不同产地冬枣对比试验及冬枣标准化栽培研究
详细信息    本馆镜像全文|  推荐本文 |  |   获取CNKI官网全文
摘要
冬枣(Zizyphus jujuba Mill. cv. Dongzao)是我国特有的优良晚熟、鲜食枣品种。在冬枣产业迅速发展的过程中,出现不同产地争当“第一”、盲目引种、果实品质下降等问题。本研究针对以上问题,在山东沾化、河北黄骅和沧县三地设立产地对比试验园,将选自不同产地(沾化、黄骅、庆云、沧县和乐陵)的冬枣在同一立地条件下栽培,对冬枣果实和果核表型、果实营养组成、感官评价、抗寒性和光合生理指标等进行了连续4年的测定分析,探讨了冬枣产生差异及品质下降的真正原因;本文还进行了冬枣cDNA文库构建、测序和生物信息学分析;研究了冬枣主要病虫害的无公害防治措施并探索了冬枣杂交育种技术。主要研究成果如下:
     1.多点、多年观测结果表明,在同一年份、同一试验园内,不同产地冬枣在果实和果核表型、果实营养组成和感官评价等方面没有明显差异;而在不同年份、不同试验园之间,冬枣果实表型和品质各项指标差异显著。说明影响冬枣果实表型和品质的主要因素是环境条件和栽培管理措施,而不是产地来源。
     就冬枣群体而言,在长期栽培过程中,由于芽变和人工选择,仍存在着个别单株的差异。所以,冬枣改良的方向不是产地选择,而是单株选择。
     2.冬枣叶片N/P变化范围为11.11~13.54,属于N含量制约的植物类型。冬枣叶片N、P、K元素含量在7月~9月初比较稳定,这段时间是上述3种元素最佳营养诊断采样期。
     3.冬枣的光合性状受环境的影响变化较大,测定结果与测定时间、地点甚至部位等均有很大关系。沧州地区不同产地冬枣叶片Pn的日变化呈较为明显的不对称双峰曲线,年变化呈单峰曲线,冬枣为中午光合速率降低型植物。
     4.温度的骤变是可能引起冬枣冻害的一个重要原因,应当根据各地实际情况注意防护;冬枣在适生区栽植也要防止个别年份出现极端低温时遭受冻害;温度条件不适合的地区不可盲目引种,进行栽培时应采取保护措施。5个不同产地冬枣抗寒力有一定差别,但在不同指标间表现不一致。不同产地冬枣在沧州地区均能正常生长,无冻害发生。
     5.利用3种不同发育时期的冬枣果实构建混合cDNA文库,从该cDNA文库中随机挑取单克隆进行5’端单向测序,得到1060条EST序列,经过cross-match和CAP3软件处理后共获得628条Unigene。在Uniprot数据库中比较发现有534条Unigene与已知基因具有同源序列,占Unigene总数的85%。利用ESTPiper在线分析软件对Unigene进行GO功能基因分类,发现273条可以在“分子功能”、“生物学过程”、“细胞内组分”得到分类。鉴定出MYB转录因子、肉桂醇脱氢酶、甘露糖转运等与果实品质形成相关功能基因以及ACC氧化酶、超氧化物歧化酶、热激蛋白等与冬枣果实后熟相关功能基因。利用PERL程序对628条Unigene进行EST-SSR分析,结果表明在116条EST序列中包含有147个SSR,以二核苷酸和三核苷酸为主要重复类型。
     6.在调查已有栽培技术和自己试验的基础上,本研究总结课题组多年工作,结合生产实践,制定了《绿色食品(AA级)冬枣栽培技术规程(草案)》,为实施冬枣无公害栽培提供了依据和工作细则。
     7.冬枣人工杂交育种困难,本研究中杂交试验失败的主要原因是冬枣坐果率低、种仁率低、胚败育,而且杂交时授粉时期晚、部分品种花期不遇,并遭遇绿盲蝽象危害等,应开拓枣树育种的新思路。
Dongzao jujube(Zizyphus jujuba Mill. cv. Dongzao) is regarded as the best late maturing and fresh cultivars of Chinese jujubes. With the rapid development of Dongzao jujube's industry in recent years, such problems as the degradation of fruit quality, trying to be first, blind introduction happened. Three comparison orchards, which located at Zhanhua county in Shandong province, Huanghua and Cangxian county in Hebei province were established in order to probe the cause of fruit degradation and variation of quality. Such indexes as the fruit quality, the fruit stone phenotype, components of fruit nutrients, food sensory evaluation, cold hardiness and photosynthetic physiology were analyzed at these three comparison orchards for 4 years.
     The paper also carried a jujube cDNA library construction, sequencing and bioinformatics analysis; pollution-free prevention and the feasibility of cross breeding of Dongzao jujube were also studied in this work. Following was the key results in this thesis.
     1. There was no qualitative difference for Dongzao jujubes of different provenances in the same comparison orchard and in the same year. But the difference of Dongzao jujube between different orchards and different years was significant. This results showed that the phenotypic and fruit quality of Dongzao jujube were mainly contributed by environmental conditions, cultivation and management measures, but not by provenances. Because the long-term effect of mutant and artificial selection, there are still variations for individual plant. The direction of genetic improvement was individual selection not group selection.
