梨树休眠期贮藏营养的研究
详细信息    本馆镜像全文|  推荐本文 |  |   获取CNKI官网全文
摘要
本论文以鸭梨、黄金梨、雪花梨和八月红梨为试材,对鸭梨休眠期短枝及外围发育枝贮藏营养含量的变化和这四种梨贮藏营养在年生枝类中的含量分布状况进行了研究,以期为梨果优质丰产栽培提供理论依据。主要研究结果如下:
     不同树势鸭梨贮藏营养的变化都呈现相同的规律。外围发育枝和短枝贮藏营养的变化规律致。
     可溶性糖含量的变化呈现先升后降再升的变化趋势,枝木于1月底达到最高值,枝皮、花芽、叶芽都于1月中旬达到最高值:淀粉含量的变化与可溶性糖基本相反,呈现先降后升的变化趋势,枝木于1月底达到最低值,枝皮、花芽、叶芽都于1月中旬达到最低值。
     芽体蛋白质变化除萌芽前升高外,和枝木、枝皮的致,呈现降升降升的变化趋势,并于1月中旬升到最高值;芽体氨基酸变化与枝木、枝皮中的蛋白质正好相反,呈现先升后降再升的变化趋势,并于1月底达到最高值;枝木、枝皮中氨基酸变化致,先降后升再降,并于1月中旬降到最低值。
     枝木中磷含量的变化基本都呈下降趋势,枝皮中磷含量于休眠期变化不大,但各枝类都于1月中旬达到最大值,芽中磷的含量变化相对较剧烈,呈现降升降升的趋势。
     芽中钾的变化趋势同枝木中的致,先升后降,枝皮钾含量变化几乎和枝木的钾呈现互为消长的关系,先降后升,芽中钾最高值出现在1月底,枝木钾最高值出现1月中旬,枝皮钾于1月中旬达最低值。芽体、枝木和枝皮的变化致,都表现为先升后降的趋势。
     酚类物质的含量除叶芽于1月底达到最高值外,都于1月中旬达到最高值。壮树贮藏营养含量几乎都显著大于弱树。
     贮藏营养含量在年生枝中的分布基本都呈现短果枝>中果枝>长果枝,短发育枝>中发育枝>长发育枝,短果枝>短发育枝,中果枝>中发育枝,长果枝>长发育枝的规律。枝皮是可溶性糖、蛋白质、钾、酚类物质的主要贮藏部位;枝木是淀粉、主要的贮藏部位,也是磷休眠初期的主要贮藏部位;枝皮和枝木都是氨基酸的主要贮藏部位。淀粉是主要的碳素贮藏营养形态,蛋白质是主要的氮素贮藏营养形态。
This paper took Yali pear,Whangkeumbae pear,Xuehuali pear,and Bayuehong pear as experimental matrial. The changes of storage nourishment of tree and the distribution condition in annual shoot were studied during the period of dormancy , in order to supply the theory reference for cultivation of good quality and high yield. The main results were as follows:The changes of storage nourishment of Yali pear of different patterns all showed the same certain law. The variation law of storage nourishment of off-lying developmental branch and short branch were the same.The contents of soluble sugar first raised, later dropped and then raised. The soluble sugar of stem xylem reached the top contents in the last of January,while the top contents of stem phloem, blossom bud and growth bud all in the middle of January. The contents of starch first dropped and then raised. The trendency showed inverse relationship with that of the soluble sugar. The minimum content of stem xylem appeared in the last of January, while the minimum contents of stem phloem, blossom bud and growth bud all appeared in the middle of January.The changes tendency of protein contents of bud were inconcordance with that of stem xylem and stem phloem except ascending before germination, showed the tendency of decreased first and ascend later, the maximum contents were in the middle of January, the minimum contents were in the last of January. The changes tendency of amino acid contents of bud showed inverse relationship with that of the stem xylem and stem phloem, showed the tendency ascend first decreased later and ascend last, the maximum contents were in the last of January. The amino acid contents showed the same tendency of stem xylem and stem phloem, decreased first and asend later, the minimum contents were in the middle of January.The changes of P contents of stem xylem decreased during the period of dormancy, the tendency of that of P almost not changed, but the maximum contents of different types of shoots were in the middle of January. The changes of P contents of bud showed drastic relatively. It ascend, decreased and ascend, decreased again.The changes of K contents of bud were in the same of that of the stem xylem ,showed
    the tendency of ascend first and decreased later, the changes of K contents of stem phloem and stem xylem conversed. The maximum content of bud was in the last of January, the maximum content of stem xylem was in the middle of January, while the minimum content of stem xylem was in the middle of January.The changes of phenols of buds, stem phloem and stem xylem were the same, first raised then dropped , and growth bud reached the top contents in the last of January, blossom bud,stem phloem and stem xylem reached the top contents in the middle of January. The storage nourishment contents of vigorous tree was greater than weak tree, these can supply firm foundation for hibernation and growth, development in the next year.The storage nourishment contents of the short fruit shoots were higher than the middle fruit shoots, the middle fruit shoots were higher than the long fruit shoots, the short developmental branch was higher than the middle developmental branch, the middle developmental branch was higher than the long developmental branch, the short fruit shoots were higher than the short developmental branch, the middle fruit shoots were higher than the middle developmental branch, the long fruit shoots were higher than the long developmental branch. The main storage position of soluble suger, protein, K and phenols was stem phloem. The stem phloem and stem xylem both are the main storage position of amino acid. The main storage position of starch was stem xylem. The main storage position of P in the early dormancy period was stem xylem too. The main storage shape of carbon nutrition was starch, The main storage shape of nitrogen nutrition was protein.
