摘要
本研究系统探讨了猪对陈化早籼糙米的养分利用机理。(1)以当年收获及贮存三年的早籼稻谷脱壳后所获早籼糙米为材料,采用单螺杆挤压膨化机处理陈化糙米。设水解温度37℃,pH值7.0,水解4 h,研究不同外源α-淀粉酶和糖化酶对早籼糙米淀粉的体外水解效果。结果表明:综合考虑添加成本和水解效果,针对早籼糙米的外源酶组合为α-淀粉酶2500
U/Kg,糖化酶20000 U/Kg日粮。(2) 考察螺杆转速、套筒温度、喂料速度、原料水分以及添加外源酶对陈化早籼糙米挤压膨化效果的影响。结果表明:提高螺杆转速或喂料速度降低陈化早籼糙米的淀粉糊化度和挤压膨胀度;而提高套筒温度、保持适当原料水分和加入足量高温淀粉酶可提高挤压膨化效果。(3)研究不同贮存期及膨化早籼糙米添加外源酶对断奶仔猪小肠粘膜二糖酶、血液生化指标及生产性能影响。两个试验均采用2×2因子设计,分别以不同贮存期(当年收获、贮存三年)和添加外源酶(添加、不添加)(试验三)和膨化(膨化、不膨化)及添加外源酶(添加、不添加)(试验六)为考察因子。各选取26±2日龄断奶的杜洛克×长白×大白断奶仔猪96头(公母各半),在第2、4周末每个处理各选取6头健康去势公猪,屠宰并收集十二指肠、空肠(前、中、后段)和同肠各段粘膜并检测二糖酶、淀粉酶和糖化酶活性,分析血清尿素氮和葡萄糖含量,考察猪只各阶段生产性能。结果表明:当年收早籼糙米显著提高十二指肠和空肠麦芽糖酶、海藻糖酶、异麦芽糖酶和蔗糖酶的活性(P<0.05);膨化显著提高十二指肠粘膜麦芽糖酶活性,但显著降低空肠麦芽糖酶、异麦芽糖酶、蔗糖酶和乳糖酶活性(P<0.05)。添加外源酶显著提高第2周猪只十二指肠麦芽糖酶、异麦芽糖酶和蔗糖酶活性(P<0.05),显著提高第4周猪只空肠麦芽糖酶、异麦芽糖、海藻糖酶和乳糖酶以及回肠海藻糖酶活性(P<0.05)。新旧糙米、膨化及添加外源酶对血清葡萄糖和尿素氮无显著影响,断奶仔猪对新糙米的利用率显著优于陈糙米(P<0.05),膨化处理陈化早籼糙米显著降低饲料效率(P<0.05)。(4)研究不同贮存期及膨化早籼糙米添加外源酶对仔猪表观养分消化率、食糜酶活性以及还原糖含量的影响。各选用6头健康去势公猪,回肠末端安装T型瘘管,按6×6拉丁方设计分别饲喂单—新鲜或陈化早籼糙米,并在此基础上添加不同外源酶组合(无锡酶、广东酶及不添加)共六种试验日粮(试验四)以及高低糊化度和未处理陈化早籼糙米,并在此基础上添加或不添加外源酶(无锡产α-淀粉酶2500 U/kg+糖化酶20000 U/kg日粮)共计六种试验日粮(试验七),研究不同贮存期及膨化早籼糙米添加外源酶对猪表观养分消化率、食糜碳水化合物消化酶活性和还原糖的影响。结果表明:新糙米日粮猪蛋氨酸、异亮氨酸和缬氨酸的回肠表观消化率优于陈糙米日粮试猪(P<0.05),适度膨化有提高淀粉表观回肠消化率的趋势(P=0.075)。添加外源酶提高蛋氨酸、赖氨酸、苏氨酸及异亮氨酸的表观回肠消化率(P<0.05),不同贮存期及膨化早籼糙米添加外源酶对粪表观氨基酸消化率无显著影响(P>0.05)。陈化糙米降低猪回肠末端食糜中碳水化合物消化酶的活性,但添加外源酶提高回肠食糜淀粉酶(P<0.05)和糖化酶活性(P<0.01)。不同贮存期及膨化早籼糙米添加外源酶对食糜和粪中还原糖含量无显著影响(P>0.05)。
Seven relative trails were conducted to investigate the mechanism of nutrients availability for storage early, long-grain non-glutinous rice (ELGNR, indica rice) in swine diets. The nutrients of different storage time and extrusion degree brown rice were analyzed firstly, then an in vitro trail was conducted to investigate the effect of exogenous enzyme on starch hydrolization degree for fresh or storage brown rice, the results suggested that the appropriate activity of exogenous enzyme for brown rice was a-amylase 2500 U/Kg diet and glucoamylase 20000 U/Kg diet. A continuative process trail was conducted to investigate the extrusion process parameters on starch gelatinization degree, the results showed that the starch gelatinization degree increased with the increasing of the extrusion temperature, maintaining appropriate ingredient moisture and supplementing with a-amylase. Two growth trails in a 2 x 2 factorial design used 192 weaned pigs (96 pigs per trail) were then conducted to investigate the effects of different storage time and extruded brown rice supplemented with exogenous enzyme on intestinal disaccharidase activity, serum glucose and urea nitrogen concentration and growth performance. The factors included brown rice source (fresh or stored) and enzyme supplementation (with supplementation or without supplementation) (trail 3) and extrusion status (extruded or non-treated) and enzyme supplementation (with supplementation or without supplementation) (trail 6), respectively. The results of trail 3 showed that pigs fed fresh