     2. The N/P of the leaf of Dongzao jujube ranges from 11.11 to 13.54, which indicated Dongzao jujube was a kind of vegetation type influenced by the content of N. The contents of N, P, K in Dongzao jujube leaf were stable from July to September, which was the optimal sampling period for nutrition diagnostic.
     3. Photosynthetic characteristics of Dongzao jujube were greatly influenced by the environment varation, which were different with the sampling time, place and sampling part. The diurnal variation of Pn in Cangzhou region showed obvious asymmetry double curve, while annual change showed a single peak curve, Dongzao jujube was decline photosynthetic rates plant at noon.
     4. The sudden change of air temperature is an important factor which leads to freezing of Dongzao jujube, so the protection should be based on the actual situation in different areas. Freezing damage which was caused by extreme low temperature even should be to prevented in Dongzao jujube normal region. Blind introduction should be avoided, especially for those places which temperature conditions are not suitable for the cultivation of Dongzao jujube. Some protective measures were necessary for cultivation. There was some difference between different provenances of Dongzao jujube, but no trees suffered from freezing injury under natural situation in Cang County, Hebei Proviance of China.
     5. The fruit of three different development stages were equally mixed to construct a fruit cDNA library.1060 EST sequences were obtained by one run sequencing from 5' end which was randomly picked from cDNA library.628 Unigenes were obtained after analyzed by cross-matching and CAP3 software. Compared with Uniprot database,534 Unigene sequences were identified with known homologous genes, accounting for 85% of the total number of Unigene. A GO analysis was carried out by ESTPiper, an online software, which resulted that 273 Unigene sequences could be classified in the "molecular function", "biological process" and "cellular component". Some fruit quality related genes, such as MYB transcription factor, Cinnamyl alcohol dehydrogenase, GDP-mannose transporter, and some genes associated with jujube fruit ripening genes, such as the ACC oxidase, superoxide dismutase, heat shock protein, also could be identified. Furthermore, the Unigene sequences were used for EST-SSR analysis, which revealed 147 SSRs in 116 EST sequences. The dinucleotide and trinucleotide repeats were found as the main motif types.
     6. Based on pollution-free pest control and pollution-free cultivation, "Technical specification for the fruit production of Ziziphus jujube cv. Dongzao at green food AA grade " was compiled, which provides a theoretical basis and working instruction for the pollution-free cultivation of Dongzao jujube.
