分根交替灌溉对大棚草莓生长、光合作用和氧化酶活性的影响
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摘要
本研究以草莓为试验材料,于2007年-2008年通过在温室大棚盆栽试验,探讨了APRI处理对草莓体内SOD、POD、CAT的活性,MDA、可溶性蛋白的含量、光合作用以及对生长和产量的影响。目的是通过研究APRI处理对生理生化指标,生长状态和产量的影响,探讨APRI在设施草莓生产上的应用价值及APRI技术的实用性和可操作性。研究结果如下:
(1)分根灌溉和完全灌溉处理的草莓,在灌溉后短期内光合作用均受到抑制,这种抑制是非气孔限制作用造成的。APRI处理的草莓在4小时后,这种抑制就被缓解,草莓的光合作用逐渐升高。
(2)就APRI处理整个过程而言,草莓的光合作用有一定的减小,这种减小是因为气孔限制造成的。
(3)APRI处理对草莓灌溉后快速恢复光合作用有一定的促进,这一作用是通过提高草莓对环境变化的适应能力,缓解非气孔限制而体现的。灌溉后,APRI处理的草莓体内SOD、POD、CAT的活性、MDA、可溶性蛋白含量的变化与完全干旱处理相比较都没有剧烈的变化,这说明APRI处理的草莓具有更强的抗逆性。
(4) APRI处理对草莓体内SOD、CAT、POD的活性和MAD、可溶性蛋白含量及其变化范围没有对草莓造成不可恢复性伤害。经过连续15天的测定表明,APRI处理的草莓体内各种酶的活性,MDA可溶性蛋白的含量都没有剧烈的变,并且在灌溉复水后能快速恢复到正常水平,所以APRI对草莓没有不可恢复性伤害,草莓能够正常生长。
(5) APRI处理提高了草莓各器官的干物质含量。经过测定比较,APRI处理的草莓根、茎、叶中干物质的含量均有增加。
(6) APRI处理对草莓的产量没有显著的影响。APRI处理的草莓比正常浇灌的草莓节约用水47.12%。APRI处理在草莓生产上具有节水的实际意义,并且确实可行,具有推广的价值。
In this experimentation, strawberry as the material of experimentation, from 2007 to 2008 has been planted in a greenhouse. We researched the effect of APRI on Strawberry in the activities of SOD, POD, CAT, the content of soluble protein and MDA, photosynthesis, physiological and biochemical targets, the state of growth and output, respectively. The objective is through researching physiological and biochemical targets, the state of growth and output, to discusse the practicality and maneuverability of APRI on the production of strawberry. The results were as follows:
(1) Two kinds of irrigation methods, after irrigation shortly photosynthesis was limited, it was the result of nostomatal limit. The limit on photosynthesis of strawberry with APRI treated in four hours, was unchained, photosynthesis gradually increased.
(2) For the whole process of APRI, the photosynthesis of strawberry decreased, which was the result of stomatal limit.
(3) APRI could renew the photosynthesis after irrigation, which carried out by raising the ability of adapting the strawberry to environmental changes, and reducing the limited of nostomatal. After irrigation, the activity changes of SOD, POD and CAT, the content of MDA, soluble protein of the Strawberry under APRI treatments were abated, which showed that the strawberries under APRI treatments had the stronger resistance.
(4) The change of the activity of SOD, CAT, POD and the content of MAD and soluble protein were in the control of strawberry with APRI and did not cause irreversible injury. After 15 days consecutive mensuration, it showed that the activity of SOD, CAT, POD and the content of MAD, soluble protein had not drastic change, and after irrigation they could quickly return to normal levels, so the injury of APRI on strawberries could resume, strawberry could grow normally.
(5) APRI increased the content of dry matter in various organs of strawberry. The dry matter in roots, stems and leaves of strawberry was increased.
(6) APRI had not obvious affected on the production of strawberry. The yield of strawberry with APRI saved water by 47.12 %. APRI in planting strawberry has the real significance of saving water, doable and being generalized.
(1)分根灌溉和完全灌溉处理的草莓,在灌溉后短期内光合作用均受到抑制,这种抑制是非气孔限制作用造成的。APRI处理的草莓在4小时后,这种抑制就被缓解,草莓的光合作用逐渐升高。
(2)就APRI处理整个过程而言,草莓的光合作用有一定的减小,这种减小是因为气孔限制造成的。
(3)APRI处理对草莓灌溉后快速恢复光合作用有一定的促进,这一作用是通过提高草莓对环境变化的适应能力,缓解非气孔限制而体现的。灌溉后,APRI处理的草莓体内SOD、POD、CAT的活性、MDA、可溶性蛋白含量的变化与完全干旱处理相比较都没有剧烈的变化,这说明APRI处理的草莓具有更强的抗逆性。
(4) APRI处理对草莓体内SOD、CAT、POD的活性和MAD、可溶性蛋白含量及其变化范围没有对草莓造成不可恢复性伤害。经过连续15天的测定表明,APRI处理的草莓体内各种酶的活性,MDA可溶性蛋白的含量都没有剧烈的变,并且在灌溉复水后能快速恢复到正常水平,所以APRI对草莓没有不可恢复性伤害,草莓能够正常生长。
(5) APRI处理提高了草莓各器官的干物质含量。经过测定比较,APRI处理的草莓根、茎、叶中干物质的含量均有增加。
(6) APRI处理对草莓的产量没有显著的影响。APRI处理的草莓比正常浇灌的草莓节约用水47.12%。APRI处理在草莓生产上具有节水的实际意义,并且确实可行,具有推广的价值。
In this experimentation, strawberry as the material of experimentation, from 2007 to 2008 has been planted in a greenhouse. We researched the effect of APRI on Strawberry in the activities of SOD, POD, CAT, the content of soluble protein and MDA, photosynthesis, physiological and biochemical targets, the state of growth and output, respectively. The objective is through researching physiological and biochemical targets, the state of growth and output, to discusse the practicality and maneuverability of APRI on the production of strawberry. The results were as follows:
(1) Two kinds of irrigation methods, after irrigation shortly photosynthesis was limited, it was the result of nostomatal limit. The limit on photosynthesis of strawberry with APRI treated in four hours, was unchained, photosynthesis gradually increased.
