复方丹参配伍的化学物质基础和药理研究
|
摘要
近年来中药研究正在药学界蓬勃兴起。中药复方是中医用药的主要形式,而配伍理论是中药复方的根本。本论文针对中药配伍中的问题,对复方丹参方剂配伍的化学成分变化、药理药效变化及其药效物质基础进行了研究。论文第二章建立了一套稳定、可靠、重现性好、统一的HPLC方法,分析了丹参、三七及复方丹参配伍前后化学成分变化研究。结果表明,复方丹参48个峰中有24个峰来源于丹参,10个峰来源于三七,还有14个峰是共流出峰,但没有新峰产生;与单味药丹参、三七比较,配伍后有19个峰含量增加,6个峰含量降低。
论文第三章考察了丹参、三七按不同比例配伍;丹参、三七用系统溶剂萃取法得到的不同极性组分以及丹参、三七的有效组分按不同比例配伍后的药效变化。结果表明,不同配伍比例药效不同,不同组分药效不同,而以有效组分进行配伍后,有效比例比以生药丹参、三七进行配伍的有效比例要广。为了进一步揭示复方丹参作用的物质基础,论文第四、第五章分别对丹参、三七进行了HPLC-MS/MS分析,在质谱电离和质谱裂解规律的基础上,鉴定了丹参乙酸乙酯组分中28种水溶性成分,氯仿组分中24种脂溶性成分和三七中以皂甙为主的59种成分。
最后,在质谱分析的基础上,对丹参水溶性成分的液相色谱保留参数和峰形参数规律进行了初步研究,发现保留参数规律与化合物结构有关,峰形参数规律与化合物的解离有关。
In the recent years, the research focus on Chinese Traditional Medicine (TCM) is becoming more and more popular in medicine field. Prescriptions are the main form of TCM. Theory of compatibility is the essential part of TCM. In this paper, the changes of chemical components, the changes of pharmacological activities, and the effective compounds in Fufang Danshen were studied.
A stable, reliable, uniform HPLC analytical method with good reproducibility was developed in the second chapter to study the changes of chemical ingredients between single herbs of Salvia miltiorrhizae (SM), Panax notoginseng (PN) and their prescription-Fufang Danshen (FFDS). The results indicated that in the 48 peaks of FFDS, there are 24 peaks contributed from SM,10 peaks contributed from PN, and 14 peaks co-contributed from SM and PN, but no new peaks produced. Comparing FFDS with single herbs of SM and PN, the areas of 19 peaks are increased; while the areas of 6 peaks are decreased.
The influence of different compatible ratios of SM to PN in FFDS, different fractions of SM and PN extracted with different solvent, different compatible ratios of effective fractions of SM to effective fractions of PN in FFDS on pharmacological activity were investigated in the third chapter. The results indicated that different ratios of SM to PN in FFDS have different pharmacological activity; different fractions also have different pharmacological activity; and the effective ratios of effectual fractions of SM to effectual fractions of PN are wider than those of SM to PN.
论文第三章考察了丹参、三七按不同比例配伍;丹参、三七用系统溶剂萃取法得到的不同极性组分以及丹参、三七的有效组分按不同比例配伍后的药效变化。结果表明,不同配伍比例药效不同,不同组分药效不同,而以有效组分进行配伍后,有效比例比以生药丹参、三七进行配伍的有效比例要广。为了进一步揭示复方丹参作用的物质基础,论文第四、第五章分别对丹参、三七进行了HPLC-MS/MS分析,在质谱电离和质谱裂解规律的基础上,鉴定了丹参乙酸乙酯组分中28种水溶性成分,氯仿组分中24种脂溶性成分和三七中以皂甙为主的59种成分。
最后,在质谱分析的基础上,对丹参水溶性成分的液相色谱保留参数和峰形参数规律进行了初步研究,发现保留参数规律与化合物结构有关,峰形参数规律与化合物的解离有关。
In the recent years, the research focus on Chinese Traditional Medicine (TCM) is becoming more and more popular in medicine field. Prescriptions are the main form of TCM. Theory of compatibility is the essential part of TCM. In this paper, the changes of chemical components, the changes of pharmacological activities, and the effective compounds in Fufang Danshen were studied.
A stable, reliable, uniform HPLC analytical method with good reproducibility was developed in the second chapter to study the changes of chemical ingredients between single herbs of Salvia miltiorrhizae (SM), Panax notoginseng (PN) and their prescription-Fufang Danshen (FFDS). The results indicated that in the 48 peaks of FFDS, there are 24 peaks contributed from SM,10 peaks contributed from PN, and 14 peaks co-contributed from SM and PN, but no new peaks produced. Comparing FFDS with single herbs of SM and PN, the areas of 19 peaks are increased; while the areas of 6 peaks are decreased.
The influence of different compatible ratios of SM to PN in FFDS, different fractions of SM and PN extracted with different solvent, different compatible ratios of effective fractions of SM to effective fractions of PN in FFDS on pharmacological activity were investigated in the third chapter. The results indicated that different ratios of SM to PN in FFDS have different pharmacological activity; different fractions also have different pharmacological activity; and the effective ratios of effectual fractions of SM to effectual fractions of PN are wider than those of SM to PN.
引文
[1] 于友华,王永炎,赵宜军,程昭寰. 方剂配伍规律的研究. 中国中药杂志. 2001,26(4):219-222.
[2] 夏征农. 辞海. 上海:辞书出版社. 1999.
[3] 王付. 经方药物药理临证指南. 北京:中医古籍出版社,1999.
[4] 高晓山. 中药配伍研究问题瞻望. 中国实验方剂学杂志. 1999,5 (6):1-7.
[5] 韩笑, 胡一冰, 邱德文. 中药复方配伍规律研究. 中国中医基础医学杂志.2000,6(8):59-64.
[6] 凌一揆,颜正华. 中药学. 上海:上海科学技术出版社. 1986.
[7] 蒋永光,李认书,金桂花,黎勇. 方剂配伍的研究现状及问题分析. 国医论坛. 2002,17 (1):51-52.
[8] 魏国丽,郑学宝. 方剂配伍规律现代科学内涵研究进展. 中医药临床杂志. 2005,17(5):515-517.
[9] 朱天忠. 芍药甘草汤的临床应用及药理研究. 北京中医杂志. 1985,4(4):36-40.
[10] 鲍军. 泻心汤体外抗凝及抗血小板聚集作用的研究. 中成药研究. 1988,(10):28-30.
[11] Yuan D, Komatsu K, Cui Z, Kano Y. Pharmacological properties oftraditional medicines (XXVII). Interaction between ephedra herb and gypsum under hyperthermal conditions in rats. Biol. Pharm. Bull. 2002, 25(7):872-874.
[12] 范颖. 试论中医方剂配伍规律研究的现状与基本思路. 江苏中医药. 2003,24 (4):9-11.
[13] 韩笑,胡一冰,邱德文. 中药复方配伍规律研究. 中国中医基础医学杂志. 2000,6(8):59-64.
[14] 关怀,穆阳,高燕,许艳丽,苏宏宇. 不同配伍剂量对大黄-黄连药对的有效成分及药理作用的影响研究. 北京中医. 2000,2:53-55.
[15] 顿宝生,许爱英. 中医方剂配伍规律的探析. 陕西中医学院学报. 2000,23(6):4-5.
[16] 聂淑琴,杨庆,李兰芳,黄璐琦. 柴胡与赤芍、醋柴胡与白芍配伍前后药效学比较研究. 中国实验方剂学杂志. 2002,8(3):11-14.
[17] 赵建荣, 李晓玫. 中药复方物质基础研究的现状与进展. 中草药. 2003,34(11):963-967.
[18] 吴立军主编. 中药化学. 北京:中国医药科技出版社. 2000.
[19] 陈耀祖. 中药复方化学研究策略诹议. 化学进展. 1999,11(2):188-192.
[20] 刘建利. 中药复方化学研究的思路. 中成药,1992,14(10):49-53.
[21] 何祥久,邱峰,姚新生. 中药复方研究现状和思路. 化学进展. 2001,13(6):481-485.
[22] 汪显阳. 大黄牡丹汤不同配伍对有效成分煎出的影响. 中国中医药科技. 2002,9(3):161-162.
[23] 何伟,王宁,秦林,张翔. 川乌与白芍配伍前后乌头碱和芍药苷煎出量的测定. 中国药学杂志. 2002,37(9):680-683.
[24] 何伟,秦林,司淑媛. 附子与白芍配伍前后乌头碱煎出量的测定. 中草药.2002,33(7):600-602.
[25] 段天璇,马长华,侯艳霞,刘舒萍,陈韬,伦丽辉,小熊亮子,李宇航. 半夏泻心汤不同配伍情况下部分化学成分变化. 中国中药杂志. 2002,27(2):363-365.
[26] 李吉来,陈飞龙,刘传明,罗佳波. 麻黄汤中麻黄碱的 GC-MS 法测定及配伍因素对汤剂中该成分含量的影响. 中草药. 2002,33(4):703-705.
[27] 陈建萍,谭炳炎,吴伟康. 四逆汤中附子甘草配伍规律研究. 中国实验方剂杂志. 2001, 7 (3) ∶16-20.
[28] 肖红斌. 中药复方液相色谱统一方法的研究及其在补阳还伍汤中的应用. 中国科学院大连化学物理研究所博士论文. 1997.
[29] 张子忠. 中药质量控制方法及其配伍复方中化学成分变化规律的应用基础研究. 中国科学院大连化学物理研究所博士论文. 2000.
[30] 李秀玲. 精制血府逐瘀复方配伍的药效物质基础研究. 中国科学院大连化学物理研究所博士论文. 2003.
[31] 朱丹妮,李志明,严永清. 生脉散复方化学的动态变化与药效关系的研究-生脉散复方化学的研究(Ⅱ). 中国中药杂志. 1998,23(5):230-232.
[32] 朱丹妮,李志明,严永清. 生脉散复方化学动态变化与药效关系的研究-生脉散复方化学的研究(Ⅲ). 中国中药杂志. 1998,23(8):291-293
[33] 刘建勋. 中药药效学研究与中医临床相结合. 中药新药与临床药理. 1997,8(3):137-138.
[34] 严永清. 关于加强中药复方化学成分的研究. 中国中药杂志. 1985,10(2):3-5.
[35] 严永清,马晓红,李志明. 生脉散复方化学成分的动态变化与药效的研究(三). 中国学术期刊文摘(科技快报). 1998,4(7):860-862.