     7. There were difficulties in artificial crossbreeding for Dongzao jujube. The main reasons for failure of crossbreeding were asynchronous flowering of the related cultivars, delaying pollination, low fruit-setting rate, very low kernel percentage, embryo abortion, and damage induced by Lygus bug and so on.
引文
1.毕平,康振英,来发茂.观赏枣品种介绍[J].山西果树,1995,(2):20;
    2.曹慧娟.植物学(第2版)[M].北京:中国林业出版社,1992;
    3.曹军胜,刘广全.刺槐光合特性的研究[J].西北农业学报,2005,14(3):118-122,136;
    4.陈弟,符贤英,殷晓敏,等.几种果实总RNA提取方法的评价[J],广东农业科学,2007,11:30-33;
    5.陈建华,曹阳,李育忠,等.不同板栗品种的矿质元素含量与开花结实的关系[J].经济林研究,2005,23(2):1-4:
    6.陈精玉,刘成强,张林.冬枣丰产管理技术[J].果农之友,2005(2):33-34;
    7.陈晓阳,沈熙环.林木育种学[M].北京:高等教育出版社,2005;
    8.陈学森,陈学森,张艳敏.杏及大樱桃花器官冻害调查[J].园艺学报,2001,28(4):373;
    9.陈学森,李宪利,张艳敏,等.杏种质资源评价及遗传育种研究进展[J].果树学报,2001,18(3):178-181:
    10.陈学森,杨红花,刘焕芳,等.利用远缘杂交创造核果类果树新种质的研究[J].落叶果树,2004,(6):4-6:
    11.陈艳秋,曲柏宏,牛广才,等.苹果梨果实矿质元素含量季节变化规律[J].延边大学农学学报,2000,22(2):109-111:
    12.陈艳秋,曲柏宏,牛广才,等.苹果梨果实矿质元素含量及其品质效应的研究[J].吉林农业科学,2000,25(6):44-48;
    13.陈竹君,周建斌,史清华,等.猕猴桃叶内矿质元素含量年生长季内的变化[J].西北农业大学学报,1999,27(5):54-57;
    14.储昭晖,彭开蔓,张利达,等.水稻全生育期均一化cDNA文库的构建和鉴定[J].科学通报,2002,47(21):1656-1662:
    15.崔柏瑄,韩有志,李乐.不同大果沙棘品种光合生理特征测定[J].国际沙棘研究与开发,2009,6(4): 12-17:
    16.邓令毅,王洪春.葡萄的抗寒性与质膜透性[J].植物生理学通讯,1984,(2):12-16;
    17.邓秀新,Grosser JW, Gmitter FGJR,等.柑桔种间体配融合及培养研究[J].遗传学报,1995,22(4):316-321:
    18.丁勤.油桃(Prunus persica var.nectarina Maxim)果实生长发育与裂果关系的研究[D].西安:西北农林科技大学,2004;
    19.丁松爽,苏培玺,严巧娣.不同间作条件下枣树的光合特性研究[J].干旱地区农业研究,2009,27(1):184-189;
    20.董清华,冯永庆,秦岭,等.葡萄果实cDNA文库的构建及鉴定[J].分子植物育种,2007,5(1):117-120;
    21.段晓明,殷卫.植物源农药的研究进展[J].青海大学学报(自然科学版),2006,24(5):56-60;
    22.樊卫国,向显衡,王廷强,等.刺梨果实发育的研究[J].山地农业生物学报,1992,11(1):74-79;
    23.冯学文.果肉硬溶质水蜜桃杂交育种[J].河北果树,2000,(2):14-15;
    24.付昌斌.砂地柏果实提取物对棉铃虫生长发育的影响[J].西北农业大学学报,1998,26(1):9;
    25.高京草,王长柱,高文海.从2007年枣大面积裂果谈我国枣枣产业调整[J].中国果树,2008,(4):64-65:
    26.高启明,李疆,张传来,等.金光杏梅果实生长发育期间几种矿质元素含量的变化[J].果树学报,2005,22(4):331-334;
    27.高琼.胡枝子不同种和种源耐旱、耐寒性变异研究[D].北京:北京林业大学,2005;
    28.高煜珠,王忠.关于光呼吸与光合作用关系的研究[J].植物生理学报,1982,8(4):373-384;
    29.耿雪青.苹果果实cDNA文库的构建[D].北京:中国农业大学,2003;
    30.龚云池,徐季娥.鸭梨叶片和果实中钙素含量年周期变化的研究[J].园艺学报,1987,26(4):1-5:
    31.龚云池,徐季娥,吕瑞江.梨果实中不同形态钙的含量及其变化的研究[J].园艺学报,1992,19(2):129-134;
    32.顾曼如,束怀瑞.红星苹果果实矿质元素含量与品质的关系[J].园艺学报,1992,19(4):301-306;
    33.管东生,罗琳.海南热带植物叶片化学元素含量特征[J].林业科学,2003,39(2):28-32;
    34.郭传友,黄坚钦,王正加,等.大别山山核桃果实品质与土壤性质的相关分析[J].经济林研
    究,2006,24(4):19-22;
    35.郭红丽.我国30种水果维生素及矿质元素的综合评价[J].微量元素与健康研究,2009,26(4):35-36:
    36.郭裕新,单公华,杨茂林.我国枣树的区化栽培[J].中国果树,2002,4:44-46,51;
    37.郭裕新.目前鲁北冬枣生产存在的问题和解决途径[J].落叶果树,2005(5):1-5;
    38.韩斌.枣果实总RNA的提取及其反转录技术体系的建立[D].保定:河北农业大学,2006;
    39.郝丽娟,邵青玲.不同杏品种的抗寒性研究[J].山西果树,2006,(5):3-5;
    40.何军,马志卿,张兴.植物源农药概述[J].西北农林科技大学学报(自然科学版),2006,34(9):79-85:
    41.胡建斌,李建吾.甜瓜EST-SSR位点信息及标记开发[J].园艺学报,2009,36(4):513-520;
    42.胡莽,贺超.试论社区参与在三北防护林建设中的作用[J].防护林科技,2003,(3):95;