brown rice had higher duodenal and jejunal maltase, isomaltase, sucrase and trehalase activity (P < 0.05), extrusion (P = 0.042) and enzyme (P - 0.003) supplementation resulted in a higher maltase activity for weaned pigs in duodenum at d 14. The results of trail 6 showed that extrusion decreased the activity of maltase (P = 0.016), isomaltase (P = 0.025), sucrase (P = 0.028), and lactase (P = 0.002) for weaned pigs in jejunum at d 28. Enzyme supplementation increased the activity of maltase (P = 0.016), isomaltase (P = 0.025), sucrase (P < 0.05) for weaned pigs at d 14, and enzyme supplementation increased activities of duodenal maltase (P = 0.004), isomaltase (P = 0.039), trehalase (P = 0.039), and lactase (P = 0.009) for the weaned pigs in jejunum at d 28. Weaned pigs fed the extruded or stored CSBR diet had a worse feed/gain (F/G) (P < 0.05). In order to investigate the effects source or extruded brown rice supplemented with exogenous enzyme on apparent ileal and fecal digestibility of nutrients, the carbohydrase activity and deoxidized glucose concentration in digesta, two digestion trails used 12 male pigs fitted with a simple "T" cannula at the terminal ileum were used and conducted in a 6 x 6 Latin Square design. The pigs in trail 4 were fed fresh or stored brown rice with or without two exogenous enzyme combinations (625 or 2500 mg/kg a-amylase and 200 or 1600 mg/kg glucoamylase) and one control (without enzyme supplementation). Pigs in trail 7 were fed non-treated or light or normal temperature extruded SBR without or with 625 mg/kg a-amylase and 200mg/kg glucoamylase supplementation. Chromic oxide was incorporated into the diets as an indigestible marker. The present results suggested that weaned pigs fed fresh brown rice had higher apparent ileal digestibility (AID) of methionine, isoleucine and valine compared to
pigs fed storage brown rice (P < 0.05), extrusion tended to improve the apparent ileal digestibility (AID) of starch (P = 0.075), but the AID of energy, DM, CP and AA for grower pigs were not influenced by extrusion (P > 0.05). Enzyme supplementation increased the AID of lysine, methionine, threonine and isoleucine (P < 0.05). Normal temperature extrusion decreased the activity of carbohydrase in digesta, however, enzyme supplementation increased the activity of α-amylase (P < 0.05) and glucoamylase (P < 0.01) in digesta.
引文
包劲松,徐建平,包志毅,傅俊杰.2002.淀粉特性与工业应用研究进展.浙江大学学报(农业与生命科学版),28(6):694-702.
卞科,路茜玉.1992.大米陈化过程中二硫键变化与蒸煮大米质构特性的关系.中国粮油学报,4:38-41.
陈印军,尹昌斌.1999.对南方双季稻主产区“玉米替代”的反思.中国农村经济,2:20-25.
程美玲,高士争,张曦,戴志明.2001.膨胀加工对饲料营养成分的影响.饲料工业,22(4):7-9.
程志斌,葛长荣.2002.粘膜二糖酶.饲料研究,(3):15-18.
戴文波特.1976.消化道生理学.(北京医学院生理教研组译).北京:科学出版社,pp 269-281.
戴有盛.1994.食品生化与营养.(第一版).北京:科学出版社,pp 18-21.
甘智林.1986.细胞壁与稻米陈化.郑州粮食学院学报,4:70-73.
龚朝辉.1999.用稻谷代替玉米喂猪的效益试验.江西畜牧兽医杂志,2:42.
何瑞国,麻益良,王艳青,赵景杨,王怀祥.1994.猪消化代谢试验对糙米营养价值的研究.华中农业大学学报,113(3):268-273.
何瑞国,王玉莲,马立保,和希顺,郑桂菊,胡洪涛,王志文.1999.早朵籼稻糙米代替玉米日粮对肉鸡增重效果的研究.华中农业大学学报,18(2):166-168.