[36] 严永清,朱丹妮,李志明. 生脉散复方化学成分的动态变化与药效的研究(五). 中国学术期刊文摘(科技快报). 1998,4(7):865-868.
[37] 宋小莉,高艳青,牛欣,司银楚. 复方配伍实验设计方法评述. 中西医结合学报. 2003,1(3):177-179.
[38] 王昕. 大黄 虫丸的研究及应用概况. 中成药. 1997,19(2):45-46.
[39] 孙洋,陈婷,徐强. 从药对的角度考察复方配伍规律. 世界科学技术-中医药现代化研究. 2004,6(1):17-21.
[40] 吴建新,严永清,蒋莹. 六味地黄汤的化学研究. 现代应用药学. 1990,7(1):16-18.
[41] 蒋莹,赵陆华,严永清. 六味地黄汤及其配伍对过氧化脂质及脂褐质含量的影响. 中国中药杂志. 1991,16(3):175-176.
[42] 宋宗华,戴舒佳,黎辉琴. 苓桂术甘汤配伍机制研究. 中国中药杂志. 2002,27(10):760-762.
[43] 王庆国,李宇航,赵琰. 半夏泻心汤及其拆方对正常大鼠胃肠运动功能的影响. 北京中医药大学学报. 2001,24(6):19-21.
[44] 曹华,何国,李政木. 经方“药对”配伍规律研究的现状及分析. 国医论坛. 2003,18(4):1-3.
[45] 姚新生,胡柯. 中药复方现代化研究. 化学进展. 1999,19(2):192-196.
[46] 袁久荣. 关于方剂学科发展中若干前沿关键问题的研究. 中国中医基础医学杂志. 2005,11(9):681-685.
[47] 余林中,罗佳波,谭晓梅. 方剂配伍规律研究应以物质基础为核心. 中国中医基础医学杂志. 1999, 5(10):18-20.
[48] 国家药典委员会. 中华人民共和国药典,一部. 北京:化学工业出版社,2005
[49] 徐任生主编. 丹参-生物学及其应用. 北京:科学出版社,1990.
[50] 郑虎占,董泽宏,余靖. 中药现代研究与应用(第2卷). 北京:学苑出版社. 庶虫1997,P1093.
[51] 郑光植,杨崇仁编. 三七-生物学及其应用. 北京:科学出版社,1994.
[52] 郑虎占,董泽宏,余靖. 中药现代研究与应用(第 1 卷). 北京:学苑出版社. 1997,P195.
[53] 陈文莉. 三七配伍应用探析. 江西中医药. 2002,33(2):48.
[54] 中国医学科学院药物研究所编著. 中草药现代研究(第2卷). 北京:北京医科大学中国协和医科大学联合出版社. 1996,472-479.
[55] 孔德云. 丹参的化学成分. 中国医药工业杂志. 1989,20(6):279-285.
[56] 郑国墀,柿泽宽. 丹参化学成分的研究概况. 中国药学杂志. 1989,24(1):5-10.
[57] 周长新,罗厚蔚,丹羽正武. 丹参水溶性化学成分的研究. 中国药科大学学报,1999, 30(6): 411-416.
[58] Lu YR, Foo LY. Polyphenolics of salvia-a review. Phytochemistry. 2002, 59,117-140.
[59] Kasimu R, Tezuka Y, Tanaka K, Gong ZN, Li JX, Basnet P, Namba T, Kadota S. Liquid chromatography-mass spectrometry analysis of diterpenoid constituents of seventeen Salvia plants. J. Trad. Med. 1998, 15, 109-115.
[60] Kasimu R, Tanaka K, Tezuka Y, Gong ZN, Li JX, Basnet P, Namba T, Kadota S. Comparative study of seventeen Salvia plants: aldose reducatase inhibitory activity of water and MeOH extracts and liquid chromatrgraphy-mass spectrometry (LC-MS) analysis of water extracts. Chem. Pharm. Bull. 1998, 46, 500-504.
[61] Lin HC, Chang WL. Diterpenoids from salvia miltiorrhiza. Phytochemistry. 2000, 53, 951-953.
[62] Zhang JL, Cui M, He Y, YU HL, Guo DA. Chemical fingerprint and metabolicfingerprint analysis of DanShen injection by HPLC-UV and HPLC-MS methods. J. Pharmaceut. Biomed. 2005, 36, 1029-1035.
[63] Tsuda T, Watanabe M, Ohshima K, Yamamoto A, Kawakishi S. and Osawa. T. Antioxidative components isolated from the seed of tamarind (tamarindus indica L.). J. Agric. Food. Chem. 1994, 42, 2671-2674.
[64] 张建虎,叶芊. 丹参酮药理作用. 中华医学研究杂志. 2005,5(6):51-53.
[65] 陈维洲. 丹参的药理. 药学学报. 1984,19(11):876-880.
[66] Fung KP,Zeng LH,Wu J,Wong HN,Lee CM, Hon PM,Chang HM,Wu TW. Demonstration of the myocardial salvage effect of lithospermic acid B isolated from the aqueous extract of Salvia miltiorrhiza. Life Sci. 1993,52(22):1239-44.
[67] Wu TW,Zeng LH,Fung KP,Wu J,Pang H,Grey AA,Weisel RD,Wang JY. Effect of sodium tanshinone IIA sulfonate in the rabbit myocardium and on human cardiomyocytes and vascular endothelial cells. Biochem Pharmacol. 1993,46(12):2327-2332.
[68] Xin CW. and Michael HG. Antioxidant Activity of Quinones Extracted from Tanshen (Salvia miltiorrhiza Bunge). J. Agric. Food. Chem. 1992,40:1331-1336.
[69] Liu J,Shen HM,Ong CN. Role of intracellar thiol depletion, mitochondrial dysfunction and reactiove oxygen species in Salvia Miltiorrhiza-induced apoptosiss in human hepatoma HepG2 cells. Life Sciences. 2001,69:1833-1850
[70] 中国医学科学院药物研究所编著. 中草药现代研究(第 3 卷). 北京:北京医科大学中国协和医科大学联合出版社. 1996,191-192.
[71] 甘烦远,郑光植. 三七化学成分研究概况. 中国药学杂志. 1992,27(3):138-143.
[72] Yoshikawa M, Murakami T, Ueno T, Yashiro K, Hirokawa N, Murakami N, Yamahara J, Matsuda H, Saijoh R and Uanaka O. Bioactive saponins and glycosides. VIII. Notoginseng (1) New dammarane-type triterpene oligoglycosides, notoginsenosides-A, -B, -C, and -D, from the dried root of Panax notoginseng. Chem Pharm Bull, 1997, 45: 1039-1045.
[73] Yoshikawa M, Murakami T, Ueno T, Hirokawa N, Yashiro K, Murakami N, Yamahara J, Matsuda H, Saijoh R and Uanaka O. Bioactive saponins and glycosides. IX. Notoginseng (2) Structures of five new dammarane-type triterpene oligoglycosides, notoginsenosides-E, -G, -H, -I and -J, and a novel acetylenic fatty acid glycoside, notoginsenic acidβ-sophoroside, from the dried root of Panax notoginseng. Chem Pharm Bull, 1997, 45: 1056-1062.
[74] Yoshikawa M, Morikawa T, Yashiro K, Murakami T, Matsuda H. Bioactive saponins and glycosides. XIX. Notoginseng (3) Immunological Adjuvant activity of Notoginsenosides and related saponins: Structures of notoginsenosides-L, -M and –N form the root of Panax notoginseng (Burk.) F. H. Chen. Chem Pharm Bull, 2001, 49: 1452-1456.
[75] 魏均娴,王菊芬. 三七叶黄酮类成分的研究. 中药通报. 1987,12(1):31-33
[76] Ma WG, Mizutam M, Malterud KE, et al. Saponins from the roots of Panax notoginseng. Phytochemistry. 1999,52:1133-1139.
[77] 杨柳,叶蕴华,邢其毅. 人参中γ-氨基丁酸的分离、鉴定及氨基酸的定量分析. 科学通报. 1991,(7):513-518.
[78] Liu JH,Wang X,Cai SQ,Komatsu K and Namba T. Analysis of the Constituents in the Chinese Drug Notoginseng by Liquid Chromatography-Electrospray Mass Spectrometry. J. Chin. Pharm. Sci. 2004,13(4):225-237.
[79] 李琦. 三七水溶性化学成分及其药理研究新近展. 高等学校化学学报. 1995,17(12):1886-1892.
[80] 蔡燕玲,黎罕文. 三七的药理研究进展. 陕西中医学院学报. 2001,24(5):57-58.
[81] Sun HX, Ye YP, Pan HJ, Pan YJ. Adjuvant effect of Panax notoginseng saponins on the immune responses to ovalbumin in mice. Vaccine. 2004,22:3882–3889.
[82] Li LX, Wang ZC,Huang ZH,Zhang YM and Wei JX. Effects of Panax notoginseng saponins on acute cerebral ischemia. Chin. Pharmacol. Bull. 1991,7:56-59.
[83] Huang C,Gu TX,Guan YY,and Wang JQ. Effects of Panax notoginseng saponins on myocardial ischemia and reperfusion injury in conscious rabbit. Chin. Pharmacol. Bull. 1991,7:190-193.
[84] 刘小清,罗宪,欧阳小安. 复方丹参剂型研究简况. 时珍国医国药. 2001,12(5):474-475.
[85] 罗晓健,毕开顺,周书余. 复方丹参片的研究概况. 中成药. 2001,23(5):371-374.
[86] 王筠默,李仪奎,张海根. 复方丹参片的药理作用研究.中成药研究. 1984,(11):22-24.
[87] 张昱,王绵之,黄启福. 复方丹参片对大鼠心肌缺血再灌注损伤的保护作用. 河南中医刊. 1997,12 (6):15-17.
[88] 吴乃峰,阎希军,朱永宏. 复方丹参滴丸与复方丹参片的药理作用比较. 中成药. 1993,15 (8):24-25.
[89] 刘婷,秦彩玲,张毅,康立源,孙有富,张伯礼. 丹参、三七不同比例配伍对正常家兔血小板聚集性、粘附性的影响. 中国中药杂志. 2002,27(8):609-611.
[90] 商洪才,张伯礼,高秀梅,王怡,李玉红,康立源,杜嵘,江海涛. 丹参三七药对不同配比药效学比较研究. 辽宁中医杂志. 2002,29(5):297-299.
[91] Olesze WA. Chromatographic determination of plant saponins. J. Chromatogr. A. 2002,967:147-162.
