研究进展.中国运动医学杂志,1995, 14(3):152-155
88. 张英等. 竹叶和银杏叶总黄酮含量及其抗自由基活性的比较研究.中国中药杂志, 2002,27(4):254
2.Cross CE,H olliwell B, Borish ET,et al. Oxygen radicals and human disease. Ann Intern Med 1987;107(4):526
3.周玫.自由基与疾病.第一军医大学学报,1982,2(3):303
4.SeijiM,et al. Sugery[J]. 1986; 99(2): 184
5.Bulkley GB, et al. Br. J Can[J]. 1982, 55(Supple):66
6.陈绍励.自由基及其在疾病与衰老中的作用[J].中国病理生理杂,1986,2(4):232-235
7.陈越,金久善.自由基的生物学效应[J].中国兽医杂志,1996,22(2):48-51
8.郭定宗,杨世锦,吴建光等.ND 野毒感染病鸡 MDA 含量和 SOD 活性研究[J].中国预防兽医学报,2000,22(5):365-367
9.HertzF.Pharmacologyoffreeradicals,recentviewson their relation to inflammatoryme chanisms[J].LifeSci,1984,34(8):713
10.McCord JM.Free Radic Biol Med,1988;4:9
11.Fridovich I.Ann Rev Pharmacol Toxicol,1983;23:239
12.Loviln R.Are indices of free radical damage related to exercises intensity European[ J].Journal of Applied Physiology,1987,56:313-316
13.金花,许豪文.耐力训练对大鼠大脑、小脑、心肌抗氧化酶及脂褐素含量的影响.中 国运动医学杂志,1995,14(3):166-169
14.史亚丽,任杰,许豪文.运动训练对小白鼠心肌、肝脏与股四头肌自由基代谢的影 响.山东体育学院学报,1994,10(3)16-20
15.王安利,池健,相子春.对有氧运动(游泳)抗衰老作用的研究(一)—游泳对不同月龄 小鼠自由基代谢的影响.北京体育大学学报,2000,23(4):474-477
16.刘洪珍,颜丽霞.有氧运动前、后肌组织自由基代谢和抗氧化酶变化特征的研究.曲 阜师范大学学报,1999,25(1):110
17.苗健,高琦,许恩来.微量元素与相关疾病[M] 河南:河南医科大学出版社,113
18.张复春,舒昌杰,韩从.硒对实验性肝损伤保护作用的研究[J].西安医科大学学报,1994,15(3):1114
19. 赵长峰.补充维生素 E 对大鼠运动能力及红细胞超氧化物歧化酶活性的影响.中国 运动医学杂志,1995,14(3):17
20. 朱蔚莉.维生素 E 对力竭小鼠股四头肌、脑组织自由基代谢的影响.体育科学 2002 ,22(5):108
21.田野主编.运动生理学高级教程.北京:高等教育出版社 2003,8
22.Palacios A, Piergiacom i VA, Catala A. V itam in A supplementation inhibits chem ilum inescence and lipid peroxidation in isolated rat liver m icrosomes and mtochondria[J].Mol Cell B ioch, 1996, 154: 77-81
23.刘友华.维生素 A 类化合物抗癌作用及其机制的研究进展[J].中国医科大学学报, 198 5, 14( 5) : 412-415
24.Stokke KT,et al.Changes in plasmaandredcellvolumes during exposre to higb altitude.Scand J Clin Lab Invest 1986;46(SuPPI.184):113
25.TodokoroK,etal.Specificb1ndingoferythropoietintoits recePtor on resPonsive rnouse erythroleukemia cells.Proc Natl Acad Sci USA 1987;84(12):412G
26.ZHANG H Y, CHEN D Z. Theoretical elucidation on activity differences of ten flavonoid antioxidants[J].Acta Biochimica Sinica, 2000, 32(4):317-321
27.Desai KN,WeiH,martiniere CA.The preveniiveandthera-Pe ntic Potential of the squalene.coniaining comPound,ROdex,on tumor Promotion and regression .Cancer Lett,1996,1 01(l):93-96
28.Malterud KE,FarbrotTL,Huse AE,et al .Antioxidant and adical scavenging effects of anihraquinones .Pharmacology,1993,47,Suppl,l:77-85
29.Sato Y,Kumazawa N,SuzukiM,et al .Studies on chemical protectors against radiation .XxXlll.protective mechanismsof various comPounds against skin injury induced by radiation. Yakugaku Zasshi,1 991,111(l):51-58