何瑞国,王玉莲,马立保,黎明,张顺喜,林发光,胡建国.2000.湖北早籼糙米营养价值研究(Ⅱ):糙米代替玉米作饲料对肥猪增重和肉品质影响的研究.中国粮油学报,15(1):50-52.
贺建华,黄美华,金宏,曾淑元,徐庆国.2000.饲料用稻谷和糙米的营养特性.见:韩友文,主编.中国畜牧兽医学会动物营养学分会第六届全国会员代表大会暨第八届学术研讨会论文集.哈尔滨:黑龙江人民出版社,pp 189-193.
洪平.1996.饲料原料要览.北京:海洋出版社,pp 12-18.
计成,周庆,田河山.1997.断奶前(胰、小肠内容物)几种消化酶活性变化的研究.动物营养学报,9(3):7-12.
季清娥,徐珍秀,兰盛银.1997.糙米氨基酸含量与其壁层厚度的关系初探.湖北农学院学报,17(2):159-160.
蒋中柱.2000.糙米保鲜理论和储藏技术.粮食科技与经济,4:12-14.
金征宇.2000.挤压膨化与后添加技术在饲料工业中的应用.美国大豆协会:饲料技术讲座文集(2000-2001).pp 139-171.
阑万煌,萧浪涛,彭克勤,洪亚辉,邹冬生.2001.稻米垩自的形成及调控.湖南农业大学学报(自然科学版),27(3):234-239.
李俊波,刘大建,舒建成,曹章元,龙向阳.2001.膨化日粮对断奶仔猪生产性能影响.饲料工业,(7):37-38.
李俊波,舒建成,刘大建,曹章元,罗华军,郭吉元.2000.霉菌吸附剂对肉仔鸡生产性能影响.饲料工业,(6):40-41.
李丽立,张彬,杨坤明,黄瑞林.2002.稻谷替代玉米饲喂肉鸭对资源和环境的影响.长江流域资源与环境,11(1):60-63.
梁海平,陈匡辉,陆伟,谢国强,王博,何余涌.1999.全早稻型猪用浓缩料饲喂生长肉猪中试报告.养猪,2:18-20.
刘桂才.1999.我国1999年上半年大米市场分析及下半年展望.中国稻米,4:30.
刘强.我国麦类饲料中非淀粉多糖抗营养作用机理的研究:[博士学位论文].北京:中国农业科学院,1999.
吕雪娟,杨祟,杨军,何秀英.1999.贮藏对籼稻米蛋白质营养品质的影响.华南农业大学学报,20(1):63-67.
梅懋华.1990.消化道生理学与临床.北京:人民卫生出版社,pp 220-228.
闵琛,许春娣,陈舜年.2003.双糖酶缺乏症.国外医学儿科学分册,30(4):178-180.
闵瑞林.2002.早籼稻产业形势及市场走势分析.江西粮油土肥信息网(http://www.jx-agri.gov.cn/lytf).
宁正祥.1998.食品成分分析手册.北京:中国轻工业出版社,pp 680-688.
钱海峰,陈玉铭.2001a.大米陈化过程中淀粉性质变化研究.粮食与饲料工业,11:1-3.
钱海峰,姚惠源.2001b.大米陈化过程中的组织学变化研究.粮食储藏,29(1):41-45.
任顺成,周瑞芳.2001.大米陈化过程中蛋白质与大米质构特性的变化.郑州工程学院学报,22(1):43-45.
任顺成,周瑞芳,李永红.2002.大米陈化过程中谷蛋白与大米质构特性的变化.中国粮油学报,17(3):42-46.
沈水宝.外源酶对仔猪消化系统发育及内源酶活性的影响.[博士学位论文].广州:华南农业大学,2002.
沈同,王镜岩.1990.生物化学.北京:高等教育出版社,pp 10-38.
孙哲,王儆.2001.二糖酶及其在动物消化吸收中的作用.动物营养学报,13(3):1-7.
汤玮如,任鹏,黄美华,田科雄,王继成,苗泽荣,张子仪.1992.国产稻谷、糙米的生产概况及饲料营养价值评定.见:张子仪,主编.中国畜牧兽医学会动物营养学分会第六届年会暨学术讨论会中国动物营养研究进展.天津:天津科技翻译出版公司,pp 12-18.
王根庆.1999.不同稻类品种蛋白质和氨基酸含量差异的研究.华北农业学报,6(2):7-12.
王九峰.日粮纤维和淀粉对猪胃肠道微生物区系、能量代谢及离体发酵的影响.[博士学位论文].北京:中国农业大学,2000.