(2) For the whole process of APRI, the photosynthesis of strawberry decreased, which was the result of stomatal limit.
(3) APRI could renew the photosynthesis after irrigation, which carried out by raising the ability of adapting the strawberry to environmental changes, and reducing the limited of nostomatal. After irrigation, the activity changes of SOD, POD and CAT, the content of MDA, soluble protein of the Strawberry under APRI treatments were abated, which showed that the strawberries under APRI treatments had the stronger resistance.
(4) The change of the activity of SOD, CAT, POD and the content of MAD and soluble protein were in the control of strawberry with APRI and did not cause irreversible injury. After 15 days consecutive mensuration, it showed that the activity of SOD, CAT, POD and the content of MAD, soluble protein had not drastic change, and after irrigation they could quickly return to normal levels, so the injury of APRI on strawberries could resume, strawberry could grow normally.
(5) APRI increased the content of dry matter in various organs of strawberry. The dry matter in roots, stems and leaves of strawberry was increased.
(6) APRI had not obvious affected on the production of strawberry. The yield of strawberry with APRI saved water by 47.12 %. APRI in planting strawberry has the real significance of saving water, doable and being generalized.
引文
1柴慈江,王明忠。抗寒砧木对露地越冬葡萄水分变化及安全性的影响[J]。天津农业科学,1996,2(3): 20-21
2柴菊华,贺普超,程廉等中国葡萄野生种对葡萄根癌病的抗性[J]。园艺学报,1997,24,(2): 129-132
3陈继峰。葡萄砧木的抗逆性试验[J]。中外葡萄与葡萄酒,2000(2): 16-17
4陈继峰。葡萄砧木品种的研究现状与展望[J]。果树科学,2000,17(2): 138-146
5陈立松,刘星辉。果树对水分胁迫的反应与适应性[J]。干旱地区农业研究,1999,17(1):88-94
6陈立松,刘星辉。水分胁迫对荔枝叶片内源激素含量的影响闭[J]。热带作物学报, 1999,20(3): 31-35
7陈屏昭,王磊,代勋等。缺磷强光下脐橙过剩能量耗散机制[J]。应用生态学报,2005,16 (6):1061-1066
8陈志辉,张良诚,吴光林,张上隆。水分胁迫对柑橘光合作用的影响[J]。浙江农业大学学报,1992,18: 60-66
9程来亮,罗新书。短期土壤干旱对苹果叶片光合速率日变化的影响[J]。果树科学,1990,7:193-200
10池书敏,李广敏,史吉平,董永华,商振清。玉米抗旱机理研究进展[J]。河北农业大学学报,1997,20(4):11-15
11迟伟,王荣富,张成林。遮荫条件下草蓦的光合特性变化[J],应用生态学报,2001,12 (4 ): 566-568
12储钟稀,毛大璋。捕光chla/b蛋白复合物LHC-I和LHCPa在PS中的调节和分配[J]。植物学报,1986,18(1):69～78。
13杜太生,康绍忠等。果树根系分区交替灌溉研究进展[J]。农业工程学报,2005,21(2):172-176。
14杜太生,康绍忠,闫博远等干旱荒漠绿洲区葡萄根系分区交替灌溉试验研究[J]。农业工程学报2007,23(11):52-58。
15樊小林,张福锁。微张力计在植物根系提水作用及养分效应研究中的应用[A]。土壤与植物营养研究新动态(第二卷)ICJ。北京:北京大学出版社,1995: 127-139
16冯彩平,汪沛洪。水分胁迫对小麦叶片氨基酸组份的影响[J]。天津南开大学出版社,1993: 172-175。
17高秀萍,郭修武,王克等。葡萄砧木抗寒与抗根癌病的研究明[J],园艺学报,1993,20 (4):313-318
18高秀萍,闰继耀,刘恩科,张大鹏。水分胁迫下梨、枣和葡萄叶片中甜菜碱含量的变化[J]。园艺学报,2002,29 ( 3 ) : 268-270
19管雪强,赵世杰,李德全,束怀瑞。干旱胁迫下抑制光呼吸对赤霞珠葡萄的光抑制的影响[J]。园艺学报,2004,31 ( 4 ) : 433—43
20郭延平,周慧芬,曾光辉。水分胁迫对柑橘光合速率和光系统II活性的影响团[J]。应用生态学报,2003,14 ( 6 ):867-870
21贾虎森,李德全,韩玉琴。高等植物光合作用的光抑制研究进展[J]。植物学通报,2000,17(3):218～224。
22江华,师生波,许大全。冬季小麦叶片光合作用对温度响应方式的变化[J]。植物生理学报,2000,26(1):69～74。
