奶牛子宫内膜炎病原分离鉴定和子宫栓剂的研制
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摘要
为治疗奶牛子宫内膜炎,本试验以河北省邢台市3个大型牛场饲养的奶牛为调查对象,对20头患病奶牛子宫分泌物通过显微镜检查、细菌分离培养、生化鉴定等方法进行病原学研究。并在此基础上,通过子宫灌注致病性大肠杆菌、葡萄球菌、链球菌、沙门氏菌、蜡状芽孢杆菌等混合菌液,复制獭兔子宫内膜炎病理模型。运用正交试验的方法优选栓剂制备方案,以栓剂的色泽、不溶物、融变时限为标准,研制奶牛子宫内膜炎中药栓、复方碘栓和生物栓。对獭兔子宫内膜炎病理模型分别于第1d,3d,5d按0.2g/kg﹒bw子宫投放中药栓,复方碘栓,生物栓进行治疗,观察其用药前后的临床症状、子宫形态、子宫内膜病理学变化,并测定血清SOD,MDA,GSH-Px等生化指标。对30头患子宫内膜炎奶牛分别子宫投放三种栓剂,观察其用药前后临床症状,并于第0d、2d、4d、6d、8d测定血清SOD、GSH-Px活力和MDA含量。结果表明,从患病奶牛子宫分泌物中共检出6种病原菌,其中金黄色葡萄球菌8株(占34.78%)、表皮葡萄球菌6株(占26.09%)、链球菌2株(占8.70%)、沙门氏菌2株(占8.70%)、大肠杆菌4株(占17.40%)、蜡状芽孢杆菌1株(占4.35%)。制备出的中药栓,复方碘栓,生物栓符合药典标准。对獭兔子宫内膜炎病理模型治疗试验结果,与各组首次用药前各项指标相比,复方碘栓投药4d后,阴门肿胀及阴道充血逐渐消退,子宫分泌物逐渐减少,子宫颈和子宫角轻微肿胀,宫腔内有少量透明分泌物,子宫内膜较完整,上皮细胞结构基本正常,血清SOD和GSH-Px活力均显著增高(P<0.05);6d后血清SOD活力显著增高(P<0.05),而血清GSH-Px活力极显著增高(P<0.01),血清MDA含量极显著降低(P<0.01)。中药栓治疗组和生物栓治疗组临床症状均未有明显改善,血清SOD、GSH-Px活力和MDA含量变化也不明显。对30头患子宫内膜炎奶牛进行临床治疗试验结果,给药后患病奶牛阴门分泌物明显减少。第6d复方碘栓治疗组SOD活力极显著性增高(P<0.01),血清GSH-Px活力显著性增高(P<0.05),血清MDA含量极显著性降低(P<0.01)。第8d生物栓治疗组血清SOD活力显著性增高(P<0.05),血清GSH-Px活力极显著性增高(P<0.01),血清MDA含量极显著性降低(P<0.01);而中药栓治疗组血清GSH-Px活力显著性增高(P<0.05),MDA含量极显著性降低(P<0.01)。可见,复方碘栓是治疗子宫内膜炎的最佳药物。
In order to treat the cow endometritis, 20 endometritis cows in 3 large farms of XingTai, Hebei province were selected, their uterus secretions were studied by microscopic examination, isolation and culture of pathogens, biochemical identification etc. Under this foundation, the rabbit endometritis pathological model was made by perfusion of pathogenic E. coli, staphylococcus, streptococcus, Salmonella, Bacillus cereus and other mixed bacilli to the uterus. The schemes of the suppository preparation were optimized by orthogonal test, the color, insoluble substance, melt time were used as standard to prepare the traditional Chinese medicine suppository, the compound iodine suppository and the biological suppository. The suppositories were put to the uterus by 0.2g/kg﹒bw in 1st, 3rd, 5th days respectively, the clinical symptoms, uterine shape, pathological changes of endometrium, SOD, MDA, GSH-Px in sera, and other biochemical parameters were observed. The suppositories were put into the uterus of 30 endometritis cows respectively. Clinical symptoms were observed, and serum SOD, MDA, GSH-Px were examined in 0d, 2d, 4d, 6d, 8d treatment.The results showed that 6 pathogens were isolated in the uterus secretions, they were Staphylococcus aureus(8, 34.78%), Staphylococcus epidermidis(6, 26.09%), Streptococcus(2, 8.70%), Salmonella(2, 8.70%), E coli(4, 17.40%), Bacillus cereus(1, 4.35%) respectively. The traditional Chinese medicine suppository, the compound iodine suppository and the biological suppository were fit the standard of the pharmacopoeia. Compared with the indexes before using the suppositories, the results of rabbit endometritis pathological model after using the suppositories showed that the vulva edema and vaginal congestion subsided gradually, the uterine secretions reduced gradually. The uterine cervix and horn swelled slightly, there was a small amount of clear secretions in the uterus. The endometrium was more integrity, the structure of epithelial cell was normal. SOD and GSH-Px activity in sera were increased significantly (P<0.05) by 4 days treatment of compound iodine suppository. Serum SOD activity was increased significantly (P<0.05), serum GSH-Px activity was increased very significantly (P <0.01), serum MDA content was decreased very significantly (P <0.01) by 6 days treatment of compound iodine suppository. There were no significant change of the clinical symptoms, the serum SOD, GSH-Px and MDA in the traditional Chinese medicine suppository and biological suppository groups. The results on cow endometritis after using the suppositories showed that the uterine secretions of cows were obviously decreased. In 6d treatment, serum SOD activity increased significantly(P<0.01), serum GSH-Px activity increased significantly(P<0.05), and serum MDA content decreased significantly (P<0.01) in the compound iodine suppository groups. In 8d treatment, serum SOD activity increased significantly (P<0.05), serum GSH-Px activity increased significantly(P<0.01), and serum MDA content decreased significantly(P<0.01) in the biological suppository groups. While serum GSH-Px activity increased significantly (P<0.05) and serum MDA content decreased significantly (P<0.01) in the traditional Chinese medicine suppository groups. The compound iodine suppository was optimized in the suppositories for treatment of endometritis.