30.Hart LA,Nibbering pH,Barselaar Ml,et al .Effet of low molecular constituenis fro Aloc vera gel on oxidative metabolism and cytotoxic and bactericidal activities of human neutroPhils.Ini J lmmunoPharmacol,1990, 12(4):427-434
31.Hart LA,Enckevort PH,DikH,et al .Two functionally and che mically distinct immunomodulatory comPounds in the gel of Aloc vera .J EthnoPharmacol 1988,23(l):61-71
32.任岱,王一凡,姚银秀等.芦荟对超氧阴离子自由基链式反应的影响.中国现代医学杂志,1999,9(6):22-24
33.Davies KIA,Quintanilha Al,Brooks GA.Free radical and tissue damage Produced by exercise .Biochemical BioPhysical Research Communication,1982,107:1198 -1205
34.赵光,解黎明.蜂王浆对大鼠力竭运动能力影响的实验研究.四川体育科学,2003, 1:15-16
35.袁建华,冯炜权.磷脂对运动小鼠某些生化指标的改善作用.中国运动医学杂志, 2001,20(1):19
36.唐量,熊正英等.芦荟对运动训练小鼠肝组织自由基代谢及超微结构影响的实验研 究.中国运动医学杂志,2002 ,21(6):607
37.贺洪.人参皂甙 Rg1 对小鼠力竭游泳后恢复期骨骼肌自由基代谢的影响.中国运动医 学杂志,2002,21(6):610
38.朱梅菊,高顺生,熊静宇等.螺旋藻及其复方对小鼠运动能力和体内自由基代谢影响 的研究.体育学刊.2000,6:40-45
39.赖红梅.红芪多糖、力竭运动对大鼠自由基代谢的影响.西安体育学院学报,1998, 15(1):87-91
40.张钧,许豪文,郭勇力等.芦丁和维生素 C 对大鼠力竭运动后自由基代谢的影响.山东 体育学院学报,1997,13(3):28-31
41.中华医学杂志编辑委员会.脂质过氧化物与疾病专题讲座.中华医学杂志,1987; 6:641-651
42.陈可冀,孙燕,储大同等.黄芪临床应用研究.中西医结合杂志,1989: 9:327-330
43.王纲.自由基与中医中药.南京大学出版社,993;198,35-36
44.路雪雅等.没食子酸丙脂对乙醇诱导鼠肝急性损伤的保护作用.药学学报,1987; (8):586-590
45.魏文汉主编.病理生理学.1984;3(第 1 版)
46. 张勇等.耗竭性运动对大鼠心肌线粒体内膜流动性和复合体 I 活性的影响.生物化 学与生物物理学报.1995;27:338-340
47.张勇等.递增负荷力竭性运动对大鼠骨骼肌线粒体膜影响的 实验研究.生理通讯 (增刊).中国生理学会.1996.P68
48.曹兆丰等.运动性内源自由基对大鼠肝线粒体的影响.中国应用生理学杂志. 1993:9.25-28
49.Davies KJA, et al. Biochenical Biophysics Research Communica-tion, 1982; 107: 1198-1205
50.Till. Antioxidant enzyme response to exercise and aging[J]. M ed Sci Snort Exere. 1993, 25; 225: 31
51.刘丽萍等.游泳训练对大鼠肝组织自由基代谢及肝脏超微结构的影响 中国运动医学 杂志 1998.17.2 :121-123
52.丁莉等.银杏叶提取物对运动小鼠肝组织自由基代谢的影响中国运动医学杂志 1999 年第 18 卷第 4 期:312
53.许豪文.运动生物化学进展.1989
54.丁树哲.运动性内源自由基对大鼠心肌线粒体膜的影响.生物化学与生物物理学报 1991;23(4):305-310
55.李国莉等.枸杞多糖对运动训练小鼠耐力及体内自由基防御体系的影响 中国运动 医学杂志 1998 年第 17 卷第 1 期:56
56.黄力平等.Chin J Phys Med,June,1998,Vol 20 , No.2:84
57.韩丽莎,曾俐敏.沙棘对小鼠应激能力及一些血液生化指标的影响.内蒙医学杂 志,1999,31(2):72
58.孟浩,杭瑚.核桃仁活性成分的提取及体外抗氧化活性研究.食品科学,2001,22(12):44
59.唐量,熊正英.芦荟对运动训练小鼠脑组织自由基代谢的影响.陕西师范大学学 报,2003,30(2):99
60.Desai KN,WeiH,martiniere CA.The preveniiveandthera-Pe ntic Potential of the squalene.coniaining comPound,ROdex,on tumor Promotion and regression .Cancer Lett,1996,1 01(l):93-96
61.Malterud KE,FarbrotTL,Huse AE,et al .Antioxidant and adical scavenging effects of anihraquinones .Pharmacology,1993,47,Suppl,l:77-85
62.Sato Y,Kumazawa N,SuzukiM,et al .Studies on chemical protectors against radiation .XxXlll.protective mechanismsof various comPounds against skin injury induced by radiation. Yakugaku Zasshi,1 991,111(l):51-58