王敏奇,许梓荣,孙建义.2003.早籼稻饲粮中添加酶制剂对猪生长和消化的影响.中国水稻科学,17(2):179-183.
王石瑛.2000.挤压膨化技术及其应用.西部粮油科技,25(3):31-33.
王竹,门建华,杨月欣,洪洁.2002a.抗性淀粉对大鼠锌营养状况的影响.营养学报,24(2):167-170.
王竹,杨月欣,韩军花,门建华,洪洁.2002b.抗性淀粉对饮食诱发葡萄糖耐量异常的预防.营养学报,24(1):48-52.
奚刚,许梓荣,钱利纯.1999.添加外源性酶对猪、鸡内源消化酶活性的影响.中国兽医学报,19:286-289.
夏枚生.2000.早籼稻饲料中添加NSP酶对生长猪生长与消化性能的影响.浙江大学学报(农业与生命科学版),26(5):559-562.
向德标,汪汉华,何瑞国.2001a.早籼稻谷替代玉米的肉牛饲粮粗脂肪在瘤胃中降解特性的研究.粮食与饲料工业,11:21-22.
向德标,汪汉华,夏宏宇.2001b.早籼稻谷替代玉米的肉牛饲粮在瘤胃中干物质与粗蛋白降解规律的研究.粮食与饲料工业,3:27-29.
向涛.1986.家畜生理学原理.北京:农业出版社,pp 267-269.
向远鸿,唐启源,黄燕湘.1990.稻米品质相关性研究Ⅰ:籼型粘稻食味与其它米质性状的关系.湖南农学院学报,16(4):325-330.
谢富弘.1999.挤压饲料、食品的物理-化学变化.美国大豆协会:饲料技术讲座文集(1998-1999).pp 190-214.
谢章学,廖文远,蒋义明,鲁本龙.2002a.用稻谷(糙米)、小麦代替玉米对断奶仔猪增重效果的试验.湖北畜牧兽医,1:9-11.
谢章学,陶云荷,蒋义明,廖文远.2002b.用糙米、小麦代替玉米饲喂瘦肉猪的试验.湖北畜牧兽医, 6:8-10.
熊善柏,赵思明,姚霓,黄红霞.2001.稻米淀粉糊化进程研究.粮食与饲料工业,5:14-16.
徐庆国,贺建华,黄丰,曾淑元,唐启源.2000.早籼稻饲料营养价值的品种间差异研究.湖南农业大学学报,26(1):20-23.
徐学明,谢正军,金征宇.1999.膨胀器与膨胀饲料.中国饲料,(17):25-27.
许梓荣,李卫芬,孙建义.2002.猪胃肠道粘膜二糖酶的性质.动物学报,48(2):202-207.
薛永丰,黄少文,胡奇伟,钱柏洲,武家水.1994.用稻谷饲喂商品瘦肉型猪的研究.中国畜牧杂志,30(6):34-35.
杨参,阚建全,陈宗道,王光慈.2003.抗性淀粉及其生理功能研究新进展.粮食科技与经济,3:41-42.
杨全明.猪消化道酶和组织器官生长发育规律的研究.[博士学位论文].北京:中国农业大学,1999.
杨胜.1993.饲料分析和质量检测技术.北京:北京农业大学出版社,pp 30-31.
杨月欣,R.Vonk,F Stellaard.1999.三种类型玉米淀粉在小肠中消化吸收的研究.营养学报,21(3):284-287
衣杰荣,姚惠源.2001a.淀粉分子结构对形成抗性淀粉的影响.粮食与饲料工业,6:33-35.
衣杰荣,姚惠源.2001b.温度对抗性淀粉形成的影响.粮食与饲料工业,8:37-38.
于炎湖.1992.饲料毒物学附毒物分析.北京:农业出版社,pp122-146.
张德福.早籼糙米在猪日粮中的营养价值评定.[硕士学位论文].北京:中国农业大学,2002.
张宏福,顾宪红.2001.仔猪营养生理与饲料配制技术研究.北京:中国农业科技出版社,pp 15-19.
张宏福,李长忠,顾宪红.1998.断奶日龄对仔猪肠道中乳糖酶活性的影响.农业部动物营养学重点开放实验室动物代谢研究.pp 87-93.
张石蕊,田科雄,王继成,赵玉蓉.1999a.糙米与玉米型饲粮对肉猪饲用价值比较研究.饲料工业,20(5):26-27.
张石蕊,田科雄,王继成,赵玉蓉.1999b.饲用糙米、玉米对猪消化代谢性能的评价.饲料工业,20(8): 31-32.
张石蕊,田科雄,王继成,盛克松,陈仕平.2000.饲用糙米型饲粮对猪鸡肉蛋品质的影响.湖南农业大学学报(自然科学版),26(5):375-378.