In order to treat the cow endometritis, 20 endometritis cows in 3 large farms of XingTai, Hebei province were selected, their uterus secretions were studied by microscopic examination, isolation and culture of pathogens, biochemical identification etc. Under this foundation, the rabbit endometritis pathological model was made by perfusion of pathogenic E. coli, staphylococcus, streptococcus, Salmonella, Bacillus cereus and other mixed bacilli to the uterus. The schemes of the suppository preparation were optimized by orthogonal test, the color, insoluble substance, melt time were used as standard to prepare the traditional Chinese medicine suppository, the compound iodine suppository and the biological suppository. The suppositories were put to the uterus by 0.2g/kg﹒bw in 1st, 3rd, 5th days respectively, the clinical symptoms, uterine shape, pathological changes of endometrium, SOD, MDA, GSH-Px in sera, and other biochemical parameters were observed. The suppositories were put into the uterus of 30 endometritis cows respectively. Clinical symptoms were observed, and serum SOD, MDA, GSH-Px were examined in 0d, 2d, 4d, 6d, 8d treatment.The results showed that 6 pathogens were isolated in the uterus secretions, they were Staphylococcus aureus(8, 34.78%), Staphylococcus epidermidis(6, 26.09%), Streptococcus(2, 8.70%), Salmonella(2, 8.70%), E coli(4, 17.40%), Bacillus cereus(1, 4.35%) respectively. The traditional Chinese medicine suppository, the compound iodine suppository and the biological suppository were fit the standard of the pharmacopoeia. Compared with the indexes before using the suppositories, the results of rabbit endometritis pathological model after using the suppositories showed that the vulva edema and vaginal congestion subsided gradually, the uterine secretions reduced gradually. The uterine cervix and horn swelled slightly, there was a small amount of clear secretions in the uterus. The endometrium was more integrity, the structure of epithelial cell was normal. SOD and GSH-Px activity in sera were increased significantly (P<0.05) by 4 days treatment of compound iodine suppository. Serum SOD activity was increased significantly (P<0.05), serum GSH-Px activity was increased very significantly (P <0.01), serum MDA content was decreased very significantly (P <0.01) by 6 days treatment of compound iodine suppository. There were no significant change of the clinical symptoms, the serum SOD, GSH-Px and MDA in the traditional Chinese medicine suppository and biological suppository groups. The results on cow endometritis after using the suppositories showed that the uterine secretions of cows were obviously decreased. In 6d treatment, serum SOD activity increased significantly(P<0.01), serum GSH-Px activity increased significantly(P<0.05), and serum MDA content decreased significantly (P<0.01) in the compound iodine suppository groups. In 8d treatment, serum SOD activity increased significantly (P<0.05), serum GSH-Px activity increased significantly(P<0.01), and serum MDA content decreased significantly(P<0.01) in the biological suppository groups. While serum GSH-Px activity increased significantly (P<0.05) and serum MDA content decreased significantly (P<0.01) in the traditional Chinese medicine suppository groups. The compound iodine suppository was optimized in the suppositories for treatment of endometritis.
引文
[1]Wsthes D C, Cheng Z, Chowdhury W, Fenwick M A, Fitzpatrick R, Moris D G, Patton J J. Negative energy balance alters global gene expression and immune responses in the uterus of postpartum dairy cows. Physiological Genomics, 2009, 39(1):1-13.
[2]Herath S, Lilly S T, Santos N R, Gilbert R O, Goetze L, Bryant C E, White J O, Cronin J, Sheldon I M. Expression of genes associated with immunity in the endometrium of cattle with disparate postpartum uterine disease and fertility. Reproductive Biology and Endocrinology, 2009 ,7: 5.