引文
[1] 顾曼如,张杼,束怀瑞.苹果氮素研究初报[J].园艺学报,1981,8(4):21~28.
    [2] 夏国海,罗新书.苹果幼树秋季~(14)C同化物贮藏及再利用[J].园艺学报,1990,17(2):96~101.
    [3] 束怀瑞,黄镇,张连忠.苹果幼树碳素营养物质的贮藏及利用习性研究[J].山东农学院学报,1981(1):23~30.
    [4] 郝中宁,曾骧.秋季叶施尿素及乙烯利对枣树贮藏营养氮素的影响[J].园艺学报,1991,18(2):102~106.
    [5] 束怀瑞.果树栽培生理学[M].北京:农业出版社,1999,85,251.
    [6] 顾曼如,束怀瑞,周宏伟.苹果氮素营养研究Ⅳ.贮藏~(15)N的运转、分配特性[J].园艺学报,1986,13(1):25~30.
    [7] 王改萍.银杏营养贮藏蛋白质的变化规律研究[D].南京:南京林业大学,2003.
    [8] 顾曼如.苹果氮素营养研究:不同形态~(15)N的吸收运转特性[J].山东农业大学学报,1978,18(4):17~24.
    [9] Hill-cottingham DG. Chemical changes in apple tree issues following application of fertilizer nitrogen[J]. N. Agr. es., 1973, 8: 778~787.
    [10] 田维敏.树木营养贮藏蛋白质的细胞学、生物化学和生物学功能的研究[D].西安:西北大学,2002.
    [11] 束怀瑞.果树栽培生理学[M].北京:农业出版社,1999
    [12] Sanchez EE, Righetti TL, Sugar D, et al. Recycling of nitrogen in field-grown Cormice pears [J]. Hort. Sci, 1991, 66: 479~486.
    [13] Sanhez EE, Righetti TL, SugarD, et al. Effects of timing of nitrogen application on nitrogen partitioning between vegetative, reproductive, and structural components of mature Comice pears[J]. Hort. Sci, 1992, 67: 51~58.
    [14] Titus J S, Kang S M. Nitrogen metabolism, translocation and recycling in apple trees[J]. Hort Rev, 1982, 4: 204~246.
    [15] Taylor B K, May L H. The nitrogen nutrition of peach tree. The storage and mobilization of nitrogen in young trees[J]. Aust J Biol Sci, 1967, 20: 289~411.
    [16] Tromp J. Nutrient reserves of roots of fruit trees in particular carbohydration and nitrogen[J]. Plant and Soil, 1983, 71: 401~413.
    [17] Wetzel S, Demmers C, Greewood J S. Seasonally fluctuating barkproteins are a potential form of nitrogen storage in three temperature hard wood[J]. Planta, 1989, 178: 275~281.
    [18] 赵登超,姜远茂,彭福田,等.冬枣果实硬核期对~(15)N-尿素吸收、分配及再利用特性研究[J].应用生态学报,2006,17(1):27~30.
    [19] 张进,姜远茂,束怀瑞,等.不同施肥期沾化冬枣对~(15)N的吸收、分配及利用特性[J].园艺学报,2005,32(2):288~291.
    [20] Coleman G D, Chen T H, Fuchingami L H. Complementary DNA cloning of Popular bark storage protein control of its expression by photoperiod[J]. Plant Physial, 1992, 98: 687~693.
