茵陈蒿和桑叶的成分以及固相色谱法对茵陈蒿汤效应物质的研究
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摘要
通过各种现代色谱技术(大孔树脂柱色谱、硅胶柱色谱、Sephadex LH-20凝胶柱色谱、反相ODS中低压柱色谱和HPLC)从茵陈蒿的水提物中,分离得到33个单体化合物,通过理化性质和光谱分析,鉴定了其中30个化合物的结构,其中新化合物2个,3个聚炔类为该属植物中首次分离得到,分别为:6'-O-caffeoyl-p-hydroxy-acetophenone-4-O-β-D-glucopyranoside(1);9-[1-(3,4-dihydroxyphenyl)ethyl]benzene-adenin e(2);3(R)-癸-4,6,8-三炔-1,3-二醇(3);3(R)-癸烯-4,6-二炔-1,3,8-三醇(4);3(R)-9-癸-4,6,8-三炔-1,3-二醇一1-O-β-D-吡喃葡萄糖苷(5);7-羟基-香豆素(6);6,7-二甲氧基-香豆素(7);6-甲氧基-7-羟基-香豆素(8);7-甲氧基-香豆素(9);对羟基苯乙酮(10);4-羟基-3-甲氧基苯乙酮(11);烟酸(12);苯甲醇-O-β-D-吡喃葡萄糖苷(13);苯甲酸(14);乙二酸(15);丁二酸(16);槲皮素(17):金丝桃苷(18);胸腺嘧啶(19);胸腺嘧啶脱氧核苷(20);尿嘧啶(21);香草醛(22);香草酸(23);对羟基苯乙酮-4-O-β-D-吡喃葡萄糖苷(24);邻羟基苯甲酸(25);3-甲氧基咖啡酸-4-O-β-D-吡喃葡萄糖苷(26);槲皮素-7-α-D-吡喃鼠李糖苷(27);正丁基-β-D-吡喃果糖苷(28);loliolide(29);邻苯二甲酸二(2-乙基-己基)酯(30)。
同时从桑叶的水提物中,分离得到31个单体化合物,通过理化性质和光谱分析,鉴定了其中25个化合物的结构,其中分别为acetyl-loliolide(1);对羟基苯甲酸(2);dehydrololiolide(3);loliolide(4);邻苯二酚(5);莨菪亭(6);苯甲醇-O-β-D-吡喃葡萄糖苷(7):3β-hydroxy-5α,6α-epoxy-7-megastimen-9-one(8);6,7-二羟基香豆素(9);环(亮氨酸-异亮氨酸)(10);槲皮素(11);3,4-二羟基苯甲酸(12);异落叶松脂素(13);刺五加酮(14);7,8-二羟基香豆素(15);环(丙氨酸-亮氨酸)(16):香草酸-4-O-β-D-葡萄糖苷(17);胸腺嘧啶(18);胸腺嘧啶脱氧核苷(19);环(苯丙氨酸-酪氨酸)(20);丁二酸(21);天冬氨酸(22);苯丙氨酸(23);缬氨酸(24);酪氨酸(25)。其中化合物1、3、4、7为从该植物中首次分离得到。
生物亲和色谱是利用自然界中存在的特异性相互作用生物物质对,通常具有很高的选择性,代表性物质有酶-底物、酶-抑制剂、激素互补接受体、抗体-抗原、药物-受体等。是利用生物活性物质的特异性相互作用进行生物样品分离分析和生物活性参数测定的新兴技术。本文建立了肝细胞固相色谱法并应用其对茵陈蒿汤的组方中茵陈蒿,栀子,大黄的效应成分进行分析。结果在茵陈蒿中得到四个效应物质的特征峰,通过各种分离手段及应用各种波谱数据鉴定了其中三个成分,分别为7-羟基-香豆素、7-甲氧基-香豆素、Capillartemisin A。在栀子中得到两个效应物质的特征峰,其中一个经与对照品共液相鉴定为栀子苷。
The water extracts from Artemisia capillaries Thurb and Morus alba L leaves were individually separated by modem chromatographic methods,such as silica gel col umn chromatography,Sephadex LH-20,TLC and semi-preparative reversed phase HPL C,and so on.The structures of compounds were elucidated on the basis of spectrosco pic methods(UV,IR,NMR,MS and CD) and their physical-chemical properties.
30 compounds were isolated from A.capillaries Thurb and were identified as 6'-O -caffeoyl-p-hydroxy-acetophenone-4-O-β-D-glucopyranoside(1);9-[1-(3,4- dihydroxyphe nyl)ethyl]benzene-adenine(2);3(R)-deca-4,6,8-triyne-l,3-diol(3);3(R)-decene-4,6-diyne-1, 3,8-triol(4);3(R)-9-deca-4,6,8-triyne-1,3-diol-1-O-β-D-glucopyranoside(5);7-hydroxycou marin(6);6,7-dimethoxycoumarin(7);6-methoxy-7-hydroxycoumarin(8);7-methoxycouma rin(9);4-hydroxyacetophenone(10);4-hydroxy-3-methoxyacetophenone(11);nicotinic aci d(12);benzenecarbinol-O-β-D- glucopyranoside(13);benzoic acid(14);oxalic acid(15); amber acid(16):quercetin(17);hyperoside(18);thymine(19);thymine ribonucleosi de(20);uracil(21);vanillin(22);vanillic acid(23);4-hydroxyacetophenone -4-O-β-D-glucopyranoside(24);2-hydro- benzoic acid(25);3-methoxy-caffeic acid a-4 -O-β-D-glucopyranoside(26);quercetin-7-β-D-glucopyranoside(27);isorhamnetin-3-O -β-D-glueopyranoside(28);loliolide(29);phthalic acid bis-(2-ethylhexyl) ester(30)。
25 compounds were isolated from M.alba leaves and were identified as acetyl-lol iolide(1);4-hydroxy- benzoic acid(2);dehydrololiolide(3);loliolide(4);o-dihydrox ybenzene(5):scopoletine(6);benzenecarbinol-O-β-D-glucopyranoside(7);3β-hydroxy-5α,6α-epoxy-7-megastimen-9-one(8);6,7-dihydroxycoumarin(9);cyclo-(Leu-Ile) (10);cyclo(Phe-Tyr)(11);3,4-dihydroxy-benzoic acid(12);isolarieiresinol(13);ci wujiatone(14);7,8-dihydroxycoumarin(15);cyclo(ala-leu)(16);vanillic acid-4-O-β-D-glucopyranoside(17);thymine(18);thymine ribonucleoside(19);quercetin(20); amber acid(21);aspartate(22);thenylalanine(23);valine(24);tyrosine(25)。
Bio affinity chromagraphy was a method which use some selective and specific pairs of substance existed in biological environment,such as enzyme-substrate,enzyme -inhibitor,hormone-receptor,antibody-antigen,medicine-receptor to analyze bio active s ubstance.Certain selective analysis and separation were archived by using these pairs. We established liver cell membrane solid phase chromatography in our reseach and pu t it into use in the analysis of active substance in Inchenhaotang.In result,we get 4 selected peaks in Artemisia capillaries Thurh and three of them were identified as 7-h ydroxycoumarin,7-methoxycoumarin and Capillartemisin A.2 peaks were obtained fro m Gardenia jasminoides Ellis and one of the them was identified as geniposide.
同时从桑叶的水提物中,分离得到31个单体化合物,通过理化性质和光谱分析,鉴定了其中25个化合物的结构,其中分别为acetyl-loliolide(1);对羟基苯甲酸(2);dehydrololiolide(3);loliolide(4);邻苯二酚(5);莨菪亭(6);苯甲醇-O-β-D-吡喃葡萄糖苷(7):3β-hydroxy-5α,6α-epoxy-7-megastimen-9-one(8);6,7-二羟基香豆素(9);环(亮氨酸-异亮氨酸)(10);槲皮素(11);3,4-二羟基苯甲酸(12);异落叶松脂素(13);刺五加酮(14);7,8-二羟基香豆素(15);环(丙氨酸-亮氨酸)(16):香草酸-4-O-β-D-葡萄糖苷(17);胸腺嘧啶(18);胸腺嘧啶脱氧核苷(19);环(苯丙氨酸-酪氨酸)(20);丁二酸(21);天冬氨酸(22);苯丙氨酸(23);缬氨酸(24);酪氨酸(25)。其中化合物1、3、4、7为从该植物中首次分离得到。
生物亲和色谱是利用自然界中存在的特异性相互作用生物物质对,通常具有很高的选择性,代表性物质有酶-底物、酶-抑制剂、激素互补接受体、抗体-抗原、药物-受体等。是利用生物活性物质的特异性相互作用进行生物样品分离分析和生物活性参数测定的新兴技术。本文建立了肝细胞固相色谱法并应用其对茵陈蒿汤的组方中茵陈蒿,栀子,大黄的效应成分进行分析。结果在茵陈蒿中得到四个效应物质的特征峰,通过各种分离手段及应用各种波谱数据鉴定了其中三个成分,分别为7-羟基-香豆素、7-甲氧基-香豆素、Capillartemisin A。在栀子中得到两个效应物质的特征峰,其中一个经与对照品共液相鉴定为栀子苷。
The water extracts from Artemisia capillaries Thurb and Morus alba L leaves were individually separated by modem chromatographic methods,such as silica gel col umn chromatography,Sephadex LH-20,TLC and semi-preparative reversed phase HPL C,and so on.The structures of compounds were elucidated on the basis of spectrosco pic methods(UV,IR,NMR,MS and CD) and their physical-chemical properties.
30 compounds were isolated from A.capillaries Thurb and were identified as 6'-O -caffeoyl-p-hydroxy-acetophenone-4-O-β-D-glucopyranoside(1);9-[1-(3,4- dihydroxyphe nyl)ethyl]benzene-adenine(2);3(R)-deca-4,6,8-triyne-l,3-diol(3);3(R)-decene-4,6-diyne-1, 3,8-triol(4);3(R)-9-deca-4,6,8-triyne-1,3-diol-1-O-β-D-glucopyranoside(5);7-hydroxycou marin(6);6,7-dimethoxycoumarin(7);6-methoxy-7-hydroxycoumarin(8);7-methoxycouma rin(9);4-hydroxyacetophenone(10);4-hydroxy-3-methoxyacetophenone(11);nicotinic aci d(12);benzenecarbinol-O-β-D- glucopyranoside(13);benzoic acid(14);oxalic acid(15); amber acid(16):quercetin(17);hyperoside(18);thymine(19);thymine ribonucleosi de(20);uracil(21);vanillin(22);vanillic acid(23);4-hydroxyacetophenone -4-O-β-D-glucopyranoside(24);2-hydro- benzoic acid(25);3-methoxy-caffeic acid a-4 -O-β-D-glucopyranoside(26);quercetin-7-β-D-glucopyranoside(27);isorhamnetin-3-O -β-D-glueopyranoside(28);loliolide(29);phthalic acid bis-(2-ethylhexyl) ester(30)。
25 compounds were isolated from M.alba leaves and were identified as acetyl-lol iolide(1);4-hydroxy- benzoic acid(2);dehydrololiolide(3);loliolide(4);o-dihydrox ybenzene(5):scopoletine(6);benzenecarbinol-O-β-D-glucopyranoside(7);3β-hydroxy-5α,6α-epoxy-7-megastimen-9-one(8);6,7-dihydroxycoumarin(9);cyclo-(Leu-Ile) (10);cyclo(Phe-Tyr)(11);3,4-dihydroxy-benzoic acid(12);isolarieiresinol(13);ci wujiatone(14);7,8-dihydroxycoumarin(15);cyclo(ala-leu)(16);vanillic acid-4-O-β-D-glucopyranoside(17);thymine(18);thymine ribonucleoside(19);quercetin(20); amber acid(21);aspartate(22);thenylalanine(23);valine(24);tyrosine(25)。
Bio affinity chromagraphy was a method which use some selective and specific pairs of substance existed in biological environment,such as enzyme-substrate,enzyme -inhibitor,hormone-receptor,antibody-antigen,medicine-receptor to analyze bio active s ubstance.Certain selective analysis and separation were archived by using these pairs. We established liver cell membrane solid phase chromatography in our reseach and pu t it into use in the analysis of active substance in Inchenhaotang.In result,we get 4 selected peaks in Artemisia capillaries Thurh and three of them were identified as 7-h ydroxycoumarin,7-methoxycoumarin and Capillartemisin A.2 peaks were obtained fro m Gardenia jasminoides Ellis and one of the them was identified as geniposide.