[3]Donofrio G, Ravanetti L, Cavirani S, Capocefalo A, Sheldon I M. Bacterial infection of endometrial stromal cells influences bovine herpesvirus 4 immediate early gene activation:a new insight into bacterial and viral interaction for uterine. Reproduction, 2008, 136(3): 361-366.
[4]Lee J I, Kim I H. Pregnancy loss in dairy cows: the contributing factors, the effects on reproductive performance and economic impact. Journal of Veterinary Science, 2007, 8(3): 283-288.
[5]Lincke A, Drillich M, Heuwieser W.Subclinical endometritis in dairy cattle and its effect on fertility-a review of recent publications. Berl Munch Tierarztl Wochenschr. 2007, 120(5-6): 245-50.
[6]牛建荣,杨国林,熊三友,等.母牛不孕症的病因概述[J].中兽医医药杂志, 1998, (4):68-69.
[7]李世平.奶牛子宫内膜炎常见病菌对清宫液的药物敏感试验[J].中兽医医药杂志, 1988,(3):12-14.
[8]陈家璞.乳牛疾病学[M].北京:农业出版社,1992,270-278.
[9]赵德明译.奶牛疾病学[M].北京:中国农业大学出版社,1999,427-438.
[10]徐耀基.微生态制剂治疗子宫内膜炎[J].动物医学进展,1998,19(2):35-36.
[11]瞿自明,熊三友,宋富源,等.清宫液治疗奶牛子宫内膜炎性不孕症的研究报告[J].中兽医医药杂志,1989,(6):1-6.
[12]吴明楼,刘士喜,苏辉,等.奶牛子宫内膜炎分泌物病原菌和病理学探讨[J].安徽农业科学, 2003,31(l):106-107. [13 ]岳春旺,孙茂红,段刚,等.奶牛子宫内膜炎综述[J].中国草食动物,2004,24(2):44-45.
[14]许微微,侯振中.奶牛子宫内膜炎厌氧菌分离鉴定[J].黑龙江畜牧兽医,2006, (6):73-74.
[15]陈星,胡松华,张克春,等.奶牛子宫内膜炎的病原菌的分离鉴定及药敏试验[J].中国兽医杂志,2010,46 (1):43-44.
[16]李洛平,张咏梅,李德竹.奶牛子宫内膜炎的病原菌的分离鉴定及药敏试验[J].当代畜牧,2008, (4):19-20.
[17]郭世宁,佟恒敏,刘远飞.11种中药及其复方制剂对奶牛子宫内膜炎的病原菌的体外抑菌实验观察[J].中国兽医杂志,2001,37(9):29-30.
[18]孔祥峰,刘志刚.南京某奶牛场子宫内膜炎病原菌分离鉴定及药敏试验[J].中国兽医杂志, 2005,41(8):24-25.
[19]杨克礼,潘玲,章孝荣,等.奶牛子宫内膜炎病原菌分离试验[J].中国奶牛,2006, 8:39-41.
[20]Takacs, T et al.Bacterial contamination of the uterus after parturition and its effect on the reproductive performance of cows on large-scale dairy farms.Theriogenology.1990, 33(4):851-866.
[21]Lioyd Elliontt et al.Uterus of the cow after Parturition.Bacterial content. 1998, (1): 77-81.
[22]杜光波,徐莉,高梦宁.奶牛子宫内膜炎主要病原菌的调查[J].畜牧与兽医,2007,39(4):24-26.
[23]王世荣,庄文忠,郁心连.奶牛子宫内膜炎病原菌检验[J].中国兽医科技, 1986(2):39-40.
[24]Michael D.whitacre.子宫灌注法在产后奶牛中的应用[J].畜禽疾病, 1993,14(1):30-33.
[25]许薇薇,侯振中,王海峰.奶牛子宫内膜炎厌氧菌的分离鉴定[J].黑龙江畜牧兽医,2006,(6):73-74.
[26] Miller AN, Williams EJ, Sibley K.The effects of Arcanobacterium pyogenes on endometrial function in vitro, and on uterine and ovarian function in vivo. Theriogenology. 2007, 68 (7):972-80.
[27] Donofrio G, Herath S, Sartori C. Bovine herpesvirus 4 is tropic for bovine endometrial cells and modulates endocrine function. Reproduction. 2007, 134 (1): 183-97.
[28] Fábián K, Makrai L, Sachse K.An investigation of the aetiological role of bovine herpesvirus 4 in bovine endometritis.Vet J. 2008 ,177(2):289-92.
[29]张桥,彭本英,金巍.奶牛慢性子宫内膜炎的治疗试验[J].湖北农学院学报, 2003, 23(5):331-333.
[30]Mishra V.K.,Sushrut Z.,Basanti B.Isolation of bacteria from the genital tract of cattle and buffaloes of gorakhpur district and their antibiogram[J].Indian Journal of Veterinary Medicine, 2005,25(1):43.