[92] Caniova A. and Brandsteterova E. HPLC analysis of phenolic acids in plant material. Chem. Anal.(warsaw). 2001,46:757-780.
[93] Drasar P., Moravcova. J. Recent advances in analysis of Chinese medical plants and traditional medicines. J. Chromatogr. B. 2004, 812:3-21
[94] Yao LH, Jiang YM, Datta N, Singanusong R, Liu X, Duan J, Raymont K, Lisle A, Xu Y. HPLC analyses of flavanols and phenolic acids in the fresh young shoots of tea (Camellia sinensis) grown in Australia. Food Chemistry. 2004,84:253-263.
[95] Li L, Zhang JL, Sheng YX, Guo DA, Wang Q and Guo HZ. Simultaneous quantification of six major active saponins of Panax notoginseng by high-performance liquid chromatography-UV method. J. Pharmaceut. Biomed. 2005,38:45-51.
[96] Lazarowych N.J. and Pekos P. Use of Fingerprinting and marker compounds for inentification and standardiazation of botanical drugs: strategies for applying pharmaceutical HPLC analysis to herbal products. Drug Inf. J. 1998, 32:497-512.
[97] Lu GH, Chan K, Liang YZ, Leung K, Chan CL, Jiang ZH, Zhao ZZ. Development of high-performance liquid chromatographic fingerprints for distinguishing Chinese Angelica from related umbelliferae herbs. JChromatogra. A. 2005,1073:383-392.
[98] Lee MS, Kerns EH. LC-MS applications in drug development. Mass Spectrom. Rev. 1999,18:187-279.
[99] Ye M., Yan Y. N., Guo D. A. Characterization of phenolic compounds in the Chinese herbal drug Tu-Si-Zi by liquid chromatography coupled to electrospray ionization mass spectrometry. Rapid Commun. Mass Spectrom. 2005, 19:1469-1484.
[100] 李秀玲,肖红斌,胡皆汉,梁鑫淼. 液相色谱/质谱/质谱联用鉴定芍药中的一种新化合物. 分析化学. 2003,31(3):329-331.
[101] 王莉,肖红斌,胡皆汉,王加宁,梁鑫淼. 天麻中对羟基苯类化合物的高效液相色谱-质谱研究. 分析化学. 2003,31(8):954-957.
[102] Xiaozhe Zhang, Hongbin Xiao, Qing Xu, Xiuling Li, Jianning Wang, Xinmiao Liang. Characterization of Phthalides in Ligusticum chuanxiong by Liquid Chromatographic-Atmospheric Pressure Chemical Ionization-Mass Spectrometry. J. Chromatogr. Sci. 2003,41:428-433.
[103] Ma XQ, Liang XM, Xu Q, Zhang XZ and Xiao HB. Identification of Ginsenosides in Roots of Panax ginseng by HPLC-APCI/MS. Phytochem. Anal. 2005,16:181-187.
[1] 黄泰康. 中药系统工程体系的建立与发展初探. 科学通报, 1995,40(19):1729-1734.
[2] 阴键,郭力弓主编. 中药现代研究与临床应用,北京:学苑出版社,1993.
[3] 黄泰康主编. 常用中药成分与药理研究,北京:中国医药科技出版社,1994
[4] 刘建利. 中药复方化学研究的思路. 中成药,1992,14(10):49-53.
[5] 王静蓉,马终瑜,严永清等. 芍药甘草配伍化学变化研究. 时珍国医国药,2000,11(2):102-104.
[6] 汪显阳. 大黄牡丹汤不同配伍对有效成分煎出的影响. 中国中医药科技,2002,9(3):161-162.
[7] 何伟,秦林,司淑媛. 附子与白芍配伍前后乌头碱煎出量的测定. 中草药,2002,33(7):600-602.
[8] 陈建萍,谭炳炎,吴伟康,张敏生,李礼娟,韩超. 四逆汤中附子甘草配伍规律研究. 中国实验方剂学杂志,2001,7(3):18-19.
[9] 夏云,李志明,朱丹妮. 生脉散复方化学动态变化与药效关系的研究-生脉散复方化学的研究(Ⅰ).中国中药杂志,1998,23(4):230-232.
[10] 朱丹妮,李志明,严永清. 生脉散复方化学动态变化与药效关系的研究-生脉散复方化学的研究(Ⅱ).中国中药杂志,1998,23(5):291-293.
[11] 朱丹妮,严永清,李志明. 生脉散复方化学动态变化与药效关系的研究-生脉散复方化学的研究(Ⅲ).中国中药杂志,1998,23(8):483-485.
[12] 肖红斌. 中药复方液相色谱统一方法的研究及其在补阳还伍汤中的应用. 中国科学院大连化学物理研究所博士论文,1997.
[13] 张子忠. 中药质量控制方法及其配伍复方中化学成分变化规律的应用基础研究. 中国科学院大连化学物理研究所博士论文,2000.
[14] 李秀玲. 精制血府逐瘀复方配伍的药效物质基础研究. 中国科学院大连化学物理研究所博士论文,2003.
[1] 李庭凯,王晓春,赵勤萍,等. 复方丹参滴丸治疗冠心病心绞痛心血瘀阻证的临床研究. 中西医结合心脑血管病杂志, 2003,1:215-216.
[2] 秦培森. 复方丹参滴丸治疗冠状动脉粥样硬化性心脏病心绞痛临床观察. 中成药. 2000,22:630-632.
[3] 林萍,陈贤岸. 复方丹参片与复方丹参滴丸治疗心绞痛疗效比较及原因分析. 广州医药,2001,32:73.
[4] 杨庆福,李彩玲,陈俭. 复方丹参滴丸对冠心病心绞痛患者血管内皮功能的影响. 新乡医学院学报,2003,20:127-128.
[5] 国家药典委员会. 中华人民共和国药典. 一部. 北京:化学工业出版社. 2005,527-528.
[6] 戈延茹,方亮,李杰. 复方丹参片药典方制剂的研究. 中成药. 2002, 24:872-875.
[7] 王作顺. 复方丹参滴丸的研究进展. 吉林中医药. 2003,23:46-48.
[8] 余世春,琚小龙,段广勋. 丹参的化学成分和药理活性研究概况. 安徽卫生职业技术学院学报,2002,1:43-47.
[9] 丁章森. 丹参的临床药理研究概况. 湖北中医学院学报. 2000,12:56-59.
[10] 徐勇. 丹参对心血管系统的作用及其临床应用. 天津中医学院学报. 1998,17:31.
[11] 杨志刚,陈阿琴,俞颂东. 三七药理研究新进展. 上海中医药杂志. 2005,39:59-62.
[12] 李琦. 三七水溶性化学成分及及药理研究新进展. 高等学校化学学报. 1995,17:1886-1892.
[13] 韩金安, 胡威夷. 三七总皂甙对缺血性脑损伤保护作用的研究进展. 中国中西医结合杂志. 1996,16:506-507.
[14] 陈云红, 施鹤高, 王玉梅. 近年来三七的药理研究进展. 中草药. 1995,26:160-162.
[15] 陈泉生,郑振源,杨士琰,陈古荣. 三七(须根)总皂甙的药理研究. 中药通报. 1987,12:45-47.
[16] 费震宇, 王文健,陈响中. 三七用于冠心病治疗的药理研究.时珍国药研究. 1997,8:17-18.
[17] 孔德云. 丹参的化学成分. 中国医药工业杂志. 1989,20:279-285.
[18] 郑国樨,柿泽宽. 丹参化学成分的研究概况. 中国药学杂志. 1989,24:5-10.
[19] Lu Y-R, Foo LY. Polyphenolics of salvia-a review. Phytochemistry. 2002,59:117-140.
[20] 刘刚,鲍建材,郑友兰,张崇禧. 三七的化学成分研究进展. 人参研究. 2004,2:10-18.
[21] 魏均娴, 王菊芬, 常良宇, 杜远重. 三七的化学研究. 药学学报. 1980,15:359-364.
[22] Liu J H, Wang X, Cai Sh Q, Komatsu K, Namba T. Analysis of the Constituents in the Chinese Drug Notoginseng by Liquid Chromatography-Electrospray MassSpectrometry. J. Chinese Pharm. Sci. 2004,13:225-237.
[23] Manya D M, Juan M C, David W G. Hypoxia-reoxygenation-induced mitochondrial damage and apoptosis in human endothelial cells are inhibited by vitamin C. Free Rad. Bio. & Med. 2005,38(10):1311-1322.
[24] 林蓉,刘俊田,甘伟杰,等. 川芎嗪对缺氧缺糖状态下培养的血管内皮细胞ECV-304 的影响. 中国中药杂志. 2004,29:462-464.
[25] Sunnergren K P, Rovetto M J. Myocyte and endothelial injury with eschemia reperfusion in isolated rat hearts. Am. J. Physiol. 1987,252:1211-1217.
[26] Lindal S, Sorlie D, Jorgensen L. Endothelial cells of the cardiac microvasculature during and after cold cardioplegic ischaemia. Comparison of endothelial and myocyte damage. Scand. J. Thorac. Cardiovasc. Surg. 1988,22:257-265.
[27] 刘立,王友联,张枢. 环孢霉素 A 对局部脑缺血再灌流损伤的改善作用. 中风与神经疾病杂志. 2002,19:106-107.
[28] 邵京山,薛敏,冯玉兰. 复方丹参注射液对脑出血 CT 及脑脊液 LDH 的影响. 实用中西医结合临床. 2002, 2:1-2.
[29] Lobner D. Comparison of the LDH and MTT assays for quantifying cell death; validity for neuronal apoptosis. J. Neuroscience Methods. 2000,96:147-152.
[30] Shuaib A, Sochocka E, Ishaqzayr. Protective effect of hypothermia during ischemia in neutral cell cultures. Neurochem Res. 1993,18:663-665.
[31] Lum H, Barr D A, Shaffer J R, Gordon R J, Ezrin A M, Malik A B. Reoxygenation of endothelial cells increases permeability by oxidant-dependent mechanisms. Circ Res. 1992,70:991-998.
[1] Drasar P., Moravcova. J. Recent advances in analysis of Chinese medical plants and traditional medicines. J. Chromatogr. B. 2004,812:3-21.
[2] Lee MS, Kerns EH. LC-MS applications in drug development. Mass Spectrom. Rev. 1999,18:187-279.
[3] Gough MA, Langley GJ. Analysis of Oilfield Chemicals by Electrospray-Mass Spectrometry. Rapid Commun. Mass Spectrom. 1999,13:227-236.