引文
[1]Isamu Okuno.Kiyohisa Uchida.Miham Nakamura et al SmdiesON Choleretic Constituents in Artemisia capillaris Thunb.[J]Chem Pharm Bull.1988.36(2):769- 775
[2]Indrani Chandrasekharan.Hamid A Khan.A.Ghanim.Flavonoidsfrom Artemisia scoparia [J].PlantaMedica,1981,43(3):310-311
[3]T.Nam ba.M.Hattori.Y.Takehana.et al.A Flavone from Artemisia capillaris[J].Phytochemistry.1983.22(4):1057-1058.
[4]Isao Kitagawa.Yoichi Fukuda.Minoru Yoshihara.et al.Capillanemisin A and B.two new cboleretic principles from Artemisiae capillaris herba.Chem Pharm Bull.1983,31(1):352-355.
[5]Fakeya Komiya.Yoshitomo Naruse.Haruji Oshio.Studies on"Inchinko".I Studies on the compounds related to capillarisinand flavonoids.Yakugaku Zasshi.1976.96(7)..855-862
[6]Yoshinobu Kiso.Shoko Ogasawara.Keiko Hirota.et al.Antihepatotoxic principles of Artemisia capillaris Buds[J].Planta Medica.1984.50(1):81-85.
[7]Mitsuo Miyazawa.Hiromu Kameoka.Neoeapillen,A new Acetylenic Hydrocarbon from Artemisia capillaris[J].Phytochemistry,1976,15:1988-1989.
[8]Mitsuo Miyazawa.Hiromu Kameoka.Norcapillene.A new acetylenic Hydrocarbon from the essential oil ofArtemisia capillaris.Phytochemistry,1976.15:223-224.
[9]Mitsuo M iyazawa.Hiromu Kameoka.Capillano1.A new Acetylemic AIcohol from Artemisia capillaris[J].Phytochemistry.1975.14:1874
[10]Rokuro Harada.Maroka l wasaki.Volatile components of Artemisia capillaris EJ].Phytochemistry.1982.2I(8):2009-2011
[11]junishi Veda.T'akao Yokota,Nobutuka Takahash1.et a l.A Root Growth promoting Factor,capillarol,from Artemisia capillaries Thunb[J].Asric Biol Chem,1986,50(12);3083-3086.
[12]Wu TS,ZhiH-Jing,Tsang,Wu PL.et al.Phenylalkynes from Artemisia capillaris[J].Phytochemisty.1998.47(8):1645-1648.
[13]张黎华等.中国中药杂志,1993,18(9):560
[14]白日淑等.国外医学中压中药分册.1997;19(1:27)
[15]Kiso Y.et al.Planta Med.1984j 50(1) 81
[16]贺平等.中国中药杂志,1990;15(8):52
[17]湖南医药工业研究所.中草药通讯,1972;(5):25
[18]Nomura M.et al.PhytotherRes,1995;9(8).563
[19]吴橙林等。江苏中药1996,17(5):17
[20]曾金凤等。中国中西医结合杂志1995,15(12):741
[21]张黎华等。中国中药杂志,1993,18(9):560
[22]蔡宝昌.国外医学中医中药分册1997{19(3):48
[23]杨彬等。中成药1996,18(4)34
[1].Bentley,Roger K.;Jones,Ewart R.H.;Thaller,Viktor.Polyacetylenes from Lactuca (lettuce) species of the Liguliflorae subfamily of theCompositae.Journal of the Chemical Society[Section]C:Organic(1969),(7),1096-1099.
[2].Stavri,Michael;Mathew,K.Thomas;Gibson,Trevor;Williamson,R.Thomas;Gibbons,Simon.Centre for.New constituents of Artemisia monosperma.Journal of Natural Products (2004),67(5),892-894.
[3].Naili Wang,Xinsheng Yao,Rie Ishii,and Susumu Kitanaka.Antiallergic agents form natural Sources.Structures and Inhibitory effect on nitric Oxide Production and histamine release of five novel poluacetylene glucosides from Bidens parviflora Willd.Chem pharm bull 2001,49(8),938-942.
[4].王倩,王丰,薛松等.茵陈中7-甲氧基香豆素的分离与含量测定[J].沈阳药科大学学报,2003,20(1):12-14.
[5].黄量,于德泉.紫外光谱在有机化学中的应用:下册[M].北京:科学出版社,1988.498.
[6].Sadtler Research Laboratories.Sadtler Standard NMR spectra[Z].Pennsylvania(USA):Sadtler Research Laboratories.1973.14671.
[7].Kitajima J,Ishikawa T,Tanaka Y,et al.Water-soluble constituents of Fennel.V.Glycosides of aromatic compounds[J].Chem PharmBull,1998,46(10):1587-1589
[8].黄量,于德泉.紫外光谱在有机化学中的应用:下册[M].北京:科学出版社,1988.498.
[9].Huang HB,Bao WF,Yang FF,et al.A study on chemical constituents of Erigemn breviscapus(Vant.)Hand-Mazz.JShenyang Pharm Univ,2001,182:206-208.
[10].Chen S B.Wang L W.Liao M C,et al.Studies on chemical constituents from Aquilegia ecalcarata Maxim(I)[J].China JMaterMed.2001,26(7):472-474.
[11].Li GY,Li BG,Liu GY,et al.Metabolites ofRhizopus arrhious 3078.NatProdRes Dev,2004,16:277-280.
[12].Alan J J,David MG,Michae WW,et al.Carbon-13 magnetic resonacne XVII pyrimidine and purine nucleosides.,lAmer Chem Soc,1970,92:4079.
[13].Li GY,Li BG,Liu GY,et al.Metabolites ofRhizopus arrhious 3078.NatProdRes Dev,2004,16:277-280.
[14].张庆英,赵玉英,刘雪辉,等.生腾化学成分研究[J].中国中药杂志,2000,25(2):101-103.
[15].王景祥,贡瑞生,黄土改,白蒿抗菌有效成分的研究[J],药学通报,1983,18(2):91.
[16].Kunijiro Yoshitama.Caffeic acid 4-[beta]-glucoside as the acyl moiety of the Senecio cruentus anthocyanin.Phytochemistry.1981,20,186-187
[17].Liu Q,Liu M,Tom JM,et al.Flavonol glycosides from Cephalocereus senilis [J].Phytochemistry,1994,36(1):229-231.
[18].Zhang CZ,Xu XZ,Li C.Fuctosides from Cynomorium songaricm[J].Phytochemistry,1996,41(3):975-976.
[19].KIMURA J,MAKI N.New loliolide derivatives from the Brown Alga Undaria pinnatifida[J].JNat Prod,2002,65(1):57-58
[20].Shi W,Han G;Chemical constituents of Tussilago farfara L[J].J Chin Pharm Sci,1996,5(2):63-67
[21].Dommisse RA,Van HL,Vlietinck AJ.Structural analysis of phenolic glucosides from salicaceae by NMR spectroscopy[J].Phytochem,1986,25(5):1201-1204.
[1]植飞,孔令义,彭司勋.大蓟化学成分的研究.药学学报,2003,38
[2]林琦,赵霞,刘鹏,等.三七脂溶性化学成分的研究.中草药,2002,33(6):490
[3]Naili Wang,Xinsheng Yao,Rie Ishi.et al.Antiallergic agents from natural sources 3.Structures and inhibitory effects on nitri oxide production and histamine release of five novel polyacetylene glucosides from bidens parviflora willd..Chem.Pharm.Bull.49(8) 938-9429(2001)
[4]Resch M,Heilmarm J,Steigel A,et al.Further phenols and polyacetylenes from the rhizomes of Atractylodes lancea and their anti-inflammatory activity.Planta Med,2001,67(5):437
[5]刘斌,石任宾,杨春梅,等.祁州漏芦水煎液化学成分研究.北京中医药大学学报,2003,26(1):53
[6]Kwon H C.Lee K R.An acetylene and a monoterpene glycoside from Adenocaulon himalaicum.Planta Med,2001,67(5):482
[7]李莉娅,邓志威,李军,等.中国南海海绵 Cinachyrella australensis化学成分研究.北京大学学报(医学版),2004,36(1):12
[8]杨叶坤,高诚伟,邱明华,等.苍白秤钩风的炔酸成分.云南植物研究,1999,21(2):265
[9]Choi Y E,Ahn H,Ryu J H.Polyacetylenes from angelica sisas and their inhibitory activity on nitric oxide synthesis in activated macrophages.Biol Pharm Bull,2000,23(7):884
[10]Liu J H,Zschocke S,Reininger E,et al.Inhibitory effects of Angelica pubescens biserrata on 5-lipoxygenase and cyclooxygenase.Planta Med,1998,64(6):525
[11]原忠,赵梦飞,陈发奎,等.北沙参化学成分的研究.中草药,2002,33(12):1063
[12]Stavri M,Ford C H,Bucar F,et al.Bioactyive constituents of,4rtemisia monosperma.Phytochemistry, 2005,66(2):233
[13]Chang S L,Chang C L,Chang Y M,et al.Polyacetylenic compounds and butanol fraction from Bidens pilosa can modulate the differentiation of helper T cells and prevent autoimmune diabetes in non-obese diabetic mice.Planta Med,2004.70(11):1045
[14]马明,王建平,徐凌川.婆婆针化学成分的研究.中草药,2005,36(1):7
[15]Zidom C,Johrer K,Ganzera M.et al.Polyacetylenes from the Apiaceae vegetables carrot,celery,fennel,parsley,and parsnip and their cytotoxic activities.JAgric Food Chem.2005,53(7):2518
[16]Bohlmann F,Burkhardt T,Zdero C.Naturally OccurringAcetylenes.1973,P1-35,499-508,Academic Press.
[17]J.B.Harbome,B.L.Turner.Plant Chemosystematics.1984,P198-204,Academic Press,London
[18]王建平,秦红岩,张惠云,等.鬼针草提取成分对白血病细胞的体外抑制作用.中药材,1997,20(5):247
[19]Kim J Y,Lee K W.King S H.et al.Inhibitory effect of tumor cell proliferation and induction of G_2/M cell cycle arrest by panaxytriol.Planta Med.2002,68(2):119
[20]王泽剑,吴英理,林琦,等.人参炔醇对HL-60细胞体外诱导分化作用的研究.中草药2003,34(8):736
[21]王泽剑,陆阳,陈红专.人参炔醇对大鼠脑片不同类型损伤的影响.上海第二医科大学学报,2003,23(6):485
[22]王泽剑,陈红专,薛庆生,等.人参炔醇对大鼠原代培养的神经细胞过氧化氢损伤的影响.中草药2005,36(1):72
[23]Rollinger J M.Zidom C.Dobner M J,et al.Lignans,phenylpropanoids and polyacetylenes from ChaerophyllumaureumL(Apiaceae).ZNaturforsch,2003,58(7-8)..553
[24]Mitsuo Miyazawa,Hideo Shimamura,Rakesh C,et a/.Antimutagenic Activity of Falcarindiol from Peucedanum praeruptorum.Planta Med1982,44(3):129
[1].韩金玉,那平,元英进,亲和色谱纯化蛋白质新进展,色谱1996,14(6):447-450.
