薏苡附子败酱散的药效物质基础、制备工艺及药效学研究
|
摘要
本文对白花败酱草的化学成分进行了较为系统和全面的研究,分离了42个化合物,鉴定了其中的39个;建立了部分化学成分的高速逆流色谱快速分离制备技术平台;在此基础上优选了薏苡附子败酱散活性组分的制备工艺;与此同时,考察了分离所得部分化合物和薏苡附子败酱散活性组分的体外抗氧化、体外体内抗肿瘤活性;并初步制订了该活性组分的质量标准。
The systemic and comprehensive chemical research from from Patrinia villosa Juss was carried out, and 42 compounds were obtained and 39 of them were identified, and the rapid preparation platforms of some compounds have been established by highspeed counter-current chromatography and the preparation protocol of the active fraction of the famous Chinese medicine formular YiyiFuziBaijiangSan has also been established. In the meanwhile, the antioxidant and anticancer activities of the obtained compounds from the plant and the active fraction were all evaluated, and the quality standard of the active fraction was also established.
The systemic and comprehensive chemical research from from Patrinia villosa Juss was carried out, and 42 compounds were obtained and 39 of them were identified, and the rapid preparation platforms of some compounds have been established by highspeed counter-current chromatography and the preparation protocol of the active fraction of the famous Chinese medicine formular YiyiFuziBaijiangSan has also been established. In the meanwhile, the antioxidant and anticancer activities of the obtained compounds from the plant and the active fraction were all evaluated, and the quality standard of the active fraction was also established.
引文
[1] 王安.薏苡附子败酱散治疗痢疾.浙江中医学院学报,1995,19(5):26.
[2] 李承宽.薏苡附子败酱散加味治疗腹腔脓肿58例疗效观察.青海医药杂志,2004,34(1):20.
[3] 陈华.加味薏苡附子败酱散治愈细菌性肝脓肿1例.中国民间疗法,2003,11(12):40.
[4] 赵铎.薏苡附子败酱散治疗克隆氏病.中国乡村医生,2000,15(4):38.
[5] 钱惠泉.薏苡附子败酱散加味治疗溃疡性结肠炎36例.河北中医,2005,37(3):196.
[6] 高一明.薏苡附子败酱散治疗溃疡性结肠炎.中医药研究,2001,17(4):27.
[7] 王颖.薏苡附子败酱散治疗劳淋28例.中国中医药科技,2001,8(3):140.
[8] 张涛,张春梅.薏苡附子败酱散治疗慢性盆腔炎56例.陕西中医,1993,14(12):533.
[9] 牟重临.加味薏苡附子败酱散治疗慢性盆腔炎67例临床观察.中国民间疗法,1997,4,49.
[10] 国家中医药管理局《中华本草》编委会.上海:上海科学技术出版社,1999,329.
[11] 陈宁,李毓,邱健行,等.薏苡仁酯和5-Fu抑制人鼻咽癌细胞增值的增效作用.中医药研究,1999,15(6):45.
[12] 李毓,邱健行,熊带水.薏苡仁酯和顺铂抑制人鼻咽癌细胞增值的协同作用.广东药学院学报,1999,15(4):285.
[13] 王英杰,张闯.薏苡仁酯对荷瘤小鼠红细胞免疫功能的影响.黑龙江医药,1999,12(6):343.
[14] 刘嘉湘,廖美林,严德均,等.康莱特注射液治疗原发性肺癌Ⅱ期临床总结.中药新药与临床药理,1995,6(4):17.
[15] 闫爱荣,宋志萍.附子的药理研究.实用中西医结合杂志,1994,7(11):697.
[16] 何福江,杨建萍,田义杰.败酱属植物化学成分与药理研究概况.甘肃医药,1993,12(3):161.
[17] 姜泓,初正云,王虹霞,等.黄花败酱的化学成分.中草药,2003,34(11):978.
[18] 初正云,姜泓 刘英勃,等.黄花败酱化学成分的分离与鉴定.中药材,2003,26(6):411.
[19] 杨波,丁立新.黄花败酱中酰化新皂苷的分离与鉴定.中草药,2002,33(8):685.
[20] 杨波,陈英杰.黄花败酱中新皂苷的分离与鉴定.中草药,2000,31(1):1.
[21] 雷海民,朱蓉.异叶败酱化学成分研究.中国药学杂志,1997,32(5):271.
[22] 雷海民,朱蓉.异叶败酱的生药学研究.西安医科大学学报,1996,17(3):316.
[23] 雷海民,朱蓉.异叶败酱黄酮类成分的研究.西北药学杂志,1995,10(4):154.
[24] 李廷钊,张卫东,顾正兵,等.糙叶败酱中木脂素成分的研究.中草药,2005,36(3):38.
[25] 顾正兵,杨根金.糙叶败酱碱的分离与结构鉴定.药学学报,2002,37(11):867.
[26] 顾正兵,丛海英.糙叶败酱化学成分的研究.中草药,2002,33(9):781.
[27] 顾正兵,陈新建.糙叶败酱活性成分的研究.中药材,2002,25(3):178.
[28] 徐成俊,曾宪仪,于德泉.白花败酱草的化学成分研究.药学学报,1985,20(9):652.
[29] H. Tagachi, Y. Yokokawa, T. Endo. Studies on the constituents of Patrnina villosa Juss. Yakugaku- Zasshi, 1973, 93 (5): 607.
[30] 田智勇,曹继华,董成明,等.白花败酱草和单药败酱挥发油化学成分分析.时珍国医国药,2005,16(8):738.
[31] 徐洁,周方钦,江汉明,等.白花败酱草总皂苷提取工艺研究.山东中医药大学学报,2005,29(3):227.
[32] 徐洁,周方钦.白花败酱草中总黄酮的提取研究.广州食品工业科技,2004,20(4):42.
[33] 原永芳,李修禄,柳正良.超临界流体萃取法与高效液相色谱法分析延胡索中延胡索乙素的含量.药学学报,1996,31(4):282.
[34] 何春茂,粱忠云.用超临界CO_2萃取技术提取青蒿素的研究.中草药,1999,7(30):497.
