长叶地榆的化学成分研究
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摘要
长叶地榆(Sanguisorba officinalis L var longifolia(Bert)Yu et Li)是蔷薇科(Rosaceae)地榆属(Sanguisorba L)植物中的一个种,主要分布于中国东北、华北、华东、广西及贵州等地,具有凉血止血,解毒敛疮之功效。本论文对长叶地榆化学成分进行了系统的研究。
将长叶地榆的根茎的乙醇提取物依次用氯仿、乙酸乙酯、正丁醇萃取,应用色谱法分离氯仿部分和乙酸乙酯部分化合物,借助波谱技术(GC-MS、~1H-NMR、~(13)C-NMR)鉴定化合物的结构。
从氯仿部分、乙酸乙酯部分中共分离到13个化合物,目前完全鉴定了其中9个化合物的结构,分别为:大黄酚(Ⅰ)、大黄素甲醚(Ⅱ)、β-谷甾醇(Ⅲ)、阿魏酸(Ⅳ)、熊果酸(Ⅴ)、没食子酸(Ⅵ)、槲皮素(Ⅶ)、山奈酚(Ⅷ)、儿茶素(Ⅸ)。所有化合物均为首次从该植物中分离得到,并测定该植物中的有效成分的含量。总黄酮含量3.51%,而鞣质含量为9.60%。
Sanguisorba officinalis L var longifolia(Bert)Yu et Li belongs to the species of Rosaceae in Sanguisorba L and is mainly distributed in northeast,north china,east china,guangxi province and guizhou province etc.This plant can be used to cool blood, stop bleeding and detoxify.This thesis made a systemic study on the chemical constituents of Sanguisorba officinalis L var longifolia(Bert)Yu et Li.
The EtOH extracts of Sanguisorba officinalis L var longifolia(Bert)Yu et Li was extracted by CHCl_3,EtOAc and n-BuOH by turns.Using chromatographic methods to isolate compounds from Sanguisorba officinalis L var longifolia(Bert)Yu et Li in the CHCl_3 layer and EtOAc layer and elucidate their structures by spectral methods(GC-MS、~1H-NMR、~(13)C-NMR).
There were 13 compounds purified from the chloroform layer and EtOAc layer. The 9 compounds had been identified at present,and they are chrysophanol(Ⅰ), physcion(Ⅱ),β-sitosterol(Ⅲ),ferulic acid(Ⅳ),Ursolic acid(Ⅴ),gallic acid (Ⅵ),quercetin(Ⅶ),kaempferol(Ⅷ),catechin(Ⅸ).All compounds were isolated from Sanguisorba officinalis L var longifolia(Bert)Yu et Li for the first time.Effective constituents of Sanguisorba officinalis Lvar longifolia(Bert)Yu et Li in the root were determined.The content of total flavonoids was 3.51%and the content of tannin was 9.60%.
将长叶地榆的根茎的乙醇提取物依次用氯仿、乙酸乙酯、正丁醇萃取,应用色谱法分离氯仿部分和乙酸乙酯部分化合物,借助波谱技术(GC-MS、~1H-NMR、~(13)C-NMR)鉴定化合物的结构。
从氯仿部分、乙酸乙酯部分中共分离到13个化合物,目前完全鉴定了其中9个化合物的结构,分别为:大黄酚(Ⅰ)、大黄素甲醚(Ⅱ)、β-谷甾醇(Ⅲ)、阿魏酸(Ⅳ)、熊果酸(Ⅴ)、没食子酸(Ⅵ)、槲皮素(Ⅶ)、山奈酚(Ⅷ)、儿茶素(Ⅸ)。所有化合物均为首次从该植物中分离得到,并测定该植物中的有效成分的含量。总黄酮含量3.51%,而鞣质含量为9.60%。
Sanguisorba officinalis L var longifolia(Bert)Yu et Li belongs to the species of Rosaceae in Sanguisorba L and is mainly distributed in northeast,north china,east china,guangxi province and guizhou province etc.This plant can be used to cool blood, stop bleeding and detoxify.This thesis made a systemic study on the chemical constituents of Sanguisorba officinalis L var longifolia(Bert)Yu et Li.
