米非司酮与双炔失碳脂或三苯氧胺合用对人早孕绒毛、蜕膜内分泌功能的影响
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摘要
目的：了解米非司酮与双炔失碳酯或三苯氧胺合用对离体培养的人绒毛、蜕膜组织细胞分泌功能及对蜕膜组织细胞因子的影响，以探讨米非司酮分别与两药合同抗早孕的机理。
方法：组织细胞培养，放射免疫测定等。
结果：1、米非司酮与系列浓度双炔失碳酯(100nM，200nM，400nM，800nM)合用48h，绒毛组织培养液中HCG、P、E_2及6-keto-PGF_(1α)水平与对照组相比显著降低(P＜0.01，P＜0.05)，TXB_2水平显著升高(P＜0.01)，6-keto-PGF_(1α)／TXB_2比值显著下降(P＜0.01)。蜕膜组织培养液中E_2水平与对照组相比显著下降(P＜0.05)，TNF-α及IL-2水平上升(P＜0.01，P＜0.05)，较高浓度的双炔失碳酯(400nM，800nM)与米非司酮合用，显著升高TXB_2水平(P＜0.05)。激素及／或细胞因子水平变化与双炔失碳酯剂量相关。2、米非司酮与系列浓度三苯氧胺(10nM，100nM，1000nM，10000nM)合用48h，绒毛组织培养液中HCG、P、E_2及6-keto-PGF_(1α)水平与对照组相比显著下降(P＜0.01，P＜0.05)，TXB，水平显著升高(P＜0.05)，6-keto-PGF_(1α)/FFXB_2比值显著下降(P＜0.01)。蜕膜组织培养液中E_2、6-keto-PGF_(1α)、PRL水平与对照组相比下降(P＜0.01，P＜0.05)，TNF-α水平显著升高(P＜0.01)。激素和／或细胞因子水平变化与三苯氧胺剂
中文摘要
量相关。3、米非司酮与双炔失碳酯合用对蜕膜T’’u－－J。
1卜2、＊X巳水平的影响（与对照组相 bP＜0＊1，P＜0＊）
强于单用米非司酮（与对照组相比无差异尸＞0刀5）。米
非司酮与三苯氧胺合用对蜕膜E卜ITNFJ水平的影响显
著强于米非司酮单独应用 o4，05，P1、01），对蜕膜
6－keto－P＊F；。水平（与村照组十LP＜005，P＜0＊1） 的
影响超过单用米非司酮（与对照组相比尤差异，P功刀引c
结论：米非司酮与双炔夫碳酯或三苯氧胺合用可显
著改变绒毛、蜕膜组织细胞的分泌功能，并改变蜕膜组
织TTNTJ、L 水平。米非司酮与双炔失碳酯或三苯氧
胺合用对绒毛蜕膜分泌激素和细胞因子功能的影响强度
与双炔夫碳酯或三苯氧胺的剂量有关。米非司酮与双炔
失碳酯或三苯氧胺合用对绒毛蜕膜内分泌功能及对蜕膜
细胞因于水平的影响大于米非司酮单独应用。
Objective: To examine the effects of mifepristone combined with anordrin or famoxifen on the endocrine function of chorion and decidua and on levels of TNF- a and IL-2 of decidua in early human pregnancy in vitro for investigating the mechanisms of antigestational action of mifepristone combined with anordrin or tamoxifen.
Methods: The project was studied by the methods of cells culture and RIA.