[4] Ye M., Yan Y. N., Guo D. A. Characterization of phenolic compounds in the Chinese herbal drug Tu-Si-Zi by liquid chromatography coupled to electrospray ionization mass spectrometry. Rapid Commun. Mass Spectrom. 2005,19:1469-1484.
[5] Zhao Y., Chen B., Yao Sh. Zh. Simultaneous determination of abietane-type diterpenes, flavonolignans and phenolic compounds in compound preparations of Silybum marianum and Salvia miltiorrhiza by HPLC-DAD-ESI MS. J. Pharmaceut. Biomed. 2005,38:564-570.
[6] 国家药典委员会. 中华人民共和国药典,一部. 北京:化学工业出版社,2005,p52.
[7] 孔德云. 丹参的化学成分. 中国医药工业杂志,1989,20:279-285.
[8] Lu Y. R., Foo L.Y. Polyphenolics of salvia-a review. Phytochemistry. 2002,59:117-140.
[9] Kasimu R., Tezuka Y., Tanaka K., Gong Zh. N., Li J. X., Basnet P., Namba T., Kadota S. Liquid chromatography-mass spectrometry analysis of diterpenoid constituents of seventeen Salvia plants. J. Trad. Med. 1998,15:109-115.
[10] Kasimu R., Tanaka K., Tezuka Y., Gong Zh. N., Li J. X., Basnet P., Namba T., Kadota S. Comparative study of seventeen Salvia plants: aldose reducatase inhibitory activity of water and MeOH extracts and liquid chromatrgraphy-mass spectrometry (LC-MS) analysis of water extracts. Chem. Pharm. Bull. 1998,46:500-504.
[11] Lin H. C., Chang W. L. Diterpenoids from salvia miltiorrhiza. Phytochemistry. 2000,53:951-953.
[12] Weng X. C., Gordon M. H. Antioxidant Activity of Quinones Extracted from Tanshen (Salvia miltiorrhiza Bunge). J. Agric. Food Chem. 1992,40:1331-1336.
[13] Onitsuku M., Fu J. M., Shinmu N., Maruyama H. B. New platelet aggregation inhibitors from Tan Shen radix of Salvia miltiorrhiza Bunge. Chem. Pharm. Bull.1983,31:1670-1675.
[14] Liu G. T., Zhang T. M., Wang B. E., Wang Y. W. Protective action of seven natural phenolic compounds against peroxidative damage to biomembranes. Biochem. Pharmacol. 1992,43:147-152.
[15] Tang M. K., Ren D. C., Zhang J. T., Du G. H. Effect of salvianolic acids from Salvia miltiorrhiza on regional cerebral blood flow and platelet aggregation in rats. Phytomedicine. 2000,9:405-413.
[16] Chan K., Chui S. H., Wong D. Y. L., Ha W. Y., Chan C. L., Wong R. N. S. Protective effects of Danshensu from the aqueous extract of Salvia miltiorrhiza (danshen) against homocysteine-induced endothelial dysfunction. Life Sci. 2004,75:3157-3171.
[17] Dinga M., Yea T. X., Zhaoa G. R., Yuana Y. J., Guo Z. X. Aqueous extract of Salvia miltiorrhiza attenuates increased endothelial permeability induced by tumor necrosis factor-α. Int. Immunopharmacol. 2005,5:1641-1651.
[18] Li X. Ch., Yu Ch., Cai Y. B., Liu G. Y., Jia J. Y., Wang Y. P. Simultaneous determination of six phenolic constituents of danshen in human serum using liquid chromatography/tandem mass spectrometry. J. Chromatogr. B. 2005,820:41-47.
[19] Zhang J. L., Cui M., He Y., YU H. L., Guo D. A. Chemical fingerprint and metabolic fingerprint analysis of DanShen injection by HPLC-UV and HPLC-MS methods. J. pharmaceut. Biomed. 2005,36:1029-1035.
[20] Tsuda T., Watanabe M., Ohshima K., Yamamoto A., Kawakishi S. and Osawa. T. Antioxidative components isolated from the seed of tamarind (tamarindus indica L.). J. Agric. Food. Chem. 1994,42:2671-2674.
[21] Li L. N. Water soluble active components of Salvia miltiorrhiza and relatedplants. J. Chin. Pharm. Sci. 1997, 6:57-64.
[22] 陈勇,张玲,王世敏. 丹参水溶性成分的电喷雾质谱行为及其特征图谱的初步研究. 分析化学,2004,32(11):1485-1488.
[23] 周长新,罗厚蔚,丹羽正武. 丹参水溶性化学成分的研究. 中国药科大学学报,1999,30(6): 411-416.
[24] 吴志军,欧阳明安,杨崇仁. 大紫丹参的多酚类化合物. 云南植物研究, 1999,21:512-516.
[25] Ai, C. B., Li, L. N., Salvianolic acids D and E: Two new depsides from salvia miltiorrhiza. Planta Med. 1992,58:197-199.
[26] 于文佳,何坚,沈金灿,王小如. 高分辨电喷雾飞行时间质谱测定丹参脂溶性组分. 高等学校化学学报,2003,24(4):621-623.
[27] 薛明,史彦斌,崔颖,张彬,罗永江,周宗田,夏文江,赵荣材. 甘西鼠尾草化学成分的研究. 天然产物研究与开发,1996,12(6):27-32.
[28] M. Battistini, A. Griffini, F. Peterlongo. Identification of tanshinones from Salvia miltiorrhiza roots by liquid chromatography-thermospray mass spectrometry. Fitoterapia. 1995,66(3):265-272.
[29] 丛浦珠. 质谱学在天然有机化学中的应用. 北京:科学出版社,1987:854-860.
[1] Yoshikawa M, Murakami T, Ueno T, Yashiro K, Hirokawa N, Murakami N, Yamahara J, Matsuda H, Saijoh R and Uanaka O. Bioactive saponins and glycosides. VIII. Notoginseng (1) New dammarane-type triterpene oligoglycosides, notoginsenosides-A, -B, -C, and -D, from the dried root of Panax notoginseng. Chem Pharm Bull, 1997, 45: 1039-1045.
[2] Yoshikawa M, Murakami T, Ueno T, Hirokawa N, Yashiro K, Murakami N, Yamahara J, Matsuda H, Saijoh R and Uanaka O. Bioactive saponins and glycosides. IX. Notoginseng (2) Structures of five new dammarane-type triterpene oligoglycosides, notoginsenosides-E, -G, -H, -I and -J, and a novel acetylenic fatty acid glycoside, notoginsenic acidβ-sophoroside, from the dried root of Panax notoginseng. Chem Pharm Bull, 1997, 45: 1056-1062.
[3] Yoshikawa M, Morikawa T, Yashiro K, Murakami T, Matsuda H. Bioactivesaponins and glycosides. XIX. Notoginseng (3) Immunological Adjuvant activity of Notoginsenosides and related saponins: Structures of notoginsenosides-L, -M and –N form the root of Panax notoginseng (Burk.) F. H. Chen. Chem Pharm Bull, 2001, 49: 1452-1456.
[4] 周志华. 三七中皂甙成分分析的研究. 药学学报. 1981,16(7):535-540.
[5] 甘烦远,郑光植. 三七化学成分研究概况. 中国药学杂志. 1992,27(3):138-143.
[6] 李琦, 叶蕴华, 邢其毅. 三七水溶性化学成分及药理研究新进展. 高等学校化学学报. 1996,17:1886-1892.
[7] Zhao P, Liu YQ, Yang CR. Minor dammarane saponins from Panax notoginseng. Phytochemistry. 1996, 41:1419-1422.
[8] Ma WG, Mizutam M, Malterud KE, et al. Saponins from the roots of Panax notoginseng. Phytochemistry. 1999,52:1133-1139.
[9] Li HZ, Teng RW, Yang CR. A novel hexanordammarane glycoside from the roots of Panax notoginseng. Chin Chem Lett. 2001,12:59-62.
[10] 刘刚,鲍建材,郑友兰,张崇禧. 三七的化学成分研究进展. 人参研究 2004,2:10-18.
[11] 郑光植,杨崇仁编. 三七生物学及其应用. 科学技术出版社. 1994,9-18.
[12] 梁宁,赵怀清,周迎春,曲燕,鲁鑫焱,张福蔓. HPLC法同时测定三七总皂苷中 人参皂苷Rg1 和Rb1的含量. 中草药. 2002, 33 (8): 704-705.
[13] Lau AJ, Woo SO, Koh HL. Analysis of saponins in raw and steamed Panax notoginseng using high-performance liquid chromatography with diode array detection. J Chromatogr A, 2003,1011:77-87.
[14] 邸峰,孙毅坤. 高效液相色谱法测定复方丹参片三七皂甙R1的含量. 中国中药杂志. 1996,21:672-673.
[15] Li W, Fitzloff JF. A validated method for quantitative determination of saponins in notoginseng (Panax notoginseng) using high-performance liquid chromatography with evaporative light scattering detection. J Pharm Pharmacol, 2001,53:1637-1643.
[16] 江英桥,王强,马世平,党学东. HPLC-ELSD 及紫外分光光度法测定三七中皂苷的含量. 中草药. 2000,31:737-739.
[17] Bombardelli E, Bonati A, Gabetta B, Martinelli E M, Mustich G. Gas-liquid chromatographic and mass spectrometric investigation on saponins in Panax ginseng extracts. Fitoterapia. 1976,47(3):99-106.
[18] 宋凤瑞,李信林,钱玉民. 系列人参皂甙的快原子轰击质谱研究. 分析化学. 1991,19:924-926.
[19] Park MK, Park JH, Hwang GS, Lee MY, Park IJ. Analysis of ginsenosides by thermospray LC/MS. Koryo Insam Hakhoechi, 1995,19(2):134-137.
[20] Schulten HR, Soldati F. Field desorption mass spectrometry of natural products. Part IX. Identification of ginsenosides from Panax ginseng in fractions obtained by high-performance liquid chromatography by field desorption mass spectrometry, multiple internal reflection infrared spectroscopy and thin-layer chromatography. J. Chromatogr. 1981,212(1):37-49.
[21] 周雨,宋凤瑞,刘淑莹. 人参皂甙的基质辅助激光解吸质谱研究. 化学学报. 1998,56:298-301.
[22] 刘进怀, 王璇, 蔡少青, 小松かつ子, 难波恒雄. 中药三七化学成分的HPLC-ESI-MS分析. 中国药学. 2004, 13:225-237.
[23] Fuzzati N, Cabetta B, Jayakar K. Liquid Chromatography-electrosprary mass spectrometric identification of ginsenosides in Panax ginseng roots. J. Chromatogr. A. 1999,854:69-79.