[35] 邓启焕,高勇.第二类超临界流体萃取银杏叶有效成分的实验研究.中草药,1999,30(6):419.
[36] 李军,冯耀声.超临界CO_2萃取茶多酚的研究.天然产物研究与开发,1996,8(3):42.
[37] 纪松岗,柴逸峰,吴玉田,等.超临界流体萃取—胶束电动毛细管色谱法分离测定大黄中蒽酣类组分的含量.分析化学,1998,26(11):1388.
[38] 袁海龙,柳正良,张纯,等.超临界流体萃取—HPLC法测定何首乌中大黄酸、大黄素、大黄素甲醚的含量.中草药,1999,30(4):258.
[39] S. M. Pourmortazavi, M. Ghadiri, S. S. Uajimirsadeghi. Supercritical fluid extraction of volatile components from Bunium persicum Boiss (Black cumin) and Mespilus germanica L. (medlar) seeds. J. Food Comp. Anal. , 2005, 18 (5): 439.
[40] M. Khajeh, Y. Yamini, N. Bahramifar, F. Sefidkon, M. R. Pirmoradei. Comparison of essential oils compositions of Ferula assa-foetida obtained by supercritical carbon dioxide extraction and hydrodistillation methods. Food Chem. , 2005, 91 (4): 639.
[41] M. Khajeh, Y. Yamini, F. Sefidkon, N. Bahramifar. Comparison of essential oils compositions of Carum copticum obtained by supercritical carbon dioxide extraction and hydrodistillation methods. Food Chem. , 2004, 86 (4): 587.
[42] N. Aghel, Y. Yamini, A. Hadjiakhoondi, S. M. Pourmortazavi. Supercritical carbon dioxide extraction of Mentha pulegium L. essential oil. Talanta, 2004, 62 (2): 407.
[43] 石忠峰,陈蔚文,李卫民,等.大孔吸附树脂纯化黄芪总皂苷的研究.中草药,2005,36(8):1320.
[44] 荆宇,赵余庆.大孔吸附树脂提取纯化胡芦巴中总皂苷的工艺研究.中药研究与信息,2005,7(6):19.
[45] 马挺军,屠鹏飞,吕飞杰.大孔吸附树脂分离富集土贝母总皂苷的研究.中国中药杂志,2005,30(10):744.
[46] 舒孝顺,高中洪,杨祥良.用大孔吸附树脂分离菝藜总皂苷的条件优化.中国中药杂志,2005,30(1):30.
[47] 葛淑兰,田景振.大孔吸附树脂对淫羊藿总黄酮的分离纯化工艺研究.中国药学杂志,2005,40(5):365.
[48] 李成网,李清华.大孔吸附树脂分离纯化葛根素的研究.医药导报,2005,24(5):432.
[49] 林天慕,管清香,张三奇.大孔吸附树脂分离纯化独一味总黄酮的研究.中国药学杂志,2005,40(4):264.
[50] 纵伟,夏文水,崔宝良.大孔吸附树脂纯化大叶紫薇叶中总三萜的研究.食品工业科技,2005,26(9):49.
[51] 尹蓉莉,江靖,张淑儒,等.大孔吸附树脂纯化白芍总苷的工艺研究.时珍国医国药,2005,16(7):610.
[52] 王瑞芳,欧来良,孔德领,等.大孔吸附树脂对喜树果中喜树碱和喜果苷的分离纯化研究.中草药,2005,36(6):849.
[53] 李建成,贺莲,刘平安.大孔吸附树脂富集纯化博落回总生物碱的工艺研究.湖南中医杂志,2004,20(2):60.
[54] 张天佑.逆流色谱技术,北京,北京科学技术出版社,1991,267.
[55] F. Q. Yang, T. Y. Zhang, R. Zhang, Y. Ito. Application of analytical and preparative high-speed countercurrent chromatography for separation of alkaloids from Coptis chinensis Franch. J. Chromatogr. A, 1998, 829 (1-2): 137.
[56] T. Y. Zhang, D. G. Cai, Y. Ito. Separations of flavonoids and alkaloids in medicinal herbs by high-speed counter-current chromatography, J. Chromatogr. A, 1988, 435, 159.
[57] R. Liu, X. Chu, A. L. Sun, L. Y. Kong. Preparative isolation and purification of alkaloids from the medicinal herb Evodia rutaecarpa (Juss.) Benth by high-speed counter-current chromatography, J. Chromatogr. A, 2005, 1074 (1-2): 139.
[58] F. Q. Yang, Y. Ito. Preparative separation of lappaconitine, ranaconitine, N-deacety-Ilappaconitine and N-deacetylranaconitine from crude alkaloids of sample Aconiturn sinomontanum Naki by high-speed counter-current chromatography. J. Chromatogr. A, 2002, 943 (2): 219.
[59] X. Wang, F. W. Li, H. X. Zhang, et al. Preparative isolation and purification of polymethoxylated flavones from Tangerine peel using high-speed counter-current chromatography, J. Chromatogr. A, 2005, 1090 (1-2): 188.
[60] M. B. Zhao, Y. Ito, P. F. Tu. Isolation of a novel flavanone 6-glucoside from the flowers of carthamus tinctorium (Honghua) by high-speed counter-current chromatography, J. Chromatogr. A 2005, 1091 (1-2): 193.
[61] X. Wang, C. Cheng, Q. L. Sun, et al. Isolation and purification of four flavonid constituents from the flowers of Paeonia suffruticosa by high-speed counter-current chromatography, J. Chromatogr. A, 2005, 1075 (1-2): 127.
[62] X. F. Ma, W. X. Tian, L. H. Wu, X. L. Cao, Y. Ito. Isolation of quercetin-3-O-L-rhamnoside from Acer truncatum Bunge by high-speed counter-current chromatography, J. Chrornatogr. A, 2005, 1070 (1-2): 211.
[63] R. M. Liu, A. F. Li, A. L. Sum, et al. Preparative isolation and purification of three flavonoids from the Chinese medicinal plant Epimedium koreamum Nakai by high-speed counter-current chromatography. J. Chromatogr. A, 2005, 1064 (1): 53.
