内蒙古地区野生罗布麻遗传多样性研究
|
摘要
罗布麻(Apocynum venetum Linn.)为多年生植物,具有耐盐碱、干旱、酷暑、寒冷、贫瘠等优良抗性。罗布麻用途广泛,可药用、也是良好的饲用、纤维、绿化和蜜源植物。目前,我国北方地区罗布麻由于生态环境恶化及人为过度利用,资源量锐减,如何保护和合理利用罗布麻种质资源已成为一项重要的研究课题。本试验对内蒙古地区野生罗布麻的形态性状、AFLP、ISSR及染色体数目和POD同工酶表型进行了遗传多样性分析,旨在明确该地区罗布麻种质资源的多样性状况及亲缘关系,为其合理开发利用提供依据。主要结果如下:
1.内蒙古通辽、包头、巴彦淖尔3个地区罗布麻的株高、茎粗、节间长、分枝长、花序长、果荚长、种子长、叶片、长宽等16个表型性状的总多样性指数为1.858,居群内多样性指数为0.862,居群间的多样性指数为0.996,居群内遗传多样性为46.39%,居群间遗传多样性为53.61%。罗布麻不同形态性状的多样性指数差异较大,其中株高、茎粗、节数、节间长、分枝长、千粒重、种子宽、果荚长、顶花序长、花萼长、托叶长、叶长、叶宽的多样性指数较高,容易受到环境的影响,是引起形态变异的主要性状。
2.内蒙古3个地区罗布麻形态性状聚类结果:通辽地区单独列为1类,包头地区和巴彦淖尔地区为另1类,包头地区与巴彦淖尔地区的罗布麻亲缘关系较近。
3.内蒙古3个地区45份罗布麻材料的DNA扩增得到5359个AFLP位点,多态性位点比例为91.22%。罗布麻AFLP遗传多样性指数为1.286,居群内为0.533,居群间为0.753,居群内的分化系数为41.45%,居群间的分化系数为58.55%,居群内和居群间的变异均为罗布麻种质变异的主要来源。3个不同地区罗布麻的相异系数变幅为0.0042～0.819,以相异系数值0.13为基准,通辽地区的绝大部分材料聚为1类,包头和巴彦淖尔地区的绝大部分材料聚为1类,通辽地区罗布麻种质资源与包头和巴彦淖尔地区的亲缘关系较远。
4.利用6个ISSR适宜引物扩增出65个ISSR位点,多态性位点55个,多态性位点比例为84.62%。内蒙古地区罗布麻ISSR遗传多样性指数为0.424,居群内为0.197,居群间为0.227,居群间的遗传分化系数为53.54%。以相异系数0.44为基准分为2类:通辽地区的种质材料聚为1类,地理位置相距较近的巴彦淖尔地区和包头地区的种质材料聚为另1类,包头和巴彦淖尔地区罗布麻种质资源的亲缘关系较近。
5.内蒙古通辽地区的染色体数目为20条(2n=2x=20),包头和巴彦淖尔地区的染色体数目均为16条(2n=2x=16),内蒙古东西部地区罗布麻染色体数目存在差异。
6.内蒙古通辽、巴彦淖尔、包头3个地区罗布麻苗期叶、茎、根不同器官的POD同工酶酶谱表型存在一定差异,遗传多样性较丰富。
As an perennial plant, Apocynum venetum Linn. is well known for its high resistance to salinity, drought, heat and leanness as well as being wildly used as medicine, fiber, ornamental plant, forage and nectariferous plant. Currently, Apocynum venetum Linn. resources in north China have been decreasing sharply owing to the environmental deterioration and abuse of human being, therefore, how to preserve and reasonably utilize Apocynum venetum Linn. resources turns into an important research topic. In this dissertation, the genetic diversity of wild Apocynum venetum Linn. in Inner Mongolia was analyzed based on morphological characteristics, AFLP, ISSR, chromosome number and POD isoenzyme phenotype to confirm the diversity of Apocynum venetum Linn. germplasm and their genetic relationship in this region, and thereby to set up the background for reasonable exploitation and utilization of Apocynum venetum Linn.. The results showed that:
1. The total diversity index, diversity index within population, diversity index among populations, genetic diversity within and among populations were 1.858, 0.862, 0.996, 46.39% and 53.61%, respectively, which derived from 16 phenotypic values including plant height, stem thickness, internode length, branch length, inflorescence length, pod length, seed length, leaf length and leaf width of Apocynum venetum Linn.populations originated from Tongliao, Baotou and Bayannaoer. There were big differences among the diversity index of morphological parameters in Apocynum venetum Linn.. The higher diversity index was observed in plant height, stem thickness, node number, internode length, branch length, thousand-seed weight, seed width, pod length, top inflorescence length, calyx length, stipule length, leaf length and leaf width, indicating that these were the major characteristics giving rise to the morphological variance and were readily affected by the environment.
2. The clustering of populations of Apocynum venetum Linn. originating from 3 regions of Inner Mongolia based on morphological properties showed that populations of Apocynum venetum Linn. from Tongliao was classified into one group while Apocynum venetum Linn. from Baotou and Bayannaoer belonged to another group, indicating that there was close relationship between Apocynum venetum Linn. populations from Baotou and Bayannaoer.
3. 5359 AFLP loci were found by DNA amplification with 45 Apocynum venetum Linn. populations from 3 regions, with 91.22% polymorphic loci. AFLP genetic diversity index, AFLP genetic diversity index within population, AFLP genetic diversity index among populations, differentiation coefficient within population and differentiation coefficient among populations were 1.286, 0.533, 0.753, 41.45% and 58.55%, respectively. The variance of Apocynum venetum Linn. germplasm was mainly derived from variance within population and among populations. Coefficient of difference in Apocynum venetum Linn. from 3 regions varied between 0.0042 and 0.819. Regarding coefficient of difference as a benchmark, most of Apocynum venetum Linn. populations from Tongliao region were sorted into one group while most of Apocynum venetum Linn. populations from Baotou and Bayannaoer were categorized to another group, showing that the relationship between Tongliao group and Baotou group was wide.
4. 65 ISSR loci, 55 polymorphic loci were observed by amplification using 6 ISSR primers. 84.62% were polymorphic loci. ISSR genetic diversity index, ISSR genetic diversity index within population, ISSR genetic diversity index among population and genetic differentiation coefficient were 0.424, 0.197, 0.227 and 53.54%, respectively. Regarding coefficient of difference as a benchmark, Apocynum venetum Linn. populations from Tongliao was classified into one group, while Apocynum venetum Linn. populations from Baotou and Bayannaoer came to another group, indicating there was close relationship between Apocynum venetum Linn. populations from Baotou and Bayannaoer.
5. 20 (2n =2x =20) chromosomes were observed in Apocynum venetum Linn. from Tongliao while 16(2n =2x =16) chromosomes in Apocynum venetum Linn. from Baotou and Bayannaoer. This result demonstrated that there was difference in chromosome number among Apocynum venetum Linn. populations from west to east of Inner Mongolia.
6. POD isoenzyme phenotypic zymograms at different organs (leaf, stem and root) showed some differences among Apocynum venetum Linn. populations originated from 3 different regions.
1.内蒙古通辽、包头、巴彦淖尔3个地区罗布麻的株高、茎粗、节间长、分枝长、花序长、果荚长、种子长、叶片、长宽等16个表型性状的总多样性指数为1.858,居群内多样性指数为0.862,居群间的多样性指数为0.996,居群内遗传多样性为46.39%,居群间遗传多样性为53.61%。罗布麻不同形态性状的多样性指数差异较大,其中株高、茎粗、节数、节间长、分枝长、千粒重、种子宽、果荚长、顶花序长、花萼长、托叶长、叶长、叶宽的多样性指数较高,容易受到环境的影响,是引起形态变异的主要性状。
2.内蒙古3个地区罗布麻形态性状聚类结果:通辽地区单独列为1类,包头地区和巴彦淖尔地区为另1类,包头地区与巴彦淖尔地区的罗布麻亲缘关系较近。
3.内蒙古3个地区45份罗布麻材料的DNA扩增得到5359个AFLP位点,多态性位点比例为91.22%。罗布麻AFLP遗传多样性指数为1.286,居群内为0.533,居群间为0.753,居群内的分化系数为41.45%,居群间的分化系数为58.55%,居群内和居群间的变异均为罗布麻种质变异的主要来源。3个不同地区罗布麻的相异系数变幅为0.0042～0.819,以相异系数值0.13为基准,通辽地区的绝大部分材料聚为1类,包头和巴彦淖尔地区的绝大部分材料聚为1类,通辽地区罗布麻种质资源与包头和巴彦淖尔地区的亲缘关系较远。
4.利用6个ISSR适宜引物扩增出65个ISSR位点,多态性位点55个,多态性位点比例为84.62%。内蒙古地区罗布麻ISSR遗传多样性指数为0.424,居群内为0.197,居群间为0.227,居群间的遗传分化系数为53.54%。以相异系数0.44为基准分为2类:通辽地区的种质材料聚为1类,地理位置相距较近的巴彦淖尔地区和包头地区的种质材料聚为另1类,包头和巴彦淖尔地区罗布麻种质资源的亲缘关系较近。
5.内蒙古通辽地区的染色体数目为20条(2n=2x=20),包头和巴彦淖尔地区的染色体数目均为16条(2n=2x=16),内蒙古东西部地区罗布麻染色体数目存在差异。
6.内蒙古通辽、巴彦淖尔、包头3个地区罗布麻苗期叶、茎、根不同器官的POD同工酶酶谱表型存在一定差异,遗传多样性较丰富。
As an perennial plant, Apocynum venetum Linn. is well known for its high resistance to salinity, drought, heat and leanness as well as being wildly used as medicine, fiber, ornamental plant, forage and nectariferous plant. Currently, Apocynum venetum Linn. resources in north China have been decreasing sharply owing to the environmental deterioration and abuse of human being, therefore, how to preserve and reasonably utilize Apocynum venetum Linn. resources turns into an important research topic. In this dissertation, the genetic diversity of wild Apocynum venetum Linn. in Inner Mongolia was analyzed based on morphological characteristics, AFLP, ISSR, chromosome number and POD isoenzyme phenotype to confirm the diversity of Apocynum venetum Linn. germplasm and their genetic relationship in this region, and thereby to set up the background for reasonable exploitation and utilization of Apocynum venetum Linn.. The results showed that:
1. The total diversity index, diversity index within population, diversity index among populations, genetic diversity within and among populations were 1.858, 0.862, 0.996, 46.39% and 53.61%, respectively, which derived from 16 phenotypic values including plant height, stem thickness, internode length, branch length, inflorescence length, pod length, seed length, leaf length and leaf width of Apocynum venetum Linn.populations originated from Tongliao, Baotou and Bayannaoer. There were big differences among the diversity index of morphological parameters in Apocynum venetum Linn.. The higher diversity index was observed in plant height, stem thickness, node number, internode length, branch length, thousand-seed weight, seed width, pod length, top inflorescence length, calyx length, stipule length, leaf length and leaf width, indicating that these were the major characteristics giving rise to the morphological variance and were readily affected by the environment.
