生精汤对阳虚精亏型弱精子活力影响的研究
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摘要
目的：观察中药生精汤对阳虚精亏型弱精子症患者精液参数中精子活力及弱精子症阳虚精亏证型中医症状评分表积分的影响,并观察药物干预中是否出现不良反应。方法：按照病例纳入及排除标准在新疆医科大学附属中医医院男科就诊的弱精子症患者中选取符合标准的受试对象并辨证为阳虚精亏证型。按照随机的原则将纳入病例分为治疗组和对照组。治疗组和对照组各取30例。治疗组为口服生精汤,每日一剂,每剂煎服两次,每天早、晚饭后半小时口服。对照组为口服左卡尼汀口服溶液,每次10ml,每天早、晚饭后半小时口服。左卡尼汀口服溶液系东维力牌,由东北制药总厂生产。治疗组和对照组均于服药前及服药三个月后进行一次精液分析,记录其精液参数中的精子活力。并于治疗前和治疗后对患者进行阳虚精亏证型中医症状评分表积分。结果：经过对数据统计分析后,得出结果：实验组治疗前后精子活力比较,差异有统计学意义(P<0.01),两组比较,实验组治疗后精子活力提升优于对照组,差异有统计学意义(P<0、05)；中医症状评分表积分显示,经生精汤干预后,实验组中医症状评分表积分低于对照组(P<0.01),具有改善中医症状的效果。在年龄与疗效关系比较中,23岁-26岁、27岁～30岁、31岁-34岁三个年龄段的总有效率均高于35岁-39岁年龄段,提示年龄段低者疗效优于年龄段高者。在病情分级与疗效关系比较中,病情分级中轻度和中度患者的总有效率高于重度患者,提示病情分级轻者疗效优于病情分级重者。治疗组和对照组单项中医症状比较,经统计分析发现：治疗组在改善症状头晕耳鸣、形寒肢冷、小便清长、腰膝酸软四症方面,效果优于对照组,差异有统计学意义(P<0.01)。而治疗组在改善症状发落齿摇方面,效果亦优于对照组,差异有统计学意义(P<0.05)。结论：中药生精汤可以改善阳虚精亏型弱精子症患者精液参数中的精子活力,并可以改善阳虚精亏型弱精子症患者中医临床症状,且不良反应少。
Objective:Observation of TCM Sheng Jing Decoction fine kui Yang type Asthenospermia in patients with semen parameters in sperm motility and Asthenospermia disease pure kui Yang syndrome type TCM symptoms scoresheets integral effect, and observe whether pharmacological interentions appear harmfulresponse. Methods:Accord-ing to the cases into and exclusion standard in xinjiang medical university hospital affiliated hospital of traditional male Asthenospermia in patients select the standard test subjects and dialectical for pure kui Yang syndrome type. In random principle will test subjects divided into the treatment group and control group. Treatment group and control group,30 cases from each. Oral treatment group is born a daily dose of pure soup, each agent Fried taken twice, everyday early, after supper half an hour oral. The control group for oral left karni Hastings oral solution, every time 10ml, every day early, after supper half an hour oral. Left karni Hastings oral solution is east dimension force CARDS, from the northeast to general pharmaceutical factory, production. Treatment group and control group in all baseline and three months after taking a semen analysis, record its semen parameters of sperm motility. And in treatment before and after treatment for patients with pure kui Yang syndrome type TCM symptoms scoresheets integrals. Results:Through the statistical analysis of the data, it is concluded that the results:the treatment before-and-after sperm motility comparison, difference was statistically significant (P<0.01), two groups of comparisons, group after the treatment of sperm motility ascension than control group. Difference was statistically significant (P<0,05), TCM symptoms scoresheets integral are displayed via pure soup interentions, born in experimental TCM symptoms scoresheets points lower than that in control group (P<0.01), can improve the effect of TCM symptoms. In age and curative effect relation in the comparison,23-26,27-30, 31-34 three age groups of the total effective rate are higher than 35-39 years of age, suggests that the lower the age better therapeutic ages. The disease classification and treatment relationship in the comparison, the illness in patients with mild to moderate classification total effective rate higher than patients with seere disease, suggests that the grading light person better therapeutic disease classification is weighed. Treatment group and control group by single TCM symptoms comparison, analysis found that:treatment group in improve symptoms dizziness tinnitus, cold extremities, cold and urinate fractal qing long, lumbar debility four disease, effect than in control group, difference was statistically significant(P< 0.01). And in treatment group improve symptoms let tine shakes, effect also better than control group, and the difference was statistically significant(P<0.05). Conclusion:Chinese medicine Sheng Jing Decoction can improve pure kui Yang type Asthenospermia in patients with semen parameters of sperm motility, and can improve the pure kui Yang type Asthenospermia in patients with TCM clinical symptoms, and fewer side effects.