[2].黄晰,邱宗荫,免疫亲和色谱定向偶联方法的研究.重庆医科大学学报1999,24(2):132-134.
[3].Ikegawa S,Matsuura K,Sato T,et al.Enantios elective immuno-atTmity extraction for simultaneous determination of optically active bufur alolandits metabolites in human plasma by HPLC[J].J Pharm BiomedAnal,1998,17(1):1-9.
[4].Safaei R.Atarget of the HoxB5 gene from the mouse nervous system[J].Brain Res Dev Brain Res,1997,100(I ):5-12.
[5].Greaser CS,Feely S J,Houghton E,et al.Immunoaffinity chromatography combinedon-line with high-performance liquid chromatography-mass spectrometry for the determination of corticosteroids[J].J Chromatogr A,1998,794(1/2):37-43.
[6].Kussak A,Andersson B,Andersson K,et al.Detennination of a flatoxicol in human urine by immunoaffinity column clean-up and liquid chromatography[J].Chernosphere,1998,36(8):1841-1848.
[7].Kershnar E,Wu SY,Chiang CM.Immunoaffinity purification and ftmctional characterization of human transcription factor Ⅱ Hand RNA polymerise Ⅱ from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes[J].J Biol Chem,1998,237(51):34444-34453.
[8].Zhou D,Zou H,Ni J,et al.Membrane supports as the stationary phase in high-performance immunoaffinity chromatography[J].Anal Chem,1999,71(1):115-118.
[9].Phillips TM,Krum JM.Recycling immunoaffinity chromatography for multipleanalyte analysis in biological samples[J].J Chromatogr B Biomed Sci Appl,1998,715(1):55-63.
[10].Deinl I,Angermaier L,Franzelius C,et al.Simple high-performance liquid chromatographic column-switching technique for the on-line immunoaffinity extraction and analysis of flunitrazepam and its main metabolitesin urine[J].J Chromatogr B Biomed Sci,Appl,1997,704(1/2):251-258.
[11].Suarez AM.Azcona JL.Rodriguez JM,et al.One-step purification of raisin A by immunoailinity chromatography[J].Appl Environ Microbiol,1997,63(12):4990-4992.
[12].Shelves WL,Larsen GL,Huwe JK.Use of anim-muno affinity column for tetrachlorodibenzo-p-dioxin serum sample cleanup[J].J Chromatogr B Biomed Sci Appl,1998,705(2):261-268.
[13].Liang X,Lubman DM,Rossi DT,et al.On-probe immuno affinity extraction by matrix-assisted laser desorption/ionization mass spectrometry[J].Anal Chem,1998,70(3):498-503.
[14].Strong RA,Cho BY,Fisher DH,et al.Immuno-detection approaches and high-performance immuno-aff'mity chromatography for ananalogue of bovine growth hormone releasing Factor attrace levels[J].Biomed Chromatogr,1996,10(6):337-345.
[15].Ruhn PF,Taylor JD,Hage DS.Determination of urinary albumin using high-performance immuno-affinity chromatography and flow injection analysis[J].Anal Chem,1994,66(23):4265-4271.
[16].Cole L J,Kennedy RT.Selective preconcentration for capillaryzone electrophoresis using protein G immunoaffinity capillary chromatography[J].Electrophoresis,1995,16(4):549-556
[17].Simon P,Delsaut P,Lafontaine M,et al.Automated column-switching high-performance liquidchromatography for the determination of aflatoxin[J].J Chromatogr B Biomed Sci,Appl,1998,712(1/2):95-104
[18].Deinl I,Angermaier L,Franzelius C,et al.Simple high-performance liquid chromatographic column-switching technique for theon-line immunoaffinity extraction and analysis of flunitrazepam and its main metabolites in urine[J].J Chromatogr B BiomedSci,Appl,1997,704(1/2):251-258.
[19].Kagel JR,Rossi DT,Nordblom GD,et al.Consideration in the development of a sensitive HPLC assay for human epidermal growth factors in human plasma[J].J pharm BiomedAnal,1995,13(10):1205-1213.
[20].Cai J,Hereon J.Quantitative mufti-residue determination of beta-agonists in bovine urine using on-line immunoaffinity extraction-coupled column packed capillary liquid chromatography-tandem mass spectrometry[J].J Chromatogr B Biomed Sci,Appl,1997,691(2):357-370
[21].Turesky RJ,Garner RC,Welti DH,et al.Meta-holism of the food-borne mutagen2-amino-3,8-dimethylimidazo[4,5-]quinoxaline in humans[J].Chem Res Toxicol,1998,11(3):217-225
[22]邹汉法,黄晓冬,生物分子的分离与检测,色谱,2003,21(4):311-315.
[23].Irving W.Wainer,Roman kaliszan and terence A.GNoctor.Biochromatograpl Using Immobilized Biopolymers:a new approach to he determination of pharmacological properties.J.Pharmacol.1993.45:307-373.
[24].Aubry.A.F.,McGann.A.Application of biochromatography to the determination of drug-protein binding interactions.LC-GC Int.1994,7:389-396.
[25].Domencici.E.,Bertucci,C.,Salvadori,P.,Wainer,1.W.(1991)The use of a human based HPLC chiral stationary phase for the investigation of protein bindin:the allosteric interaction between warfarin and benzo-diazepine binding sites.J.Pharm Sci.1991,80:164-166.
[26].Alleninark,S.Optical resolution by liquid chromatography on immobilized albumin.J.Liq.Chromato gr 1986,9:425-442
[27].Domenici,E.,Bertucci.,C.,Salvadori,P.,Felix,G.,Cahagnd,l.,Motellier,S.,Wainer,1.W.Synthesis and chromatographic oroperties of an HPLC chiral stationary phase based upon human serum albumin.Clmomatographia,1990,29:170-176.
[28].Hermansson,J.Direct liquid chromatographic resolution of racemic drug using α 1-acid glycoprotein as the stationary phase.J.Chromatogr.1983,269:71-80.
[29].Miwa,T.,Ichikawa,M.,Tsuno,M.,Hattori,T,Miyakawa,T,Kayanti,M.,Miyake,Y.Direct liquid chromatographic resolution of racemic compounds use of ovomucoid as a column ligand.Chem.Pharm.Bull.1987,35:682-686.
[30].Haginalca J.,Matsunaga H.Separation of enantiomers on HPLC chiral stationary phases
based on human plasma alphal-acid glycoprotein:effcct of sugar moiety on chiral recognition
ability.Enantiomer,2000,5(1):37
[31].Wainer,IW.,Jadaud,P.,Schonbaum,GR.,Kakookar.S.V.,Henry,M.P.Enzymes as HPLC supports for chiral resolutions:initial investigations with α-chymotrypsin.Chromatographic.1988,25:903-907.
[32].Thelohan,S.,Jadaud,P.,Wainer,I.W.Immobilized enzymes as chromatographic phase for HPLC:the chromatography of tree and derivatized amino acids on trypsin.Chromatographia,1989,28:551-555
[33].Chuff,W.K.,Wainer,l.W.(1992)Enzyme based HPLC supports as probes of enzyme activity and inhihitionahe immobilization of trypsin and α-chymotrypsin on an immobilized artificial membrane HPLC support.Anal.Biochem.1992,201:237-245.
[34].Labrou NE.Improved purification dehydrogenase.Bioseparation.2000;9(2):99-104.
[35].Walsh MK,Nam SH.Affinity enrichment of bovine lactoferrin in whey.Prep Biochem Biotechnol.2001,Aug;31(3):229-40.
[36].Noctor,T.A.G,Felix,G.,Wainer,I.W.Stereochemical resolution of enantiomeric 2-aryl propionic acid non-steroidal anti-inflammatory drug on a human serum album based high performance liquid chromatographic chiral stationary phase.Chromatographia.1991,31:55-59.
[37].Allenmark S.Chromatographic Enantioseparation:Methods and Applications.2nd Edition.New York:Ellis Horwood,1991,chapter 7.
[38].YANG Yongjian,Hou Meiqin.Enantiomeric separation of salmeterol by HPLC using αl-acid glycoprotein chiral stationary phase.Chin JPharm Anal,2001,21(2):97.
[39].Gyimesi-Forms K,Szasz G,Gergely A,et al.Optical resolution of a series of potential cholecystokinin antagonist 4(3H)-quinazolone dekivatives by chiral liquid chromatography on alphal acid glycoprotein stationary phase.J Chromatogr.Sci,2000,38(10):430.
[40].Jadaud P,Thelohan S,Schonbaum CR,et al.The stereochemical resolution of enantiomeric free and derivatized amino acids using an HPLC chiral stationary phase based structure on immobilized or enzymealpha-chymotrypsin:chiral separation due to solute activity.Chirality,1989,1(1):38.
[41].Henriksson H,Munoz IG,Isaksson R,et al.Cellobiohydrolase 58(P.c.Ce17D) is complementary to the homologouse CBHI(T.r.Cel7A) in enantioseparations.J Chromatogr.A.2000,898(1):63.
[42].Divne C,Stahiberg J,Teeri T,et al.High-resolution crystal structures reveal how a cellulose chain is bound in the 50A long tunnel of cellobiohydrolase I from Trichoderma reesei.J Mol Biol.1998,275(2):309.
[43].Mao N,Oda Y,Takakuwa S,et al.Plasma direct injection high-performance liquid chromatograpgic method for simultaneously determining E3810 enantiomers and their metabolites by using flavoprotein-conjugated column.J Pharm Sci,1996,85(9):903.
[44].Vermeulen B,Remon JP.Validation of a high-performance liquid chromatographic method for the determination of ibuprofen enantiomers in plasma of broiler chickens.J Chromatogr(Netherlands),2000,749(2):243.
[45].Ceccato A,Hubert P,de Tullio P,et al.Simultaneous determination of pirlindole enantiomers and dehydropirlindol by chiral liquid chromatography.J Pharm Biomed Anal,1998,17(67):1071.
[46].Domencici.E.,Bertucci,C,Salvadori,P,Felix,G,Cahagnd,I,Motellier,S.,Wainer,LW.Lmmobilized serum alburnin:a rapid probe of stereoselective protein binding interactions.Chirality.1990,2:263-268.
[47].Domencici.E.,Bertucci,C,SaIvadori,R,Wainer,LW.The use of a human serum albumin based HPLC chiral stationary phase for the investigation of protein binding:the detection of the allosteric interaction between warfarin and benzo-diazepine binding sites.J.Pharm Sci.1991,80:164-166.
[48].Noctor,T.A.G,Hage.D.S.,Wainer,1.W.(1992).Human serum albumin based chiral stationary phase for high performance liquid chromatography:a tool for the rapid study of drug-protein binding interactions.J.Chromatogr,577:305-315.
[49].Noctor,T.A.G,Diaz-Perez,MJ.,Wainer,I:W.(1993).The use of a human serum albumin based stationary phase for-high performance liquid chromatography as a tool for the rapid determination of drug-plasma protein binding.J.Pharm.Sci.1993.
[50].Kaliszan,R.,Noctor.T.A.G,Wainer,IW.Stereochemical aspects of benzodiazepine binding to human serum album.II.Quantitative relationships between structure and enantioselective retention in high performance liquid chromatography.Mol.Pharmacol. 1992,42:512-517.
[51].Noctor,T.A.G.,Cuong,D.P.,Kaliszan,R.,Wainer,I.W.Stereochemical aspects of benzodiazepine binding to human serum albtrm.I.Enantioselective high performance liquid chromatography examination of chiral binding interactions between 1,4-BDZ and human serum albumin.Mol.Pharmacol.1992,42:506-511.
[52].Das,A.S.,Singh,S.,Raghubanshi,R.S.,Jayalswal.S.B.,Singh,J.Effect of drugs in vitro captopril protein binding.Phannazie.1989,44:503-504.