23接玉玲,曹敏。千旱条件下ABA与气孔导度和叶片生长的关系[J],植物生理学通讯,1999,5 (5):398-403
24康绍忠,张建华,梁宗锁等。控制性交替灌溉—一种新的农田节水调控思路[J ]。干旱地区农业研究,1997,15 (1): 1- 6。
25康绍忠。张建华。石培泽。等。控制性作物根系分区交替灌的理论与实验[J]。水利学报。2001,11: 80- 86。
26李卫东。桃库源关系中源叶光合作用及其碳水化合物代谢的研究[M]。中国农业大学,2005。
27梁继华,李伏生,唐梅,等分根区交替灌溉对盆栽甜玉米水分及氮素利用的影响[J]农业工程学报,2006,22(10):68-71。
28梁宗锁,康绍忠,高俊凤。植物对土壤信号的感知、传递及其水分利用的控制[J]。干旱地区农业研究,1999,17 (2): 72- 78。
29梁宗锁,康绍忠,张建华等。控制性分根交替灌水对作物水分利用率的影响及节水效应[J ]。中国农业科学,1998,31 (5): 88- 90。
30刘成连,战吉成,原永兵,于翠彬,于龙飞。水杨酸对苹果叶片光合作用的影响[J]。园艺学报,1999,26(4):261-262
31刘凤权,王金生。水杨酸对水稻防卫反应酶系的系统诱导[J]。植物生理学通讯通讯,2002,38(2):]21-123
32刘明池,小岛孝之,田中宗浩,陈航。亏缺灌溉对草莓生长和品种的影响[J]。园艺学报,2001,28(4):307-311
33刘新,张蜀秋。水杨酸、茉莉酸和乙烯在调控蚕豆气孔运动中的相互关系[J]。植物生理学报,2000,26 (6):487-491
34刘艳,王丽雪,王有年。水分胁迫对苹果叶片叶绿体1,6一二磷酸果糖磷酸酷酶活性的影响[J]。内蒙古农业大学学报,2002,23: 42-45
35刘祖祺,张石城主编。植物抗性生理学[M]。北京:中国农业出版社,1994: 1-83
36卢从明,唐崇钦,张其德,匡廷云。水分胁迫对小麦叶绿体色素蛋白复合体的影响[J],植物学报,1995,37(12):950-955。
37卢从明,张其德,匡廷云,王忠,高煌珠。水分胁迫抑制水稻光合作用的机理[J]。作物学报,1994,20(5):601-606
38卢从明,张其德,匡廷云。水分胁迫对小麦光系统II的影响[J]。植物学报,1994,36 (2 ): 93-98
39卢从明,张其德,匡廷云。水分胁迫对小麦绿体激发能分配和光系统II原初光能转换效率的影响[J]。生物物理学报,1995,11(1): 82--86
40卢从明,张其德,匡廷云。水分胁迫对小麦叶绿素a荧光诱导动力学的影响[J]。生物物理学报,1993,9(3):453-457
41吕长平,石雪晖等。水分胁迫对草墓叶片SOD活性以及MDA和Vc含量的影响[J]。湖南农业大学学报,1996,22(5):451-455
42潘瑞炽植物生理学(第四版)。北京:高等教育出版社,2001
43蒲光兰,周兰英,胡学华,邓家林,刘永红,肖千文。干旱胁迫对金太阳杏叶绿素荧光动力学参数的影响[J]。干旱地区农业研究,2005, 23: 44-48
44山仑,徐萌。节水农业及其生理生态基础[J]。应用生态学报,1991,2 (1): 70- 76。
45山仑。旱地农业技术发展趋向[J ]。中国农业科学,2002,35 (7): 848- 855。
46史胜青,袁玉欣,张金香,施征。不同水分胁迫方式对核桃苗叶绿素荧光动力学特性的影响[J]。河北农业大学学报,2003,26: 20—24
47史文娟,康绍忠。控制性作物根系分区供水的节水机理及研究进展[J]。水科学进展。2001,2(12): 270- 275。
48束怀瑞。果树栽培生理学[M],北京:农业出版社,1993,118-135
49孙景生,康绍忠。我国水资源利用现状与节水灌溉发展对策。http://www.firi.com. Cn/add/zhjt/zhjt02. htm 2003207226
50孙景生,康绍忠等。控制性交替灌溉研究的进展[J]。农业工程学报,2001,17(4):1-3
51唐晓蕴。部分根系干旱对柑橘光合作用的影响[J ]。园艺学进展,1994, (1): 374- 377。
52徐志防,罗广华,王爱国等。光合作用的光抑制与光合器官的活性氧化代谢[J]。植物生理学,1998,35(4):325～332。
53许大全,沈允钢。光合作用的限制因素[M]。余叔文,汤章城主编,植物生理与分子生物学(第二版)。北京:科学出版社,1998,262-276
54许大全。光合作用气孔限制分析中的一些问题[J]。植物生理学通。1997, 33 (4 ): 241-244
55许明丽,孙晓艳,文江祁。实验室对水分胁迫下小麦幼苗叶片膜损伤的保护作用[J]。植物生理学通讯,2000,36(1):35-36
56许少全,张玉忠,张荣铣。植物光合作用的光抑制[J]。植物生理学通讯,1992,28: 237 -243
57许旭旦,朱涵素。植物根部的水分倒流现象[J]。植物生理学通迅, 1995, 31(4): 241-45。
58杨洪强,接玉玲。果树对地上部的调控及其与水分利用效率的关系[ J ]。园艺学报,2001,28[增]:603- 608。
59郁怡汶草莓光合作用对水分胁迫响应的生理机制研究[M]浙江大学2003
60张守仁。叶绿素荧光动力学参数的意义及讨论[J]。植物学通报,1999,16: 444-448
61邹养军,魏钦平,李嘉瑞等。根系分区灌水对苹果生理功能的影响及控梢促花效应研究[J]。灌溉排水学报。2007,26(4):28-31。
62 Baker, J M , Van Baver C H M. Water transfer through cotton plants connecting soil regions of differing water potential[J]. Argon J , 1998, 80: 993～997.
63 C S Tan, B R Buttery. The effect of soil moisture stress to various fractions of the root system in transpiration, photosynthesis, and internal water relations of peach seedlings [J]. Am Soc Aortic Sic.1982, 107:845-849.