[64] J. Y. Peng, G. R. Fan, Y. T. Wu. Isolation and purification of clemastanin B and indigoticoside A from Radix Isatidis by high-speed counter-current chromatography. J. Chromatogr. A, 2005, 1091 (1-2): 89.
[65] J. Y. Peng, G. R. Fan, L. P. Qu, et al. Application of preparative high-speed counter-current chromatography for isolation and separation of schizandrin and gomisin A from Schisandra chinensis. J. Chromatogr. A, 2005, 1082 (2): 203.
[66] T. H. Huang, P. N. Shen, Y. J. Shen. Preparative separation and purification of deoxyschisandrin and γ-schisandrin from Schisandra chinensis (Turcz) Baill by high-speed counter-current chromatogramphy. J. Chromatogr. A, 2005, 1066 (1-2): 239.
[67] R. M. Liu, L. Feng, A. L. Sun, et al. Preparative isolation and purification of coumarins from Peucedanum praeruptorum Bunn by high-speed counter-current chromatography. J. Chromatogr. A, 2004, 1057 (1-2): 89.
[68] R. M. Liu, L. Feng, A. L. Sun, et al. Preparative isolation and purification of coumarins from Conidium monnieri (L.) Cusson by high-speed counter-current chromatography. J. Chromatogr. A, 2004, 1055 (1-2): 71.
[69] 胡幼华,李瑜.灰苞蒿化学成分的研究.中国中药杂志,1994,19(3):164.
[70] H. Kizh, H. Shimana, T. Tomimori. Studies on the constituents of Clematis species Ⅵ. The constituents of Clematics stans Sieb. et. : Zucc. Chem. Pharm. Bull. , 1995, 43 (12): 2187.
[71] 王祝举,赵玉荧,涂光忠,等.夏枯草化学成分的研究.药学学报,1999,34(9):679.
[72] H. Kojima, H. Ogura, Configuratianal studies on hydroxy groups at C-2, 3 and 23 or 24 of oleanene and ursine-type triterpenes by NMR spectroscopy. Phytochemistry, 1989, 28 (6): 1703.
[73] 鞠建华,周亮,林耕,等.枇杷叶中三萜酸类成分及其抗炎、镇咳活性研究.中国药学杂志,2003,38(10):752.
[74] D. Takeshi, I. Takako, K. Shizuka, et al. The constituents of Eucomunia ulmoides OLIV. Ⅴ. isolation of dohydroxydehydrodiconiferyl alcohol isomers and phenolic compounds. Chem. Pharm. Bull. , 1987, 35, 1785.
[75] 张治针,果德安,李长龄,等.滇白珠化学成分研究.中草药,1999,30(3):167.
[76] 沙东旭,刘英华,王龙顺,等.叶下珠化学成分研究.沈阳药科大学学报,2000,17(3):176.
[77] 裵月湖,韩冰,冯宝化,等.狼毒大戢化学成分的研究.中草药,2002,33(7):591.
[78] 蓝树彬.齿叶泥花草化学成分的研究.中国中药杂志,1996,2l(1):38.
[79] 催益冷,穆青,胡昌奇,等.红花锦鸡儿化学成分的研究.中国药学杂志,2004,39(3):173.
[80] 田景奎,吴丽敏,王爱武,等.猫爪草化学成分的研究.中国药学杂志,2004,39(3):661.
[81] 何春年,王春兴,郭顺星,等.福建金线莲的化学成分研究Ⅱ.中国中药杂志,2005,30(10):761.
[82] 王力生,马学敏,郭亚健,等.苦玄参的化学成分研究.中国中药杂志,2004,29(2):149.
[83] 黄丽瑛,吕植桢,李继彪,等.中药金银花化学成分的研究.中草药,1996,27(11):645.
[84] Z. Abdedatif, J. Akino, B. Bernard. DNA Toposiomerase I inhibitors: cytottoxic flavones from Ccethedon Fannaensis. J. Nat. Prod. , 1996, 59, 701.
[85] 任玉琳,杨俊山.西藏雪莲花化学成份研究Ⅱ.中国药学杂志,2001,36(9):590.
[86] 高玉敏,穆惠军,王名洲,等.金银仡化学成分的研究.中草药,1995,26(11):568.
[87] K. Toshihiro, M. Tomomi, M. Sligeru, et al. An effective synthesis of iossrienthin: the negioselective synthesis of a 6-C-glucosylflavone. Carbohy. Res. , 2000, 329, 507.
[88] 林励,谢宁,程紫骅.木豆黄酮类成分的研究.中国药科大学学报,1999,30(1):21.
[89] M. R. Parthasathy, T. R. Seshadri, New isoflavonoid glucosides from Dalbergia paniculata. Phytochemistry, 1976, 15, 1025.
[90] 史文清,左春阳.黄连木叶化学成分研究.中国中药杂志,1992,17(7):442.
[91] B. Gomotsang, C. W. W. Cornelius, R. T. M. Runner. Flavonoids from the stem bark of Bolusanthus speciosus. Pyhtochemistry, 2001, 56, 837.
[92] S. Yoshiaki, M. Satoko, K. Manki, et al. Four isoflavanones from roots of Sophora tetraptera. Phytochemistry, 1999, 50, 695.
[93] V. K. Saxena, M. Singhal. Novel prenylated flavonoid from stem of Pithecellobium dulce. Fitoterapia, 1999, 20 (1): 98.
[94] G. W. Peter, E. N. Mahmand. Unusual flavonoids from Lonchocarpus orotinus Seeds. Phytochemistry, 1987, 26 (4): 1189.
[95] W. Li, A. Yoshihisa, Y. Takafumi. Flavonoid constituents from Glycyrrhiza glabra hairy root cuitures. Phytochemistry, 2000, 55, 447.
[96] B. Gomotsang, C. W. W. Cornelius, R. T. M. Runner. Flavonoids from the stem bark of Bolusanthus speciosus. Pyhtochemistry, 2001, 56, 837.
[97] D. O. Didem, A. Mustafa, A. GOknur, et al. Evaluation of hepatoprtective effect of Gentiana olivieri herbs on subacute administration and isolation of active principle. Life Sci., 2003, 72 (20): 2273.