[31]Zahid Z.A.Culture and sensitivity of bacterial growth from exotic cows suffering from endometritis under Pakistani conditions[J].PakistanVeterinary Journal, 2004, 24(2): 107-108.
[32]Madhumeet S.,Satish K.,Shweta S.Study on the clinical efficacy of ciprofloxacin administered through intrauterine route in repeat breeder cows suffering from endometritis in Himachal Pradesh[J].Intas Polivet,2004,5(2):209-210.
[33]Kasimanickam R.,Duffield T.F.,Foster R.A.The effect of a single administration of cephapirin or cloprostenol on the reproductive performance of dairy cows with subclinical endometritis[J].Theriogenology,2005,63(3):818-830.
[34]Sandhu T.S.Clinical efficacy of various intrauterine preparations against post- parturient endometritis[J].Indian Veterinary Journal,2006,83(7):743-744.
[35]Ahmadi M.R.,Nazifi S.,Ghaisari H.R.The effect of intrauterine cephapirin on treatment of endometritis in commercial dairy cattle[J]. Archives of Razi Institute,2005,59:35-45.
[36]Sheldon I.M.,Bushnell M.,Montgomery J.et al.Minimum inhibitory concentrations of some antimicrobial drugs against bacteria causing uterine infections in cattle[J]. Veterinary Record, 2004,155(13):383-387.
[37]高世涛,宋卫东.强奶源管理正确使用有效检测实现牛奶抗生素残留达到安全极限[J].中国乳业,2002,8(10)12-14.
[38]BretzlaffK.Rational effort treatment of endometritis in the dairy cow[J].Vet Clinical North Am Food Animal Pract,1987,3:593-607.
[39]时维静,立顺.牛子宫内膜炎的治疗[J].中国奶牛,2004,2(6):42-44.
[40]汤春华,礼中,建清,.草药制剂防治奶牛繁殖障碍疾病的效果[J].江苏农业科学,2001, (1):61-62.
[41]王建发,刘兰,张鹏宇.防治奶牛子宫内膜炎中药复方药理实验研究[J].中兽医学杂志,2008, (5):11-13.
[42]钟建新,陈仲国,聂建超.益母生化散的临床应用及毒副作用观察试验[J].兽药与饲料添加剂,2003,6(8):22.
[43]李呈敏.纯中药“孕宝”冲剂治疗母牛子宫内膜炎性不孕症的疗效观察[J].乳业科学与技术.2000.(4):39-42.
[44]常玉军,王丽梅,常玉臣,等.用清宫消炎混悬剂治疗奶牛子宫内膜炎试验报告[J].中国草食动物,2005, (1):50.
[45]何海健,兰新财.宫炎消栓防治奶牛子宫内膜炎的疗效观察[J].中兽医学杂志.2007, (3):3-5.
[46]弓素梅,倪耀娣,郭红斌.治疗奶牛子宫内膜炎益母生化栓的研究[J].湖北畜牧兽医,2006, 7(7):19-21.
[47]Singla P.,Jagir S.,Sharma N.S.Effect of post AI immunotherapy on dynamics of uterine microflora and conception in subclinical endometritis cow[J].Indian Journal of Animal Science.2004,74(7):706-709.
[48]Methai A.,Rajasundaram R.C.,Veerapandian C.Intrauterine plasma treatment for endometritis in Jersey crossbred cow[J].Indian Journal of Animal Reproduction. 2005, 26(1): 7-10.
[49]陈杖榴.兽医药理学(第二版)[M].北京:中国农业出版社,2002,1:140-163.
[50]Sheldon I.M.,Noakes D.E.,Rycroft A.N.,et al.Effect of intrauterine administration of oestradiol on postpartum uterinebacterial infection in cattle[J].Animal ReproductionScience.2004,81(1-2):13-23.
[51]Marc D,Damaris R,Miriam W,et al.Treatment of chronic endometritis in dairy cows with an intrauterine application of enzymes A field trial[J]. Theriogenology. 2005,63,(7):1811-1823.
[52]Drillich M.An update on uterine infections in dairy cattle[J].Slovenian Veterinary Research.2006,43(1):11-15.
[53]彭招华,袁侣明,韩民利.丹参的药理作用研究概况[J].中药材,2001,24(9):690-693.
[54]Sakurai H,Adams B M et al.Concentration of gonadotropin-releasing hormone receptor messenger ribonucleic acid inpituitary tissue of orchidectomized sheep:effect of passiveimmunization gaaist gonadotropin-releasing hormone.Ani-mal Breeding abstracts,1997.65(7):522.
[55]唐海燕.奶牛子宫内膜炎中药配方的研究[D].内蒙古农大.2004.
[56]周燕,何效平.高效杀菌剂-聚维酮碘[J]中国临床医药实用杂志,2004,19(2):144.
