低能离子辐射对二倍体和同源四倍体水稻的细胞学效应研究
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摘要
本项研究以二倍体和四倍体水稻为试验材料。对离子注入处理后所引起M1代及M2代水稻根尖细胞内染色体的畸变类型、畸变率,有丝分裂指数等细胞学特征及其对发芽率的影响进行了系统研究。另外,初步探究了水稻根尖细胞在分化过程中微丝网络的分布格局的变化。本实验的研究结果重要包括如下几个方面：
1.低能氮离子注入引起M1代水稻品种的根尖细胞在有丝分裂中出现了不同类型的染色体畸变,如染色体桥,染色体小断片,落后染色体等。根尖细胞染色体的畸变率与氮离子的处理剂量之间并非呈现明显的变化趋势,而是呈现一定的随机性。低能氮离子注入对某些水稻品种根尖细胞的有丝分裂具有促进作用,M1代根尖细胞有丝分裂指数升高。某些注入剂量对M1代水稻品系根尖细胞的有丝分裂具有促进作用,而某些剂量却抑制了根尖细胞的有丝分裂。
2.低能氮离子注入对M2代水稻品种的根尖细胞的有丝分裂也具有促进作用,还可以引起M2代根尖细胞的染色体的畸变,其主要的畸变类型有染色体桥,染色体小断片,落后染色体等。这与M1代根尖细胞染色体的畸变类型相似,说明经离子注入处理后的染色体畸变具有一定的遗传性。但染色体在传代过程中也具有一定的修复作用,表现在M2代根尖细胞染色体的畸变率明显低于M1代。
3.经低能氮离子处理后的水稻干种子种植于试验田中观察其减数分裂的情况发现在M1代水稻的花粉母细胞中的染色体出现了染色体桥,染色体小断片,落后染色体等类似于有丝分裂的畸变类型。并且还可见游离的染色体和染色体提前分离等特殊现象。
4.初步研究了水稻根尖细胞分化过程中微丝分布格局的变化。发现水稻根尖细胞分化过程中从分生区至成熟区细胞内的微丝分布格局确实发生了改变。分生区细胞根据细胞所在的分裂时期主要有两种状态的微丝分布。间期细胞中无明显的微丝网络,整个细胞被激发出均匀、明亮的绿色荧光,细胞核清晰可见。分裂期的细胞细胞核区周围有大量的明亮的荧光呈“笼”状分布,细胞质中可见荧光较细胞核周围弱,并且微丝成束的出现,微丝束较多,在细胞膜周缘荧光较亮,表明细胞膜周缘中微丝分布密集。伸长区细胞,细胞核周缘有明显的微丝束向四处呈现辐射状分布,从不同层面的扫描结果可见在核周围多个层次上看到一些纤索的明亮的绿色荧光。成熟区细胞,细胞核周围有较强的荧光,而细胞质中几乎见不到荧光。
The damage of the root tip cell chromosomes, including the rate of distortion, the type of aberration and the mitotic index etc. of the Ml and M2 generations coused by low energy ion implantation was studied in this paper, using diploid and tetraploid rice as materials. In addition, the distribution pattern of microfilaments network of the rice root tip cells during differentiation are preliminary researched. The results of this study was as follows.
1. Implantation of low energy N+ ion beam can cause different types of chromosomal aberrations of the M1 root tip cells in mitosis, such as chromosome bridge, chromosome small fragments, laggards, etc. Chromosome aberration rate in root-tip cells is not proportional to N+ ion treatment dosages. but showes some randomness. Low energy N+ ion beam Implantation on some rice varieties mitotic of root tip cells could promote the mitotic index of Ml generation root tip cells increased. Some injected dose can promote mitosis of M1 generation rice strains root-tip cells, and some doses inhibited mitosis of root tip cells.
2. Low energy N+ion beam implantation on M2 generation rice varieties mitotic of root tip cells also could promote, and lead to M2 generation root tip cells of the chromosome aberration, the main types of distortion, chromosome bridge, chromosome small fragments, laggards, etc. M2 with Ml generation root tip cells of chromosome aberration of a similar type. M2 generation of root tip cell chromosome aberration rate lower than the M1.