    [21] Feigenbaumn S, Bielorai H, Emer Y, et al. The fate of N labeled nitrogen supplied to mature citrus trees[J]. Plant and soil, 1987, 97: 178~187.
    [22] 王跃进,扈惠灵,王西平.晚秋叶施高浓度肥料对梨树翌年生长发育的效应[J].西北农林科技大学学报(自然科学版),2002,30(2):107~110.
    [23] 束怀瑞.果树栽培生理学[M].农业出版社,1999,234.
    [24] Jordi W, Stoopen G M Argiroudi I, et al. Accumulation of a 50KDa protein during leaf senescence of alstroemeria cut flowering stems[J]. Physologia piantarum, 1996, 98(4): 819~823.
    [25] AroraRL, Wisniewski M, Rowland LJ. Cold acclimation and alterations in dehydrin-like and bark storage proteins in the leaves of sibling deciduous and evergreen peach[J]. Journal of the American Society for HorticulturalScience, 1996, 121(5): 915~919.
    [26] 扈惠灵,王跃进,王西平.晚秋叶施高浓度肥料对梨树矿质营养状况的影响[J].西北农业学报,2002,11(2):63~66.
    [27] 贺香云.苹果树缺磷诊断方法及施磷技术[J].山西果树,2003,3:52.
    [28] 仝月澳,周厚基著.果树营养诊断法[M].北京.农业出版社,1982.
    [29] 王春梅,韩振海,李晓林,等.磷与VA菌根真菌对小金海棠苹果苗生长及营养的影响[J].园艺学报,2001,28(1):1~6.
    [30] 孙克刚译.世界钾肥供需形势和展望[J].农资科技,2000,(4):28~29.
    [31] 黄显淦,王勤,赵天才.钾素在我国果树优质增产中的作用[J].果树科学,2000,17(4):309~313.
    [32] 曲桂敏,束怀瑞,王鸿霞.钾对苹果树水分利用效率及有关参数的影响[J].土壤学报,2000,37(2):287~262.
    [33] 李宝江,林桂荣,刘凤君.矿质营养含量与苹果风味品质及耐贮性的关系[J].果树科学,1995,12(3):141~145.
    [34] 周伯瑜,黄文.苹果缺锌缺硼缺铁症及其防范[J].绿化与生活,1994,(3):23.
    [35] 王衍安,范伟国,张方爱,等.缺锌对苹果树生长和生理指标的影响[J].落叶果树,1999,(2):7~8.
    [36] 申建波,张富锁,毛达如.植物矿质营养的生态意义[J].生态农业研究,1997,5(1):22~26.
    [37] 于坤令,姜中武.浅谈苹果缺素症与平衡施肥问题[J].烟台果树,1998,2:13~15.
    [38] 张连忠,接玉玲,杨兴洪,等.落叶前根外追肥对苹果休眠期营养状况的影响[J].山东农业大学学报,1998,29(4):443~447.
    [39] Lockard RG. Phenolic compounds in two size controlling apple root- stacks[J]. J Amer Soc Hort Sci, 1982, 107(2): 183~186.
    [40] 胡仕碧,赵强,何首林.巨峰葡萄对钾素的吸收,分配,贮藏和再分配[J].园艺学报,1998,25(1):6~10
    [41] 顾曼如,彭福田,黄化成,等.苹果不同施10B量与施10B期对硼吸收和运转习性的研究[J].中国农业科学,1996,29(3):13~19
    [42] 顾曼如,彭福田,姜远茂,等.秋施硼肥在苹果植株中的分布与利用状态[J].园艺学报,1998,25(2):147~153
    [43] 扈惠灵,王跃进,王西平.晚秋叶施高浓度肥料对梨树矿质营养状况的影响[J].西北农业学报,2002,11(2):63~66
    [44] 韩其谦,邢文通,马荣旺,等.杏树年周年淀粉消长规律的观察[J].河北农业大学报,1988,11(1):1~10.
    [45] 杨秀苹.油桃花芽自然休眠期间呼吸机制的研究[D].安徽:安徽农业大学,2004.
    [46] 戴文浩.果梅若干生理指标的研究[J].果树科学,1997,14(4):248~251.
    [47] Siminovitch D. Evidence from increase in ribonucleic acid and protein synthesis in autumn for increase in protoplasm during the frost-hardening of block locust bark cells[J]. Can J Bot, 1963, 41: 1301~1308.