[24] Ma XQ, Liang XM, Xu Q, Zhang XZ and Xiao HB. Identification of Ginsenosides in Roots of Panax ginseng by HPLC-APCI/MS. Phytochem. Anal. 2005,16:181-187.
[1] Jandera P. Optimisation of gradient elution in normal-phase high-performance liquid chromatography. J. Chromatogr. A. 1998,797:11-22.
[2] Snyder LR, Dolan JW. Initial experiments in HPLC method development: use of a starting gradient. J. Chromatogr. A. 1996,721:3-14.
[3] Heman DP, Billiet HA, Galan LD. Algorithms to correct gradient scan and transfer rule predictions of isocratic retention in RP-HPLC. J. Chromatogr. 1989,463:1-20.
[4] 卢佩章,张玉奎,梁鑫淼. 高效液相色谱及其专家系统. 沈阳:辽宁科学技术出版社. 1992.
[5] 单亦初. 反相高效液相色谱中复杂样品分离条件快速多元优化方法研究. 中国科学院大连化学物理研究所博士论文. 2002.
[6] 王龙星. 中药色谱指纹质量控制基础方法学研究. 中国科学院大连化学物理研究所博士论文. 2003.
[7] 薛兴亚. 复杂未知样品气相色谱分离分析研究理论基础研究. 中国科学院大连化学物理研究所博士论文. 2004.
[8] 徐青. 中药高效液相色谱分析方法发展的理论基础研究. 中国科学院大连化学物理研究所博士论文. 2004.
[9] 李似娇,何大森,何巧红,陈晓敏. 色谱图拟合及重叠峰快速分离解析. 色谱. 1992,10(5):251-254.
[10] 吴宁生,魏伟. EMG 模型中参数的一种关系式. 色谱. 1994,12(1):43.
[11] Baeza-Bazeza JJ, Garcia-Alvarez-Coque MC. Prediction of peak shape as a function of retention in reversed-phase liquid chromatography. J. Chromatogr. A. 2004,1022(1-2):17-24.
[12] 张玉奎,董礼孚,包绵生,周桂敏,林从敬,卢佩章. 分析测试通报. 1984,3:16-19.
[13] 邹汉法. 色谱专家系统的理论基础. 中国科学院大连化学物理研究所博士论文. 1989.
[14] Folley JP, Dorsey JG. A review of the exponentially modified Gaussian (EMG) function: evaluation and subsequent calculation of universal data. J. Chromatogr. Sci. 1984,22(1):40-46.
[15] 马小琼. 人参化学物质基础分析方法研究. 中国科学院大连化学物理研究所博士论文. 2005.
[2] 夏征农. 辞海. 上海:辞书出版社. 1999.
[3] 王付. 经方药物药理临证指南. 北京:中医古籍出版社,1999.
[4] 高晓山. 中药配伍研究问题瞻望. 中国实验方剂学杂志. 1999,5 (6):1-7.
[5] 韩笑, 胡一冰, 邱德文. 中药复方配伍规律研究. 中国中医基础医学杂志.2000,6(8):59-64.
[6] 凌一揆,颜正华. 中药学. 上海:上海科学技术出版社. 1986.
[7] 蒋永光,李认书,金桂花,黎勇. 方剂配伍的研究现状及问题分析. 国医论坛. 2002,17 (1):51-52.
[8] 魏国丽,郑学宝. 方剂配伍规律现代科学内涵研究进展. 中医药临床杂志. 2005,17(5):515-517.
[9] 朱天忠. 芍药甘草汤的临床应用及药理研究. 北京中医杂志. 1985,4(4):36-40.
[10] 鲍军. 泻心汤体外抗凝及抗血小板聚集作用的研究. 中成药研究. 1988,(10):28-30.
[11] Yuan D, Komatsu K, Cui Z, Kano Y. Pharmacological properties oftraditional medicines (XXVII). Interaction between ephedra herb and gypsum under hyperthermal conditions in rats. Biol. Pharm. Bull. 2002, 25(7):872-874.
[12] 范颖. 试论中医方剂配伍规律研究的现状与基本思路. 江苏中医药. 2003,24 (4):9-11.
[13] 韩笑,胡一冰,邱德文. 中药复方配伍规律研究. 中国中医基础医学杂志. 2000,6(8):59-64.
[14] 关怀,穆阳,高燕,许艳丽,苏宏宇. 不同配伍剂量对大黄-黄连药对的有效成分及药理作用的影响研究. 北京中医. 2000,2:53-55.
[15] 顿宝生,许爱英. 中医方剂配伍规律的探析. 陕西中医学院学报. 2000,23(6):4-5.
[16] 聂淑琴,杨庆,李兰芳,黄璐琦. 柴胡与赤芍、醋柴胡与白芍配伍前后药效学比较研究. 中国实验方剂学杂志. 2002,8(3):11-14.
[17] 赵建荣, 李晓玫. 中药复方物质基础研究的现状与进展. 中草药. 2003,34(11):963-967.
[18] 吴立军主编. 中药化学. 北京:中国医药科技出版社. 2000.
[19] 陈耀祖. 中药复方化学研究策略诹议. 化学进展. 1999,11(2):188-192.
[20] 刘建利. 中药复方化学研究的思路. 中成药,1992,14(10):49-53.
[21] 何祥久,邱峰,姚新生. 中药复方研究现状和思路. 化学进展. 2001,13(6):481-485.
[22] 汪显阳. 大黄牡丹汤不同配伍对有效成分煎出的影响. 中国中医药科技. 2002,9(3):161-162.
[23] 何伟,王宁,秦林,张翔. 川乌与白芍配伍前后乌头碱和芍药苷煎出量的测定. 中国药学杂志. 2002,37(9):680-683.
[24] 何伟,秦林,司淑媛. 附子与白芍配伍前后乌头碱煎出量的测定. 中草药.2002,33(7):600-602.
[25] 段天璇,马长华,侯艳霞,刘舒萍,陈韬,伦丽辉,小熊亮子,李宇航. 半夏泻心汤不同配伍情况下部分化学成分变化. 中国中药杂志. 2002,27(2):363-365.
[26] 李吉来,陈飞龙,刘传明,罗佳波. 麻黄汤中麻黄碱的 GC-MS 法测定及配伍因素对汤剂中该成分含量的影响. 中草药. 2002,33(4):703-705.
[27] 陈建萍,谭炳炎,吴伟康. 四逆汤中附子甘草配伍规律研究. 中国实验方剂杂志. 2001, 7 (3) ∶16-20.
[28] 肖红斌. 中药复方液相色谱统一方法的研究及其在补阳还伍汤中的应用. 中国科学院大连化学物理研究所博士论文. 1997.
[29] 张子忠. 中药质量控制方法及其配伍复方中化学成分变化规律的应用基础研究. 中国科学院大连化学物理研究所博士论文. 2000.
[30] 李秀玲. 精制血府逐瘀复方配伍的药效物质基础研究. 中国科学院大连化学物理研究所博士论文. 2003.
[31] 朱丹妮,李志明,严永清. 生脉散复方化学的动态变化与药效关系的研究-生脉散复方化学的研究(Ⅱ). 中国中药杂志. 1998,23(5):230-232.
[32] 朱丹妮,李志明,严永清. 生脉散复方化学动态变化与药效关系的研究-生脉散复方化学的研究(Ⅲ). 中国中药杂志. 1998,23(8):291-293
[33] 刘建勋. 中药药效学研究与中医临床相结合. 中药新药与临床药理. 1997,8(3):137-138.
[34] 严永清. 关于加强中药复方化学成分的研究. 中国中药杂志. 1985,10(2):3-5.
[35] 严永清,马晓红,李志明. 生脉散复方化学成分的动态变化与药效的研究(三). 中国学术期刊文摘(科技快报). 1998,4(7):860-862.
[36] 严永清,朱丹妮,李志明. 生脉散复方化学成分的动态变化与药效的研究(五). 中国学术期刊文摘(科技快报). 1998,4(7):865-868.
[37] 宋小莉,高艳青,牛欣,司银楚. 复方配伍实验设计方法评述. 中西医结合学报. 2003,1(3):177-179.
[38] 王昕. 大黄 虫丸的研究及应用概况. 中成药. 1997,19(2):45-46.
[39] 孙洋,陈婷,徐强. 从药对的角度考察复方配伍规律. 世界科学技术-中医药现代化研究. 2004,6(1):17-21.
[40] 吴建新,严永清,蒋莹. 六味地黄汤的化学研究. 现代应用药学. 1990,7(1):16-18.
[41] 蒋莹,赵陆华,严永清. 六味地黄汤及其配伍对过氧化脂质及脂褐质含量的影响. 中国中药杂志. 1991,16(3):175-176.
[42] 宋宗华,戴舒佳,黎辉琴. 苓桂术甘汤配伍机制研究. 中国中药杂志. 2002,27(10):760-762.
[43] 王庆国,李宇航,赵琰. 半夏泻心汤及其拆方对正常大鼠胃肠运动功能的影响. 北京中医药大学学报. 2001,24(6):19-21.
[44] 曹华,何国,李政木. 经方“药对”配伍规律研究的现状及分析. 国医论坛. 2003,18(4):1-3.
[45] 姚新生,胡柯. 中药复方现代化研究. 化学进展. 1999,19(2):192-196.
[46] 袁久荣. 关于方剂学科发展中若干前沿关键问题的研究. 中国中医基础医学杂志. 2005,11(9):681-685.
[47] 余林中,罗佳波,谭晓梅. 方剂配伍规律研究应以物质基础为核心. 中国中医基础医学杂志. 1999, 5(10):18-20.
[48] 国家药典委员会. 中华人民共和国药典,一部. 北京:化学工业出版社,2005
[49] 徐任生主编. 丹参-生物学及其应用. 北京:科学出版社,1990.
[50] 郑虎占,董泽宏,余靖. 中药现代研究与应用(第2卷). 北京:学苑出版社. 庶虫1997,P1093.
[51] 郑光植,杨崇仁编. 三七-生物学及其应用. 北京:科学出版社,1994.
[52] 郑虎占,董泽宏,余靖. 中药现代研究与应用(第 1 卷). 北京:学苑出版社. 1997,P195.
[53] 陈文莉. 三七配伍应用探析. 江西中医药. 2002,33(2):48.
[54] 中国医学科学院药物研究所编著. 中草药现代研究(第2卷). 北京:北京医科大学中国协和医科大学联合出版社. 1996,472-479.
