三个绵羊品种及两个杂种后代的微卫星多态性研究
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摘要
本研究在“北方肉用绵羊新品种培育基地”采集无角陶赛特羊、小尾寒羊、蒙古羊、陶蒙杂交一代羊和陶寒杂交一代羊血液各50头份,经提取筛选出了理想的血液DNA样品各25个,采用微卫星标记法,选择了McM42、MAF65、OarFCB11、McM527、HSC、OarFCB20、OarAE101、RM0509、MNS0094、ILSTS004、BM1329、CSSM0004共12个微卫星位点,通过PCR扩增和聚丙烯酰胺凝胶电泳对其微卫星多态性进行了研究,结果表明:
对5个群体125只个体血液DNA样品的PCR产物进行了非变性聚丙烯酰胺凝胶电泳以及电泳图谱分析,结果共检测到198个等位基因,平均每个位点有16.5个等位基因,说明12个微卫星位点都具有较高的多态性。
通过计算等位基因频率、遗传杂合度、多态信息含量、Nei氏遗传距离和系统聚类分析,5个绵羊群体在不同位点的多态信息含量(PIC)普遍较高,基本上都在0.7以上;无角陶赛特、小尾寒羊和蒙古羊的平均杂合度分别为0.8642、0.8558、0.8425,两个杂交后代陶寒一代羊和陶蒙一代羊的平均杂合度分别为0.7900、0.7811;Nei氏标准遗传距离由远到近依次为无角陶赛特和小尾寒羊(0.2327)、无角陶赛特和蒙古羊(0.1859)、蒙古羊和小尾寒羊(0.1492),引进品种无角陶赛特和两个地方品种间的遗传距离绝对值都较大;结合非加权组对算术平均法(UPGMA)和邻近结合法(NJ)两种系统发育树的分析,无角陶赛特和小尾寒羊的亲缘关系最远,小尾寒羊和蒙古羊的亲缘关系较近。
从微卫星DNA分子水平进一步说明在北方肉用绵羊新品种培育中,用无角陶赛特羊作为主选父本,小尾寒羊和蒙古羊为主选母本的杂交组合是较为理想的选择,并为建立北方肉用绵羊新品种分子标记辅助选择技术体系打下坚实的基础。
The genetic diversity for Poll Dorset,Sall Tail Han sheep,Mongolian sheep and their hybrid offspring was studied using 12 microsatellite DNA markers(McM42、MAF65、OarFCB11、McM527、HSC、OarFCB20、OarAE101、RM0509、MNS0094、ILSTS004、BM1329、CSSM0004).The genetic heterrozygosity(He),polymorphism information content(PIC),dominate alleles frequency,Nei Standard Genetic Distance and cluster analysis were determined to evaluate the genetic vaviability within breeds(populations) and genetic relationship among breeds(populations).The results were as follows: The 12 microsatellite loci for 125 blood samples of the 5 populations were analyzed by vertical non-denatured popycrylamide gel electrophoresis(PAGE) figures.There was abundant popymorphism in 12 lozi,198 allels in total are detected,the mean of each locus was 16.5.
The polymorphism information content(PIC) of each site was abundant in 5 populations ,the minimum PIC was 0.7825;The genetic heterrozygosity(He) for Poll Dorset,Sall Tail Han sheep and Mongolian sheep was 0.8642,0.8558,0.8425 respectively;The maximal Nei Standard Distance(0.2327) among them was between Poll Dorset and Sall Tail Han sheep,the follows in order were respectly Poll Dorset and Mongolian sheep, Sall Tail Han sheep and Mongolian sheep.The microsatellite loci analysis showed that Poll Dorset and Small Tail sheep had the furthest relationship.The relationship was the closest between Small Tail sheep and Mongolian sheep.
The perfect project for New Mutton Sheep Breed in the north of China at Microsatellite DNA molecular level is that using Poll Dorset as male parent and Small
Tail Han sheep and Mongolian sheep as female parent.
对5个群体125只个体血液DNA样品的PCR产物进行了非变性聚丙烯酰胺凝胶电泳以及电泳图谱分析,结果共检测到198个等位基因,平均每个位点有16.5个等位基因,说明12个微卫星位点都具有较高的多态性。
通过计算等位基因频率、遗传杂合度、多态信息含量、Nei氏遗传距离和系统聚类分析,5个绵羊群体在不同位点的多态信息含量(PIC)普遍较高,基本上都在0.7以上;无角陶赛特、小尾寒羊和蒙古羊的平均杂合度分别为0.8642、0.8558、0.8425,两个杂交后代陶寒一代羊和陶蒙一代羊的平均杂合度分别为0.7900、0.7811;Nei氏标准遗传距离由远到近依次为无角陶赛特和小尾寒羊(0.2327)、无角陶赛特和蒙古羊(0.1859)、蒙古羊和小尾寒羊(0.1492),引进品种无角陶赛特和两个地方品种间的遗传距离绝对值都较大;结合非加权组对算术平均法(UPGMA)和邻近结合法(NJ)两种系统发育树的分析,无角陶赛特和小尾寒羊的亲缘关系最远,小尾寒羊和蒙古羊的亲缘关系较近。
从微卫星DNA分子水平进一步说明在北方肉用绵羊新品种培育中,用无角陶赛特羊作为主选父本,小尾寒羊和蒙古羊为主选母本的杂交组合是较为理想的选择,并为建立北方肉用绵羊新品种分子标记辅助选择技术体系打下坚实的基础。
The genetic diversity for Poll Dorset,Sall Tail Han sheep,Mongolian sheep and their hybrid offspring was studied using 12 microsatellite DNA markers(McM42、MAF65、OarFCB11、McM527、HSC、OarFCB20、OarAE101、RM0509、MNS0094、ILSTS004、BM1329、CSSM0004).The genetic heterrozygosity(He),polymorphism information content(PIC),dominate alleles frequency,Nei Standard Genetic Distance and cluster analysis were determined to evaluate the genetic vaviability within breeds(populations) and genetic relationship among breeds(populations).The results were as follows: The 12 microsatellite loci for 125 blood samples of the 5 populations were analyzed by vertical non-denatured popycrylamide gel electrophoresis(PAGE) figures.There was abundant popymorphism in 12 lozi,198 allels in total are detected,the mean of each locus was 16.5.
The polymorphism information content(PIC) of each site was abundant in 5 populations ,the minimum PIC was 0.7825;The genetic heterrozygosity(He) for Poll Dorset,Sall Tail Han sheep and Mongolian sheep was 0.8642,0.8558,0.8425 respectively;The maximal Nei Standard Distance(0.2327) among them was between Poll Dorset and Sall Tail Han sheep,the follows in order were respectly Poll Dorset and Mongolian sheep, Sall Tail Han sheep and Mongolian sheep.The microsatellite loci analysis showed that Poll Dorset and Small Tail sheep had the furthest relationship.The relationship was the closest between Small Tail sheep and Mongolian sheep.
The perfect project for New Mutton Sheep Breed in the north of China at Microsatellite DNA molecular level is that using Poll Dorset as male parent and Small
Tail Han sheep and Mongolian sheep as female parent.
引文
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