Results: 1. In the culture medium of chorionic tissue treated with 1000 nM mifepristone and different concentrations of anordrin (100 nM,200 nM ,400 nM ,800 nM) for 48h, levels of HCG P E2 and 6-keto-PGF1 were decreased significantly (P<0.01, P<0.05) while the level of TXB2 was increased significantly (P<0.01) when compared with the control group. The ratio of 6-keto-PGF/TBX2 was declined greatly (P0.01). In the culture medium of decidual tissue treated with the same method the level of E2 was decreased (P<0.05), levels of TNF- a and IL-2 were increased (P0.01, P<0.05),the level of TXB2 was increased in the culture medium containing 1000nM mifepristone and 400nM or 800nM anordrin, when compared with the control
group.LevelsofHCG P ,TXB2, IL-2,TNF-a in culture medium of chorionic and/or decidual tissue related with the dose of anordrin. 2. In the culture medium of chorionic tissue treated with 1000nM mifepristone and different concentrations of tamoxifen for 48h, levels of HCG P E2 and 6-keto-PGF were decreased significantly (P<0.05, P<0.01) while the level of TXB2 increased significantly (P<0.05) when compared with the control group.The ratio of 6-keto-PGF/TXB2 declined significantly (P0.01). In the culture medium of decidual tissue treated with the same method, levels of E2^ 6-keto-PGF PRL were decreased (P0.01, P0.05) while the level of TNF- a was increased significantly(P<0.01) when compared with the control group. Levels of E2 6-keto-PGF TNF-a in culture medium of chocionic and /or decidual tissue were related with the dose of tamoxifen. 3. Effects of mifepristone combined with anordrin on levels of TNF-a , IL-2 TXB2 from decidual tissue(P<0.01, P<0.05, compared with control group) were greater than mifepristone administed alone (no differerce when compared with the control group,P>0.05). Effects of mifepristone combined with tamoxifen on levels of E2 TNF-a from decidual tissue were greater than mifepristone administed alone (P<0.05, P0.01) and effect on 6-keto-PGF level (P0.05, P0.01, compared with the control group) was greater than mifepristone administed alone (no difference when compared with the control group, P>0.05).
Conclusion: Mifeprstone combined with anordrin or tamoxifen affects the endocrine funtion of chorionic and decidul tissue significantly, and changes production of IL-2 TNF- a from decidual tissue. Effects of mifepristone combined with anordrin or tamoxifen on endocine function of chorionic and decidual tissue and on production of cytokine relate with the dose of anordrin or tamoxifen. Effects of mifepristone combined with anordrin or tamoxifen on endocine function of chorionic and decidual tissue and on production of cytokine were greater than mifepristone administed alone.
方法：组织细胞培养，放射免疫测定等。
结果：1、米非司酮与系列浓度双炔失碳酯(100nM，200nM，400nM，800nM)合用48h，绒毛组织培养液中HCG、P、E_2及6-keto-PGF_(1α)水平与对照组相比显著降低(P＜0.01，P＜0.05)，TXB_2水平显著升高(P＜0.01)，6-keto-PGF_(1α)／TXB_2比值显著下降(P＜0.01)。蜕膜组织培养液中E_2水平与对照组相比显著下降(P＜0.05)，TNF-α及IL-2水平上升(P＜0.01，P＜0.05)，较高浓度的双炔失碳酯(400nM，800nM)与米非司酮合用，显著升高TXB_2水平(P＜0.05)。激素及／或细胞因子水平变化与双炔失碳酯剂量相关。2、米非司酮与系列浓度三苯氧胺(10nM，100nM，1000nM，10000nM)合用48h，绒毛组织培养液中HCG、P、E_2及6-keto-PGF_(1α)水平与对照组相比显著下降(P＜0.01，P＜0.05)，TXB，水平显著升高(P＜0.05)，6-keto-PGF_(1α)/FFXB_2比值显著下降(P＜0.01)。蜕膜组织培养液中E_2、6-keto-PGF_(1α)、PRL水平与对照组相比下降(P＜0.01，P＜0.05)，TNF-α水平显著升高(P＜0.01)。激素和／或细胞因子水平变化与三苯氧胺剂
中文摘要
量相关。3、米非司酮与双炔失碳酯合用对蜕膜T’’u－－J。
1卜2、＊X巳水平的影响（与对照组相 bP＜0＊1，P＜0＊）
强于单用米非司酮（与对照组相比无差异尸＞0刀5）。米
非司酮与三苯氧胺合用对蜕膜E卜ITNFJ水平的影响显
著强于米非司酮单独应用 o4，05，P1、01），对蜕膜
6－keto－P＊F；。水平（与村照组十LP＜005，P＜0＊1） 的
影响超过单用米非司酮（与对照组相比尤差异，P功刀引c
结论：米非司酮与双炔夫碳酯或三苯氧胺合用可显
著改变绒毛、蜕膜组织细胞的分泌功能，并改变蜕膜组
织TTNTJ、L 水平。米非司酮与双炔失碳酯或三苯氧
胺合用对绒毛蜕膜分泌激素和细胞因子功能的影响强度
与双炔夫碳酯或三苯氧胺的剂量有关。米非司酮与双炔
失碳酯或三苯氧胺合用对绒毛蜕膜内分泌功能及对蜕膜
细胞因于水平的影响大于米非司酮单独应用。
Objective: To examine the effects of mifepristone combined with anordrin or famoxifen on the endocrine function of chorion and decidua and on levels of TNF- a and IL-2 of decidua in early human pregnancy in vitro for investigating the mechanisms of antigestational action of mifepristone combined with anordrin or tamoxifen.