[57]周建标,周云拍,王之法.聚维酮碘在消毒和抗感染中的应用[J].药学实践杂志,1999,17(5):271-273.
[58]黄艳,郑越平,胡云,等.聚维酮碘杀菌效果的试验观察[J].医学动物防制, 2004, 20(4):196-198.
[59]李建平,赵青,温悦,等.聚维酮碘剂型进展及临床应用[J].中国药业, 2002, 11(3): 78-79.
[60]江长波.中药栓剂的发展与应用概况[J].江西中医药,2001,5(5):45.
[61]刘佳丽,王地,杨世英.栓剂的药剂学研究进展(综述)[J].北京中医药大学学报,2000,6(1):71.
[62]范碧亭.中药药剂学[M].上海:上海科学技术出版社,1997.
[63]毕殿洲.药剂学[Ml.北京:人民卫生出版社,1998.
[64]王炯.中药栓剂在制备中应注意的几个问题[J].甘肃中医,2000,4(4):50.
[65]刘保良.李军乎.郭鸿.栓剂的研究开发[J].中国医药工业杂志,1994,25(6):283.
[66]莫伟伟.新型奶牛子宫内膜炎中药复方的研制[D].内蒙古农业大学.2006.
[67]陈品球,蒋继琰.中药子宫灌注液治疗奶牛子宫内膜炎的效果观察[J].中兽医学杂志,2006, (4):21-22.
[68]张义冉,汪恩强,秦建华,等.制备奶牛子宫内膜炎中药栓剂的优选方案研究[J].中国奶牛,2009,(7):28.
[69]栾海云,张树平,李淑翠.聚维酮碘溶液的制备[J].滨州医学院学报,2007,30(4):297.
[70]王晓颖,侯振中,赵树臣.脂多糖治疗子宫内膜炎的方法与传统疗法的比较[J].养殖与顾问,2007,(4):55.
[71]Singla P., Jagir S., Sharma N. S. Effect of post AI immunotherapy on dynamics of uterine microflora and conception in subclinical endometritis cow[J]. Indian Journal of Animal Science.2004,74(7):706-709.
[72]葛利江,田文儒,宣长和.母牛子宫内膜炎的免疫调控[J].黑龙江畜牧兽医, 2001, (9):31-33.
[73]刘水平,邬国军著.医学微生物学实验指导[M].西安:世界图书出版社西安公司.2002,4.
[74]周庭银,赵虎编著.临床微生物学诊断与图解[M].上海:上海科学技术出版社.2001.
[75]东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001:77-78.
[76]方开泰,马长兴.正交与均匀试验设计[M].北京:科学出版社,2001.
[77]王炯.中药栓剂在制备中应注意的几个问题[J].甘肃中医.2000,(4):56.
[78]张义冉,汪恩强.河北省部分奶牛场奶牛子宫内膜炎病原菌分离与鉴定[J].畜牧与兽医,2009,41(5):86-88.
[79]孔祥峰,胡元亮,黄小燕,等.新促孕液及其加味方治疗子宫内膜炎的试验研究[J].畜牧兽医学报.2006,37(4):379-383.
[80]赵兴绪.家畜产科学(第3版)[M].北京:中国农业出版社.2002,376-378.
[81]王建林,刘绪川,王宇一,等.奶牛子宫内膜炎实验动物模型复制及其病理组织变化观察[J].中兽医医药杂志.1992,(6):5-7.
[82]李甘地.组织病理技术[M].北京:人民卫生出版社.2002,1-6.
[83]刘红梅,戴宝贵,郝洪伟,等.奶牛子宫内膜炎的病因分析与治疗[J].畜牧兽医科技信息,2005,8(5):51.
[84]赵建英,张泽恩,越永波.金乳康治疗奶牛乳房炎,子宫内膜炎效果综述[J].中国奶牛,2003,3(2):21-24.
[85]司建兵,魏晓燕,陈继荣,等.奶牛子宫炎的临床调查与诊治[J].乳业科学与技术,2005,2(2):90-91.
[86]王宗保,姚峰,吴端生,等.高糖高脂致家兔糖尿病的实验模型探讨[J].中国实验动物学报,2003,11(2):103-106.
[87]孔祥峰,胡元亮,郭振环,等.兔子宫内膜炎模型的建立和临床病理学观察[J].中国实验动物学报,2006,14(3):213-216.
[88]邢震全,王力波,张彦春.奶牛子宫内膜炎的治疗[J].黑龙江动物繁殖, 2001 ,9(4):39-40.
[89]张辉,姚克.聚维酮碘在眼科感染防治中的应用[J].国外医学(眼科学分册), 2005, (4):4-7.
[90]莫莉.聚维酮碘阴道泡腾片和复方聚维酮碘凝胶的研制及应用研究[D].华中农业大学,2006.