[53].Iieire,D.A.,Santhana Mariappan,S.V.,Peng,J.,Rabenstein,D.L.Nuclear magnetic studies of the binding of captopril and penicillamin by serum albumin.Biochem.Pharmacol.1993,46:1059-1069.
[54].Anne,Francoise Aubry,et al.The effect of Co-administered drugs on Oxaprozin binding to human serum albumin.1995,47:937-944.
[55].Kaliszan R,Nasal A,Turowslcim.Binding site for basic drugs on aiphal-acid glycoprotein a by chemometric anylysis of biochromatographic data.Biomed Chromatogr:1995,9(5):211-215.
[56]孔亮,邹汉法,汪海林等.以人血清白蛋白为固定相的分子生物色谱分析几种中药活性成分的研究[J].高等学校化学学报,2000,21(1):3640.
[57].Wang HL,Kong L,Zou HF,et al.Screening and analysis of biologically active compounds in Angelica sinensis by molecular biochromatography[J].Chromatographic,1999,50(7/8):394-445.
[58].Wang H,Zou H,Ni J,et al.Comparative separation of biologically active components in Rhizoma chuanxiong by aff'mity chromatography with α_1-acid glycoprotein and human serum albumin as stationary phases[J].Chromatographic,2000,52(718):459-464.
[59].Wang H,Zou H,Ni J,et al.Fractionation and analysis ofArtemisia capillaris Thunb.by affinity chromatography with human serum albumin as stationary phase[J].J Chromatogr A,2000,870:501-510.
[60].Wang H,Zou H,Kong L,et al.Analysis of bioactive components in traditional Chinese medicines by molecular biochromatography with α＿1-acid glycoprotein as stationary phase [J].Basic Clin Physiol Pharmacol;2000,11(2):155-172.
[61].汪海林,邹汉法,孔亮等.分子生物色谱用于中药活性成分筛选及质量控制方法的研 究[J].色谱1999,17(2):123-127
[62].Hailin Wang,Liang kong,Hanfa Zou,et al.Screening and analysis of biologically active compounds in Angelica sinensis by Molecular biochromatography.Chromatographic.Vol 50.No.718.October.1999.439-445.
[63].Wang H,Zou H,Kong L,Ni J.Analysis of bioactive components on traditional Chinese medicines by molecular biochromatography with alphal-acid glycoprotein stationary phase.Basic Clin physiol Pharmacol.2000:11(2):155-172.
[64]郭明,孔亮,厉欣,邹汉法.微透析探针的制备及其在Ni(Ⅱ)与人血清白蛋白的相互作用研究中的应用色谱,2003,21(2):147.
[65]毛希琴,邹汉法,封顺,孔亮,黄晓冬,厉欣,罗权舟.3种色谱模式联用在中药活性成分初步筛选中的应用.2003分析化学,31(8):992-995.
[66]汪海林,邹汉法,孙生才,张玉奎微透析取样技术及其在生化分析中的应用。色谱,1996.14(6):441.
[67].Cai S J,McAndrew R S,Leonard B P,et al.Rapid purification of cotton seed membrane-bound N-acylphosphatidylethanolamine synthase by immobilized artificial membrane chromatography[J].J Chromatogr A,1995,696:49-62.
[68].Yang Q,Wallsten M,Lundahl P.Lipid-vesicle-surface chromatography[J].1 Chromatogr,1990.506:379-389.
[69].Yang Q,Lundahl P.Binding of lysozyme on the surface of entrapped phosphatidylserine-phosphatidylcholine vesicles and an example of high-performance lipid vesicle surface chromatography[J].J Chromatogr,1990,512:377-386.
[70].Pidgeon C,Cai S J,Bemal C.Mobile phase effects on membrane protein elution during immobilized artificial membrane chromatography fJ].J Chromatogr A,1996,721:213-230.
[71]Hanna M,Biasi V D,Bond B,et al.Biomembrane lipids as components of chromatographic phases:comparative chromatography on coated and bonded phases[J].Chromatographic,2000.52(11/12):710-720.
[72].Lundahl P,Yang Q.Liposome chromatography:liposomes immobilized in gel beads as a stationary phase for aqueous column chromatography[J].J Chromatogr,1991,544:283-304.
[73].Klein E.Affinity membranes:a 10-year review[J].J Membr Sci,2000,179(1-2):1-27.
[74].毛希琴,邹汉法,罗权舟等.模拟生物膜色谱用于预测药物的小肠吸收[J]_分析化学, 2001,29(10):1135-1139.
[75].Pidgeon C,Venkataram U.V.Immobilized artificial membrane chromatography:supports composed of membrane lipids[J].Anal Biochern,1989,176:36-47.
[76].毛希琴,邹汉法,罗权舟,孔亮,厉欣,孙乃昌.卵磷脂涂敷生物膜色谱固定相的制备及其稳定性的考察。色谱,2001,19(5):433.
[77].贺浪冲,杨广德,耿信笃,固定在硅胶表而细胞膜的酶活性及其色谱特性。科学通报,1999,44(6):632-637.
[78].He LC,Wang SC,Geng XD.Coating and fusing cell membrane onto a silica surface and their chromatographic characteristic[J].Chromatographia,2001,54:77.
[79]He LC,Yang GD,Geng XD.Enzymatic and chromatophic characteristics of the cell membrane immobilized on silica surface.Chinece Science Bulletic,1999,44(9):826.
[80].Brekkan E,Lundqvist A,Lundahl P.Immobilized membrane vesicle or proteoliposome affinity chromatography.Frontal analysis of interaction of cytochatasin L and D-glucose with the human red cell glucose transporter[J].Biochern,1996,35:12141-12145.
[81].Beigi E Lundahl P.Immobilized biomembrance chromatography of highly lipophilic drugs[J].J ChromatogrA,1999,852:313-317.
[82].董自波,红细胞膜生物色谱法的建立及其在当归效应物质研究中的应用。南京中医药大学博士研究生论文,2002.5.
[83].张汉利,杨广德,贺浪冲,杨银京。太白花活性成分的筛选与药理作用相关性研究。中国药学杂志2003,38(2):92-94
[84].赵小娟,党高潮,杨广德,贺浪冲.淫羊蕾根活性成分的分析和比较[J].分析化学2002,30(2):195-197.
[85].高现,贺浪冲,杨广德.用细胞膜色谱法筛选研究红毛七中的有效成分.中国药学杂志2003,38(1):14-16.
[86].赵惠茹,杨广德,贺浪冲.用细胞膜色谱法筛选当归中的有效成分[J].中国药学杂志2000,35(1):13-15.
[87].贺浪冲,耿信笃.细胞膜受体色谱法研究药物与受体作用的新方法[J].生物医药色谱新进展,1996,3:8.
[88].HOU Jin,YIJAN,BINGXiang,HE Lang Chong,et al,Evaluation of drug muscarinic receptor affinities by using cell membrane chromatography.Chin J Pharmacol Toxico l.2003,17(1):70-73.
[89].HE Lang-chong,WANG Si-cen,GENG Xin-du.Coating and fusing cell membrane onto a silica surfuse and their chromatographic characteristics.Chromatographia,2001,54-71,
[90].Zeng C M,Zhang Y X,Lu L L,et al.Immobilization of human red cells in gel particles for chromatographic activity studies of the glucose transporter Glut I[J].Biochem Biophys Acta,1997,1325(1):91-98.
[91].Muszynska G,Andersson L,Porath J.Selective adsoption of phosphoproteins on gel-immobilized ferricchelate.Biochemistry,1986,25(22):6850-6853.
[92].Zhou W,Merrick BA,Khaledi MG,Tomer KB.Detection and sequencing of phosphopeptide aff'mity bound to immobilized metal ion beads by matrix assisted desorption/ionization mass spectrometry.Jam Soc Mass Spectrom,2000,11(4):273-282.
[93].Stensballe A,Andersen S,Jensen ON.Characterization of phosphoproteins from electrophoretic gels by nanoscale Fe(Ⅲ) affinity chromatography with off-line mass spectrometry analysis.Proteornics,2001,1(2):207-222.
[94].大连理工大学无机化学教研室,无机化学,第三版—北京,高等教育出版社,1990,349:384.
[95].Ueda EK,Gout PW,Morganti L.Ni(Ⅱ)-based immobilized metal ion affinity chromatography of recombinant human prolactin from periplasmic Escherichia coli extracts.J ChromatogrA.2001 Jul 13;922(]-2):165-75.
[96].程慧文,邵天梦,鳌合金属离子亲和色谱法分离α-氨基酸和肽[J],色谱1997,15(5):405-407.
[97].邸泽梅,陈国亮,雷建都,李蓉,李华儒.金属鳌合亲和色谱介质的合成及其色谱特性研究,色谱1998,16(4):297-300.
[98].李蓉,邸泽梅,陈国亮,金属鳌合亲和色谱中固定金属与蛋白质的作用,分析化学2002,30(5):552-555.
[99],Slentz BE,Penner NA,Regnier.Protein protedysis and the multi dimensional electrochromatographic separation of histidine containing peptide fragments on a chip.J Chromatogr A.2003,984:97.
[100].Riggs L,Sioma C,Regnier FE.J ChromatogrA,2001,924:359.
[101].Luo QZ,Zou HF,Zhang Q,Xiao XZ et aI.J ChromatogrA,2001,926:255
[102].Larsen MR,Soren GL.,Fey SJ,Larsen PM,Roepstorff E Phosphoproteomics:Evalution of the use of enzymatic dephosphorylation and differential mass spectrometric peptide mass yapping for site specific phosphorylation assigrrmentin proteins separated by gel electrolhresis.Proteomics,2001,1(2):223-238.
[103].McLachlin DT,Chait BT.Analysis of phosphorylated proteins and peptides by mass spectrometry.Curr Opin Chem Biol,2001,5(5):591-602.
[104].王京兰,张养军,蔡耘,李蕾,刘尚义,杨何义,钱小红.生物质谱结合 IMAC 亲和提取和磷酸酶水解分析蛋白质磷酸化修饰。生物化学与生物物理学报2003,35(5):459-466.
[105].高勤,左文坚。免疫亲和色谱及其在生物样本分析中的应用,国外医学药学分册2000,27(2):107-111
[106].叶明亮,邹汉法,刘震,雷政登,倪坚毅.开管毛细管亲和液相色谱初探.色谱,2000,18(6):529-531
[107].何新亚,林炳承,丁永生。亲和毛细管电泳研究生物分子的手性识别。化学进展,2002;14(5):332.
[108].潘再法,莫卫民,吴仁安.蛋白 A 连续床亲和毛细管色谱柱的制备及性能考察,高等学校化学学报,2002,23(11):2070-2072.
[109].Rocklin RD,Rainsey RS,Ramsey JM.A microfabricated fluidic device for performing two-dimensional liquidic-phase separations.Anal Chem,2000,72:5244-5247.
[110].Zhang X,Hu X,XuS,et al.Comprehensive two-dimensionalcapillaryLC and CEfor resolution of neutral components in traditional Chinese medicines.J Sep Sci 2001,24:385-391.