[98] M. S. C. Pedras, P. B. Chumala, M. Cuchy, Phytoalexins from Thlaspi arvense, a wild crucifer vesistant to viruleut Leptosphaeria maculans: structures, synthese and antifunael activity. Phytochemistry, 2003, 64 (5): 949.
[99] 田智勇,曹继华.黄花败酱和异叶败酱挥发油的研究.河南大学学报:医学科学版,2004,23(1):35.
[100] 张文蔚,陈虎彪.岩败酱挥发性化学成分的研究.中药材,1999,22(8):403.
[101] 张宏利,张振杰.缬草挥发油化学成分的研究.天然产物研究与开发,1993,5(3):21.
[102] 何福江,李洪刚.糙叶败酱化学成分研究Ⅰ:糙叶败酱挥发油的化学成分.药物分析杂志,1991,11(2):67.
[103] 陈燕萍,曾靖,叶和扬.白花败酱草水提液中枢抑制作用的研究.中国药物与临床,2005,5(6):439.
[104] 钟星明,蒋绍祖,黄玉珊,等.白花败酱草提取物对小鼠睡眠功能和白发活动的影响.中国临床康复,2004,8(30):6688.
[105] 李大鹏,吴伯千.超临界CO_2萃取薏苡仁油工艺条件研究.中国现代应用药学杂志,2005,22(1):17.
[106] 王毅红,王绪英.赤胫散中总黄酮的含量测定.唐山师范学院学报,2005,27(5):44.
[107] 鄢英慧,刘紫英,沈小苹.紫外分光光度法测定保肾口服液巾总黄酮含景.福建中医药,2005,36(4):43.
[108] 陈志斌,黄艳萍,袁萍.冠心宁片中总黄酮的含量测定.时珍国医国药,2005,16(4):322.
[109] 黄衍民,张柞建,郭爱民,等.脑灵胶囊中银杏总黄酮成分的含量测定.中国医院药学杂志,2004,24(10):620.
[110] 刘冰,马合木提·买.骆驼蓬总生物碱颗粒剂的制备及含量测定.新疆医科大学学报,2003,26(1):81.
[111] 于超,张明.紫外分光光度法测定不同产地半夏中总生物碱的含量.时珍国医国药,2002,13(2):73.
[112] 张荣,汤秋华.紫外分光光度法测定雷公藤口服液中总生物碱的含量.华西药学杂志,2000,15(2):133.
[113] 赵丹.中药复方二仙汤中总黄酮和总皂苷的含量测定.东南国防医药,2005,7(3):189.
[114] 丁青龙,李莹.紫外分光光度法测定复方银杏胶囊中绞股蓝总皂苷的含量.中国医学研究与临床,2004,2(14):5.
[115] 孔燕君,洪美凤.紫外分光光度法测定人参及三七中总皂苷的含量.中国现代应用药学,2000,17(1):51.
[116] 杨雄志.水三仙口服液中总多糖的含量测定.实用中西医结合临床,2005,5(4):77.
[117] 张功武,陈洁.糖肾康颗粒中总多糖的含量测定.现代中药研究与实践,2004,18(5):60.
[118] 王宇环,曾白林.扶正抑瘤颗粒中总多糖的含量测定研究.中草药,2001,32(12):1076.
[119] B. Hallivwell, Antioxidants in human health and disease. Annu. Rev. Nutr. 1996, 16, 33.
[120] Poeggeler B, R. J. Reiter, D. X. Tan, et al. Melatonin, hydroxyl radical-mediated oxidative damage, and aging: a hypothesis. J. Pineal. Res., 1993, 14, 151.
[121] R. L. Zgeng, S. A. Lesko. DNA damage included in mammalian cells by active oxygen species. Sci. Sin. Chin. Ed. , 1988, 31, 676.
[122] Y. C. Zhou, R. L. Zheng. Phenolic compounds and an analog as superoxide anion scavenger and antioxidants. Biochem. Pharmacol. , 1991, 42, 1177.
[123] L. W. Gu, X. C. Wang. Antioxidant activity and components of Sadvia plebeuar Br.-a Chinese herb. Foog Chem. , 2001, 73, 299.
[124] G. R. Schinella, H. A. Tournier, J. M. Prieto, et al. Antioxidant activity of anti-inflammatory plant extracts. Life Sci. , 2002, 70, 1023.
[125] K. G. Dufall, B. T. Ngadjui, K. F. Simeon, et al. Antioxidant activity of prenylated flavonoids from the West African medicinal plant Dorstenia mannii, J. Ethnopharmacol. , 2003, 87, 67.
[126] B. Alessandra, F. Gelsomina, M. lvano, et al. Antioxidant and free scavenging activity of flavonol glycosides from different Aconitum species, J. Ethnopharmacol. , 2003, 86, 63.
[127] 秦德安,苏丹,王晓玲.橙皮苷对羟基自由基的清除作用.中国药学杂志,1996,31(7):397.
[128] 田京伟,杨建雄.白藜芦醇苷的体外抗氧化活性.中草药,2001,32(10):918.
[129] Y. J. Wu, W. G. Li, Z. M. Zhang, et al. Antioxidantive activity of 4-oxy and 4-hydroxy-nitroxides in tissues and erthrocytes from rats. Acta Pharmacol. Sin., 1997, 18, 150.
[130] J. Li, R. L. Zheng, Z. M. Liu, et al. Scavenging effects of phenypropanoid glycosides on superoxide and its antioxidant effect. Acta Pharmacol. Sin., 1992, 13, 427.
[131] 张晓平,蒲小平,擢所迪,朱玉珍.抑瘤延生散抗S180肿瘤作用的初步研究.中国中药杂志,2002,27(11):852.
[132] 李选社,薛晚利,张健,等.益气散结解毒汤对S180肉瘤抑制作用的实验研究.西安交通大学学报,2003,24(6):624.
[133] 张学武,张玉梅,关丽萍,等.珍珠梅活性成分的提取分离及体内抑瘤作用的研究.中药材,2004,27(1):37.
[134] 许正新,许俊,刘晓梅,等.中药复方Ⅰ号对荷瘤鼠肿瘤生长抑制作用的试验研究.实用临床医药杂志,2004,8(1):48.