Objective:Observation of TCM Sheng Jing Decoction fine kui Yang type Asthenospermia in patients with semen parameters in sperm motility and Asthenospermia disease pure kui Yang syndrome type TCM symptoms scoresheets integral effect, and observe whether pharmacological interentions appear harmfulresponse. Methods:Accord-ing to the cases into and exclusion standard in xinjiang medical university hospital affiliated hospital of traditional male Asthenospermia in patients select the standard test subjects and dialectical for pure kui Yang syndrome type. In random principle will test subjects divided into the treatment group and control group. Treatment group and control group,30 cases from each. Oral treatment group is born a daily dose of pure soup, each agent Fried taken twice, everyday early, after supper half an hour oral. The control group for oral left karni Hastings oral solution, every time 10ml, every day early, after supper half an hour oral. Left karni Hastings oral solution is east dimension force CARDS, from the northeast to general pharmaceutical factory, production. Treatment group and control group in all baseline and three months after taking a semen analysis, record its semen parameters of sperm motility. And in treatment before and after treatment for patients with pure kui Yang syndrome type TCM symptoms scoresheets integrals. Results:Through the statistical analysis of the data, it is concluded that the results:the treatment before-and-after sperm motility comparison, difference was statistically significant (P<0.01), two groups of comparisons, group after the treatment of sperm motility ascension than control group. Difference was statistically significant (P<0,05), TCM symptoms scoresheets integral are displayed via pure soup interentions, born in experimental TCM symptoms scoresheets points lower than that in control group (P<0.01), can improve the effect of TCM symptoms. In age and curative effect relation in the comparison,23-26,27-30, 31-34 three age groups of the total effective rate are higher than 35-39 years of age, suggests that the lower the age better therapeutic ages. The disease classification and treatment relationship in the comparison, the illness in patients with mild to moderate classification total effective rate higher than patients with seere disease, suggests that the grading light person better therapeutic disease classification is weighed. Treatment group and control group by single TCM symptoms comparison, analysis found that:treatment group in improve symptoms dizziness tinnitus, cold extremities, cold and urinate fractal qing long, lumbar debility four disease, effect than in control group, difference was statistically significant(P< 0.01). And in treatment group improve symptoms let tine shakes, effect also better than control group, and the difference was statistically significant(P<0.05). Conclusion:Chinese medicine Sheng Jing Decoction can improve pure kui Yang type Asthenospermia in patients with semen parameters of sperm motility, and can improve the pure kui Yang type Asthenospermia in patients with TCM clinical symptoms, and fewer side effects.
引文
[1]Cooper TG, Noonan E, Von Eckardstein S, et al. World Health Organization reference values for human semen Characteristics. Hum Reprod Update2009;[Epub ahead of print]
[2]Check JH. Treatment of male infertility. Clin Exp Obstet Gynecol 2007;34(4):201-206
[3]Dada R, Gupta NP, Kucheria K. Molecular screeningfor Yq microdeletionin men with idiopathic oligozoospermia and azoospermia[J]. J Biosci,2003,28(2):163-168.
[4]Dada R, Gupta NP, Kucheria K. Molecular screening for Yq microdeletionin men with idiopathic oligozoospermia and azoospermia[J]. J Biosci,2003,28(2):163-168.
[5]郭应禄李宏军主编.男性不育症[M].第1版.北京：人民军医出版社,2003.160-166.
[6]世界卫生组织.人类精液及精子—宫颈粘液相互作用实验室检验手册[M].第4版.北京：人民卫生出版社,2001.3-8.
[7]国家技术监督局发布.中华人民共和国国家标准—中医临床诊疗术语证候部分.1997.38-39.
[8]世界卫生组织.不育夫妇标准检查与诊断手册[M].北京：科学出版社,1994.19.
[9]中华人民共和国卫生部.中药新药临床研究指导原则.第1辑[S].1993.192.
[10]樊友平,朱佳卿,李家蓉,等主编,《中华泌尿男科学古典集成》[M].第1版.北京,中医古籍出版社,2007
[11]贾金铭主编.中国中西医结合男科学[M].第1版.北京.中国医药科技出版社,2005,176-178
[12]王琦主编.男科疾病中西医汇通[M].第1版.沈阳：辽宁科学技术出版社,2003.112.
[13]倪良玉.徐福松教授治疗男性不育症经验[J].中医研究,2007,20(12)：43-44.
[14]李日庆.男性不育症中西医治疗概况[J].现代诊断与治疗,2008,19(3)：129-133.
[15]孙自学,陈建设.王琦教授治疗男性不育经验介绍[J].四川中医,2004,22(1)：7-8.
[16]冯德海.郭军教授治疗男性不育症经验总结[J].四川中医,2007,25(6)：7-8.
[17]廖敦,刘志刚.西洋参治疗弱精子症62例疗效观察[J].中国实用医药,2009,5.4(15)：174-175
[18]吴维,刘继红,尹春萍,等.刺五加注射液与茶碱、咖啡因体外对精子运动功能影响的比较研究[J].中华男科学杂志,2009,15(3)：278-281
[19]张燕,郭贤坤,刘汉丹.黄芪注射液体外对精子运动相关参数的影响[J].中药材,2010.7,33(3)：485-486
[20]仇仁良,周惠耕.人参治疗弱精子症不育疗效观察[J].现代中西医结合杂志,2008,4.7(10)：1515
[21]孟庆杰,马啸,郭通航.复方玄驹胶囊联合锌硒宝片对少弱畸形精子症患者精子质量的影响[J].生殖与避孕,2010,7.30(7)：502-503
[22]郭应禄李宏军主编.男性不育症[M].第1版.北京：人民军医出版社,2003.