2. The clustering of populations of Apocynum venetum Linn. originating from 3 regions of Inner Mongolia based on morphological properties showed that populations of Apocynum venetum Linn. from Tongliao was classified into one group while Apocynum venetum Linn. from Baotou and Bayannaoer belonged to another group, indicating that there was close relationship between Apocynum venetum Linn. populations from Baotou and Bayannaoer.
3. 5359 AFLP loci were found by DNA amplification with 45 Apocynum venetum Linn. populations from 3 regions, with 91.22% polymorphic loci. AFLP genetic diversity index, AFLP genetic diversity index within population, AFLP genetic diversity index among populations, differentiation coefficient within population and differentiation coefficient among populations were 1.286, 0.533, 0.753, 41.45% and 58.55%, respectively. The variance of Apocynum venetum Linn. germplasm was mainly derived from variance within population and among populations. Coefficient of difference in Apocynum venetum Linn. from 3 regions varied between 0.0042 and 0.819. Regarding coefficient of difference as a benchmark, most of Apocynum venetum Linn. populations from Tongliao region were sorted into one group while most of Apocynum venetum Linn. populations from Baotou and Bayannaoer were categorized to another group, showing that the relationship between Tongliao group and Baotou group was wide.
4. 65 ISSR loci, 55 polymorphic loci were observed by amplification using 6 ISSR primers. 84.62% were polymorphic loci. ISSR genetic diversity index, ISSR genetic diversity index within population, ISSR genetic diversity index among population and genetic differentiation coefficient were 0.424, 0.197, 0.227 and 53.54%, respectively. Regarding coefficient of difference as a benchmark, Apocynum venetum Linn. populations from Tongliao was classified into one group, while Apocynum venetum Linn. populations from Baotou and Bayannaoer came to another group, indicating there was close relationship between Apocynum venetum Linn. populations from Baotou and Bayannaoer.
5. 20 (2n =2x =20) chromosomes were observed in Apocynum venetum Linn. from Tongliao while 16(2n =2x =16) chromosomes in Apocynum venetum Linn. from Baotou and Bayannaoer. This result demonstrated that there was difference in chromosome number among Apocynum venetum Linn. populations from west to east of Inner Mongolia.
6. POD isoenzyme phenotypic zymograms at different organs (leaf, stem and root) showed some differences among Apocynum venetum Linn. populations originated from 3 different regions.
引文
1张磊,李惠琪,祁和英.阿克苏地区罗布麻资源的分布及利用[J].新疆农业科学,2003,40(3):172-174
2揭雨成,冷娟,许英.罗布麻生态特征与产业化研究进展[J].中国麻业,2001,23(3):34-37
3张广伦,钱学射,顾龚平.晋冀豫鲁罗布麻资源及栽培技术[J].中国野生植物资源,2005, 24(6):26-27,50
4刘起棠.罗布麻的过去与“罗布红麻"的将来[J].中国野生植物资源,2005,24(4):21-22
5张秀.罗布麻资源的开发与综合利用[J].中国野生植物,1991,(1):42-43
6赵秀芳,赵彦杰.罗布麻的开发利用价值及栽培技术[J].中国水土保持,2005,(10):42,45
7王宁,陈斌.柴达木盆地资源植物--罗布麻的开发利用[J].青海科技,2005,(6):15-17
8阿斯娅·曼力克,孙韬,塞里克·都曼,崔恒心.新疆大叶白麻资源及其开发利用前景[J].中国农业资源与区划,2003,24(3):27-30
9胡瑞林,钱学射,管启良,刘翠云.罗布麻叶和花的比较观察[J].中国野生植物资源,1999, 18(1):50-52
10张绍武,胡瑞林,钱学射.我国罗布麻分布区的地理区划[J].中国野生植物资源,2000,19 (4):20-22
11钱学射,张卫明,陈重明.罗布麻的民族植物学与资源开发利用[J].中国医学生物技术应用杂志,2002,3:15-21
12苏红文,马淼,李学禹.罗布麻和白麻不同居群植物的比较解剖学研究[J].西北植物学报1999,17(3):348-354
13初敬华,高晨光.罗布麻营养器官的解剖学研究[J].绥化师专学报, 1997,(2):99-100
14常兆丰.罗布麻资源的开发利用[J].林业科技开发,1994,(1):44-45.
15张秀玲.山东罗布麻的分布及开发利用[J].中国野生植物资源,2004,23(5):28-29.
16徐红.新疆罗布麻品质特点及开发[J].毛纺科技, 2005,6:37-40.
17喻春明.罗布麻的概况及开发利用前景[J].中国麻作,1996,18(3):40-41.
18胡瑞林,张绍武.罗布麻种子贮存研究[J].中国野生植物资源,1996,1:6-10
19周波.罗布麻解剖结构的研究[J].贵州工业大学学报(自然科学版), 2005,34(6):97-99
20周玲玲,陆嘉惠,吴玲,马淼.罗布麻花解剖结构的观察研究[J].石河子大学学报(自然科学版) ,2000,4(1):39-42
21何宗祥,除金萍,陈国璋.新疆罗布麻特征特性观察[J].中国麻作, 1997,19(3):21-23
22马骥,李俊祯,刘新民.罗布麻与大花罗布麻种子微结构特征[J].西北植物学报,2000,20(3):476-479
23胡瑞林,林木烂,董正钧.罗布麻生长发育规律的探讨[J].中国野生植物资源, 1998,17(3):35-49
24白璐,罗明柏,殷录成,王树廉,阿米娜.罗布红麻人工栽培技术研究简报[J].中国野生植物资源,2005,24(5):65,68
25沈法富,于元杰,尹承佾抗盐碱罗布麻DNA导入棉花的研究[J]棉花学报1995,7(1):18-21
26魏凌基,王咏星,徐海霞,马淼.罗布麻茎段的组织培养[J].植物生理学通讯, 2000,36(5):434
27陈彦云,曹君迈,李国旗,孟军.罗布麻离体培养及快繁技术的研究[J].生物技术2006,16(1):72-75
28刘风珍,尹承佾,陈章良.罗布麻基因文库的构建[J].山东农业大学学报,1995,26(1):8-12
29夏铭.遗传多样性研究进展[J].生态学杂志,1999,18(3):59-65
30施立明,贾旭.遗传多样性.中国的生物多样性[M].北京:科学出版社,1993 ,99-113
31解新明,云锦凤.植物遗传多样性及其检测方法[J].中国草地,2000,6 :51-59
32李俊清.植物遗传多样性及保护研究进展[J].植物研究,1998,18(2):227-242
33胡志昂.对我国遗传多样性研究的几点意见[J].生物多样性研究进展(首届全国生物多样性保护与持续利用研讨会论文集),中国科学技术出版社,1995
34 Fang D Q,Roose M I. Identificatoin of closely related citrus cultivars with inter-simple sequence repeat markers[J]. Theoretical and Applied Genetics,1997,95(3):408-417.
35易克.西瓜遗传图谱的构建及其重要农艺性状的基因定位[D].湖南农业大学,博士学位论文,2002
36翟文强,田清震,贾继增,董玉琛.哈密瓜杂交种纯度的AFLP指纹鉴定[J].园艺学报, 2002,29 (6): 587
37李惟基.遗传学[M].北京:中国农业大学出版社,2007
38肖海峻.鹅观草种质资源遗传多样性研究[D].中国农业科学院,博士学位论文,2007
39肖猛.濒危植物桃儿七的遗传多样性研究[D].四川大学,博士学位论文,2006
40王赞.柠条锦鸡儿遗传多样性研究[D].中国农业大学,博士学位论文,2006
41兰保祥,李立会,王辉.蒙古冰草居群遗传多样性研究[J].中国农业科学,2005,38(3):468-473.
42孙伟.马铃薯种质资源遗传多样性研究[D] .湖南农业大学,硕士学位论文,2006
43周连第.板栗种质资源遗传多样性研究[D] .中国农业大学,博士学位论文,2005
44邱英雄,黄爱军,傅承新.明党参的遗传多样性研究[J].植物分类学报,2003,8(2):111-120.