[1]苏海涯,吴越明,刘建新.桑叶中的营养物质和生物活性物质[J].饲料研究,2001
[2]朱祥瑞,陆洪省.桑叶化学成分及其药用价值[J].全国桑柞茧丝新资源开发利用研讨会论文集,2006
[3]金丰秋。新型功能性饮品—桑茶[J].食品科学,2000,21(1):46
[4]Kim Sy.Antionxiative Flavonoids from the leaves ofMoms alba L.[J].Arch Pharm Res,1999,22(1):81
[5]Kayo Doi.Studies on the constituent of the leaves of Moms alba L.[J].Chem Pharm Bull,2001,49(2):151-153
[6]陈福君,卢军,张永煜.桑的药理研究(I):桑叶降糖有效组分对糖尿病动物代谢的影响[J].沈阳药科大学学报,1996,13(1):24
[7]张琳华,高瑞旭,许明丽.桑叶中多糖提取分离工艺的研究[J].中草药,2005,36(4)
[8]Yoshiaki Yoshikuni.Inhibition of α-glucosidase activity and postprandial hyperglycemia by moraline and its N-alkyl derivatives[J].Agric Biol Chem.,1988,52(1):121-128
[9]Asano N.Sugars with Nitrogen in the Ring Isolated in the Leaves ofMoms Bombycis[J].Carbohydr Res,1994,253:235
[10]邹盛勤,陈武.桑叶的化学成分、药理活性及应用研究进展[J].林业加工通讯,2003,37(1):22-25
[11]王芳,励建荣。桑叶的化学成分、生理功能及应用研究进展[J].食品科学,2005,26(增刊):111
[12]Arai M,Minatoguehi S,Takemure G,et.N-methyl-l-deoxynojirimycin(MOR-14),an α-glucosidase inhibitor,markedly reducedinfarct size in rabbit hearts[J],Circulation.1998,97(13):1290-1293[13]Kimura M,Chen F.Antihyperglycemic effects of N-containing sugar derived from mulberry leaves in strep tozocin-induced diabetic mice[J],Wakan Iyakugaku Zasshi,1995,12(3):214-219
[14]TaniguchiS.AsanoN.TominoF.et al.Polyhydroxylated of glucose-induced insulin secretion by fagomine,a pseudo-sugar isolatedfrom mulberry leaves[J].Horm Metab Res.1998,30(1):676-683
[15]贾之慎.唐孟成,朱祥瑞.桑叶黄酮类化合物清除超氧离子自由基的研究[J].浙江农业大学学报.1996.22(5):519-523
[16]Doi K,Kojima T,Fujimoto Y.Mulberry leaf extract inhibits the oxidative modidication of rabbit and human low density lipoprotein[[J].BiolPharm Bull.2000,23(9):1066-1071
[17]武深秋.养生保健话桑叶[J].药膳食疗,2003,(1):76
[18]居跃君,袁茂云,吴秀玲.平肝安神汤治疗高血压病伴失眠症102例[J].辽宁中医杂志,2003,30(7):548-549
[19]孙秀凤.桑菊饮加味治疗喉源性咳嗽60例[J].陕西中医,2003,24(4):305-306
[20]颜治,屠大伟,郑诗超等.桑叶碳酸饮料的制作工艺研究[J].广州食品工业科技.2003,19(2):49-50
[21]李莉.桑叶保健酒的研制[J].湖北民族学院学报(自然科学版).1997,15(6):34-35
[22]One K.Inhibition of reverse transcriPtase activity by a flavonoid compound 5,6,7-trihydroxynavone.Biochem.Bio-Phys.Res.Commun.1989,160(3):982-984
[23]Groweiss A.桑科植物Maclura tinctoria中具有抗HIV活性的异戊烯基山酮和黄酮类化合物。国外医药-植物药分册,2001,16(6):261
[24]Kim S.Y.Gao J.J,Kang H.K.Two flavonoids from the leaves ofMorus alba induce differentiation of the human promyelocytic leukemia(HL-60)cell line[J].Biolpharm Bull,2000,23(4):451-455
[25]华晓珊,两地汤加减治疗室女崩漏47例[J],陕西中医,2001,22(6):329
[26]陈军,王明智,治疗放射性肺炎的润肺通络法[J].江南大学学报(自然科学版).2002,1(4):435-436
[27]江苏新医学院,中药大辞典(下册)[M],上海:上海人民出版社,1997、
[28]金丰秋,金其荣,新型功能性饮品—桑茶[J],食品科学,2000,21(1):46
[1]HANSJORG D1ETZ and PETER WINTERHALTER.Phytotoxic constituens form Bunias orientalis leaves.Phytochemistry,Vol.42,No.4,pp.1005-1010,1996
[2]Maclas,F.A.,Oliva.et aL Allelochemicals from sunflower leaves cv.Peredovick.Phytochemistry 1999(52),613-621.
[3]KIMURA J,MAKI N.New loliolide derivatives from the Brown Alga Undaria pinnatifida[J].JNat Prod,2002,65(1):57-58
[4]Oliver K,Herbert K.Highly oxygenated coumarins from Pelargonium sidoides[J].Phytochemistry,1995,39(5):1181
[5]Kitajima J,Ishikawa T,Tanaka Y,et al.Water-soluble constituents of Fennel.V.Glycosides of aromatic compounds[J].ChemPharmBull,1998,46(10):1587.
[6]Hongquan Duan,Yoshihisa Takaishi,,Hiroshi Momota.et al.Immunosuppressive constituents from Saussurea medusa.Phytochemistry 59(2002) 85-90
[7]Tan J J,Jiang S H,Zhu D Y.Studies on the chemical constituents ofPleurospermum lindleyanum [J].Nat ProdResDev,2005,17(3):267-271.
[8]程志红,吴瞍,余伯阳.麦冬块根化学成分的研究,天然产物研究与开发,2005,17(1),1-3
[9]程志红,吴搜,余伯阳.冬块根化学成分的的研究[J].无然产物研究与开发,2005,17(1):1.
[10]王双明,谭宁华,杨亚滨,等.七环二肽成分[J].无然产物研究与开发,2004,16(5):383.[11]Agrawal PK,Rastogi RPR.~(13)CNMR spectral analysis of dihydmbenzofuran lignans[J].Org Res,1983,21(2):119-121.
[12]Thomas P,OlofT.The constituents ofc0nifer needles.VI:Phenolic glycosides from Pinus sylvestris [J].ActaChemScandB,1977,31:329-337.
[13]吴立军,郑健,姜宝虹等.刺五加茎叶化学成分.药学学报,1999,34(4):294-296
[14]刘桂芳,王连.瑞香狼毒化学成分研究[J].中国中药杂志20(12),1995
[15]向兰,郭东晓,鞠瑞等.马齿苋中环二肽成分研究[J].中草药,2007,38(11)1622-1625
[16]SU Dong-min,TANG Wen-zhao,YU Shi-shan,et al.Water-soluble constituents from roots of Capparis tenera.China journal of Chinese material medica.33(9),1021-1023
[17]Alan J J,David MG,Michae WW,et al.Carbon-13 magnetic resonacne XVII pyrimidine and purine nucleosides.JAmer Chem Soc,1970,92:4079.
[18]Sadtler Research,Laboratories Standard infrared grating spectra[M].Philadelphia USA.Sadtler Research Laboratorieies Lnc.1988,67,491-493
[2]Indrani Chandrasekharan.Hamid A Khan.A.Ghanim.Flavonoidsfrom Artemisia scoparia [J].PlantaMedica,1981,43(3):310-311
[3]T.Nam ba.M.Hattori.Y.Takehana.et al.A Flavone from Artemisia capillaris[J].Phytochemistry.1983.22(4):1057-1058.
[4]Isao Kitagawa.Yoichi Fukuda.Minoru Yoshihara.et al.Capillanemisin A and B.two new cboleretic principles from Artemisiae capillaris herba.Chem Pharm Bull.1983,31(1):352-355.
[5]Fakeya Komiya.Yoshitomo Naruse.Haruji Oshio.Studies on"Inchinko".I Studies on the compounds related to capillarisinand flavonoids.Yakugaku Zasshi.1976.96(7)..855-862
[6]Yoshinobu Kiso.Shoko Ogasawara.Keiko Hirota.et al.Antihepatotoxic principles of Artemisia capillaris Buds[J].Planta Medica.1984.50(1):81-85.
[7]Mitsuo Miyazawa.Hiromu Kameoka.Neoeapillen,A new Acetylenic Hydrocarbon from Artemisia capillaris[J].Phytochemistry,1976,15:1988-1989.
[8]Mitsuo Miyazawa.Hiromu Kameoka.Norcapillene.A new acetylenic Hydrocarbon from the essential oil ofArtemisia capillaris.Phytochemistry,1976.15:223-224.
[9]Mitsuo M iyazawa.Hiromu Kameoka.Capillano1.A new Acetylemic AIcohol from Artemisia capillaris[J].Phytochemistry.1975.14:1874
[10]Rokuro Harada.Maroka l wasaki.Volatile components of Artemisia capillaris EJ].Phytochemistry.1982.2I(8):2009-2011
[11]junishi Veda.T'akao Yokota,Nobutuka Takahash1.et a l.A Root Growth promoting Factor,capillarol,from Artemisia capillaries Thunb[J].Asric Biol Chem,1986,50(12);3083-3086.
[12]Wu TS,ZhiH-Jing,Tsang,Wu PL.et al.Phenylalkynes from Artemisia capillaris[J].Phytochemisty.1998.47(8):1645-1648.
[13]张黎华等.中国中药杂志,1993,18(9):560
[14]白日淑等.国外医学中压中药分册.1997;19(1:27)
[15]Kiso Y.et al.Planta Med.1984j 50(1) 81
[16]贺平等.中国中药杂志,1990;15(8):52
[17]湖南医药工业研究所.中草药通讯,1972;(5):25
[18]Nomura M.et al.PhytotherRes,1995;9(8).563
[19]吴橙林等。江苏中药1996,17(5):17
[20]曾金凤等。中国中西医结合杂志1995,15(12):741
[21]张黎华等。中国中药杂志,1993,18(9):560
[22]蔡宝昌.国外医学中医中药分册1997{19(3):48
[23]杨彬等。中成药1996,18(4)34
[1].Bentley,Roger K.;Jones,Ewart R.H.;Thaller,Viktor.Polyacetylenes from Lactuca (lettuce) species of the Liguliflorae subfamily of theCompositae.Journal of the Chemical Society[Section]C:Organic(1969),(7),1096-1099.
[2].Stavri,Michael;Mathew,K.Thomas;Gibson,Trevor;Williamson,R.Thomas;Gibbons,Simon.Centre for.New constituents of Artemisia monosperma.Journal of Natural Products (2004),67(5),892-894.
[3].Naili Wang,Xinsheng Yao,Rie Ishii,and Susumu Kitanaka.Antiallergic agents form natural Sources.Structures and Inhibitory effect on nitric Oxide Production and histamine release of five novel poluacetylene glucosides from Bidens parviflora Willd.Chem pharm bull 2001,49(8),938-942.
[4].王倩,王丰,薛松等.茵陈中7-甲氧基香豆素的分离与含量测定[J].沈阳药科大学学报,2003,20(1):12-14.
[5].黄量,于德泉.紫外光谱在有机化学中的应用:下册[M].北京:科学出版社,1988.498.
[6].Sadtler Research Laboratories.Sadtler Standard NMR spectra[Z].Pennsylvania(USA):Sadtler Research Laboratories.1973.14671.
[7].Kitajima J,Ishikawa T,Tanaka Y,et al.Water-soluble constituents of Fennel.V.Glycosides of aromatic compounds[J].Chem PharmBull,1998,46(10):1587-1589
[8].黄量,于德泉.紫外光谱在有机化学中的应用:下册[M].北京:科学出版社,1988.498.
[9].Huang HB,Bao WF,Yang FF,et al.A study on chemical constituents of Erigemn breviscapus(Vant.)Hand-Mazz.JShenyang Pharm Univ,2001,182:206-208.
[10].Chen S B.Wang L W.Liao M C,et al.Studies on chemical constituents from Aquilegia ecalcarata Maxim(I)[J].China JMaterMed.2001,26(7):472-474.
[11].Li GY,Li BG,Liu GY,et al.Metabolites ofRhizopus arrhious 3078.NatProdRes Dev,2004,16:277-280.
[12].Alan J J,David MG,Michae WW,et al.Carbon-13 magnetic resonacne XVII pyrimidine and purine nucleosides.,lAmer Chem Soc,1970,92:4079.
[13].Li GY,Li BG,Liu GY,et al.Metabolites ofRhizopus arrhious 3078.NatProdRes Dev,2004,16:277-280.