[23]王维平,汪晶冰,马洪星,等.292例男性少、弱精子症和无精子症患者的细胞遗传学分析[J].中国优生与遗传杂志,2010,18(7)：43
[24]袁慧珍,刘艳秋.男性不育的细胞遗传学分析[J].江西医药,2009,3.44(3)：243-245
[25]陈德宇,刘利敏,谢庆东,等.弱精症患者精子基因表达谱的建立及分析[J].癌变畸变突变,2009,7.21(4)0286-0290
[26]崔艳国,张立新,董丽萍,等.男性低生育人群精浆锌、酸性磷酸酶及血清性激素水平及意义[J].山东医药,2010,50(25)：85-86
[27]宋博,蔡志明.雄激素与精子发生的关系[J].临床泌尿外科杂志,2008，7.23(7)：553-555
[28]郑洁,何彦芳,李俊林.人精子中芳香化酶表达与精子运动能力[J].中国男科学杂志,2009,23(8)：22-24
[29]周其赵,冯春琼,毛向明.弱精子症相关基因与蛋白研究进展[J].中华男科学杂志,2009,15(9)：836-839
[30]Ferlin A, Vinanzi C, Garolla A, et al. Clin Endocrinol(Oxf)2006;65(5):606-610
[31]金龙金,李传连,费前进,等.mtATPase6基因变异与弱精子症的相关分析[J].细胞生物学杂志,2008,30：660-666.
[32]冯春琼,宋艳斌,邹亚光,等.弱精子症患者精子线粒体MTCYB.MTATP6基因的检测[J].中国男科学杂志,2008,14(4)：321-323
[33]李传连,楼哲丰,黄学锋,等.线粒体基因ND3.ND4L核苷酸变异与弱精子症相关分析[J].中国病理生理杂志,2010,26(2)：362-367
[34]Minocherhomji S, Athalye AS, Madon PF, et al. A case-control study identifying chromosomal polymorphic variations as forms of epigenetic alterations associated with the infertility phenotype. Fertil Steril 2009;92(1):88-95
[35]张清健,郑立新,田佩玲,等。精液分析异常患者的染色体分析[J].中国男科学杂志,2010,24(7)：51-53
[36]李练兵,夏彦恺,李新生,等.弱精子症患者精子X,Y，18号染色体的三色荧光原位杂交分析[J].中国男科学杂志,2008,14(3)：211-214.
[37]Appasamy M, Muttukrishna S, Pizzey AR, et al. Relationship between male reproductive hormones, sperm DNA damage and markers of oxidative stress in infertility. Repord Biomed Online 2007;14(2):159-165
[38]Zhang Y, Wang H, Wang LR, et al. The clinical significance of sperm DNA damage detection combined with routin semen testing in assisted reproduction. Molecular Medcine Reports 2008; 1:617-624
[39]孙静波,姜宏,何瑞冰.精子DNA完整性与精液参数的相关性研究[J].中国男科学杂志,2010,24(4)：26-29
[40]Makkerk, Agarwal A, Sharma R. Oxidative stress & male infertility. Indian J Med Res 2009;129(4):357-367
[41]Mehcli M, Khantouche L, Ajina M, et al. Detection of DNA fragmentation in human spermatozoa:correlation with semen parameters. Andrologia 2009;41(6):383-386
[42]Shamsi MB, Venkstesh S, Tanwar M, et al. DNA integrity and semen quality in men with low seminal antioxidant levels. Mutat Res 2009;665(1-2)29-36
[43]Kumar R, Venkatesh S, Kumar M, et al. Oxidative stress and sperm mitochondrial DNA mutation in idiopathic oligoasthenozoospermic men. Indian J Biochem Biophys 2009;46(2):172-177
·-[44]曹兴午,李翠英.线粒体缺失与不育症[J].中国男科学杂志,2010；24(5)：71-72
[45]Rahman MM, Laher I. Structural and functional alteration of blood vessels caused by cigarette smoking:an overview of molecular mechanisms. Curr Vase. Pharmacol 2007;5(4):276-292
[46]Shamsi MB, Venkstesh S, Tanwar M, et al. DNA integrity and semen quality in men with low seminal antioxidant levels. Mutat Res 2009;665(1-2)29-36
[47]赵洪鑫,史庭燕,时伟丽,等.应用化学发光法检测男性不育人群精液活性氧水平[J].中国男科学杂志,2009,23(4)：14
[48]刘居理,罗明,卢雪芳,等.少弱精子症、弱精子症患者精浆活性氧浓度检测及其应用价值.中国实验诊断学,2009,9,13(9)：1275-1276
[49]罗胜萍,吴蓉,刘自刚.男性不育患者精浆锌含量的变化分析[J].中国实验诊断学,2009,6.13(6)：828-829
[50]张睿.男性少弱精子症患者精浆锌含量分析[J].临床研究,2010,5.48(15)：29-30
[51]Espino J, Mediero M, Lozano GM, et al. Reduced levels of intracellular calcium releasing in spermatozoa from asthenozoospermic patients. Reprod Biol Endocrinol 2009;7:11
[52]薛炜,邢俊平.弱精子症患者与正常精子人群精子相比多不饱和脂肪酸减少,饱和脂肪酸含量增加[J].现代泌尿外科杂志,2008,13(1)：
[53]杨晓华,耿翠艳.少、弱、畸形精子症患者血铅、镉浓度的测定[J].中国男科学杂志,2008,22(9)：53-55.