3. Seeds of rice treated by low energy N+ion were planted at rice experimental field of Institute of Rice Science, Xinxiang Agricutural Technology Research Academy. The meiosis of pollen mother cells in M1 generation were observed. The result showed that the chromosomal aberrations were similar to the type of mitotic aberration, appearing chromosome bridge, small chromosomal fragments, lagging chromosomes. Besides that, the free chromosomes, chromosome separation and other special phenomenon ahead of schedule were found.
4. The distribution pattern of microfilaments network of the rice root tip cells during differentiation are preliminary researched. It was found that the actin distribution pattern in rice root tip cells during differentiation from meristematic zone to the mature region were significant different. There were two states of actin distribution in the meristem region. In interphase cells, There were no obvious microfilament network, the cell was inspired by the uniform, bright green fluorescence, and the nucleus is clearly visible. In mitotic phase cells, nucleus were surrounded by a large number of bright fluorescence, Showed a "cage"-like distribution. There were a large number of fluorescent nuclei peripheral radial distribution of the four presented in elongation region cells. The nucleus were surrounded by bright fluorescence, while almost no fluorescence in cytoplasm in mature zone cells.
1.低能氮离子注入引起M1代水稻品种的根尖细胞在有丝分裂中出现了不同类型的染色体畸变,如染色体桥,染色体小断片,落后染色体等。根尖细胞染色体的畸变率与氮离子的处理剂量之间并非呈现明显的变化趋势,而是呈现一定的随机性。低能氮离子注入对某些水稻品种根尖细胞的有丝分裂具有促进作用,M1代根尖细胞有丝分裂指数升高。某些注入剂量对M1代水稻品系根尖细胞的有丝分裂具有促进作用,而某些剂量却抑制了根尖细胞的有丝分裂。
2.低能氮离子注入对M2代水稻品种的根尖细胞的有丝分裂也具有促进作用,还可以引起M2代根尖细胞的染色体的畸变,其主要的畸变类型有染色体桥,染色体小断片,落后染色体等。这与M1代根尖细胞染色体的畸变类型相似,说明经离子注入处理后的染色体畸变具有一定的遗传性。但染色体在传代过程中也具有一定的修复作用,表现在M2代根尖细胞染色体的畸变率明显低于M1代。
3.经低能氮离子处理后的水稻干种子种植于试验田中观察其减数分裂的情况发现在M1代水稻的花粉母细胞中的染色体出现了染色体桥,染色体小断片,落后染色体等类似于有丝分裂的畸变类型。并且还可见游离的染色体和染色体提前分离等特殊现象。
4.初步研究了水稻根尖细胞分化过程中微丝分布格局的变化。发现水稻根尖细胞分化过程中从分生区至成熟区细胞内的微丝分布格局确实发生了改变。分生区细胞根据细胞所在的分裂时期主要有两种状态的微丝分布。间期细胞中无明显的微丝网络,整个细胞被激发出均匀、明亮的绿色荧光,细胞核清晰可见。分裂期的细胞细胞核区周围有大量的明亮的荧光呈“笼”状分布,细胞质中可见荧光较细胞核周围弱,并且微丝成束的出现,微丝束较多,在细胞膜周缘荧光较亮,表明细胞膜周缘中微丝分布密集。伸长区细胞,细胞核周缘有明显的微丝束向四处呈现辐射状分布,从不同层面的扫描结果可见在核周围多个层次上看到一些纤索的明亮的绿色荧光。成熟区细胞,细胞核周围有较强的荧光,而细胞质中几乎见不到荧光。
The damage of the root tip cell chromosomes, including the rate of distortion, the type of aberration and the mitotic index etc. of the Ml and M2 generations coused by low energy ion implantation was studied in this paper, using diploid and tetraploid rice as materials. In addition, the distribution pattern of microfilaments network of the rice root tip cells during differentiation are preliminary researched. The results of this study was as follows.