[135] G. F. Zhou, H. Xin, W. X. Sheng, Y. P. Sun, Z. E. Li, Z. H. Xu. In vivo growth-inhibition of S180 tumor by mixture of 5-Fu and low molecular λ-carrageenan from Chondrus ocellatus. Pharmocol. Res., 2005, 51 (2): 153.
[136] S. J. Liu, X. Luo, D. X. Li, J. Zhang, D. L. Qiu, et al. Tumor inhibition and improved immunity in mice tereated with flavone from Cirsium japonicum DC. Int. Immunopharm., in press.
[137] 李志孝,黄成锅,蔡育军,等.天门冬多糖的化学结构及体外抗氧化活性.药学学报,2000,35(5):358.
[138] 塔娜,李蜀眉,田维中,等.桔皮中黄酮类化合物抗氧化活性的研究.内蒙古农业大学学报,2003,24(2):96.
[2] 李承宽.薏苡附子败酱散加味治疗腹腔脓肿58例疗效观察.青海医药杂志,2004,34(1):20.
[3] 陈华.加味薏苡附子败酱散治愈细菌性肝脓肿1例.中国民间疗法,2003,11(12):40.
[4] 赵铎.薏苡附子败酱散治疗克隆氏病.中国乡村医生,2000,15(4):38.
[5] 钱惠泉.薏苡附子败酱散加味治疗溃疡性结肠炎36例.河北中医,2005,37(3):196.
[6] 高一明.薏苡附子败酱散治疗溃疡性结肠炎.中医药研究,2001,17(4):27.
[7] 王颖.薏苡附子败酱散治疗劳淋28例.中国中医药科技,2001,8(3):140.
[8] 张涛,张春梅.薏苡附子败酱散治疗慢性盆腔炎56例.陕西中医,1993,14(12):533.
[9] 牟重临.加味薏苡附子败酱散治疗慢性盆腔炎67例临床观察.中国民间疗法,1997,4,49.
[10] 国家中医药管理局《中华本草》编委会.上海:上海科学技术出版社,1999,329.
[11] 陈宁,李毓,邱健行,等.薏苡仁酯和5-Fu抑制人鼻咽癌细胞增值的增效作用.中医药研究,1999,15(6):45.
[12] 李毓,邱健行,熊带水.薏苡仁酯和顺铂抑制人鼻咽癌细胞增值的协同作用.广东药学院学报,1999,15(4):285.
[13] 王英杰,张闯.薏苡仁酯对荷瘤小鼠红细胞免疫功能的影响.黑龙江医药,1999,12(6):343.
[14] 刘嘉湘,廖美林,严德均,等.康莱特注射液治疗原发性肺癌Ⅱ期临床总结.中药新药与临床药理,1995,6(4):17.
[15] 闫爱荣,宋志萍.附子的药理研究.实用中西医结合杂志,1994,7(11):697.
[16] 何福江,杨建萍,田义杰.败酱属植物化学成分与药理研究概况.甘肃医药,1993,12(3):161.
[17] 姜泓,初正云,王虹霞,等.黄花败酱的化学成分.中草药,2003,34(11):978.
[18] 初正云,姜泓 刘英勃,等.黄花败酱化学成分的分离与鉴定.中药材,2003,26(6):411.
[19] 杨波,丁立新.黄花败酱中酰化新皂苷的分离与鉴定.中草药,2002,33(8):685.
[20] 杨波,陈英杰.黄花败酱中新皂苷的分离与鉴定.中草药,2000,31(1):1.
[21] 雷海民,朱蓉.异叶败酱化学成分研究.中国药学杂志,1997,32(5):271.
[22] 雷海民,朱蓉.异叶败酱的生药学研究.西安医科大学学报,1996,17(3):316.
[23] 雷海民,朱蓉.异叶败酱黄酮类成分的研究.西北药学杂志,1995,10(4):154.
[24] 李廷钊,张卫东,顾正兵,等.糙叶败酱中木脂素成分的研究.中草药,2005,36(3):38.
[25] 顾正兵,杨根金.糙叶败酱碱的分离与结构鉴定.药学学报,2002,37(11):867.
[26] 顾正兵,丛海英.糙叶败酱化学成分的研究.中草药,2002,33(9):781.
[27] 顾正兵,陈新建.糙叶败酱活性成分的研究.中药材,2002,25(3):178.
[28] 徐成俊,曾宪仪,于德泉.白花败酱草的化学成分研究.药学学报,1985,20(9):652.
[29] H. Tagachi, Y. Yokokawa, T. Endo. Studies on the constituents of Patrnina villosa Juss. Yakugaku- Zasshi, 1973, 93 (5): 607.
[30] 田智勇,曹继华,董成明,等.白花败酱草和单药败酱挥发油化学成分分析.时珍国医国药,2005,16(8):738.
[31] 徐洁,周方钦,江汉明,等.白花败酱草总皂苷提取工艺研究.山东中医药大学学报,2005,29(3):227.
[32] 徐洁,周方钦.白花败酱草中总黄酮的提取研究.广州食品工业科技,2004,20(4):42.
[33] 原永芳,李修禄,柳正良.超临界流体萃取法与高效液相色谱法分析延胡索中延胡索乙素的含量.药学学报,1996,31(4):282.
[34] 何春茂,粱忠云.用超临界CO_2萃取技术提取青蒿素的研究.中草药,1999,7(30):497.
[35] 邓启焕,高勇.第二类超临界流体萃取银杏叶有效成分的实验研究.中草药,1999,30(6):419.
[36] 李军,冯耀声.超临界CO_2萃取茶多酚的研究.天然产物研究与开发,1996,8(3):42.
[37] 纪松岗,柴逸峰,吴玉田,等.超临界流体萃取—胶束电动毛细管色谱法分离测定大黄中蒽酣类组分的含量.分析化学,1998,26(11):1388.
[38] 袁海龙,柳正良,张纯,等.超临界流体萃取—HPLC法测定何首乌中大黄酸、大黄素、大黄素甲醚的含量.中草药,1999,30(4):258.
[39] S. M. Pourmortazavi, M. Ghadiri, S. S. Uajimirsadeghi. Supercritical fluid extraction of volatile components from Bunium persicum Boiss (Black cumin) and Mespilus germanica L. (medlar) seeds. J. Food Comp. Anal. , 2005, 18 (5): 439.