[54]张宁,汤洁,鲁海鸥,等。低强度微波辐射和低频噪声对男性精子影响的流行病学调查研究[J]。中国男科学杂志,2007,21(6)：23-25,29
[55]Jacosson B, Aaltonen R, Rantakokko-Jalava K, et al. Quantification of Ureaplasma urealyticum DNA in the amniotic fluid from patients in PTL and PPROM and its relation to inflammatory cytokine levels. Acta Obstet Gynecol Scand 2009;88(1):63-70
[56]徐峥,白云,谭巨莲,等.解脲支原体感染患者精浆中细胞因子对精子活力的影响[J].中国男科学杂志,2010,24(5)：30-32
[57]邵欣胤,李金彪,朱琳.体外精浆置换对弱精子症患者精子运动能力的影响[J].山东医药,2010,50(6)：51-52
[58]郭应禄李宏军主编.男性不育症[M].第1版.北京：人民军医出版社,2003.226-229
[59]Balercia G, Regoli F, Armeni T, et al.. Placebo-controlled double-blind randomized trial on the use of L-carnitine, L-acetylcarnitine, or combined L-carnitine and L-acetylcarnitine in men with idiopathic asthenozoospermia[J]. Fertil Steril,2005, 84(3):662-671.
[60]Ebisch IM, Thomas CM, Peters WH, et al. The important of folate, zinc and antioxidants in the pathogenesis and prevention of subfertility[J]. Hum Reprod Update, 2007,13(2):163-174.
[61]Balercia G, Mosca F, Mantero F, et al. Coenzyme Q(10)supplementation in infertile men with idiopathic asthenozoospermia:an open, uncontrolled pilot study[J]. Fertil Steril,2004,81(1):93-98.
[62]瞿利军.口服西地那非或他达拉非改变不育症男性的精子活力[J].中华男科学杂志,2008,14(1)：92.
[63]卢少明,李晓,张浩波,等.克罗米芬联合己酮可可碱治疗特发性少、弱精子症的临床研究[J].中国男科学杂志,2008,22(5)：39-41.
[64]阮衍泰.它莫西芬配合金施尔康、肾肾气丸对精液质量的改善[J].现代泌尿外科杂志,2009,1.14(1)：32-34
[65]嵇文田,田洪志,赵增涛,等.锌硒宝联合左卡尼汀治疗特发性弱精子症120例分析[J].生殖与避孕,2008,28(10)：639,634.
[66]张利,段晓明,徐计秀,等.左旋肉碱加五子衍宗丸治疗少弱精子症[J].中国中西医结合外科杂志,2010,10.16(5)：535-537
[67]卜律花.多维元素配合IMC治疗少精弱精症的疗效观察[J].中国计划生育学杂志,2009，7.165：434-435
[68]徐波,付伟.弱精子症的中医证治研究[J].中国当代医药,2009,3.16(5)：58-60
[69]申树林,赵倩,徐刚,等.养精汤治疗特发性弱精子症的疗效观察[J].中华男科学杂志,2010,16(6)：569-57
[70]Ladachart Taepongsorat, Prakong Tangpraprutgul, Noppadon Kitana, et al. Stimulating effects of quercetin on sperm quality and reproductive organs in adult male rats. Asian J Androl 2008;10(2):249-258
[71]钟百灵.愈精颗粒治疗男性不育症药效学研究[J].山东中医杂志,2002,21(3)：177-179.
[72]颜正华主编.中药学[M].第2版.北京.人民卫生出版社.2006.
[73]王本祥主编.现代中药药理与临床[M].第1版.天津.天津科技翻译出版公司,2004.
[1]王琦主编.男科疾病中西医汇通[M].第1版.沈阳：辽宁科学技术出版社,2003.112.
[2]杨建华主编.实用男性不育诊疗学[M].第1版.上海：第二军医大学出版社,2002.178-180.
[3]郭雪华,郑连文.弱精子症的研究进展[J].中国男科学杂志,2008,(22)6：54-56.
[4]金龙金,李传连,费前进,等.mtATPase6基因变异与弱精子症的相关分析[J].细胞生物学杂志,2008,30：660-666.
[5]冯春琼,宋艳斌,邹亚光,等.弱精子症患者精子线粒体MTCYB、MTATP6基因的检测[J].中国男科学杂志,2008,14(4)：321-323.
[6]李练兵,夏彦恺,李新生,等.弱精子症患者精子X,Y，18号染色体的三色荧光原位杂交分析[J].中国男科学杂志,2008,14(3)：211-214.