1. Implantation of low energy N+ ion beam can cause different types of chromosomal aberrations of the M1 root tip cells in mitosis, such as chromosome bridge, chromosome small fragments, laggards, etc. Chromosome aberration rate in root-tip cells is not proportional to N+ ion treatment dosages. but showes some randomness. Low energy N+ ion beam Implantation on some rice varieties mitotic of root tip cells could promote the mitotic index of Ml generation root tip cells increased. Some injected dose can promote mitosis of M1 generation rice strains root-tip cells, and some doses inhibited mitosis of root tip cells.
2. Low energy N+ion beam implantation on M2 generation rice varieties mitotic of root tip cells also could promote, and lead to M2 generation root tip cells of the chromosome aberration, the main types of distortion, chromosome bridge, chromosome small fragments, laggards, etc. M2 with Ml generation root tip cells of chromosome aberration of a similar type. M2 generation of root tip cell chromosome aberration rate lower than the M1.
3. Seeds of rice treated by low energy N+ion were planted at rice experimental field of Institute of Rice Science, Xinxiang Agricutural Technology Research Academy. The meiosis of pollen mother cells in M1 generation were observed. The result showed that the chromosomal aberrations were similar to the type of mitotic aberration, appearing chromosome bridge, small chromosomal fragments, lagging chromosomes. Besides that, the free chromosomes, chromosome separation and other special phenomenon ahead of schedule were found.
4. The distribution pattern of microfilaments network of the rice root tip cells during differentiation are preliminary researched. It was found that the actin distribution pattern in rice root tip cells during differentiation from meristematic zone to the mature region were significant different. There were two states of actin distribution in the meristem region. In interphase cells, There were no obvious microfilament network, the cell was inspired by the uniform, bright green fluorescence, and the nucleus is clearly visible. In mitotic phase cells, nucleus were surrounded by a large number of bright fluorescence, Showed a "cage"-like distribution. There were a large number of fluorescent nuclei peripheral radial distribution of the four presented in elongation region cells. The nucleus were surrounded by bright fluorescence, while almost no fluorescence in cytoplasm in mature zone cells.
引文
[1]徐刚.日本离子束辐照设施的利用研究动态[J].核农学报1999,13(2)：118-119.
[2]于增亮.离子束生物技术引论[M].合肥：安徽科学技术出版社,1998.
[3]余增亮,霍裕平.离子注入生物学研究述评[J].安徽农业大学学报,1994,21(3)：221-225.
[4]吴兰佩,崔海瑞,包忠谋等.氮离子束等诱变机理研究(二)[J].安徽农业大学学报,1994,21(3)：233-240.
[5]任海云,黄忠连,陈志玲等.低能氮离子注入对花粉萌发及微丝管架的影响[J).科学通报,2000,45(2)：390-394.
[6]顾月华,程颖红,唐健彬等.离子束注入对细胞有丝分裂微管骨架影响的研究[J]激光生物学报,1997,6(3)：1110-1113.
[7]宋道军,姚建铭,吴丽芳等.离子注入微生物产生“马鞍型”存活曲线的可能性作用机制.核技术2001(3)：129-132.
[8]贾瀚超,张斓,周云龙.低能离子注入技术在生物学中的研究和应用.生物学通报,2007,第42卷第2期.
[9]张根发,石小明,聂艳丽等.等能N+注入与γ辐射拟南芥可溶性蛋白和淀粉酶(AMY)、酯酶(EST)酶活和同工酶影响的比较分析.高技术通讯,2005,15(2)：84—91.
[10]宋道军,余汛,韩建伟等.离子注入不同辐射敏感性微生物自由基与存活关系的研究,激光生物学报,1998,7(4)：245—251.
[11]Zhang Genfa,Li Ke,Shi Xiaoming. Comparative Study on Effects of low Energy N+ Implantations and γ-ray Radiations on Heredity and Development of Arabidopsis Thaliana.Plasma Science & Technology,2005,7(3):2879—2885.
[12]杨建波,吴李军,吴家道等.离子束辐照M13mpl8 DNA结构和功能影响的初步研究,中国科学B辑,1995,25(12)：1273—1278.