[2]Herath S, Lilly S T, Santos N R, Gilbert R O, Goetze L, Bryant C E, White J O, Cronin J, Sheldon I M. Expression of genes associated with immunity in the endometrium of cattle with disparate postpartum uterine disease and fertility. Reproductive Biology and Endocrinology, 2009 ,7: 5.
[3]Donofrio G, Ravanetti L, Cavirani S, Capocefalo A, Sheldon I M. Bacterial infection of endometrial stromal cells influences bovine herpesvirus 4 immediate early gene activation:a new insight into bacterial and viral interaction for uterine. Reproduction, 2008, 136(3): 361-366.
[4]Lee J I, Kim I H. Pregnancy loss in dairy cows: the contributing factors, the effects on reproductive performance and economic impact. Journal of Veterinary Science, 2007, 8(3): 283-288.
[5]Lincke A, Drillich M, Heuwieser W.Subclinical endometritis in dairy cattle and its effect on fertility-a review of recent publications. Berl Munch Tierarztl Wochenschr. 2007, 120(5-6): 245-50.
[6]牛建荣,杨国林,熊三友,等.母牛不孕症的病因概述[J].中兽医医药杂志, 1998, (4):68-69.
[7]李世平.奶牛子宫内膜炎常见病菌对清宫液的药物敏感试验[J].中兽医医药杂志, 1988,(3):12-14.
[8]陈家璞.乳牛疾病学[M].北京:农业出版社,1992,270-278.
[9]赵德明译.奶牛疾病学[M].北京:中国农业大学出版社,1999,427-438.
[10]徐耀基.微生态制剂治疗子宫内膜炎[J].动物医学进展,1998,19(2):35-36.
[11]瞿自明,熊三友,宋富源,等.清宫液治疗奶牛子宫内膜炎性不孕症的研究报告[J].中兽医医药杂志,1989,(6):1-6.
[12]吴明楼,刘士喜,苏辉,等.奶牛子宫内膜炎分泌物病原菌和病理学探讨[J].安徽农业科学, 2003,31(l):106-107. [13 ]岳春旺,孙茂红,段刚,等.奶牛子宫内膜炎综述[J].中国草食动物,2004,24(2):44-45.
[14]许微微,侯振中.奶牛子宫内膜炎厌氧菌分离鉴定[J].黑龙江畜牧兽医,2006, (6):73-74.
[15]陈星,胡松华,张克春,等.奶牛子宫内膜炎的病原菌的分离鉴定及药敏试验[J].中国兽医杂志,2010,46 (1):43-44.
[16]李洛平,张咏梅,李德竹.奶牛子宫内膜炎的病原菌的分离鉴定及药敏试验[J].当代畜牧,2008, (4):19-20.
[17]郭世宁,佟恒敏,刘远飞.11种中药及其复方制剂对奶牛子宫内膜炎的病原菌的体外抑菌实验观察[J].中国兽医杂志,2001,37(9):29-30.
[18]孔祥峰,刘志刚.南京某奶牛场子宫内膜炎病原菌分离鉴定及药敏试验[J].中国兽医杂志, 2005,41(8):24-25.
[19]杨克礼,潘玲,章孝荣,等.奶牛子宫内膜炎病原菌分离试验[J].中国奶牛,2006, 8:39-41.
[20]Takacs, T et al.Bacterial contamination of the uterus after parturition and its effect on the reproductive performance of cows on large-scale dairy farms.Theriogenology.1990, 33(4):851-866.
[21]Lioyd Elliontt et al.Uterus of the cow after Parturition.Bacterial content. 1998, (1): 77-81.
[22]杜光波,徐莉,高梦宁.奶牛子宫内膜炎主要病原菌的调查[J].畜牧与兽医,2007,39(4):24-26.
[23]王世荣,庄文忠,郁心连.奶牛子宫内膜炎病原菌检验[J].中国兽医科技, 1986(2):39-40.
[24]Michael D.whitacre.子宫灌注法在产后奶牛中的应用[J].畜禽疾病, 1993,14(1):30-33.
[25]许薇薇,侯振中,王海峰.奶牛子宫内膜炎厌氧菌的分离鉴定[J].黑龙江畜牧兽医,2006,(6):73-74.
[26] Miller AN, Williams EJ, Sibley K.The effects of Arcanobacterium pyogenes on endometrial function in vitro, and on uterine and ovarian function in vivo. Theriogenology. 2007, 68 (7):972-80.
[27] Donofrio G, Herath S, Sartori C. Bovine herpesvirus 4 is tropic for bovine endometrial cells and modulates endocrine function. Reproduction. 2007, 134 (1): 183-97.
[28] Fábián K, Makrai L, Sachse K.An investigation of the aetiological role of bovine herpesvirus 4 in bovine endometritis.Vet J. 2008 ,177(2):289-92.
[29]张桥,彭本英,金巍.奶牛慢性子宫内膜炎的治疗试验[J].湖北农学院学报, 2003, 23(5):331-333.
