犬慢性心肌梗塞制备与功能、代谢、形态及灌注的评价
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摘要
缺血性心脏病在当今世界上，尤其在工业发达的国家，是最常见的病死原因之一。长期以来，人们借助心肌缺血和／或梗塞动物模型，对其发生、发展以及疗效评价做了大量研究，取得了很大成果。但是，既往的动物模型，多为开胸阻断冠状动脉的主要分支或采用闭胸技术的短期观察，因而实验结果与临床状况相距甚远。心肌缺血和再灌注损伤是心脏病学应用基础研究中的一个热点。但是，大多数实验仍停留在对急性心肌缺血、梗塞和再灌注的研究。为此，有必要建立动物慢性心肌梗塞模型，对缺血和／或梗塞心肌的功能、代谢、形态及灌注做进一步的研究。
本研究的目的
(1) 建立犬闭胸慢性心肌梗塞模型，研究实验方法。
(2) 测量急性及慢性冠脉狭窄心肌损害时血清CK-MB及铜锌含量改变。
(3) 通过检测血液动力学的变化，观察不同程度冠脉狭窄对左室收缩与舒张功能的影响。
(4) 不同冠脉狭窄时左室整体及局部室壁运动的评价。
(5) 慢性心肌缺血范围及心肌灌注的评价。
(6) 电影磁共振成像方法的比较，探讨临床应用可行性。
(7) ~(31)P MRS检测技术的应用，评价其优缺点。
(8) 观察急、慢性心肌缺血和／或梗塞心肌病理改变。
研究方法：
(1) 采用Seldinger技术，分别建立犬LCX或LAD主干内狭窄，制
Ischemic heart disease (IHD) is the most common cause of morbidity and mortality in the modern world, especially in the industrial countries, For quite a long time, a great number of scientific works have been engaged in the research about many aspects including pathology, physiology and treatment of the disease by means of animal models of myocardial ischemia and/or infarction with great success. The point is that previous animal models of IHD are, to a great extent, acute occlusions of branches of left coronary artery performed by open- chest operation. Some models are closed-chest but short in observation time, ischemia-reperfusion injury is a hot point in applied basic research in cardiology. The researches for IHD slow down at stage for a acute ischemia, infarction and reperfusion for the reasons mentioned-above. In order to
本研究的目的
(1) 建立犬闭胸慢性心肌梗塞模型，研究实验方法。
(2) 测量急性及慢性冠脉狭窄心肌损害时血清CK-MB及铜锌含量改变。
(3) 通过检测血液动力学的变化，观察不同程度冠脉狭窄对左室收缩与舒张功能的影响。
(4) 不同冠脉狭窄时左室整体及局部室壁运动的评价。
(5) 慢性心肌缺血范围及心肌灌注的评价。
(6) 电影磁共振成像方法的比较，探讨临床应用可行性。
(7) ~(31)P MRS检测技术的应用，评价其优缺点。
(8) 观察急、慢性心肌缺血和／或梗塞心肌病理改变。
研究方法：
(1) 采用Seldinger技术，分别建立犬LCX或LAD主干内狭窄，制
Ischemic heart disease (IHD) is the most common cause of morbidity and mortality in the modern world, especially in the industrial countries, For quite a long time, a great number of scientific works have been engaged in the research about many aspects including pathology, physiology and treatment of the disease by means of animal models of myocardial ischemia and/or infarction with great success. The point is that previous animal models of IHD are, to a great extent, acute occlusions of branches of left coronary artery performed by open- chest operation. Some models are closed-chest but short in observation time, ischemia-reperfusion injury is a hot point in applied basic research in cardiology. The researches for IHD slow down at stage for a acute ischemia, infarction and reperfusion for the reasons mentioned-above. In order to
引文
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