63.Hart LA,Nibbering pH,Barselaar Ml,et al .Effet of low molecular constituenis fro Aloc vera gel on oxidative metabolism and cytotoxic and bactericidal activities of human neutroPhils.Ini J lmmunoPharmacol,1990, 12(4):427-434
64.Hart LA,Enckevort PH,DikH,et al .Two functionally and che mically distinct immunomodulatory comPounds in the gel of Aloc vera .J EthnoPharmacol 1988, 3(l):61-71
65.Salminen A,et al.Endurance training reduce the susceptibility of mouse skeketal muscle to LPO in vitro.Acta.Physio.Scand.1983;117: 109-113
66.Suzuki M,et al.J.Nutr.Vitaminal,1983,29:141-151
67.Martine E,et al.Blochemistry and Biophysics,1989,887-890
68.刘洪珍.有氧运动对人体自由基代谢、抗氧化系统和其他血清酶活性的影响.北京体 育大学硕士学位论文,2001
69.Leeuwenburgh C,Hollander J,Leichtweis S.Adaptations of glutathione antioxidant system to endurance training are tissue and muscle fiber specific.Am J Physiol,1997,272:R363-R369
70.Los M,Schenck H,Hexel K,et al.IL-2 gene expression and NF-KB activation through CD28 requires reactive oxygen production by 5-lipoxygenase.EMBO J.1995,14:3731-3740
71.Quintanilha AT.Effects of physiol exercise and/or Vitamin E on tissue oxidative metabolism.Biochemical societyransactions.1984,12:403-404
72. SomaniS M,Rybak L P.Comparative effects of exercise training on transcription of antioxidant enzyme and the activity in old rat heart.Indian J Physiol Pharmaco,1996,40(3):205-212
73.刘洪珍,郭建军.不同有氧耐力训练对肌组织自由基代谢和抗氧化系统的影响.中国 运动医学杂志 2000,19(1):99
74.唐伟松,钟守先.氧自由基与缺血性损害.中华外科杂志,1991,29:260 262.
75.曹国华.运动、锌铜营养与自由基代谢 III.游泳训练对小鼠体内自由基生成与清除 的影响.中国运动医学杂志,1991,10(2):65
76.Kanter MM.et al.,Effect of exercise training on antionidant enzymes cardiotoxity of doxorubicin.J.Appl.Physiol.1985,59:1298-1303
77.郭世炳.运动对小鼠肌肉和肝脏中过氧化脂质及还原型谷胱甘肽含量的影响.中国运 动医学杂志, 1992 ,11(1):61
78.郭海英,许豪文.耐力训练对大鼠肝脏、股四头肌中谷光甘肽含量的影响.浙江体育 科学,1996,18(5):46
79.曹国华 .一次有氧运动或无氧运动对人体内自由基生成与消除的影响. 中国运动医 学杂志,1991 ,10(1):1
80.于基国,车保仁.不同强度的运动对肌组织自由基代谢及血清酶的影响.体育学 刊,1996,2:20-22
81.Caldarera CM,et al.Catalase and peroxidase activity in cardiac muscle. Bulletin of the Italian Experimental Biological Society ,1973,49:72-77
82.Ji LL,et al.Antioxidant enzyme systems in rat liver and skeletal muscle. Influence of selenium deficiency,chronictraining and acute exercise. Arch.Biochem.Biophys,1988,363:150-160
83.韩春华,王生,王元勋.胆红素对急性运动所致骨骼肌线粒体氧化应激的保护作用.中国运动医学杂志,2001,20(1):27-34
84.许豪文.1989 全国运动医学学术会议论文摘要汇编
85.Sen CK.Antioxidant and redox regulation of gene transcription.FASEB J. 1996,10:709-720
86.JjLL.Oxidative stress during exercise:implication of antioxidant nutrients.Free Radic Biol Med,1995,18(6):1079-1086
87.吴玲,陈吉棣.运动与生物膜结构和功能关系的研究进展.中国运动医学杂志,1995, 14(3):152-155
88. 张英等. 竹叶和银杏叶总黄酮含量及其抗自由基活性的比较研究.中国中药杂志, 2002,27(4):254