[55] 孔德云. 丹参的化学成分. 中国医药工业杂志. 1989,20(6):279-285.
[56] 郑国墀,柿泽宽. 丹参化学成分的研究概况. 中国药学杂志. 1989,24(1):5-10.
[57] 周长新,罗厚蔚,丹羽正武. 丹参水溶性化学成分的研究. 中国药科大学学报,1999, 30(6): 411-416.
[58] Lu YR, Foo LY. Polyphenolics of salvia-a review. Phytochemistry. 2002, 59,117-140.
[59] Kasimu R, Tezuka Y, Tanaka K, Gong ZN, Li JX, Basnet P, Namba T, Kadota S. Liquid chromatography-mass spectrometry analysis of diterpenoid constituents of seventeen Salvia plants. J. Trad. Med. 1998, 15, 109-115.
[60] Kasimu R, Tanaka K, Tezuka Y, Gong ZN, Li JX, Basnet P, Namba T, Kadota S. Comparative study of seventeen Salvia plants: aldose reducatase inhibitory activity of water and MeOH extracts and liquid chromatrgraphy-mass spectrometry (LC-MS) analysis of water extracts. Chem. Pharm. Bull. 1998, 46, 500-504.
[61] Lin HC, Chang WL. Diterpenoids from salvia miltiorrhiza. Phytochemistry. 2000, 53, 951-953.
[62] Zhang JL, Cui M, He Y, YU HL, Guo DA. Chemical fingerprint and metabolicfingerprint analysis of DanShen injection by HPLC-UV and HPLC-MS methods. J. Pharmaceut. Biomed. 2005, 36, 1029-1035.
[63] Tsuda T, Watanabe M, Ohshima K, Yamamoto A, Kawakishi S. and Osawa. T. Antioxidative components isolated from the seed of tamarind (tamarindus indica L.). J. Agric. Food. Chem. 1994, 42, 2671-2674.
[64] 张建虎,叶芊. 丹参酮药理作用. 中华医学研究杂志. 2005,5(6):51-53.
[65] 陈维洲. 丹参的药理. 药学学报. 1984,19(11):876-880.
[66] Fung KP,Zeng LH,Wu J,Wong HN,Lee CM, Hon PM,Chang HM,Wu TW. Demonstration of the myocardial salvage effect of lithospermic acid B isolated from the aqueous extract of Salvia miltiorrhiza. Life Sci. 1993,52(22):1239-44.
[67] Wu TW,Zeng LH,Fung KP,Wu J,Pang H,Grey AA,Weisel RD,Wang JY. Effect of sodium tanshinone IIA sulfonate in the rabbit myocardium and on human cardiomyocytes and vascular endothelial cells. Biochem Pharmacol. 1993,46(12):2327-2332.
[68] Xin CW. and Michael HG. Antioxidant Activity of Quinones Extracted from Tanshen (Salvia miltiorrhiza Bunge). J. Agric. Food. Chem. 1992,40:1331-1336.
[69] Liu J,Shen HM,Ong CN. Role of intracellar thiol depletion, mitochondrial dysfunction and reactiove oxygen species in Salvia Miltiorrhiza-induced apoptosiss in human hepatoma HepG2 cells. Life Sciences. 2001,69:1833-1850
[70] 中国医学科学院药物研究所编著. 中草药现代研究(第 3 卷). 北京:北京医科大学中国协和医科大学联合出版社. 1996,191-192.
[71] 甘烦远,郑光植. 三七化学成分研究概况. 中国药学杂志. 1992,27(3):138-143.
[72] Yoshikawa M, Murakami T, Ueno T, Yashiro K, Hirokawa N, Murakami N, Yamahara J, Matsuda H, Saijoh R and Uanaka O. Bioactive saponins and glycosides. VIII. Notoginseng (1) New dammarane-type triterpene oligoglycosides, notoginsenosides-A, -B, -C, and -D, from the dried root of Panax notoginseng. Chem Pharm Bull, 1997, 45: 1039-1045.
[73] Yoshikawa M, Murakami T, Ueno T, Hirokawa N, Yashiro K, Murakami N, Yamahara J, Matsuda H, Saijoh R and Uanaka O. Bioactive saponins and glycosides. IX. Notoginseng (2) Structures of five new dammarane-type triterpene oligoglycosides, notoginsenosides-E, -G, -H, -I and -J, and a novel acetylenic fatty acid glycoside, notoginsenic acidβ-sophoroside, from the dried root of Panax notoginseng. Chem Pharm Bull, 1997, 45: 1056-1062.
[74] Yoshikawa M, Morikawa T, Yashiro K, Murakami T, Matsuda H. Bioactive saponins and glycosides. XIX. Notoginseng (3) Immunological Adjuvant activity of Notoginsenosides and related saponins: Structures of notoginsenosides-L, -M and –N form the root of Panax notoginseng (Burk.) F. H. Chen. Chem Pharm Bull, 2001, 49: 1452-1456.
[75] 魏均娴,王菊芬. 三七叶黄酮类成分的研究. 中药通报. 1987,12(1):31-33
[76] Ma WG, Mizutam M, Malterud KE, et al. Saponins from the roots of Panax notoginseng. Phytochemistry. 1999,52:1133-1139.
[77] 杨柳,叶蕴华,邢其毅. 人参中γ-氨基丁酸的分离、鉴定及氨基酸的定量分析. 科学通报. 1991,(7):513-518.
[78] Liu JH,Wang X,Cai SQ,Komatsu K and Namba T. Analysis of the Constituents in the Chinese Drug Notoginseng by Liquid Chromatography-Electrospray Mass Spectrometry. J. Chin. Pharm. Sci. 2004,13(4):225-237.
[79] 李琦. 三七水溶性化学成分及其药理研究新近展. 高等学校化学学报. 1995,17(12):1886-1892.
[80] 蔡燕玲,黎罕文. 三七的药理研究进展. 陕西中医学院学报. 2001,24(5):57-58.
[81] Sun HX, Ye YP, Pan HJ, Pan YJ. Adjuvant effect of Panax notoginseng saponins on the immune responses to ovalbumin in mice. Vaccine. 2004,22:3882–3889.
[82] Li LX, Wang ZC,Huang ZH,Zhang YM and Wei JX. Effects of Panax notoginseng saponins on acute cerebral ischemia. Chin. Pharmacol. Bull. 1991,7:56-59.
[83] Huang C,Gu TX,Guan YY,and Wang JQ. Effects of Panax notoginseng saponins on myocardial ischemia and reperfusion injury in conscious rabbit. Chin. Pharmacol. Bull. 1991,7:190-193.
[84] 刘小清,罗宪,欧阳小安. 复方丹参剂型研究简况. 时珍国医国药. 2001,12(5):474-475.
[85] 罗晓健,毕开顺,周书余. 复方丹参片的研究概况. 中成药. 2001,23(5):371-374.
[86] 王筠默,李仪奎,张海根. 复方丹参片的药理作用研究.中成药研究. 1984,(11):22-24.
[87] 张昱,王绵之,黄启福. 复方丹参片对大鼠心肌缺血再灌注损伤的保护作用. 河南中医刊. 1997,12 (6):15-17.
[88] 吴乃峰,阎希军,朱永宏. 复方丹参滴丸与复方丹参片的药理作用比较. 中成药. 1993,15 (8):24-25.
[89] 刘婷,秦彩玲,张毅,康立源,孙有富,张伯礼. 丹参、三七不同比例配伍对正常家兔血小板聚集性、粘附性的影响. 中国中药杂志. 2002,27(8):609-611.
[90] 商洪才,张伯礼,高秀梅,王怡,李玉红,康立源,杜嵘,江海涛. 丹参三七药对不同配比药效学比较研究. 辽宁中医杂志. 2002,29(5):297-299.
[91] Olesze WA. Chromatographic determination of plant saponins. J. Chromatogr. A. 2002,967:147-162.
[92] Caniova A. and Brandsteterova E. HPLC analysis of phenolic acids in plant material. Chem. Anal.(warsaw). 2001,46:757-780.
[93] Drasar P., Moravcova. J. Recent advances in analysis of Chinese medical plants and traditional medicines. J. Chromatogr. B. 2004, 812:3-21
[94] Yao LH, Jiang YM, Datta N, Singanusong R, Liu X, Duan J, Raymont K, Lisle A, Xu Y. HPLC analyses of flavanols and phenolic acids in the fresh young shoots of tea (Camellia sinensis) grown in Australia. Food Chemistry. 2004,84:253-263.
[95] Li L, Zhang JL, Sheng YX, Guo DA, Wang Q and Guo HZ. Simultaneous quantification of six major active saponins of Panax notoginseng by high-performance liquid chromatography-UV method. J. Pharmaceut. Biomed. 2005,38:45-51.
[96] Lazarowych N.J. and Pekos P. Use of Fingerprinting and marker compounds for inentification and standardiazation of botanical drugs: strategies for applying pharmaceutical HPLC analysis to herbal products. Drug Inf. J. 1998, 32:497-512.
[97] Lu GH, Chan K, Liang YZ, Leung K, Chan CL, Jiang ZH, Zhao ZZ. Development of high-performance liquid chromatographic fingerprints for distinguishing Chinese Angelica from related umbelliferae herbs. JChromatogra. A. 2005,1073:383-392.
[98] Lee MS, Kerns EH. LC-MS applications in drug development. Mass Spectrom. Rev. 1999,18:187-279.
[99] Ye M., Yan Y. N., Guo D. A. Characterization of phenolic compounds in the Chinese herbal drug Tu-Si-Zi by liquid chromatography coupled to electrospray ionization mass spectrometry. Rapid Commun. Mass Spectrom. 2005, 19:1469-1484.
[100] 李秀玲,肖红斌,胡皆汉,梁鑫淼. 液相色谱/质谱/质谱联用鉴定芍药中的一种新化合物. 分析化学. 2003,31(3):329-331.
[101] 王莉,肖红斌,胡皆汉,王加宁,梁鑫淼. 天麻中对羟基苯类化合物的高效液相色谱-质谱研究. 分析化学. 2003,31(8):954-957.
[102] Xiaozhe Zhang, Hongbin Xiao, Qing Xu, Xiuling Li, Jianning Wang, Xinmiao Liang. Characterization of Phthalides in Ligusticum chuanxiong by Liquid Chromatographic-Atmospheric Pressure Chemical Ionization-Mass Spectrometry. J. Chromatogr. Sci. 2003,41:428-433.