[13]卞坡,谷运红,霍裕平等.离子束的生物效应及应用.河南农业大学学报,1999,33(2)：178—181.
[14]Shao Chunlin,Aoki Mizubo,Furusawa Yoshiya.Bystander effect on growth stimulatino in neoplastic HSGC cell induced by heavy-ion irradiation. Radiat Environ Biophys,2003,42:183—187.
[15]Wu Lijun,Tom K.Hei, G Randers—Pehrson, Yu Zengliang. Targeted microbeam irraditions:Study on the radiological effects and the accuracy of a particle irradiations.Journal of Radiations Research and Radiations Processing.2000,18(1):57—63.
[16]杨银,吴李君.定位辐射拟南芥种胚茎端分生组织有诱导的长程旁效应.第四次全国离子束生物工程大会论文汇编。2007,26—27.
[17]李国平.低能离子束注入松科花粉的细胞学效应,郑州大学博士学位论文,2007.
[18]黄群策.离子束介导技术在实际应用中所存在的问题及研究对策.郑州大学学报(理学 版).2007,39(2)：169—176.
[19]Yu Zengliang. The progress of ion beam bioengineering in China.Solid state phenomena,2005,107:25—30.
[20]Yu Zengliang, Shao Cl Dose effect of the tyrosine sample implanted by a low energy N+ions beam,Radiat phys Chem,1994,43(4):349—351.
[21]黄群策,李玉峰.离子束生物技术在水稻育种中的应用前景.杂交水稻.2002,17(5)：5—8.
[22]黄群策,梁秋霞,李玉峰等.低能氮离子注入同源四倍体水稻的生物学效应.激光生物学报.2003,12(5)：355—359.
[23]黄群策,代西梅.低能氮离子束对不同倍性水稻的诱变效应.杂交水稻.2004,19(3)：57—61.
[24]黄群策,李玉峰.离子束生物技术在水稻育种中的应用前景.杂交水稻(HYBRIDRICE).2002,17(5):5—8
[25]谢慧博.同源四倍体水稻的诱导及其胚乳蛋白差异表达结果的初步研究.郑州大学硕士学位论文.2007.
[26]宋文昌,张玉华.水稻四倍化及其对农艺性状和营养成分的影响.农作物学报,1992,18(2)：138—144.
[27]代西梅,黄群策,李国平等.同源四倍体水稻花粉发育特征.中国水稻科学,2006,20(2)：165-170.
[28]HUAN G Q un-ce. Polypoid rice and its potential value [J]. Hybrid Rice,2001,16(1): 1--3.
[29]黄群策,孙梅元,邓启云.多倍体水稻及其潜在价值.杂交水稻,2001,16(1)：1-3.
[30]裴新澍.多倍体诱导与育种.上海科学技术出版社.1963,31—39.
[31]黄慧君,黄道强.水稻体细胞同源四倍体的人工诱导及遗传特性研究.广东农业科学.1995.01：9—12.
[32]黄群策.多倍体水稻及其潜在的价值.杂交水稻.2001,01：1—3.
[33]谢慧波,黄群策,李国平等.不同倍性水稻胚乳蛋白的差异表达研究.遗传.2007,29(3)：360—364.
[34]黄群策.同源三倍体水稻的结实特性研究.湖南农业科学.1998,2(1)：14—15.
[35]林如,黄群策.同源四倍体水稻紫血稻(4)在受精前后的特异性研究.中国农学通报.1999,5：30—33.
[36]Huang Q unce, L I Yufeng, Prospect of ion beam Bio-technology in rice breeding [J]. Hybrid R ice,2002,17 (5):48--50.
[37]黄群策,梁秋霞,李玉峰等.低能氮离子注入同源四倍体水稻的生物学效应[J].激光生物学报.2003,12(5)：355—359.
[38]Parthasarathy, M.V.& Muhlethaler,K,1972,J. Ultrastruct. Res,38:46.
[39]LUIS V, SYLVESTER T McKenna, PETER K H. Actin polymerization is essential for pollen tube growth [J].Molecular Biology of the Cell,2001,12:2534-2545.