Methods: The project was studied by the methods of cells culture and RIA.
Results: 1. In the culture medium of chorionic tissue treated with 1000 nM mifepristone and different concentrations of anordrin (100 nM,200 nM ,400 nM ,800 nM) for 48h, levels of HCG P E2 and 6-keto-PGF1 were decreased significantly (P<0.01, P<0.05) while the level of TXB2 was increased significantly (P<0.01) when compared with the control group. The ratio of 6-keto-PGF/TBX2 was declined greatly (P0.01). In the culture medium of decidual tissue treated with the same method the level of E2 was decreased (P<0.05), levels of TNF- a and IL-2 were increased (P0.01, P<0.05),the level of TXB2 was increased in the culture medium containing 1000nM mifepristone and 400nM or 800nM anordrin, when compared with the control
group.LevelsofHCG P ,TXB2, IL-2,TNF-a in culture medium of chorionic and/or decidual tissue related with the dose of anordrin. 2. In the culture medium of chorionic tissue treated with 1000nM mifepristone and different concentrations of tamoxifen for 48h, levels of HCG P E2 and 6-keto-PGF were decreased significantly (P<0.05, P<0.01) while the level of TXB2 increased significantly (P<0.05) when compared with the control group.The ratio of 6-keto-PGF/TXB2 declined significantly (P0.01). In the culture medium of decidual tissue treated with the same method, levels of E2^ 6-keto-PGF PRL were decreased (P0.01, P0.05) while the level of TNF- a was increased significantly(P<0.01) when compared with the control group. Levels of E2 6-keto-PGF TNF-a in culture medium of chocionic and /or decidual tissue were related with the dose of tamoxifen. 3. Effects of mifepristone combined with anordrin on levels of TNF-a , IL-2 TXB2 from decidual tissue(P<0.01, P<0.05, compared with control group) were greater than mifepristone administed alone (no differerce when compared with the control group,P>0.05). Effects of mifepristone combined with tamoxifen on levels of E2 TNF-a from decidual tissue were greater than mifepristone administed alone (P<0.05, P0.01) and effect on 6-keto-PGF level (P0.05, P0.01, compared with the control group) was greater than mifepristone administed alone (no difference when compared with the control group, P>0.05).
Conclusion: Mifeprstone combined with anordrin or tamoxifen affects the endocrine funtion of chorionic and decidul tissue significantly, and changes production of IL-2 TNF- a from decidual tissue. Effects of mifepristone combined with anordrin or tamoxifen on endocine function of chorionic and decidual tissue and on production of cytokine relate with the dose of anordrin or tamoxifen. Effects of mifepristone combined with anordrin or tamoxifen on endocine function of chorionic and decidual tissue and on production of cytokine were greater than mifepristone administed alone.
引文
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2 翁梨驹：米非司酮在妇产科的临床应用．中华妇产科杂志 1999；34(5)：261～264
3 上海市53探亲避孕片临床科研协作组．53探亲避孕片的临床观察．1977；10：608～612
4 Mehta RR, Chatterton Rr. Antiovulatory action of anor-drin in the cynomolgus monkey (macaca fascicularis). Adv Contracept 1991 Mar;7(1):21～8
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