坐骨神经损伤后Nogo-A在相应脊髓节段的表达
摘要
目的：通过切断及切断后再修复坐骨神经，观察在其相应脊髓节段中Nogo-A表达的变化规律，了解在周围神经损伤及修复过程中相应中枢部位Nogo-A的作用规律，为合理的选择周围神经损伤的修复方法和修复时间，有效避免Nogo-A对周围神经损伤修复的影响提供基础理论依据。
方法：选用健康Sprague Dawley(SD)大鼠150只，体重约200克，雌雄不拘，按照实验目的随机分为6组，每组25只。第一组：切断坐骨神经。第二组：切断坐骨神经，随即在手术显微镜下用10／0无创伤缝合线缝合神经外膜以修复切断的坐骨神经。第三组：切除坐骨神经1cm。第四组：切除坐骨神经1cm，随即用第三组切除之坐骨神经行同种异体神经移植。第五组：切除坐骨神经1cm，随即将切除之坐骨神经原位缝合神经外膜修复坐骨神经，模拟自体神经移植。第六组(假手术对照组)：参照以上各组手术方法显露坐骨神经但不损伤。以上各组大鼠术后每一组随机分为5组，每组5只，分别于术后24小时、48小时、1周、2周、32天处死。经左心室—升主动脉插管灌注固定，然后迅速取出相应节段脊髓，将取出的脊髓多聚甲醛固定、脱水、浸蜡、包埋、切片、免疫组织化学处理，光镜观察，并对脊髓前角运动神经元行光密度测定。
结果：光镜下可见，Nogo-A在脊髓前角运动神经元胞浆内有明显
坐骨神经损伤后Nog，A在相应脊翻节段的表达
表达，而细胞核内未见表达。平均光密度测定显示，坐骨神经切断、
切除、切除后自体神经移植组及异体神经移植组脊髓灰质前角坐骨神
经损伤侧Nogo一A表达阳性的运动神经元平均光密度较未损伤侧明显
增高。其中，坐骨神经切断、切除及切除后自体神经移植组，相应损
伤侧脊髓前角Nogo一A表达阳性的运动神经元平均光密度增强的规律
基本相似，于伤后1、2天即出现增高，l周时达高峰，之后逐渐降低，
32天时趋向正常。坐骨神经切断后立即修复组，相应损伤侧脊髓前角
Nogo一A表达阳性的运动神经元平均光密度较未损伤侧无明显增强，且
各观察时间点平均光密度无明显差别、而坐骨神经切除后行异体神经
移植组，术后第1、2天相应损伤侧脊髓前角Nog。一A表达阳性的运动
神经元平均光密度即较未损伤侧明显增高，1周时增高幅度减小，2周
时达高峰，后虽逐渐降低，但至32天时仍处于较高水平。
结论:坐骨神经损伤后，脊髓相应节段损伤侧前角运动神经元胞
浆内Nogo一A的表达较未损伤侧明显增强。伤后立即行端一端吻合术修
复，脊髓相应节段损伤侧前角运动神经元胞浆内Nog。一A的表达基本
正常，无明显表达增强改变。伤后立即行自体神经移植术，脊髓相应
节段损伤侧前角运动神经元胞浆内Nogo一A的表达变化规律与不治疗
组相同;而立即行异体神经移植术，早期即可加重Nogo一A表达的增强，
在术后32天Nogo一A表达的增强仍维持于较高水平。
Objectives: To investigate the regularity of the expression of Nogo-A in spinal cord corresponding to traumatic sciatic nerve in the course of injury and repair.
Methods: 150 healthy adult Sprague Dawley(SD)rats were randomly divided into 6 groups, 25 per group. Group 1: sciatic nerves were severed without repair; Group 2: sciatic nerves were cut followed by immediate anastomosis of nerval adventitia using 10/0 non-traumatic suture; Group 3: 1 cm of sciatic nerve was resected in every subjects; Group 4: 1 cm resection of sciatic nerve was replaced by the resected sciatic nerve in group 3; Group 5: 1 cm of sciatic nerve was severed followed by epineurial suture in situ; Group 6: Visualization of sciatic nerve was performed without injury to it. The rats of each group were randomly divided into 5 subgroups, 5 per subgroup. The five subgroups were sacrificed at different intervals, 1 day, 2 day, 1 week, 2 week, 32day after operation. The expression of Nogo-A in spinal cord were detected by immunohistochemical staining and quantified by mean optical density (MOD).
Results: The expression of Nogo-A was positive in the cytochylema of motoneuron in anterior gray column of spinal cord. The MOD of traumatic side was evidently higher than non-traumatic side in group 1, 3, 5 at 1, 2 day after operation. The MOD of the severed side reached the top at 1 week.and then descented gradually, and almost normal at 32
days after operation. The MOD of traumatic side was not statistically different from non-traumatic side in the group 2. The MOD of traumatic side raised remarkable at 1 ,2 day after operation in the group of altogeneic transplantation, descented a little at 1 week, reach top at 2 week and maintained high level afterwards.
Conclusions: The expression of Nogo-A in motoneuron on traumatic side was higher than that on non-traumatic side. The expression of Nogo-A in traumatic side was different from non-traumatic side and came to normal on the condition that the traumatic nerve was immediately repaired by end-end anastomosis after injury. If the the traumatic nerve was replaced by allogeneic transplantation, the expression of Nogo-A in traumatic side were remarkable higher than that in non-traumatic side, and still maintained high level even after 1 month.