[103] Ma XQ, Liang XM, Xu Q, Zhang XZ and Xiao HB. Identification of Ginsenosides in Roots of Panax ginseng by HPLC-APCI/MS. Phytochem. Anal. 2005,16:181-187.
[1] 黄泰康. 中药系统工程体系的建立与发展初探. 科学通报, 1995,40(19):1729-1734.
[2] 阴键,郭力弓主编. 中药现代研究与临床应用,北京:学苑出版社,1993.
[3] 黄泰康主编. 常用中药成分与药理研究,北京:中国医药科技出版社,1994
[4] 刘建利. 中药复方化学研究的思路. 中成药,1992,14(10):49-53.
[5] 王静蓉,马终瑜,严永清等. 芍药甘草配伍化学变化研究. 时珍国医国药,2000,11(2):102-104.
[6] 汪显阳. 大黄牡丹汤不同配伍对有效成分煎出的影响. 中国中医药科技,2002,9(3):161-162.
[7] 何伟,秦林,司淑媛. 附子与白芍配伍前后乌头碱煎出量的测定. 中草药,2002,33(7):600-602.
[8] 陈建萍,谭炳炎,吴伟康,张敏生,李礼娟,韩超. 四逆汤中附子甘草配伍规律研究. 中国实验方剂学杂志,2001,7(3):18-19.
[9] 夏云,李志明,朱丹妮. 生脉散复方化学动态变化与药效关系的研究-生脉散复方化学的研究(Ⅰ).中国中药杂志,1998,23(4):230-232.
[10] 朱丹妮,李志明,严永清. 生脉散复方化学动态变化与药效关系的研究-生脉散复方化学的研究(Ⅱ).中国中药杂志,1998,23(5):291-293.
[11] 朱丹妮,严永清,李志明. 生脉散复方化学动态变化与药效关系的研究-生脉散复方化学的研究(Ⅲ).中国中药杂志,1998,23(8):483-485.
[12] 肖红斌. 中药复方液相色谱统一方法的研究及其在补阳还伍汤中的应用. 中国科学院大连化学物理研究所博士论文,1997.
[13] 张子忠. 中药质量控制方法及其配伍复方中化学成分变化规律的应用基础研究. 中国科学院大连化学物理研究所博士论文,2000.
[14] 李秀玲. 精制血府逐瘀复方配伍的药效物质基础研究. 中国科学院大连化学物理研究所博士论文,2003.
[1] 李庭凯,王晓春,赵勤萍,等. 复方丹参滴丸治疗冠心病心绞痛心血瘀阻证的临床研究. 中西医结合心脑血管病杂志, 2003,1:215-216.
[2] 秦培森. 复方丹参滴丸治疗冠状动脉粥样硬化性心脏病心绞痛临床观察. 中成药. 2000,22:630-632.
[3] 林萍,陈贤岸. 复方丹参片与复方丹参滴丸治疗心绞痛疗效比较及原因分析. 广州医药,2001,32:73.
[4] 杨庆福,李彩玲,陈俭. 复方丹参滴丸对冠心病心绞痛患者血管内皮功能的影响. 新乡医学院学报,2003,20:127-128.
[5] 国家药典委员会. 中华人民共和国药典. 一部. 北京:化学工业出版社. 2005,527-528.
[6] 戈延茹,方亮,李杰. 复方丹参片药典方制剂的研究. 中成药. 2002, 24:872-875.
[7] 王作顺. 复方丹参滴丸的研究进展. 吉林中医药. 2003,23:46-48.
[8] 余世春,琚小龙,段广勋. 丹参的化学成分和药理活性研究概况. 安徽卫生职业技术学院学报,2002,1:43-47.
[9] 丁章森. 丹参的临床药理研究概况. 湖北中医学院学报. 2000,12:56-59.
[10] 徐勇. 丹参对心血管系统的作用及其临床应用. 天津中医学院学报. 1998,17:31.
[11] 杨志刚,陈阿琴,俞颂东. 三七药理研究新进展. 上海中医药杂志. 2005,39:59-62.
[12] 李琦. 三七水溶性化学成分及及药理研究新进展. 高等学校化学学报. 1995,17:1886-1892.
[13] 韩金安, 胡威夷. 三七总皂甙对缺血性脑损伤保护作用的研究进展. 中国中西医结合杂志. 1996,16:506-507.
[14] 陈云红, 施鹤高, 王玉梅. 近年来三七的药理研究进展. 中草药. 1995,26:160-162.
[15] 陈泉生,郑振源,杨士琰,陈古荣. 三七(须根)总皂甙的药理研究. 中药通报. 1987,12:45-47.
[16] 费震宇, 王文健,陈响中. 三七用于冠心病治疗的药理研究.时珍国药研究. 1997,8:17-18.
[17] 孔德云. 丹参的化学成分. 中国医药工业杂志. 1989,20:279-285.
[18] 郑国樨,柿泽宽. 丹参化学成分的研究概况. 中国药学杂志. 1989,24:5-10.
[19] Lu Y-R, Foo LY. Polyphenolics of salvia-a review. Phytochemistry. 2002,59:117-140.
[20] 刘刚,鲍建材,郑友兰,张崇禧. 三七的化学成分研究进展. 人参研究. 2004,2:10-18.
[21] 魏均娴, 王菊芬, 常良宇, 杜远重. 三七的化学研究. 药学学报. 1980,15:359-364.
[22] Liu J H, Wang X, Cai Sh Q, Komatsu K, Namba T. Analysis of the Constituents in the Chinese Drug Notoginseng by Liquid Chromatography-Electrospray MassSpectrometry. J. Chinese Pharm. Sci. 2004,13:225-237.
[23] Manya D M, Juan M C, David W G. Hypoxia-reoxygenation-induced mitochondrial damage and apoptosis in human endothelial cells are inhibited by vitamin C. Free Rad. Bio. & Med. 2005,38(10):1311-1322.
[24] 林蓉,刘俊田,甘伟杰,等. 川芎嗪对缺氧缺糖状态下培养的血管内皮细胞ECV-304 的影响. 中国中药杂志. 2004,29:462-464.
[25] Sunnergren K P, Rovetto M J. Myocyte and endothelial injury with eschemia reperfusion in isolated rat hearts. Am. J. Physiol. 1987,252:1211-1217.
[26] Lindal S, Sorlie D, Jorgensen L. Endothelial cells of the cardiac microvasculature during and after cold cardioplegic ischaemia. Comparison of endothelial and myocyte damage. Scand. J. Thorac. Cardiovasc. Surg. 1988,22:257-265.
[27] 刘立,王友联,张枢. 环孢霉素 A 对局部脑缺血再灌流损伤的改善作用. 中风与神经疾病杂志. 2002,19:106-107.
[28] 邵京山,薛敏,冯玉兰. 复方丹参注射液对脑出血 CT 及脑脊液 LDH 的影响. 实用中西医结合临床. 2002, 2:1-2.
[29] Lobner D. Comparison of the LDH and MTT assays for quantifying cell death; validity for neuronal apoptosis. J. Neuroscience Methods. 2000,96:147-152.
[30] Shuaib A, Sochocka E, Ishaqzayr. Protective effect of hypothermia during ischemia in neutral cell cultures. Neurochem Res. 1993,18:663-665.
[31] Lum H, Barr D A, Shaffer J R, Gordon R J, Ezrin A M, Malik A B. Reoxygenation of endothelial cells increases permeability by oxidant-dependent mechanisms. Circ Res. 1992,70:991-998.
[1] Drasar P., Moravcova. J. Recent advances in analysis of Chinese medical plants and traditional medicines. J. Chromatogr. B. 2004,812:3-21.
[2] Lee MS, Kerns EH. LC-MS applications in drug development. Mass Spectrom. Rev. 1999,18:187-279.
[3] Gough MA, Langley GJ. Analysis of Oilfield Chemicals by Electrospray-Mass Spectrometry. Rapid Commun. Mass Spectrom. 1999,13:227-236.
[4] Ye M., Yan Y. N., Guo D. A. Characterization of phenolic compounds in the Chinese herbal drug Tu-Si-Zi by liquid chromatography coupled to electrospray ionization mass spectrometry. Rapid Commun. Mass Spectrom. 2005,19:1469-1484.
[5] Zhao Y., Chen B., Yao Sh. Zh. Simultaneous determination of abietane-type diterpenes, flavonolignans and phenolic compounds in compound preparations of Silybum marianum and Salvia miltiorrhiza by HPLC-DAD-ESI MS. J. Pharmaceut. Biomed. 2005,38:564-570.
[6] 国家药典委员会. 中华人民共和国药典,一部. 北京:化学工业出版社,2005,p52.
[7] 孔德云. 丹参的化学成分. 中国医药工业杂志,1989,20:279-285.
[8] Lu Y. R., Foo L.Y. Polyphenolics of salvia-a review. Phytochemistry. 2002,59:117-140.
[9] Kasimu R., Tezuka Y., Tanaka K., Gong Zh. N., Li J. X., Basnet P., Namba T., Kadota S. Liquid chromatography-mass spectrometry analysis of diterpenoid constituents of seventeen Salvia plants. J. Trad. Med. 1998,15:109-115.
[10] Kasimu R., Tanaka K., Tezuka Y., Gong Zh. N., Li J. X., Basnet P., Namba T., Kadota S. Comparative study of seventeen Salvia plants: aldose reducatase inhibitory activity of water and MeOH extracts and liquid chromatrgraphy-mass spectrometry (LC-MS) analysis of water extracts. Chem. Pharm. Bull. 1998,46:500-504.
[11] Lin H. C., Chang W. L. Diterpenoids from salvia miltiorrhiza. Phytochemistry. 2000,53:951-953.
[12] Weng X. C., Gordon M. H. Antioxidant Activity of Quinones Extracted from Tanshen (Salvia miltiorrhiza Bunge). J. Agric. Food Chem. 1992,40:1331-1336.
[13] Onitsuku M., Fu J. M., Shinmu N., Maruyama H. B. New platelet aggregation inhibitors from Tan Shen radix of Salvia miltiorrhiza Bunge. Chem. Pharm. Bull.1983,31:1670-1675.
[14] Liu G. T., Zhang T. M., Wang B. E., Wang Y. W. Protective action of seven natural phenolic compounds against peroxidative damage to biomembranes. Biochem. Pharmacol. 1992,43:147-152.
[15] Tang M. K., Ren D. C., Zhang J. T., Du G. H. Effect of salvianolic acids from Salvia miltiorrhiza on regional cerebral blood flow and platelet aggregation in rats. Phytomedicine. 2000,9:405-413.