[40] M. Khajeh, Y. Yamini, N. Bahramifar, F. Sefidkon, M. R. Pirmoradei. Comparison of essential oils compositions of Ferula assa-foetida obtained by supercritical carbon dioxide extraction and hydrodistillation methods. Food Chem. , 2005, 91 (4): 639.
[41] M. Khajeh, Y. Yamini, F. Sefidkon, N. Bahramifar. Comparison of essential oils compositions of Carum copticum obtained by supercritical carbon dioxide extraction and hydrodistillation methods. Food Chem. , 2004, 86 (4): 587.
[42] N. Aghel, Y. Yamini, A. Hadjiakhoondi, S. M. Pourmortazavi. Supercritical carbon dioxide extraction of Mentha pulegium L. essential oil. Talanta, 2004, 62 (2): 407.
[43] 石忠峰,陈蔚文,李卫民,等.大孔吸附树脂纯化黄芪总皂苷的研究.中草药,2005,36(8):1320.
[44] 荆宇,赵余庆.大孔吸附树脂提取纯化胡芦巴中总皂苷的工艺研究.中药研究与信息,2005,7(6):19.
[45] 马挺军,屠鹏飞,吕飞杰.大孔吸附树脂分离富集土贝母总皂苷的研究.中国中药杂志,2005,30(10):744.
[46] 舒孝顺,高中洪,杨祥良.用大孔吸附树脂分离菝藜总皂苷的条件优化.中国中药杂志,2005,30(1):30.
[47] 葛淑兰,田景振.大孔吸附树脂对淫羊藿总黄酮的分离纯化工艺研究.中国药学杂志,2005,40(5):365.
[48] 李成网,李清华.大孔吸附树脂分离纯化葛根素的研究.医药导报,2005,24(5):432.
[49] 林天慕,管清香,张三奇.大孔吸附树脂分离纯化独一味总黄酮的研究.中国药学杂志,2005,40(4):264.
[50] 纵伟,夏文水,崔宝良.大孔吸附树脂纯化大叶紫薇叶中总三萜的研究.食品工业科技,2005,26(9):49.
[51] 尹蓉莉,江靖,张淑儒,等.大孔吸附树脂纯化白芍总苷的工艺研究.时珍国医国药,2005,16(7):610.
[52] 王瑞芳,欧来良,孔德领,等.大孔吸附树脂对喜树果中喜树碱和喜果苷的分离纯化研究.中草药,2005,36(6):849.
[53] 李建成,贺莲,刘平安.大孔吸附树脂富集纯化博落回总生物碱的工艺研究.湖南中医杂志,2004,20(2):60.
[54] 张天佑.逆流色谱技术,北京,北京科学技术出版社,1991,267.
[55] F. Q. Yang, T. Y. Zhang, R. Zhang, Y. Ito. Application of analytical and preparative high-speed countercurrent chromatography for separation of alkaloids from Coptis chinensis Franch. J. Chromatogr. A, 1998, 829 (1-2): 137.
[56] T. Y. Zhang, D. G. Cai, Y. Ito. Separations of flavonoids and alkaloids in medicinal herbs by high-speed counter-current chromatography, J. Chromatogr. A, 1988, 435, 159.
[57] R. Liu, X. Chu, A. L. Sun, L. Y. Kong. Preparative isolation and purification of alkaloids from the medicinal herb Evodia rutaecarpa (Juss.) Benth by high-speed counter-current chromatography, J. Chromatogr. A, 2005, 1074 (1-2): 139.
[58] F. Q. Yang, Y. Ito. Preparative separation of lappaconitine, ranaconitine, N-deacety-Ilappaconitine and N-deacetylranaconitine from crude alkaloids of sample Aconiturn sinomontanum Naki by high-speed counter-current chromatography. J. Chromatogr. A, 2002, 943 (2): 219.
[59] X. Wang, F. W. Li, H. X. Zhang, et al. Preparative isolation and purification of polymethoxylated flavones from Tangerine peel using high-speed counter-current chromatography, J. Chromatogr. A, 2005, 1090 (1-2): 188.
[60] M. B. Zhao, Y. Ito, P. F. Tu. Isolation of a novel flavanone 6-glucoside from the flowers of carthamus tinctorium (Honghua) by high-speed counter-current chromatography, J. Chromatogr. A 2005, 1091 (1-2): 193.
[61] X. Wang, C. Cheng, Q. L. Sun, et al. Isolation and purification of four flavonid constituents from the flowers of Paeonia suffruticosa by high-speed counter-current chromatography, J. Chromatogr. A, 2005, 1075 (1-2): 127.
[62] X. F. Ma, W. X. Tian, L. H. Wu, X. L. Cao, Y. Ito. Isolation of quercetin-3-O-L-rhamnoside from Acer truncatum Bunge by high-speed counter-current chromatography, J. Chrornatogr. A, 2005, 1070 (1-2): 211.
[63] R. M. Liu, A. F. Li, A. L. Sum, et al. Preparative isolation and purification of three flavonoids from the Chinese medicinal plant Epimedium koreamum Nakai by high-speed counter-current chromatography. J. Chromatogr. A, 2005, 1064 (1): 53.
[64] J. Y. Peng, G. R. Fan, Y. T. Wu. Isolation and purification of clemastanin B and indigoticoside A from Radix Isatidis by high-speed counter-current chromatography. J. Chromatogr. A, 2005, 1091 (1-2): 89.
[65] J. Y. Peng, G. R. Fan, L. P. Qu, et al. Application of preparative high-speed counter-current chromatography for isolation and separation of schizandrin and gomisin A from Schisandra chinensis. J. Chromatogr. A, 2005, 1082 (2): 203.
[66] T. H. Huang, P. N. Shen, Y. J. Shen. Preparative separation and purification of deoxyschisandrin and γ-schisandrin from Schisandra chinensis (Turcz) Baill by high-speed counter-current chromatogramphy. J. Chromatogr. A, 2005, 1066 (1-2): 239.