[14].张庆英,赵玉英,刘雪辉,等.生腾化学成分研究[J].中国中药杂志,2000,25(2):101-103.
[15].王景祥,贡瑞生,黄土改,白蒿抗菌有效成分的研究[J],药学通报,1983,18(2):91.
[16].Kunijiro Yoshitama.Caffeic acid 4-[beta]-glucoside as the acyl moiety of the Senecio cruentus anthocyanin.Phytochemistry.1981,20,186-187
[17].Liu Q,Liu M,Tom JM,et al.Flavonol glycosides from Cephalocereus senilis [J].Phytochemistry,1994,36(1):229-231.
[18].Zhang CZ,Xu XZ,Li C.Fuctosides from Cynomorium songaricm[J].Phytochemistry,1996,41(3):975-976.
[19].KIMURA J,MAKI N.New loliolide derivatives from the Brown Alga Undaria pinnatifida[J].JNat Prod,2002,65(1):57-58
[20].Shi W,Han G;Chemical constituents of Tussilago farfara L[J].J Chin Pharm Sci,1996,5(2):63-67
[21].Dommisse RA,Van HL,Vlietinck AJ.Structural analysis of phenolic glucosides from salicaceae by NMR spectroscopy[J].Phytochem,1986,25(5):1201-1204.
[1]植飞,孔令义,彭司勋.大蓟化学成分的研究.药学学报,2003,38
[2]林琦,赵霞,刘鹏,等.三七脂溶性化学成分的研究.中草药,2002,33(6):490
[3]Naili Wang,Xinsheng Yao,Rie Ishi.et al.Antiallergic agents from natural sources 3.Structures and inhibitory effects on nitri oxide production and histamine release of five novel polyacetylene glucosides from bidens parviflora willd..Chem.Pharm.Bull.49(8) 938-9429(2001)
[4]Resch M,Heilmarm J,Steigel A,et al.Further phenols and polyacetylenes from the rhizomes of Atractylodes lancea and their anti-inflammatory activity.Planta Med,2001,67(5):437
[5]刘斌,石任宾,杨春梅,等.祁州漏芦水煎液化学成分研究.北京中医药大学学报,2003,26(1):53
[6]Kwon H C.Lee K R.An acetylene and a monoterpene glycoside from Adenocaulon himalaicum.Planta Med,2001,67(5):482
[7]李莉娅,邓志威,李军,等.中国南海海绵 Cinachyrella australensis化学成分研究.北京大学学报(医学版),2004,36(1):12
[8]杨叶坤,高诚伟,邱明华,等.苍白秤钩风的炔酸成分.云南植物研究,1999,21(2):265
[9]Choi Y E,Ahn H,Ryu J H.Polyacetylenes from angelica sisas and their inhibitory activity on nitric oxide synthesis in activated macrophages.Biol Pharm Bull,2000,23(7):884
[10]Liu J H,Zschocke S,Reininger E,et al.Inhibitory effects of Angelica pubescens biserrata on 5-lipoxygenase and cyclooxygenase.Planta Med,1998,64(6):525
[11]原忠,赵梦飞,陈发奎,等.北沙参化学成分的研究.中草药,2002,33(12):1063
[12]Stavri M,Ford C H,Bucar F,et al.Bioactyive constituents of,4rtemisia monosperma.Phytochemistry, 2005,66(2):233
[13]Chang S L,Chang C L,Chang Y M,et al.Polyacetylenic compounds and butanol fraction from Bidens pilosa can modulate the differentiation of helper T cells and prevent autoimmune diabetes in non-obese diabetic mice.Planta Med,2004.70(11):1045
[14]马明,王建平,徐凌川.婆婆针化学成分的研究.中草药,2005,36(1):7
[15]Zidom C,Johrer K,Ganzera M.et al.Polyacetylenes from the Apiaceae vegetables carrot,celery,fennel,parsley,and parsnip and their cytotoxic activities.JAgric Food Chem.2005,53(7):2518
[16]Bohlmann F,Burkhardt T,Zdero C.Naturally OccurringAcetylenes.1973,P1-35,499-508,Academic Press.
[17]J.B.Harbome,B.L.Turner.Plant Chemosystematics.1984,P198-204,Academic Press,London
[18]王建平,秦红岩,张惠云,等.鬼针草提取成分对白血病细胞的体外抑制作用.中药材,1997,20(5):247
[19]Kim J Y,Lee K W.King S H.et al.Inhibitory effect of tumor cell proliferation and induction of G_2/M cell cycle arrest by panaxytriol.Planta Med.2002,68(2):119
[20]王泽剑,吴英理,林琦,等.人参炔醇对HL-60细胞体外诱导分化作用的研究.中草药2003,34(8):736
[21]王泽剑,陆阳,陈红专.人参炔醇对大鼠脑片不同类型损伤的影响.上海第二医科大学学报,2003,23(6):485
[22]王泽剑,陈红专,薛庆生,等.人参炔醇对大鼠原代培养的神经细胞过氧化氢损伤的影响.中草药2005,36(1):72
[23]Rollinger J M.Zidom C.Dobner M J,et al.Lignans,phenylpropanoids and polyacetylenes from ChaerophyllumaureumL(Apiaceae).ZNaturforsch,2003,58(7-8)..553
[24]Mitsuo Miyazawa,Hideo Shimamura,Rakesh C,et a/.Antimutagenic Activity of Falcarindiol from Peucedanum praeruptorum.Planta Med1982,44(3):129
[1].韩金玉,那平,元英进,亲和色谱纯化蛋白质新进展,色谱1996,14(6):447-450.
[2].黄晰,邱宗荫,免疫亲和色谱定向偶联方法的研究.重庆医科大学学报1999,24(2):132-134.
[3].Ikegawa S,Matsuura K,Sato T,et al.Enantios elective immuno-atTmity extraction for simultaneous determination of optically active bufur alolandits metabolites in human plasma by HPLC[J].J Pharm BiomedAnal,1998,17(1):1-9.
[4].Safaei R.Atarget of the HoxB5 gene from the mouse nervous system[J].Brain Res Dev Brain Res,1997,100(I ):5-12.
[5].Greaser CS,Feely S J,Houghton E,et al.Immunoaffinity chromatography combinedon-line with high-performance liquid chromatography-mass spectrometry for the determination of corticosteroids[J].J Chromatogr A,1998,794(1/2):37-43.
[6].Kussak A,Andersson B,Andersson K,et al.Detennination of a flatoxicol in human urine by immunoaffinity column clean-up and liquid chromatography[J].Chernosphere,1998,36(8):1841-1848.
[7].Kershnar E,Wu SY,Chiang CM.Immunoaffinity purification and ftmctional characterization of human transcription factor Ⅱ Hand RNA polymerise Ⅱ from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes[J].J Biol Chem,1998,237(51):34444-34453.
[8].Zhou D,Zou H,Ni J,et al.Membrane supports as the stationary phase in high-performance immunoaffinity chromatography[J].Anal Chem,1999,71(1):115-118.
[9].Phillips TM,Krum JM.Recycling immunoaffinity chromatography for multipleanalyte analysis in biological samples[J].J Chromatogr B Biomed Sci Appl,1998,715(1):55-63.
[10].Deinl I,Angermaier L,Franzelius C,et al.Simple high-performance liquid chromatographic column-switching technique for the on-line immunoaffinity extraction and analysis of flunitrazepam and its main metabolitesin urine[J].J Chromatogr B Biomed Sci,Appl,1997,704(1/2):251-258.
[11].Suarez AM.Azcona JL.Rodriguez JM,et al.One-step purification of raisin A by immunoailinity chromatography[J].Appl Environ Microbiol,1997,63(12):4990-4992.
[12].Shelves WL,Larsen GL,Huwe JK.Use of anim-muno affinity column for tetrachlorodibenzo-p-dioxin serum sample cleanup[J].J Chromatogr B Biomed Sci Appl,1998,705(2):261-268.
[13].Liang X,Lubman DM,Rossi DT,et al.On-probe immuno affinity extraction by matrix-assisted laser desorption/ionization mass spectrometry[J].Anal Chem,1998,70(3):498-503.
[14].Strong RA,Cho BY,Fisher DH,et al.Immuno-detection approaches and high-performance immuno-aff'mity chromatography for ananalogue of bovine growth hormone releasing Factor attrace levels[J].Biomed Chromatogr,1996,10(6):337-345.
[15].Ruhn PF,Taylor JD,Hage DS.Determination of urinary albumin using high-performance immuno-affinity chromatography and flow injection analysis[J].Anal Chem,1994,66(23):4265-4271.
[16].Cole L J,Kennedy RT.Selective preconcentration for capillaryzone electrophoresis using protein G immunoaffinity capillary chromatography[J].Electrophoresis,1995,16(4):549-556
[17].Simon P,Delsaut P,Lafontaine M,et al.Automated column-switching high-performance liquidchromatography for the determination of aflatoxin[J].J Chromatogr B Biomed Sci,Appl,1998,712(1/2):95-104
[18].Deinl I,Angermaier L,Franzelius C,et al.Simple high-performance liquid chromatographic column-switching technique for theon-line immunoaffinity extraction and analysis of flunitrazepam and its main metabolites in urine[J].J Chromatogr B BiomedSci,Appl,1997,704(1/2):251-258.
[19].Kagel JR,Rossi DT,Nordblom GD,et al.Consideration in the development of a sensitive HPLC assay for human epidermal growth factors in human plasma[J].J pharm BiomedAnal,1995,13(10):1205-1213.
[20].Cai J,Hereon J.Quantitative mufti-residue determination of beta-agonists in bovine urine using on-line immunoaffinity extraction-coupled column packed capillary liquid chromatography-tandem mass spectrometry[J].J Chromatogr B Biomed Sci,Appl,1997,691(2):357-370
[21].Turesky RJ,Garner RC,Welti DH,et al.Meta-holism of the food-borne mutagen2-amino-3,8-dimethylimidazo[4,5-]quinoxaline in humans[J].Chem Res Toxicol,1998,11(3):217-225
[22]邹汉法,黄晓冬,生物分子的分离与检测,色谱,2003,21(4):311-315.
[23].Irving W.Wainer,Roman kaliszan and terence A.GNoctor.Biochromatograpl Using Immobilized Biopolymers:a new approach to he determination of pharmacological properties.J.Pharmacol.1993.45:307-373.
[24].Aubry.A.F.,McGann.A.Application of biochromatography to the determination of drug-protein binding interactions.LC-GC Int.1994,7:389-396.
[25].Domencici.E.,Bertucci,C.,Salvadori,P.,Wainer,1.W.(1991)The use of a human based HPLC chiral stationary phase for the investigation of protein bindin:the allosteric interaction between warfarin and benzo-diazepine binding sites.J.Pharm Sci.1991,80:164-166.
[26].Alleninark,S.Optical resolution by liquid chromatography on immobilized albumin.J.Liq.Chromato gr 1986,9:425-442
[27].Domenici,E.,Bertucci.,C.,Salvadori,P.,Felix,G.,Cahagnd,l.,Motellier,S.,Wainer,1.W.Synthesis and chromatographic oroperties of an HPLC chiral stationary phase based upon human serum albumin.Clmomatographia,1990,29:170-176.
[28].Hermansson,J.Direct liquid chromatographic resolution of racemic drug using α 1-acid glycoprotein as the stationary phase.J.Chromatogr.1983,269:71-80.
[29].Miwa,T.,Ichikawa,M.,Tsuno,M.,Hattori,T,Miyakawa,T,Kayanti,M.,Miyake,Y.Direct liquid chromatographic resolution of racemic compounds use of ovomucoid as a column ligand.Chem.Pharm.Bull.1987,35:682-686.