[40]LIY(李岩),YANLF(阎隆飞),XU SHX(徐是雄)Distribution of F-actin and microtubules in pollen and pollentube of Lilium davidii[J]. Acta Botanica Sinica(植物学报),1998,40(10)：890-894(inChinese).
[41]丛斌,许萍,杨茂成等.小麦根尖细胞分化过程中微丝骨架分布格局[J].复旦学报(自然科学版),1996,VOI。35 No 6：661—664.
[42]温贤芳.中国核农学(M).河南：河南科学技术出版社,1999,601-613.
[43]陈佳珥.中国核技术发展战略[M].北京：科学出版社,1991,140-156.
[44]李玉峰,黄群策,梁运章.氮离子注入对同源四倍体水稻IR36萌发的影响[J].核农学报,2006,20(5)：403～405.
[45]赵惠玲,王青.改良植物根尖压片法观察染色体[J].太原师范专科学校学报,2001,3：26-27.
[46]杨旭,代西梅.简易压片法观察水稻有丝分裂和减数分裂行为[J].安徽农业科学,2009,37(14)：6325-6327.
[47]胡延吉.植物育种学.北京：高等教育出版社,2003：198.
[48]周云龙.植物生物学[M].北京：高等教育出版社,1999,59-60.
[49]黄群策,梁秋霞,李玉峰.低能氮离子注入同源四倍体水稻的生物学效应[J].激光生物学报,2003,12(5)：355～359.
[50]曲颖,李文建,周立斌.重离子辐射植物的诱变效应研究及应用[J].原子核物理评论,2007,12(4)：294-298.
[51]徐明照,周立人,程备久.氮离子注入玉米种子的细胞生物学效应[J].中国农学通报,2007,23(11)：77—80.
[52]Rydberd,B Radiation-induced heat-labile sites that convert into DNA double-strand breaks.Radiat.Res.,2000,153,805-812.
[53]Hase Y, Yamaguchi M, lnoue M, et al. Int J Radiat Biol,2002,78(9):799.
[54]Wu LF, Yu ZI. Radiat Environ Biophys,2001,40:53.
[55]王学栋,吴跃进,余增亮.等.安徽农业科学[J].1988,3：95.
[56]李玉峰,黄群策,梁云章.氮离子注入对同源四倍体水稻IR36萌发的影响[J].核农学报,2006,20(5)：403-405.
[57]王敏琴,彭振英,蔡雁峰,等.一种获得分散良好的植物染色体压片技术[J].生物学通报,2007,42(4)：50-51.
[58]周云龙.植物生物学[M].北京：高等教育出版社,1999,59-60.
[59]高健.钻一60Gmama射线辐照中国水仙的诱变效应和机理研究[M].中国林业科学研究院博士毕业论文.北京.中国林业科学研究院.2000年7月.
[60]代西梅,黄群策,李国平,等.同源四倍体水稻花粉发育特征[J].中国水稻科学(Chinese J Rice Sci),2006,20(2):165-170.
[61]丛斌,许萍,等.小麦根尖细胞分化过程中微丝骨架分布格局[J].复旦学报1996,35(6)：661—664.
[2]于增亮.离子束生物技术引论[M].合肥：安徽科学技术出版社,1998.
[3]余增亮,霍裕平.离子注入生物学研究述评[J].安徽农业大学学报,1994,21(3)：221-225.
[4]吴兰佩,崔海瑞,包忠谋等.氮离子束等诱变机理研究(二)[J].安徽农业大学学报,1994,21(3)：233-240.
[5]任海云,黄忠连,陈志玲等.低能氮离子注入对花粉萌发及微丝管架的影响[J).科学通报,2000,45(2)：390-394.
[6]顾月华,程颖红,唐健彬等.离子束注入对细胞有丝分裂微管骨架影响的研究[J]激光生物学报,1997,6(3)：1110-1113.
[7]宋道军,姚建铭,吴丽芳等.离子注入微生物产生“马鞍型”存活曲线的可能性作用机制.核技术2001(3)：129-132.
[8]贾瀚超,张斓,周云龙.低能离子注入技术在生物学中的研究和应用.生物学通报,2007,第42卷第2期.