    43.胡淑静,宫荣宽.冬枣的丰产栽培[J].安徽科技,2006(7):30-31;
    44.胡新艳.产地和肥料对冬枣主要性状表现的影响[D].北京:北京林业大学,2005;
    45.郇树峰.探析我国鲜枣出口的制约因素及其对策[J].中国果菜,2001(5):10;
    46.黄秀花,王保侠,张莉,等.鲁北冬枣引至江苏省铜山县的表现[J].落叶果树,2006,38(2):37;
    47.黄旭明,袁炜群,王惠聪,等.抗裂性不同的荔枝品种果皮发育过程中钙的分步动态研究[J].园艺学报,2005,32(4):578-583;
    48.黄永红,沈洪波,陈学森.杏树抗寒生理研究初报[J].山东农业大学学报(自然科学版),2005,36(2):191-195:
    49.简令成.生物膜与植物抗寒害和抗寒性的关系[J].植物学通报,1983,(1):17-23;
    50.简令成.植物抗寒性的细胞及分子生物学研究进展[J].细胞生物学进展,1990,(2):296-320;
    51.江东,钟广炎,洪棋斌.柑橘EST-SSR分子标记分析[J].遗传学报,2006,33:345-353;
    52.江绍玫.植物源无公害农药研究开发现状[J].江西农业大学学报,2000,22(1):140-142;
    53.金基强,崔海瑞,龚晓春,等.用EST-SSR标记对茶树种质资源的研究[J].2007,29(1): 103-108;
    54.雎薇,丁晓东,霍俊伟,等.草原樱桃与欧洲甜樱桃远缘杂交不亲和原因初探[J].东北农业大学学报,1999,30(1):148-153;
    55.康国栋,,程存刚,李敏,等.水分胁迫对苹果不同品种光合特性的影响[J].吉林农业大学学报,2008,30(1):31-35;
    56.李保国,王永蕙.增施氮肥和环剥对枣树光合速率的影响[J].河北农业大学学报,1991,143:33-37;
    57.李保国,徐爱春,齐国辉,等.红富士苹果叶片主要矿质元素含量变化规律研究[J].河北林果研究,2006,21(3):296-299;
    58.李晨,阎晓红,周新安,等.大豆种子不同发育时期全长均一化cDNA文库的构建[J].中国农业科学,2010,43(3):462-467;
    59.李发东,张万军,杨永辉等.太行山果树引种与生态适应性研究[J].生态经济,2002(8):57-59;
    60.李锋,张凤芬,曹希俊,等.李、杏及杂种间远缘杂交亲和性研究[J].吉林农业大学学报,1995,17(4):36-39:
    61.李海权.梨果实cDNA文库的构建[D].保定:河北农业大学,2004;
    62.李宏,王新力.植物组织RNA提取的难点及对策[J].生物技术通报,1999,1:36-39;
    63.李建国,黄辉白.荔枝果实理化特性及果皮形态学与裂果易感性的关系[J].华南农业大学学报,1995,16(1):84-89;
    64.李建国,黄辉白,黄旭明.环切对糯米糍果实大小和裂果的影响[J].果树学报,2004,21(4):379-381;
    65.李建明,李洪杰,柴守诚,等.普通小麦Genomic-SSR和EST-SSR分子标记遗传差异[J].安徽农业科学,2007,35(26):8173-8175,8183;
    66.李丽峰.冬枣病虫害无公害综合防治技术[J].中国果业信息,2005(6):49-50;
    67.李利红,任敏.杏树花芽分化期叶片矿质元素含量动态变化的研究[J].安徽农业科学,2007,35(21):6361-6362:
    68.李林光,邹显昌,王金正.油桃果实发育规律和叶片营养特性研究[J].落叶果树,1997,增刊:38-39;
    69.李守勇.不同产地冬枣品质特性及其遗传变异研究[D].北京:北京林业大学,2004;
    70.李小白,张明龙,崔海瑞.油菜EST-SSR标记的建立[J].分子细胞生物学报,2007,40(2):137-144;
    71.李小白,张明龙,崔海瑞.油菜EST-SSR资源的SSR信息分析[J].中国油料作物学报,2007,29(1):20-25:
    72.李秀霞,齐曼·尤努斯,高桥久光,等.Na2SO4胁迫对沙枣光合速率及其它生理指标的影响[J].新疆农业科学,2005,42(2):102-106;
    73.李颖岳.引进台湾青枣品种设施栽培的对比研究[D].北京:北京林业大学,2005;
    74.李永强,李宏伟,高丽锋,等.基于表达序列标签的微卫星标记(EST-SSR)研究进展[J].植物遗传资源学报,2004,5:91-95;
    75.李志扬.枣树优良品种简介及矮密栽培技术要点[J].陕西林业科技,1996,3:68-71;
    76.梁志宏,陈建军,王玉安.梨枣与中国冬枣引种观察结果初报[J].甘肃农业科技,2003(8): 34-35;
    77.林绍艳.海7124纤维/胚珠cDNA文库的构建、测序及EST分析[D],南京:南京农业大学,2008;
    78.刘春杰.沾化冬枣无公害栽培技术[J].农业科技通讯,2006(2):50;
    79.刘多红.二三四团沾化冬枣引种示范[J].新疆农垦科技,2005(2):21;
    80.刘桂丰,褚延广,王玉成,等.星星草cDNA文库构建和金属硫蛋白(MT-1)基因的克隆[J].植物生理学通讯,2005,41(4):424-428;
    81.刘汉云,李铭,张步青,等.枣不同品种光合特征及叶片解剖结构的研究[J].经济林研究,2009,27(2):10-15:
    82.刘金莲.无公害冬枣栽培技术[J].农业环境与发展,2004(3):15;
    83.刘京涛,刘合昌,徐兴学等.冬枣无公害高产优质生产技术[J].中国果菜,2006(6):12-13;
    84.刘孟军.中国枣产业发展报告[M].北京:中国林业出版社,2008;
    85.刘星,仇雪梅,高祥刚,等.基因文库构建及其在鱼类遗传研究上的应用[J].现代农业科技,2008,(16):247-249:
    86.刘勇,刘善军,霍光华,等.甜柿果实发育期间矿质元素和营养成分变化[J].江西农业大学学,2000,22(2):265-270;
    87.卢春生,雷茵霞,张新华.无核白葡萄果实发育期间矿质元素和营养成分变化[J].西北农业学报,1999,8(1):91-94;
    88.路得·布尔班克.如何培育植物为人类服务[M].孟光裕译.北京:科学出版社,1962;