[30].Haginalca J.,Matsunaga H.Separation of enantiomers on HPLC chiral stationary phases
based on human plasma alphal-acid glycoprotein:effcct of sugar moiety on chiral recognition
ability.Enantiomer,2000,5(1):37
[31].Wainer,IW.,Jadaud,P.,Schonbaum,GR.,Kakookar.S.V.,Henry,M.P.Enzymes as HPLC supports for chiral resolutions:initial investigations with α-chymotrypsin.Chromatographic.1988,25:903-907.
[32].Thelohan,S.,Jadaud,P.,Wainer,I.W.Immobilized enzymes as chromatographic phase for HPLC:the chromatography of tree and derivatized amino acids on trypsin.Chromatographia,1989,28:551-555
[33].Chuff,W.K.,Wainer,l.W.(1992)Enzyme based HPLC supports as probes of enzyme activity and inhihitionahe immobilization of trypsin and α-chymotrypsin on an immobilized artificial membrane HPLC support.Anal.Biochem.1992,201:237-245.
[34].Labrou NE.Improved purification dehydrogenase.Bioseparation.2000;9(2):99-104.
[35].Walsh MK,Nam SH.Affinity enrichment of bovine lactoferrin in whey.Prep Biochem Biotechnol.2001,Aug;31(3):229-40.
[36].Noctor,T.A.G,Felix,G.,Wainer,I.W.Stereochemical resolution of enantiomeric 2-aryl propionic acid non-steroidal anti-inflammatory drug on a human serum album based high performance liquid chromatographic chiral stationary phase.Chromatographia.1991,31:55-59.
[37].Allenmark S.Chromatographic Enantioseparation:Methods and Applications.2nd Edition.New York:Ellis Horwood,1991,chapter 7.
[38].YANG Yongjian,Hou Meiqin.Enantiomeric separation of salmeterol by HPLC using αl-acid glycoprotein chiral stationary phase.Chin JPharm Anal,2001,21(2):97.
[39].Gyimesi-Forms K,Szasz G,Gergely A,et al.Optical resolution of a series of potential cholecystokinin antagonist 4(3H)-quinazolone dekivatives by chiral liquid chromatography on alphal acid glycoprotein stationary phase.J Chromatogr.Sci,2000,38(10):430.
[40].Jadaud P,Thelohan S,Schonbaum CR,et al.The stereochemical resolution of enantiomeric free and derivatized amino acids using an HPLC chiral stationary phase based structure on immobilized or enzymealpha-chymotrypsin:chiral separation due to solute activity.Chirality,1989,1(1):38.
[41].Henriksson H,Munoz IG,Isaksson R,et al.Cellobiohydrolase 58(P.c.Ce17D) is complementary to the homologouse CBHI(T.r.Cel7A) in enantioseparations.J Chromatogr.A.2000,898(1):63.
[42].Divne C,Stahiberg J,Teeri T,et al.High-resolution crystal structures reveal how a cellulose chain is bound in the 50A long tunnel of cellobiohydrolase I from Trichoderma reesei.J Mol Biol.1998,275(2):309.
[43].Mao N,Oda Y,Takakuwa S,et al.Plasma direct injection high-performance liquid chromatograpgic method for simultaneously determining E3810 enantiomers and their metabolites by using flavoprotein-conjugated column.J Pharm Sci,1996,85(9):903.
[44].Vermeulen B,Remon JP.Validation of a high-performance liquid chromatographic method for the determination of ibuprofen enantiomers in plasma of broiler chickens.J Chromatogr(Netherlands),2000,749(2):243.
[45].Ceccato A,Hubert P,de Tullio P,et al.Simultaneous determination of pirlindole enantiomers and dehydropirlindol by chiral liquid chromatography.J Pharm Biomed Anal,1998,17(67):1071.
[46].Domencici.E.,Bertucci,C,Salvadori,P,Felix,G,Cahagnd,I,Motellier,S.,Wainer,LW.Lmmobilized serum alburnin:a rapid probe of stereoselective protein binding interactions.Chirality.1990,2:263-268.
[47].Domencici.E.,Bertucci,C,SaIvadori,R,Wainer,LW.The use of a human serum albumin based HPLC chiral stationary phase for the investigation of protein binding:the detection of the allosteric interaction between warfarin and benzo-diazepine binding sites.J.Pharm Sci.1991,80:164-166.
[48].Noctor,T.A.G,Hage.D.S.,Wainer,1.W.(1992).Human serum albumin based chiral stationary phase for high performance liquid chromatography:a tool for the rapid study of drug-protein binding interactions.J.Chromatogr,577:305-315.
[49].Noctor,T.A.G,Diaz-Perez,MJ.,Wainer,I:W.(1993).The use of a human serum albumin based stationary phase for-high performance liquid chromatography as a tool for the rapid determination of drug-plasma protein binding.J.Pharm.Sci.1993.
[50].Kaliszan,R.,Noctor.T.A.G,Wainer,IW.Stereochemical aspects of benzodiazepine binding to human serum album.II.Quantitative relationships between structure and enantioselective retention in high performance liquid chromatography.Mol.Pharmacol. 1992,42:512-517.
[51].Noctor,T.A.G.,Cuong,D.P.,Kaliszan,R.,Wainer,I.W.Stereochemical aspects of benzodiazepine binding to human serum albtrm.I.Enantioselective high performance liquid chromatography examination of chiral binding interactions between 1,4-BDZ and human serum albumin.Mol.Pharmacol.1992,42:506-511.
[52].Das,A.S.,Singh,S.,Raghubanshi,R.S.,Jayalswal.S.B.,Singh,J.Effect of drugs in vitro captopril protein binding.Phannazie.1989,44:503-504.
[53].Iieire,D.A.,Santhana Mariappan,S.V.,Peng,J.,Rabenstein,D.L.Nuclear magnetic studies of the binding of captopril and penicillamin by serum albumin.Biochem.Pharmacol.1993,46:1059-1069.
[54].Anne,Francoise Aubry,et al.The effect of Co-administered drugs on Oxaprozin binding to human serum albumin.1995,47:937-944.
[55].Kaliszan R,Nasal A,Turowslcim.Binding site for basic drugs on aiphal-acid glycoprotein a by chemometric anylysis of biochromatographic data.Biomed Chromatogr:1995,9(5):211-215.
[56]孔亮,邹汉法,汪海林等.以人血清白蛋白为固定相的分子生物色谱分析几种中药活性成分的研究[J].高等学校化学学报,2000,21(1):3640.
[57].Wang HL,Kong L,Zou HF,et al.Screening and analysis of biologically active compounds in Angelica sinensis by molecular biochromatography[J].Chromatographic,1999,50(7/8):394-445.
[58].Wang H,Zou H,Ni J,et al.Comparative separation of biologically active components in Rhizoma chuanxiong by aff'mity chromatography with α_1-acid glycoprotein and human serum albumin as stationary phases[J].Chromatographic,2000,52(718):459-464.
[59].Wang H,Zou H,Ni J,et al.Fractionation and analysis ofArtemisia capillaris Thunb.by affinity chromatography with human serum albumin as stationary phase[J].J Chromatogr A,2000,870:501-510.
[60].Wang H,Zou H,Kong L,et al.Analysis of bioactive components in traditional Chinese medicines by molecular biochromatography with α＿1-acid glycoprotein as stationary phase [J].Basic Clin Physiol Pharmacol;2000,11(2):155-172.
[61].汪海林,邹汉法,孔亮等.分子生物色谱用于中药活性成分筛选及质量控制方法的研 究[J].色谱1999,17(2):123-127
[62].Hailin Wang,Liang kong,Hanfa Zou,et al.Screening and analysis of biologically active compounds in Angelica sinensis by Molecular biochromatography.Chromatographic.Vol 50.No.718.October.1999.439-445.
[63].Wang H,Zou H,Kong L,Ni J.Analysis of bioactive components on traditional Chinese medicines by molecular biochromatography with alphal-acid glycoprotein stationary phase.Basic Clin physiol Pharmacol.2000:11(2):155-172.
[64]郭明,孔亮,厉欣,邹汉法.微透析探针的制备及其在Ni(Ⅱ)与人血清白蛋白的相互作用研究中的应用色谱,2003,21(2):147.
[65]毛希琴,邹汉法,封顺,孔亮,黄晓冬,厉欣,罗权舟.3种色谱模式联用在中药活性成分初步筛选中的应用.2003分析化学,31(8):992-995.
[66]汪海林,邹汉法,孙生才,张玉奎微透析取样技术及其在生化分析中的应用。色谱,1996.14(6):441.
[67].Cai S J,McAndrew R S,Leonard B P,et al.Rapid purification of cotton seed membrane-bound N-acylphosphatidylethanolamine synthase by immobilized artificial membrane chromatography[J].J Chromatogr A,1995,696:49-62.
[68].Yang Q,Wallsten M,Lundahl P.Lipid-vesicle-surface chromatography[J].1 Chromatogr,1990.506:379-389.
[69].Yang Q,Lundahl P.Binding of lysozyme on the surface of entrapped phosphatidylserine-phosphatidylcholine vesicles and an example of high-performance lipid vesicle surface chromatography[J].J Chromatogr,1990,512:377-386.
[70].Pidgeon C,Cai S J,Bemal C.Mobile phase effects on membrane protein elution during immobilized artificial membrane chromatography fJ].J Chromatogr A,1996,721:213-230.
[71]Hanna M,Biasi V D,Bond B,et al.Biomembrane lipids as components of chromatographic phases:comparative chromatography on coated and bonded phases[J].Chromatographic,2000.52(11/12):710-720.
[72].Lundahl P,Yang Q.Liposome chromatography:liposomes immobilized in gel beads as a stationary phase for aqueous column chromatography[J].J Chromatogr,1991,544:283-304.
[73].Klein E.Affinity membranes:a 10-year review[J].J Membr Sci,2000,179(1-2):1-27.
[74].毛希琴,邹汉法,罗权舟等.模拟生物膜色谱用于预测药物的小肠吸收[J]_分析化学, 2001,29(10):1135-1139.
[75].Pidgeon C,Venkataram U.V.Immobilized artificial membrane chromatography:supports composed of membrane lipids[J].Anal Biochern,1989,176:36-47.
[76].毛希琴,邹汉法,罗权舟,孔亮,厉欣,孙乃昌.卵磷脂涂敷生物膜色谱固定相的制备及其稳定性的考察。色谱,2001,19(5):433.
[77].贺浪冲,杨广德,耿信笃,固定在硅胶表而细胞膜的酶活性及其色谱特性。科学通报,1999,44(6):632-637.
[78].He LC,Wang SC,Geng XD.Coating and fusing cell membrane onto a silica surface and their chromatographic characteristic[J].Chromatographia,2001,54:77.
[79]He LC,Yang GD,Geng XD.Enzymatic and chromatophic characteristics of the cell membrane immobilized on silica surface.Chinece Science Bulletic,1999,44(9):826.
[80].Brekkan E,Lundqvist A,Lundahl P.Immobilized membrane vesicle or proteoliposome affinity chromatography.Frontal analysis of interaction of cytochatasin L and D-glucose with the human red cell glucose transporter[J].Biochern,1996,35:12141-12145.
[81].Beigi E Lundahl P.Immobilized biomembrance chromatography of highly lipophilic drugs[J].J ChromatogrA,1999,852:313-317.
[82].董自波,红细胞膜生物色谱法的建立及其在当归效应物质研究中的应用。南京中医药大学博士研究生论文,2002.5.
[83].张汉利,杨广德,贺浪冲,杨银京。太白花活性成分的筛选与药理作用相关性研究。中国药学杂志2003,38(2):92-94
[84].赵小娟,党高潮,杨广德,贺浪冲.淫羊蕾根活性成分的分析和比较[J].分析化学2002,30(2):195-197.
[85].高现,贺浪冲,杨广德.用细胞膜色谱法筛选研究红毛七中的有效成分.中国药学杂志2003,38(1):14-16.
[86].赵惠茹,杨广德,贺浪冲.用细胞膜色谱法筛选当归中的有效成分[J].中国药学杂志2000,35(1):13-15.