[7]薛炜,邢俊平.弱精子症患者与正常精子人群精子相比多不饱和脂肪酸减少,饱和脂肪酸含量增加[J].现代泌尿外科杂志,2008,13(1)：
[8]杨晓华,耿翠艳.少、弱、畸形精子症患者血铅、镉浓度的测定[J].中国男科学杂志,2008,22(9)：53-55.
[9]孙自学,陈建设.王琦教授治疗男性不育经验介绍[J].四川中医,2004,22(1)：7-8.
[10]李曰庆.男性不育症中西医治疗概况[J].现代诊断与治疗,2008,19(3)：129-133.
[11]倪良玉.徐福松教授治疗男性不育症经验[J].中医研究,2007,20(12)：43-44.
[12]冯德海.郭军教授治疗男性不育症经验总结[J].四川中医,2007,25(6)：7-8.
[13]胡廉,熊承良.野山楂根含药血清对弱精子症患者精子运动参数影响的体外研究[J].中国中药杂志,2006,31(4)：333-335.
[14]李忠远,陈智.刺五加水提物对人精子体外运动活力的影响[J].医药导报,2006,25(5)：387-389.
[15]卢太坤.虎杖丹参饮治疗免疫性不育男性的临床研究[J].中华男科学杂志,2006,12(8)：750-755.
[16]张俊慧,王楠,曹爽,等.枸杞子在男性不育症中的应用[[J].中华男科学杂志,2008,14(3)：279-281.
[17]郭应禄李宏军主编.男性不育症[M].第1版.北京：人民军医出版社,2003.160-166.
[18]程怀瑾,陈廷.左旋肉碱和乙酰左旋肉碱复合制剂对特发性弱精子症精子质量的影响[J].中华男科学杂志,2008,14(2)：149-151.
[19]Balercia G, Regoli F, Armeni T, et al.. Placebo-controlled double-blind randomized trial on the use of L-carnitine, L-acetylcarnitine, or combined L-carnitine and L-acetylcarnitine in men with idiopathic asthenozoospermia[J]. Fertil Steril,2005, 84(3):662-671.
[20]嵇文田,田洪志,赵增涛,等.锌硒宝联合左卡尼汀治疗特发性弱精子症120例分析[J].生殖与避孕,2008,28(10)：639,634.
[21]瞿利军.口服西地那非或他达拉非改变不育症男性的精子活力[J].中华男科学杂志,2008,14(1)：92.
[22]Ebisch IM, Thomas CM, Peters WH, et al. The important of folate, zinc and antioxidants in the pathogenesis and prevention of subfertility[J]. Hum Reprod Update,2007,13(2):163-174.
[23]Avdemir B, Onaran I, Kiziler AR, et al. Increased oxidative damage of sperm and seminal plasma in men with idiopathic infertility is higher in patients with glutathione S-transferase Mu-1 null grnotype[J]. Asian J Andral,2007,9(1):108-115.
[24]卢少明,李晓,张浩波,等.克罗米芬联合己酮可可碱治疗特发性少、弱精子症的临床研究[J].中国男科学杂志,2008,22(5)：39-41.
[25]Balercia G, Mosca F, Mantero F, et al. Coenzyme Q(10)supplementation in infertile men with idiopathic asthenozoospermia:an open, uncontrolled pilot study[J]. Fertil Steril,2004,81(1):93-98.
[26]王志强,黄耀全,梁兵.电针与中药治疗男性不育少、弱精子症临床观察[J].中国针灸,2008,28(11)：805-807.
[27]史宏,畅立强,刘美莲,等.左卡尼汀联合加味五子汤治疗男性不育症的经验总结[J].中国性科学,2008,17(11)：28-29.
[28]黄群.自拟生精汤配合促性素治疗少弱精子症56例观察[J].中医药临床杂志,2008,20(3)：272-273.
[2]Check JH. Treatment of male infertility. Clin Exp Obstet Gynecol 2007;34(4):201-206
[3]Dada R, Gupta NP, Kucheria K. Molecular screeningfor Yq microdeletionin men with idiopathic oligozoospermia and azoospermia[J]. J Biosci,2003,28(2):163-168.
[4]Dada R, Gupta NP, Kucheria K. Molecular screening for Yq microdeletionin men with idiopathic oligozoospermia and azoospermia[J]. J Biosci,2003,28(2):163-168.
[5]郭应禄李宏军主编.男性不育症[M].第1版.北京：人民军医出版社,2003.160-166.
[6]世界卫生组织.人类精液及精子—宫颈粘液相互作用实验室检验手册[M].第4版.北京：人民卫生出版社,2001.3-8.
[7]国家技术监督局发布.中华人民共和国国家标准—中医临床诊疗术语证候部分.1997.38-39.
[8]世界卫生组织.不育夫妇标准检查与诊断手册[M].北京：科学出版社,1994.19.
[9]中华人民共和国卫生部.中药新药临床研究指导原则.第1辑[S].1993.192.
[10]樊友平,朱佳卿,李家蓉,等主编,《中华泌尿男科学古典集成》[M].第1版.北京,中医古籍出版社,2007
[11]贾金铭主编.中国中西医结合男科学[M].第1版.北京.中国医药科技出版社,2005,176-178
[12]王琦主编.男科疾病中西医汇通[M].第1版.沈阳：辽宁科学技术出版社,2003.112.