    89.罗都强,冯俊涛,胡瓒,等.雷公藤总生物碱分离及杀虫活性研究[J].西北农林科技大学学报(自然科学版),2001,29(2):61-64;
    90.罗志勇,刘水平,陆秋恒,等.人参植物与皂苷生物合成相关的差减cDNA文库构建及基因差异表达分析[J].生命科学研究,2003,71(4):324-328;
    91.骆蒙,龙秀英,贾继增.几种cDNA差减文库构建方法的比较[J].生物技术通报,2000,(6):14-17:
    92.马恩凤.当前枣树管理中存在的问题及对策[J].天津农林科技,2000,10(5):31-32;
    93.马福广.冬枣在大连地区的表现[J].北方果树,2000,(2):12-13;
    94.马贯羊,张淑英,尹华.核桃属种间远缘杂交育种初报[J].河南林业科技,2009,29(1):13-14;
    95.马建军.山楂花序矿质元素的含量及其动态变化[J].河北职业技术师范学院学报,1999,13(3):29-33:
    96.马庆华,毛永民,申连英,等.枣不同品种花粉生活力和贮藏方法研究[J].干果研究进展,2003(3):46-49:
    97.马庆华.冬枣实生后代主要性状遗传变异规律及枣实生苗抗寒性研究[D].保定:河北农业大学,2004;
    98.马庆华,毛永民,申连英等.枣不同品种果核性状研究[J].河北农业大学学报,2006(1):41-45:
    99.马庆华.不同产地冬枣遗传品质差异及其栽培技术研究[D].北京:北京林业大学,2007;
    100.马瑞娟,杜平,胡金良,等.桃、李种间远缘杂交亲和性研究[J].果树学报,2005,22(3):283-285;
    101.毛永民,申莲英,王永蕙,等.不同树势婆枣叶片矿质元素含量及其年周期变化的研究[J].河北农业大学学报,1991,14(4):15-18;
    102.孟玉平,曹秋芬.枣裂果的原因及防治技术[J].山西果树,2007,(2):15-17;
    103.宁婵娟,吴国良.梨树体内矿质元素分布变化规律[J].山西农业科学,2009,37(7):37-39;
    104.牛治宇,茶正早,何鹏.海南两种芒果树的叶片营养规律[J].热带农业科学,2002,22(4):16-21;
    105.彭建营,梁春莉,张云鹏,等.3个主栽枣品种花粉贮藏及其发芽特性的研究[J].北方植物学研究,2002,4:38-41;
    106.彭建营,束怀瑞,彭士琪,等.用RAPD技术探讨中国枣的种下区划[J].植物分类学报,2002,40(1):89-94;
    107.彭士琪,续九如,刘孟军等.枣树发展中的若干问题和对策[J].干果研究进展,2003,(3):6-8;
    108.祁业凤.枣胚败育机理与胚培养研究[D].保定:河北农业大学,2002;
    109.齐建勋,王克建,吴春林,等.核桃EST-SSR标记的开发[J].农业生物技术学报2009,17(5):872-876;
    110.曲宪忠.无公害农产品生产技术[M].石家庄:河北科学技术出版社,2004;
    111.曲泽洲,王永蕙.中国果树志·枣卷[M].北京:中国林业出版社,1993;
    112.申莲英.枣遗传图谱构建及性状的QTL定位研究[D].保定:河北农业大学,2005;
    113.沈德绪.果树育种学(第2版)[M].北京:中国农业出版社,1995;
    114.沈洪波,陈学森,张艳敏.果树抗寒性的遗传与育种研究进展[J].果树学报,2002,19(5):292-297;
    115.沈洪波.杏品种抗寒性研究[D].泰安:山东农业大学,2002;
    116.沈建国,翟梅枝,林奇英,等.我国植物源农药研究进展[J].福建农林大学学报(自然科学版),2002,31(1):26-31;
    117.沈寅初,张一宾.生物农药[M].北京:化学工业出版社,2000;
    118.宋宏伟,卢绍辉,刘俊磊.几种生物杀虫剂防治枣尺蠖试验[J].农药,2006,45(4):278-279;
    119.宋艳波,杨佩芳,李六林,等.枣树年周期中叶片矿质元素含量动态变化及其相关性研究[J].山西农业大学学报,2006,26(4):340-341;
    120.苏满意,朱正阳.黄骅冬枣引种试验初报[J].河北林业科技,2006(1):12;
    121.苏少堂,董效伦,郭丽丽等.冬枣无公害管理措施[J].林业实用技术,2006(8):34;
    122.孙秉钧,黄礼森,李树玲,等.利用电介质渗出率方法测定梨的耐寒性[J].中国果树,1987(1): 15-18:
    123.孙灿辉.鲁北冬枣引种栽培技术[J].安徽林业,2003(3):12;
    124.孙霞,柴仲平,蒋平安.水、氮对南疆‘红富士’苹果光合特性日变化的影响[J].北方果树,2010(1):3-6;
    125.孙雪.龙须菜cDNA文库构建及EST和SSR分析[D].青岛:中国海洋大学,2003;
    126.唐凤德,蔡天革.化控物质对紫椴苗木抗寒生理特性的影响[J].辽宁大学学报(自然科学版),2004,31(4):355-357;
    127.唐英,谭世语,孙大贵,等.我国植物源农药的研究进展[J].西南民族大学学报·自然科学版,2003,29(3):363-366;
    128.田伟政,陈志阳.湘南地区冬枣栽植成活率比较试验[J].中国南方果树,2005,34(1):56;
    129.王继和,马全林,吴春荣,等.干旱沙区几种果树生理生态特性及其适应性研究[J].中国沙漠,2001,21(增刊):22-29;
    130.王嘉玲.梨枣引种试栽初报[J].辽宁林业科技,1996,3:45-46;
    131.王晶,曹兵,张光弟.不同施氮水平下盐胁迫对灵武长枣苗木光合特性的影响[J].北方园艺,2009(1):34-36:
    132.王玖瑞.枣树雄性不育和胚败育研究[D].保定:河北农业大学,2004;
    133.王秋晓,韩明玉,赵彩平.桃不同树形光合特性的研究[J].2009,37(4):145-150;
    134.王荣富.植物抗寒指标的种类及其应用[J].植物生理学通讯,1987,(3):49-55;
    135.王圣梅,黄仁煌,武显维,等.猕猴桃远缘杂交育种研究[J].果树科学,1994,11(1):23-26;
    136.王世威,薛军,续九如.北京地区冬枣引种试验初报[J].北京林业科技,1992,(1):19-22;
