北京地区奶牛子宫内膜炎致病菌的分离鉴定及抑菌试验
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摘要
奶牛子宫内膜炎是奶牛常见的产科疾病,可导致繁殖障碍,给奶牛养殖业造成很大的经济损失。
本试验选取了产后正常奶牛和患子宫内膜炎奶牛作为研究对象,采集北京地区奶牛场8头正常奶牛和60头患子宫内膜炎奶牛的子宫分泌物,分离鉴定了引起子宫内膜炎的主要致病菌。从产后0-30 d正常奶牛子宫中分离细菌,以产后2-7d检出的细菌数最多。从患子宫内膜炎奶牛子宫中分离到的主要致病菌为大肠杆菌、葡萄球菌、无乳链球菌、乳房链球菌、粪肠球菌、芽孢杆菌,这对进一步研究奶牛子宫内膜炎提供了参考。
为了研究中、西药对奶牛子宫内膜炎致病菌的敏感程度,本试验进行了抗生素、中药对子宫内膜炎主要致病菌的体外抑菌试验。结果表明,氨苄西林和环丙沙星对这些主要致病菌株有较强的抑制作用,而青霉素、链霉素等对这些致病菌抑制效果较差,但它们都出现了不同程度的抗药性。中药以金银花、连翘、蒲公英的抑菌效果较好。这为临床上合理地用药来治疗奶牛子宫内膜炎提供了重要依据。
本试验采用试管二倍稀释法,测定了中药对奶牛子宫内膜炎主要致病菌的抑菌效果。结果表明,由金银花、连翘、蒲公英等中草药组成的中药方剂对奶牛子宫内膜炎致病菌有显著的抑菌作用。通过体外抑菌试验选取效果好的组方应用于临床,来治疗奶牛子宫内膜炎。
体内试验,选取10头产后健康奶牛作为正常对照组,20头子宫内膜炎患牛随机分为用药组和未用药组,分别于用药前和用药后第3d、5d、7d颈静脉采血。通过临床药效学试验,测定超氧化物歧化酶(SOD)、丙二醛(MDA)含量等,观察中药方剂治疗奶牛子宫内膜炎的临床效果。结果表明：该中药方剂可提高奶牛血清SOD、GSH-Px活性,降低血清MDA含量,增强机体的抗氧化能力,减弱机体脂质过氧化程度,提高血清中IgG、IgA含量,增强子宫内膜炎奶牛的免疫功能。
结论：抗生素对分离的致病菌有一定的效果,但出现了不同程度的抗药性,而中药方剂对奶牛子宫内膜炎的治疗有良好的效果。
Cow endometritis is a common postpartum obstetric disease mainly caused by bacteria infections, which can result in reproductive obstacles and great economic loss in the dairy industry.
The uterie secretions of 8 postpartum healthy cows and 60 endometritis cows from Beijing region were collected, and the pathogenic bacteria were isolated and identificated. The bacteria in 0-30 d postpartum healthy cow uterus were isolated, the bacteria number was the most in 2-7d postpartum. The main pathogenic bacteria of endometritis cows were Escherichia coli, Staphylococcus, Streptococus agalactiae, Streptococus uberis, Enterococcus faecalis, Bacillus respectively. It provided a reference for treatment of cow endometritis.
In order to research the sensitive degree of the herbal medicine and the western drugs on cow endometritis pathogenic bacteria, the antibiotics and herbal medicine were used for in vitro bacteriostatic experiment of the main pathogenic bacteria in endometritis cows. The results showed that the bacteriostatic effects of Ampicillin and Ciprofloxacin were better, but those of Penicillin and Streptomycin were less. All of them showed drug resistance. The bacteriostatic effects of herbal medicine Honeysuckle, Forsythia, Taraxacum were better. It provided important basis for treatment of cow endometriosis in clinic.
The bacteriostatic effects of the herbal medicine were determined by 2-fold dilution method. The results showed that the herbal medicine prescription composed of Honeysuckle, Forsythia, Taraxacum etc had remarkable bacteriostatic effects for cow endometritis pathogenic bacteria. By bacteriostatic experiment in vitro, the herbal formula could be selected for dairy cow endometritis treatment.
In vivo experiment, the blood samples from 10 healthy dairy cows and 20 endometritis dairy cows before and after drug treatment were collected. SOD and MDA etc were measured by clinical pharmacodynamics test, to observe the clinical effect of the herbal formula on cow endometritis. The results showed this herbal formula could decrease the content of MDA, increase SOD and GSH-Px activities, enhance the contents of IgG and IgA, and improve the immunity of endometritis dairy cows.