[67] R. M. Liu, L. Feng, A. L. Sun, et al. Preparative isolation and purification of coumarins from Peucedanum praeruptorum Bunn by high-speed counter-current chromatography. J. Chromatogr. A, 2004, 1057 (1-2): 89.
[68] R. M. Liu, L. Feng, A. L. Sun, et al. Preparative isolation and purification of coumarins from Conidium monnieri (L.) Cusson by high-speed counter-current chromatography. J. Chromatogr. A, 2004, 1055 (1-2): 71.
[69] 胡幼华,李瑜.灰苞蒿化学成分的研究.中国中药杂志,1994,19(3):164.
[70] H. Kizh, H. Shimana, T. Tomimori. Studies on the constituents of Clematis species Ⅵ. The constituents of Clematics stans Sieb. et. : Zucc. Chem. Pharm. Bull. , 1995, 43 (12): 2187.
[71] 王祝举,赵玉荧,涂光忠,等.夏枯草化学成分的研究.药学学报,1999,34(9):679.
[72] H. Kojima, H. Ogura, Configuratianal studies on hydroxy groups at C-2, 3 and 23 or 24 of oleanene and ursine-type triterpenes by NMR spectroscopy. Phytochemistry, 1989, 28 (6): 1703.
[73] 鞠建华,周亮,林耕,等.枇杷叶中三萜酸类成分及其抗炎、镇咳活性研究.中国药学杂志,2003,38(10):752.
[74] D. Takeshi, I. Takako, K. Shizuka, et al. The constituents of Eucomunia ulmoides OLIV. Ⅴ. isolation of dohydroxydehydrodiconiferyl alcohol isomers and phenolic compounds. Chem. Pharm. Bull. , 1987, 35, 1785.
[75] 张治针,果德安,李长龄,等.滇白珠化学成分研究.中草药,1999,30(3):167.
[76] 沙东旭,刘英华,王龙顺,等.叶下珠化学成分研究.沈阳药科大学学报,2000,17(3):176.
[77] 裵月湖,韩冰,冯宝化,等.狼毒大戢化学成分的研究.中草药,2002,33(7):591.
[78] 蓝树彬.齿叶泥花草化学成分的研究.中国中药杂志,1996,2l(1):38.
[79] 催益冷,穆青,胡昌奇,等.红花锦鸡儿化学成分的研究.中国药学杂志,2004,39(3):173.
[80] 田景奎,吴丽敏,王爱武,等.猫爪草化学成分的研究.中国药学杂志,2004,39(3):661.
[81] 何春年,王春兴,郭顺星,等.福建金线莲的化学成分研究Ⅱ.中国中药杂志,2005,30(10):761.
[82] 王力生,马学敏,郭亚健,等.苦玄参的化学成分研究.中国中药杂志,2004,29(2):149.
[83] 黄丽瑛,吕植桢,李继彪,等.中药金银花化学成分的研究.中草药,1996,27(11):645.
[84] Z. Abdedatif, J. Akino, B. Bernard. DNA Toposiomerase I inhibitors: cytottoxic flavones from Ccethedon Fannaensis. J. Nat. Prod. , 1996, 59, 701.
[85] 任玉琳,杨俊山.西藏雪莲花化学成份研究Ⅱ.中国药学杂志,2001,36(9):590.
[86] 高玉敏,穆惠军,王名洲,等.金银仡化学成分的研究.中草药,1995,26(11):568.
[87] K. Toshihiro, M. Tomomi, M. Sligeru, et al. An effective synthesis of iossrienthin: the negioselective synthesis of a 6-C-glucosylflavone. Carbohy. Res. , 2000, 329, 507.
[88] 林励,谢宁,程紫骅.木豆黄酮类成分的研究.中国药科大学学报,1999,30(1):21.
[89] M. R. Parthasathy, T. R. Seshadri, New isoflavonoid glucosides from Dalbergia paniculata. Phytochemistry, 1976, 15, 1025.
[90] 史文清,左春阳.黄连木叶化学成分研究.中国中药杂志,1992,17(7):442.
[91] B. Gomotsang, C. W. W. Cornelius, R. T. M. Runner. Flavonoids from the stem bark of Bolusanthus speciosus. Pyhtochemistry, 2001, 56, 837.
[92] S. Yoshiaki, M. Satoko, K. Manki, et al. Four isoflavanones from roots of Sophora tetraptera. Phytochemistry, 1999, 50, 695.
[93] V. K. Saxena, M. Singhal. Novel prenylated flavonoid from stem of Pithecellobium dulce. Fitoterapia, 1999, 20 (1): 98.
[94] G. W. Peter, E. N. Mahmand. Unusual flavonoids from Lonchocarpus orotinus Seeds. Phytochemistry, 1987, 26 (4): 1189.
[95] W. Li, A. Yoshihisa, Y. Takafumi. Flavonoid constituents from Glycyrrhiza glabra hairy root cuitures. Phytochemistry, 2000, 55, 447.
[96] B. Gomotsang, C. W. W. Cornelius, R. T. M. Runner. Flavonoids from the stem bark of Bolusanthus speciosus. Pyhtochemistry, 2001, 56, 837.
[97] D. O. Didem, A. Mustafa, A. GOknur, et al. Evaluation of hepatoprtective effect of Gentiana olivieri herbs on subacute administration and isolation of active principle. Life Sci., 2003, 72 (20): 2273.
[98] M. S. C. Pedras, P. B. Chumala, M. Cuchy, Phytoalexins from Thlaspi arvense, a wild crucifer vesistant to viruleut Leptosphaeria maculans: structures, synthese and antifunael activity. Phytochemistry, 2003, 64 (5): 949.
[99] 田智勇,曹继华.黄花败酱和异叶败酱挥发油的研究.河南大学学报:医学科学版,2004,23(1):35.
[100] 张文蔚,陈虎彪.岩败酱挥发性化学成分的研究.中药材,1999,22(8):403.
[101] 张宏利,张振杰.缬草挥发油化学成分的研究.天然产物研究与开发,1993,5(3):21.
[102] 何福江,李洪刚.糙叶败酱化学成分研究Ⅰ:糙叶败酱挥发油的化学成分.药物分析杂志,1991,11(2):67.