45胡宝忠,刘娣,胡国富,姜述君,张阿英.羊草遗传多样性的研究[J].植物生态学报,2001,25(1)83-89.
46罗建勋.云杉天然群体遗传多样性研究[D] .中国林业科学院,博士学位论文,2004
47蔚荣海.糯玉米种质资源遗传多样性研究[D].吉林农业大学,博士学位论文,2006
48 Merrell , D. J . (黄瑞复等译) .生态遗传学.北京:科学出版社, 1991.
49马三梅,王永飞,王得元.农作物分子遗传图谱的研究进展[J].干旱地区农业研究,2004,22(4):101-108
50李媛媛,沈金雄,王同华,傅廷栋,马朝芝.利用SRAP、SSR和AFLP标记构建甘蓝型油菜遗传连锁图谱[J].中国农业科学,2007,40(7):1118-1126
51 Cardional A J,Lee M,Sharopova N. Genetic mapping and analysis of quantitative trait loci for resistance to stalk tunneling by the European corn borer in maize[J]. Crop Sci,2001,41:835-845
52孙雁,冯锐,段利琴.杂交玉米及亲本种子碱溶蛋白电泳鉴定[J].种子,2000,(5):17-18
53 Agnieszka Gr?dzielewska. The genus Dasypyrum-part 2. Dasypyrum villosum-a wild species used in wheat improvement[J]. Euphytica,2006,152(3):441-454
54 Popov V N,Urbanovich O Yu,Kirichenko V V. Studying genetic diversity in inbred sunflower lines by RAPD and isozyme analyses[J]. Russian Journal of Genetics,2002,38(7):785-790
55 Charmet G,Balfourier F. Isozyme variation and species relationships in the genus Lolium L.(Ryegrasses,Graminaceae)[J]. Theoretical and Applied Genetics,1994,87(6):641-649
56 Seifu Tsegaye, Tesfaye Tesemma , Getachew Belay. Relationships among tetraploid wheat (Triticum turgidum L.) landrace populations revealed by isozyme markers and agronomic traits[J]. Theoretical and Applied Genetics,1996,93(4):600-605
57 Kishimoto N,Higo K,Higo H,Saito A. The coding sequence for rice seed catalase detects a locus different from that determined by isozyme analysis[J]. Theoretical and Applied Genetics,1994,87(6):625-626
58 Yamashita M,Abe J. Shimamoto-variation and differentiation of allelic frequencyds of isozyme loci in perennial ryegrasss(Lolium perenne)[J]. Journal of Japanese Society of Grassland Science,1993,38(4):459-468
59 Bouvier L,Guérif Ph,Djulbic M,Durel Ch E,Chevreau E,Lespinasse Y. Chromosome doubling of pear haploid plants and homozygosity assessment using isozyme and microsatellite markers[J]. Euphytica,2002,123(2):255-262
60 Fran?ois Balfourier,Jose Alberto Oliveira,Gilles Charmet,Enrique Arbones. Factorial regres- sion analysis of genotype by environment interaction in ryegrass populations,using both isozyme and climatic data as covariates[J]. Euphytica,1997,98(1-2):37-46
61 Sagnard F,Barberot C,Fady B. Structure of genetic diversity in Abies allba Mill,from south- western Aplps:multivariate analysis of adaptive and non-adaptive traits for conservation in France[J]. For. Ecol. Man.,2002,157:175-189
62吕秀兰,张光伦,龚荣高,汪志辉,曾秀丽,苟琳. 22个葡萄品种过氧化物同工酶研究[J].四川农业大学学报,2005,23(2):182-185
63于卓.小麦族禾草杂交后代农艺及分子细胞遗传学特性[D].内蒙古农业大学,博士学位论文,1999
64聂刘旺,张定成,张海军,张小爱,柳李旺,张克云.安徽产石蒜属植物三种酶同工酶的分析[J].生物学杂志,2003,20(2):27-29
65马艳红.几种小麦族禾草远缘杂交后代育性恢复研究[D].内蒙古农业大学,博士学位论文,2007
66张帆,万雪琴,潘光堂.玉米分子遗传图谱的构建[J].玉米科学,2006,14(3):6-9
67 Loutier S,Cappadocia M,Landry B.S. Analysis of RFLP mapping inaccuracy in Brassica napus L[J]. Genetics,1997,95:83-91
68贾继增.分子标记种质资源鉴定和分子标记育种[J].中国农业科学,1996,29(4):1-10
69辛业芸.分子标记技术在植物学研究中的应用[J].湖南农业科学,2002,(4):9-12
70米福贵.分子标记技术在植物育种中的应用[J].草原与草坪,2000,(2):3-7
71周延清. DNA分子标记技术在植物研究中的应用[M].北京:化学工业出版社,2005
72 Gardiner J M,Coe E H,Melia-Hancock S. Development of acore RFLP map in maize using an immortalized F2 population[J]. Genetics,1993,134:917-930
73 Tanksley S D,Nelson J C. Advanced backcross QTL analysis a method for the simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines[J]. Theor Appl Genet,1996,92:191-203
74 Pressois G,Albar L,Ahmadi N. Genetic basis and mapping of the resistance to rice yellow mottle virus II Evidence of a complementary epistasis between two QTLs[J]. Theor Appl Genet,1998,97:1155-1161
75 Jiang C J,Zeng Z B. Multiple triats analysis of genetic mapping for quantitative trait loci[J]. Genetics,1995,140:1111-1127
76 Laurie D A,Pratchett N,Bezant J H. RFLP mapping of five major genes and eight quantitative trait loci controlling flowering time in a winter×spring barley cross[J]. Genome,1995,38:575-585
77张海英.黄瓜重要抗病基因的分子标记研究及遗传图谱的构建[D].中国农业科学院,博士学位论文,2006
78叶少平.水稻分子图谱的构建和QTL定位研究[D].四川农业大学,博士学位论文,2004
79宛煜嵩.大豆遗传图谱的构建及若干农艺性状的QTL定位分析[D].中国农业科学院,博士学位论文,2002
80张德水,陈受宜. DNA分子标记、基因组作图及其在植物遗传育种上的应用[J].生物技术通报,1998,(5):15-22
81王和勇,陈敏,廖志华,孙敏. RFLP、RAPD、AFLP分子标记及其在植物生物技术中的应用[J].生物学杂志,1999,16(4):24-25
82 Welsh J,Mcclelland M. Fingerprinting genomes using PCR with arbitrary primer [J]. Nucleic. Acids. Res.,1990,18(24):7213-7218
83 Kojima T,Nagaoka T,Noda K. Genetic linkage map of ISSR and RAPD markers in Einkorn wheat in relation to that of RFLP markers[J]. Theoretical and Applied Genetics,1998,96:37-45
84曹必好,雷建军,孙秀东,陈国菊,孟成民.茄子RAPD分子标记图谱的构建[J].园艺学报,33(5):1092
85文晓鹏,庞晓明,邓秀新.刺梨及部分近缘种形态学性状和RAPD标记分析[J].园艺学报, 2003, 30(2):204-206.
86罗素兰,贺普超,周鹏,郑学勤.利用RAPD标记和种间杂交组合构建葡萄的分子标记连锁遗传图谱[ J].园艺学报,2001,28(1):68-70.
87陈书霞,王晓武,方智远,程智慧,孙培田.RAPD标记构建芥蓝×甘蓝分子标记连锁图[J].园艺学报,2002,29(3):229-232.
88左志锐,穆鼎,高俊平.百合遗传多样性及亲缘关系的RAPD分析[J].园艺学报,2005,32(3):468-472.
89张景荣,刘军.名贵茶花种质资源的RAPD分析[J].西北植物学报,2006,26(4):83
90李卫华,刘伟,尤明山,许杰,刘春雷,李保云,刘广田.利用多种SSR引物构建小麦遗传连锁图谱及其多态性分析[J].麦类作物学报,2007,27(7):1-6
91张洁夫,戚存扣,栗根义,浦惠明,陈松,陈新军,高建芹,陈锋,顾慧,傅寿仲.甘蓝型油菜遗传图谱构建与无花瓣性状QTL定位[J].作物学报,2007,33(8):1246-1254
92郑轶琦,李作洲,黄宏文.猕猴桃品种SSR分析的初步研究[J].武汉植物学研究, 2003,21(5): 444-44
93 Hokanson S C.Microsatelite (SSR) markers revealgenetic identies diversity and relationship inMalus x domestic Borkh.Coresubet collection[J]. Theoretical and Applied Genetics,1998,97:671-680
94罗玉兰,张冬梅,杨娅.红刺玫DNA提取及SSR引物筛选[C].园林科技,2006:148
95辛业芸,张展,熊易平,袁隆平.应用SSR分子标记鉴定超级杂交水稻组合及其纯度[J].中国水稻科学,2005,19(2):95-100
96李晓辉,李新海,李文华,王振华,马凤鸣,袁力行,张世煌. SSR标记技术在玉米杂交种种子纯度测定中的应用[J].作物学报,2003,29(1):63-68
97 Vos P,Hogers R,Bleeker M,et al. AFLP:a new technique for DNA fingerprinting [J]. Nucleic Acids Research,1995,23(21):4407-4414
98高蓝,李浩明.分子标记在番茄遗传育种研究中的应用[J].遗传学报,2003,25(3):361-366
99张春庆,贾继增.利用AFLP技术鉴定玉米自交系及杂交种[J].山东农业大学学报, 2003, 34 (3): 427~430
100 Pereira M G,Lee M,Whitekus R. Construction of an RFLP map in sorghum and comparative mapping in maize[J]. Genome,1994,37:236-243
101 Saghai Maroof MA,Biyashey RM. Extraordinarily polymirphic microsatellite DNA in Garlet Species diversity chromosomal locations and population dynamics Proc[J]. Natl Acad Sci,1992,91:5466-5470
102 Barrett B A,kidwell k k. AFLP-based genetic diversity assessment among wheat cultivars from the Pacific Northwest[J]. Crop Science,1998,38(5):1261-1271
103张镝,丁毅.基于AFLP标记的中国西藏近缘野生大麦遗传多样性分析[J].遗传,2007, 29(6):725-730
104 Hongtrkul V,Huestis G M,Knapp S. Amplified fragment length polyrnorphisms as a tool for DNA fingerprinting sunflower germplasm:Genetic diversity among oilseed inbred lines[J]. Theoretical and Applied Genetics,1994,89:9-13
105彭宏祥,曹辉庆,朱建华,廖惠红,黄凤珠,李江舟,梁文.利用AFLP分子标记对广西荔枝优稀种质遗传多样性及分类研究[J].西南农业学报,2006,19(1):108-111
106 Tignon M,Kettmann R. AFLP:use for the identification of apple cultivars and mutants[J]. Acta Hort,2000,512:219-226
107杨佳,李晓东,李新伟,史全芬,李建强.华中特有珍稀植物裸芸香的AFLP遗传多样性分析[J].武汉植物学研究,2007,25(3):226-234
108郑红梅,呼天明,王佺珍，张国云，宋江湖，胡晓宁.嵩草AFLP分析体系的建立及引物筛选[J].西北农林科技大学学报,2008,36(3):33-38
109李海生.ISSR分子标记技术及其在植物遗传多样性分析中的应用[J].生物学通报,2004,39(2):19-21
110范彦,曾兵,张新全,马啸.中国野生鸭茅遗传多样性的ISSR研究[J].草业学报,2006,15(5):103-108.