方法：选用健康Sprague Dawley(SD)大鼠150只，体重约200克，雌雄不拘，按照实验目的随机分为6组，每组25只。第一组：切断坐骨神经。第二组：切断坐骨神经，随即在手术显微镜下用10／0无创伤缝合线缝合神经外膜以修复切断的坐骨神经。第三组：切除坐骨神经1cm。第四组：切除坐骨神经1cm，随即用第三组切除之坐骨神经行同种异体神经移植。第五组：切除坐骨神经1cm，随即将切除之坐骨神经原位缝合神经外膜修复坐骨神经，模拟自体神经移植。第六组(假手术对照组)：参照以上各组手术方法显露坐骨神经但不损伤。以上各组大鼠术后每一组随机分为5组，每组5只，分别于术后24小时、48小时、1周、2周、32天处死。经左心室—升主动脉插管灌注固定，然后迅速取出相应节段脊髓，将取出的脊髓多聚甲醛固定、脱水、浸蜡、包埋、切片、免疫组织化学处理，光镜观察，并对脊髓前角运动神经元行光密度测定。
结果：光镜下可见，Nogo-A在脊髓前角运动神经元胞浆内有明显
坐骨神经损伤后Nog，A在相应脊翻节段的表达
表达，而细胞核内未见表达。平均光密度测定显示，坐骨神经切断、
切除、切除后自体神经移植组及异体神经移植组脊髓灰质前角坐骨神
经损伤侧Nogo一A表达阳性的运动神经元平均光密度较未损伤侧明显
增高。其中，坐骨神经切断、切除及切除后自体神经移植组，相应损
伤侧脊髓前角Nogo一A表达阳性的运动神经元平均光密度增强的规律
基本相似，于伤后1、2天即出现增高，l周时达高峰，之后逐渐降低，
32天时趋向正常。坐骨神经切断后立即修复组，相应损伤侧脊髓前角
Nogo一A表达阳性的运动神经元平均光密度较未损伤侧无明显增强，且
各观察时间点平均光密度无明显差别、而坐骨神经切除后行异体神经
移植组，术后第1、2天相应损伤侧脊髓前角Nog。一A表达阳性的运动
神经元平均光密度即较未损伤侧明显增高，1周时增高幅度减小，2周
时达高峰，后虽逐渐降低，但至32天时仍处于较高水平。
结论:坐骨神经损伤后，脊髓相应节段损伤侧前角运动神经元胞
浆内Nogo一A的表达较未损伤侧明显增强。伤后立即行端一端吻合术修
复，脊髓相应节段损伤侧前角运动神经元胞浆内Nog。一A的表达基本
正常，无明显表达增强改变。伤后立即行自体神经移植术，脊髓相应
节段损伤侧前角运动神经元胞浆内Nogo一A的表达变化规律与不治疗
组相同;而立即行异体神经移植术，早期即可加重Nogo一A表达的增强，
在术后32天Nogo一A表达的增强仍维持于较高水平。
Objectives: To investigate the regularity of the expression of Nogo-A in spinal cord corresponding to traumatic sciatic nerve in the course of injury and repair.
Methods: 150 healthy adult Sprague Dawley(SD)rats were randomly divided into 6 groups, 25 per group. Group 1: sciatic nerves were severed without repair; Group 2: sciatic nerves were cut followed by immediate anastomosis of nerval adventitia using 10/0 non-traumatic suture; Group 3: 1 cm of sciatic nerve was resected in every subjects; Group 4: 1 cm resection of sciatic nerve was replaced by the resected sciatic nerve in group 3; Group 5: 1 cm of sciatic nerve was severed followed by epineurial suture in situ; Group 6: Visualization of sciatic nerve was performed without injury to it. The rats of each group were randomly divided into 5 subgroups, 5 per subgroup. The five subgroups were sacrificed at different intervals, 1 day, 2 day, 1 week, 2 week, 32day after operation. The expression of Nogo-A in spinal cord were detected by immunohistochemical staining and quantified by mean optical density (MOD).
Results: The expression of Nogo-A was positive in the cytochylema of motoneuron in anterior gray column of spinal cord. The MOD of traumatic side was evidently higher than non-traumatic side in group 1, 3, 5 at 1, 2 day after operation. The MOD of the severed side reached the top at 1 week.and then descented gradually, and almost normal at 32
days after operation. The MOD of traumatic side was not statistically different from non-traumatic side in the group 2. The MOD of traumatic side raised remarkable at 1 ,2 day after operation in the group of altogeneic transplantation, descented a little at 1 week, reach top at 2 week and maintained high level afterwards.
Conclusions: The expression of Nogo-A in motoneuron on traumatic side was higher than that on non-traumatic side. The expression of Nogo-A in traumatic side was different from non-traumatic side and came to normal on the condition that the traumatic nerve was immediately repaired by end-end anastomosis after injury. If the the traumatic nerve was replaced by allogeneic transplantation, the expression of Nogo-A in traumatic side were remarkable higher than that in non-traumatic side, and still maintained high level even after 1 month.
引文
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