摘要
目的:探索制作慢性炎症所致不孕大鼠模型,探讨中药通络助孕方对慢性炎性不孕大鼠输卵管病理形态学、细胞因子IL-2、IL-6和受孕率的影响及其疗效机理。
方法:用“混合菌接种法”制作大鼠慢性输卵管炎性不孕动物模型,以不同剂量的通络助孕方和对照药妇炎康对模型大鼠治疗后,观察光镜和电镜下输卵管病理形态学改变情况,用ELISA法检测血清和输卵管组织中IL-2、IL-6含量,并观察大鼠受孕情况。
结果:模型大鼠输卵管组织呈慢性炎症表现,并且导致不孕发生。通络助孕方和对照药能不同程度恢复模型大鼠输卵管病理形态学改变,降低血清和局部输卵管组织中IL-2、IL-6水平,提高受孕率。
结论:“混合菌接种法”可以成功制作大鼠炎性不孕症模型;炎性不孕不一定完全由输卵管阻塞引起,细胞因子IL-2、IL-6的失衡与慢性炎性不孕症的发生发展有关;通络助孕方能改善炎性不孕大鼠输卵管结构和功能,对血清及输卵管组织中IL-2、IL-6含量有调节作用,从而对大鼠炎性不孕具有很好的治疗作用。
Purpose: To explore and make big rats modeI of sterility
caused by chronic salpingitis, observe the effect of
Tongluozhuyunfang (TLF in brief ) on the patho1ogy and
morphallaxis of oviduct, interleukin IL-2 \ IL-6 in serum and
oviduct, and trace the rats pregnant rate for exploring its curative
mechani sm.
Methods: Mixed bacterium was used for making salpingitis
caused sterility rats model, treated by different doses of TLF
contrasted with Fuyankang (FYK ), the pathology and morpha1laxis
of oviduct with Light and Transmission electron microscope was
observed, the content of IL-2. IL-6 in serum and oviduct was
checked by ELISA method, pregnant results also was traced.
Results: The fallopian tube of model rats was fOund with chronic
salpingitis, and result in sterility. TLF and FYK can resume the
pathology and morphallaxis of oviduct in varying degrees, reduce
IL-2. IL-6 both in serum and oviduct, and so raise the pregnant
rate.
Conclusion: Big rats model of sterility caused by chronic
salpingitis using mixed bacterium can be successfully made; the
sterility is not caused by tubal obstruct completely, unbalance of
IL-2. IL-6 has relationship with this kind of sterility; TLF can
improve the tube structure and function, accommodate IL-2. IL-6
both in serum and oviduct, and so has better therapy on the
sterility.
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