[13]倪良玉.徐福松教授治疗男性不育症经验[J].中医研究,2007,20(12)：43-44.
[14]李日庆.男性不育症中西医治疗概况[J].现代诊断与治疗,2008,19(3)：129-133.
[15]孙自学,陈建设.王琦教授治疗男性不育经验介绍[J].四川中医,2004,22(1)：7-8.
[16]冯德海.郭军教授治疗男性不育症经验总结[J].四川中医,2007,25(6)：7-8.
[17]廖敦,刘志刚.西洋参治疗弱精子症62例疗效观察[J].中国实用医药,2009,5.4(15)：174-175
[18]吴维,刘继红,尹春萍,等.刺五加注射液与茶碱、咖啡因体外对精子运动功能影响的比较研究[J].中华男科学杂志,2009,15(3)：278-281
[19]张燕,郭贤坤,刘汉丹.黄芪注射液体外对精子运动相关参数的影响[J].中药材,2010.7,33(3)：485-486
[20]仇仁良,周惠耕.人参治疗弱精子症不育疗效观察[J].现代中西医结合杂志,2008,4.7(10)：1515
[21]孟庆杰,马啸,郭通航.复方玄驹胶囊联合锌硒宝片对少弱畸形精子症患者精子质量的影响[J].生殖与避孕,2010,7.30(7)：502-503
[22]郭应禄李宏军主编.男性不育症[M].第1版.北京：人民军医出版社,2003.
[23]王维平,汪晶冰,马洪星,等.292例男性少、弱精子症和无精子症患者的细胞遗传学分析[J].中国优生与遗传杂志,2010,18(7)：43
[24]袁慧珍,刘艳秋.男性不育的细胞遗传学分析[J].江西医药,2009,3.44(3)：243-245
[25]陈德宇,刘利敏,谢庆东,等.弱精症患者精子基因表达谱的建立及分析[J].癌变畸变突变,2009,7.21(4)0286-0290
[26]崔艳国,张立新,董丽萍,等.男性低生育人群精浆锌、酸性磷酸酶及血清性激素水平及意义[J].山东医药,2010,50(25)：85-86
[27]宋博,蔡志明.雄激素与精子发生的关系[J].临床泌尿外科杂志,2008，7.23(7)：553-555
[28]郑洁,何彦芳,李俊林.人精子中芳香化酶表达与精子运动能力[J].中国男科学杂志,2009,23(8)：22-24
[29]周其赵,冯春琼,毛向明.弱精子症相关基因与蛋白研究进展[J].中华男科学杂志,2009,15(9)：836-839
[30]Ferlin A, Vinanzi C, Garolla A, et al. Clin Endocrinol(Oxf)2006;65(5):606-610
[31]金龙金,李传连,费前进,等.mtATPase6基因变异与弱精子症的相关分析[J].细胞生物学杂志,2008,30：660-666.
[32]冯春琼,宋艳斌,邹亚光,等.弱精子症患者精子线粒体MTCYB.MTATP6基因的检测[J].中国男科学杂志,2008,14(4)：321-323
[33]李传连,楼哲丰,黄学锋,等.线粒体基因ND3.ND4L核苷酸变异与弱精子症相关分析[J].中国病理生理杂志,2010,26(2)：362-367
[34]Minocherhomji S, Athalye AS, Madon PF, et al. A case-control study identifying chromosomal polymorphic variations as forms of epigenetic alterations associated with the infertility phenotype. Fertil Steril 2009;92(1):88-95
[35]张清健,郑立新,田佩玲,等。精液分析异常患者的染色体分析[J].中国男科学杂志,2010,24(7)：51-53
[36]李练兵,夏彦恺,李新生,等.弱精子症患者精子X,Y，18号染色体的三色荧光原位杂交分析[J].中国男科学杂志,2008,14(3)：211-214.