    137.王同坤,马建军,朱京涛.山楂果实矿质营养元素含量的年周期变化规律[J].园艺学报,1994,21(2):201-202;
    138.王中英.我国新育成的优良抗寒果树品种资源[J].世界农业,1996,6:30-33;
    139.伍维模,董合林,危常洲,等.南疆露地棉与海岛棉光和-光响应及叶绿素荧光特性分析[J].西北农业学报,2006,15(4):141-146;
    140.武婷,武之新.冬枣施肥技术研究进展[J].西北园艺,2009,(6):7-8;
    141.武维华.植物生理学[M].北京:科学出版社,2003;
    142.武之新.冬枣周年管理技术-冬枣施肥的种类和施肥方法[J].农家顾问,2008,(4):28-30;
    143.武之新.冬枣周年管理技术-冬枣追肥新技术[J].农家顾问,2008,(5):28-29;
    144.郗荣庭,胡庆祥,张玉星,等.鸭梨果实氮和矿质元素含量变化及其相关性[J].园艺学报,1997,24(3):285-286:
    145.夏生林.北方冬枣落户江南栽培技术[J].落叶果树,2004,20(4):38;
    146.谢文贵,陈家龙,周健红.大红甜橙铁素含量与其他矿质元素的相关性[J].贵州农学院学报,1997,16(1):58-61;
    147.忻雅,崔海瑞,卢美贞,等,白菜EST-SSR信息分析与标记的建立[J].园艺学报,2006,33(3):549-554:
    148.忻雅,崔海瑞,张明龙,等,白菜EST-SSR标记的通用性[J].细胞生物学杂志,2006,28:248-252;
    149.熊燕,张万民.中国野生葡萄抗寒研究利用[J].安徽农业科学,2007,35(11):3238-3239;
    150.徐光泉.冬枣保花促果技术简介[J].安徽林业,2005(3):46;
    151.徐汉虹.八角首香精油的杀虫活性与化学成分研究[J].植物保护学报,1996,23(3):338;
    152.徐康乐,刘哲宁,杜磊等.鲁北冬枣的主要病虫害[J].落叶果树,2005(3):45-46;
    153.徐立新.冬枣花果管理技术[J].河北果树,2006(4):54-55;
    154.徐效俊.沼肥在冬枣生产中的应用研究[J].可再生能源,2005,(5):66-67;
    155.许大全.光合速率、光合效率与作物产量[J].生物学通报,1999,34(8):8-10;
    156.续九如,李守勇,张昌盛.冬枣的形态特征及其与其他晚熟枣品种的区别[J].河北林果研究,2003,3:38-42:
    157.严金娥.冬枣无公害丰产优质栽培技术[J].河北果树,2006(6):34;
    158.晏慧君,黄兴奇,程在全.cDNA文库构建策略及其分析研究进展[J].云南农业大学学报, 2006,21(1):1-6:
    159.杨凤军,李宝江,高玉刚.果树抗寒性的研究进展[J].黑龙江八一农垦大学学报,2003,15(4):23-29:
    160.杨丽芳,黄建全,田淑芬,等.灌溉方式对冬枣生长的影响[J].河北林业科技,2008(2):7-8;
    161.杨玉珍,王列富,彭方仁.蛋白质的变化与植物抗寒性的关系研究进展[J].生物技术通讯,2007,18(4):711-714;
    162.杨志刚.春季珊瑚树芽抗寒物质及抗氧化酶系的变化[J].常熟高专学报,2002,16(2):47-49;
    163.奕翠华,于宪民,杨燕等.沾化冬枣引种栽培试验[J].果农之友,2003(3):21;
    164.于金俊.冬枣丰产管理技术[J].河北林业科技,2006(3):68-69;
    165.余玲,王彦荣,孙建华.环境胁迫对布顿大麦种子萌发及种苗生长发育的影响[J].草业学报,2002,11(4):79-84:
    166.袁炜群.荔枝果皮钙素积累与利用及其与裂果关系研究[D].广东:华南农业大学,2004;
    167.曾现春,瞿卫东.无公害沾化冬枣生产流通技术[J].中国果菜,2005(5):33-34;
    168.曾骧.果树生理学[M].北京:北京农业大学出版社,1992;
    169.曾烨牟,牟蕴慧,甄灿福,等.李、杏远缘杂交种的创造及其利用研究[J].北方园艺,2000,(6):22-23:
    170.张波.冬枣水肥一体化管理技术初探[J].现代农村科技,2010,(3):38-39;
    171.张成军,李捷,范惠萍等.不同杏品种幼果抗寒性研究[J].西北林学院学报,2008,23(3):29-32;
    172.张承林.果实品质与钙素营养[J].果树科学,1996,13(2):119-123;
    173.张继澍.植物生理学[M].西安:世界图书出版公司,1999;
    174.张金凤,朱之悌,张志毅,等.中介亲本在黑白杨派间杂交中的应用[J].北京林业大学学报,2000,22(6):35-38;
    175.张晋.木本植物源农药的开发应用[J].中国野生植物资源,2003,22(3):23-25;
    176.张立功.无公害冬枣生产周年优化管理历[J].果农之友,2006(2):26-27;
    177.张其德,卢从明,冯丽洁.CO2加富对紫花苜蓿光合作用原初光能转换的影响[J].植物学报,1996,38(1):77-82:
    178.张森,潘立忠,王小兵,等.库尔勒香梨叶内主要矿质元素年生长动态变化的研究[J].安徽农学通报,2007,13(8):4143;
    179.张淑梅.冬枣的土肥水管理[J].河北农业科技,2008,(16):42;
    180.张兴,王兴林,冯俊涛.植物性杀虫剂川楝素的开发研究[J].西北农业大学学报,1993,21(4): 1:
    181.张秀梅,孙光明,杜丽清,等.菠萝果实cDNA文库的构建[J].植物生理学通讯,2008,44(6):1146-1148;
    182.张志善,杨自民,申彦杰.枣无公害高效栽培[M].北京:金盾出版社,2004,1;
    183.张志善.山西省枣树裂果情况调查初报[J].经济林研究,1991,9(1):58-61;
    184.赵继荣,毕阳,刘红霞,等.Trizol法提取厚皮甜瓜果实RNA条件的筛选[J].甘肃农业大学学报,2009,44(2):56-59;
    185.赵庆.冬枣无公害生产管理厉[J].河北果树,2005(6):57;