64 Cantore V, Boari F and Caliandro A. Effect of split-root-system water stress on physiological and morphological aspects of pepper (Capsicum annul L.) [J]. Acta Hort. 2000, 573: 321-328
65 Claudio Lovisolo, Wolfram hurting and Andre Schubert. Whole-plant hydraulic conductance and root-to–shoot flow of abscises acid are independently affected by water stress in grapevines [J].Functional Plant Biology, 2002,29:1349-1356.
66 Davies WJ, Bacon MA, Thompson DS and Obeah W and Rodriguez LC. Regulation of leaf and fruit growth in plants growing in drying soil: exploitation of the plants’chemical signaling system and hydraulic architecture to increase the efficiency of water use in agriculture[J]. Exp. Bot, 2000, 51: 1617-1626
67 Dry P R, Lovey B R. Grapevine shoot growth and stomata conductance are reduced when part of the root system is dried [J]. Vitis, 1999, 38 (4): 151- 156.
68 Dry PR, Loveys BR, Botting DG and During H. Effects of partial root-zone drying in grapevine vigor, yield, composition of fruit and use of water [J]. Proc, of 9’Th Oust. Wine Ind. Tech. Conf.1995, 128-131
69 Fort C, Fauveau ML, Muller F, Label P, Granier A, Dreyer E. Stomata conductance growth and root signaling in young oak seedlings subjected topartial soil drying[J]. Tree physiology. 1997, 17:281-289
70 Franks PJ and Farquhar GD. The Effect of Exogenous Abscises Acid on Stomata Development, Stomata Mechanics, and Leaf Gas Exchange in Tradescantia virginiana[J]. Plant Physiol, 2001, 125: 935-942
71 Glenn DM. Physiological effect of incomplete root-zone wetting on plant growth and their implication for irrigation management [J]. HortScience, 2000, 35: 1041-1042
72 Gomez-Cadenas A,Tadeo F R. Talon M,et al. Leaf abscission induced by ethylene in water stressed intact seedling of Cleopatra Mandarin requires previous abscisic acid accumulation in root[J ] .Plant Physiology, 1996, 42(17):1876- 1881.
73 Gowing G, Davies W J, Jones H G. A positive root sourced signal as an indicator of soil drying in apple, Malus×domestic Bork [J]. Journal of Experimental Botany, 1990,41(233):1535-1540.
74 Green S R, Clothier B E. Root water up take by Kiwifruit vines following partial wetting of the root zone [J]. Plant and Soil, 1995, 173:317-328
75 Growing G, Davies W J , Jones H G. A positive root sourced signal as an indicator of soil drying in app le, Malus domestics Borkh [J]. J Exp Bo t, 1990, 41 (233): 1535 -1540.
76 H Biclorai. The effect of Partial wetting of the root zone on yield and water used efficiency in a drip-and sprinkler-irrigated mature grapefruit grove [J].Irrigation- science. 1982, (3): 89-100.
77 Hartung W and Davies WJ. Drought-induced changes in physiology and ABA. In Abscisic acid: physiology and biochemistry [J]. Eds. Davies WJ. Bios Scientific Publishers, Oxford, 1991. 63-79
78 Haupt-Herting S and Fock HP. Oxygen exchange in relation to carbon assimilation in water-stressed leaves during photosynthesis[J]. Ann. Bot, 2002, 89: 851-9
79 Holbrook NM, Shashidhar VR, James RA and Munns R.Stomatal control in tomato with ABA-deficient roots: response of grafted plants to soildrying[J]. Exp. Bot., 2002, 53:1503-1514
80 John V P. The influence of controlled water inputs on grape quality in regions of Australia with hot Mediterranean climates [J ] Acta Hort, 2000, 582: 101- 106.
81 Jones RJ and Mansfield TA. Suppression of stomata opening in leaves treated with abscisic acid[J].1970, Exp. Bot. 21: 714-719
82 Kang Shaozhong. Hu Xiaotao, et al. The effects of partial root zone drying on root, trunk sap flow and water balance in an irrigated pear(Pros communist L.)orchard[J]. Journal of Hydrology. 2003, 280( 1-4):192- 206.
83 Kang Shaozhong. Liang Zongsuo, Hu Wei, etal. Water use efficiency of controlled alternate irrigation on root divided maize p1ants [J]. Agric Water Management, 1998,38: 69-77.
84 Lang J and Jones HG Apple orchards In: Water deficit and plant growth. Vol.6. Ed.Kozolowski, TT.Acdemic Press[J], New York.1981, 419-469.
85 Larry R P. Water management and water relations of horticultural crops [J]. HortScience, 2000, 35 (6): 1035 -1036.
86 Larson K, D,Schaffer B,Andersenp C. Handbook of environ mental physiology of fruit crops. Temperate Crops.1994, 1:271-297
87 Little CHA and Eidt DC. Effect of abscisic acid on bud break and transpiration in woody species[J]. Nature, 1968, 220: 498-499
88 Loveys B R, Dry P R, Stoll M, etal. Using plant physiology to improve the water use efficiency of horticultural crops [J]. Acta Hort, 2000, 537:187-197.
89 Loveys B R, Grant J, Dry P R, et al. Progress in the development of partial root zone drying [J]. The Australian Grape Grower and Winemaker, 1997, 404: 18- 20.
90 Loveys B R, Stroll M, Dry P R, etal. Partial root zone drying stimulates stress responses in grapevine to improve water use efficiency while maintaining crop yield and quality [J].Australian Grape grower and Winemaker Technical issue 1998,414
91 Maroco JP, Rodrigues ML, Lopes C and Chaves M.Limitations to leaf photosynthesis in field-grown grapevine under drought-Metabolic and modeling approaches[J]. Plant Biol., 2002, 29: 451-459
92 Minchin PEH, Farrar JF and Thorpe MR. Partitioning of carbon in split root systems of barley: effect of temperature of the root[J]. Exp. Bot., 1994, 45: 1103-1109
93 Mittelheuser CJ.Stomatal closure and inhibition of transpiration induced by (RS)-abscisic acid[J]. Nature, 1969, 221: 281-282
94 Mongi Z, Lawence R P. Response of lit- root sour o range seedlings to Nail and Polyethylene Glycol stresses [J]. J Exp Bo t, 1990, 41 (222): 35- 40.