Conclusion:The antibiotics had some bacteriostatic effects to the endometritis pathogenic bacteria, but showed drug resistance. The herbal formula showed better effect for treatment of cow endometriosis in clinic.
本试验选取了产后正常奶牛和患子宫内膜炎奶牛作为研究对象,采集北京地区奶牛场8头正常奶牛和60头患子宫内膜炎奶牛的子宫分泌物,分离鉴定了引起子宫内膜炎的主要致病菌。从产后0-30 d正常奶牛子宫中分离细菌,以产后2-7d检出的细菌数最多。从患子宫内膜炎奶牛子宫中分离到的主要致病菌为大肠杆菌、葡萄球菌、无乳链球菌、乳房链球菌、粪肠球菌、芽孢杆菌,这对进一步研究奶牛子宫内膜炎提供了参考。
为了研究中、西药对奶牛子宫内膜炎致病菌的敏感程度,本试验进行了抗生素、中药对子宫内膜炎主要致病菌的体外抑菌试验。结果表明,氨苄西林和环丙沙星对这些主要致病菌株有较强的抑制作用,而青霉素、链霉素等对这些致病菌抑制效果较差,但它们都出现了不同程度的抗药性。中药以金银花、连翘、蒲公英的抑菌效果较好。这为临床上合理地用药来治疗奶牛子宫内膜炎提供了重要依据。
本试验采用试管二倍稀释法,测定了中药对奶牛子宫内膜炎主要致病菌的抑菌效果。结果表明,由金银花、连翘、蒲公英等中草药组成的中药方剂对奶牛子宫内膜炎致病菌有显著的抑菌作用。通过体外抑菌试验选取效果好的组方应用于临床,来治疗奶牛子宫内膜炎。
体内试验,选取10头产后健康奶牛作为正常对照组,20头子宫内膜炎患牛随机分为用药组和未用药组,分别于用药前和用药后第3d、5d、7d颈静脉采血。通过临床药效学试验,测定超氧化物歧化酶(SOD)、丙二醛(MDA)含量等,观察中药方剂治疗奶牛子宫内膜炎的临床效果。结果表明：该中药方剂可提高奶牛血清SOD、GSH-Px活性,降低血清MDA含量,增强机体的抗氧化能力,减弱机体脂质过氧化程度,提高血清中IgG、IgA含量,增强子宫内膜炎奶牛的免疫功能。
结论：抗生素对分离的致病菌有一定的效果,但出现了不同程度的抗药性,而中药方剂对奶牛子宫内膜炎的治疗有良好的效果。
Cow endometritis is a common postpartum obstetric disease mainly caused by bacteria infections, which can result in reproductive obstacles and great economic loss in the dairy industry.
The uterie secretions of 8 postpartum healthy cows and 60 endometritis cows from Beijing region were collected, and the pathogenic bacteria were isolated and identificated. The bacteria in 0-30 d postpartum healthy cow uterus were isolated, the bacteria number was the most in 2-7d postpartum. The main pathogenic bacteria of endometritis cows were Escherichia coli, Staphylococcus, Streptococus agalactiae, Streptococus uberis, Enterococcus faecalis, Bacillus respectively. It provided a reference for treatment of cow endometritis.
In order to research the sensitive degree of the herbal medicine and the western drugs on cow endometritis pathogenic bacteria, the antibiotics and herbal medicine were used for in vitro bacteriostatic experiment of the main pathogenic bacteria in endometritis cows. The results showed that the bacteriostatic effects of Ampicillin and Ciprofloxacin were better, but those of Penicillin and Streptomycin were less. All of them showed drug resistance. The bacteriostatic effects of herbal medicine Honeysuckle, Forsythia, Taraxacum were better. It provided important basis for treatment of cow endometriosis in clinic.
The bacteriostatic effects of the herbal medicine were determined by 2-fold dilution method. The results showed that the herbal medicine prescription composed of Honeysuckle, Forsythia, Taraxacum etc had remarkable bacteriostatic effects for cow endometritis pathogenic bacteria. By bacteriostatic experiment in vitro, the herbal formula could be selected for dairy cow endometritis treatment.
In vivo experiment, the blood samples from 10 healthy dairy cows and 20 endometritis dairy cows before and after drug treatment were collected. SOD and MDA etc were measured by clinical pharmacodynamics test, to observe the clinical effect of the herbal formula on cow endometritis. The results showed this herbal formula could decrease the content of MDA, increase SOD and GSH-Px activities, enhance the contents of IgG and IgA, and improve the immunity of endometritis dairy cows.
Conclusion:The antibiotics had some bacteriostatic effects to the endometritis pathogenic bacteria, but showed drug resistance. The herbal formula showed better effect for treatment of cow endometriosis in clinic.
引文
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