    186.赵善欢,张兴.植物杀虫剂对水稻三化螟的拒食及内吸毒力试验[J].中国农业科学,1982,(2): 55:
    187.赵同科.植物锌营养研究综述与展望[J].河北农业大学学报,1996,19(1):102-106;
    188.赵智慧,周俊义.冬枣、临猗梨枣果实发育期K, Ca、 Fe、 Zn含量变化[J].河北农业大学学报,2006,26(5):20-23;
    189.赵忠义,于德复,董书颖等.枣树优良品种引种及选择试验[J].辽宁林业科技,2001,1:
    190.郑德合.冬枣管理存在问题及其解决办法[J].西北园艺,2005(2):16;
    191.郑瑞杰,王德永,雷鸣.日本栗叶片矿质营养元素含量年动态变化的研究[J].西北林学院学报,2008,23(4):14-17;
    192.周琳,高春英.沾化冬枣在河南的引种试栽报告[J].山西果树,2002(3):25-26;
    193.周正群.冬枣无公害高效栽培技术[M].北京:中国农业出版社,2002;
    194.朱长青.杨梅果实EST文库构建[D].杭州:浙江大学,2008;
    195.朱根海,刘祖祺,朱培仁.应用Logistic方程确定植物组织低温半致死温度的研究[J].南京农业大学学报,1986,3:11-16;
    196.朱根海,朱培仁.小麦抗冻性的季节变化以及温度对脱锻炼的效应[J].南京农学院学报,1984,(2): 9-17:
    197.朱淑仙,刘善勇,张广霞.冬枣的无公害生产[J].四川林业科技,2005,26(4):100-104;
    198.朱天征.沾化冬枣高产优质的气候条件分析[J].山东气象,2002,22(1):34-36;
    199.朱馨蕾,马艳,张富春.盐胁迫下胡杨cDNA文库的构建及其nhx基因的克隆[J].植物研究,2007,27(1):82-88;
    200.朱艳,郝艳宾,王克建,等.核桃EST-SSR信息分析与标记的初步建立[J].果树学报,2009,26(3):394-398:
    201.朱振东,贾继增.小麦SSR标记的发展及应用[J].遗传,2003,25:355-360;
    202. Agrawal GK, Rakwal R, Iwahwshi H, et al. Isolation of novel riee (oryza sativa L.) multiple stress responsive MAP kinase gene, osMSRMK2, whose mRNA aceumulates rapidly in response to environmental cues[J]. Bioehemical and BioPhysieal Researeh Communieatlons,2002,294(5): 1009-1016;
    203. Chen C X, Zhou P, Choi Y A, Huang S, et al. Mining and characterizing microsatellites from citrus ESTs [J]. Theoretical and Applied Genetics,2006,112:1248-1257;
    204. Diatchenkol, Lauyf, Campbellap, et al. Suppression Subtractivehybridization:A method for generating differentially regulated or tissue specific cDNA probes and libraries[J]. Proc Natl Sci USA, 1996,93(12):6025-6030;
    205. Dorais M, Dermers D A, Papadopoulos A P, et al. Greenhouse tomato fruit cuticle cracking[J]. Horticult Rev.,2004,30,163-184;
    206. Eujayl I, Sorrells ME, Baum M, et al. Isolation of EST-derived microsatellite markers for genotyping the A and B geomes of wheat[J]. Theoretical and Applied Genetics,2002,104:399-407;
    207. Han Z G, Wang C B, Song X L, et al. Characteristics, development and mapping of Gossypium hirsutum derived EST-SSRs in allotetraploid cotton[J]. Theoretical and Applied Genetics,2006,112: 430-439;
    208. Hatey F, Tosser-Klopp G, Clouseard-martinato C, et al. ExPressed sequeneed tags for genes:a review[J]. Genet Sel Evol,1998,30:521-541;
    209. Hofstetter H, Schambock A, Vand BJ, et al. Specific excision of the inserted DNA segment from hybrid plasmids constructed by the poly(dA).poly(dT) method[J].Biochim Biophys Acta,1976,454(3): 587-591;
    210. Jang SH, An KS, Lee SY, et al. Characterization of tobacco MADS-box genes involved in floral initiation [J]. Plant and Cell Physiology,2002,43(2):230-238;
    211. Koerselman W, Meuleman A F M. The vegetation N:P ratio:a new tool to detect the nature of nutrient limitation. Journal of Applied Ecology [J],1996,33:1441-1450;