111 Prevost A,Wilkinson M J. A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars[J]. Theoretical and Applied Genetics,1999,98(1):107-112.
112王金花,罗文永,陈建伟,刘元风,吴秋花,李晓方.应用SSR和ISSR标记分析栽培香稻品种的遗传多样性[J].分子植物育种,2005, 3(1):37-42.
113夏婧,郭友好.邓氏马先蒿遗传多样性的ISSR分析[J].武汉植物学研究,2006,24 (6):565-568
114祁建民,王涛,陈顺辉,周东新,方平平,陶爱芬,梁景霞,吴为人.部分烟草种质遗传多样性与亲缘关系的ISSR标记分析[J].作物学报,2006,32(3):373-378.
115 Li A,Ge S.Genetic variation and clonal diversity of Psammochloa villosa (Poaceae) Detected by ISSR Markers [J].Annals Botany,2001,87:585-590.
116王晓波,马传喜,何克勤,司红起,张业伦.小麦2D染色体上多酚氧化酶(PPO)基因STS标记的开发与应用[J].中国农业科学,2008,41(6):1583-1590
117孙华钦,田洁,郑家奎,蒋开锋,罗科.糯稻和非糯稻蜡质基因的新分子标记[J].应用与环境生物学报2006,12(4):461-463
118 Devos K M,Millan T,Gale M D. Comparative RFLP maps of homoeologous group 2 chromoso- mes of wheat and barley[J]. Theoretical and Applied Genetics,1993,85:784-792
119 Li H B,Wang J,Liu A M. Genetic basis of low temperature sensitive sterility in indica japonica hybrids of rice as determined by RFLP analysis[J]. Theoretical and Applied Genetics,1997,95:1092-1097
120方荣,陈学军,缪南生.遗传标记技术及其在辣椒系统发育中的应用研究[J].江西农业学报, 2006,18(2):8-14
121 Bezawada C,Saha S,Jenkins J N. SSR markers associated with root knot nematode resistance genes in cotton[J]. Journal of Cotton Science,2003,7:179-184
122于拴仓.大白菜分子遗传图谱的构建及重要农艺性状的QTL定位[D].中国农业科学院,博士学位论文,2003
123 Guo W Z,ZhangT Z, Pan J J. Identification of RAPD marker linked with fertility-restoring gene of cytoplasmic male sterile lines in upland cotton[J]. Chinese SciBulletin,1998,43:52-54
124 Haanstra J P W,Wye C,Verbakel H. An integrated high density RFLP-AFLP map of tomato based on two Lycopersicon esculetumΧL. pennellii F2 populations[J]. Theoretical and Applied Genetics,1999,99:254-271
125杨玉珍,彭方仁.遗传标记及其在林木研究中的应用[J].生物技术通报,2006,17(5):788-791
126李景欣.内蒙古冰草种质资源遗传多样性研究[D].内蒙古农业大学,博士学位论文,2005
127马缘生,谭富娟,李灵芝,柴建方,范传珠,马榕荫,周红立,周涛.异花授粉作物大白菜和荞麦基因库种子繁殖技术研究[J].中国农业科学,2000,33(2):16-22.
128 Arora R K. Plant exploration and germplasm collection[C].In:Paroda R.S.and Arora R.K.(de.) Plant germplasm Resources Conservation and Management,published by IBPGR,New Delhi,India.1991.
129 Eujayl I,Baum M. A genetic linkage map of lentil(Lens culinaris)[J].Theoretical and Applied Genetics,1998,97:83-89.
130沈金雄,郭庆元,张秀荣,赵应忠,冯祥远,陈和兴,伍晓明.中国芝麻种质资源的聚类分析[J].华中农业大学学报,1995,14(6)532-536.
131樊叶杨,庄杰云,李强.水稻株高QTL分析及其与产量QTL的关系[J].作物学报,2001,27 (6):915-992.
132 Edwards A,Civitello A,Hammond H A,Caskey C T. DNA typing and genetic mapping with trimeric and tetrameric tandem repeats [J].Genet.,1991,97:746-765.
133付福友.甘蓝型油菜遗传图谱的构建和品质相关性状的QTL分析[D].西南大学,博士学位论文,2007
134聂以春,左开井,张献龙.RAPD标记在棉属种间杂种后代检测中的应用[J].中国农业科学,2000,33(5):25-29.
135 Ge X J,Sun M. Reproductive biology and genetic diversity of a cryptoviviparous mangrove Aegiceras corniculatum (Myrsinaceae) us ingallozyme and inter-simple sequence repeat(ISSR)analysis[J]. Molecular Ecology,1999,8:2061-2069.
136 Falconer D S. Introduction to quantitative genetics(2ed.)[M]. Longmn Inc.,New York,1981.
137 Nei M,Li W H. Mathematical model for studying genetic variation in terms of restriction endonucleases[J]. Proc.,Natl.,Acad ,Sci.,USA,1979,76:5269-5273.
138 Nei M. Molecular evolutionary genetics[M].Columbia Univ. Press, New York.1987.
139彭秀玲.基因工程实验技术[M].湖南科学技术出版社,1997
140 PERani J. SYN-TAX-pc-supplement 3:Mscintosh verion[M]. Abstr.Bot,1990,14:23-29.
141 Falconer D.S. Introduction to quantitative genetics(3ed.)[M]. Longman scientific and Technical, New York,USA,1989.
142徐云碧,朱立煌.分子数量遗传学[M].北京:中国农业出版社,1994
143云锦凤,王照兰,杜建才.加拿大披碱草与老芒麦种间杂交及F1代细胞学分析[J].中国草地,1997,(1):32-35.
144 Prabhu R.R,Webb D,Jessen H,Luk S,Smith S,Gresshoff P.M.. Geneticrelatedness among soubean genotypes using DNA amplification fingerprinting,RFLP and Pedigree[J].Crop Sci.,1997,37:1590-1595.
145 Huang J C,Sum M. Genetic diversity and relationship of sweetpotato and its wild relatives in Ipomoea series Batatas (Convolvulaceae) as revealed by inter-simple sequence repeat(ISSR) and restriction analysis of chloroplast DNA[J]. Theoretical and Applied Genetics,2000,100:1050-1060.
146 Liu C J. Geographical distribution of genetic in stylosanthes scabra revealed by RAPD analysis[J]. Euphytica,1997,98:21-27.
147胡志昂.遗传多样性的定义.研究新进展和新概念.生物多样性与人类未来[M].中国林业出版社,北京.1998.
148汪殿蓓,暨淑仪,陈飞鹏.植物群落物种多样性研究综述[J].生态学杂志,2001,20(4):55-60.
149 Dnaiel Z V,Dnaiel P. Patterns of morphological variation and diversity of Coocs nucifera (arecaecae) in Mexico[J]. American Journal of Botany,1998,85(6):855-865.
150罗建勋,顾万春..云杉天然群体种实性状变异研究[J].西北农林科技大学学报,2004,32(8):60-66.
151李斌,顾万春,卢宝明.白皮松天然群体种实性状表型多样性研究[J].生物多样性,2002,10 (2):181-188.
152 Patricia C G,Enriqu E L ,Filogonio M P.. Patterns of morphological variation,diversity,and domestication of wild and cultivated populations of Agave in Yucatan,Mexico[J]. American Journal of Botany ,1996,83(8):1069-1082.
153王小平,刘晶岚,王九龄,刘春江.白皮松种子及球果形态特征的地理变异[J].北京林业大学学报,1998,20(3):25-31.
154孟庆杰,王光全,董绍峰.桃叶片组织解剖结构特征与其抗旱性关系的研究[J].干旱地区农业研究,2004,22(3):123-126.
155王志峰,孙日飞.山东省黄瓜地方黄瓜品种的遗传亲缘关系AFLP研究[J].园艺学报,2004,31(1):103-105.
