水稻SSR分子标记遗传距离与产量杂种优势相关性研究
摘要
本文利用分子标记技术研究遗传距离与杂种优势的关系,以期为杂种优势的预测提供有用的信息。本研究利用筛选出的48个SSR标记,分别对三系杂交稻的汕优系列组合和协优系列组合共14个亲本基因型进行DNA多态性分析,研究其双亲遗传距离与F1杂种产量优势的相关关系,探讨利用亲本间分子遗传距离预测F1杂种优势的可行性。取得的主要结果如下:
1.用48对SSR引物共扩增出136个条带,其中129个具有多态性,平均每对引物能扩增出2.83个条带,其中多态性条带2.69条,多态性比例为94.85%。14个亲本间的SSR标记遗传距离变幅在0.50-0.88之间,平均距离为0.667,其中遗传距离最大的组合是协青早A和7954,他们的遗传距离是0.88;遗传距离最小的组合是协青早A和2DZ057,他们的遗传距离是0.50。
2.通过对15个F1组合农艺性状的分析表明:每株总粒数、实粒数、有效穗数及穗长的变异系数较高;株高与穗长、每株总粒数与每株实粒数呈显著正相关;每株实粒数与理论产量、实际产量呈极显著正相关。。
3.SSR标记亲本遗传距离与产量性状的相关分析表明:在一定遗传距离范围内,杂交水稻的产量与遗传距离呈现正相关。
In order to provide useful information for predicting heterosis, we studied the relations of genetic distance and heterosis using molecular marker technology. We did DNA polymorphism analysis about 14 parental genotypes of three-line hybrid rice combination, Xieyou and Shanyou, using 48 SSR primers. Furtherly we did some research about relevance between parental rice genetic distance and production of the F1 rice, and we explored the possibility of using parental rice genetic distance to predict production of the F1 rice.
The main topics of this thesis are as follows.
1. The PCR result of the 48 SSR-marked primers showed 136 PCR amplified bands (129 bands have polymorphism). In another word the PCR result of each primer has 2.83 bands (2.69 bands have the polymorphism, which take up for 94.85 percents). The range of the genetic distance of the 14 parental SSR markers is from 0.50 to 0.88 (0.667 on average). Of all the the combination, Xie A and 7954 has longest genetic distance, which is 0.88 and Xie A and 2DZ057 has the shortest genetic distance, which is 0.50.
2. The 15 F1 combination analysis result indicated that total grains of each plant, true grains of each plant, number of productive ear and ear length had relatively bigger coefficient of variation. What’s more, plant height and panicle length, total grains of each plant, true grains of each plant showed a significant positive correlation. The true grains of each plant, theoretical production and real production show an extremely significant correlation.
3. The relevant analysis of the genetic distance of the parental SSR marker and the production showed that there was a positive correlation between genetic distance of rice parents and production of the F1 rice in certain extent.
1.用48对SSR引物共扩增出136个条带,其中129个具有多态性,平均每对引物能扩增出2.83个条带,其中多态性条带2.69条,多态性比例为94.85%。14个亲本间的SSR标记遗传距离变幅在0.50-0.88之间,平均距离为0.667,其中遗传距离最大的组合是协青早A和7954,他们的遗传距离是0.88;遗传距离最小的组合是协青早A和2DZ057,他们的遗传距离是0.50。
2.通过对15个F1组合农艺性状的分析表明:每株总粒数、实粒数、有效穗数及穗长的变异系数较高;株高与穗长、每株总粒数与每株实粒数呈显著正相关;每株实粒数与理论产量、实际产量呈极显著正相关。。
3.SSR标记亲本遗传距离与产量性状的相关分析表明:在一定遗传距离范围内,杂交水稻的产量与遗传距离呈现正相关。
In order to provide useful information for predicting heterosis, we studied the relations of genetic distance and heterosis using molecular marker technology. We did DNA polymorphism analysis about 14 parental genotypes of three-line hybrid rice combination, Xieyou and Shanyou, using 48 SSR primers. Furtherly we did some research about relevance between parental rice genetic distance and production of the F1 rice, and we explored the possibility of using parental rice genetic distance to predict production of the F1 rice.
The main topics of this thesis are as follows.
1. The PCR result of the 48 SSR-marked primers showed 136 PCR amplified bands (129 bands have polymorphism). In another word the PCR result of each primer has 2.83 bands (2.69 bands have the polymorphism, which take up for 94.85 percents). The range of the genetic distance of the 14 parental SSR markers is from 0.50 to 0.88 (0.667 on average). Of all the the combination, Xie A and 7954 has longest genetic distance, which is 0.88 and Xie A and 2DZ057 has the shortest genetic distance, which is 0.50.
2. The 15 F1 combination analysis result indicated that total grains of each plant, true grains of each plant, number of productive ear and ear length had relatively bigger coefficient of variation. What’s more, plant height and panicle length, total grains of each plant, true grains of each plant showed a significant positive correlation. The true grains of each plant, theoretical production and real production show an extremely significant correlation.
3. The relevant analysis of the genetic distance of the parental SSR marker and the production showed that there was a positive correlation between genetic distance of rice parents and production of the F1 rice in certain extent.
引文
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