[16] Chan K., Chui S. H., Wong D. Y. L., Ha W. Y., Chan C. L., Wong R. N. S. Protective effects of Danshensu from the aqueous extract of Salvia miltiorrhiza (danshen) against homocysteine-induced endothelial dysfunction. Life Sci. 2004,75:3157-3171.
[17] Dinga M., Yea T. X., Zhaoa G. R., Yuana Y. J., Guo Z. X. Aqueous extract of Salvia miltiorrhiza attenuates increased endothelial permeability induced by tumor necrosis factor-α. Int. Immunopharmacol. 2005,5:1641-1651.
[18] Li X. Ch., Yu Ch., Cai Y. B., Liu G. Y., Jia J. Y., Wang Y. P. Simultaneous determination of six phenolic constituents of danshen in human serum using liquid chromatography/tandem mass spectrometry. J. Chromatogr. B. 2005,820:41-47.
[19] Zhang J. L., Cui M., He Y., YU H. L., Guo D. A. Chemical fingerprint and metabolic fingerprint analysis of DanShen injection by HPLC-UV and HPLC-MS methods. J. pharmaceut. Biomed. 2005,36:1029-1035.
[20] Tsuda T., Watanabe M., Ohshima K., Yamamoto A., Kawakishi S. and Osawa. T. Antioxidative components isolated from the seed of tamarind (tamarindus indica L.). J. Agric. Food. Chem. 1994,42:2671-2674.
[21] Li L. N. Water soluble active components of Salvia miltiorrhiza and relatedplants. J. Chin. Pharm. Sci. 1997, 6:57-64.
[22] 陈勇,张玲,王世敏. 丹参水溶性成分的电喷雾质谱行为及其特征图谱的初步研究. 分析化学,2004,32(11):1485-1488.
[23] 周长新,罗厚蔚,丹羽正武. 丹参水溶性化学成分的研究. 中国药科大学学报,1999,30(6): 411-416.
[24] 吴志军,欧阳明安,杨崇仁. 大紫丹参的多酚类化合物. 云南植物研究, 1999,21:512-516.
[25] Ai, C. B., Li, L. N., Salvianolic acids D and E: Two new depsides from salvia miltiorrhiza. Planta Med. 1992,58:197-199.
[26] 于文佳,何坚,沈金灿,王小如. 高分辨电喷雾飞行时间质谱测定丹参脂溶性组分. 高等学校化学学报,2003,24(4):621-623.
[27] 薛明,史彦斌,崔颖,张彬,罗永江,周宗田,夏文江,赵荣材. 甘西鼠尾草化学成分的研究. 天然产物研究与开发,1996,12(6):27-32.
[28] M. Battistini, A. Griffini, F. Peterlongo. Identification of tanshinones from Salvia miltiorrhiza roots by liquid chromatography-thermospray mass spectrometry. Fitoterapia. 1995,66(3):265-272.
[29] 丛浦珠. 质谱学在天然有机化学中的应用. 北京:科学出版社,1987:854-860.
[1] Yoshikawa M, Murakami T, Ueno T, Yashiro K, Hirokawa N, Murakami N, Yamahara J, Matsuda H, Saijoh R and Uanaka O. Bioactive saponins and glycosides. VIII. Notoginseng (1) New dammarane-type triterpene oligoglycosides, notoginsenosides-A, -B, -C, and -D, from the dried root of Panax notoginseng. Chem Pharm Bull, 1997, 45: 1039-1045.
[2] Yoshikawa M, Murakami T, Ueno T, Hirokawa N, Yashiro K, Murakami N, Yamahara J, Matsuda H, Saijoh R and Uanaka O. Bioactive saponins and glycosides. IX. Notoginseng (2) Structures of five new dammarane-type triterpene oligoglycosides, notoginsenosides-E, -G, -H, -I and -J, and a novel acetylenic fatty acid glycoside, notoginsenic acidβ-sophoroside, from the dried root of Panax notoginseng. Chem Pharm Bull, 1997, 45: 1056-1062.
[3] Yoshikawa M, Morikawa T, Yashiro K, Murakami T, Matsuda H. Bioactivesaponins and glycosides. XIX. Notoginseng (3) Immunological Adjuvant activity of Notoginsenosides and related saponins: Structures of notoginsenosides-L, -M and –N form the root of Panax notoginseng (Burk.) F. H. Chen. Chem Pharm Bull, 2001, 49: 1452-1456.
[4] 周志华. 三七中皂甙成分分析的研究. 药学学报. 1981,16(7):535-540.
[5] 甘烦远,郑光植. 三七化学成分研究概况. 中国药学杂志. 1992,27(3):138-143.
[6] 李琦, 叶蕴华, 邢其毅. 三七水溶性化学成分及药理研究新进展. 高等学校化学学报. 1996,17:1886-1892.
[7] Zhao P, Liu YQ, Yang CR. Minor dammarane saponins from Panax notoginseng. Phytochemistry. 1996, 41:1419-1422.
[8] Ma WG, Mizutam M, Malterud KE, et al. Saponins from the roots of Panax notoginseng. Phytochemistry. 1999,52:1133-1139.
[9] Li HZ, Teng RW, Yang CR. A novel hexanordammarane glycoside from the roots of Panax notoginseng. Chin Chem Lett. 2001,12:59-62.
[10] 刘刚,鲍建材,郑友兰,张崇禧. 三七的化学成分研究进展. 人参研究 2004,2:10-18.
[11] 郑光植,杨崇仁编. 三七生物学及其应用. 科学技术出版社. 1994,9-18.
[12] 梁宁,赵怀清,周迎春,曲燕,鲁鑫焱,张福蔓. HPLC法同时测定三七总皂苷中 人参皂苷Rg1 和Rb1的含量. 中草药. 2002, 33 (8): 704-705.
[13] Lau AJ, Woo SO, Koh HL. Analysis of saponins in raw and steamed Panax notoginseng using high-performance liquid chromatography with diode array detection. J Chromatogr A, 2003,1011:77-87.
[14] 邸峰,孙毅坤. 高效液相色谱法测定复方丹参片三七皂甙R1的含量. 中国中药杂志. 1996,21:672-673.
[15] Li W, Fitzloff JF. A validated method for quantitative determination of saponins in notoginseng (Panax notoginseng) using high-performance liquid chromatography with evaporative light scattering detection. J Pharm Pharmacol, 2001,53:1637-1643.
[16] 江英桥,王强,马世平,党学东. HPLC-ELSD 及紫外分光光度法测定三七中皂苷的含量. 中草药. 2000,31:737-739.
[17] Bombardelli E, Bonati A, Gabetta B, Martinelli E M, Mustich G. Gas-liquid chromatographic and mass spectrometric investigation on saponins in Panax ginseng extracts. Fitoterapia. 1976,47(3):99-106.
[18] 宋凤瑞,李信林,钱玉民. 系列人参皂甙的快原子轰击质谱研究. 分析化学. 1991,19:924-926.
[19] Park MK, Park JH, Hwang GS, Lee MY, Park IJ. Analysis of ginsenosides by thermospray LC/MS. Koryo Insam Hakhoechi, 1995,19(2):134-137.
[20] Schulten HR, Soldati F. Field desorption mass spectrometry of natural products. Part IX. Identification of ginsenosides from Panax ginseng in fractions obtained by high-performance liquid chromatography by field desorption mass spectrometry, multiple internal reflection infrared spectroscopy and thin-layer chromatography. J. Chromatogr. 1981,212(1):37-49.
[21] 周雨,宋凤瑞,刘淑莹. 人参皂甙的基质辅助激光解吸质谱研究. 化学学报. 1998,56:298-301.
[22] 刘进怀, 王璇, 蔡少青, 小松かつ子, 难波恒雄. 中药三七化学成分的HPLC-ESI-MS分析. 中国药学. 2004, 13:225-237.
[23] Fuzzati N, Cabetta B, Jayakar K. Liquid Chromatography-electrosprary mass spectrometric identification of ginsenosides in Panax ginseng roots. J. Chromatogr. A. 1999,854:69-79.
[24] Ma XQ, Liang XM, Xu Q, Zhang XZ and Xiao HB. Identification of Ginsenosides in Roots of Panax ginseng by HPLC-APCI/MS. Phytochem. Anal. 2005,16:181-187.
[1] Jandera P. Optimisation of gradient elution in normal-phase high-performance liquid chromatography. J. Chromatogr. A. 1998,797:11-22.
[2] Snyder LR, Dolan JW. Initial experiments in HPLC method development: use of a starting gradient. J. Chromatogr. A. 1996,721:3-14.
[3] Heman DP, Billiet HA, Galan LD. Algorithms to correct gradient scan and transfer rule predictions of isocratic retention in RP-HPLC. J. Chromatogr. 1989,463:1-20.
[4] 卢佩章,张玉奎,梁鑫淼. 高效液相色谱及其专家系统. 沈阳:辽宁科学技术出版社. 1992.
[5] 单亦初. 反相高效液相色谱中复杂样品分离条件快速多元优化方法研究. 中国科学院大连化学物理研究所博士论文. 2002.
[6] 王龙星. 中药色谱指纹质量控制基础方法学研究. 中国科学院大连化学物理研究所博士论文. 2003.
[7] 薛兴亚. 复杂未知样品气相色谱分离分析研究理论基础研究. 中国科学院大连化学物理研究所博士论文. 2004.
[8] 徐青. 中药高效液相色谱分析方法发展的理论基础研究. 中国科学院大连化学物理研究所博士论文. 2004.
[9] 李似娇,何大森,何巧红,陈晓敏. 色谱图拟合及重叠峰快速分离解析. 色谱. 1992,10(5):251-254.
[10] 吴宁生,魏伟. EMG 模型中参数的一种关系式. 色谱. 1994,12(1):43.
[11] Baeza-Bazeza JJ, Garcia-Alvarez-Coque MC. Prediction of peak shape as a function of retention in reversed-phase liquid chromatography. J. Chromatogr. A. 2004,1022(1-2):17-24.
[12] 张玉奎,董礼孚,包绵生,周桂敏,林从敬,卢佩章. 分析测试通报. 1984,3:16-19.
[13] 邹汉法. 色谱专家系统的理论基础. 中国科学院大连化学物理研究所博士论文. 1989.
[14] Folley JP, Dorsey JG. A review of the exponentially modified Gaussian (EMG) function: evaluation and subsequent calculation of universal data. J. Chromatogr. Sci. 1984,22(1):40-46.
[15] 马小琼. 人参化学物质基础分析方法研究. 中国科学院大连化学物理研究所博士论文. 2005.