156杜娟,马小军,李学东.半夏不同种质资源AFLP指纹系谱分析及其应用[J].中国中药杂志,2006,31(1):30-33.
157房经贵,乔玉山,章镇.AFLP在芒果品种鉴定中的应用[J].广西植物,2001,21(3):281-283.
158黄建安,李家贤,黄意欢.茶树品种资源遗传多样性的AFLP研究[J].园艺学报,2006,33(2):317-322.
159杨朝东,王健,张俊卫.梅花不同样本间亲缘关系的AFLP初步分析[J].中国农业科学,2005,38(10):2084-2089.
160石胜友,成明昊,梁国鲁.变叶海棠遗传多样性的AFLP分析[J].园艺学报,2006,33(2):381-384.
161于歆,徐昌德.双丰系列甜菜品种AFLP亲缘关系及系谱分析分子植物育种[J].分子植物育种,2004,12(2):229-234.
162于卓,马艳红,李小雷,周亚星,李晓宇,李造哲.加拿大披碱草与披碱草、圆柱披碱草2个种间杂种F1及其亲本的AFLP分析[J].草地学报,2008,16(5):431-435.
163钱韦,葛颂,洪德元等.采用RAPD和ISSR标记探讨中国庆粒野生稻的遗传多样性[J].植物学报,2000,42(7):741-750.
164唐启义,冯明光.实用统计分析及其DPS数据处理系统[M].北京:科学出版社,2002.
165李鸣,谭裕模,李杨瑞,李容柏,高国庆.甘蔗(Saccharum officinarum L.)品种遗传差异的AFLP分子标记分析[J].作物学报,2004,30(10):1008-1013
166翟文强,田清震,贾继增,董玉琛.哈密瓜杂交种纯度的AFLP指纹鉴定[J].园艺学报2002, 29 (6): 586-587
167田雷,曹鸣庆,王辉,郭晶心,周乃元,孙江,贾希海. AFLP标记技术在鉴定甘蓝种子真实性及品种纯度中的应用[J].生物技术通报,2001,(3):38-40,44.
168 Zietkiewicz E , Rafalski A , Labuda D. Genome fingerprinting by simple sequence repeat(ISSR)-anchoredpolymerarase chain reaction amplification[J]. Genomics,1994,20:176-183
169 KojimaT,NagalkaT,Noda K. Genetic linkage map of ISSR and RAPD markers in Einkorn Wheat in relation to that of RFLP markers[J]. Theoretical and Applied Genetics,1998,96:37-45.
170赵茹,程舟,陆伟峰,卢宝荣.基于分子标记的野生大豆居群遗传多样性估算与取样策略[J].科学通讯,2006,51(9):1042-1048
171黄文霞,何觉民,朱宏波.蓖麻种质资源遗传多样性的ISSR分析[J].西北农业学报,2008 ,17(1):182-184,187
172褚云霞,汤庚国,张永春,靖相密.蜡梅ISSR反应体系的优化[J].北方园艺,2007,(10):161-163
173罗淑萍,曾斌,孙晋科,李疆.野扁桃ISSR -PCR反应体系的优化[J].经济林研究2007, 25(3):1-5
174周延清,景建洲,李振勇,浩健,贾敬芬.怀地黄ISSR扩增条件优化的研究[J].西北植物学报2004,24(1):6-11
175沈永宝,施季森,赵洪亮.利用ISSR DNA标记鉴定主要银杏栽培品种.林业科学,2005,41(1):202-204
176李国珍.染色体及其研究方法[M].北京:科学出版社,1985:303-314.
177李懋学,张赞平.作物染色体及其研究技术[M].北京:中国农业出版社,1996:307-311.
178李贵全.细胞学研究基础[M].北京:中国林业出版社,2001:6-8.
179黎中明,林文君.细胞遗传学[M].成都:四川大学出版社,1988:44-49.
180于卓,云锦凤,李造哲.加拿大披碱草与野大麦及其属间杂种细胞遗传学研究[A].见:中国国际草业发展大会暨中国草原学会第六届代表大会学术及产业研讨会论文集[C].北京:中国农业出版社,2002.
181苏东梅.柿属君迁子和湖南九个乡土品种过氧化物酶同工酶的研究[J],武汉植物学研究,1991,9(3):253-258.
182梁宏伟,刘姝,陈发菊,熊丹.银鹊树种子萌发过程中的过氧化物酶和酯酶同工酶的变化[J].种子,2006,25(5):38-40.
183葛颂.同工酶和植物进化生物学研究.植物进化生物学[M].武汉:武汉大学出版社1994,153- 208.
184张学科,毛子军,宋红,孟斌.五种落叶松遗传关系的等位酶分析[J].植物研究,2002,22(2):224-230.
185蓝登明,何文兴. 8个沙棘品系间等位酶水平遗传多样性研究[J].沙棘,2003,16(4):11-15.
186全妙华,陈东明,付明,姚元枝.四棱豆过氧化物酶同工酶的研究[J].中国农学通报,2006,22(4):203-206
187马云峰,薛瑰玲,尚富德,大白菜幼苗过氧化物同工酶的研究[J].河南大学学报,2006,3,23(1):82-84.
188沙伟,孟凡玲,冯昌军. 10个苜蓿品种过氧化物同工酶POD分析[J].北方园艺,2007,(1):150-152.
189陈燕珍,胡丽莲,陶毅明,梁杨琳.不同生育期菊芋叶片过氧化物同工酶表达特点的研究[J].生物学杂志,2007,2(24):25-46.
190 Joseph,L.S.and Boouwkamp,J.C..Karyomorphology of several species of Phaseolusand Vigna[J].Cytologia,1978,43:595-600.
191解新明,云锦凤,赵冰,卢小良,李秉滔.蒙古冰草遗传多样性的等位酶分析[J].草业科学, 2001,18(6):6-11.
192闫忠业,伊凯,李作轩,刘志,王冬梅,杨峰.富士系苹果过氧化物同工酶研究[J].沈阳农业大学学报,2006,37(4):578-581.
193陈艳秋,李玉梅.黑木耳菌株氧化物同工酶的研究[J].吉林农业大学学报,2005, 27(1): 26-28.
194王中仁.植物等位酶分析[M].科学出版社,北京,1996.
195孔祥祯,曲延英,丁勇,何宗祥,楚金平.新疆罗布麻属和白麻属植物的染色体数目[J].八一农学院学报,1993,16(4):38-39.
196陈彦云,曹君迈,李国旗,任辉丽,谢敏.罗布麻染色体核型分析[J].北方园艺,2008,(1):200-202
197张龙翔,张庭芳,李令媛.生化试验方法和技术[M].高等教育出版社,1981,11:119-123.
198张相歧.番茄属四个钟的过氧化物酶同工酶的分析[J].植物研究,1987,7(4):131-136.
2揭雨成,冷娟,许英.罗布麻生态特征与产业化研究进展[J].中国麻业,2001,23(3):34-37
3张广伦,钱学射,顾龚平.晋冀豫鲁罗布麻资源及栽培技术[J].中国野生植物资源,2005, 24(6):26-27,50
4刘起棠.罗布麻的过去与“罗布红麻"的将来[J].中国野生植物资源,2005,24(4):21-22
5张秀.罗布麻资源的开发与综合利用[J].中国野生植物,1991,(1):42-43
6赵秀芳,赵彦杰.罗布麻的开发利用价值及栽培技术[J].中国水土保持,2005,(10):42,45
7王宁,陈斌.柴达木盆地资源植物--罗布麻的开发利用[J].青海科技,2005,(6):15-17
8阿斯娅·曼力克,孙韬,塞里克·都曼,崔恒心.新疆大叶白麻资源及其开发利用前景[J].中国农业资源与区划,2003,24(3):27-30
9胡瑞林,钱学射,管启良,刘翠云.罗布麻叶和花的比较观察[J].中国野生植物资源,1999, 18(1):50-52
10张绍武,胡瑞林,钱学射.我国罗布麻分布区的地理区划[J].中国野生植物资源,2000,19 (4):20-22
11钱学射,张卫明,陈重明.罗布麻的民族植物学与资源开发利用[J].中国医学生物技术应用杂志,2002,3:15-21
12苏红文,马淼,李学禹.罗布麻和白麻不同居群植物的比较解剖学研究[J].西北植物学报1999,17(3):348-354
13初敬华,高晨光.罗布麻营养器官的解剖学研究[J].绥化师专学报, 1997,(2):99-100
14常兆丰.罗布麻资源的开发利用[J].林业科技开发,1994,(1):44-45.
15张秀玲.山东罗布麻的分布及开发利用[J].中国野生植物资源,2004,23(5):28-29.
16徐红.新疆罗布麻品质特点及开发[J].毛纺科技, 2005,6:37-40.
17喻春明.罗布麻的概况及开发利用前景[J].中国麻作,1996,18(3):40-41.