The EtOH extracts of Sanguisorba officinalis L var longifolia(Bert)Yu et Li was extracted by CHCl_3,EtOAc and n-BuOH by turns.Using chromatographic methods to isolate compounds from Sanguisorba officinalis L var longifolia(Bert)Yu et Li in the CHCl_3 layer and EtOAc layer and elucidate their structures by spectral methods(GC-MS、~1H-NMR、~(13)C-NMR).
There were 13 compounds purified from the chloroform layer and EtOAc layer. The 9 compounds had been identified at present,and they are chrysophanol(Ⅰ), physcion(Ⅱ),β-sitosterol(Ⅲ),ferulic acid(Ⅳ),Ursolic acid(Ⅴ),gallic acid (Ⅵ),quercetin(Ⅶ),kaempferol(Ⅷ),catechin(Ⅸ).All compounds were isolated from Sanguisorba officinalis L var longifolia(Bert)Yu et Li for the first time.Effective constituents of Sanguisorba officinalis Lvar longifolia(Bert)Yu et Li in the root were determined.The content of total flavonoids was 3.51%and the content of tannin was 9.60%.
引文
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38.曾万玲,宋杰云,岑燕飞.地愉水煎液抗实验性腹泻及其他药理作用的研究.贵阳医学院学报,1992,14(4):55-57.
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43.D.L.Cheng,X.P.Cao.Pomolic acid derivatives from the root of Sanguisorba officinalis.Phytochemistry,1992,31(4),1317-1320.
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45.Y.Mimaki,M.Fukushima,A.Yokosuka,Y.Sashida,S.Furuya,H.Sakagamilsolation and characterization of six metyl glucoside gallates and a gallic acid glueoside gallate from Sanguisorba officinalisL.Chem.Pharm.Bull.1984,32(1),117-121.
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2.刁英智,任向宏,谷亚明.“溃疡粉”治疗溃疡病20例临床分析.哈尔滨中医,1965,9:24-27.
3.于海,黄泰康,吴春福.中药现代化发展的进程和趋势.中草药,2005,36(1)147-149.
4.中国医学科学院药物研究所编.中草药有效成分的研究.北京人民卫生出版社,1972,351-352.
5.王方宇.蔷薇红景天化学成分研究.沈阳药科大学,硕士学位论文:47.
6.王立涛,崔胜杰,靳宪辉.地榆酒治崩漏症.浙江中医杂志,1965,3:4.
7.王银朝.滇山茶和幌伞枫化学成分研究及变异黄芪中苦马豆素的结构再鉴定[D].西北农林科技大学,学位论文:28.
8.王溶生.中药药理与应用.人民卫生出版社,1983,406.
9.王满力,陈桐,王明力.地榆皂试和鞣质在柱分离中的紫外光谱研究.贵州工业学院学报,1994,8:332.
10.冉懋雄.推进中药现代化建设加快贵州中药产业的发展.贵州农业科学,2004,32(1):73-75.
11.包贝华,张丽,丁安伟.分光光度法测定荆芥炭中总黄酮的含量.时珍国医国药,2004,15(5):264-265.
12.刘小柔,王存芳,严启新.银杏叶提取物在保健食品总黄酮测定方法的应用[J]分析检测,2005,6:174-178.
13.刘真秀,林立文,王丹丹.地榆,明矾,磺胺混合糊剂治疗宫颈糜烂的初步观察.浙江学报,1959,6:550-552.
14.孙盛,孟燕彬.地愉提取物对紫外线B导致大鼠皮肤光损伤的抑制作用国外医学中医中药分册,2002,24(5):299-300.
15.吴开云.冰片,虎杖,地榆抑菌作用的实验研究.江西医学院学报,1996,36(2):53-55.
16.张帆,付铁军,刘忠荣,彭树林,丁立生.中药地榆的化学成分分析.分析试验 室,2003,22(增刊):15-17.