[103] 陈燕萍,曾靖,叶和扬.白花败酱草水提液中枢抑制作用的研究.中国药物与临床,2005,5(6):439.
[104] 钟星明,蒋绍祖,黄玉珊,等.白花败酱草提取物对小鼠睡眠功能和白发活动的影响.中国临床康复,2004,8(30):6688.
[105] 李大鹏,吴伯千.超临界CO_2萃取薏苡仁油工艺条件研究.中国现代应用药学杂志,2005,22(1):17.
[106] 王毅红,王绪英.赤胫散中总黄酮的含量测定.唐山师范学院学报,2005,27(5):44.
[107] 鄢英慧,刘紫英,沈小苹.紫外分光光度法测定保肾口服液巾总黄酮含景.福建中医药,2005,36(4):43.
[108] 陈志斌,黄艳萍,袁萍.冠心宁片中总黄酮的含量测定.时珍国医国药,2005,16(4):322.
[109] 黄衍民,张柞建,郭爱民,等.脑灵胶囊中银杏总黄酮成分的含量测定.中国医院药学杂志,2004,24(10):620.
[110] 刘冰,马合木提·买.骆驼蓬总生物碱颗粒剂的制备及含量测定.新疆医科大学学报,2003,26(1):81.
[111] 于超,张明.紫外分光光度法测定不同产地半夏中总生物碱的含量.时珍国医国药,2002,13(2):73.
[112] 张荣,汤秋华.紫外分光光度法测定雷公藤口服液中总生物碱的含量.华西药学杂志,2000,15(2):133.
[113] 赵丹.中药复方二仙汤中总黄酮和总皂苷的含量测定.东南国防医药,2005,7(3):189.
[114] 丁青龙,李莹.紫外分光光度法测定复方银杏胶囊中绞股蓝总皂苷的含量.中国医学研究与临床,2004,2(14):5.
[115] 孔燕君,洪美凤.紫外分光光度法测定人参及三七中总皂苷的含量.中国现代应用药学,2000,17(1):51.
[116] 杨雄志.水三仙口服液中总多糖的含量测定.实用中西医结合临床,2005,5(4):77.
[117] 张功武,陈洁.糖肾康颗粒中总多糖的含量测定.现代中药研究与实践,2004,18(5):60.
[118] 王宇环,曾白林.扶正抑瘤颗粒中总多糖的含量测定研究.中草药,2001,32(12):1076.
[119] B. Hallivwell, Antioxidants in human health and disease. Annu. Rev. Nutr. 1996, 16, 33.
[120] Poeggeler B, R. J. Reiter, D. X. Tan, et al. Melatonin, hydroxyl radical-mediated oxidative damage, and aging: a hypothesis. J. Pineal. Res., 1993, 14, 151.
[121] R. L. Zgeng, S. A. Lesko. DNA damage included in mammalian cells by active oxygen species. Sci. Sin. Chin. Ed. , 1988, 31, 676.
[122] Y. C. Zhou, R. L. Zheng. Phenolic compounds and an analog as superoxide anion scavenger and antioxidants. Biochem. Pharmacol. , 1991, 42, 1177.
[123] L. W. Gu, X. C. Wang. Antioxidant activity and components of Sadvia plebeuar Br.-a Chinese herb. Foog Chem. , 2001, 73, 299.
[124] G. R. Schinella, H. A. Tournier, J. M. Prieto, et al. Antioxidant activity of anti-inflammatory plant extracts. Life Sci. , 2002, 70, 1023.
[125] K. G. Dufall, B. T. Ngadjui, K. F. Simeon, et al. Antioxidant activity of prenylated flavonoids from the West African medicinal plant Dorstenia mannii, J. Ethnopharmacol. , 2003, 87, 67.
[126] B. Alessandra, F. Gelsomina, M. lvano, et al. Antioxidant and free scavenging activity of flavonol glycosides from different Aconitum species, J. Ethnopharmacol. , 2003, 86, 63.
[127] 秦德安,苏丹,王晓玲.橙皮苷对羟基自由基的清除作用.中国药学杂志,1996,31(7):397.
[128] 田京伟,杨建雄.白藜芦醇苷的体外抗氧化活性.中草药,2001,32(10):918.
[129] Y. J. Wu, W. G. Li, Z. M. Zhang, et al. Antioxidantive activity of 4-oxy and 4-hydroxy-nitroxides in tissues and erthrocytes from rats. Acta Pharmacol. Sin., 1997, 18, 150.
[130] J. Li, R. L. Zheng, Z. M. Liu, et al. Scavenging effects of phenypropanoid glycosides on superoxide and its antioxidant effect. Acta Pharmacol. Sin., 1992, 13, 427.
[131] 张晓平,蒲小平,擢所迪,朱玉珍.抑瘤延生散抗S180肿瘤作用的初步研究.中国中药杂志,2002,27(11):852.
[132] 李选社,薛晚利,张健,等.益气散结解毒汤对S180肉瘤抑制作用的实验研究.西安交通大学学报,2003,24(6):624.
[133] 张学武,张玉梅,关丽萍,等.珍珠梅活性成分的提取分离及体内抑瘤作用的研究.中药材,2004,27(1):37.
[134] 许正新,许俊,刘晓梅,等.中药复方Ⅰ号对荷瘤鼠肿瘤生长抑制作用的试验研究.实用临床医药杂志,2004,8(1):48.
[135] G. F. Zhou, H. Xin, W. X. Sheng, Y. P. Sun, Z. E. Li, Z. H. Xu. In vivo growth-inhibition of S180 tumor by mixture of 5-Fu and low molecular λ-carrageenan from Chondrus ocellatus. Pharmocol. Res., 2005, 51 (2): 153.
[136] S. J. Liu, X. Luo, D. X. Li, J. Zhang, D. L. Qiu, et al. Tumor inhibition and improved immunity in mice tereated with flavone from Cirsium japonicum DC. Int. Immunopharm., in press.
[137] 李志孝,黄成锅,蔡育军,等.天门冬多糖的化学结构及体外抗氧化活性.药学学报,2000,35(5):358.
[138] 塔娜,李蜀眉,田维中,等.桔皮中黄酮类化合物抗氧化活性的研究.内蒙古农业大学学报,2003,24(2):96.