18胡瑞林,张绍武.罗布麻种子贮存研究[J].中国野生植物资源,1996,1:6-10
19周波.罗布麻解剖结构的研究[J].贵州工业大学学报(自然科学版), 2005,34(6):97-99
20周玲玲,陆嘉惠,吴玲,马淼.罗布麻花解剖结构的观察研究[J].石河子大学学报(自然科学版) ,2000,4(1):39-42
21何宗祥,除金萍,陈国璋.新疆罗布麻特征特性观察[J].中国麻作, 1997,19(3):21-23
22马骥,李俊祯,刘新民.罗布麻与大花罗布麻种子微结构特征[J].西北植物学报,2000,20(3):476-479
23胡瑞林,林木烂,董正钧.罗布麻生长发育规律的探讨[J].中国野生植物资源, 1998,17(3):35-49
24白璐,罗明柏,殷录成,王树廉,阿米娜.罗布红麻人工栽培技术研究简报[J].中国野生植物资源,2005,24(5):65,68
25沈法富,于元杰,尹承佾抗盐碱罗布麻DNA导入棉花的研究[J]棉花学报1995,7(1):18-21
26魏凌基,王咏星,徐海霞,马淼.罗布麻茎段的组织培养[J].植物生理学通讯, 2000,36(5):434
27陈彦云,曹君迈,李国旗,孟军.罗布麻离体培养及快繁技术的研究[J].生物技术2006,16(1):72-75
28刘风珍,尹承佾,陈章良.罗布麻基因文库的构建[J].山东农业大学学报,1995,26(1):8-12
29夏铭.遗传多样性研究进展[J].生态学杂志,1999,18(3):59-65
30施立明,贾旭.遗传多样性.中国的生物多样性[M].北京:科学出版社,1993 ,99-113
31解新明,云锦凤.植物遗传多样性及其检测方法[J].中国草地,2000,6 :51-59
32李俊清.植物遗传多样性及保护研究进展[J].植物研究,1998,18(2):227-242
33胡志昂.对我国遗传多样性研究的几点意见[J].生物多样性研究进展(首届全国生物多样性保护与持续利用研讨会论文集),中国科学技术出版社,1995
34 Fang D Q,Roose M I. Identificatoin of closely related citrus cultivars with inter-simple sequence repeat markers[J]. Theoretical and Applied Genetics,1997,95(3):408-417.
35易克.西瓜遗传图谱的构建及其重要农艺性状的基因定位[D].湖南农业大学,博士学位论文,2002
36翟文强,田清震,贾继增,董玉琛.哈密瓜杂交种纯度的AFLP指纹鉴定[J].园艺学报, 2002,29 (6): 587
37李惟基.遗传学[M].北京:中国农业大学出版社,2007
38肖海峻.鹅观草种质资源遗传多样性研究[D].中国农业科学院,博士学位论文,2007
39肖猛.濒危植物桃儿七的遗传多样性研究[D].四川大学,博士学位论文,2006
40王赞.柠条锦鸡儿遗传多样性研究[D].中国农业大学,博士学位论文,2006
41兰保祥,李立会,王辉.蒙古冰草居群遗传多样性研究[J].中国农业科学,2005,38(3):468-473.
42孙伟.马铃薯种质资源遗传多样性研究[D] .湖南农业大学,硕士学位论文,2006
43周连第.板栗种质资源遗传多样性研究[D] .中国农业大学,博士学位论文,2005
44邱英雄,黄爱军,傅承新.明党参的遗传多样性研究[J].植物分类学报,2003,8(2):111-120.
45胡宝忠,刘娣,胡国富,姜述君,张阿英.羊草遗传多样性的研究[J].植物生态学报,2001,25(1)83-89.
46罗建勋.云杉天然群体遗传多样性研究[D] .中国林业科学院,博士学位论文,2004
47蔚荣海.糯玉米种质资源遗传多样性研究[D].吉林农业大学,博士学位论文,2006
48 Merrell , D. J . (黄瑞复等译) .生态遗传学.北京:科学出版社, 1991.
49马三梅,王永飞,王得元.农作物分子遗传图谱的研究进展[J].干旱地区农业研究,2004,22(4):101-108
50李媛媛,沈金雄,王同华,傅廷栋,马朝芝.利用SRAP、SSR和AFLP标记构建甘蓝型油菜遗传连锁图谱[J].中国农业科学,2007,40(7):1118-1126
51 Cardional A J,Lee M,Sharopova N. Genetic mapping and analysis of quantitative trait loci for resistance to stalk tunneling by the European corn borer in maize[J]. Crop Sci,2001,41:835-845
52孙雁,冯锐,段利琴.杂交玉米及亲本种子碱溶蛋白电泳鉴定[J].种子,2000,(5):17-18
53 Agnieszka Gr?dzielewska. The genus Dasypyrum-part 2. Dasypyrum villosum-a wild species used in wheat improvement[J]. Euphytica,2006,152(3):441-454
54 Popov V N,Urbanovich O Yu,Kirichenko V V. Studying genetic diversity in inbred sunflower lines by RAPD and isozyme analyses[J]. Russian Journal of Genetics,2002,38(7):785-790
55 Charmet G,Balfourier F. Isozyme variation and species relationships in the genus Lolium L.(Ryegrasses,Graminaceae)[J]. Theoretical and Applied Genetics,1994,87(6):641-649
56 Seifu Tsegaye, Tesfaye Tesemma , Getachew Belay. Relationships among tetraploid wheat (Triticum turgidum L.) landrace populations revealed by isozyme markers and agronomic traits[J]. Theoretical and Applied Genetics,1996,93(4):600-605
57 Kishimoto N,Higo K,Higo H,Saito A. The coding sequence for rice seed catalase detects a locus different from that determined by isozyme analysis[J]. Theoretical and Applied Genetics,1994,87(6):625-626
58 Yamashita M,Abe J. Shimamoto-variation and differentiation of allelic frequencyds of isozyme loci in perennial ryegrasss(Lolium perenne)[J]. Journal of Japanese Society of Grassland Science,1993,38(4):459-468
59 Bouvier L,Guérif Ph,Djulbic M,Durel Ch E,Chevreau E,Lespinasse Y. Chromosome doubling of pear haploid plants and homozygosity assessment using isozyme and microsatellite markers[J]. Euphytica,2002,123(2):255-262
60 Fran?ois Balfourier,Jose Alberto Oliveira,Gilles Charmet,Enrique Arbones. Factorial regres- sion analysis of genotype by environment interaction in ryegrass populations,using both isozyme and climatic data as covariates[J]. Euphytica,1997,98(1-2):37-46
61 Sagnard F,Barberot C,Fady B. Structure of genetic diversity in Abies allba Mill,from south- western Aplps:multivariate analysis of adaptive and non-adaptive traits for conservation in France[J]. For. Ecol. Man.,2002,157:175-189
62吕秀兰,张光伦,龚荣高,汪志辉,曾秀丽,苟琳. 22个葡萄品种过氧化物同工酶研究[J].四川农业大学学报,2005,23(2):182-185
63于卓.小麦族禾草杂交后代农艺及分子细胞遗传学特性[D].内蒙古农业大学,博士学位论文,1999
64聂刘旺,张定成,张海军,张小爱,柳李旺,张克云.安徽产石蒜属植物三种酶同工酶的分析[J].生物学杂志,2003,20(2):27-29
65马艳红.几种小麦族禾草远缘杂交后代育性恢复研究[D].内蒙古农业大学,博士学位论文,2007
66张帆,万雪琴,潘光堂.玉米分子遗传图谱的构建[J].玉米科学,2006,14(3):6-9
67 Loutier S,Cappadocia M,Landry B.S. Analysis of RFLP mapping inaccuracy in Brassica napus L[J]. Genetics,1997,95:83-91
68贾继增.分子标记种质资源鉴定和分子标记育种[J].中国农业科学,1996,29(4):1-10
69辛业芸.分子标记技术在植物学研究中的应用[J].湖南农业科学,2002,(4):9-12
70米福贵.分子标记技术在植物育种中的应用[J].草原与草坪,2000,(2):3-7
71周延清. DNA分子标记技术在植物研究中的应用[M].北京:化学工业出版社,2005
72 Gardiner J M,Coe E H,Melia-Hancock S. Development of acore RFLP map in maize using an immortalized F2 population[J]. Genetics,1993,134:917-930
73 Tanksley S D,Nelson J C. Advanced backcross QTL analysis a method for the simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines[J]. Theor Appl Genet,1996,92:191-203
74 Pressois G,Albar L,Ahmadi N. Genetic basis and mapping of the resistance to rice yellow mottle virus II Evidence of a complementary epistasis between two QTLs[J]. Theor Appl Genet,1998,97:1155-1161
75 Jiang C J,Zeng Z B. Multiple triats analysis of genetic mapping for quantitative trait loci[J]. Genetics,1995,140:1111-1127
76 Laurie D A,Pratchett N,Bezant J H. RFLP mapping of five major genes and eight quantitative trait loci controlling flowering time in a winter×spring barley cross[J]. Genome,1995,38:575-585
77张海英.黄瓜重要抗病基因的分子标记研究及遗传图谱的构建[D].中国农业科学院,博士学位论文,2006
78叶少平.水稻分子图谱的构建和QTL定位研究[D].四川农业大学,博士学位论文,2004
79宛煜嵩.大豆遗传图谱的构建及若干农艺性状的QTL定位分析[D].中国农业科学院,博士学位论文,2002
80张德水,陈受宜. DNA分子标记、基因组作图及其在植物遗传育种上的应用[J].生物技术通报,1998,(5):15-22
81王和勇,陈敏,廖志华,孙敏. RFLP、RAPD、AFLP分子标记及其在植物生物技术中的应用[J].生物学杂志,1999,16(4):24-25
82 Welsh J,Mcclelland M. Fingerprinting genomes using PCR with arbitrary primer [J]. Nucleic. Acids. Res.,1990,18(24):7213-7218
83 Kojima T,Nagaoka T,Noda K. Genetic linkage map of ISSR and RAPD markers in Einkorn wheat in relation to that of RFLP markers[J]. Theoretical and Applied Genetics,1998,96:37-45
84曹必好,雷建军,孙秀东,陈国菊,孟成民.茄子RAPD分子标记图谱的构建[J].园艺学报,33(5):1092
85文晓鹏,庞晓明,邓秀新.刺梨及部分近缘种形态学性状和RAPD标记分析[J].园艺学报, 2003, 30(2):204-206.