[37]Appasamy M, Muttukrishna S, Pizzey AR, et al. Relationship between male reproductive hormones, sperm DNA damage and markers of oxidative stress in infertility. Repord Biomed Online 2007;14(2):159-165
[38]Zhang Y, Wang H, Wang LR, et al. The clinical significance of sperm DNA damage detection combined with routin semen testing in assisted reproduction. Molecular Medcine Reports 2008; 1:617-624
[39]孙静波,姜宏,何瑞冰.精子DNA完整性与精液参数的相关性研究[J].中国男科学杂志,2010,24(4)：26-29
[40]Makkerk, Agarwal A, Sharma R. Oxidative stress & male infertility. Indian J Med Res 2009;129(4):357-367
[41]Mehcli M, Khantouche L, Ajina M, et al. Detection of DNA fragmentation in human spermatozoa:correlation with semen parameters. Andrologia 2009;41(6):383-386
[42]Shamsi MB, Venkstesh S, Tanwar M, et al. DNA integrity and semen quality in men with low seminal antioxidant levels. Mutat Res 2009;665(1-2)29-36
[43]Kumar R, Venkatesh S, Kumar M, et al. Oxidative stress and sperm mitochondrial DNA mutation in idiopathic oligoasthenozoospermic men. Indian J Biochem Biophys 2009;46(2):172-177
·-[44]曹兴午,李翠英.线粒体缺失与不育症[J].中国男科学杂志,2010；24(5)：71-72
[45]Rahman MM, Laher I. Structural and functional alteration of blood vessels caused by cigarette smoking:an overview of molecular mechanisms. Curr Vase. Pharmacol 2007;5(4):276-292
[46]Shamsi MB, Venkstesh S, Tanwar M, et al. DNA integrity and semen quality in men with low seminal antioxidant levels. Mutat Res 2009;665(1-2)29-36
[47]赵洪鑫,史庭燕,时伟丽,等.应用化学发光法检测男性不育人群精液活性氧水平[J].中国男科学杂志,2009,23(4)：14
[48]刘居理,罗明,卢雪芳,等.少弱精子症、弱精子症患者精浆活性氧浓度检测及其应用价值.中国实验诊断学,2009,9,13(9)：1275-1276
[49]罗胜萍,吴蓉,刘自刚.男性不育患者精浆锌含量的变化分析[J].中国实验诊断学,2009,6.13(6)：828-829
[50]张睿.男性少弱精子症患者精浆锌含量分析[J].临床研究,2010,5.48(15)：29-30
[51]Espino J, Mediero M, Lozano GM, et al. Reduced levels of intracellular calcium releasing in spermatozoa from asthenozoospermic patients. Reprod Biol Endocrinol 2009;7:11
[52]薛炜,邢俊平.弱精子症患者与正常精子人群精子相比多不饱和脂肪酸减少,饱和脂肪酸含量增加[J].现代泌尿外科杂志,2008,13(1)：
[53]杨晓华,耿翠艳.少、弱、畸形精子症患者血铅、镉浓度的测定[J].中国男科学杂志,2008,22(9)：53-55.
[54]张宁,汤洁,鲁海鸥,等。低强度微波辐射和低频噪声对男性精子影响的流行病学调查研究[J]。中国男科学杂志,2007,21(6)：23-25,29
[55]Jacosson B, Aaltonen R, Rantakokko-Jalava K, et al. Quantification of Ureaplasma urealyticum DNA in the amniotic fluid from patients in PTL and PPROM and its relation to inflammatory cytokine levels. Acta Obstet Gynecol Scand 2009;88(1):63-70
[56]徐峥,白云,谭巨莲,等.解脲支原体感染患者精浆中细胞因子对精子活力的影响[J].中国男科学杂志,2010,24(5)：30-32
[57]邵欣胤,李金彪,朱琳.体外精浆置换对弱精子症患者精子运动能力的影响[J].山东医药,2010,50(6)：51-52
[58]郭应禄李宏军主编.男性不育症[M].第1版.北京：人民军医出版社,2003.226-229
[59]Balercia G, Regoli F, Armeni T, et al.. Placebo-controlled double-blind randomized trial on the use of L-carnitine, L-acetylcarnitine, or combined L-carnitine and L-acetylcarnitine in men with idiopathic asthenozoospermia[J]. Fertil Steril,2005, 84(3):662-671.
[60]Ebisch IM, Thomas CM, Peters WH, et al. The important of folate, zinc and antioxidants in the pathogenesis and prevention of subfertility[J]. Hum Reprod Update, 2007,13(2):163-174.
[61]Balercia G, Mosca F, Mantero F, et al. Coenzyme Q(10)supplementation in infertile men with idiopathic asthenozoospermia:an open, uncontrolled pilot study[J]. Fertil Steril,2004,81(1):93-98.
[62]瞿利军.口服西地那非或他达拉非改变不育症男性的精子活力[J].中华男科学杂志,2008,14(1)：92.
[63]卢少明,李晓,张浩波,等.克罗米芬联合己酮可可碱治疗特发性少、弱精子症的临床研究[J].中国男科学杂志,2008,22(5)：39-41.
[64]阮衍泰.它莫西芬配合金施尔康、肾肾气丸对精液质量的改善[J].现代泌尿外科杂志,2009,1.14(1)：32-34
[65]嵇文田,田洪志,赵增涛,等.锌硒宝联合左卡尼汀治疗特发性弱精子症120例分析[J].生殖与避孕,2008,28(10)：639,634.
[66]张利,段晓明,徐计秀,等.左旋肉碱加五子衍宗丸治疗少弱精子症[J].中国中西医结合外科杂志,2010,10.16(5)：535-537
[67]卜律花.多维元素配合IMC治疗少精弱精症的疗效观察[J].中国计划生育学杂志,2009，7.165：434-435
[68]徐波,付伟.弱精子症的中医证治研究[J].中国当代医药,2009,3.16(5)：58-60
[69]申树林,赵倩,徐刚,等.养精汤治疗特发性弱精子症的疗效观察[J].中华男科学杂志,2010,16(6)：569-57
[70]Ladachart Taepongsorat, Prakong Tangpraprutgul, Noppadon Kitana, et al. Stimulating effects of quercetin on sperm quality and reproductive organs in adult male rats. Asian J Androl 2008;10(2):249-258
[71]钟百灵.愈精颗粒治疗男性不育症药效学研究[J].山东中医杂志,2002,21(3)：177-179.