[9]张根发,石小明,聂艳丽等.等能N+注入与γ辐射拟南芥可溶性蛋白和淀粉酶(AMY)、酯酶(EST)酶活和同工酶影响的比较分析.高技术通讯,2005,15(2)：84—91.
[10]宋道军,余汛,韩建伟等.离子注入不同辐射敏感性微生物自由基与存活关系的研究,激光生物学报,1998,7(4)：245—251.
[11]Zhang Genfa,Li Ke,Shi Xiaoming. Comparative Study on Effects of low Energy N+ Implantations and γ-ray Radiations on Heredity and Development of Arabidopsis Thaliana.Plasma Science & Technology,2005,7(3):2879—2885.
[12]杨建波,吴李军,吴家道等.离子束辐照M13mpl8 DNA结构和功能影响的初步研究,中国科学B辑,1995,25(12)：1273—1278.
[13]卞坡,谷运红,霍裕平等.离子束的生物效应及应用.河南农业大学学报,1999,33(2)：178—181.
[14]Shao Chunlin,Aoki Mizubo,Furusawa Yoshiya.Bystander effect on growth stimulatino in neoplastic HSGC cell induced by heavy-ion irradiation. Radiat Environ Biophys,2003,42:183—187.
[15]Wu Lijun,Tom K.Hei, G Randers—Pehrson, Yu Zengliang. Targeted microbeam irraditions:Study on the radiological effects and the accuracy of a particle irradiations.Journal of Radiations Research and Radiations Processing.2000,18(1):57—63.
[16]杨银,吴李君.定位辐射拟南芥种胚茎端分生组织有诱导的长程旁效应.第四次全国离子束生物工程大会论文汇编。2007,26—27.
[17]李国平.低能离子束注入松科花粉的细胞学效应,郑州大学博士学位论文,2007.
[18]黄群策.离子束介导技术在实际应用中所存在的问题及研究对策.郑州大学学报(理学 版).2007,39(2)：169—176.
[19]Yu Zengliang. The progress of ion beam bioengineering in China.Solid state phenomena,2005,107:25—30.
[20]Yu Zengliang, Shao Cl Dose effect of the tyrosine sample implanted by a low energy N+ions beam,Radiat phys Chem,1994,43(4):349—351.
[21]黄群策,李玉峰.离子束生物技术在水稻育种中的应用前景.杂交水稻.2002,17(5)：5—8.
[22]黄群策,梁秋霞,李玉峰等.低能氮离子注入同源四倍体水稻的生物学效应.激光生物学报.2003,12(5)：355—359.
[23]黄群策,代西梅.低能氮离子束对不同倍性水稻的诱变效应.杂交水稻.2004,19(3)：57—61.
[24]黄群策,李玉峰.离子束生物技术在水稻育种中的应用前景.杂交水稻(HYBRIDRICE).2002,17(5):5—8
[25]谢慧博.同源四倍体水稻的诱导及其胚乳蛋白差异表达结果的初步研究.郑州大学硕士学位论文.2007.
[26]宋文昌,张玉华.水稻四倍化及其对农艺性状和营养成分的影响.农作物学报,1992,18(2)：138—144.
[27]代西梅,黄群策,李国平等.同源四倍体水稻花粉发育特征.中国水稻科学,2006,20(2)：165-170.
[28]HUAN G Q un-ce. Polypoid rice and its potential value [J]. Hybrid Rice,2001,16(1): 1--3.
[29]黄群策,孙梅元,邓启云.多倍体水稻及其潜在价值.杂交水稻,2001,16(1)：1-3.
[30]裴新澍.多倍体诱导与育种.上海科学技术出版社.1963,31—39.
[31]黄慧君,黄道强.水稻体细胞同源四倍体的人工诱导及遗传特性研究.广东农业科学.1995.01：9—12.
[32]黄群策.多倍体水稻及其潜在的价值.杂交水稻.2001,01：1—3.
[33]谢慧波,黄群策,李国平等.不同倍性水稻胚乳蛋白的差异表达研究.遗传.2007,29(3)：360—364.