86罗素兰,贺普超,周鹏,郑学勤.利用RAPD标记和种间杂交组合构建葡萄的分子标记连锁遗传图谱[ J].园艺学报,2001,28(1):68-70.
87陈书霞,王晓武,方智远,程智慧,孙培田.RAPD标记构建芥蓝×甘蓝分子标记连锁图[J].园艺学报,2002,29(3):229-232.
88左志锐,穆鼎,高俊平.百合遗传多样性及亲缘关系的RAPD分析[J].园艺学报,2005,32(3):468-472.
89张景荣,刘军.名贵茶花种质资源的RAPD分析[J].西北植物学报,2006,26(4):83
90李卫华,刘伟,尤明山,许杰,刘春雷,李保云,刘广田.利用多种SSR引物构建小麦遗传连锁图谱及其多态性分析[J].麦类作物学报,2007,27(7):1-6
91张洁夫,戚存扣,栗根义,浦惠明,陈松,陈新军,高建芹,陈锋,顾慧,傅寿仲.甘蓝型油菜遗传图谱构建与无花瓣性状QTL定位[J].作物学报,2007,33(8):1246-1254
92郑轶琦,李作洲,黄宏文.猕猴桃品种SSR分析的初步研究[J].武汉植物学研究, 2003,21(5): 444-44
93 Hokanson S C.Microsatelite (SSR) markers revealgenetic identies diversity and relationship inMalus x domestic Borkh.Coresubet collection[J]. Theoretical and Applied Genetics,1998,97:671-680
94罗玉兰,张冬梅,杨娅.红刺玫DNA提取及SSR引物筛选[C].园林科技,2006:148
95辛业芸,张展,熊易平,袁隆平.应用SSR分子标记鉴定超级杂交水稻组合及其纯度[J].中国水稻科学,2005,19(2):95-100
96李晓辉,李新海,李文华,王振华,马凤鸣,袁力行,张世煌. SSR标记技术在玉米杂交种种子纯度测定中的应用[J].作物学报,2003,29(1):63-68
97 Vos P,Hogers R,Bleeker M,et al. AFLP:a new technique for DNA fingerprinting [J]. Nucleic Acids Research,1995,23(21):4407-4414
98高蓝,李浩明.分子标记在番茄遗传育种研究中的应用[J].遗传学报,2003,25(3):361-366
99张春庆,贾继增.利用AFLP技术鉴定玉米自交系及杂交种[J].山东农业大学学报, 2003, 34 (3): 427~430
100 Pereira M G,Lee M,Whitekus R. Construction of an RFLP map in sorghum and comparative mapping in maize[J]. Genome,1994,37:236-243
101 Saghai Maroof MA,Biyashey RM. Extraordinarily polymirphic microsatellite DNA in Garlet Species diversity chromosomal locations and population dynamics Proc[J]. Natl Acad Sci,1992,91:5466-5470
102 Barrett B A,kidwell k k. AFLP-based genetic diversity assessment among wheat cultivars from the Pacific Northwest[J]. Crop Science,1998,38(5):1261-1271
103张镝,丁毅.基于AFLP标记的中国西藏近缘野生大麦遗传多样性分析[J].遗传,2007, 29(6):725-730
104 Hongtrkul V,Huestis G M,Knapp S. Amplified fragment length polyrnorphisms as a tool for DNA fingerprinting sunflower germplasm:Genetic diversity among oilseed inbred lines[J]. Theoretical and Applied Genetics,1994,89:9-13
105彭宏祥,曹辉庆,朱建华,廖惠红,黄凤珠,李江舟,梁文.利用AFLP分子标记对广西荔枝优稀种质遗传多样性及分类研究[J].西南农业学报,2006,19(1):108-111
106 Tignon M,Kettmann R. AFLP:use for the identification of apple cultivars and mutants[J]. Acta Hort,2000,512:219-226
107杨佳,李晓东,李新伟,史全芬,李建强.华中特有珍稀植物裸芸香的AFLP遗传多样性分析[J].武汉植物学研究,2007,25(3):226-234
108郑红梅,呼天明,王佺珍，张国云，宋江湖，胡晓宁.嵩草AFLP分析体系的建立及引物筛选[J].西北农林科技大学学报,2008,36(3):33-38
109李海生.ISSR分子标记技术及其在植物遗传多样性分析中的应用[J].生物学通报,2004,39(2):19-21
110范彦,曾兵,张新全,马啸.中国野生鸭茅遗传多样性的ISSR研究[J].草业学报,2006,15(5):103-108.
111 Prevost A,Wilkinson M J. A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars[J]. Theoretical and Applied Genetics,1999,98(1):107-112.
112王金花,罗文永,陈建伟,刘元风,吴秋花,李晓方.应用SSR和ISSR标记分析栽培香稻品种的遗传多样性[J].分子植物育种,2005, 3(1):37-42.
113夏婧,郭友好.邓氏马先蒿遗传多样性的ISSR分析[J].武汉植物学研究,2006,24 (6):565-568
114祁建民,王涛,陈顺辉,周东新,方平平,陶爱芬,梁景霞,吴为人.部分烟草种质遗传多样性与亲缘关系的ISSR标记分析[J].作物学报,2006,32(3):373-378.
115 Li A,Ge S.Genetic variation and clonal diversity of Psammochloa villosa (Poaceae) Detected by ISSR Markers [J].Annals Botany,2001,87:585-590.
116王晓波,马传喜,何克勤,司红起,张业伦.小麦2D染色体上多酚氧化酶(PPO)基因STS标记的开发与应用[J].中国农业科学,2008,41(6):1583-1590
117孙华钦,田洁,郑家奎,蒋开锋,罗科.糯稻和非糯稻蜡质基因的新分子标记[J].应用与环境生物学报2006,12(4):461-463
118 Devos K M,Millan T,Gale M D. Comparative RFLP maps of homoeologous group 2 chromoso- mes of wheat and barley[J]. Theoretical and Applied Genetics,1993,85:784-792
119 Li H B,Wang J,Liu A M. Genetic basis of low temperature sensitive sterility in indica japonica hybrids of rice as determined by RFLP analysis[J]. Theoretical and Applied Genetics,1997,95:1092-1097
120方荣,陈学军,缪南生.遗传标记技术及其在辣椒系统发育中的应用研究[J].江西农业学报, 2006,18(2):8-14
121 Bezawada C,Saha S,Jenkins J N. SSR markers associated with root knot nematode resistance genes in cotton[J]. Journal of Cotton Science,2003,7:179-184
122于拴仓.大白菜分子遗传图谱的构建及重要农艺性状的QTL定位[D].中国农业科学院,博士学位论文,2003
123 Guo W Z,ZhangT Z, Pan J J. Identification of RAPD marker linked with fertility-restoring gene of cytoplasmic male sterile lines in upland cotton[J]. Chinese SciBulletin,1998,43:52-54
124 Haanstra J P W,Wye C,Verbakel H. An integrated high density RFLP-AFLP map of tomato based on two Lycopersicon esculetumΧL. pennellii F2 populations[J]. Theoretical and Applied Genetics,1999,99:254-271
125杨玉珍,彭方仁.遗传标记及其在林木研究中的应用[J].生物技术通报,2006,17(5):788-791
126李景欣.内蒙古冰草种质资源遗传多样性研究[D].内蒙古农业大学,博士学位论文,2005
127马缘生,谭富娟,李灵芝,柴建方,范传珠,马榕荫,周红立,周涛.异花授粉作物大白菜和荞麦基因库种子繁殖技术研究[J].中国农业科学,2000,33(2):16-22.
128 Arora R K. Plant exploration and germplasm collection[C].In:Paroda R.S.and Arora R.K.(de.) Plant germplasm Resources Conservation and Management,published by IBPGR,New Delhi,India.1991.
129 Eujayl I,Baum M. A genetic linkage map of lentil(Lens culinaris)[J].Theoretical and Applied Genetics,1998,97:83-89.
130沈金雄,郭庆元,张秀荣,赵应忠,冯祥远,陈和兴,伍晓明.中国芝麻种质资源的聚类分析[J].华中农业大学学报,1995,14(6)532-536.
131樊叶杨,庄杰云,李强.水稻株高QTL分析及其与产量QTL的关系[J].作物学报,2001,27 (6):915-992.
132 Edwards A,Civitello A,Hammond H A,Caskey C T. DNA typing and genetic mapping with trimeric and tetrameric tandem repeats [J].Genet.,1991,97:746-765.
133付福友.甘蓝型油菜遗传图谱的构建和品质相关性状的QTL分析[D].西南大学,博士学位论文,2007
134聂以春,左开井,张献龙.RAPD标记在棉属种间杂种后代检测中的应用[J].中国农业科学,2000,33(5):25-29.
135 Ge X J,Sun M. Reproductive biology and genetic diversity of a cryptoviviparous mangrove Aegiceras corniculatum (Myrsinaceae) us ingallozyme and inter-simple sequence repeat(ISSR)analysis[J]. Molecular Ecology,1999,8:2061-2069.
136 Falconer D S. Introduction to quantitative genetics(2ed.)[M]. Longmn Inc.,New York,1981.
137 Nei M,Li W H. Mathematical model for studying genetic variation in terms of restriction endonucleases[J]. Proc.,Natl.,Acad ,Sci.,USA,1979,76:5269-5273.