95 Mun SR. Involvement of abscisic acid in controlling plant growth in soil of low water potential. [J] Aust J Plant Physiol, 1993.20: 425- 437.
96 Neales T F, Masia A, Zhang J, teal. The effects of partial drying part of the root system of Helianthus annulus on the abscisic acid content of the roots, xylem sap and leaves [J].Journal of Experimental Botany 1989, 40, 1113-1120.
97 Reddy AR, Chaitanyaa KV and Vivekanandan M. Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants[J]. Plant Physiology. 2004, 161: 1189-1202
98 Ruth RF, Srinivas SL and Christopher DR. Abscisic acid signaling in seed and sidling[J]. Plant Cell, 2002, 14: S165-183
99 S R Green, B E Clothier. Root water uptake by kiwifruit vines following partial wetting of the root zone [J].Plant and soil. 1995,137: 317-328
100 Stoll M, Jones H G, Infante J M. Leaf gas exchange and grow thin red raspberries is reduced when part of the root system is dried [J]. Acta Horticulture, 2002, 585: 671- 677.
101 Stoll M, Loveys B, Dry P. Hormonal changes induced by partial root zone drying of irrigated grapevine [J]. J Exp Bo t, 2000, 51 (350): 1627- 1634.
102 Tardieu F and Davies WJ. Root-shoot communication and whole-plant regulation[M] In: Water deficits: plant responses from cell to community,Edited by Smith, JAC, Oxford: Bios Scientific Publishers, 1993
103 Tardieu F, Zhang J, Davies W J. W hat information is conveyed by an ABA signal from maize roots in drying field soil [J]. Plant, Cell and Environment, 1992, (15): 185- 191.
104 Tezara W, Mitchell VJ, Driscoll SD, Lawlor DW. Stress inhibits plant photosynthesis by decreasing coupling factor and ATP[J]. Nature, 1999, 1401: 914-917
105 Vu JCV, Gesch RW, Allen LH, Boote KJ and Bowes G, CO2 enrichment delays a rapid, drought induced decrease in Rubrics small subunit transcript abundance[J]. Plant Physiology,1999, 155-139
106 Winger A, Quick WP, Bungard RA, Bailey KJ, Lea PJ and Leegood RC. The role of photorespiration during drought stress: an analysis utilizing barley mutants with reduced activities of photo respiratory enzymes[J]. Plant cell Environ. 1999, 22: 361-373
107 Yamasaki A,Yoneyama T,Tankak K ea tl .Carbon and nitrogen status of flower-induced strawberry as revealed by C13-and N15-tracer studies[J]. Acta horticulturae.2000(514):301-310.
108 Yao C, Moreshet S and Aloni B. Water relations and hydraulic control of stomata behavior in bell pepper plant in partial soil drying[J]. Plant Cell Environ. 2001, 24: 227-235
109 Zhang J, Davies W J. Control of stomata behaviors by abscisic acid which apparently originates in roots [J]. J Exp Bo t, 1987, (38): 1174- 1181.
110 Zhang J, Davies W J. Does ABA in the xylem control the rate of leaf grows thin soil dried maize and sunflower plants [J] Exp Bo t, 1990, (41): 1125- 1132.
111 Zhang J, Davies W J. Increased synthesis of ABA in partially dehydrated root tips and ABA transport from roots to leaves [J]. Journal of Experimental Botany, 1987, 38 (197):2015-2023.
112 Zhu JK. Salt and drought stress signal transduction in plants. Annul[J]. Rev. Plant Biol., 2002, 53:247-273
2柴菊华,贺普超,程廉等中国葡萄野生种对葡萄根癌病的抗性[J]。园艺学报,1997,24,(2): 129-132
3陈继峰。葡萄砧木的抗逆性试验[J]。中外葡萄与葡萄酒,2000(2): 16-17
4陈继峰。葡萄砧木品种的研究现状与展望[J]。果树科学,2000,17(2): 138-146
5陈立松,刘星辉。果树对水分胁迫的反应与适应性[J]。干旱地区农业研究,1999,17(1):88-94
6陈立松,刘星辉。水分胁迫对荔枝叶片内源激素含量的影响闭[J]。热带作物学报, 1999,20(3): 31-35
7陈屏昭,王磊,代勋等。缺磷强光下脐橙过剩能量耗散机制[J]。应用生态学报,2005,16 (6):1061-1066
8陈志辉,张良诚,吴光林,张上隆。水分胁迫对柑橘光合作用的影响[J]。浙江农业大学学报,1992,18: 60-66