张树政主编.1984.酶制剂工业(下).北京:科学出版社,pp 456-512.
张玉生.1994.动物生理学.北京:中国科学技术出版社,pp 267-267.
赵思明,熊善柏,张声华.2002稻米淀粉的理化特性研究Ⅰ不同类型稻米淀粉的理化特性.中国粮油学报,17(6):39-42.
赵学伟,卞科,王金水.1997.蛋白质与淀粉的相互作用对陈化大米质构特性的影响.郑州粮食学院学报,3:23-25.
赵学伟,卞科,王金水.2001.陈米质构特性变化机理研究进展.郑州工程学院学报,22(2):5-9.
郑国华,黄会萱,胡友战,韦启鹏,蒋云生.2000.稻谷型饲粮养猪研究.见:韩友文,主编.中国畜牧兽医学会动物营养学分会第六届全国会员代表大会暨第八届学术研讨会论文集.哈尔滨:黑龙江人民出版社,pp 256-259.
郑艺梅,何瑞国.2001.早稻糙米蛋白质的评价.粮食与饲料工业,12:5-6.
郑艺梅、何瑞国、蔡道明、隗德合.2002a.不同品种早稻糙米养分利用率的研究.中国粮油学报,17(1):38-42.
郑艺梅,何瑞国,徐三平,蔡道明.2002b.不同品种早稻糙米营养成分含量的分析.中国粮油学报,17(6):44-47.
周惠明.1996.糙米加工技术研究.粮食与饲料工业,3:1-4.
周惠明,张民平.2002.糙米中功能性成分的研究.食品科技,5:17-19.
AACC. 2000. Approved methods of the American Association of Cereal Chemists 10~(th) Ed. AACC Inc. Washington, DC.
AOAC. 1990. Official Methods of Analysis 15~(th) Ed. Association of Official Analytical Chemists. Washington. DC.
Baidoo, S. K., Y. G Liu, and D. Yungblut. 1998. Effects of microbial enzyme supplementation on energy, amino acid digestibility and performance of pigs fed hulless barley based diets. Can. J. Anim. Sci. 78: 625-631.
Bedford, M. R., and H. L. Classen. 1992. Reduction of intestinal viscosity through manipulation of dietary rye and pentosanase concentration is effected through changes in the carbohydrate composition of intestinal aqueous phase and results in improved growth rate and food conversion efficiency of broiler chicks. J. Nutr. 122:560-569.
Bird, A. R., T. Hayakawa, Y. Marsono, J. M. Gooden, I. R. Record, R. L. Correll, and D. L. Topping. 2000. Coarse brown rice increases fecal and large bowel short-chain fatty acids and starch but lowers calcium in the large bowel of pigs. J. Nutr. 130: 1780-1787.
Brown, J. A., and T. R. Cline. 1974. Urea excretion in the pig: an indicator of protein quality and amino acid requirements. J. Nutr. 104: 542-545.
Cera, K. R., and G. A. Reinhart. 1990. Effect of weaning, week post-weaning and diet composition on pancreatic and small intestinal lumial lipase response in young swine. J. Anim. Sci. 68:384-391.
Chae, B. J., H. I. Kang, In. K. Han, J. H. Kim, W. T. Cho, Y. K. Chung, and M. S. Shim. 1998. The effect of maize particle size and pellet size on growth and carcass traits in growing-finishing pigs. Kor. J. Anim. Nutr. Feed. 22:81-86.
Chappie, R. P., J. A. Cuaron, and R. A. Easter. 1989. Response of digestive carbohydrase and growth to graded doses and administration frequency of hydrocortisone and adrenocorticotropic hormone in nursing piglets. J. Anim. Sci. 67:2974-2984.
Choct, M., and G. Annison. 1992. Anti-nutritive effect of wheat pentosans in broiler chickens: role of viscosity and gut microflora. Br. Poult. Sci. 33:821-834.
Chung, Y. K., B. J. Chae, J. H. Kim, K. S. Chu, and In. K. Han. 1998. An evaluation of barley in finishing pig diets for high quality pork production. Kor. J. Anim. Nutr. Feed. 22:15-19.
Cranwell, P. D., D. E. Noakes, and K. J. Hill. 1976. Gastric Secretion and fermentation in the suckling pig. Br.J. Nutr. 36:71-86.
Dahlqvist, A. 1961. Intestinal carbohydrase of a new-born pig. Nature 190 :31-32.
Dahlqvist, A. 1964. Method for assay of intestinal disaccharidase. Anal. Chem. 7:18-25.
De Lange, C. F. M., W. C. Sauer, R. Mosenthin, and W. B. Souffrant. 1989. The effect of feeding different protein-free diets on the recovery and amino acid composition of endogenous protein collected from distal ileum and feces in pigs. J. Anim. Sci. 67:746-754.