    212. Niklas K J. Plant biomechanics. an engineering approach to plant form and function[M]. London: The University of Chicago Press,1992;
    213. Opara L U, Studman C J, Banks N H. Physico-mechanical properties of'Gala' apples and stem-end splitting as influenced by orchard management practices and harvest date[J]. Journal of Agricultural Engineering Research,1997,68:139-146;
    214. Ren BZ. Biochemistry and Clinical medicine [M]. Changsha, Hunan Sience and Technical Press, 1993;
    215. Scott K D, Eggler P, Seaton G, Rossetto M, et al. Analysis of SSRs derived from grape ESTs[J]. Theoretical and Applied Genetics,2000,100:723-726;
    216. Sedov EN. Practical relationship of I.V. Michurin sideas in breeding work with apples and pears[J]. Vestnik sel skokhozyaistvennoi Nauki,1980,10:61-67;
    217. Sekse L. Fruit cracking in Norwegian grown sweet cherries[J]. Acta Agriculturae Scandinavica, 1987,37(3):325-328;
    218. Shoferistov E P. Use of distant hybridization in breeding nectarine[J]. Otdalennaya gibridizatsiya I ee rol, vintensifikatsii sadovodstva.1989,76-81:18 ref;
    219. Springer, T.L. McGraw, R.L. Aiken, G.E. Variation of condensed tannins in roundhead lespedeza germplasm [J]. Crop Science,2002,42 (6):2157-2160;
    220. Tessier J T, Raynal D J. Use of nitrogen to phosphorus ratios in plan t tissue as an indicator of nutrient limitation an d nitrogen saturation[J]. Journal of Applied Ecology,2003,40:523-534;
    221. Ureta F, Boidron JN, Bounard J. Influence of dessechement delarafle on grape quality[J]. Amer Enol Vitic,1981,32(2):90-92;
    222. Varshney RK, Graner A, Sorrells ME. Genic mierosatellite marker sin plants:features and applieations[J]. Trends in Bioteehllology,2005,23:48-55;
    223. Wayne Powell, Gordon C. Machray, Jim Provan. Polymorphism revealed by simple sequence repeats[J]. Trends Plant Science,1996,1:215-222;
    224. Weichert H, Jagemann C V, Peschel S, et al. Studies on water transport through the sweet cherry fruit surface:Ⅷ. Effect of selected cations on water uptake and fruit cracking[J]. J. Amer. Soc. Hort. Sci.,2004,129(6):781-788
    225. Xie H, Sui Y, Chang F Q, et al. SSR allelic variation in almond(Prunus dulcis Mill.)[J]. Theoretical and Applied Genetics,2006,112:366-372;
    226. Yamaguchi M, Sato I, Ishiguro M. Influences of epidermal cell size and flesh firmness on cracking susceptibility in sweet cherry (Prunus avium L.) cultivars and selections[J]. Journal of the Japanese Society for Horticultural Science,2002,71:738-746;
    227. Zuojian Tang, Jeong-Hyeon Choi, Chris Hemmerich, et al. ESTPiper-a web-based analysis pipeline for expressed sequence tags[J]. BMC Genomics 2009,10:174.

© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号

地址:北京市海淀区学院路29号 邮编:100083

电话:办公室:(+86 10)66554848;文献借阅、咨询服务、科技查新:66554700