9程来亮,罗新书。短期土壤干旱对苹果叶片光合速率日变化的影响[J]。果树科学,1990,7:193-200
10池书敏,李广敏,史吉平,董永华,商振清。玉米抗旱机理研究进展[J]。河北农业大学学报,1997,20(4):11-15
11迟伟,王荣富,张成林。遮荫条件下草蓦的光合特性变化[J],应用生态学报,2001,12 (4 ): 566-568
12储钟稀,毛大璋。捕光chla/b蛋白复合物LHC-I和LHCPa在PS中的调节和分配[J]。植物学报,1986,18(1):69～78。
13杜太生,康绍忠等。果树根系分区交替灌溉研究进展[J]。农业工程学报,2005,21(2):172-176。
14杜太生,康绍忠,闫博远等干旱荒漠绿洲区葡萄根系分区交替灌溉试验研究[J]。农业工程学报2007,23(11):52-58。
15樊小林,张福锁。微张力计在植物根系提水作用及养分效应研究中的应用[A]。土壤与植物营养研究新动态(第二卷)ICJ。北京:北京大学出版社,1995: 127-139
16冯彩平,汪沛洪。水分胁迫对小麦叶片氨基酸组份的影响[J]。天津南开大学出版社,1993: 172-175。
17高秀萍,郭修武,王克等。葡萄砧木抗寒与抗根癌病的研究明[J],园艺学报,1993,20 (4):313-318
18高秀萍,闰继耀,刘恩科,张大鹏。水分胁迫下梨、枣和葡萄叶片中甜菜碱含量的变化[J]。园艺学报,2002,29 ( 3 ) : 268-270
19管雪强,赵世杰,李德全,束怀瑞。干旱胁迫下抑制光呼吸对赤霞珠葡萄的光抑制的影响[J]。园艺学报,2004,31 ( 4 ) : 433—43
20郭延平,周慧芬,曾光辉。水分胁迫对柑橘光合速率和光系统II活性的影响团[J]。应用生态学报,2003,14 ( 6 ):867-870
21贾虎森,李德全,韩玉琴。高等植物光合作用的光抑制研究进展[J]。植物学通报,2000,17(3):218～224。
22江华,师生波,许大全。冬季小麦叶片光合作用对温度响应方式的变化[J]。植物生理学报,2000,26(1):69～74。
23接玉玲,曹敏。千旱条件下ABA与气孔导度和叶片生长的关系[J],植物生理学通讯,1999,5 (5):398-403
24康绍忠,张建华,梁宗锁等。控制性交替灌溉—一种新的农田节水调控思路[J ]。干旱地区农业研究,1997,15 (1): 1- 6。
25康绍忠。张建华。石培泽。等。控制性作物根系分区交替灌的理论与实验[J]。水利学报。2001,11: 80- 86。
26李卫东。桃库源关系中源叶光合作用及其碳水化合物代谢的研究[M]。中国农业大学,2005。
27梁继华,李伏生,唐梅,等分根区交替灌溉对盆栽甜玉米水分及氮素利用的影响[J]农业工程学报,2006,22(10):68-71。
28梁宗锁,康绍忠,高俊凤。植物对土壤信号的感知、传递及其水分利用的控制[J]。干旱地区农业研究,1999,17 (2): 72- 78。
29梁宗锁,康绍忠,张建华等。控制性分根交替灌水对作物水分利用率的影响及节水效应[J ]。中国农业科学,1998,31 (5): 88- 90。
30刘成连,战吉成,原永兵,于翠彬,于龙飞。水杨酸对苹果叶片光合作用的影响[J]。园艺学报,1999,26(4):261-262
31刘凤权,王金生。水杨酸对水稻防卫反应酶系的系统诱导[J]。植物生理学通讯通讯,2002,38(2):]21-123
32刘明池,小岛孝之,田中宗浩,陈航。亏缺灌溉对草莓生长和品种的影响[J]。园艺学报,2001,28(4):307-311
33刘新,张蜀秋。水杨酸、茉莉酸和乙烯在调控蚕豆气孔运动中的相互关系[J]。植物生理学报,2000,26 (6):487-491
34刘艳,王丽雪,王有年。水分胁迫对苹果叶片叶绿体1,6一二磷酸果糖磷酸酷酶活性的影响[J]。内蒙古农业大学学报,2002,23: 42-45
35刘祖祺,张石城主编。植物抗性生理学[M]。北京:中国农业出版社,1994: 1-83
36卢从明,唐崇钦,张其德,匡廷云。水分胁迫对小麦叶绿体色素蛋白复合体的影响[J],植物学报,1995,37(12):950-955。
37卢从明,张其德,匡廷云,王忠,高煌珠。水分胁迫抑制水稻光合作用的机理[J]。作物学报,1994,20(5):601-606
38卢从明,张其德,匡廷云。水分胁迫对小麦光系统II的影响[J]。植物学报,1994,36 (2 ): 93-98
39卢从明,张其德,匡廷云。水分胁迫对小麦绿体激发能分配和光系统II原初光能转换效率的影响[J]。生物物理学报,1995,11(1): 82--86
40卢从明,张其德,匡廷云。水分胁迫对小麦叶绿素a荧光诱导动力学的影响[J]。生物物理学报,1993,9(3):453-457
41吕长平,石雪晖等。水分胁迫对草墓叶片SOD活性以及MDA和Vc含量的影响[J]。湖南农业大学学报,1996,22(5):451-455
42潘瑞炽植物生理学(第四版)。北京:高等教育出版社,2001
43蒲光兰,周兰英,胡学华,邓家林,刘永红,肖千文。干旱胁迫对金太阳杏叶绿素荧光动力学参数的影响[J]。干旱地区农业研究,2005, 23: 44-48
44山仑,徐萌。节水农业及其生理生态基础[J]。应用生态学报,1991,2 (1): 70- 76。
45山仑。旱地农业技术发展趋向[J ]。中国农业科学,2002,35 (7): 848- 855。
46史胜青,袁玉欣,张金香,施征。不同水分胁迫方式对核桃苗叶绿素荧光动力学特性的影响[J]。河北农业大学学报,2003,26: 20—24
47史文娟,康绍忠。控制性作物根系分区供水的节水机理及研究进展[J]。水科学进展。2001,2(12): 270- 275。
48束怀瑞。果树栽培生理学[M],北京:农业出版社,1993,118-135
49孙景生,康绍忠。我国水资源利用现状与节水灌溉发展对策。http://www.firi.com. Cn/add/zhjt/zhjt02. htm 2003207226
50孙景生,康绍忠等。控制性交替灌溉研究的进展[J]。农业工程学报,2001,17(4):1-3
51唐晓蕴。部分根系干旱对柑橘光合作用的影响[J ]。园艺学进展,1994, (1): 374- 377。
52徐志防,罗广华,王爱国等。光合作用的光抑制与光合器官的活性氧化代谢[J]。植物生理学,1998,35(4):325～332。
53许大全,沈允钢。光合作用的限制因素[M]。余叔文,汤章城主编,植物生理与分子生物学(第二版)。北京:科学出版社,1998,262-276
54许大全。光合作用气孔限制分析中的一些问题[J]。植物生理学通。1997, 33 (4 ): 241-244
55许明丽,孙晓艳,文江祁。实验室对水分胁迫下小麦幼苗叶片膜损伤的保护作用[J]。植物生理学通讯,2000,36(1):35-36
56许少全,张玉忠,张荣铣。植物光合作用的光抑制[J]。植物生理学通讯,1992,28: 237 -243
57许旭旦,朱涵素。植物根部的水分倒流现象[J]。植物生理学通迅, 1995, 31(4): 241-45。
58杨洪强,接玉玲。果树对地上部的调控及其与水分利用效率的关系[ J ]。园艺学报,2001,28[增]:603- 608。
59郁怡汶草莓光合作用对水分胁迫响应的生理机制研究[M]浙江大学2003
60张守仁。叶绿素荧光动力学参数的意义及讨论[J]。植物学通报,1999,16: 444-448
61邹养军,魏钦平,李嘉瑞等。根系分区灌水对苹果生理功能的影响及控梢促花效应研究[J]。灌溉排水学报。2007,26(4):28-31。
62 Baker, J M , Van Baver C H M. Water transfer through cotton plants connecting soil regions of differing water potential[J]. Argon J , 1998, 80: 993～997.