Efird, R. C, W. D. Armstrong, and D. L. Herman. 1982. The development of digestive capacity in young pigs: effects of weaning regimen and dietary treatment. J. Anim. Sci. 55:1370-1379.
Englyst, H. N., and J. H. Cummings. 1985. Digestion of the polysaccharides of some cereal foods in the human small intestine. Am. J. Clin. Nutr. 42:778-787.
Englyst, H. N., and J. H. Cummings. 1987. Digestion of polysaccharides of potato in the small intestine of man. Am. J. Clin. Nutr. 45:423-431.
Fan, M. Z., W. C. Sauer, R. T. Hardin, and K. A. Lien. 1994. Determination of apparent ileal amino acid digestibility in pigs: effect of dietary amino acid level. J. Anim. Sci. 72:2851-2859.
Farrell, D. J., and K. Hutton. 1990. Rice and rice milling by-products. In: P. A. Thacker and R. N. Kirkwood (ed.) Nontraditional Feed Sources for Use in Swine Production. Stoneham: Butterworth & Co. Publishers Ltd., pp 339-353.
Goda, T, and S. Takase. 1994a. Dietary carbohydrate and fat independently modulate dissaccharidase activities in rat jejunum. J. Nutr. 124:2233-2239.
Goda, T., T. Urakawa, M. Watanabe, and S. Takase. 1994b. Effect of high amylose starch on carbohydrate digestive capability and lipogenesis in epididymal adipose tissue and liver of rats. J.
Nutr. Biochem. 5:256-260.
Goda, T., H. Yasutake, T. Tanaka, and S. Takase. 1999. Lactase-phlorizin hydrolase and sucrase-isomaltase genes are expressed differently along the villus-crypt axis of rat jejunum. J. Nutr. 129:1107-1113.
Graham, H., J. G. Fadel, C. W. Newman, and R. K. Newman. 1989. Effect of pelleting and β-glucanase supplementation on the ileal and fecal digestibility of a barley-based diet in the pig. J. Anim. Sci. 67:1293-1298.
Gray, M. G 1992. Starch digestion and absorption in nonruminants. J. Nutr. 122:172-177.
Green, S., S. L. Bertrand, M. J. C. Duron, and R. A. Maillard. 1987. Digestibility of amino acids in maize, wheat and barley meal, measured in pigs with ileo-rectal anastomosis and isolation of the large intestine. J. Sci. Food Agric. 41:29-43.
Hampson, D. J., and D. E. Kidder. 1986. Influence of creep feeding and weaning on brush border enzyme activities in the piglet small intestine. Res. Vet. Sci. 40: 24-31.
Hancock, J. D., R. H. Hines, G. E. Fitzner, and T. L. Gugle. 1991. Effect of extrusion processing on the nutritional value of sorghum and soybeans for finishing pigs. In: Proceedings of 17th Biennial Grain Sorghum Utilization Conference. USA: Lubbock, TX, pp 52-59.
Henning, S. J. 1985. Ontogeny of enzymes in the small intestine. Ann. Rev. Physiol. 47:231-245.
Hesselman, K., and P. Aman. 1986. The effect of beta-glucanase on the utilization of starch and nitrogen by broiler chickens fed on barley of low or high viscosity. Anim. Feed Sci. Technol. 15:83-93.
Hildebrandt, L. A., and J. A. Marlett. 1991. Starch bioavailability in the upper gastrointestinal tract of colectomized rats. J. Nutr. 121:679-686.
Inborr, J., M. Nasi, and K. Suomi. 1988. Effect of enzyme treatment of cooked barley and supplementation of piglet diets on the digestibility of barley and piglet performance. J. Agric. Sci. 60:685-699.
Inborr, J., M. Schmitz, and F. Ahrenes. 1993. Effect of adding fibre and starch degrading enzymes to a barley/wheat based diet on performance and nutrient digestibility in different segments of the small intestine of early weaned pigs. Anim. Feed Sci. Technol. 44:113-127.
Jensen, M. S., S. K. Jensen, and K. Jakobsen. 1997. Development of digestive enzymes in pigs with emphasis on lipolytic activity in the stomach and pancreas. J. Anim. Sci. 75:437-445.
Karkalas, J. J. 1985. An improved enzymatic method for the determination of native and modified starch. J. Sci. Food Agric. 36:1019-1027.
Kelly, D., J. A. Smyth, and K. J. McCracken. 1991. Digestive development of the early-weaned pig. 1.
Effect of continuous nutrient supply on the development of the digestive tract and on changes in digestive enzyme activity during first week post-weanling. Br. J. Nutr. 65:169-180.