[72]颜正华主编.中药学[M].第2版.北京.人民卫生出版社.2006.
[73]王本祥主编.现代中药药理与临床[M].第1版.天津.天津科技翻译出版公司,2004.
[1]王琦主编.男科疾病中西医汇通[M].第1版.沈阳：辽宁科学技术出版社,2003.112.
[2]杨建华主编.实用男性不育诊疗学[M].第1版.上海：第二军医大学出版社,2002.178-180.
[3]郭雪华,郑连文.弱精子症的研究进展[J].中国男科学杂志,2008,(22)6：54-56.
[4]金龙金,李传连,费前进,等.mtATPase6基因变异与弱精子症的相关分析[J].细胞生物学杂志,2008,30：660-666.
[5]冯春琼,宋艳斌,邹亚光,等.弱精子症患者精子线粒体MTCYB、MTATP6基因的检测[J].中国男科学杂志,2008,14(4)：321-323.
[6]李练兵,夏彦恺,李新生,等.弱精子症患者精子X,Y，18号染色体的三色荧光原位杂交分析[J].中国男科学杂志,2008,14(3)：211-214.
[7]薛炜,邢俊平.弱精子症患者与正常精子人群精子相比多不饱和脂肪酸减少,饱和脂肪酸含量增加[J].现代泌尿外科杂志,2008,13(1)：
[8]杨晓华,耿翠艳.少、弱、畸形精子症患者血铅、镉浓度的测定[J].中国男科学杂志,2008,22(9)：53-55.
[9]孙自学,陈建设.王琦教授治疗男性不育经验介绍[J].四川中医,2004,22(1)：7-8.
[10]李曰庆.男性不育症中西医治疗概况[J].现代诊断与治疗,2008,19(3)：129-133.
[11]倪良玉.徐福松教授治疗男性不育症经验[J].中医研究,2007,20(12)：43-44.
[12]冯德海.郭军教授治疗男性不育症经验总结[J].四川中医,2007,25(6)：7-8.
[13]胡廉,熊承良.野山楂根含药血清对弱精子症患者精子运动参数影响的体外研究[J].中国中药杂志,2006,31(4)：333-335.
[14]李忠远,陈智.刺五加水提物对人精子体外运动活力的影响[J].医药导报,2006,25(5)：387-389.
[15]卢太坤.虎杖丹参饮治疗免疫性不育男性的临床研究[J].中华男科学杂志,2006,12(8)：750-755.
[16]张俊慧,王楠,曹爽,等.枸杞子在男性不育症中的应用[[J].中华男科学杂志,2008,14(3)：279-281.
[17]郭应禄李宏军主编.男性不育症[M].第1版.北京：人民军医出版社,2003.160-166.
[18]程怀瑾,陈廷.左旋肉碱和乙酰左旋肉碱复合制剂对特发性弱精子症精子质量的影响[J].中华男科学杂志,2008,14(2)：149-151.
[19]Balercia G, Regoli F, Armeni T, et al.. Placebo-controlled double-blind randomized trial on the use of L-carnitine, L-acetylcarnitine, or combined L-carnitine and L-acetylcarnitine in men with idiopathic asthenozoospermia[J]. Fertil Steril,2005, 84(3):662-671.
[20]嵇文田,田洪志,赵增涛,等.锌硒宝联合左卡尼汀治疗特发性弱精子症120例分析[J].生殖与避孕,2008,28(10)：639,634.
[21]瞿利军.口服西地那非或他达拉非改变不育症男性的精子活力[J].中华男科学杂志,2008,14(1)：92.
[22]Ebisch IM, Thomas CM, Peters WH, et al. The important of folate, zinc and antioxidants in the pathogenesis and prevention of subfertility[J]. Hum Reprod Update,2007,13(2):163-174.
[23]Avdemir B, Onaran I, Kiziler AR, et al. Increased oxidative damage of sperm and seminal plasma in men with idiopathic infertility is higher in patients with glutathione S-transferase Mu-1 null grnotype[J]. Asian J Andral,2007,9(1):108-115.
[24]卢少明,李晓,张浩波,等.克罗米芬联合己酮可可碱治疗特发性少、弱精子症的临床研究[J].中国男科学杂志,2008,22(5)：39-41.
[25]Balercia G, Mosca F, Mantero F, et al. Coenzyme Q(10)supplementation in infertile men with idiopathic asthenozoospermia:an open, uncontrolled pilot study[J]. Fertil Steril,2004,81(1):93-98.
[26]王志强,黄耀全,梁兵.电针与中药治疗男性不育少、弱精子症临床观察[J].中国针灸,2008,28(11)：805-807.
[27]史宏,畅立强,刘美莲,等.左卡尼汀联合加味五子汤治疗男性不育症的经验总结[J].中国性科学,2008,17(11)：28-29.
[28]黄群.自拟生精汤配合促性素治疗少弱精子症56例观察[J].中医药临床杂志,2008,20(3)：272-273.