63 C S Tan, B R Buttery. The effect of soil moisture stress to various fractions of the root system in transpiration, photosynthesis, and internal water relations of peach seedlings [J]. Am Soc Aortic Sic.1982, 107:845-849.
64 Cantore V, Boari F and Caliandro A. Effect of split-root-system water stress on physiological and morphological aspects of pepper (Capsicum annul L.) [J]. Acta Hort. 2000, 573: 321-328
65 Claudio Lovisolo, Wolfram hurting and Andre Schubert. Whole-plant hydraulic conductance and root-to–shoot flow of abscises acid are independently affected by water stress in grapevines [J].Functional Plant Biology, 2002,29:1349-1356.
66 Davies WJ, Bacon MA, Thompson DS and Obeah W and Rodriguez LC. Regulation of leaf and fruit growth in plants growing in drying soil: exploitation of the plants’chemical signaling system and hydraulic architecture to increase the efficiency of water use in agriculture[J]. Exp. Bot, 2000, 51: 1617-1626
67 Dry P R, Lovey B R. Grapevine shoot growth and stomata conductance are reduced when part of the root system is dried [J]. Vitis, 1999, 38 (4): 151- 156.
68 Dry PR, Loveys BR, Botting DG and During H. Effects of partial root-zone drying in grapevine vigor, yield, composition of fruit and use of water [J]. Proc, of 9’Th Oust. Wine Ind. Tech. Conf.1995, 128-131
69 Fort C, Fauveau ML, Muller F, Label P, Granier A, Dreyer E. Stomata conductance growth and root signaling in young oak seedlings subjected topartial soil drying[J]. Tree physiology. 1997, 17:281-289
70 Franks PJ and Farquhar GD. The Effect of Exogenous Abscises Acid on Stomata Development, Stomata Mechanics, and Leaf Gas Exchange in Tradescantia virginiana[J]. Plant Physiol, 2001, 125: 935-942
71 Glenn DM. Physiological effect of incomplete root-zone wetting on plant growth and their implication for irrigation management [J]. HortScience, 2000, 35: 1041-1042
72 Gomez-Cadenas A,Tadeo F R. Talon M,et al. Leaf abscission induced by ethylene in water stressed intact seedling of Cleopatra Mandarin requires previous abscisic acid accumulation in root[J ] .Plant Physiology, 1996, 42(17):1876- 1881.
73 Gowing G, Davies W J, Jones H G. A positive root sourced signal as an indicator of soil drying in apple, Malus×domestic Bork [J]. Journal of Experimental Botany, 1990,41(233):1535-1540.
74 Green S R, Clothier B E. Root water up take by Kiwifruit vines following partial wetting of the root zone [J]. Plant and Soil, 1995, 173:317-328
75 Growing G, Davies W J , Jones H G. A positive root sourced signal as an indicator of soil drying in app le, Malus domestics Borkh [J]. J Exp Bo t, 1990, 41 (233): 1535 -1540.
76 H Biclorai. The effect of Partial wetting of the root zone on yield and water used efficiency in a drip-and sprinkler-irrigated mature grapefruit grove [J].Irrigation- science. 1982, (3): 89-100.
77 Hartung W and Davies WJ. Drought-induced changes in physiology and ABA. In Abscisic acid: physiology and biochemistry [J]. Eds. Davies WJ. Bios Scientific Publishers, Oxford, 1991. 63-79
78 Haupt-Herting S and Fock HP. Oxygen exchange in relation to carbon assimilation in water-stressed leaves during photosynthesis[J]. Ann. Bot, 2002, 89: 851-9
79 Holbrook NM, Shashidhar VR, James RA and Munns R.Stomatal control in tomato with ABA-deficient roots: response of grafted plants to soildrying[J]. Exp. Bot., 2002, 53:1503-1514
80 John V P. The influence of controlled water inputs on grape quality in regions of Australia with hot Mediterranean climates [J ] Acta Hort, 2000, 582: 101- 106.
81 Jones RJ and Mansfield TA. Suppression of stomata opening in leaves treated with abscisic acid[J].1970, Exp. Bot. 21: 714-719
82 Kang Shaozhong. Hu Xiaotao, et al. The effects of partial root zone drying on root, trunk sap flow and water balance in an irrigated pear(Pros communist L.)orchard[J]. Journal of Hydrology. 2003, 280( 1-4):192- 206.