138 Nei M. Molecular evolutionary genetics[M].Columbia Univ. Press, New York.1987.
139彭秀玲.基因工程实验技术[M].湖南科学技术出版社,1997
140 PERani J. SYN-TAX-pc-supplement 3:Mscintosh verion[M]. Abstr.Bot,1990,14:23-29.
141 Falconer D.S. Introduction to quantitative genetics(3ed.)[M]. Longman scientific and Technical, New York,USA,1989.
142徐云碧,朱立煌.分子数量遗传学[M].北京:中国农业出版社,1994
143云锦凤,王照兰,杜建才.加拿大披碱草与老芒麦种间杂交及F1代细胞学分析[J].中国草地,1997,(1):32-35.
144 Prabhu R.R,Webb D,Jessen H,Luk S,Smith S,Gresshoff P.M.. Geneticrelatedness among soubean genotypes using DNA amplification fingerprinting,RFLP and Pedigree[J].Crop Sci.,1997,37:1590-1595.
145 Huang J C,Sum M. Genetic diversity and relationship of sweetpotato and its wild relatives in Ipomoea series Batatas (Convolvulaceae) as revealed by inter-simple sequence repeat(ISSR) and restriction analysis of chloroplast DNA[J]. Theoretical and Applied Genetics,2000,100:1050-1060.
146 Liu C J. Geographical distribution of genetic in stylosanthes scabra revealed by RAPD analysis[J]. Euphytica,1997,98:21-27.
147胡志昂.遗传多样性的定义.研究新进展和新概念.生物多样性与人类未来[M].中国林业出版社,北京.1998.
148汪殿蓓,暨淑仪,陈飞鹏.植物群落物种多样性研究综述[J].生态学杂志,2001,20(4):55-60.
149 Dnaiel Z V,Dnaiel P. Patterns of morphological variation and diversity of Coocs nucifera (arecaecae) in Mexico[J]. American Journal of Botany,1998,85(6):855-865.
150罗建勋,顾万春..云杉天然群体种实性状变异研究[J].西北农林科技大学学报,2004,32(8):60-66.
151李斌,顾万春,卢宝明.白皮松天然群体种实性状表型多样性研究[J].生物多样性,2002,10 (2):181-188.
152 Patricia C G,Enriqu E L ,Filogonio M P.. Patterns of morphological variation,diversity,and domestication of wild and cultivated populations of Agave in Yucatan,Mexico[J]. American Journal of Botany ,1996,83(8):1069-1082.
153王小平,刘晶岚,王九龄,刘春江.白皮松种子及球果形态特征的地理变异[J].北京林业大学学报,1998,20(3):25-31.
154孟庆杰,王光全,董绍峰.桃叶片组织解剖结构特征与其抗旱性关系的研究[J].干旱地区农业研究,2004,22(3):123-126.
155王志峰,孙日飞.山东省黄瓜地方黄瓜品种的遗传亲缘关系AFLP研究[J].园艺学报,2004,31(1):103-105.
156杜娟,马小军,李学东.半夏不同种质资源AFLP指纹系谱分析及其应用[J].中国中药杂志,2006,31(1):30-33.
157房经贵,乔玉山,章镇.AFLP在芒果品种鉴定中的应用[J].广西植物,2001,21(3):281-283.
158黄建安,李家贤,黄意欢.茶树品种资源遗传多样性的AFLP研究[J].园艺学报,2006,33(2):317-322.
159杨朝东,王健,张俊卫.梅花不同样本间亲缘关系的AFLP初步分析[J].中国农业科学,2005,38(10):2084-2089.
160石胜友,成明昊,梁国鲁.变叶海棠遗传多样性的AFLP分析[J].园艺学报,2006,33(2):381-384.
161于歆,徐昌德.双丰系列甜菜品种AFLP亲缘关系及系谱分析分子植物育种[J].分子植物育种,2004,12(2):229-234.
162于卓,马艳红,李小雷,周亚星,李晓宇,李造哲.加拿大披碱草与披碱草、圆柱披碱草2个种间杂种F1及其亲本的AFLP分析[J].草地学报,2008,16(5):431-435.
163钱韦,葛颂,洪德元等.采用RAPD和ISSR标记探讨中国庆粒野生稻的遗传多样性[J].植物学报,2000,42(7):741-750.
164唐启义,冯明光.实用统计分析及其DPS数据处理系统[M].北京:科学出版社,2002.
165李鸣,谭裕模,李杨瑞,李容柏,高国庆.甘蔗(Saccharum officinarum L.)品种遗传差异的AFLP分子标记分析[J].作物学报,2004,30(10):1008-1013
166翟文强,田清震,贾继增,董玉琛.哈密瓜杂交种纯度的AFLP指纹鉴定[J].园艺学报2002, 29 (6): 586-587
167田雷,曹鸣庆,王辉,郭晶心,周乃元,孙江,贾希海. AFLP标记技术在鉴定甘蓝种子真实性及品种纯度中的应用[J].生物技术通报,2001,(3):38-40,44.
168 Zietkiewicz E , Rafalski A , Labuda D. Genome fingerprinting by simple sequence repeat(ISSR)-anchoredpolymerarase chain reaction amplification[J]. Genomics,1994,20:176-183
169 KojimaT,NagalkaT,Noda K. Genetic linkage map of ISSR and RAPD markers in Einkorn Wheat in relation to that of RFLP markers[J]. Theoretical and Applied Genetics,1998,96:37-45.
170赵茹,程舟,陆伟峰,卢宝荣.基于分子标记的野生大豆居群遗传多样性估算与取样策略[J].科学通讯,2006,51(9):1042-1048
171黄文霞,何觉民,朱宏波.蓖麻种质资源遗传多样性的ISSR分析[J].西北农业学报,2008 ,17(1):182-184,187
172褚云霞,汤庚国,张永春,靖相密.蜡梅ISSR反应体系的优化[J].北方园艺,2007,(10):161-163
173罗淑萍,曾斌,孙晋科,李疆.野扁桃ISSR -PCR反应体系的优化[J].经济林研究2007, 25(3):1-5
174周延清,景建洲,李振勇,浩健,贾敬芬.怀地黄ISSR扩增条件优化的研究[J].西北植物学报2004,24(1):6-11
175沈永宝,施季森,赵洪亮.利用ISSR DNA标记鉴定主要银杏栽培品种.林业科学,2005,41(1):202-204
176李国珍.染色体及其研究方法[M].北京:科学出版社,1985:303-314.
177李懋学,张赞平.作物染色体及其研究技术[M].北京:中国农业出版社,1996:307-311.
178李贵全.细胞学研究基础[M].北京:中国林业出版社,2001:6-8.
179黎中明,林文君.细胞遗传学[M].成都:四川大学出版社,1988:44-49.
180于卓,云锦凤,李造哲.加拿大披碱草与野大麦及其属间杂种细胞遗传学研究[A].见:中国国际草业发展大会暨中国草原学会第六届代表大会学术及产业研讨会论文集[C].北京:中国农业出版社,2002.
181苏东梅.柿属君迁子和湖南九个乡土品种过氧化物酶同工酶的研究[J],武汉植物学研究,1991,9(3):253-258.
182梁宏伟,刘姝,陈发菊,熊丹.银鹊树种子萌发过程中的过氧化物酶和酯酶同工酶的变化[J].种子,2006,25(5):38-40.
183葛颂.同工酶和植物进化生物学研究.植物进化生物学[M].武汉:武汉大学出版社1994,153- 208.
184张学科,毛子军,宋红,孟斌.五种落叶松遗传关系的等位酶分析[J].植物研究,2002,22(2):224-230.
185蓝登明,何文兴. 8个沙棘品系间等位酶水平遗传多样性研究[J].沙棘,2003,16(4):11-15.
186全妙华,陈东明,付明,姚元枝.四棱豆过氧化物酶同工酶的研究[J].中国农学通报,2006,22(4):203-206
187马云峰,薛瑰玲,尚富德,大白菜幼苗过氧化物同工酶的研究[J].河南大学学报,2006,3,23(1):82-84.
188沙伟,孟凡玲,冯昌军. 10个苜蓿品种过氧化物同工酶POD分析[J].北方园艺,2007,(1):150-152.
189陈燕珍,胡丽莲,陶毅明,梁杨琳.不同生育期菊芋叶片过氧化物同工酶表达特点的研究[J].生物学杂志,2007,2(24):25-46.
190 Joseph,L.S.and Boouwkamp,J.C..Karyomorphology of several species of Phaseolusand Vigna[J].Cytologia,1978,43:595-600.
191解新明,云锦凤,赵冰,卢小良,李秉滔.蒙古冰草遗传多样性的等位酶分析[J].草业科学, 2001,18(6):6-11.
192闫忠业,伊凯,李作轩,刘志,王冬梅,杨峰.富士系苹果过氧化物同工酶研究[J].沈阳农业大学学报,2006,37(4):578-581.
193陈艳秋,李玉梅.黑木耳菌株氧化物同工酶的研究[J].吉林农业大学学报,2005, 27(1): 26-28.
194王中仁.植物等位酶分析[M].科学出版社,北京,1996.
195孔祥祯,曲延英,丁勇,何宗祥,楚金平.新疆罗布麻属和白麻属植物的染色体数目[J].八一农学院学报,1993,16(4):38-39.
196陈彦云,曹君迈,李国旗,任辉丽,谢敏.罗布麻染色体核型分析[J].北方园艺,2008,(1):200-202
197张龙翔,张庭芳,李令媛.生化试验方法和技术[M].高等教育出版社,1981,11:119-123.
198张相歧.番茄属四个钟的过氧化物酶同工酶的分析[J].植物研究,1987,7(4):131-136.