Kidder, D. E., and M. J. Manners. 1980. The level and distribution of carbohydrase in the small intestine mucosa of pigs from 3 weeks of age to maturity. Br. J. Nutr. 43:141-153.
Kim, I. H., J. D. Hancock, L. Burnham, G A. Kennedy, R. H. Hines, K. C. Behnke, and D. A. Nichols. 1995. Processing procedures and feeding system for sorghum-based diets given to lactating sows. J. Anim. Sci. 73 (Suppl. 1) :185. (Abstr.)
Koldovsky, O. 1981. Developmental, dietary, and hormonal control of intestinal disaccharidases in mammals (including man). In: Frank Dickens, P. J. Randle, and W. J. Whelan (ed.) Carbohydrate Metabolism and Disorders. New York: Academic Press, pp 482-522.
Li, D. F, R. C. Thaler, J. L. Nelssen, D. L. Harmon, G L. Allee, and T. L. Weeden. 1990. Effect of fat sources and combinations on starter pig performance, nutrient digestibility and intestinal morphology. J. Anim. Sci. 68:3694-3704.
Li, D. R, D. F. Zhang, X. S. Piao, In. K. Han, J. B. Li, and J. H. Lee. 2002. Effects of replacing com with Chinese brown rice on growth performance and apparent fecal digestibility of nutrients in weanling piglets. Asian-Aust. J. Anim. Sci. 15:1191-1197.
Lin, R D., D. A. Knabe, and T. D. Jr. Tanksley. 1987. Apparent digestibility of amino acids, gross energy and starch in corn, sorghum, wheat, barley, oat groats and wheat middlings for growing pigs. J.Anim. Sci. 64:1655-1663.
Lindemann, M. D., S. G Cornelius, S. M. E. Kandelgy, R. L. Moser, and J. E. Pettigrew. 1986. Effect of age, weaning and diet on digestive enzyme level in the piglet. J. Anim. Sci. 62:1298-1307.
Makkink, C. A., J. M. Berntsen, B. M. L. Kamp, B. op den Kemp, and M. W. A. Versteqen. 1994. Gastric protein breakdown and pancreatic enzyme activities in response to two different dietary protein sources in newly weaned pigs. J. Anim. Sci. 72:2843-2850.
Manners, M. J., and J. A. Stevens. 1972. Changes from birth to maturity in the pattern of distribution of lactase and sucrase activity in the mucosa of the small intestine of pigs. Br. J. Nutr. 28:113-127.
Marsono, Y., R. J. Illman, J. M. Clarke, R. P. Trimble, and D. L. Topping. 1993. Plasma lipids and large bowel volatile fatty acids in pigs fed on white rice, brown rice and rice bran. Br. J. Nutr. 70:503-513.
Mercier, C, and P. Feillet. 1975. Modification of carbohydrate components by extrusion-cooking of cereal products. Cereal Chem. 52:283-287.
Miller, J. B., E. Pang, and L. Bramall. 1992. Rice: a high or low glycemic index food? Am. J. Clin. Nutr. 56:1034-1036.
NRC. 1998. Nutrient requirements of swine 10th Ed. National Academy Press, Washington, DC.
Owsley, W. R, D. E. Jr. Orr, and L. F. Tribble. 1986. Effect of age and diet on the development of the pancreas and the synthesis and secretion of pancreatic enzymes in the young pig. J. Anim. Sci. 63: 497-504.
Perozzi, G, D. Barila, C. Murgia, D. Kelly, R. Begbie, and T. P. King. 1993. Expression of differentiated functions in the developing porcine small intestine. J. Nutr. Biochem. 4:699-705.
Piao, X. S., D. F. Li, In. K. Han, Y. Chen, J. H. Lee, D. Y. Wang, J. B. Li, and D. F. Zhang. 2002. Evaluation of Chinese brown rice as an alternative energy source in pig diets. Asian-Aust. J. Anim. Sci. 15: 89-93.
Piao, X. S., B. J. Chae, J. H. Kim, J. Jin, W. T. Cho, and In. K. Han. 1999a. Effects of extrusion condition of barley on growth performance, fecal digestibility and reductions in nutrient excretion in growing pigs. Asian-Aust. J. Anim. Sci. 12:783-787.
Piao, X. S., In. K. Han, J. H. Kim, W. T. Cho, Y. H. Kim, and C. Liang. 1999b. Effects of Kemzyme, Phytase and Yeast supplementation on the growth performance and pollution reduction of broiler chicks. Asian-Aust. J. Anim. Sci. 12:36-41.
Pierzynowski, S. G, B. R. Westrom, B. W. Karlsson, J. Svendsen, and B. Nilsson. 1988. Pancreatic cannulation of young pigs for long-term study of exocrine pancreatic function. Can. J. Anim. Sci. 68:953-959.