83 Kang Shaozhong. Liang Zongsuo, Hu Wei, etal. Water use efficiency of controlled alternate irrigation on root divided maize p1ants [J]. Agric Water Management, 1998,38: 69-77.
84 Lang J and Jones HG Apple orchards In: Water deficit and plant growth. Vol.6. Ed.Kozolowski, TT.Acdemic Press[J], New York.1981, 419-469.
85 Larry R P. Water management and water relations of horticultural crops [J]. HortScience, 2000, 35 (6): 1035 -1036.
86 Larson K, D,Schaffer B,Andersenp C. Handbook of environ mental physiology of fruit crops. Temperate Crops.1994, 1:271-297
87 Little CHA and Eidt DC. Effect of abscisic acid on bud break and transpiration in woody species[J]. Nature, 1968, 220: 498-499
88 Loveys B R, Dry P R, Stoll M, etal. Using plant physiology to improve the water use efficiency of horticultural crops [J]. Acta Hort, 2000, 537:187-197.
89 Loveys B R, Grant J, Dry P R, et al. Progress in the development of partial root zone drying [J]. The Australian Grape Grower and Winemaker, 1997, 404: 18- 20.
90 Loveys B R, Stroll M, Dry P R, etal. Partial root zone drying stimulates stress responses in grapevine to improve water use efficiency while maintaining crop yield and quality [J].Australian Grape grower and Winemaker Technical issue 1998,414
91 Maroco JP, Rodrigues ML, Lopes C and Chaves M.Limitations to leaf photosynthesis in field-grown grapevine under drought-Metabolic and modeling approaches[J]. Plant Biol., 2002, 29: 451-459
92 Minchin PEH, Farrar JF and Thorpe MR. Partitioning of carbon in split root systems of barley: effect of temperature of the root[J]. Exp. Bot., 1994, 45: 1103-1109
93 Mittelheuser CJ.Stomatal closure and inhibition of transpiration induced by (RS)-abscisic acid[J]. Nature, 1969, 221: 281-282
94 Mongi Z, Lawence R P. Response of lit- root sour o range seedlings to Nail and Polyethylene Glycol stresses [J]. J Exp Bo t, 1990, 41 (222): 35- 40.
95 Mun SR. Involvement of abscisic acid in controlling plant growth in soil of low water potential. [J] Aust J Plant Physiol, 1993.20: 425- 437.
96 Neales T F, Masia A, Zhang J, teal. The effects of partial drying part of the root system of Helianthus annulus on the abscisic acid content of the roots, xylem sap and leaves [J].Journal of Experimental Botany 1989, 40, 1113-1120.
97 Reddy AR, Chaitanyaa KV and Vivekanandan M. Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants[J]. Plant Physiology. 2004, 161: 1189-1202
98 Ruth RF, Srinivas SL and Christopher DR. Abscisic acid signaling in seed and sidling[J]. Plant Cell, 2002, 14: S165-183
99 S R Green, B E Clothier. Root water uptake by kiwifruit vines following partial wetting of the root zone [J].Plant and soil. 1995,137: 317-328
100 Stoll M, Jones H G, Infante J M. Leaf gas exchange and grow thin red raspberries is reduced when part of the root system is dried [J]. Acta Horticulture, 2002, 585: 671- 677.
101 Stoll M, Loveys B, Dry P. Hormonal changes induced by partial root zone drying of irrigated grapevine [J]. J Exp Bo t, 2000, 51 (350): 1627- 1634.
102 Tardieu F and Davies WJ. Root-shoot communication and whole-plant regulation[M] In: Water deficits: plant responses from cell to community,Edited by Smith, JAC, Oxford: Bios Scientific Publishers, 1993
103 Tardieu F, Zhang J, Davies W J. W hat information is conveyed by an ABA signal from maize roots in drying field soil [J]. Plant, Cell and Environment, 1992, (15): 185- 191.
104 Tezara W, Mitchell VJ, Driscoll SD, Lawlor DW. Stress inhibits plant photosynthesis by decreasing coupling factor and ATP[J]. Nature, 1999, 1401: 914-917
105 Vu JCV, Gesch RW, Allen LH, Boote KJ and Bowes G, CO2 enrichment delays a rapid, drought induced decrease in Rubrics small subunit transcript abundance[J]. Plant Physiology,1999, 155-139
106 Winger A, Quick WP, Bungard RA, Bailey KJ, Lea PJ and Leegood RC. The role of photorespiration during drought stress: an analysis utilizing barley mutants with reduced activities of photo respiratory enzymes[J]. Plant cell Environ. 1999, 22: 361-373
107 Yamasaki A,Yoneyama T,Tankak K ea tl .Carbon and nitrogen status of flower-induced strawberry as revealed by C13-and N15-tracer studies[J]. Acta horticulturae.2000(514):301-310.
108 Yao C, Moreshet S and Aloni B. Water relations and hydraulic control of stomata behavior in bell pepper plant in partial soil drying[J]. Plant Cell Environ. 2001, 24: 227-235
109 Zhang J, Davies W J. Control of stomata behaviors by abscisic acid which apparently originates in roots [J]. J Exp Bo t, 1987, (38): 1174- 1181.
110 Zhang J, Davies W J. Does ABA in the xylem control the rate of leaf grows thin soil dried maize and sunflower plants [J] Exp Bo t, 1990, (41): 1125- 1132.
111 Zhang J, Davies W J. Increased synthesis of ABA in partially dehydrated root tips and ABA transport from roots to leaves [J]. Journal of Experimental Botany, 1987, 38 (197):2015-2023.
112 Zhu JK. Salt and drought stress signal transduction in plants. Annul[J]. Rev. Plant Biol., 2002, 53:247-273