[30]Mishra V.K.,Sushrut Z.,Basanti B.Isolation of bacteria from the genital tract of cattle and buffaloes of gorakhpur district and their antibiogram[J].Indian Journal of Veterinary Medicine, 2005,25(1):43.
[31]Zahid Z.A.Culture and sensitivity of bacterial growth from exotic cows suffering from endometritis under Pakistani conditions[J].PakistanVeterinary Journal, 2004, 24(2): 107-108.
[32]Madhumeet S.,Satish K.,Shweta S.Study on the clinical efficacy of ciprofloxacin administered through intrauterine route in repeat breeder cows suffering from endometritis in Himachal Pradesh[J].Intas Polivet,2004,5(2):209-210.
[33]Kasimanickam R.,Duffield T.F.,Foster R.A.The effect of a single administration of cephapirin or cloprostenol on the reproductive performance of dairy cows with subclinical endometritis[J].Theriogenology,2005,63(3):818-830.
[34]Sandhu T.S.Clinical efficacy of various intrauterine preparations against post- parturient endometritis[J].Indian Veterinary Journal,2006,83(7):743-744.
[35]Ahmadi M.R.,Nazifi S.,Ghaisari H.R.The effect of intrauterine cephapirin on treatment of endometritis in commercial dairy cattle[J]. Archives of Razi Institute,2005,59:35-45.
[36]Sheldon I.M.,Bushnell M.,Montgomery J.et al.Minimum inhibitory concentrations of some antimicrobial drugs against bacteria causing uterine infections in cattle[J]. Veterinary Record, 2004,155(13):383-387.
[37]高世涛,宋卫东.强奶源管理正确使用有效检测实现牛奶抗生素残留达到安全极限[J].中国乳业,2002,8(10)12-14.
[38]BretzlaffK.Rational effort treatment of endometritis in the dairy cow[J].Vet Clinical North Am Food Animal Pract,1987,3:593-607.
[39]时维静,立顺.牛子宫内膜炎的治疗[J].中国奶牛,2004,2(6):42-44.
[40]汤春华,礼中,建清,.草药制剂防治奶牛繁殖障碍疾病的效果[J].江苏农业科学,2001, (1):61-62.
[41]王建发,刘兰,张鹏宇.防治奶牛子宫内膜炎中药复方药理实验研究[J].中兽医学杂志,2008, (5):11-13.
[42]钟建新,陈仲国,聂建超.益母生化散的临床应用及毒副作用观察试验[J].兽药与饲料添加剂,2003,6(8):22.
[43]李呈敏.纯中药“孕宝”冲剂治疗母牛子宫内膜炎性不孕症的疗效观察[J].乳业科学与技术.2000.(4):39-42.
[44]常玉军,王丽梅,常玉臣,等.用清宫消炎混悬剂治疗奶牛子宫内膜炎试验报告[J].中国草食动物,2005, (1):50.
[45]何海健,兰新财.宫炎消栓防治奶牛子宫内膜炎的疗效观察[J].中兽医学杂志.2007, (3):3-5.
[46]弓素梅,倪耀娣,郭红斌.治疗奶牛子宫内膜炎益母生化栓的研究[J].湖北畜牧兽医,2006, 7(7):19-21.
[47]Singla P.,Jagir S.,Sharma N.S.Effect of post AI immunotherapy on dynamics of uterine microflora and conception in subclinical endometritis cow[J].Indian Journal of Animal Science.2004,74(7):706-709.
[48]Methai A.,Rajasundaram R.C.,Veerapandian C.Intrauterine plasma treatment for endometritis in Jersey crossbred cow[J].Indian Journal of Animal Reproduction. 2005, 26(1): 7-10.
[49]陈杖榴.兽医药理学(第二版)[M].北京:中国农业出版社,2002,1:140-163.
[50]Sheldon I.M.,Noakes D.E.,Rycroft A.N.,et al.Effect of intrauterine administration of oestradiol on postpartum uterinebacterial infection in cattle[J].Animal ReproductionScience.2004,81(1-2):13-23.
[51]Marc D,Damaris R,Miriam W,et al.Treatment of chronic endometritis in dairy cows with an intrauterine application of enzymes A field trial[J]. Theriogenology. 2005,63,(7):1811-1823.
[52]Drillich M.An update on uterine infections in dairy cattle[J].Slovenian Veterinary Research.2006,43(1):11-15.
[53]彭招华,袁侣明,韩民利.丹参的药理作用研究概况[J].中药材,2001,24(9):690-693.
[54]Sakurai H,Adams B M et al.Concentration of gonadotropin-releasing hormone receptor messenger ribonucleic acid inpituitary tissue of orchidectomized sheep:effect of passiveimmunization gaaist gonadotropin-releasing hormone.Ani-mal Breeding abstracts,1997.65(7):522.
[55]唐海燕.奶牛子宫内膜炎中药配方的研究[D].内蒙古农大.2004.