    [48] Pomeroy M K, Siminovitch D, wightman F. Seasonal biochemical changes in the living bark and needles of red pine(Pinus resinosa) in relation to adaptation to freezing[J]. Can J Bot, 1970, 48: 953~967.
    [49] 高素华,郭建平,张国民,等.低温对玉米幼苗生理反应的影响[J].应用气象学报,1999,10(2):238~242.
    [50] Guy. G. L.. Niemi. K. J. and Brambl, R, 1985, Altered gene expression during cold acclimation of spinach[J]. Proc. Natl. Acad. Sci., 82: 3673~3677.
    [51] 杨洪强,接玉玲,黄天栋,等.苹果幼树根系越冬期氮代谢及覆膜效应研究[J].园艺学报,1996,23(4):329~333.
    [52] 李霞.呼吸代谢对甜樱桃自然休眠的调控及破眠技术研究[D].山东:山东农业大学,2004.
    [53] 董文成.苹果幼树喷钾防止冻害和抽条试验[J].河北果树,1990,1:22~25.
    [54] 西北农业大学主编.基础生物化学实验指导[M].陕西:陕西科技出版社,1986,20~27.
    [55] 山东农学院编.植物生理学实验指导[M].山东:山东科技出版社,1985:196~205,239~245.
    [56] 南京农学院主编.土壤农化分析[M].北京:农业出版社,1980:193~197.
    [57] 晏本菊,李焕秀.梨外植体体褐变与多酚氧化酶及酚类物质的关系[J].四川农业大学学报,1998,16(3):310~313.
    [58] 杨洪强,接玉玲,等.苹果幼树根系越冬期氮代谢及覆膜效应研究[J].园艺学报,1996,23(4):329~334.
    [59] 孙继河,秋季如何提高果树贮藏营养水平[J].农业科技与信息,1997,11:19.
    [60] 王中营,果树学概论[M].北京:农业出版社,1994,24.
    [61] 束怀瑞.果树栽培生理学[M].北京:农业出版社,1993:288-290,52.
    [62] 冷平,张国军,吴晓云,等.秋冬季节柿属植物树体内酚类物质含量的变化[J].中国农业大学学报,2001,6(1):63~67.
    [63] 郭先锋,张玉龙,刘和.丹霞苹果树体器官中酚类物质含量的年变化[J].果树学报,2004,21(6):606~608.
    [64] 王勇,吴国良,李登科,等.核桃树体内酚类物质含量的变化[J].果树学报,2003,20(4):325~327.
    [65] 扈惠灵.梨树晚秋叶施高浓度肥料的效应研究[D].杨陵:西北农林科技大学,2000.
    [66] 胡仕碧,赵强,何首林.巨峰葡萄对钾素的吸收、分配、贮藏和再分配[J].园艺学报,1998, 25(1):6~10.
    [67] 张进,姜远茂,赵登超,等.沾化冬枣萌芽前对枝条尿素的吸收、分配及再利用特性[J].植物营养与肥料学报,2004,10(6):671~673.
    [68] 管长志,曾骧,孟昭清.巨峰葡萄晚秋叶施~(15)N-尿素的吸收、运转、贮藏和再分配[J].园艺学报,1993,20(3):237~242.
    [69] 顾曼如,束怀瑞,周宏伟.苹果氮素营养研究Ⅲ.根外追~(15)N及其吸收、运转特性[J].园艺学报,1985,12(2):89~94.
    [70] 韩振海,曾骧,王福钧.晚秋叶施尿素和生长调节剂对富士苹果幼树贮藏氮素的影响[J].园艺学报,1992,19(1):15~21.
    [71] 徐季娥,林裕益,吕瑞江,等.鸭梨秋施~(15)N-尿素的吸收与分配[J].园艺学报,1993,20(2):145~149.
    [72] 张进,姜远茂,赵登超,等.沾化冬枣对春施氮肥的吸收、分配及利用特性[J].植物营养与肥料学报 2005,11(3):420~423.
    [73] 闫兴富.大叶桃花心木幼苗营养贮藏蛋白质的研究[D].海南:华南热带农业大学,2003.
    [74] 潘瑞英.荔枝营养贮藏蛋白质的分离鉴定及其与开花结实的关系[D].海南:华南热带农业大学,2004.
    [75] 郭彦青.杨树营养贮藏蛋白质动态变化规律的研究[D].南京:南京林业大学,2005.

© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号

地址:北京市海淀区学院路29号 邮编:100083

电话:办公室:(+86 10)66554848;文献借阅、咨询服务、科技查新:66554700