[34]黄群策.同源三倍体水稻的结实特性研究.湖南农业科学.1998,2(1)：14—15.
[35]林如,黄群策.同源四倍体水稻紫血稻(4)在受精前后的特异性研究.中国农学通报.1999,5：30—33.
[36]Huang Q unce, L I Yufeng, Prospect of ion beam Bio-technology in rice breeding [J]. Hybrid R ice,2002,17 (5):48--50.
[37]黄群策,梁秋霞,李玉峰等.低能氮离子注入同源四倍体水稻的生物学效应[J].激光生物学报.2003,12(5)：355—359.
[38]Parthasarathy, M.V.& Muhlethaler,K,1972,J. Ultrastruct. Res,38:46.
[39]LUIS V, SYLVESTER T McKenna, PETER K H. Actin polymerization is essential for pollen tube growth [J].Molecular Biology of the Cell,2001,12:2534-2545.
[40]LIY(李岩),YANLF(阎隆飞),XU SHX(徐是雄)Distribution of F-actin and microtubules in pollen and pollentube of Lilium davidii[J]. Acta Botanica Sinica(植物学报),1998,40(10)：890-894(inChinese).
[41]丛斌,许萍,杨茂成等.小麦根尖细胞分化过程中微丝骨架分布格局[J].复旦学报(自然科学版),1996,VOI。35 No 6：661—664.
[42]温贤芳.中国核农学(M).河南：河南科学技术出版社,1999,601-613.
[43]陈佳珥.中国核技术发展战略[M].北京：科学出版社,1991,140-156.
[44]李玉峰,黄群策,梁运章.氮离子注入对同源四倍体水稻IR36萌发的影响[J].核农学报,2006,20(5)：403～405.
[45]赵惠玲,王青.改良植物根尖压片法观察染色体[J].太原师范专科学校学报,2001,3：26-27.
[46]杨旭,代西梅.简易压片法观察水稻有丝分裂和减数分裂行为[J].安徽农业科学,2009,37(14)：6325-6327.
[47]胡延吉.植物育种学.北京：高等教育出版社,2003：198.
[48]周云龙.植物生物学[M].北京：高等教育出版社,1999,59-60.
[49]黄群策,梁秋霞,李玉峰.低能氮离子注入同源四倍体水稻的生物学效应[J].激光生物学报,2003,12(5)：355～359.
[50]曲颖,李文建,周立斌.重离子辐射植物的诱变效应研究及应用[J].原子核物理评论,2007,12(4)：294-298.
[51]徐明照,周立人,程备久.氮离子注入玉米种子的细胞生物学效应[J].中国农学通报,2007,23(11)：77—80.
[52]Rydberd,B Radiation-induced heat-labile sites that convert into DNA double-strand breaks.Radiat.Res.,2000,153,805-812.
[53]Hase Y, Yamaguchi M, lnoue M, et al. Int J Radiat Biol,2002,78(9):799.
[54]Wu LF, Yu ZI. Radiat Environ Biophys,2001,40:53.
[55]王学栋,吴跃进,余增亮.等.安徽农业科学[J].1988,3：95.
[56]李玉峰,黄群策,梁云章.氮离子注入对同源四倍体水稻IR36萌发的影响[J].核农学报,2006,20(5)：403-405.
[57]王敏琴,彭振英,蔡雁峰,等.一种获得分散良好的植物染色体压片技术[J].生物学通报,2007,42(4)：50-51.
[58]周云龙.植物生物学[M].北京：高等教育出版社,1999,59-60.
[59]高健.钻一60Gmama射线辐照中国水仙的诱变效应和机理研究[M].中国林业科学研究院博士毕业论文.北京.中国林业科学研究院.2000年7月.
[60]代西梅,黄群策,李国平,等.同源四倍体水稻花粉发育特征[J].中国水稻科学(Chinese J Rice Sci),2006,20(2):165-170.
[61]丛斌,许萍,等.小麦根尖细胞分化过程中微丝骨架分布格局[J].复旦学报1996,35(6)：661—664.