[56]周燕,何效平.高效杀菌剂-聚维酮碘[J]中国临床医药实用杂志,2004,19(2):144.
[57]周建标,周云拍,王之法.聚维酮碘在消毒和抗感染中的应用[J].药学实践杂志,1999,17(5):271-273.
[58]黄艳,郑越平,胡云,等.聚维酮碘杀菌效果的试验观察[J].医学动物防制, 2004, 20(4):196-198.
[59]李建平,赵青,温悦,等.聚维酮碘剂型进展及临床应用[J].中国药业, 2002, 11(3): 78-79.
[60]江长波.中药栓剂的发展与应用概况[J].江西中医药,2001,5(5):45.
[61]刘佳丽,王地,杨世英.栓剂的药剂学研究进展(综述)[J].北京中医药大学学报,2000,6(1):71.
[62]范碧亭.中药药剂学[M].上海:上海科学技术出版社,1997.
[63]毕殿洲.药剂学[Ml.北京:人民卫生出版社,1998.
[64]王炯.中药栓剂在制备中应注意的几个问题[J].甘肃中医,2000,4(4):50.
[65]刘保良.李军乎.郭鸿.栓剂的研究开发[J].中国医药工业杂志,1994,25(6):283.
[66]莫伟伟.新型奶牛子宫内膜炎中药复方的研制[D].内蒙古农业大学.2006.
[67]陈品球,蒋继琰.中药子宫灌注液治疗奶牛子宫内膜炎的效果观察[J].中兽医学杂志,2006, (4):21-22.
[68]张义冉,汪恩强,秦建华,等.制备奶牛子宫内膜炎中药栓剂的优选方案研究[J].中国奶牛,2009,(7):28.
[69]栾海云,张树平,李淑翠.聚维酮碘溶液的制备[J].滨州医学院学报,2007,30(4):297.
[70]王晓颖,侯振中,赵树臣.脂多糖治疗子宫内膜炎的方法与传统疗法的比较[J].养殖与顾问,2007,(4):55.
[71]Singla P., Jagir S., Sharma N. S. Effect of post AI immunotherapy on dynamics of uterine microflora and conception in subclinical endometritis cow[J]. Indian Journal of Animal Science.2004,74(7):706-709.
[72]葛利江,田文儒,宣长和.母牛子宫内膜炎的免疫调控[J].黑龙江畜牧兽医, 2001, (9):31-33.
[73]刘水平,邬国军著.医学微生物学实验指导[M].西安:世界图书出版社西安公司.2002,4.
[74]周庭银,赵虎编著.临床微生物学诊断与图解[M].上海:上海科学技术出版社.2001.
[75]东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001:77-78.
[76]方开泰,马长兴.正交与均匀试验设计[M].北京:科学出版社,2001.
[77]王炯.中药栓剂在制备中应注意的几个问题[J].甘肃中医.2000,(4):56.
[78]张义冉,汪恩强.河北省部分奶牛场奶牛子宫内膜炎病原菌分离与鉴定[J].畜牧与兽医,2009,41(5):86-88.
[79]孔祥峰,胡元亮,黄小燕,等.新促孕液及其加味方治疗子宫内膜炎的试验研究[J].畜牧兽医学报.2006,37(4):379-383.
[80]赵兴绪.家畜产科学(第3版)[M].北京:中国农业出版社.2002,376-378.
[81]王建林,刘绪川,王宇一,等.奶牛子宫内膜炎实验动物模型复制及其病理组织变化观察[J].中兽医医药杂志.1992,(6):5-7.
[82]李甘地.组织病理技术[M].北京:人民卫生出版社.2002,1-6.
[83]刘红梅,戴宝贵,郝洪伟,等.奶牛子宫内膜炎的病因分析与治疗[J].畜牧兽医科技信息,2005,8(5):51.
[84]赵建英,张泽恩,越永波.金乳康治疗奶牛乳房炎,子宫内膜炎效果综述[J].中国奶牛,2003,3(2):21-24.
[85]司建兵,魏晓燕,陈继荣,等.奶牛子宫炎的临床调查与诊治[J].乳业科学与技术,2005,2(2):90-91.
[86]王宗保,姚峰,吴端生,等.高糖高脂致家兔糖尿病的实验模型探讨[J].中国实验动物学报,2003,11(2):103-106.
[87]孔祥峰,胡元亮,郭振环,等.兔子宫内膜炎模型的建立和临床病理学观察[J].中国实验动物学报,2006,14(3):213-216.
[88]邢震全,王力波,张彦春.奶牛子宫内膜炎的治疗[J].黑龙江动物繁殖, 2001 ,9(4):39-40.
[89]张辉,姚克.聚维酮碘在眼科感染防治中的应用[J].国外医学(眼科学分册), 2005, (4):4-7.
[90]莫莉.聚维酮碘阴道泡腾片和复方聚维酮碘凝胶的研制及应用研究[D].华中农业大学,2006.