[87].贺浪冲,耿信笃.细胞膜受体色谱法研究药物与受体作用的新方法[J].生物医药色谱新进展,1996,3:8.
[88].HOU Jin,YIJAN,BINGXiang,HE Lang Chong,et al,Evaluation of drug muscarinic receptor affinities by using cell membrane chromatography.Chin J Pharmacol Toxico l.2003,17(1):70-73.
[89].HE Lang-chong,WANG Si-cen,GENG Xin-du.Coating and fusing cell membrane onto a silica surfuse and their chromatographic characteristics.Chromatographia,2001,54-71,
[90].Zeng C M,Zhang Y X,Lu L L,et al.Immobilization of human red cells in gel particles for chromatographic activity studies of the glucose transporter Glut I[J].Biochem Biophys Acta,1997,1325(1):91-98.
[91].Muszynska G,Andersson L,Porath J.Selective adsoption of phosphoproteins on gel-immobilized ferricchelate.Biochemistry,1986,25(22):6850-6853.
[92].Zhou W,Merrick BA,Khaledi MG,Tomer KB.Detection and sequencing of phosphopeptide aff'mity bound to immobilized metal ion beads by matrix assisted desorption/ionization mass spectrometry.Jam Soc Mass Spectrom,2000,11(4):273-282.
[93].Stensballe A,Andersen S,Jensen ON.Characterization of phosphoproteins from electrophoretic gels by nanoscale Fe(Ⅲ) affinity chromatography with off-line mass spectrometry analysis.Proteornics,2001,1(2):207-222.
[94].大连理工大学无机化学教研室,无机化学,第三版—北京,高等教育出版社,1990,349:384.
[95].Ueda EK,Gout PW,Morganti L.Ni(Ⅱ)-based immobilized metal ion affinity chromatography of recombinant human prolactin from periplasmic Escherichia coli extracts.J ChromatogrA.2001 Jul 13;922(]-2):165-75.
[96].程慧文,邵天梦,鳌合金属离子亲和色谱法分离α-氨基酸和肽[J],色谱1997,15(5):405-407.
[97].邸泽梅,陈国亮,雷建都,李蓉,李华儒.金属鳌合亲和色谱介质的合成及其色谱特性研究,色谱1998,16(4):297-300.
[98].李蓉,邸泽梅,陈国亮,金属鳌合亲和色谱中固定金属与蛋白质的作用,分析化学2002,30(5):552-555.
[99],Slentz BE,Penner NA,Regnier.Protein protedysis and the multi dimensional electrochromatographic separation of histidine containing peptide fragments on a chip.J Chromatogr A.2003,984:97.
[100].Riggs L,Sioma C,Regnier FE.J ChromatogrA,2001,924:359.
[101].Luo QZ,Zou HF,Zhang Q,Xiao XZ et aI.J ChromatogrA,2001,926:255
[102].Larsen MR,Soren GL.,Fey SJ,Larsen PM,Roepstorff E Phosphoproteomics:Evalution of the use of enzymatic dephosphorylation and differential mass spectrometric peptide mass yapping for site specific phosphorylation assigrrmentin proteins separated by gel electrolhresis.Proteomics,2001,1(2):223-238.
[103].McLachlin DT,Chait BT.Analysis of phosphorylated proteins and peptides by mass spectrometry.Curr Opin Chem Biol,2001,5(5):591-602.
[104].王京兰,张养军,蔡耘,李蕾,刘尚义,杨何义,钱小红.生物质谱结合 IMAC 亲和提取和磷酸酶水解分析蛋白质磷酸化修饰。生物化学与生物物理学报2003,35(5):459-466.
[105].高勤,左文坚。免疫亲和色谱及其在生物样本分析中的应用,国外医学药学分册2000,27(2):107-111
[106].叶明亮,邹汉法,刘震,雷政登,倪坚毅.开管毛细管亲和液相色谱初探.色谱,2000,18(6):529-531
[107].何新亚,林炳承,丁永生。亲和毛细管电泳研究生物分子的手性识别。化学进展,2002;14(5):332.
[108].潘再法,莫卫民,吴仁安.蛋白 A 连续床亲和毛细管色谱柱的制备及性能考察,高等学校化学学报,2002,23(11):2070-2072.
[109].Rocklin RD,Rainsey RS,Ramsey JM.A microfabricated fluidic device for performing two-dimensional liquidic-phase separations.Anal Chem,2000,72:5244-5247.
[110].Zhang X,Hu X,XuS,et al.Comprehensive two-dimensionalcapillaryLC and CEfor resolution of neutral components in traditional Chinese medicines.J Sep Sci 2001,24:385-391.
[1]苏海涯,吴越明,刘建新.桑叶中的营养物质和生物活性物质[J].饲料研究,2001
[2]朱祥瑞,陆洪省.桑叶化学成分及其药用价值[J].全国桑柞茧丝新资源开发利用研讨会论文集,2006
[3]金丰秋。新型功能性饮品—桑茶[J].食品科学,2000,21(1):46
[4]Kim Sy.Antionxiative Flavonoids from the leaves ofMoms alba L.[J].Arch Pharm Res,1999,22(1):81
[5]Kayo Doi.Studies on the constituent of the leaves of Moms alba L.[J].Chem Pharm Bull,2001,49(2):151-153
[6]陈福君,卢军,张永煜.桑的药理研究(I):桑叶降糖有效组分对糖尿病动物代谢的影响[J].沈阳药科大学学报,1996,13(1):24
[7]张琳华,高瑞旭,许明丽.桑叶中多糖提取分离工艺的研究[J].中草药,2005,36(4)
[8]Yoshiaki Yoshikuni.Inhibition of α-glucosidase activity and postprandial hyperglycemia by moraline and its N-alkyl derivatives[J].Agric Biol Chem.,1988,52(1):121-128
[9]Asano N.Sugars with Nitrogen in the Ring Isolated in the Leaves ofMoms Bombycis[J].Carbohydr Res,1994,253:235
[10]邹盛勤,陈武.桑叶的化学成分、药理活性及应用研究进展[J].林业加工通讯,2003,37(1):22-25
[11]王芳,励建荣。桑叶的化学成分、生理功能及应用研究进展[J].食品科学,2005,26(增刊):111
[12]Arai M,Minatoguehi S,Takemure G,et.N-methyl-l-deoxynojirimycin(MOR-14),an α-glucosidase inhibitor,markedly reducedinfarct size in rabbit hearts[J],Circulation.1998,97(13):1290-1293[13]Kimura M,Chen F.Antihyperglycemic effects of N-containing sugar derived from mulberry leaves in strep tozocin-induced diabetic mice[J],Wakan Iyakugaku Zasshi,1995,12(3):214-219
[14]TaniguchiS.AsanoN.TominoF.et al.Polyhydroxylated of glucose-induced insulin secretion by fagomine,a pseudo-sugar isolatedfrom mulberry leaves[J].Horm Metab Res.1998,30(1):676-683
[15]贾之慎.唐孟成,朱祥瑞.桑叶黄酮类化合物清除超氧离子自由基的研究[J].浙江农业大学学报.1996.22(5):519-523
[16]Doi K,Kojima T,Fujimoto Y.Mulberry leaf extract inhibits the oxidative modidication of rabbit and human low density lipoprotein[[J].BiolPharm Bull.2000,23(9):1066-1071
[17]武深秋.养生保健话桑叶[J].药膳食疗,2003,(1):76
[18]居跃君,袁茂云,吴秀玲.平肝安神汤治疗高血压病伴失眠症102例[J].辽宁中医杂志,2003,30(7):548-549
[19]孙秀凤.桑菊饮加味治疗喉源性咳嗽60例[J].陕西中医,2003,24(4):305-306
[20]颜治,屠大伟,郑诗超等.桑叶碳酸饮料的制作工艺研究[J].广州食品工业科技.2003,19(2):49-50
[21]李莉.桑叶保健酒的研制[J].湖北民族学院学报(自然科学版).1997,15(6):34-35
[22]One K.Inhibition of reverse transcriPtase activity by a flavonoid compound 5,6,7-trihydroxynavone.Biochem.Bio-Phys.Res.Commun.1989,160(3):982-984
[23]Groweiss A.桑科植物Maclura tinctoria中具有抗HIV活性的异戊烯基山酮和黄酮类化合物。国外医药-植物药分册,2001,16(6):261
[24]Kim S.Y.Gao J.J,Kang H.K.Two flavonoids from the leaves ofMorus alba induce differentiation of the human promyelocytic leukemia(HL-60)cell line[J].Biolpharm Bull,2000,23(4):451-455
[25]华晓珊,两地汤加减治疗室女崩漏47例[J],陕西中医,2001,22(6):329
[26]陈军,王明智,治疗放射性肺炎的润肺通络法[J].江南大学学报(自然科学版).2002,1(4):435-436
[27]江苏新医学院,中药大辞典(下册)[M],上海:上海人民出版社,1997、
[28]金丰秋,金其荣,新型功能性饮品—桑茶[J],食品科学,2000,21(1):46
[1]HANSJORG D1ETZ and PETER WINTERHALTER.Phytotoxic constituens form Bunias orientalis leaves.Phytochemistry,Vol.42,No.4,pp.1005-1010,1996
[2]Maclas,F.A.,Oliva.et aL Allelochemicals from sunflower leaves cv.Peredovick.Phytochemistry 1999(52),613-621.
[3]KIMURA J,MAKI N.New loliolide derivatives from the Brown Alga Undaria pinnatifida[J].JNat Prod,2002,65(1):57-58
[4]Oliver K,Herbert K.Highly oxygenated coumarins from Pelargonium sidoides[J].Phytochemistry,1995,39(5):1181
[5]Kitajima J,Ishikawa T,Tanaka Y,et al.Water-soluble constituents of Fennel.V.Glycosides of aromatic compounds[J].ChemPharmBull,1998,46(10):1587.
[6]Hongquan Duan,Yoshihisa Takaishi,,Hiroshi Momota.et al.Immunosuppressive constituents from Saussurea medusa.Phytochemistry 59(2002) 85-90
[7]Tan J J,Jiang S H,Zhu D Y.Studies on the chemical constituents ofPleurospermum lindleyanum [J].Nat ProdResDev,2005,17(3):267-271.
[8]程志红,吴瞍,余伯阳.麦冬块根化学成分的研究,天然产物研究与开发,2005,17(1),1-3
[9]程志红,吴搜,余伯阳.冬块根化学成分的的研究[J].无然产物研究与开发,2005,17(1):1.
[10]王双明,谭宁华,杨亚滨,等.七环二肽成分[J].无然产物研究与开发,2004,16(5):383.[11]Agrawal PK,Rastogi RPR.~(13)CNMR spectral analysis of dihydmbenzofuran lignans[J].Org Res,1983,21(2):119-121.
[12]Thomas P,OlofT.The constituents ofc0nifer needles.VI:Phenolic glycosides from Pinus sylvestris [J].ActaChemScandB,1977,31:329-337.
[13]吴立军,郑健,姜宝虹等.刺五加茎叶化学成分.药学学报,1999,34(4):294-296
[14]刘桂芳,王连.瑞香狼毒化学成分研究[J].中国中药杂志20(12),1995
[15]向兰,郭东晓,鞠瑞等.马齿苋中环二肽成分研究[J].中草药,2007,38(11)1622-1625
[16]SU Dong-min,TANG Wen-zhao,YU Shi-shan,et al.Water-soluble constituents from roots of Capparis tenera.China journal of Chinese material medica.33(9),1021-1023
[17]Alan J J,David MG,Michae WW,et al.Carbon-13 magnetic resonacne XVII pyrimidine and purine nucleosides.JAmer Chem Soc,1970,92:4079.
[18]Sadtler Research,Laboratories Standard infrared grating spectra[M].Philadelphia USA.Sadtler Research Laboratorieies Lnc.1988,67,491-493