Sangild, P. T., P. D. Cranwell, H. Sorensen, K. Mortensen, O. Noren, L. Wetteberg, and H. Sjostrom. 1991. Development of intestinal disaccharidases, intestinal peptidases and pancreatic proteases in sulking pigs, the effect of age and ACTH treatment. In: Vertegen, M. W. A., J. Huisman and den L. A. Hartog (ed.) Digestive Physiology in Pigs. Wageningen: Pudoc Press, pp 73-78.
Sauer, W. C. 1976. Factors influencing amino acid availability for cereal grains and their components for growing monogastric animals. Ph. D. Thesis, University of Manitoba, Winnipeg, Manitoba.
Sauer, W. C, J. J. Kennelly, F. X. Aherne, and R. M. Cichon. 1981. Availabilities of amino acids in barley and wheat for growing pigs. Can. J. Anim. Sci. 61:793-802.
Sauer, W. C, and L. Ozimek. 1986. Digestibility of amino acids in swine: results and their practical application: a review. Livest. Prod. Sci. 15:367-388.
Schneeman, B. O. 1978. Effect of plant fiber on lipase, trypsin and chymotrypsin activity. J. Food Sci. 43:634-635.
Sell, J. L., O. Koldovsky, and B. L. Reid. 1989. Intestinal disaccharidase of young turkeys: temporal development and influence of diet composition. Poult. Sci. 68:265-277.
Shields, R. G. Jr., K. E. Ekstrom, and D. C. Mahan. 1980. Effect of weaning age and feeding method in
digestive enzyme development in swine from birth to ten weeks, J. Anim Sci 50:257-265.
Siddons, R. C, and M. E. Goates. 1972. The influence of the intestinal microflora on dissaccharidase activities in the chick. Br. J. Nutr. 27:101-112.
Solimano, G, E. Burgess, and B. Levin. 1967. Effect of protein-calorie malnutrition deficient diet on enzyme levels of jejunal mucosa of rats. Br. J. Nutr. 21:51-58.
SPSS Inc., 1998. SPSS for Windows Base System User's Guide Release 9.0. SPSS Inc., Chicago, IL. USA.
Suga, Y., M. Kawai, S. Noguchi, G Shimura, and H. Samejima. 1978. Application of cellulolytic and plant tissue macerating enzyme of irpex lacteus fr. as feed additive enzyme. Agric. Biol. Chem. 42:347-350.
Todesco, T., A. V. Rao, O. Bosello, and D. J. Jenkins. 1991. Propionate lowers blood glucose and alters lipid metabolism in healthy subjects. Am. J. Clin. Nutr. 54:860-865.
Uni, Z., Y. Noy, and D. Sklan. 1999. Post-hatch development of small intestine function of the poultry. Poult. Sci. 78:215-222.
Weurding, R. E. 2002. Kinetics of starch digestion and performance of broiler chickens. Ph. D. Thesis, Wageningen University, Wageningen, The Netherlands.
Yin, Y. L., S. K. Baidoo, L. Z. Jin, Y.G Liu, H. Schulze, and P. H. Simmins. 2001a. The Effect of different carbohydrase and protease supplementation on apparent (ileal and overall) digestibility of nutrients of five hulless barley varieties in young pigs. Livest. Prod. Sci. 71:109-120.
Yin, Y. L., S. K. Baidoo, H. Schulze, and P. H. Simmins. 2001b. Effects of supplementing diets containing hulless barley varieties having different levels of non-starch polysaccharides with beta-glucanase and xylanase on the physiological status of the gastrointestinal tract and nutrient digestibility of weaned pigs. Livest. Prod. Sci. 71:97-107.
Yin, Y. L., J. D. McEvoy, H. Schulze, and K. J. McCracken. 2001c. Effects of xylanase and antibiotic addition on ileal and overall apparent digestibilities of dietary nutrients and evaluating HCl-insoluble ash as a dietary marker in growing pigs. Anim. Sci. 72:95-103.
Yin, Y. L., J. McEvoy, H. Schulze, W. B. Souffrant, U. Hennig, and K. J. McCracken. 2000. Apparent digestibility (ileal and overall) of nutrients and endogenous nitrogen losses in growing pigs fed wheat (var soissons) or its by-products without or with xylanase supplementation. Livest. Prod. Sci. 62:119-132.
Zhang, D. F, D. F. Li, X. S. Piao, In. K. Han, C. J. Yang, J. G Dai, and J. B. Li. 2002. Effects of replacing corn with brown rice or brown rice with enzyme on growth performance and nutrient
digestibility in growing pigs. Asian-Aust. J. Anim. Sci. 15:1334-1340.