17.张妍,李厚伟,张永春.山楂中总黄酮几种提取分离方法的考察及含量测定.哈尔滨医科大学学报,2001,35(3):183-184.
18.李鸿勇,吕情花.地榆治疗溃疡大出血60例临床观察.中华内科杂志,1960,3:248.
19.杨九艳,敖强,高敏,杨明博.中蒙药锦鸡儿鞣质含量的测定.中国民族医药杂志,2005,2:26.
20.苏妙贤.小紫金牛和猴耳环的化学成分和生物活性研究.暨南大学,硕士学位论文:45.
21.邹建国,荣阳,张闯.地榆黄莲粉治疗烧伤12例分析.湖南医学院学报,1958,2:71.
22.周艳,赵鑫.地榆防治狂犬病的探讨.江苏中医,1959,8:38-87.
23.林静,金养麦的生药学及化学成份研究.湖北中学院,硕士学位论文:20.
24.竺叶青,施大文,李自力.中药地榆的薄层层析鉴别及鞣质含量测定.上海医科大学学报,1995,22(2):149-150.
25.竺叶青,施大文,李自力.中药地榆的药源调查及商品鉴定.上海医科大学学报,1995,22(1):62-64.
26.郑颖,蓝闽波,徐德生.聚酞胺树脂分离苷草总酮及抗氧化活性研究.中成药.2006,28(10):1521-1522.
27.临床观察.中医药信息杂志,2004,11(1):70-71.
28.姜云梅,杨五禧,吴立军,李维贤.中药地榆化学成分的研究.西北药学杂志,1993,8(1):17-19.
29.姜贵全.地榆中药用活性成分提取工艺及稳定性研究[D].东北林业大学,学位论文:14.
30.赵德芬,井光宗.地榆三黄粉外敷治疗褥疮.中国民间疗法,2003,11(5):23.
31.徐少平,杨鸿,刘小会.加味地榆散的药效学研究.陕西中医学院学报,2001,24(2):34-35.
32.秦国伟,陈梅玉,徐任生.地榆化学成分的研究.中草药,1991,22(11):483-485.
33.袁昌衡,周启贵,杨飞等.80种中药水煎液对淋球菌的抑制试验.中国医院药学杂志,1997,17(10):508-509.
34.贾天柱,胡凤艳,李军,解士全,韩久利.狗脊不同炮制品中鞣质的含量测定.辽宁中医杂志,2001,28(5):305.
35.梁燕飞,农金芳.复方地榆汤治疗肺出血.江苏中医,1964,2:封三.
36.黄雪芳,彭宣宪,吴开云.虎杖,地榆联合抗菌作用的实验研究.江西医学院学报,1997,37(2):1-2.
37.黄雪芳,彭宣宪,傅文红.甘草或冰片与虎杖和地榆配伍对其抑菌能力的影响.江西科学,1998,16(2):92-96.
38.曾万玲,宋杰云,岑燕飞.地愉水煎液抗实验性腹泻及其他药理作用的研究.贵阳医学院学报,1992,14(4):55-57.
39.程东亮,曹小平,邹佩秀,杨萍.中药地榆黄酮等成分的分离与鉴定.中草药,1995,26(11):570-571.
40.蔡炼.地榆治疗烧伤作用研究的初步报告.四川医学院学报,1959,2:98-104.
41.谭静玲.要用大黄抗病毒活性成分研究.北中医学大学,硕士学位论文:35.
42.A.Renbo,Y.H.Tian,O.Hyuncheol et.al.In vitro free radical scavenging and hepatoprotetive compound from Sanguisorbae Radix[J].Natural Product Science,Korean Society of pharmacognosy,Korea epublic,2005,11(3),119-122.
43.D.L.Cheng,X.P.Cao.Pomolic acid derivatives from the root of Sanguisorba officinalis.Phytochemistry,1992,31(4),1317-1320.
44.G.I.Nonaka,K.Ishimaru,I.N.Tanaka,Tannins and related compounds,ⅩⅦ.Galloylhamameloses from Castanea crenata L and Sanguisorba officinalisL.Chem Pharm Bull,1984,32(2),483-489.
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